https://www.bosterbio.com/products/primary-antibodies/anti-mre11-picoband-trade-antibody-a00731-2-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00731-2-MRE11-primary-antibodies-WB-testing-1.jpgAnti-MRE11/MRE11 Antibody Picoband® Western blot analysis of MRE11 using anti-MRE11 antibody (A00731-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human MCF-7 whole cell lysates,<br>Lane 3: human COLO-320 whole cell lysates,<br>Lane 4: human U-87MG whole cell lysates,<br>Lane 5: rat brain tissue lysates,<br>Lane 6: rat liver tissue lysates,<br>Lane 7: mouse brain tissue lysates,<br>Lane 8: mouse liver tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRE11 antigen affinity purified polyclonal antibody (Catalog # A00731-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRE11 at approximately 81KD. The expected band size for MRE11 is at 81KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00731-2-MRE11-primary-antibodies-IHC-testing-2.jpgAnti-MRE11/MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A00731-2).
<br>MRE11 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MRE11 Antibody (A00731-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00731-2-MRE11-primary-antibodies-IHC-testing-3.jpgAnti-MRE11/MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A00731-2).
<br>MRE11 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MRE11 Antibody (A00731-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00731-2-MRE11-primary-antibodies-IHC-testing-4.jpgAnti-MRE11/MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A00731-2).
<br>MRE11 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MRE11 Antibody (A00731-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-mad-picoband-trade-antibody-a06485-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06485-1-Mad-primary-antibodies-WB-testing-1.jpgAnti-Mad/MXD1 Antibody Picoband® Western blot analysis of Mad using anti-Mad antibody (A06485-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse ovary tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mad antigen affinity purified polyclonal antibody (Catalog # A06485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mad at approximately 28KD. The expected band size for Mad is at 25KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mybpc3-picoband-trade-antibody-a01078-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01078-1-mybpc3-primary-antibodies-wb-testing-1.jpgAnti-MYBPC3 Antibody Picoband® Western blot analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates,<br>Lane 2: mouse heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBPC3 antigen affinity purified polyclonal antibody (Catalog # A01078-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYBPC3 at approximately 160KD. The expected band size for MYBPC3 is at 147KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01078-1-MYBPC3-primary-antibodies-IHC-testing-4.jpgAnti-MYBPC3 Antibody Picoband® IHC analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1).
<br>MYBPC3 was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYBPC3 Antibody (A01078-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01078-1-MYBPC3-primary-antibodies-IHC-testing-3.jpgAnti-MYBPC3 Antibody Picoband® IHC analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1).
<br>MYBPC3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYBPC3 Antibody (A01078-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01078-1-MYBPC3-primary-antibodies-IHC-testing-2.jpgAnti-MYBPC3 Antibody Picoband® IHC analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1).
<br>MYBPC3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYBPC3 Antibody (A01078-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01078-1-MYBPC3-primary-antibodies-IHC-testing-5.jpgAnti-MYBPC3 Antibody Picoband® IHC analysis of MYBPC3 using anti-MYBPC3 antibody (A01078-1).
<br>MYBPC3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYBPC3 Antibody (A01078-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-nedd4-2-picoband-trade-antibody-a01595-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01595-1-NEDD4-2-primary-antibodies-WB-testing-1.jpgAnti-NEDD4-2/NEDD4L Antibody Picoband® Western blot analysis of NEDD4-2 using anti-NEDD4-2 antibody (A01595-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human 22RV1 whole cell lysates,<br>Lane 2: human Jurkat whole cell lysates,<br>Lane 3: rat pancreas tissue lysates,<br>Lane 4: mouse pancreas tissue lysates,<br>Lane 5: mouse NIH3T3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4-2 antigen affinity purified polyclonal antibody (Catalog # A01595-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4-2 at approximately 130KD. The expected band size for NEDD4-2 is at 112KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01595-1-nedd4-2-primary-antibodies-fc-testing-2.pngAnti-NEDD4-2/NEDD4L Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-NEDD4-2 antibody (A01595-1).<br> Overlay histogram showing U20S cells stained with A01595-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4-2 Antibody (A01595-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-nadph-oxidase-4-picoband-trade-antibody-a00403-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00403-nox4-primary-antibodies-wb-testing-2_1.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband®Western blot analysis of NOX4 using anti-NOX4 antibody (A00403).<br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br>
Lane 1: human BEAS-2B whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen polyclonal antibody (A00403) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 67 kDa. The expected band size for NOX4 is at 67 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00403-41598_2025_85658_fig4_html.pngAnti-NADPH oxidase 4/NOX4 Antibody Picoband®The oxidative stress levels in the kidney tissue of db/db mice. ( A – B ) The T-SOD and CAT activities in kidney tissue ( n = 5). ( C ) The 8-hydroxy-2 deoxyguanosine (8-OHDG) in serum ( n = 6). ( D – E ) The MDA and LPO content in kidney tissue ( n = 5). ( F – G ) The expression and statistics of NOX-4, 4-HNE and 12-Lox proteins in kidney tissues ( n = 6). The results are presented as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. db/m group. # p < 0 05, ## p < 0.01 vs. db/db group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-85658-z'>39799153</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00403-nox4-primary-antibodies-wb-testing-1.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband® Western blot analysis of NOX4 using anti-NOX4 antibody (A00403). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human U251 whole cell lysates,<br>
Lane 5: human U2OS whole cell lysates,<br>
Lane 6: human Hela whole cell lysates,<br>
Lane 7: human T47D whole cell lysates,<br>
Lane 8: monkey COS-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 65 kDa. The expected band size for NOX4 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00403-nox4-primary-antibodies-if-testing-2.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband® IF analysis of NOX4 using anti-NOX4 antibody (A00403). <br>
NOX4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOX4 Antibody (A00403) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-neuropeptide-s-picoband-trade-antibody-a01290-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01290-1-nps-primary-antibodies-ihc-testing-1.jpgAnti-Neuropeptide S/NPS AntibodyIHC analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1). <br>Neuropeptide S was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuropeptide S Antibody (A01290-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01290-1-nps-primary-antibodies-ihc-testing-2.jpgAnti-Neuropeptide S/NPS AntibodyIHC analysis of Neuropeptide S using anti-Neuropeptide S antibody (A01290-1). <br>Neuropeptide S was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuropeptide S Antibody (A01290-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-plau-picoband-trade-antibody-a04352-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04352-1-PLAU-primary-antibodies-WB-testing-1.jpgAnti-PLAU Antibody Picoband® Western blot analysis of PLAU using anti-PLAU antibody (A04352-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat pancreas tissue lysates,<br>Lane 2: mouse pancreas tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAU antigen affinity purified polyclonal antibody (Catalog # A04352-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAU at approximately 48KD. The expected band size for PLAU is at 48KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rab27a-picoband-trade-antibody-a01608-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01608-1-RAB27A-primary-antibodies-WB-testing-1.jpgAnti-RAB27A Antibody Picoband® Western blot analysis of RAB27A using anti-RAB27A antibody (A01608-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human Jurkat whole cell lysates,<br>Lane 3: human MCF-7 whole cell lysates,<br>Lane 4: human HepG2 whole cell lysates,<br>Lane 5: human A549 whole cell lysates,<br>Lane 6: rat stomach tissue lysates,<br>Lane 7: rat thymus tissue lysates,<br>Lane 8: mouse thymus tissue lysates,<br>Lane 9: mouse NIH3T3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27A antigen affinity purified polyclonal antibody (Catalog # A01608-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01608-1-RAB27A-primary-antibodies-IHC-testing-2.jpgAnti-RAB27A Antibody Picoband® IHC analysis of RAB27A using anti-RAB27A antibody (A01608-1).
<br>RAB27A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB27A Antibody (A01608-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01608-1-RAB27A-primary-antibodies-IHC-testing-3.jpgAnti-RAB27A Antibody Picoband® IHC analysis of RAB27A using anti-RAB27A antibody (A01608-1).
<br>RAB27A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB27A Antibody (A01608-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01608-1-rab27a-primary-antibodies-ihc-testing-4.jpgAnti-RAB27A Antibody Picoband® IHC analysis of RAB27A using anti-RAB27A antibody (A01608-1).<br>
RAB27A was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB27A Antibody (A01608-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01608-1-rab27a-primary-antibodies-ihc-testing-5.jpgAnti-RAB27A Antibody Picoband® IHC analysis of RAB27A using anti-RAB27A antibody (A01608-1).<br>
RAB27A was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB27A Antibody (A01608-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01608-1-rab27a-primary-antibodies-ihc-testing-6.jpgAnti-RAB27A Antibody Picoband® IHC analysis of RAB27A using anti-RAB27A antibody (A01608-1).<br>
RAB27A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB27A Antibody (A01608-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01608-1-rab27a-primary-antibodies-fc-testing-7.jpgAnti-RAB27A Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RAB27A antibody (A01608-1).<br>Overlay histogram showing K562 cells stained with A01608-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB27A Antibody (A01608-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rxra-picoband-trade-antibody-a01299-1-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01299-1-RXRA-primary-antibodies-WB-testing-1.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® Western blot analysis of RXRA using anti-RXRA antibody (A01299-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human COLO-320 whole cell lysates,<br>Lane 3: human A431 whole cell lysates,<br>Lane 4: human MCF-7 whole cell lysates,<br>Lane 5: rat heart tissue lysates,<br>Lane 6: mouse heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RXRA antigen affinity purified polyclonal antibody (Catalog # A01299-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RXRA at approximately 51-55KD. The expected band size for RXRA is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-1-rxra-primary-antibodies-ihc-testing-5.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband®IHC analysis of RXRA using anti-RXRA antibody (A01299-1). <br>RXRA was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RXRA Antibody (A01299-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01299-1-RXRA-primary-antibodies-IHC-testing-2.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® IHC analysis of RXRA using anti-RXRA antibody (A01299-1).
<br>RXRA was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RXRA Antibody (A01299-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01299-1-RXRA-primary-antibodies-IHC-testing-3.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® IHC analysis of RXRA using anti-RXRA antibody (A01299-1).
<br>RXRA was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RXRA Antibody (A01299-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-1-rxra-primary-antibodies-ihc-testing-4.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® IHC analysis of RXRA using anti-RXRA antibody (A01299-1). <br>
RXRA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RXRA Antibody (A01299-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-1-rxra-primary-antibodies-if-testing-5.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® IF analysis of RXRA using anti-RXRA antibody (A01299-1). <br>
RXRA was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RXRA Antibody (A01299-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-1-rxra-primary-antibodies-fcm-testing-6.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-RXRA antibody (A01299-1). <br>
Overlay histogram showing A431 cells stained with A01299-1 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RXRA Antibody (A01299-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-s100a10-picoband-trade-antibody-a02787-2-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-wb-testing-1.jpgAnti-S100A10 Antibody Picoband® Western blot analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human Hacat whole cell lysates,<br>
Lane 4: human U87 whole cell lysates,<br>
Lane 5: monkey lung tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A10 antigen affinity purified polyclonal antibody (Catalog # A02787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A10 at approximately 11 kDa. The expected band size for S100A10 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-2.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-3.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-4.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-5.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-6.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-ihc-testing-7.jpgAnti-S100A10 Antibody Picoband® IHC analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-if-testing-8.jpgAnti-S100A10 Antibody Picoband® IF analysis of S100A10 using anti-S100A10 antibody (A02787-2).<br>
S100A10 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-if-testing-9.jpgAnti-S100A10 Antibody Picoband® IF analysis of S100A10 using anti-S100A10 antibody (A02787-2). <br>
S100A10 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-S100A10 Antibody (A02787-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02787-2-s100a10-primary-antibodies-fcm-testing-10.jpgAnti-S100A10 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-S100A10 antibody (A02787-2). <br>
Overlay histogram showing A431 cells stained with A02787-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A10 Antibody (A02787-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-sdhb-picoband-trade-antibody-a01090-boster.html2026-03-24T05:16:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-wb-testing-1_1.jpgAnti-SDHB Antibody Picoband®Western blot analysis of SDHB using anti-SDHB antibody (A01090). <br>
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human THP-1 whole cell lysates,<br>
Lane 5: rat heart tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHB antigen affinity purified polyclonal antibody (A01090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29 kDa. The expected band size for SDHB is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-ihc-testing-1.jpgAnti-SDHB Antibody Picoband®IHC analysis of SDHB using anti-SDHB antibody (A01090). <br>SDHB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (A01090) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-ihc-testing-2_1.jpgAnti-SDHB Antibody Picoband®IHC analysis of SDHB using anti-SDHB antibody (A01090). <br>SDHB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (A01090) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-ihc-testing-3_1.jpgAnti-SDHB Antibody Picoband®IHC analysis of SDHB using anti-SDHB antibody (A01090). <br>SDHB was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (A01090) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-ihc-testing-4_1.jpgAnti-SDHB Antibody Picoband®IHC analysis of SDHB using anti-SDHB antibody (A01090). <br>SDHB was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (A01090) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-ihc-testing-5.jpgAnti-SDHB Antibody Picoband®IHC analysis of SDHB using anti-SDHB antibody (A01090). <br>SDHB was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (A01090) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01090-sdhb-primary-antibodies-fcm-testing-1.jpgAnti-SDHB Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-SDHB antibody (A01090). <br>
Overlay histogram showing A431 cells stained with A01090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHB Antibody (A01090, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-splicing-factor-1-picoband-trade-antibody-a01009-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01009-splicing_factor_1-primary-antibodies-wb-testing-1.jpgAnti-splicing factor 1/SF1 Antibody Picoband® Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SK-OV-3 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: mouse NIH/3T3 whole cell lysates, <br>
Lane 4: rat PC-12 whole cell lysates, <br>
Lane 5: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-splicing factor 1 antigen affinity purified polyclonal antibody (Catalog # A01009) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for splicing factor 1 at approximately 68-80 kDa. The expected band size for splicing factor 1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-2.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-3.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-4.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-5.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-6.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01009-splicing_factor_1-primary-antibodies-IHC-testing-7.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009).
<br>splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01009-splicing_factor_1-primary-antibodies-if-testing-8.jpgAnti-splicing factor 1/SF1 Antibody Picoband® IF analysis of splicing factor 1 using anti-splicing factor 1 antibody (A01009). <br>
splicing factor 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-splicing factor 1 Antibody (A01009) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01009-splicing_factor_1-primary-antibodies-fcm-testing-9.jpgAnti-splicing factor 1/SF1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-splicing factor 1 antibody (A01009). <br>Overlay histogram showing A431 cells stained with A01009 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-splicing factor 1 Antibody (A01009, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sftpb-picoband-trade-antibody-a03441-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03441-1-SFTPB-primary-antibodies-WB-testing-1.jpgAnti-Prosurfactant Protein B/SFTPB Antibody Picoband® Western blot analysis of SFTPB using anti-SFTPB antibody (A03441-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human MCF-7 whole cell lysates,<br>Lane 2: human COLO-320 whole cell lysates,<br>Lane 3: human HepG2 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPB antigen affinity purified polyclonal antibody (Catalog # A03441-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPB at approximately 46KD. The expected band size for SFTPB is at 42KD.
https://www.bosterbio.com/products/primary-antibodies/anti-skp2-picoband-trade-antibody-a00544-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00544-skp2-primary-antibodies-wb-testing-1.jpgAnti-SKP2 Antibody Picoband®Western blot analysis of SKP2 using anti-SKP2 antibody (A00544). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human MCF-7 whole cell lysates, <br>
Lane 5: rat testis tissue lysates, <br>
Lane 6: rat C6 whole cell lysates, <br>
Lane 7: mouse testis tissue lysates, <br>
Lane 8: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (A00544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 46, 48 kDa. The expected band size for SKP2 is at 46, 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00544-skp2-primary-antibodies-ihc-testing-1.jpgAnti-SKP2 Antibody Picoband®IHC analysis of SKP2 using anti-SKP2 antibody (A00544). <br>SKP2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKP2 Antibody (A00544) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00544-skp2-primary-antibodies-if-testing-1.jpgAnti-SKP2 Antibody Picoband®IF analysis of SKP2 using anti-SKP2 antibody (A00544) and anti-Tubulin Alpha antibody (M03989-3).<br>
SKP2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SKP2 Antibody (A00544) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-smc6l1-picoband-trade-antibody-a01554-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01554-1-SMC6L1-primary-antibodies-WB-testing-1.jpgAnti-SMC6L1 Antibody Picoband® Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human HepG2 whole cell lysates,<br>Lane 3: human PANC-1 whole cell lysates,<br>Lane 4: human SK-OV-3 whole cell lysates,<br>Lane 5: human COLO-320 whole cell lysates,<br>Lane 6: rat testis tissue lysates,<br>Lane 7: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC6L1 at approximately 126KD. The expected band size for SMC6L1 is at 126KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-1-smc6l1-primary-antibodies-ihc-testing-2.jpgAnti-SMC6L1 Antibody Picoband® IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). <br> SMC6L1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-1-smc6l1-primary-antibodies-ihc-testing-3.jpgAnti-SMC6L1 Antibody Picoband® IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). <br> SMC6L1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-1-smc6l1-primary-antibodies-ihc-testing-4.jpgAnti-SMC6L1 Antibody Picoband® IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). <br> SMC6L1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-1-smc6l1-primary-antibodies-fc-testing-5.pngAnti-SMC6L1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SMC6L1 antibody (A01554-1).<br> Overlay histogram showing A431 cells stained with A01554-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC6L1 Antibody A01554-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-1-smc6l1-primary-antibodies-if-testing-6.jpgAnti-SMC6L1 Antibody Picoband® IF analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). <br> SMC6L1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SMC6L1 Antibody (A01554-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-stub1-picoband-trade-antibody-a01236-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01236-1-stub1-primary-antibodies-wb-testing-1.jpgAnti-STUB1 Antibody Picoband® Western blot analysis of STUB1 using anti-STUB1 antibody (A01236-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: rat pancreas tissue lysates, <br>
Lane 6: mouse pancreas tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STUB1 antigen affinity purified polyclonal antibody (Catalog # A01236-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35 kDa. The expected band size for STUB1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01236-1-STUB1-primary-antibodies-IHC-testing-2.jpgAnti-STUB1 Antibody Picoband® IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
<br>STUB1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01236-1-STUB1-primary-antibodies-IHC-testing-3.jpgAnti-STUB1 Antibody Picoband® IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
<br>STUB1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01236-1-STUB1-primary-antibodies-IHC-testing-4.jpgAnti-STUB1 Antibody Picoband® IHC analysis of STUB1 using anti-STUB1 antibody (A01236-1).
<br>STUB1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01236-1-stub1-primary-antibodies-if-testing-5.jpgAnti-STUB1 Antibody Picoband® IF analysis of STUB1 using anti-STUB1 antibody (A01236-1). <br>
STUB1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STUB1 Antibody (A01236-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01236-1-stub1-primary-antibodies-if-testing-6.jpgAnti-STUB1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-STUB1 antibody (A01236-1).<br>Overlay histogram showing A549 cells stained with A01236-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STUB1 Antibody (A01236-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-synaptotagmin-1-picoband-trade-antibody-a02314-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02314-1-synaptotagmin_1-primary-antibodies-wb-testing-1.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody (Catalog # A02314-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02314-1-Synaptotagmin_1-primary-antibodies-IHC-testing-2.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
Synaptotagmin 1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02314-1-Synaptotagmin_1-primary-antibodies-IHC-testing-3.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
Synaptotagmin 1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02314-1-Synaptotagmin_1-primary-antibodies-IHC-testing-4.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
<br>Synaptotagmin 1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02314-1-synaptotagmin_1-primary-antibodies-ihc-testing-5.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
Synaptotagmin 1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02314-1-synaptotagmin_1-primary-antibodies-fc-testing-6.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-Synaptotagmin 1 antibody (A02314-1). <br>Overlay histogram showing A549 cells stained with A02314-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02314-1-synaptotagmin_1-primary-antibodies-fc-testing-7.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Synaptotagmin 1 antibody (A02314-1). <br>Overlay histogram showing PC-3 cells stained with A02314-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02314-1-synaptotagmin_1-primary-antibodies-fc-testing-8.jpgAnti-Synaptotagmin 1/SYT1 Antibody Picoband® Flow Cytometry analysis of LLC cells using anti-Synaptotagmin 1 antibody (A02314-1). <br>Overlay histogram showing LLC cells stained with A02314-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tlr3-picoband-trade-antibody-a00197-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00197-tlr3-primary-antibodies-wb-testing-1.jpgAnti-TLR3 Antibody Picoband® Western blot analysis of TLR3 using anti-TLR3 antibody (A00197). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: mouse Raw264.7 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR3 antigen affinity purified polyclonal antibody (Catalog # A00197) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR3 at approximately 120 kDa. The expected band size for TLR3 is at 104 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-topoisomerase-i-antibody-a00434-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00434-1-topoisomerase_i-primary-antibodies-wb-testing-1_1.jpgAnti-Topoisomerase I/TOP1 Antibody Picoband® Western blot analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates, <br>
Lane 2: human Daudi whole cell lysates, <br>
Lane 3: human MOLT-4 whole cell lysates, <br>
Lane 4: human MCF-7 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Topoisomerase I antigen affinity purified polyclonal antibody (Catalog # A00434-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Topoisomerase I at approximately 100 kDa. The expected band size for Topoisomerase I is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00434-1-topoisomerase_i-primary-antibodies-if-testing-2.jpgAnti-Topoisomerase I/TOP1 Antibody Picoband® IF analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1). <br>
Topoisomerase I was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Topoisomerase I Antibody (A00434-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-tpx2-antibody-a01610-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01610-1-TPX2-primary-antibodies-WB-testing-1.jpgAnti-TPX2 Antibody Picoband® Western blot analysis of TPX2 using anti-TPX2 antibody (A01610-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human COLO-320 whole cell lysates,<br>Lane 3: human U-87MG whole cell lysates,<br>Lane 4: human SGC-7901 whole cell lysates,<br>Lane 5: rat PC-12 whole cell lysates,<br>Lane 6: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody (Catalog # A01610-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPX2 at approximately 86KD, 100KD. The expected band size for TPX2 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-fcell-09-769547-g004.jpgAnti-TPX2 Antibody Picoband®HMGB3 could inhibit cell survival via TPX2 in NB cells. (A) Nine genes significantly co-expressed with HMGB3 in four datasets of NB. (B) Functional enrichment analysis of nine genes. (C) The mRNA expression of nine genes was detected by qRT-PCR in SK-N-SH cells after HMGB3 knockdown. (D) Pearson correlation of HMGB3 and TPX2 in four NB datasets. (E) Protein expression of TPX2 and HMGB3 was detected by western blotting after HMGB3 knockdown. (F) TPX2 reduction was time-dependent with HMGB3 knockdown in SK-N-SH and SK-N-AS cells. * p < 0.05, ** p < 0.01, and *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.769547/full'>34988076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-fcell-09-769547-g005.jpgAnti-TPX2 Antibody Picoband®TPX2 overexpression can reverse the inhibition of cell proliferation caused by HMGB3 knockdown in SK-N-SH cells. (A) Protein expression was detected in sh-HMGB3 SK-N-SH cells after TPX2 over-expression by western blotting. (B–D) Colon formation, Transwell assay, and immunocytochemistry assay for sh-HMGB3 SK-N-SH cells after TPX2 over-expression. Scale bars, 50 μm * p < 0.05, ** p < 0.01, and *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.769547/full'>34988076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-fcell-09-769547-g006.jpgAnti-TPX2 Antibody Picoband®High expression of HMGB3 and TPX2 correlated to inferior NB prognosis in multiple datasets. (A–C) , Overall survival (OS) of NB patients in three independent datasets. (D, E) Progression-free survival (PFS) and Event-free survival (EFS) in NB patients.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.769547/full'>34988076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-fcell-09-769547-g007.jpgAnti-TPX2 Antibody Picoband®HMGB3 and TPX2 are closely associated with the survival of patients with NB in GSE49710. (A) HMGB3 and TPX2 can be independent prognostic factors for overall survival. (B–D) Nomogram, random forest, and ROC curves of HMGB3, TPX2 and clinicopathological characteristics for NB patients.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.769547/full'>34988076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-12935_2020_1454_fig6_html.pngAnti-TPX2 Antibody Picoband®CircPOSTN regulated TPX2 expression via sponging miR-361-5p in glioma cells. a The binding sequences between miR-361-5p and TPX2, and matched mutant sites were shown. b , c Luciferase activity was determined by dual-luciferase reporter assay in LN229 and U251 cells following co-transfection with TPX2 3′UTR-WT or TPX2 3′UTR-MUT and miR-361-5p or miR-NC according to the design. d – g The mRNA and protein expression levels of TPX2 were estimated using RT-qPCR and western blot assays in glioma tissues and cells, along with controls. h , i The correlation analysis between TPX2 and miR-361-5p or circPOSTN was conducted by Pearson’s correlation analysis. j , k The RT-qPCR and western blot assays were recruited to evaluate the expression levels of TPX2 level in LN229 and U251 cells transfected with miR-NC, miR-361-5p, miR-361-5p + pcDNA-NC or miR-361-5p + pcDNA-circPOSTN. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-12935_2020_1454_fig7_html.pngAnti-TPX2 Antibody Picoband®TPX2 regulated proliferation, apoptosis, and aerobic glycolysis in glioma cells. a – l LN229 and U251 cells were introduced with si-NC or si-TPX2. a The transfection efficiency of si-TPX2 was checked with RT-qPCR assay in LN229 and U251 cells. b , c The cell viability of LN229 and U251 cells was determined with MTT assay. d The apoptosis rate of transfected LN229 and U251 cells was represented by flow cytometry assay. e The western blot assay was used to assay the expression levels of Bcl-2 and Bax in LN229 and U251 cells. f The activity of caspase-3 was detected with a caspase-3 assay kit. g – i The glucose, lactate, and ATP production levels were shown. j The protein expression levels of HK2 and LDHA were estimated by western blot assay in LN229 and U251 cells. k , l LDHA enzyme activity and ROS content were evaluated in LN229 and U251 cells post-transfection. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01610-1-12935_2020_1454_fig9_html.pngAnti-TPX2 Antibody Picoband®Silencing of circPOSTN repressed glioma tumor growth in vivo. a , b Tumor volume and weight were presented. c , d The expression levels of circPOSTN and miR-361-5p in dissected tumor tissues were estimated with RT-qPCR assay. e Western blot assay was executed to test protein level of TPX2 in dissected tumor tissues. f The diagrammatic representation of our conclusion was shown. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-trpc4-picoband-trade-antibody-a02037-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02037-1-TRPC4-primary-antibodies-WB-testing-1.jpgAnti-TRPC4 Antibody Picoband® Western blot analysis of TRPC4 using anti-TRPC4 antibody (A02037-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human COLO-320 whole cell lysates,<br>Lane 3: human MCF-7 whole cell lysates,<br>Lane 4: human 293T whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC4 antigen affinity purified polyclonal antibody (Catalog # A02037-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC4 at approximately 150KD. The expected band size for TRPC4 is at 112KD.
https://www.bosterbio.com/products/primary-antibodies/anti-sae2-uba2-picoband-trade-antibody-a03816-2-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03816-2-UBA2-primary-antibodies-WB-testing-1.jpgAnti-SAE2/UBA2 Antibody Picoband® Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human MCF-7 whole cell lysates,<br>Lane 4: human A549 whole cell lysates,<br>Lane 5: human SK-OV-3 whole cell lysates,<br>Lane 6: human 22RV1 whole cell lysates,<br>Lane 7: human A431 whole cell lysates,<br>Lane 8: human COLO-320 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody (Catalog # A03816-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-fc-testing-7.pngAnti-SAE2/UBA2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SAE2/UBA2 antibody (A03816-2). <br> Overlay histogram showing A431 cells stained with A03816-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAE2/UBA2 Antibody (A03816-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-ihc-testing-2.jpgAnti-SAE2/UBA2 Antibody Picoband® IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).
<br>SAE2/UBA2 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAE2/UBA2 Antibody (A03816-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-ihc-testing-3.jpgAnti-SAE2/UBA2 Antibody Picoband® IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).
<br>SAE2/UBA2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAE2/UBA2 Antibody (A03816-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-ihc-testing-4.jpgAnti-SAE2/UBA2 Antibody Picoband® IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).
<br>SAE2/UBA2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAE2/UBA2 Antibody (A03816-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-ihc-testing-5.jpgAnti-SAE2/UBA2 Antibody Picoband® IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).
<br>SAE2/UBA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAE2/UBA2 Antibody (A03816-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03816-2-uba2-primary-antibodies-if-testing-6.jpgAnti-SAE2/UBA2 Antibody Picoband® IF analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (A03816-2).<br> SAE2/UBA2 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SAE2/UBA2 Antibody (A03816-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-vcam1-picoband-trade-antibody-a01199-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-1-vcam1-primary-antibodies-ihc-testing-1.jpgAnti-VCAM1 Antibody Picoband®IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1). <br>VCAM1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-1-vcam1-primary-antibodies-ihc-testing-2.jpgAnti-VCAM1 Antibody Picoband®IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1). <br>VCAM1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-1-vcam1-primary-antibodies-elisa-testing-2.jpgAnti-VCAM1 Antibody Picoband® Sandwich ELISA - Recombinant human VCAM1 protein standard curve.<br>
Use in combination with reagents from Human VCAM1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0537).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-1-vcam1-primary-antibodies-ihc-testing-3.jpgAnti-VCAM1 Antibody Picoband®IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1). <br>VCAM1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-1-vcam1-primary-antibodies-ihc-testing-4.jpgAnti-VCAM1 Antibody Picoband®IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).<br>
VCAM1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 9.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-VCAM1 Antibody (A01199-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-vcam1-picoband-trade-antibody-a01199-2-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-2-vcam1-primary-antibodies-wb-testing-1_1.jpgAnti-VCAM1 Antibody Picoband® Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat thymus tissue lysates,<br>Lane 3: rat testis tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: rat lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (Catalog # A01199-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 110KD. The expected band size for VCAM1 is at 81KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-2-fphar-11-568585-g006.jpgAnti-VCAM1 Antibody Picoband®Effect of Wosi and colchicine treatment on the level of (A) COX-2 and (C) VCAM-1 proteins were detected by immunohistochemistry (IHC). Representative photomicrographs of staining (×20) demonstrating the synovial tissue sections from ankle joints of experiments rats. Treatment of groups are as follow: (a) control group; (b) model group; (c) colchicine group (0.2 mg/kg); (d) Wosi group (0.5 g/kg); (e) Wosi group (1 g/kg); (f) Wosi group (2 g/kg). The integrated optical density (IOD) of COX-2 (B) and VCAM-1 (D) IHC-stained materials were analyzed utilizing the Image pro plus 7.0 software. IOD = optical intensity of positive cells × area of positive cells. The value was expressed as mean ± SEM for ten animals. ###p < 0.001 vs. control group; **p < 0.01; ***p < 0.001 vs. model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.568585/full'>33442381</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01199-2-VCAM1-primary-antibodies-IHC-testing-2.jpgAnti-VCAM1 Antibody Picoband® IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
<br>VCAM1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01199-2-VCAM1-primary-antibodies-IHC-testing-3.jpgAnti-VCAM1 Antibody Picoband® IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
<br>VCAM1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-2-vcam1-primary-antibodies-ihc-testing-4_1.jpgAnti-VCAM1 Antibody Picoband® IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2). <br> VCAM1 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-vegfb-picoband-trade-antibody-a04494-3-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04494-3-vegfb-primary-antibodies-wb-testing-1.jpgAnti-VEGFB Antibody Picoband® Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human 22RV1 whole cell lysates,<br>Lane 2: rat skeletal muscle tissue lysates,<br>Lane 3: mouse HEPA1-6 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # A04494-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.
https://www.bosterbio.com/products/primary-antibodies/anti-bestrophin-picoband-trade-antibody-a01434-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01407-2-best1-primary-antibodies-wb-testing-1.jpgAnti-Bestrophin/BEST1 Antibody Picoband®Western blot analysis of BEST1 using anti-BEST1 antibody (A01407-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human Y79 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BEST1 antigen affinity purified polyclonal antibody (A01407-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BEST1 at approximately 68 kDa. The expected band size for BEST1 is at 68 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-bikunin-ambp-picoband-trade-antibody-a02419-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02419-1-Alpha_1_microglobulin-primary-antibodies-WB-testing-1.jpgAnti-Bikunin/AMBP Antibody Picoband® Western blot analysis of Bikunin/AMBP using anti-Bikunin/AMBP antibody (A02419-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates,<br>Lane 2: mouse liver tissue lysates,<br>Lane 3: mouse NIH3T3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bikunin/AMBP antigen affinity purified polyclonal antibody (Catalog # A02419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bikunin/AMBP at approximately 34KD. The expected band size for Bikunin/AMBP is at 39KD.
https://www.bosterbio.com/products/primary-antibodies/anti-btg2-picoband-trade-antibody-a01461-1-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01461-1-btg2-primary-antibodies-fcm-testing-2_1.jpgAnti-BTG2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-BTG2 antibody (A01461-1). <br>Overlay histogram showing A431 cells stained with A01461-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BTG2 Antibody (A01461-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01461-1-btg2-primary-antibodies-wb-testing-1_1.jpgAnti-BTG2 Antibody Picoband® Western blot analysis of BTG2 using anti-BTG2 antibody (A01461-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human U20S whole cell lysates, <br>
Lane 4: human A549 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody (Catalog # A01461-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BTG2 at approximately 22 kDa. The expected band size for BTG2 is at 17 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-camkii-alpha-antibody-a03241-1-boster.html2026-03-24T05:16:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-1-camk2a-primary-antibodies-wb-testing-1.jpgAnti-CaMKII alpha/CAMK2A Antibody Picoband®Western blot analysis of CAMK2A using anti-CAMK2A antibody (A03241-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMK2A antigen affinity purified polyclonal antibody (A03241-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAMK2A at approximately 54, 60 kDa. The expected band size for CAMK2A is at 54 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-caspase-4-picoband-trade-antibody-a02941-2-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02941-2-Caspase_4-primary-antibodies-WB-testing-1.jpgAnti-Caspase 4/Casp4 Antibody Picoband® Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse liver tissue lysates,<br>Lane 2: mouse testis tissue lysates,<br>Lane 3: mouse thymus tissue lysates,<br>Lane 4: mouse lung tissue lysates,<br>Lane 5: mouse HEPA1-6 whole cell lysates, <br>Lane 6: mouse NIH3T3 whole cell lysates, <br>Lane 7: mouse SP20 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 4 antigen affinity purified polyclonal antibody (Catalog # A02941-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 4 at approximately 43KD. The expected band size for Caspase 4 is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02941-2-caspase_4-primary-antibodies-wb-testing-2.jpgAnti-Caspase 4/Casp4 Antibody Picoband® Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates,<br>Lane 2: rat testis tissue lysates,<br>Lane 3: rat stomach tissue lysates,<br>Lane 4: rat thymus tissue lysates,<br>Lane 5: human COLO-320 whole cell lysates, <br>Lane 6: human HepG2 whole cell lysates, <br>Lane 7: human 22RV1 whole cell lysates, <br>Lane 8: human PANC-1 whole cell lysates, <br>Lane 9: human SGC-7901 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 4 antigen affinity purified polyclonal antibody (Catalog # A02941-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 4 at approximately 43KD. The expected band size for Caspase 4 is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02941-2-caspase_4-primary-antibodies-if-testing-3.jpgAnti-Caspase 4/Casp4 Antibody Picoband® IF analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2). <br> Caspase 4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Caspase 4 Antibody (A02941-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02941-2-caspase_4-primary-antibodies-fc-testing-4.pngAnti-Caspase 4/Casp4 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody (A02941-2). <br> Overlay histogram showing A431 cells stained with A02941-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (A02941-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-caspase-8-picoband-trade-antibody-a00042-1-boster.html2026-03-27T05:07:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-1-caspase_8-primary-antibodies-wb-testing-1.jpgAnti-Caspase 8/CASP8 Antibody Picoband® Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human SGC-7901 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-1-caspase_8-primary-antibodies-wb-testing-2.jpgAnti-Caspase 8/CASP8 Antibody Picoband® Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: mouse thymus tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55-60KD. The expected band size for Caspase 8 is at 55KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cbfb-antibody-picoband-a01007-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01007-1-CBFb-primary-antibodies-WB-testing-1.jpgAnti-CBFb Antibody Picoband® Western blot analysis of CBFb using anti-CBFb antibody (A01007-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SW620 whole cell lysates, <br>Lane 2: rat thymus tissue lysates,<br>Lane 3: mouse thymus tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBFb antigen affinity purified polyclonal antibody (Catalog # A01007-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBFb at approximately 22KD. The expected band size for CBFb is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01007-1-CBFb-primary-antibodies-IHC-testing-2.jpgAnti-CBFb Antibody Picoband® IHC analysis of CBFb using anti-CBFb antibody (A01007-1).
<br>CBFb was detected in paraffin-embedded section of mouse thymus tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBFb Antibody (A01007-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01007-1-CBFb-primary-antibodies-IHC-testing-3.jpgAnti-CBFb Antibody Picoband® IHC analysis of CBFb using anti-CBFb antibody (A01007-1).
<br>CBFb was detected in paraffin-embedded section of rat thymus tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBFb Antibody (A01007-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01007-1-CBFb-primary-antibodies-IHC-testing-4.jpgAnti-CBFb Antibody Picoband® IHC analysis of CBFb using anti-CBFb antibody (A01007-1).
<br>CBFb was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBFb Antibody (A01007-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01007-1-cbfb-primary-antibodies-if-testing-5.jpgAnti-CBFb Antibody Picoband® IF analysis of CBFb using anti-CBFb antibody (A01007-1). <br> CBFb was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CBFb Antibody (A01007-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01007-1-cbfb-primary-antibodies-fc-testing-6.pngAnti-CBFb Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-CBFb antibody (A01007-1). <br> Overlay histogram showing U20S cells stained with A01007-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBFb Antibody (A01007-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cckbr-picoband-trade-antibody-a01677-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01677-1-CCKBR-primary-antibodies-WB-testing-1.jpgAnti-CCKBR Antibody Picoband® Western blot analysis of CCKBR using anti-CCKBR antibody (A01677-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human PANC-1 whole cell lysates, <br>Lane 2: human U-87MG whole cell lysates, <br>Lane 3: human COLO-320 whole cell lysates, <br>Lane 4: human SGC-7901 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCKBR antigen affinity purified polyclonal antibody (Catalog # A01677-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCKBR at approximately 64KD. The expected band size for CCKBR is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01677-1-CCKBR-primary-antibodies-WB-testing-2.jpgAnti-CCKBR Antibody Picoband® Western blot analysis of CCKBR using anti-CCKBR antibody (A01677-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat stomach tissue lysates,<br>Lane 3: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCKBR antigen affinity purified polyclonal antibody (Catalog # A01677-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCKBR at approximately 64KD. The expected band size for CCKBR is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01677-1-cckbr-primary-antibodies-fc-testing-3.jpgAnti-CCKBR Antibody Picoband® Flow Cytometry analysis of LOVO cells using anti-CCKBR antibody (A01677-1). <br>Overlay histogram showing LOVO cells stained with A01677-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CCKBR Antibody (A01677-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ccr3-picoband-trade-antibody-a01748-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01748-1-CCR3-primary-antibodies-WB-testing-1.jpgAnti-CCR3 Antibody Picoband® Western blot analysis of CCR3 using anti-CCR3 antibody (A01748-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Jurkat whole cell lysates, <br>Lane 2: human HepG2 whole cell lysates, <br>Lane 3: human MCF-7 whole cell lysates, <br>Lane 4: human U-87MG whole cell lysates, <br>Lane 5: human CCRF-CEM whole cell lysates, <br>Lane 6: rat brain tissue lysates,<br>Lane 7: mouse brain tissue lysates,<br>Lane 8: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR3 antigen affinity purified polyclonal antibody (Catalog # A01748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR3 at approximately 55KD. The expected band size for CCR3 is at 41KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01748-1-ccr3-primary-antibodies-fc-testing-2_1.jpgAnti-CCR3 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-CCR3 antibody (A01748-1).<br>Overlay histogram showing RAW264.7 cells stained with A01748-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR3 Antibody (A01748-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cct7-picoband-trade-antibody-a08169-2-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08169-2-cct7-primary-antibodies-wb-testing-1.jpgAnti-TCP1 eta/CCT7 Antibody Picoband® Western blot analysis of CCT7 using anti-CCT7 antibody (A08169-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: human MCF-7 whole cell lysates.<br>
Lane 6: human HEL whole cell lysates,<br>
Lane 7: human U251 whole cell lysates,<br>
Lane 8: human K562 whole cell lysates,<br>
Lane 9: rat C6 whole cell lysates,<br>
Lane 10: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT7 antigen affinity purified polyclonal antibody (Catalog # A08169-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT7 at approximately 59 kDa. The expected band size for CCT7 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08169-2-tcp1_eta-primary-antibodies-fc-testing-2.pngAnti-TCP1 eta/CCT7 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). <br> Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08169-2-tcp1_eta-primary-antibodies-if-testing-3.jpgAnti-TCP1 eta/CCT7 Antibody Picoband® IF analysis of CCT7 using anti-CCT7 antibody (A08169-2). <br> CCT7 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT7 Antibody (A08169-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd80-antibody-a00196-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-1-cd80-primary-antibodies-wb-testing-1_1.jpgAnti-CD80 Antibody Picoband®Western blot analysis of CD80 using anti-CD80 antibody (A00196-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates,<br>
Lane 2: human Daudi whole cell lysates,<br>
Lane 3: human Ramos whole cell lysates,<br>
Lane 4: mouse thymus tissue lysates,<br>
Lane 5: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # A00196-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD80 at approximately 33-70 kDa. The expected band size for CD80 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-1-cd80-primary-antibodies-ihc-testing-1.jpgAnti-CD80 Antibody Picoband®IHC analysis of CD80 using anti-CD80 antibody (A00196-1). <br>
CD80 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD80 Antibody (A00196-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-1-cd80-primary-antibodies-ihc-testing-2_1.jpgAnti-CD80 Antibody Picoband®IHC analysis of CD80 using anti-CD80 antibody (A00196-1). <br>
CD80 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD80 Antibody (A00196-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-1-cd80-primary-antibodies-ihc-testing-3_1.jpgAnti-CD80 Antibody Picoband®IHC analysis of CD80 using anti-CD80 antibody (A00196-1). <br>
CD80 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD80 Antibody (A00196-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd163-picoband-trade-antibody-a00812-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-wb-testing-1.jpgAnti-CD163 Antibody Picoband® Western blot analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCCT tissue lysates, <br>
Lane 2: human HCCP tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # A00812-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cmar-10-4217fig3.jpgAnti-CD163 Antibody Picoband®BAY11-7082 inhibits IL-17-induced expression of CD163. Notes: ( A ) Immunofluorescence assays showed that IL-17 (100 ng/mL for 48 hours) significantly elevated the expression of CD163 in THP-1-derived macrophages. However, the expression of CD163 was decreased if the NF-κB activity was blocked by BAY11-7082. Scale bar represents 0.1 mm. ( B ) Bar graph depicts the relative fluorescence intensity of CD163. The data shown represent mean±SD (n=3 per group, * P <0.05 and *** P <0.001). Abbreviations: DAPI, 40,6-diamidino-2-phenylindole; IL, interleukin; NF-κB, nuclear factor kappa B.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6177522/&orig_host=www.ncbi.nlm.nih.gov'>30323677</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cmar-10-4217fig4.jpgAnti-CD163 Antibody Picoband®IL-17 induces CD203 and CD163 expression in peritoneal macrophages via p65. Notes: ( A ) F4/80 and CD11b antibodies were used to authenticate by flow cytometry. ( B and C ) qRT-PCR and immunoreactive bands for p65 in peritoneal macrophages treated with siRNA-p65. ( D ) Flow cytometry analysis of CD206 expression in IL-17-treated macrophages transfected with siRNA-p65. Numerical values denote the mean fluorescence intensity. The red line is the fluorescence intensity of isotype control. The blue line is the fluorescence intensity of CD206. ( E ) Immunofluorescence assay of CD163. Scale bar represents 0.1 mm. ( F ) Bar graph depicts the relative fluorescence intensity of CD163. The data shown represent mean±SD (n=3 per group, *** P <0.001). ( G ) mRNA levels of Arginase I, Fizz1 and Ym1 were examined in peritoneal macrophages by real-time PCR. The data shown represent mean±SD (n=3 per group, *** P <0.001). Abbreviations: DAPI, 40,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; qRT-PCR, quantitative real-time PCR; TGF-β, transforming growth factor beta.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6177522/&orig_host=www.ncbi.nlm.nih.gov'>30323677</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cmar-10-4217fig5.jpgAnti-CD163 Antibody Picoband®CD163 and c-Maf expression positively correlates with IL-17+ cell and predicts poor survival of NSCLC patients. Notes: ( A ) Serial whole tumor sections from 85 NSCLC patients were used, and representative cases of immunohistochemistry staining of IL-17, CD163 and c-Maf are shown (magnification, 200×). Scale bar represents 0.1 mm. ( B ) Significant positive correlations were found between the IL-17 expression and CD163+ c-Maf+ cells. ( C–E ) Kaplan–Meier curves of overall survival analysis between NSCLC patients. Patients with high expression of IL-17, CD163 and c-Maf had significantly poorer overall survival than patients with low expression. ( F , and G ) NSCLC patients were classified into the following four groups: group I, IL-17+ CD163+ c-Maf+; group II, IL-17-CD163-c-Maf–; group III, IL-17+ CD163-c-Maf– and group IV, IL-17+ CD163+ c-Maf– and IL-17+ CD163-c-Maf+. The survival rates in group I were significantly lower than those in group II. P -values were determined by the log-rank test. Abbreviations: IL, interleukin; NSCLC, non-small-cell lung cancer.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6177522/&orig_host=www.ncbi.nlm.nih.gov'>30323677</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-2.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-3.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-4.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-5.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-6.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human rencal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-7.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00812-1-cd163-primary-antibodies-ihc-testing-8.jpgAnti-CD163 Antibody Picoband® IHC analysis of CD163 using anti-CD163 antibody (A00812-1). <br>
CD163 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd200-ox2-picoband-trade-antibody-a01512-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01512-1-CD200-primary-antibodies-WB-testing-1.jpgAnti-CD200/OX2 Antibody Picoband® Western blot analysis of CD200/OX2 using anti-CD200/OX2 antibody (A01512-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: rat brain tissue lysates,<br>Lane 3: mouse brain tissue lysates,<br>Lane 4: mouse SP20 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD200/OX2 antigen affinity purified polyclonal antibody (Catalog # A01512-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD200/OX2 at approximately 45KD. The expected band size for CD200/OX2 is at 31KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cdc45l-picoband-trade-antibody-a01367-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01367-1-CDC45L-primary-antibodies-WB-testing-1.jpgAnti-CDC45L Antibody Picoband® Western blot analysis of CDC45L using anti-CDC45L antibody (A01367-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysates,<br>Lane 2: rat thymus tissue lysates,<br>Lane 3: rat brain tissue lysates,<br>Lane 4: rat lung tissue lysates,<br>Lane 5: mouse testis tissue lysates,<br>Lane 6: mouse thymus tissue lysates,<br>Lane 7: mouse brain tissue lysates,<br>Lane 8: mouse lung tissue lysates,<br>Lane 9: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC45L antigen affinity purified polyclonal antibody (Catalog # A01367-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC45L at approximately 66KD. The expected band size for CDC45L is at 66KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ve-cadherin-picoband-trade-antibody-a02632-2-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-12951_2025_3343_fig4_html.pngAnti-VE-Cadherin Cdh5-Antibody Picoband®MXene maintained the angiogenic function of OGD-HUVECs in vitro. A Cell scratch experiments were performed on HUVECs, and measurements were performed at 0, 12, and 24 h. Scale bars: 100 μm. B HUVECs were subjected to an tube formation assay after treatment for 24 h, and the results yielded 6 h of culture. Scale bars: 100 μm. C Quantification and analysis of migration area of HUVECs on 12 h and 24 h. D , E Quantification of the number of junctions and the total branching length among the 6 groups. F–H Western blotting bands and quantification of VEGFA and VE-Cadherin protein expression in MXene-treated HUVECs under OGD conditions. N = 3 per group. NC: high-glucose DMEM. SEM error bars are used. Significance (*): p value < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03343-9'>40197477</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-12-667524-g002.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®PF11 enhances angiogenesis in random skin flaps. (A) Immunofluorescence staining for α-SMA labeled microvessels (scale bar: 20 µm). (B) Histogram showing the percentages of a-SMA positive microvessels in the control and PF11 groups. (C) IHC results showing the Cadherin 5 expression in random skin flaps (scan bar, 50 μm). (D) A histogram exhibiting the integral absorbance of Cadherin 5. (E) Results of western blotting showing the expression of MMP9, VEGF, Cadherin 5, and GAPDH. Cropped blots are shown. (F) Histogram showing optical density values of MMP9, VEGF, and Cadherin5 in the control and PF11 group in the flaps. Values are expressed as means ± SD, n = 6 per Group. * p < 0.05 and ** p < 0.01, vs. control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.667524/full'>33995096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-12-667524-g006.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Inhibition of autophagy abolished the positive effects of PF11 on angiogenesis, apoptosis, oxidative stress in skin flaps. (A,B) Immunofluorescence staining showed the α-SMA positive microvessels in the control, PF11, and PF11 + 3MA groups (scale bar: 20 µm). (C,D) Western blotting results exhibited the expression of angiogenesis-related protein MMP9, VEGF, and Cadherin 5. (E,F) Western blotting results exhibited the expression of oxidative stress-related protein SOD1, HO1, and eNOS in the control, PF11, and PF11 + 3MA groups. (G,H) Western blotting results exhibited the expression of apoptosis-related protein Bax, Bcl-2, and C-caspase3. Values are expressed as means ± SD, n = 6 per group. * p < 0.05 and ** p < 0.01, vs. PF11 group. # p < 0.05 and ## p < 0.01, vs. control group. # p < 0.05 and ## p < 0.01, vs. control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.667524/full'>33995096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fcell-09-643996-g002.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®NLRP3-mediated pyroptosis triggers the necrosis of flaps. (A) Immunofluorescence to evaluate the caspase-1 level was conducted to exhibit the pyroptosis level in the skin flaps (scale bar: 25 mm). (B) The fluorescence intensity of caspase-1 analyzed using ImageJ. (C) Image of the immunofluorescence staining of GSDMD-N in skin flaps (scale bar: 25 mm). (D) The fluorescence intensity for GSDMD-N. (E) Western blotting of pyroptosis related proteins in flap tissue from the control and MCC950 groups. (F) Quantification of pyroptosis related proteins immunoblots. (G) Digital images of flap necrosis from the control and MCC950 groups (scale bar, 1.0 cm). (H) Histogram showing the percentage of viable flap area. (I) Digital images of the inner side of the random flap in each group exhibiting tissue edema (scale bar, 1.0 cm). (J) Quantification of the flap tissue water content. (K) Full field LDBF images of flaps by LDBF (scale bar, 1.0 cm). (L) Quantification of signal intensity of blood flow. (M) Morphologic observation of flap tissue by H&E staining (original magnification, ×200; scale bar, 50 μm). (N) H&E staining to show vessels. (O) The expression of CD34 to mark vessels was evaluated by immunohistochemistry staining (original magnification, ×200; scale bar, 50 μm). (P) Histogram showing the CD34-positive vessel densities (Q) Immunohistochemistry for VEGF on the skin flaps from the control and the MCC950 groups (original magnification, ×200; scale bar, 50 μm). (R) Histograms of optical density values for VEGF in IHC. (S) Western blotting of VEGF and cadherin-5 in flap tissue from the control and MCC950 groups. (T) Quantification of VEGF and cadherin-5 immunoblots. Values represent the mean ± SEM, n = 6 per group. * p < 0.05 and ** p < 0.01, vs. control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.643996/full'>33898433</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fcell-09-643996-g005.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®TFE3 ameliorates ROS-induced lysosomal malfunction and downregulates pyroptosis in random skin flaps. (A,E) Immunofluorescence exhibiting the nuclear translocation of TFE3 (red) (scale bar: 25 mm). (B,F) Percentage of TFE3-positive cells. (C,G) Western blot exhibited cytoplasmic TFE3 and nuclear TFE3. (D,H) Quantification of cytoplasmic and nuclear TFE3 proteins immunoblots. (I) ELISA of 8-OHdG, AOPP, and MDA in flap tissue from WT, TFE3 KI, TFE3 KI + scramble and TFE3 KI + TFE3 shRNA groups. (J,N) Western blotting of the lysosome function-related proteins CTSD, LAMP2, CTSB, and CTSL in total protein and cytoplasmic protein. (K,O) Densitometric analysis of CTSD, LAMP2, CTSB, and CTSL. (L) Immunofluorescence for LAMP2 in skin flaps (scale bar: 25 mm). (M) The fluorescence intensity for LAMP2. (N) Western blotting of VEGF, cadheirn-5, SOD1, and HO-1 in flap tissue from WT, TFE3 KI, and TFE3 KI + scramble and TFE3 KI + TFE3 shRNA groups. (O) Densitometric analysis of VEGF, cadherin-5, SOD1, and HO-1. (P) Western blotting of the pyroptosis related proteins. (Q) Quantification of pyroptosis related proteins immunoblots. Values are shown as the mean ± SEM, n = 6 per group. * p < 0.05 and ** p < 0.01, vs. WT group or area I group. # p < 0.05 and ## p < 0.01, vs. TFE3 KI + TFE3 shRNA group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.643996/full'>33898433</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-11-612932-g002.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®NBP improves angiogenesis in multiterritory perforator flap. (A) and (B) IHC of VEGF and CDH5 in the ischemic flap of the control and NBP-treated rats. (C) and (D) Optical density values of VEGF and CDH5. (E) Western blotting of MMP9, VEGF, and CDH5 in control and NBP-treated groups. (F) Optical density values of MMP9, VEGF, and CDH5 from western blot. Gels were run under similar experimental conditions and cropped edited only for clarity. Values are shown as mean ± SEM, n = 6 per group. * p < 0.05 and ** p < 0.01 vs. control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.612932/full'>33584290</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-11-612932-g006.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Rapamycin reverses effects of NBP on angiogenesis, oxidative stress, and apoptosis in multiterritory perforator flap. (A) Autophagosomes (LC3, red) in cells in the control, NBP, NBP + rapamycin, and rapamycin groups. Nuclei are counterstained with DAPI (blue) (scale bar, 20 μm). (B) Digital images of the control, NBP, NBP + rapamycin, and rapamycin groups on POD 7. (C) LDBF in each group on POD 7. (D) Percentage of survival area on POD 7. (E) Percentage of the signal intensity of blood flow within the flap in each group. (F) LC3 positive cells in each group. (G) Autophagy-related protein expression (LC3, CTSD, Beclin1, and SQSTM1/p62) and angiogenesis-related proteins (VEGF, MMP9, and CDH5). (H) Optical density of LC3, CTSD, Beclin1, SQSTM1/p62, VEGF, MMP9, and CDH5 in each group. (L) Apoptosis-related protein expression (CYC, Bax, and CASP3) and oxidative stress-related protein expression (SOD1, HO1, and eNOS) in each group. (M) Optical density of CYC, Bax, CASP3, SOD1, HO1, and eNOS expressions in each group. Gels were run under similar experimental conditions and edited only for clarity. Values are shown as mean ± SEM, n = 6 per group. * p < 0.05 and ** p < 0.01 vs. control group; # p < 0.05 and ## p < 0.01 vs. NBP group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.612932/full'>33584290</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-ajtr0012-4781-f3.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®HSYA promotes angiogenesis in perforator flaps. On POD7, IHC was used to detect VEGF and CDH5 expression in the SCZs of flaps. Western blotting was used to detect the expression of CDH5, MMP9, and VEGF. A. IHC for VEGF expression in the SCZ of flaps (original magnification, 200 ×; scale, 50 μm). B. The optical density values of VEGF were quantified and analyzed. C. IHC for CDH5 expression in the SCZ of flaps (original magnification, 200 ×; scale, 50 μm). D. The optical density values of CDH5 were quantified and analyzed. E. The expressions of CDH5, MMP9, and VEGF protein in the SCZ of flaps of the control and HSYA groups were evaluated by western blotting. The gels were run under the same experimental conditions, and cropped blots are shown. The original image is in . F-H. Optical density values of CDH5, MMP9, and VEGF were quantified and analyzed. Data are expressed as mean ± SD, n = 6 per group. *P < 0.05 and **P < 0.01 vs. control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7476167/&orig_host=www.ncbi.nlm.nih.gov'>32913550</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-11-00951-g004.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Effect of Sit-induced autophagy on angiogenesis in the perforator flap. (A) Microvessels (red) in SCZ of flaps in the control, Sit, and Sit+3MA groups were estimated by immunofluorescence staining for α-SMA in the dermal layer (scale bar: 20 µm). (B) Histograms representing percentages of α-SMA labeled microvessels in each group. (C) H&E staining exhibiting subcutaneous histology of the flap, showing microvessels in SCZ in the control, Sit, and Sit+3MA groups (original magnification ×200; scan bar, 50 μm). (D) Histogram indicating percentage of mean vessel density in each group. (E) IHC for CD34 positive vessels in the control, Sit, and Sit+3MA groups (original magnification ×200; scale bar, 50 µm). (F) Histogram of the percentage of CD34-positive vessel density in each group. (G, I) IHC for VEGF and Cadherin 5 expression in the flap in the control, Sit, and Sit+3MA groups (original magnification ×200; scale bar, 50 µm). (H, J) The optical density values of VEGF and Cadherin 5 in each group. (K) The expressions of MMP9, VEGF, and Cadherin 5 detected by western blotting in the control, Sit, and Sit+3MA groups. All gels have been run under the same experimental conditions and cropped blots are used here. (L) Histogram of the optical density values of MMP9, VEGF, and Cadherin 5 in each group. *p < 0.05 and **p < 0.01, vs control group; @ p < 0.05 and @@ p < 0.01, vs Sit group. Data are presented as mean ± standard error, n = 6 per group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00951/full'>32670067</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-ijbsv15p0325g002.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®MET promotes angiogenesis in random skin flaps. (A&C) IHC for VEGF and Cadherin 5 expressions in the ischemic flaps of the Control and MET groups (original magnification × 200; scale bar, 50μm). (B&D) The optical density values of VEGF and Cadherin 5 were quantified and analyzed in each group. (E-G) Western blotting for VEGF, MMP9, and Cadherin 5 expressions in the ischemic flaps of the Control and MET groups. The gels have been run under the same experimental conditions, and cropped blots are used here. (H-J) Optical density values of VEGF, MMP9, and Cadherin 5 were quantified and analyzed in each group. Values are shown as means± SEM, n = 6 per group. * p < 0.05 and ** p < 0.01, vs. Control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6367544/&orig_host=www.ncbi.nlm.nih.gov'>30745824</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-ijbsv15p0325g006.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Inhibition of autophagy reverses the effects of MET on angiogenesis, apoptosis and oxidative stress. (A) Autophagosomes (red) in cells in Area II of flaps in the MET and MET+3MA groups by Immunofluorescence staining for LC3II (scale bar, 25μm). (B&D) The expressions of autophagy-related protein VPS34, p62, LC3II, Beclin1, and CTSD; angiogenesis-related protein VEGF, Cadherin 5, and MMP9; oxidative stress-related protein SOD1, HO1, and eNOS and apoptosis-related protein Bax, CYC, and CASP3 in each group, were assessed by Western blotting. The gels have been run under the same experimental conditions, and cropped blots are used here. (C&E) Optical density values of VPS34, p62, LC3II, Beclin1, CTSD, VEGF, Cadherin 5, MMP9, SOD1, HO1, eNOS, Bax, CYC, and CASP3 expressions in the two groups. Values are expressed as means± SEM, n = 6 per group. # p < 0.05 and ## p < 0.01, vs. MET group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6367544/&orig_host=www.ncbi.nlm.nih.gov'>30745824</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-09-01178-g002.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Sal B promotes angiogenesis in ischemic random skin flaps. Rats were killed and the samples in middle part of flap area II were harvested for analysis on post-operative day 7. (A,C) IHC of VEGF and cadherin 5 expression of skin flaps in the Control and Sal B group (original magnification: 200×; scale bar: 50 μm). (B , D) Histograms of optical density values of VEGF and cadherin 5 by IHC, respectively. (E – G) Western blotting of MMP9, VEGF, and cadherin 5 levels of skin flaps in the Control and Sal B group, corrected by GAPDH as internal control. The gels have been run under the same experimental conditions. The original images are available in Supplementary Figure . (H – J) Histograms of optical density values of MMP9, VEGF, and cadherin 5 in the two groups as determined by Western blotting. ∗ p < 0.05 and ∗∗ p < 0.01 vs. the control group. Data are means ± SEM ( n = 6 per group).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.01178/full'>30405410</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-fphar-09-01178-g006.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®Inhibition of autophagy reverses the effects of Sal B in random skin flaps. Rats were killed and the samples in middle part of flap area II were harvested for analysis on post-operative day 7. (A) Autophagy level in skin flaps were estimated by immunofluorescence staining of LC3II:Autophagosomes (green) in the dermis in area II in each group; nuclei counterstained with DAPI (blue) (scale bar: 15 μm). (B) Histogram of frequency of LC3II-positive cells in the dermis. (C) Western blotting of Beclin1, VPS34, CTSD, LC3II, and p62 levels, as well as those of MMP9, VEGF, and cadherin 5, corrected by GAPDH as internal control. The gels have been run under the same experimental conditions. The original images are available in Supplementary Figures . (D) Histograms of optical density values of autophagy- (Beclin1, VPS34, CTSD, LC3II, and p62) and angiogenesis- (MMP9, VEGF, and cadherin 5) related proteins, assessed by Western blotting. (E) Western blotting of Bax, CYC, and caspase 3 levels, as well as those of SOD1, eNOS, and HO1, corrected by GAPDH as internal control. The original images are available in Supplementary Figures . (F) Histograms of optical density values of apoptosis- (Bax, CYC, and caspase 3) and oxidative stress-related proteins (Bax, CYC, and caspase 3) as determined by Western blotting. (G) Total SOD activity by xanthine oxidase method. (H) GSH level by modified 5,5′-dithiobis [2-nitrobenzoic acid] method. (I) MDA level by modified thiobarbituric acid test. ∗ p < 0.05 and ∗∗ p < 0.01 vs. the control group. Data are means ± SEM ( n = 6 per group); NS, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.01178/full'>30405410</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-41598_2018_23568_fig6_html.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband®( a , b ) Effect of exercise on alcohol induced vascular permeability and BBB dysfunction. Representative western blot analysis showing the levels of tight junction (TJ) proteins (ZO-1 and Claudin-5) in the different mice groups ( a ). Histogram showing the quantitative estimation of ZO-1 and Claudin-5 proteins after normalization with GAPDH (b). ( c,d ) Representative images showing fluorescent protein (FITC-BSA) leakage from pial vessels into brain parenchyma – indicating alteration in microvascular permeability in the different groups of mice ( c ). Scatter dot plot showing quantitative estimation of fluorescent intensity units (FIU) in the different mice groups after FITC-BSA injection ( d ). ( e , f ) Representative images of cerebral angiogram with barium sulfate contrast in experimental mice groups ( e ). Scatter dot plot showing the pattern of vascular density in the form of percentage of vascular area in the different mice groups ( f ). ( g , h ) Representative images for the in vitro model showing microvascular permeability in brain endothelial cells (bEnd.3 cells) by FITC-BSA diffusion assay. Fluorescence intensity of bovine serum albumin conjugated with FITC (BSA-488) in lower chambers of Transwells was measured by fluorimetry and presented as FIU ( g ). Histogram showing quantitative estimation of FIU in different experimental conditions after FITC-BSA treatment in Transwell chambers ( h ). ( i , j ) Representative western blot analysis showing the levels of junctional proteins (VE-Cadherin, Claudin-5 and ZO-2) in different experimental conditions of mouse brain endothelial cells (i). Histograms showing the quantitative estimation of ZO-2, Claudin-5 and VE-Cadherin proteins after normalization with GAPDH (j). All the data are represented as mean values ± standard error (SE) in 5 independent experiments. * ,# p < 0.05 considered significant.*p < 0.05 vs. CT and # p < 0.05 vs. AL group. Uncropped blots for Fig. are presented in Supplementary Fig. and . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-23568-z'>29581524</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-cdh5-primary-antibodies-wb-testing-1.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband® Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-cdh5-primary-antibodies-ihc-testing-2.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband® IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). <br>
VE Cadherin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-cdh5-primary-antibodies-ihc-testing-3.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband® IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2). <br>
VE Cadherin was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02632-2-ve_cadherin-primary-antibodies-elisa-testing-4.jpgAnti-VE-Cadherin Cdh5-Antibody Picoband® Sandwich ELISA - Recombinant mouse VE Cadherin/Cdh5 protein standard curve.<br>
Use in combination with reagents from Mouse VE Cadherin/Cdh5 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1318).
https://www.bosterbio.com/products/primary-antibodies/anti-cep68-picoband-trade-antibody-a01704-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01704-1-CEP68-primary-antibodies-WB-testing-1.jpgAnti-CEP68 Antibody Picoband® Western blot analysis of CEP68 using anti-CEP68 antibody (A01704-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human COLO-320 whole cell lysates, <br>Lane 3: human SK-OV-3 whole cell lysates, <br>Lane 4: human Jurkat whole cell lysates, <br>Lane 5: rat heart tissue lysates,<br>Lane 6: mouse heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP68 antigen affinity purified polyclonal antibody (Catalog # A01704-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP68 at approximately 81KD. The expected band size for CEP68 is at 81KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ces1-picoband-trade-antibody-a01741-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01741-1-ces1-primary-antibodies-wb-testing-1.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® Western blot analysis of CES1 using anti-CES1 antibody (A01741-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse HEPA1-6 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # A01741-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CES1 at approximately 62 kDa. The expected band size for CES1 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01741-1-CES1-primary-antibodies-IHC-testing-2.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® IHC analysis of CES1 using anti-CES1 antibody (A01741-1).
<br>CES1 was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CES1 Antibody (A01741-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01741-1-CES1-primary-antibodies-IHC-testing-3.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® IHC analysis of CES1 using anti-CES1 antibody (A01741-1).
<br>CES1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CES1 Antibody (A01741-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01741-1-CES1-primary-antibodies-IHC-testing-4.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® IHC analysis of CES1 using anti-CES1 antibody (A01741-1).
<br>CES1 was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CES1 Antibody (A01741-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01741-1-ces1-primary-antibodies-ihc-testing-5.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® IHC analysis of CES1 using anti-CES1 antibody (A01741-1). <br> CES1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CES1 Antibody (A01741-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01741-1-ces1-primary-antibodies-if-testing-6.jpg.jpgAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® IF analysis of CES1 using anti-CES1 antibody (A01741-1). <br>
CES1 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CES1 Antibody (A01741-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01741-1-ces1-primary-antibodies-fcm-testing-7.jpg.pngAnti-Liver Carboxylesterase 1/CES1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CES1 antibody (A01741-1). <br>Overlay histogram showing HepG2 cells stained with A01741-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CES1 Antibody (A01741-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ckb-picoband-trade-antibody-a01695-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01695-1-CKB-primary-antibodies-WB-testing-1.jpgAnti-Creatine kinase B type/CKB Antibody Picoband® Western blot analysis of CKB using anti-CKB antibody (A01695-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human COLO-320 whole cell lysates, <br>Lane 4: human SW620 whole cell lysates, <br>Lane 5: human MDA-MB-231 whole cell lysates, <br>Lane 6: rat brain tissue lysates,<br>Lane 7: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKB antigen affinity purified polyclonal antibody (Catalog # A01695-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CKB at approximately 45KD. The expected band size for CKB is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01695-1-oncotarget-14-28436-g001.jpgAnti-Creatine kinase B type/CKB Antibody Picoband®Immunohistochemistry of CKB and MTCK in CRCs. ( A , B ) Immunohistochemical examination of CKB (A) and MTCK (B). Immunohistochemical activity is classified into grade 0–3. Grade 0 is equivalent to the expression in the normal colonic epithelium. ( C ) Histogram of expression grade. ( D ) Immunohistochemical examination of CKB and MTCK in case of well-differentiated adenocarcinoma (tub1), signet ring cell carcinoma (sig), primary tumor and liver metastasis of tub1 case. Scale bar, 100 μm. Abbreviations: CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; CRC: colorectal cancer.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10197964/&orig_host=www.ncbi.nlm.nih.gov'>37204253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01695-1-oncotarget-14-28436-g002.jpgAnti-Creatine kinase B type/CKB Antibody Picoband®Creatine kinase inhibitory effect of DNFB. ( A ) Protein fractionation of mitochondria (Mitochondrial) and mitochondria-free cytosol (Cytosol). MTCK and CKB were detected by western blotting. TOM20 and GAPDH were subjected as a mitochondrial marker and a cytosol marker, respectively. ( B ) Inhibition of creatine kinase activity (at 20 min) of CKB (cytosol fraction) and MTCK (mitochondrial fraction) in a DNFB concentration-dependent manner. ( C , D ) Inhibition of creatine kinase activity of CKB (C) and MTCK (D) in a time-dependent manner. Error bar, standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; DNFB: dinitrofluorobenzene; TOM20: translocase of the outer membrane 20; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10197964/&orig_host=www.ncbi.nlm.nih.gov'>37204253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01695-1-oncotarget-14-28436-g003.jpgAnti-Creatine kinase B type/CKB Antibody Picoband®Effect of creatine shuttle inhibition by DNFB on growth and stemness of CRC cells. ( A ) Effect of DNFB on cell growth. ( B ) Effect of pCr on DNFB-treated CRC cells. ( C ) Effect of DNFB (10 μM) on expression of stemness-associated genes detected by quantitative RT-PCR. β-Actin was used as a loading control. ( D ) Effect of DNFB (10 μM) on sphere formation. Scale bar, 50 μm. ( E , F ) Effect of knockdown of CKB or MTCK on cell proliferation (E) and sphere formation (F). Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; DNFB: dinitrofluorobenzene; CRC: colorectal cancer; OD: optimal density; pCr: phosphocreatine; C: untreated control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10197964/&orig_host=www.ncbi.nlm.nih.gov'>37204253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01695-1-oncotarget-14-28436-g007.jpgAnti-Creatine kinase B type/CKB Antibody Picoband®Creatine shuttle provides ATP for EGFR phosphorylation in EGF-treated HT29 cells. ( A ) Effect of DNFB (5 μM) and knockdown of MTCK or CKB on EGFR phosphorylation. Lower panels, effect of siMTCK and siCKB on expression of MTCK and CKB, respectively. ( B ) Semi-quantification of EGFR phosphorylation levels. ( C ) Effect of ATP on suppressed EGFR phosphorylation by DNFB (5 μM). ( D ) Effect of oligomycin on EGFR phosphorylation. ( E ) Effect of CyCr on creatine phosphorylation. ( F ) Effect of CyCr on EGFR phosphorylation. ( G ) Co-immunoprecipitation using anti-CKB antibody or anti-EGFR antibody to examine binding of CKB and EGFR. ( H ) Duolink ® proximity ligation assay. Red, proximity signal of EGFR and CKB. Blue, DAPI. Scale bar, 10 μm. Error bars: standard deviation of three independent trials. Statistical significance was calculated using a two-tailed ordinary analysis of variance. Abbreviations: DNFB: dinitrofluorobenzene; EGFR: epithelial growth factor receptor; pEGFR: phosphorylated EGFR; EGF: epithelial growth factor; CKB: creatine kinase B; MTCK: mitochondrial creatine kinase; siC: short interfering RNA (siRNA) used as control; siMTCK: siRNA for MTCK; siCKB: siRNA for CKB; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; OM: oligomycin; CyCr: cyclocreatine; pCr: phosphocreatine; IP: immunoprecipitation; CB: Coomassie blue; WCL: whole cell lysate; DAPI: 4′,6-diamidino-2-phenylindole.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10197964/&orig_host=www.ncbi.nlm.nih.gov'>37204253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01695-1-CKB-primary-antibodies-IHC-testing-2.jpgAnti-Creatine kinase B type/CKB Antibody Picoband® IHC analysis of CKB using anti-CKB antibody (A01695-1).
<br>CKB was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CKB Antibody (A01695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01695-1-CKB-primary-antibodies-IHC-testing-3.jpgAnti-Creatine kinase B type/CKB Antibody Picoband® IHC analysis of CKB using anti-CKB antibody (A01695-1).
<br>CKB was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CKB Antibody (A01695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01695-1-CKB-primary-antibodies-IHC-testing-4.jpgAnti-Creatine kinase B type/CKB Antibody Picoband® IHC analysis of CKB using anti-CKB antibody (A01695-1).
<br>CKB was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CKB Antibody (A01695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-galectin-10-picoband-trade-antibody-a01350-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01350-Galectin_10-primary-antibodies-WB-testing-1.jpgAnti-Galectin 10/CLC Antibody Picoband® Western blot analysis of Galectin 10 using anti-Galectin 10 antibody (A01350).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HL-60 whole cell lysates, <br>Lane 2: human PANC-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 10 antigen affinity purified polyclonal antibody (Catalog # A01350) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 10 at approximately 16KD. The expected band size for Galectin 10 is at 16KD.
https://www.bosterbio.com/products/primary-antibodies/anti-clpp-picoband-trade-antibody-a01117-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-wb-testing-1.jpgAnti-CLPP Antibody Picoband® Western blot analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: rat pancreas tissue lysates, <br>
Lane 6: rat lung tissue lysates, <br>
Lane 7: mouse pancreas tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPP antigen affinity purified polyclonal antibody (Catalog # A01117-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPP at approximately 26KD. The expected band size for CLPP is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-ihc-testing-2.jpgAnti-CLPP Antibody Picoband® IHC analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-ihc-testing-3.jpgAnti-CLPP Antibody Picoband® IHC analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-ihc-testing-4.jpgAnti-CLPP Antibody Picoband® IHC analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-ihc-testing-5.jpgAnti-CLPP Antibody Picoband® IHC analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-ihc-testing-6.jpgAnti-CLPP Antibody Picoband® IHC analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-if-testing-7.jpgAnti-CLPP Antibody Picoband® IF analysis of CLPP using anti-CLPP antibody (A01117-1). <br>
CLPP was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CLPP Antibody (A01117-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01117-1-clpp-primary-antibodies-fcm-testing-8.pngAnti-CLPP Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CLPP antibody (A01117-1). <br>Overlay histogram showing U937 cells stained with A01117-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLPP Antibody (A01117-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cannabinoid-receptor-i-antibody-a01291-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01291-1-Cannabinoid_Receptor_I-primary-antibodies-WB-testing-1.jpgAnti-Cannabinoid Receptor I/CNR1 Antibody Picoband® Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human 22RV1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody (Catalog # A01291-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01291-1-Cannabinoid_Receptor_I-primary-antibodies-IHC-testing-2.jpgAnti-Cannabinoid Receptor I/CNR1 Antibody Picoband® IHC analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
<br>Cannabinoid Receptor I was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cannabinoid Receptor I Antibody (A01291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01291-1-Cannabinoid_Receptor_I-primary-antibodies-IHC-testing-3.jpgAnti-Cannabinoid Receptor I/CNR1 Antibody Picoband® IHC analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
<br>Cannabinoid Receptor I was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cannabinoid Receptor I Antibody (A01291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01291-1-cnr1-primary-antibodies-fc-testing-4.jpgAnti-Cannabinoid Receptor I/CNR1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-CNR1 antibody (A01291-1).<br>Overlay histogram showing Hela cells stained with A01291-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CNR1 Antibody (A01291-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-torc2-picoband-trade-antibody-a01118-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01118-1-TORC2-primary-antibodies-WB-testing-1.jpgAnti-TORC2/CRTC2 Antibody Picoband® Western blot analysis of TORC2 using anti-TORC2 antibody (A01118-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human MCF-7 whole cell lysates, <br>Lane 3: human A375 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC2 antigen affinity purified polyclonal antibody (Catalog # A01118-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC2 at approximately 73KD. The expected band size for TORC2 is at 73KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cullin-4a-picoband-trade-antibody-a01579-1-boster.html2026-03-24T05:16:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01579-1-Cullin_4a-primary-antibodies-WB-testing-1.jpgAnti-Cullin 4a/CUL4A Antibody Picoband® Western blot analysis of Cullin 4a using anti-Cullin 4a antibody (A01579-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human COLO-320 whole cell lysates, <br>Lane 3: human MDA-MB-231 whole cell lysates, <br>Lane 4: human Jurkat whole cell lysates, <br>Lane 5: human PANC-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4a antigen affinity purified polyclonal antibody (Catalog # A01579-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4a at approximately 88KD. The expected band size for Cullin 4a is at 88KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01579-1-Cullin_4a-primary-antibodies-WB-testing-2.jpgAnti-Cullin 4a/CUL4A Antibody Picoband® Western blot analysis of Cullin 4a using anti-Cullin 4a antibody (A01579-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat spleen tissue lysates,<br>Lane 3: rat heart tissue lysates,<br>Lane 4: rat testis tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse spleen tissue lysates,<br>Lane 7: mouse heart tissue lysates,<br>Lane 8: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4a antigen affinity purified polyclonal antibody (Catalog # A01579-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4a at approximately 88KD. The expected band size for Cullin 4a is at 88KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cullin-5-picoband-trade-antibody-a01925-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01925-1-Cullin_5-primary-antibodies-WB-testing-1.jpgAnti-Cullin 5/CUL5 Antibody Picoband® Western blot analysis of Cullin 5 using anti-Cullin 5 antibody (A01925-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat spleen tissue lysates,<br>Lane 3: rat heart tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse spleen tissue lysates,<br>Lane 7: mouse heart tissue lysates,<br>Lane 8: mouse kidney tissue lysates,<br>Lane 9: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 5 antigen affinity purified polyclonal antibody (Catalog # A01925-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 5 at approximately 91KD. The expected band size for Cullin 5 is at 91KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dynamin-2-picoband-trade-antibody-a01629-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01629-1-Dynamin_2-primary-antibodies-WB-testing-1.jpgAnti-Dynamin 2/DNM2 Antibody Picoband® Western blot analysis of Dynamin 2 using anti-Dynamin 2 antibody (A01629-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 2 antigen affinity purified polyclonal antibody (Catalog # A01629-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dynamin 2 at approximately 100KD. The expected band size for Dynamin 2 is at 98KD.
https://www.bosterbio.com/products/primary-antibodies/anti-desmoplakin-picoband-trade-antibody-a00616-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00616-1-desmoplakin-primary-antibodies-wb-testing-1.jpgAnti-Desmoplakin/DSP Antibody Picoband® Western blot analysis of Desmoplakin using anti-Desmoplakin antibody (A00616-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin antigen affinity purified polyclonal antibody (Catalog # A00616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoplakin at approximately 250-280KD. The expected band size for Desmoplakin is at 332KD.
https://www.bosterbio.com/products/primary-antibodies/anti-egr1-picoband-trade-antibody-a00687-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00687-1-egr1-primary-antibodies-wb-testing-1.jpgAnti-Egr1 Antibody Picoband® Western blot analysis of Egr1 using anti-Egr1 antibody (A00687-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human 22RV1 whole cell lysates, <br>Lane 2: human PANC-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified polyclonal antibody (Catalog # A00687-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Egr1 at approximately 75KD. The expected band size for Egr1 is at 58KD.
https://www.bosterbio.com/products/primary-antibodies/anti-tbr2-eomes-picoband-trade-antibody-a00992-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00992-1-TBR2-primary-antibodies-WB-testing-1.jpgAnti-TBR2/Eomes Antibody Picoband® Western blot analysis of TBR2/Eomes using anti-TBR2/Eomes antibody (A00992-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat thymus tissue lysates,<br>Lane 3: mouse spleen tissue lysates,<br>Lane 4: mouse thymus tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBR2/Eomes antigen affinity purified polyclonal antibody (Catalog # A00992-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBR2/Eomes at approximately 85KD. The expected band size for TBR2/Eomes is at 73KD.
https://www.bosterbio.com/products/primary-antibodies/anti-epo-picoband-trade-antibody-a00484-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00484-1-EPO-primary-antibodies-WB-testing-1.jpgAnti-EPO Antibody Picoband® Western blot analysis of EPO using anti-EPO antibody (A00484-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse small intestine tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 28KD. The expected band size for EPO is at 21KD.
https://www.bosterbio.com/products/primary-antibodies/anti-eps15-antibody-a01681-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01681-eps15-primary-antibodies-wb-testing-1.jpgAnti-EPS15 Antibody Picoband® Western blot analysis of EPS15 using anti-EPS15 antibody (A01681). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A375 whole cell lysates, <br>Lane 2: human HepG2 whole cell lysates, <br>Lane 3: human Jurkat whole cell lysates, <br>Lane 4: human PANC-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 140KD. The expected band size for EPS15 is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01681-eps15-primary-antibodies-wb-testing-2.jpgAnti-EPS15 Antibody Picoband® Western blot analysis of EPS15 using anti-EPS15 antibody (A01681). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat liver tissue lysates,<br>Lane 3: mouse brain tissue lysates,<br>Lane 4: mouse spleen tissue lysates,<br>Lane 5: mouse liver tissue lysates,<br>Lane 6: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 140KD. The expected band size for EPS15 is at 99KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ire1-picoband-trade-antibody-a00683-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00683-1-IRE1-primary-antibodies-WB-testing-1.jpgAnti-IRE1/ERN1 Antibody Picoband® Western blot analysis of IRE1 using anti-IRE1 antibody (A00683-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human A549 whole cell lysates, <br>Lane 2: human SK-OV-3 whole cell lysates, <br>Lane 3: human PANC-1 whole cell lysates, <br>Lane 4: rat brain tissue lysates,<br>Lane 5: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRE1 antigen affinity purified polyclonal antibody (Catalog # A00683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRE1 at approximately 170KD. The expected band size for IRE1 is at 110KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00683-1-12931_2024_2986_fig2_html.pngAnti-IRE1/ERN1 Antibody Picoband®Over-expression of circSSR1 inhibited vascular smooth muscle cell pyroptosis. A , Over-expression of circSSR1 reduce reactive oxygen species probes (DCFH-DA, green) staining in various groups ( n = 6). B , Endoplasmic reticulum stress (ERS) related protein ATF6, IRE1 and GRP78 expression. C , LDH cytotoxicity assay kit (LDH) was used to detect LDH release ( n = 6). D , Western blot to demonstrate pyroptosis related protein NLRP3, GSDMD and Caspase-1 expression ( n = 5). E , Fluorescence staining of IL-1β in PASMCs under hypoxia ( n = 6). F , Over-expression of circSSR1 plasmid decreased PI positive cells ( n = 5). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-024-02986-w'>39354535</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00683-1-41419_2020_2930_fig3_html.pngAnti-IRE1/ERN1 Antibody Picoband®BCAT1 regulates autophagy through the endoplasmic reticulum stress pathway. a Expression of BCAT1 and ER-Tracker Red staining in PASMCs exposed to NOR or HYP for 24 h. Scale bar = 50 μm ( n = 3). b Western blot analysis of PERK, IRE1, ATF6, and GRP78 protein expression in the ERs pathway in PASMCs treated with gabapentin ( n = 8). c Western blot analysis of IRE1, PERK, ATF6, and GRP78 expression in PASMCs transfected with BCAT1 siRNA ( n = 8). d Western blot analysis of BECN1 and Atg5 in PASMCs treated with the ERs pathway inhibitor 4-PBA and BCAT1 plasmid ( n = 8). e Coimmunoprecipitation of the whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-IRE1, followed by probing with anti-BCAT1 ( n = 3). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, N + Con normoxia plus control vector, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+4 hypoxia plus control vector plus 4-phenylbutyric acid, H + B + 4 hypoxia plus BCAT1 plasmid plus 4-phenylbutyric acid, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00683-1-41467_2024_52617_fig6_html.pngAnti-IRE1/ERN1 Antibody Picoband®Calcium flux in CD4 + CD38 + T cells leads to ER stress in T cells. A Scatter plot of flow cytometry analysis of IL-2 comparing CD4 + CD38 - and CD4 + CD38 + T cells from patients with SLE ( n = 10, left panel) and from healthy participants ( n = 6, middle panel) and representative dot plot of IL-2 production in healthy participants and SLE patients in CD38 - and CD38 + in CD4 + T cells (right panel). CD4 + T cells were stimulated with PMA/Ionomycin for 6 h with brefeldin and intracellular staining for IL-2 was performed. B Cumulative data (left panel) and representative plots (right panel) of flow cytometry analysis of IL-2 positive CD4 + CD38 + T cells from patients with SLE ( n = 3) and from healthy participants ( n = 4). CD4 + T cells were stimulated with PMA/Ionomycin for 6 h with brefeldin and intracellular staining for IL-2 was performed. C Cumulative data of flow cytometry analysis of IL-2 production in total CD4 + T cells comparing healthy participants ( n = 6) and patients with SLE ( n = 5). Cells were stimulated with PMA/Ionomycin for 5 h and intracellular staining for IL-2 was performed. D Pathway analysis of RNA-seq data comparing Jurkat Control and Jurkat CD38KO cells ( n = 3 from each group). Bar graphs represent GO Cellular Component 2018 pathway that was upregulated in Jurkat Control compared to Jurkat CD38KO cells with adjusted p values < 0.05 and Fold Change > 1.5 (2-sided tests). E Gene set enrichment plot of the genes upregulated in response to ER stress calculated by GSEA (gene set from GO:1990440 was used). F Flow cytometry analysis of IRE1 comparing CD4 + CD38 - and CD4 + CD38 + T cells from healthy participants ( n = 6, left panel) and from patients with SLE ( n = 6, right panel). G Flow cytometry analysis of spliced XBP1 comparing CD4 + CD38 - and CD4 + CD38 + T cells from healthy participants ( n = 5, left panel) and from patients with SLE ( n = 5, middle panel) and representative dot plot (right panel). H Representative electron microscope images of ER morphology in CD4 + CD38 - and CD4 + CD38 + T cells from patients with SLE and healthy participants. The arrow points to a normal strand of ER in a CD4 + CD38 - cell of a patient with SLE and the star marks point to dilated strands of ER in the cytoplasm and adjacent to the nuclear membrane (inset) in a CD4 + CD38 + cell from another patient with SLE. Cells from healthy individuals (low panels) show no ER changes. 3 independent experiments were performed. Scale bars, 500 nm. Data analysis using unpaired 2-tailed Student’s t test with Welch’s correction ( B , C ) or paired 2-tailed Student’s t test ( A , F , G ). All data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-52617-7'>39333474</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00683-1-41419_2020_2930_fig4_html.pngAnti-IRE1/ERN1 Antibody Picoband®BCAT1 regulates autophagy during hypoxia by activating ERs via the IRE1-XBP1-RIDD axis. a Western blot analysis of BECN1 and Atg5 in PASMCs cotransfected with BCAT1 and IRE1 siRNA ( n = 5). b Autophagic flux was monitored in PASMCs cotransfected with eGFP-mRFP-LC3 plasmid and control siRNA or IRE1 siRNA that were then exposed to HYP for 24 h. Scale bar = 50 μm ( n = 3). c , d RT-PCR analysis of the mRNA levels of XBP1-s, sparc, pmp2, and Scara3 with rat β-actin serving as the standard ( n = 5). e The formation of autophagosomes was detected, and autophagic activity was estimated in cells in which the expression of XBP1 was knocked down with XBP1 siRNA under HYP for 24 h. Scale bar = 50 µm ( n = 5). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + SI-IRE1 hypoxia plus IRE1 siRNA, H + SI-XBP1 hypoxia plus XBP1 siRNA, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+NC hypoxia plus control vector plus control siRNA, H + B + Si-IRE hypoxia plus BCAT1 plasmid plus IRE1 siRNA. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-esrrg-picoband-trade-antibody-a01470-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01470-1-ESRRG-primary-antibodies-WB-testing-1.jpgAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® Western blot analysis of ESRRG using anti-ESRRG antibody (A01470-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human PANC-1 whole cell lysates, <br>Lane 2: human 22RV1 whole cell lysates, <br>Lane 3: human COLO-320 whole cell lysates, <br>Lane 4: human SW620 whole cell lysates, <br>Lane 5: mouse SP20 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified polyclonal antibody (Catalog # A01470-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51KD. The expected band size for ESRRG is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01470-1-ESRRG-primary-antibodies-IHC-testing-2.jpgAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1).
<br>ESRRG was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01470-1-ESRRG-primary-antibodies-IHC-testing-3.jpgAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1).
<br>ESRRG was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01470-1-esrrg-primary-antibodies-ihc-testing-4.jpgAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1). <br>
ESRRG was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01470-1-esrrg-primary-antibodies-if-testing-5.pngAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® IF analysis of ESRRG using anti-ESRRG antibody (A01470-1).<br> ESRRG was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01470-1-esrrg-primary-antibodies-fc-testing-6.pngAnti-strogen Related Receptor gamma/ESRRG Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-ESRRG antibody (A01470-1).<br> Overlay histogram showing U20S cells stained with A01470-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ESRRG Antibody (A01470-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gpcr-gpr40-picoband-trade-antibody-a01349-2-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01349-2-GPCR_GPR40-primary-antibodies-WB-testing-1.jpgAnti-GPCR GPR40/FFAR1 Antibody Picoband® Western blot analysis of GPCR GPR40 using anti-GPCR GPR40 antibody (A01349-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human PANC-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR GPR40 antigen affinity purified polyclonal antibody (Catalog # A01349-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR GPR40 at approximately 31KD. The expected band size for GPCR GPR40 is at 31KD.
https://www.bosterbio.com/products/primary-antibodies/anti-gda-picoband-trade-antibody-a01619-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01619-1-GDA-primary-antibodies-WB-testing-1.jpgAnti-GDA Antibody Picoband® Western blot analysis of GDA using anti-GDA antibody (A01619-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat liver tissue lysates,<br>Lane 3: rat small intestine tissue lysates,<br>Lane 4: mouse brain tissue lysates,<br>Lane 5: mouse liver tissue lysates,<br>Lane 6: mouse small intestine tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDA antigen affinity purified polyclonal antibody (Catalog # A01619-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDA at approximately 51KD. The expected band size for GDA is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01619-1-GDA-primary-antibodies-IHC-testing-2.jpgAnti-GDA Antibody Picoband® IHC analysis of GDA using anti-GDA antibody (A01619-1).
<br>GDA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GDA Antibody (A01619-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01619-1-GDA-primary-antibodies-IHC-testing-3.jpgAnti-GDA Antibody Picoband® IHC analysis of GDA using anti-GDA antibody (A01619-1).
<br>GDA was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GDA Antibody (A01619-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01619-1-GDA-primary-antibodies-IHC-testing-4.jpgAnti-GDA Antibody Picoband® IHC analysis of GDA using anti-GDA antibody (A01619-1).
<br>GDA was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GDA Antibody (A01619-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-gfi1-picoband-trade-antibody-a00888-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00888-1-GFI1-primary-antibodies-IHC-testing-1.jpgAnti-GFI1 Antibody IHC analysis of GFI1 using anti-GFI1 antibody (A00888-1).
<br>GFI1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFI1 Antibody (A00888-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00888-1-GFI1-primary-antibodies-IHC-testing-2.jpgAnti-GFI1 Antibody IHC analysis of GFI1 using anti-GFI1 antibody (A00888-1).
<br>GFI1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFI1 Antibody (A00888-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-gns-picoband-trade-antibody-a00999-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00999-1-GNS-primary-antibodies-WB-testing-1.jpgAnti-GNS Antibody Picoband® Western blot analysis of GNS using anti-GNS antibody (A00999-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates, <br>Lane 2: human PANC-1 whole cell lysates, <br>Lane 3: human SGC-7901 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody (Catalog # A00999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00999-1-GNS-primary-antibodies-WB-testing-2.jpgAnti-GNS Antibody Picoband® Western blot analysis of GNS using anti-GNS antibody (A00999-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat lung tissue lysates,<br>Lane 2: rat kidney tissue lysates,<br>Lane 3: rat testis tissue lysates,<br>Lane 4: rat PC-12 whole cell lysates, <br>Lane 5: mouse lung tissue lysates,<br>Lane 6: mouse kidney tissue lysates,<br>Lane 7: mouse testis tissue lysates,<br>Lane 8: mouse spleen tissue lysates,<br>Lane 9: mouse thymus tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody (Catalog # A00999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.
https://www.bosterbio.com/products/primary-antibodies/anti-glutathione-reductase-picoband-trade-antibody-a01479-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-wb-testing-1.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® Western blot analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human MOLT-4 whole cell lysates,<br>
Lane 4: human RT4 whole cell lysates,<br>
Lane 5: human Daudi whole cell lysates,<br>
Lane 6: human K562 whole cell lysates,<br>
Lane 7: rat kidney tissue lysates,<br>
Lane 8: mouse kidney tissue lysates,<br>
Lane 9: mouse colon tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Reductase antigen affinity purified polyclonal antibody (Catalog # A01479-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Reductase at approximately 52 kDa. The expected band size for Glutathione Reductase is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-ihc-testing-2.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® IHC analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Glutathione Reductase was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Reductase Antibody (A01479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-ihc-testing-3.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® IHC analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Glutathione Reductase was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Reductase Antibody (A01479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-ihc-testing-4.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® IHC analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Glutathione Reductase was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Reductase Antibody (A01479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-ihc-testing-5.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® IHC analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Glutathione Reductase was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Reductase Antibody (A01479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-if-testing-6.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® IF analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). <br>
Glutathione Reductase was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Glutathione Reductase Antibody (A01479-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01479-1-gsr-primary-antibodies-fcm-testing-7_1.jpgAnti-Glutathione Reductase/GSR Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Glutathione Reductase antibody (A01479-1). <br>
Overlay histogram showing HL-60 cells stained with A01479-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutathione Reductase Antibody (A01479-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-tim-3-picoband-trade-antibody-a00657-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00657-1-TIM_3-primary-antibodies-WB-testing-1.jpgAnti-TIM 3/HAVCR2 Antibody Picoband® Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Raji whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ide-picoband-trade-antibody-a01358-2-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01358-2-ide-primary-antibodies-wb-testing-1.jpgAnti-Insulin degrading enzyme/IDE Antibody Picoband® Western blot analysis of IDE using anti-IDE antibody (A01358-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Caco-2 whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified polyclonal antibody (Catalog # A01358-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01358-2-IDE-primary-antibodies-IHC-testing-2.jpgAnti-Insulin degrading enzyme/IDE Antibody Picoband® IHC analysis of IDE using anti-IDE antibody (A01358-2).
<br>IDE was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDE Antibody (A01358-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01358-2-IDE-primary-antibodies-IHC-testing-3.jpgAnti-Insulin degrading enzyme/IDE Antibody Picoband® IHC analysis of IDE using anti-IDE antibody (A01358-2).
<br>IDE was detected in paraffin-embedded section of rat pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDE Antibody (A01358-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01358-2-ide-primary-antibodies-fc-testing-4.jpgAnti-Insulin degrading enzyme/IDE Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-IDE antibody (A01358-2).<br>Overlay histogram showing Hela cells stained with A01358-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDE Antibody (A01358-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01358-2-ide-primary-antibodies-fc-testing-5.jpgAnti-Insulin degrading enzyme/IDE Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-IDE antibody (A01358-2).<br>Overlay histogram showing CACO-2 cells stained with A01358-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDE Antibody (A01358-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-igf2r-picoband-trade-antibody-a00951-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-wb-testing-1.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® Western blot analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified polyclonal antibody (Catalog # A00951) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 274 kDa. The expected band size for IGF2R is at 274 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-ihc-testing-2.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® IHC analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
IGF2R was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-ihc-testing-3.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® IHC analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
IGF2R was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-ihc-testing-4.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® IHC analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
IGF2R was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-ihc-testing-5.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® IHC analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
IGF2R was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-if-testing-6.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® IF analysis of IGF2R using anti-IGF2R antibody (A00951). <br>
IGF2R was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-igf2r-primary-antibodies-fcm-testing-7_1.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-IGF2R antibody (A00951).<br>
Overlay histogram showing HepG2 cells stained with A00951 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF2R Antibody (A00951,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-il32-picoband-trade-antibody-a03286-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03286-IL32-primary-antibodies-WB-testing-1.jpgAnti-IL32 Antibody Picoband® Western blot analysis of IL32 using anti-IL32 antibody (A03286).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
<br>Lane 1: recombinant human IL32 protein 1ng.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL32 at approximately 19KD. The expected band size for IL32 is at 15KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03286-il32-primary-antibodies-elisa-testing-2.jpgAnti-IL32 Antibody Picoband® Sandwich ELISA - Recombinant human IL32 protein standard curve.<br>
Use in combination with reagents from Human IL32 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1599).
https://www.bosterbio.com/products/primary-antibodies/anti-cd11b-picoband-trade-antibody-a00144-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00144-1-cd11b-primary-antibodies-wb-testing-1_1.jpgAnti-CD11b/ITGAM Antibody Picoband® Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse spleen tissue lysates, <br>
Lane 2: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (A00144-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD11b at approximately 179 kDa. The expected band size for CD11b is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00144-1-cd11b-primary-antibodies-ihc-testing-2.jpgAnti-CD11b/ITGAM Antibody Picoband® IHC analysis of CD11b using anti-CD11b antibody (A00144-1). <br>
CD11b was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD11b Antibody (A00144-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00144-1-cd11b-primary-antibodies-fcm-testing-3.jpgAnti-CD11b/ITGAM Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CD11b antibody (A00144-1). <br>
Overlay histogram showing U937 cells stained with A00144-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (A00144-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kiss1-receptor-antibody-a01364-1-boster.html2026-03-24T05:16:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01364-1-KiSS1_receptor-primary-antibodies-WB-testing-1.jpgAnti-KiSS1 receptor/KISS1R Antibody Picoband® Western blot analysis of KiSS1 receptor using anti-KiSS1 receptor antibody (A01364-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human WISH whole cell lysates, <br>Lane 2: human HepG2 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KiSS1 receptor antigen affinity purified polyclonal antibody (Catalog # A01364-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KiSS1 receptor at approximately 43KD. The expected band size for KiSS1 receptor is at 43KD.
https://www.bosterbio.com/products/primary-antibodies/anti-lactoferrin-picoband-trade-antibody-a00633-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-ltf-primary-antibodies-wb-testing-1_1.jpgAnti-Lactoferrin/LTF Antibody Picoband®Western blot analysis of LTF using anti-LTF antibody (A00633-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTF antigen affinity purified polyclonal antibody (A00633-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LTF at approximately 85 kDa. The expected band size for LTF is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-ltf-primary-antibodies-ihc-testing-1.jpgAnti-Lactoferrin/LTF Antibody Picoband®IHC analysis of LTF using anti-LTF antibody (A00633-1). <br>
LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LTF Antibody (A00633-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-fnagi-16-1309115-g002.jpgAnti-Lactoferrin/LTF Antibody Picoband®LTF, a ferroptosis-related gene, is identified in aging cochleae. (A) The volcano diagram of shows 43385 DEGs between the old group ( ~864316) and young group ( ~864308). (B) The heatmap shows 28 statistically significant DEGs. (C) Functional analysis of the statistically significant DEGs. (D) LTF, the hub gene, is obtained from the intersection of Up-Gens, Down-Gens (based on the statistically significant DEGs), and FRGs. (E) The PPI network shows that 12 proteins are interacting with LTF.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10809180/&orig_host=www.ncbi.nlm.nih.gov'>38282692</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-fnagi-16-1309115-g003.jpgAnti-Lactoferrin/LTF Antibody Picoband®TF-miRNA-mRNA network in cochlear ferroptosis. (A) The volcano diagram of shows 7512 DEMs between the old group ( ~1095954) and young group ( ~1095948). (B) The heatmap shows 12 statistically significant DEMs. (C–F) TFs predicted by mmu-mir-130b (C) , mmu-mir-205 (D) , hsa-mir-130b (E) , and hsa-mir-205 (F) . (G) CEBPA and CEBPB were predicted by mir-130b. (H) STAT3 was predicted by mir-205. (I,J) The top 20 TFs predicted by LTF in mice (I) and humans (J) , CEBPA (Red box) was identified through the intersection of (I,J) , and TFs predicted by miRNAs in (G,H) . (K) The regulatory network was constructed.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10809180/&orig_host=www.ncbi.nlm.nih.gov'>38282692</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-fnagi-16-1309115-g004.jpgAnti-Lactoferrin/LTF Antibody Picoband®Ferroptosis in aging HEI-OC1 cells. (A) SA-β-gal staining in HEI-OC1 cells treated with different concentrations of D-gal for 48 h. (B) Quantification of SA-β-positive cells in (A) . Compared with the control group, the percentage of positive cells is observed to have a statistical difference at 20 mg/mL D-gal ( p < 0.001; N = 3). (C–E) Compared with the control group, the expression of Fe 2+ (C) and MDA (D) is increased in aging HEI-OC1 cells with 20 mg/mL D-gal ( p = 0.006 and p < 0.001; N = 3). Oppositely, the expression of LTF (E) is decreased ( p < 0.001; N = 3). More importantly, Liproxstatin-1 reversed the above phenomenon of ferroptosis in aging HEI-OC1 cells with 20 mg/mL D-gal ( p = 0.038, p = 0.012, and p = 0.046; N = 3). (F) Immunofluorescence staining also shows decreased expression of LTF in aging HEI-OC1 cells with 20 mg/mL D-gal (LTF-green, DAPI-blue). Ctrl, control; Lip, Liproxstatin-1.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10809180/&orig_host=www.ncbi.nlm.nih.gov'>38282692</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-fnagi-16-1309115-g005.jpgAnti-Lactoferrin/LTF Antibody Picoband®Ferroptosis in aging cochlear explants. (A) SA-β-gal staining in the basement membrane treated with different concentrations of D-gal for 48 h. (B) Quantification of SA-β-positive cells in (A) . Compared with the control group, the percentage of positive cells is observed to have a statistical difference at 30 mg/mL D-gal ( p < 0.001; N = 3). (C–E) Compared with the control group, the expression of Fe 2+ (C) and MDA (D) is increased in aging cochlear explants with 30 mg/mL D-gal ( p < 0.001; N = 5). Oppositely, the expression of LTF (E) is decreased in aging cochlear explants with 30 mg/mL D-gal ( p < 0.001; N = 5). OHC, outer hair cell; IHC, inner hair cell; Ctrl, control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10809180/&orig_host=www.ncbi.nlm.nih.gov'>38282692</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-fnagi-16-1309115-g006.jpgAnti-Lactoferrin/LTF Antibody Picoband®Ferroptosis in aging cochleae. (A) Compared with young mice (2mo), the distinctly increased ABR thresholds are shown in old mice (10mo) at all frequencies ( p < 0.001; N = 6 mice). (B–D) Compared with young mice, the expression of Fe 2+ (B) and MDA (C) is increased in aging cochleae ( p < 0.001; N = 5). Oppositely, the expression of LTF (D) is decreased ( p < 0.005; N = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10809180/&orig_host=www.ncbi.nlm.nih.gov'>38282692</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-ltf-primary-antibodies-if-testing-1.jpgAnti-Lactoferrin/LTF Antibody Picoband®IF analysis of LTF using anti-LTF antibody (A00633-1). <br>
LTF was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LTF Antibody (A00633-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00633-1-lactoferrinltf-primary-antibodies-wb-testing-6.pngAnti-Lactoferrin/LTF Antibody Picoband® Western blot analysis of Lactoferrin/LTF using anti-Lactoferrin/LTF Antibody (A00633-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Total LTF from mouse neutrophil cell lysates. <br>Lane 2: degranulated LTF. Ratio 1:20.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lactoferrin/LTF antigen affinity purified polyclonal antibody (A00633-1) at 1:2000 1h overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:3000 dilution at RT for 1 hour. The signal is developed using HRP. A specific band was detected for Lactoferrin/LTF at approximately 77 kDa. The expected band size for Lactoferrin/LTF is at 77 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-map1lc3a-picoband-trade-antibody-a01543-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-WB-testing-1.jpgAnti-MAP1LC3A Antibody Picoband® Western blot analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A antigen affinity purified polyclonal antibody (Catalog # A01543-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18KD. The expected band size for MAP1LC3A is at 14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01543-1-map1lc3a-primary-antibodies-ihc-testing-7.jpgAnti-MAP1LC3A Antibody Picoband®IHC analysis of LC3A/MAP1LC3A using anti-LC3A/MAP1LC3A antibody (A01543-1). <br>LC3A/MAP1LC3A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LC3A/MAP1LC3A Antibody (A01543-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-IHC-testing-2.jpgAnti-MAP1LC3A Antibody Picoband® IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>MAP1LC3A was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-IHC-testing-3.jpgAnti-MAP1LC3A Antibody Picoband® IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>MAP1LC3A was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-IHC-testing-4.jpgAnti-MAP1LC3A Antibody Picoband® IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>MAP1LC3A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-IHC-testing-5.jpgAnti-MAP1LC3A Antibody Picoband® IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>MAP1LC3A was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01543-1-MAP1LC3A-primary-antibodies-IHC-testing-6.jpgAnti-MAP1LC3A Antibody Picoband® IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-1).
<br>MAP1LC3A was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP1LC3A Antibody (A01543-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-myelin-protein-zero-picoband-trade-antibody-a00997-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00997-1-Myelin_Protein_Zero-primary-antibodies-WB-testing-1.jpgAnti-Myelin Protein Zero/MPZ Antibody Picoband® Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human U-87MG whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin Protein Zero antigen affinity purified polyclonal antibody (Catalog # A00997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin Protein Zero at approximately 28KD. The expected band size for Myelin Protein Zero is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mvp-picoband-trade-antibody-a00642-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00642-1-mvp-primary-antibodies-wb-testing-1.jpgAnti-MVP Antibody Picoband® Western blot analysis of MVP using anti-MVP antibody (A00642-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human HepG2 whole cell lysates, <br>Lane 3: human A549 whole cell lysates, <br>Lane 4: human PANC-1 whole cell lysates, <br>Lane 5: human SGC-7901 whole cell lysates, <br>Lane 6: human MDA-MB-231 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody (Catalog # A00642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00642-1-mvp-primary-antibodies-wb-testing-2.jpgAnti-MVP Antibody Picoband® Western blot analysis of MVP using anti-MVP antibody (A00642-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: rat kidney tissue lysates,<br>Lane 4: mouse spleen tissue lysates,<br>Lane 5: mouse lung tissue lysates,<br>Lane 6: mouse kidney tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody (Catalog # A00642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00642-1-MVP-primary-antibodies-IHC-testing-3.jpgAnti-MVP Antibody Picoband® IHC analysis of MVP using anti-MVP antibody (A00642-1).
<br>MVP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MVP Antibody (A00642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00642-1-MVP-primary-antibodies-IHC-testing-4.jpgAnti-MVP Antibody Picoband® IHC analysis of MVP using anti-MVP antibody (A00642-1).
<br>MVP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MVP Antibody (A00642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00642-1-MVP-primary-antibodies-IHC-testing-5.jpgAnti-MVP Antibody Picoband® IHC analysis of MVP using anti-MVP antibody (A00642-1).
<br>MVP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MVP Antibody (A00642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00642-1-MVP-primary-antibodies-IHC-testing-6.jpgAnti-MVP Antibody Picoband® IHC analysis of MVP using anti-MVP antibody (A00642-1).
<br>MVP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MVP Antibody (A00642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00642-1-mvp-primary-antibodies-fc-testing-7.jpgAnti-MVP Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-MVP antibody (A00642-1).
<br>Overlay histogram showing Hela cells stained with A00642-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (A00642-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00642-1-mvp-primary-antibodies-fc-testing-8.jpgAnti-MVP Antibody Picoband® Flow Cytometry analysis of U-87 cells using anti-MVP antibody (A00642-1).
<br>Overlay histogram showing U-87 cells stained with A00642-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (A00642-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00642-1-mvp-primary-antibodies-if-testing-9.jpgAnti-MVP Antibody Picoband® IF analysis of MVP using anti-MVP antibody (A00642-1). <br> MVP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MVP Antibody (A00642-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-mylk-picoband-trade-antibody-a01697-2-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01697-2-mylk-primary-antibodies-wb-testing-1.jpgAnti-Myosin light chain kinase/MYLK Antibody Picoband®Western blot analysis of MYLK using anti-MYLK antibody (A01697-2). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human SiHa whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK antigen affinity purified polyclonal antibody (A01697-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYLK at approximately 135 kDa. The expected band size for MYLK is at 211 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01697-2-mylk-primary-antibodies-ihc-testing-1.jpgAnti-Myosin light chain kinase/MYLK Antibody Picoband®IHC analysis of MYLK using anti-MYLK antibody (A01697-2). <br>MYLK was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYLK Antibody (A01697-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01697-2-mylk-primary-antibodies-if-testing-1.jpgAnti-Myosin light chain kinase/MYLK Antibody Picoband®IF analysis of MYLK using anti-MYLK antibody (A01697-2). <br>
MYLK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYLK Antibody (A01697-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ncr1-picoband-trade-antibody-a01516-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01516-1-NCR1-primary-antibodies-WB-testing-1.jpgAnti-NCR1 Antibody Picoband® Western blot analysis of NCR1 using anti-NCR1 antibody (A01516-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat spleen tissue lysates,<br>Lane 2: mouse spleen tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR1 antigen affinity purified polyclonal antibody (Catalog # A01516-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCR1 at approximately 36KD. The expected band size for NCR1 is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01516-1-NCR1-primary-antibodies-IHC-testing-2.jpgAnti-NCR1 Antibody Picoband® IHC analysis of NCR1 using anti-NCR1 antibody (A01516-1).
<br>NCR1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody (A01516-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01516-1-NCR1-primary-antibodies-IHC-testing-3.jpgAnti-NCR1 Antibody Picoband® IHC analysis of NCR1 using anti-NCR1 antibody (A01516-1).
<br>NCR1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody (A01516-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-1-ncr1-primary-antibodies-ihc-testing-4.jpgAnti-NCR1 Antibody Picoband® IHC analysis of NCR1 using anti-NCR1 antibody (A01516-1). <br> NCR1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody (A01516-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-1-ncr1-primary-antibodies-ihc-testing-5.jpgAnti-NCR1 Antibody Picoband® IHC analysis of NCR1 using anti-NCR1 antibody (A01516-1). <br>
NCR1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody (A01516-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-1-ncr1-primary-antibodies-fc-testing-1.jpgAnti-NCR1 Antibody Picoband®Flow Cytometry analysis of mouse spleen cells using anti-NCR1 antibody (A01516-1).<br>
Overlay histogram showing mouse spleen cells stained with A01516-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NCR1 Antibody (A01516-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nfib-nf1b2-picoband-trade-antibody-a01537-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01537-1-NFIB-primary-antibodies-WB-testing-1.jpgAnti-NFIB/NF1B2 Antibody Picoband® Western blot analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody (A01537-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: rat PC-12 whole cell lysates, <br>Lane 3: mouse lung tissue lysates,<br>Lane 4: mouse ovary tissue lysates,<br>Lane 5: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIB/NF1B2 antigen affinity purified polyclonal antibody (Catalog # A01537-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIB/NF1B2 at approximately 50KD. The expected band size for NFIB/NF1B2 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01537-1-NFIB-primary-antibodies-IHC-testing-4.jpgAnti-NFIB/NF1B2 Antibody Picoband® IHC analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody (A01537-1).
<br>NFIB/NF1B2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIB/NF1B2 Antibody (A01537-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01537-1-NFIB-primary-antibodies-IHC-testing-5.jpgAnti-NFIB/NF1B2 Antibody Picoband® IHC analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody (A01537-1).
<br>NFIB/NF1B2 was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIB/NF1B2 Antibody (A01537-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01537-1-NFIB-primary-antibodies-IHC-testing-6.jpgAnti-NFIB/NF1B2 Antibody Picoband® IHC analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody (A01537-1).
<br>NFIB/NF1B2 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIB/NF1B2 Antibody (A01537-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-neurexin-1-antibody-a01490-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01490-1-nrxn1-primary-antibodies-wb-testing-1.jpgAnti-Neurexin 1/NRXN1 Antibody Picoband® Western blot analysis of Neurexin 1/NRXN1 using anti-Neurexin 1/NRXN1 antibody (A01490-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: human U251 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1/NRXN1 antigen affinity purified polyclonal antibody (Catalog # A01490-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurexin 1/NRXN1 at approximately 160-170 kDa. The expected band size for Neurexin 1/NRXN1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01490-1-nrxn1-primary-antibodies-fcm-testing-2.jpgAnti-Neurexin 1/NRXN1 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-Neurexin 1/NRXN1 antibody (A01490-1). <br>Overlay histogram showing SH-SY5Y cells stained with A01490-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Neurexin 1/NRXN1 Antibody (A01490-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-parn-picoband-trade-antibody-a01501-2-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01501-2-PARN-primary-antibodies-WB-testing-1.jpgAnti-PARN Antibody Picoband® Western blot analysis of PARN using anti-PARN antibody (A01501-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human COLO-320 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates, <br>Lane 5: human PANC-1 whole cell lysates, <br>Lane 6: human SGC-7901 whole cell lysates, <br>Lane 7: human MDA-MB-231 whole cell lysates, <br>Lane 8: rat kidney tissue lysates,<br>Lane 9: mouse heart tissue lysates,<br>Lane 10: mouse kidney tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARN antigen affinity purified polyclonal antibody (Catalog # A01501-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARN at approximately 78KD. The expected band size for PARN is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-2.jpgAnti-PARN Antibody Picoband® IHC analysis of PARN using anti-PARN antibody (A01501-2).
<br>PARN was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-3.jpgAnti-PARN Antibody Picoband® IHC analysis of PARN using anti-PARN antibody (A01501-2).
<br>PARN was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01501-2-PARN-primary-antibodies-IHC-testing-4.jpgAnti-PARN Antibody Picoband® IHC analysis of PARN using anti-PARN antibody (A01501-2).
<br>PARN was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01501-2-parn-primary-antibodies-if-testing-5.pngAnti-PARN Antibody Picoband® IF analysis of PARN using anti-PARN antibody (A01501-2).<br> PARN was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PARN Antibody (A01501-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01501-2-parn-primary-antibodies-fc-testing-6.pngAnti-PARN Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PARN antibody (A01501-2). <br> Overlay histogram showing A431 cells stained with A01501-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARN Antibody (A01501-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pde4d-picoband-trade-antibody-a01111-1-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01111-1-PDE4D-primary-antibodies-WB-testing-1.jpgAnti-PDE4D Antibody Picoband® Western blot analysis of PDE4D using anti-PDE4D antibody (A01111-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4D antigen affinity purified polyclonal antibody (Catalog # A01111-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE4D at approximately 91KD. The expected band size for PDE4D is at 91KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01111-1-PDE4D-primary-antibodies-IHC-testing-2.jpgAnti-PDE4D Antibody Picoband® IHC analysis of PDE4D using anti-PDE4D antibody (A01111-1).
<br>PDE4D was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDE4D Antibody (A01111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01111-1-PDE4D-primary-antibodies-IHC-testing-3.jpgAnti-PDE4D Antibody Picoband® IHC analysis of PDE4D using anti-PDE4D antibody (A01111-1).
<br>PDE4D was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDE4D Antibody (A01111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01111-1-PDE4D-primary-antibodies-IHC-testing-4.jpgAnti-PDE4D Antibody Picoband® IHC analysis of PDE4D using anti-PDE4D antibody (A01111-1).
<br>PDE4D was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDE4D Antibody (A01111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01111-1-pde4d-primary-antibodies-fc-testing-5.jpgAnti-PDE4D Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-PDE4D antibody (A01111-1).<br>Overlay histogram showing A549 cells stained with A01111-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (A01111-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01111-1-pde4d-primary-antibodies-fc-testing-6.jpgAnti-PDE4D Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PDE4D antibody (A01111-1).<br>Overlay histogram showing Hela cells stained with A01111-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (A01111-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pdk1-picoband-trade-antibody-a01268-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01268-1-pdk1-primary-antibodies-wb-testing-1.jpgAnti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband® Western blot analysis of PDK1 using anti-PDK1 antibody (A01268-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody (Catalog # A01268-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58 kDa. The expected band size for PDK1 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01268-1-pdk1-primary-antibodies-ihc-testing-3.jpgAnti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®IHC analysis of PDK1 using anti-PDK1 antibody (A01268-1). <br>PDK1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDK1 Antibody (A01268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01268-1-PDK1-primary-antibodies-IHC-testing-2.jpgAnti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband® IHC analysis of PDK1 using anti-PDK1 antibody (A01268-1).
<br>PDK1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDK1 Antibody (A01268-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-pbp-picoband-trade-antibody-a01668-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01668-pbp-primary-antibodies-wb-testing-1.jpgAnti-PBP/PEBP1 Antibody Picoband® Western blot analysis of PBP using anti-PBP antibody (A01668). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human 293T whole cell lysates, <br>
Lane 5: mouse testis lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBP antigen affinity purified polyclonal antibody (Catalog # A01668) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBP at approximately 21 kDa. The expected band size for PBP is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-2.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-3.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-4.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).<br>PBP was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-5.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-6.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-7.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).<br>PBP was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-8.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-9.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).
<br>PBP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01668-PBP-primary-antibodies-IHC-testing-1.jpgAnti-PBP/PEBP1 Antibody Picoband® IHC analysis of PBP using anti-PBP antibody (A01668).<br>PBP was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PBP Antibody (A01668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01668-pbp-primary-antibodies-fc-testing-11.ipg.pngAnti-PBP/PEBP1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PBP antibody (A01668). <br> Overlay histogram showing HepG2 cells stained with A01668 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PBP Antibody (A01668,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pla2g6-antibody-a01648-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01648-1-pla2g6-primary-antibodies-wb-testing-1_1.jpgAnti-PLA2G6 Antibody Picoband®Western blot analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G6 antigen affinity purified polyclonal antibody (A01648-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLA2G6 at approximately 84 kDa. The expected band size for PLA2G6 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01648-1-pla2g6-primary-antibodies-if-testing-1.jpgAnti-PLA2G6 Antibody Picoband®IF analysis of PLA2G6 using anti-PLA2G6 antibody (A01648-1). <br>
PLA2G6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLA2G6 Antibody (A01648-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-plac9-picoband-trade-antibody-a17853-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17853-1-PLAC9-primary-antibodies-WB-testing-1.jpgAnti-PLAC9 Antibody Picoband® Western blot analysis of PLAC9 using anti-PLAC9 antibody (A17853-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysates,<br>Lane 2: human placenta tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAC9 antigen affinity purified polyclonal antibody (Catalog # A17853-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAC9 at approximately 17KD. The expected band size for PLAC9 is at 10KD.
https://www.bosterbio.com/products/primary-antibodies/anti-upa-receptor-picoband-trade-antibody-a00993-2-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-2-plaur-primary-antibodies-wb-testing-1.jpgAnti-uPA Receptor/Plaur Antibody Picoband®Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: rat heart tissue lysates,<br>
Lane 3: rat C6 whole cell lysates,<br>
Lane 4: mouse brain tissue lysates,<br>
Lane 5: mouse thymus tissue lysates,<br>
Lane 6: mouse heart tissue lysates,<br>
Lane 7: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (A00993-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 50 kDa. The expected band size for uPA Receptor is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-2-plaur-primary-antibodies-ihc-testing-1.jpgAnti-uPA Receptor/Plaur Antibody Picoband®IHC analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-2). <br>uPA Receptor was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-uPA Receptor Antibody (A00993-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-2-plaur-primary-antibodies-ihc-testing-2.jpgAnti-uPA Receptor/Plaur Antibody Picoband®IHC analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-2). <br>uPA Receptor was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-uPA Receptor Antibody (A00993-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-2-plaur-primary-antibodies-fcm-testing-1.jpgAnti-uPA Receptor/Plaur Antibody Picoband®Flow Cytometry analysis of PC-12 cells using anti-uPA Receptor antibody (A00993-2). <br>
Overlay histogram showing PC-12 cells stained with A00993-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor Antibody (A00993-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pms2-antibody-a01028-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01028-PMS2-primary-antibodies-WB-testing-1.jpgAnti-PMS2 Antibody Picoband® Western blot analysis of PMS2 using anti-PMS2 antibody (A01028).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMS2 antigen affinity purified polyclonal antibody (Catalog # A01028) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMS2 at approximately 115KD. The expected band size for PMS2 is at 96KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dna-polymerase-iota-picoband-trade-antibody-a01376-1-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01376-1-DNA_Polymerase_iota-primary-antibodies-WB-testing-1.jpgAnti-DNA Polymerase iota/POLI Antibody Picoband® Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human A549 whole cell lysates, <br>Lane 4: human SK-OV-3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody (Catalog # A01376-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01376-1-DNA_Polymerase_iota-primary-antibodies-WB-testing-2.jpgAnti-DNA Polymerase iota/POLI Antibody Picoband® Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody (A01376-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysates,<br>Lane 2: rat testis tissue lysates,<br>Lane 3: rat kidney tissue lysates,<br>Lane 4: rat stomach tissue lysates,<br>Lane 5: mouse testis tissue lysates,<br>Lane 6: mouse testis tissue lysates,<br>Lane 7: mouse kidney tissue lysates,<br>Lane 8: mouse stomach tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody (Catalog # A01376-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dna-polymerase-lambda-picoband-trade-antibody-a00959-2-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00959-2-DNA_Polymerase_lambda-primary-antibodies-WB-testing-1.jpgAnti-DNA Polymerase lambda/POLL Antibody Picoband® Western blot analysis of DNA Polymerase lambda using anti-DNA Polymerase lambda antibody (A00959-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human COLO-320 whole cell lysates, <br>Lane 3: human MCF-7 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase lambda antigen affinity purified polyclonal antibody (Catalog # A00959-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase lambda at approximately 70KD. The expected band size for DNA Polymerase lambda is at 63KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00959-2-dna_polymerase_lambda-primary-antibodies-fc-testing-2.jpg.pngAnti-DNA Polymerase lambda/POLL Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-DNA Polymerase lambda antibody (A00959-2). <br> Overlay histogram showing U20S cells stained with A00959-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNA Polymerase lambda Antibody (A00959-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-polr2a-antibody-a01029-boster.html2026-03-24T05:16:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01029-POLR2A-primary-antibodies-IHC-testing-1.jpgAnti-RNA polymerase II RPB1/POLR2A Antibody IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
<br>POLR2A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01029-POLR2A-primary-antibodies-IHC-testing-2.jpgAnti-RNA polymerase II RPB1/POLR2A Antibody IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
<br>POLR2A was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01029-POLR2A-primary-antibodies-IHC-testing-3.jpgAnti-RNA polymerase II RPB1/POLR2A Antibody IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
<br>POLR2A was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01029-POLR2A-primary-antibodies-IHC-testing-4.jpgAnti-RNA polymerase II RPB1/POLR2A Antibody IHC analysis of POLR2A using anti-POLR2A antibody (A01029).
<br>POLR2A was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-ampk-alpha-1-antibody-a00994-3-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00994-3-prkaa1-primary-antibodies-wb-testing-1.jpgAnti-AMPK alpha 1/PRKAA1 Antibody Picoband® Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SiHa whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human RT4 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat PC-12 whole cell lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 64 kDa. The expected band size for AMPK alpha 1 is at 64 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-protein-s-picoband-trade-antibody-a01568-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01568-1-Protein_S-primary-antibodies-WB-testing-1.jpgAnti-Protein S/PROS1 Antibody Picoband® Western blot analysis of Protein S using anti-Protein S antibody (A01568-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein S antigen affinity purified polyclonal antibody (Catalog # A01568-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein S at approximately 75KD. The expected band size for Protein S is at 75KD.
https://www.bosterbio.com/products/primary-antibodies/anti-prx-picoband-trade-antibody-a01686-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01686-prx-primary-antibodies-wb-testing-1.jpgAnti-PRX Antibody Picoband® Western blot analysis of PRX using anti-PRX antibody (A01686). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: humna U2OS whole cell lysates,<br>
Lane 4: human U251 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRX antigen affinity purified polyclonal antibody (Catalog # A01686) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRX at approximately 155 kDa. The expected band size for PRX is at 148 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01686-prx-primary-antibodies-fcm-testing-2.jpgAnti-PRX Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-PRX antibody (A01686). <br>
Overlay histogram showing K562 cells stained with A01686 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRX Antibody (A01686, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01686-12951_2022_1337_fig12_html.pngAnti-PRX Antibody Picoband®SPION-mediated magnetic actuation promotes remyelination of regenerated nerve fibers. The expression of myelin-associated structural proteins (periaxin and MBP) was detected by immunohistochemistry in different experimental groups at 14 ( a ) and 21 ( b ) days after sciatic nerve crush injury. c The protein expression levels in immunohistochemical images were quantitatively analyzed to evaluate the effect of different treatments on nerve remyelination. d The myelin thickness of regenerated myelinated nerve fibers was measured 3 weeks after nerve crush injury to evaluate the effect of different treatment factors on nerve remyelination from a morphological perspective. e , f The relative protein expression of periaxin and MBP in different experimental groups was measured by WB at the above time points to validate the immunohistochemical results. Each experiment was carried out in triplicate. The values are represented as the mean ± SD. Scale bar = 50 µm in panels a and b. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-022-01337-5'>35351151</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-pleiotrophin-picoband-trade-antibody-a01368-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01368-1-Pleiotrophin-primary-antibodies-WB-testing-1.jpgAnti-Pleiotrophin/PTN Antibody Picoband® Western blot analysis of Pleiotrophin using anti-Pleiotrophin antibody (A01368-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleiotrophin antigen affinity purified polyclonal antibody (Catalog # A01368-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleiotrophin at approximately 19KD. The expected band size for Pleiotrophin is at 19KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ralbp1-picoband-trade-antibody-a01403-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01403-1-RALBP1-primary-antibodies-WB-testing-1.jpgAnti-RALBP1 Antibody Picoband® Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human HepG2 whole cell lysates, <br>Lane 4: human SGC-7901 whole cell lysates, <br>Lane 5: human 22RV1 whole cell lysates, <br>Lane 6: rat lung tissue lysates,<br>Lane 7: mouse lung tissue lysates,<br>Lane 8: mouse testis tissue lysates,<br>Lane 9: mouse ovary tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-ihc-testing-2.jpgAnti-RALBP1 Antibody Picoband® IHC analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). <br> RALBP1 was detected in paraffin-embedded section of human ovary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RALBP1 Antibody (A01403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-ihc-testing-3.jpgAnti-RALBP1 Antibody Picoband® IHC analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). <br> RALBP1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RALBP1 Antibody (A01403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-ihc-testing-4.jpgAnti-RALBP1 Antibody Picoband® IHC analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). <br> RALBP1 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RALBP1 Antibody (A01403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-ihc-testing-5.jpgAnti-RALBP1 Antibody Picoband® IHC analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). <br> RALBP1 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RALBP1 Antibody (A01403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-fc-testing-6.pngAnti-RALBP1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RALBP1 antibody (A01403-1). <br> Overlay histogram showing U20S cells stained with A01403-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RALBP1 Antibody (A01403-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01403-1-ralbp1-primary-antibodies-if-testing-7.jpgAnti-RALBP1 Antibody Picoband® IF analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). <br>
RALBP1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RALBP1 Antibody (A01403-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-regucalcin-picoband-trade-antibody-a05900-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05900-1-Regucalcin-primary-antibodies-WB-testing-1.jpgAnti-Regucalcin/RGN Antibody Picoband® Western blot analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat liver tissue lysates,<br>Lane 2: mouse liver tissue lysates,<br>Lane 3: human SMMC-7721 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Regucalcin antigen affinity purified polyclonal antibody (Catalog # A05900-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Regucalcin at approximately 33, 43KD. The expected band size for Regucalcin is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05900-1-Regucalcin-primary-antibodies-IHC-testing-2.jpgAnti-Regucalcin/RGN Antibody Picoband® IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
<br>Regucalcin was detected in paraffin-embedded section of mouse liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05900-1-Regucalcin-primary-antibodies-IHC-testing-3.jpgAnti-Regucalcin/RGN Antibody Picoband® IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
<br>Regucalcin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05900-1-Regucalcin-primary-antibodies-IHC-testing-4.jpgAnti-Regucalcin/RGN Antibody Picoband® IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
<br>Regucalcin was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-rpgr-picoband-trade-antibody-a01522-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01522-RPGR-primary-antibodies-WB-testing-1.jpgAnti-RPGR Antibody Picoband® Western blot analysis of RPGR using anti-RPGR antibody (A01522).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat liver tissue lysates,<br>Lane 2: rat kidney tissue lysates,<br>Lane 3: rat brain tissue lysates,<br>Lane 4: rat heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGR antigen affinity purified polyclonal antibody (Catalog # A01522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGR at approximately 113KD. The expected band size for RPGR is at 113KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rtel1-antibody-a01544-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01544-1-rtel1-primary-antibodies-wb-testing-1.jpgAnti-RTEL1 Antibody Picoband® Western blot analysis of RTEL1 using anti-RTEL1 antibody (A01544-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysates,<br>Lane 2: rat PC-12 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTEL1 antigen affinity purified polyclonal antibody (Catalog # A01544-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTEL1 at approximately 134KD. The expected band size for RTEL1 is at 134KD.
https://www.bosterbio.com/products/primary-antibodies/anti-runx1t1-eto-antibody-a01792-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01792-1-runx1t1-primary-antibodies-wb-testing-1.jpgAnti-RUNX1T1/ETO Antibody Picoband®Western blot analysis of RUNX1T1 using anti-RUNX1T1 antibody (A01792-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human SH-SY5Y whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1T1 antigen affinity purified polyclonal antibody (A01792-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RUNX1T1 at approximately 70-80 kDa. The expected band size for RUNX1T1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01792-1-RUNX1T1-primary-antibodies-IHC-testing-2.jpgAnti-RUNX1T1/ETO Antibody Picoband® IHC analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1).
<br>RUNX1T1/ETO was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1T1/ETO Antibody (A01792-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01792-1-RUNX1T1-primary-antibodies-IHC-testing-3.jpgAnti-RUNX1T1/ETO Antibody Picoband® IHC analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1).
<br>RUNX1T1/ETO was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1T1/ETO Antibody (A01792-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01792-1-runx1t1-primary-antibodies-if-testing-4.jpg_1.jpgAnti-RUNX1T1/ETO Antibody Picoband® IF analysis of RUNX1T1 using anti-RUNX1T1 antibody (A01792-1). <br> RUNX1T1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RUNX1T1 Antibody (A01792-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01792-1-runx1t1-primary-antibodies-ip-testing-1.jpgAnti-RUNX1T1/ETO Antibody Picoband®Immunoprecipitating (IP) RUNX1T1 in SH-SY5Y whole cell lysate.<br>
Western blot analysis of RUNX1T1 using anti-RUNX1T1 antibody (A01792-1); <br>
Lane 1: SH-SY5Y whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-RUNX1T1 antibody in SH-SY5Y whole cell lysate;<br>
Lane 3: anti-RUNX1T1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RUNX1T1 antigen affinity purified polyclonal antibody (A01792-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for RUNX1T1 at approximately 70-80 kDa. The expected band size for RUNX1T1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01792-1-runx1t1-primary-antibodies-fc-testing-5.jpg.pngAnti-RUNX1T1/ETO Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RUNX1T1 antibody (A01792-1). <br> Overlay histogram showing U20S cells stained with A01792-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNX1T1 Antibody (A01792-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-satb1-picoband-trade-antibody-a01312-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01312-1-SATB1-primary-antibodies-WB-testing-1.jpgAnti-SATB1 Antibody Picoband® Western blot analysis of SATB1 using anti-SATB1 antibody (A01312-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat thymus tissue lysates,<br>Lane 2: mouse thymus tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB1 antigen affinity purified polyclonal antibody (Catalog # A01312-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB1 at approximately 100KD. The expected band size for SATB1 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01312-1-satb1-primary-antibodies-fc-testing-2.jpgAnti-SATB1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SATB1 antibody (A01312-1). <br>Overlay histogram showing PC-3 cells stained with A01312-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SATB1 Antibody (A01312-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-scnn1a-picoband-trade-antibody-a01413-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01413-1-SCNN1A-primary-antibodies-WB-testing-1.jpgAnti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® Western blot analysis of SCNN1A using anti-SCNN1A antibody (A01413-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human COLO-320 whole cell lysates, <br>Lane 2: human HepG2 whole cell lysates, <br>Lane 3: human A549 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCNN1A antigen affinity purified polyclonal antibody (Catalog # A01413-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCNN1A at approximately 76KD. The expected band size for SCNN1A is at 76KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01413-1-scnn1a-primary-antibodies-if-testing-2.jpg.jpgAnti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® IF analysis of SCNN1A using anti-SCNN1A antibody (A01413-1). <br> SCNN1A was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SCNN1A Antibody (A01413-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01413-1-scnn1a-primary-antibodies-if-testing-3.jpg.jpgAnti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® IF analysis of SCNN1A using anti-SCNN1A antibody (A01413-1). <br> SCNN1A was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SCNN1A Antibody (A01413-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-scribble-picoband-trade-antibody-a01651-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01651-SCRIBBLE-primary-antibodies-WB-testing-1.jpgAnti-SCRIBBLE Antibody Picoband® Western blot analysis of SCRIBBLE using anti-SCRIBBLE antibody (A01651).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human MCF-7 whole cell lysates, <br>Lane 2: human COLO-320 whole cell lysates, <br>Lane 3: human 22RV1 whole cell lysates, <br>Lane 4: human SGC-7901 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCRIBBLE antigen affinity purified polyclonal antibody (Catalog # A01651) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCRIBBLE at approximately 240KD. The expected band size for SCRIBBLE is at 175KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01651-scr1b-primary-antibodies-fc-testing-2.jpgAnti-SCRIBBLE Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SCR1B antibody (A01651).<br>Overlay histogram showing A549 cells stained with A01651 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCR1B Antibody (A01651,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01651-scr1b-primary-antibodies-fc-testing-3.jpgAnti-SCRIBBLE Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SCR1B antibody (A01651).<br>Overlay histogram showing HepG2 cells stained with A01651 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCR1B Antibody (A01651,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01651-scr1b-primary-antibodies-if-testing-4.jpgAnti-SCRIBBLE Antibody Picoband® IF analysis of SCRIBBLE using anti-SCRIBBLE antibody (A01651). <br>
SCRIBBLE was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SCRIBBLE Antibody (A01651) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd62p-picoband-trade-antibody-a01241-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01241-1-CD62P-primary-antibodies-WB-testing-1.jpgAnti-CD62P/Selp Antibody Picoband® Western blot analysis of CD62P using anti-CD62P antibody (A01241-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse heart tissue lysates,<br>Lane 2: rat heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (Catalog # A01241-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD62P at approximately 110KD. The expected band size for CD62P is at 91KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01241-1-cd62p-primary-antibodies-fc-testing-2.jpgAnti-CD62P/Selp Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-CD62P antibody (A01241-1). <br>Overlay histogram showing RAW264.7 cells stained with A01241-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD62P Antibody (A01241-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-slc26a4-picoband-trade-antibody-a00919-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00919-1-SLC26A4-primary-antibodies-WB-testing-1.jpgAnti-SLC26A4 Antibody Picoband® Western blot analysis of SLC26A4 using anti-SLC26A4 antibody (A00919-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HK-2 whole cell lysates, <br>Lane 2: human 293T whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A4 antigen affinity purified polyclonal antibody (Catalog # A00919-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC26A4 at approximately 86KD. The expected band size for SLC26A4 is at 86KD.
https://www.bosterbio.com/products/primary-antibodies/anti-slc31a1-ctr1-picoband-trade-antibody-a03447-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03447-1-SLC31A1-primary-antibodies-WB-testing-1.jpgAnti-SLC31A1/CTR1 Antibody Picoband® Western blot analysis of SLC31A1/CTR1 using anti-SLC31A1/CTR1 antibody (A03447-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates, <br>Lane 2: human PANC-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC31A1/CTR1 antigen affinity purified polyclonal antibody (Catalog # A03447-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC31A1/CTR1 at approximately 26KD. The expected band size for SLC31A1/CTR1 is at 21KD.
https://www.bosterbio.com/products/primary-antibodies/anti-brg1-picoband-trade-antibody-a00223-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00223-1-BRG1-primary-antibodies-WB-testing-1.jpgAnti-BRG1/SMARCA4 Antibody Picoband® Western blot analysis of BRG1 using anti-BRG1 antibody (A00223-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human MCF-7 whole cell lysates, <br>Lane 4: human A549 whole cell lysates, <br>Lane 5: human A431 whole cell lysates, <br>Lane 6: human SGC-7901 whole cell lysates, <br>Lane 7: human 22RV1 whole cell lysates, <br>Lane 8: rat small intestine tissue lysates,<br>Lane 9: mouse small intestine tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRG1 antigen affinity purified polyclonal antibody (Catalog # A00223-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRG1 at approximately 220KD. The expected band size for BRG1 is at 185KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00223-1-BRG1-primary-antibodies-IHC-testing-2.jpgAnti-BRG1/SMARCA4 Antibody Picoband® IHC analysis of BRG1 using anti-BRG1 antibody (A00223-1).
<br>BRG1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BRG1 Antibody (A00223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00223-1-BRG1-primary-antibodies-IHC-testing-3.jpgAnti-BRG1/SMARCA4 Antibody Picoband® IHC analysis of BRG1 using anti-BRG1 antibody (A00223-1).
<br>BRG1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BRG1 Antibody (A00223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00223-1-BRG1-primary-antibodies-IHC-testing-4.jpgAnti-BRG1/SMARCA4 Antibody Picoband® IHC analysis of BRG1 using anti-BRG1 antibody (A00223-1).
<br>BRG1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BRG1 Antibody (A00223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00223-1-brg1-primary-antibodies-if-testing-5.jpgAnti-BRG1/SMARCA4 Antibody Picoband® IF analysis of BRG1 using anti-BRG1 antibody (A00223-1) <br> BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-BRG1 Antibody (A00223-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00223-1-brg1-primary-antibodies-if-testing-6.jpg.jpgAnti-BRG1/SMARCA4 Antibody Picoband® IF analysis of BRG1 using anti-BRG1 antibody (A00223-1) <br> BRG1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-BRG1 Antibody (A00223-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00223-1-brg1-primary-antibodies-fc-testing-7.jpg.pngAnti-BRG1/SMARCA4 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-BRG1 antibody (A00223-1). <br> Overlay histogram showing U20S cells stained with A00223-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRG1 Antibody (A00223-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-sphk2-picoband-trade-antibody-a01382-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01382-1-sphk2-primary-antibodies-wb-testing-1_1.jpgAnti-SPHK2 Antibody Picoband®Western blot analysis of SPHK2 using anti-SPHK2 antibody (A01382-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCT116 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: rat kidney tissue lysates, <br>
Lane 5: mouse liver tissue lysates, <br>
Lane 6: mouse kidney tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK2 antigen affinity purified polyclonal antibody (A01382-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPHK2 at approximately 69 kDa. The expected band size for SPHK2 is at 69 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-stam1-antibody-a00864-1-boster.html2026-03-24T05:16:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00864-1-STAM1-primary-antibodies-WB-testing-1.jpgAnti-STAM1 Antibody Picoband® Western blot analysis of STAM1 using anti-STAM1 antibody (A00864-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates, <br>Lane 2: human PANC-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM1 antigen affinity purified polyclonal antibody (Catalog # A00864-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM1 at approximately 59KD. The expected band size for STAM1 is at 59KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00864-1-12885_2023_11648_fig8_html.pngAnti-STAM1 Antibody Picoband®Expression of STAM, ANXA5 and MAD2L2 in HCC cell lines. A The expressions of STAM, ANXA5 and MAD2L2 in normal hepatocytes and hepatoma cell lines were detected using Western blotting. Knocking down STAM, ANXA5 and MAD2L2 inhibited the proliferation and migration of HCC cells. B The knockdown efficiency of STAM, ANXA5 and MAD2L2 was detected using Western Blotting. C Plate cloning experiment showed that the number of cloned cell clusters formed by hepatocellular carcinoma cells after knockdown of STAM, ANXA5 and MAD2L2 was significantly reduced; D The results of CCK8 experiment showed that inhibiting the expression of STAM, ANXA5 and MAD2L2 decreased the proliferative ability of HCC cells; E Scratch assay showed that knockdown of STAM, ANXA5 and MAD2L2 inhibited the migration of hepatocellular carcinoma cells; F Transwell experiment showed that the migration ability of hepatocellular carcinoma cells was weakened after inhibiting the expression of STAM, ANXA5 and MAD2L2 .* P < 0.05, ** P < 0.01, *** P < 0.001, there was no significant difference in ns <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-023-11648-x'>38049741</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-stat2-picoband-trade-antibody-a01360-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01360-stat2-primary-antibodies-wb-testing-1.jpgAnti-STAT2 Antibody Picoband® Western blot analysis of STAT2 using anti-STAT2 antibody (A01360). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human HT1080 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT2 antigen affinity purified polyclonal antibody (Catalog # A01360) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT2 at approximately 113 kDa. The expected band size for STAT2 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01360-stat2-primary-antibodies-if-testing-2.jpgAnti-STAT2 Antibody Picoband® IF analysis of STAT2 using anti-STAT2 antibody (A01360). <br>
STAT2 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT2 Antibody (A01360) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01360-stat2-primary-antibodies-ihc-testing-1.jpgAnti-STAT2 Antibody Picoband®IHC analysis of STAT2 using anti-STAT2 antibody (A01360). <br>
STAT2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT2 Antibody (A01360) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01360-stat2-primary-antibodies-fcm-testing-3.jpgAnti-STAT2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-STAT2 antibody (A01360). <br>
Overlay histogram showing K562 cells stained with A01360 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT2 Antibody (A01360, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-steroid-sulfatase-picoband-trade-antibody-a01198-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01198-1-steroid_sulfatase-primary-antibodies-wb-testing-1.jpgAnti-Steroid sulfatase/STS Antibody Picoband® Western blot analysis of Steroid sulfatase using anti-Steroid sulfatase antibody (A01198-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates,<br>Lane 3: human MCF-7 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates, <br>Lane 5: human PANC-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Steroid sulfatase antigen affinity purified polyclonal antibody (Catalog # A01198-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Steroid sulfatase at approximately 65KD. The expected band size for Steroid sulfatase is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01198-1-sts-primary-antibodies-fcm-testing-2.pngAnti-Steroid sulfatase/STS Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Steroid sulfatase antibody (A01198-1). <br>
Overlay histogram showing Hela cells stained with A01198-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Steroid sulfatase Antibody (A01198-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tlr1-picoband-trade-antibody-a00429-2-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-2-tlr1-primary-antibodies-wb-testing-1.jpgAnti-TLR1 Antibody Picoband® Western blot analysis of TLR1 using anti-TLR1 antibody (A00429-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates,<br>Lane 2: mouse small intestine tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody (Catalog # A00429-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00429-2-TLR1-primary-antibodies-IHC-testing-2.jpgAnti-TLR1 Antibody Picoband® IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2).
<br>TLR1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TLR1 Antibody (A00429-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00429-2-TLR1-primary-antibodies-IHC-testing-3.jpgAnti-TLR1 Antibody Picoband® IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2).
<br>TLR1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TLR1 Antibody (A00429-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00429-2-TLR1-primary-antibodies-IHC-testing-4.jpgAnti-TLR1 Antibody Picoband® IHC analysis of TLR1 using anti-TLR1 antibody (A00429-2).
<br>TLR1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TLR1 Antibody (A00429-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-2-tlr1-primary-antibodies-fc-testing-5.jpgAnti-TLR1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-TLR1 antibody (A00429-2).<br>Overlay histogram showing K562 cells stained with A00429-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (A00429-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-2-tlr1-primary-antibodies-fc-testing-6.jpgAnti-TLR1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody (A00429-2).<br>Overlay histogram showing THP-1 cells stained with A00429-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (A00429-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd134-ox40-antibody-a02495-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02495-cd134-primary-antibodies-wb-testing-1.jpgAnti-CD134/OX40/Tnfrsf4 Antibody Picoband® Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-taci-antibody-a02952-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-1-taci-primary-antibodies-ihc-testing-1.jpgAnti-TACI/TNFRSF13B Antibody IHC analysis of TACI using anti-TACI antibody (A02952-1). <br>
TACI was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-1-taci-primary-antibodies-ihc-testing-2.jpgAnti-TACI/TNFRSF13B Antibody IHC analysis of TACI using anti-TACI antibody (A02952-1). <br>
TACI was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-1-taci-primary-antibodies-ihc-testing-3.jpgAnti-TACI/TNFRSF13B Antibody IHC analysis of TACI using anti-TACI antibody (A02952-1). <br>
TACI was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-1-taci-primary-antibodies-ihc-testing-4.jpgAnti-TACI/TNFRSF13B Antibody IHC analysis of TACI using anti-TACI antibody (A02952-1). <br>
TACI was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-baff-receptor-picoband-trade-antibody-a02865-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02865-1-baff_receptor-primary-antibodies-wb-testing-1.jpgAnti-BAFF Receptor/TNFRSF13C Antibody Picoband® Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Jurkat whole cell lysates, <br>Lane 2: rat spleen tissue lysates,<br>Lane 3: rat thymus tissue lysates,<br>Lane 4: mouse spleen tissue lysates,<br>Lane 5: mouse thymus tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19KD. The expected band size for BAFF Receptor is at 19KD.
https://www.bosterbio.com/products/primary-antibodies/anti-baff-receptor-antibody-a02865-2-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02865-2-BAFF_Receptor-primary-antibodies-WB-testing-1.jpgAnti-BAFF Receptor/Tnfrsf13c Antibody Picoband® Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat thymus tissue lysates,<br>Lane 3: mouse spleen tissue lysates,<br>Lane 4: mouse thymus tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19, 40KD. The expected band size for BAFF Receptor is at 19KD.
https://www.bosterbio.com/products/primary-antibodies/anti-bcma-picoband-trade-antibody-a01014-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01014-1-bcma-primary-antibodies-wb-testing-1_.jpgAnti-BCMA/TNFRSF17 Antibody Picoband® Western blot analysis of BCMA using anti-BCMA antibody (A01014-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Raji whole cell lysates, <br>Lane 2: human Jurkat whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01014-1-BCMA-primary-antibodies-IHC-testing-2.jpgAnti-BCMA/TNFRSF17 Antibody Picoband® IHC analysis of BCMA using anti-BCMA antibody (A01014-1).
<br>BCMA was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BCMA Antibody (A01014-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01014-1-bcma-primary-antibodies-elisa-testing-3_1.jpgAnti-BCMA/TNFRSF17 Antibody Picoband® Sandwich ELISA - Recombinant human BCMA/TNFRSF17 protein standard curve.<br>
Use in combination with reagents from Human BCMA/TNFRSF17 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0661).
https://www.bosterbio.com/products/primary-antibodies/anti-bcma-picoband-trade-antibody-a01014-2-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01014-2-bcma-primary-antibodies-ihc-testing-1.jpgAnti-BCMA/Tnfrsf17 Antibody IHC analysis of BCMA using anti-BCMA antibody (A01014-2). <br>
BCMA was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCMA Antibody (A01014-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01014-2-bcma-primary-antibodies-ihc-testing-2.jpgAnti-BCMA/Tnfrsf17 Antibody IHC analysis of BCMA using anti-BCMA antibody (A01014-2). <br>
BCMA was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCMA Antibody (A01014-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-bcma-picoband-trade-antibody-a01014-3-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01014-3-bcma-primary-antibodies-wb-testing-1_1.jpgAnti-BCMA/Tnfrsf17 Antibody Picoband® Western blot analysis of BCMA using anti-BCMA antibody (A01014-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat thymus tissue lysates,<br>Lane 3: rat lung tissue lysates,<br>Lane 4: rat testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.
https://www.bosterbio.com/products/primary-antibodies/anti-tnfrsf18-picoband-trade-antibody-a03125-2-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03125-2-TNFRSF18-primary-antibodies-WB-testing-1.jpgAnti-TNFRSF18 Antibody Picoband® Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Jurkat whole cell lysates, <br>Lane 2: human K562 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 26KD. The expected band size for TNFRSF18 is at 26KD.
https://www.bosterbio.com/products/primary-antibodies/anti-tpmt-antibody-a00671-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00671-1-TPMT-primary-antibodies-WB-testing-1.jpgAnti-TPMT Antibody Picoband® Western blot analysis of TPMT using anti-TPMT antibody (A00671-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SGC-7901 whole cell lysates, <br>Lane 2: rat testis tissue lysates,<br>Lane 3: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPMT antigen affinity purified polyclonal antibody (Catalog # A00671-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPMT at approximately 28KD. The expected band size for TPMT is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-trim5-picoband-trade-antibody-a01359-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01359-1-TRIM5-primary-antibodies-WB-testing-1.jpgAnti-TRIM5 Antibody Picoband® Western blot analysis of TRIM5 using anti-TRIM5 antibody (A01359-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human A375 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM5 antigen affinity purified polyclonal antibody (Catalog # A01359-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM5 at approximately 56KD. The expected band size for TRIM5 is at 56KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hausp-usp7-picoband-trade-antibody-a01239-2-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01239-2-usp7-primary-antibodies-wb-testing-1.jpgAnti-HAUSP/USP7 Antibody Picoband® Western blot analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat thymus tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUSP/USP7 antigen affinity purified polyclonal antibody (Catalog # A01239-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAUSP/USP7 at approximately 140 kDa. The expected band size for HAUSP/USP7 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01239-2-HAUSP-primary-antibodies-IHC-testing-2.jpgAnti-HAUSP/USP7 Antibody Picoband® IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2).
<br>HAUSP/USP7 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01239-2-HAUSP-primary-antibodies-IHC-testing-3.jpgAnti-HAUSP/USP7 Antibody Picoband® IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2).
<br>HAUSP/USP7 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01239-2-HAUSP-primary-antibodies-IHC-testing-4.jpgAnti-HAUSP/USP7 Antibody Picoband® IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2).
<br>HAUSP/USP7 was detected in paraffin-embedded section of mouse Lymph nodes tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01239-2-hausp-primary-antibodies-if-testing-5.jpg.jpgAnti-HAUSP/USP7 Antibody Picoband® IF analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2). <br> HAUSP/USP7 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01239-2-hausp-primary-antibodies-if-testing-6.jpg.jpgAnti-HAUSP/USP7 Antibody Picoband® IF analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (A01239-2). <br> HAUSP/USP7 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HAUSP/USP7 Antibody (A01239-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01239-2-hausp-primary-antibodies-fc-testing-7.pngAnti-HAUSP/USP7 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-HAUSP/USP7 antibody (A01239-2). <br> Overlay histogram showing A549 cells stained with A01239-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAUSP/USP7 Antibody (A01239-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-vapb-antibody-a01372-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-wb-testing-1_1.jpgAnti-VAPB Antibody Picoband® Western blot analysis of VAPB using anti-VAPB antibody (A01372).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human COLO-320 whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: human PANC-1 whole cell lysates,<br>
Lane 6: human A375 whole cell lysates,<br>
Lane 7: human Jurkat whole cell lysates,<br>
Lane 8: human placenta tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPB antigen affinity purified polyclonal antibody (Catalog # A01372) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27KD. The expected band size for VAPB is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-wb-testing-2.jpgAnti-VAPB Antibody Picoband® Western blot analysis of VAPB using anti-VAPB antibody (A01372).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: mouse liver tissue lysates, <br>
Lane 2: mouse heart tissue lysates, <br>
Lane 3: mouse skeletal muscle tissue lysates, <br>
Lane 4: mouse HEPA1-6 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPB antigen affinity purified polyclonal antibody (Catalog # A01372) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPB at approximately 27KD. The expected band size for VAPB is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-ihc-testing-3.jpgAnti-VAPB Antibody Picoband® IHC analysis of VAPB using anti-VAPB antibody (A01372). <br>
VAPB was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VAPB Antibody (A01372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-ihc-testing-4.jpgAnti-VAPB Antibody Picoband® IHC analysis of VAPB using anti-VAPB antibody (A01372). <br>
VAPB was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VAPB Antibody (A01372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-ihc-testing-5.jpgAnti-VAPB Antibody Picoband® IHC analysis of VAPB using anti-VAPB antibody (A01372). <br>
VAPB was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VAPB Antibody (A01372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-ihc-testing-6.jpgAnti-VAPB Antibody Picoband® IHC analysis of VAPB using anti-VAPB antibody (A01372). <br>
VAPB was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VAPB Antibody (A01372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-if-testing-7.jpgAnti-VAPB Antibody Picoband® IF analysis of VAPB using anti-VAPB antibody (A01372). <br>
VAPB was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-VAPB Antibody (A01372) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-fcm-testing-8.jpgAnti-VAPB Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-VAPB antibody (A01372).<br>Overlay histogram showing CACO-2 cells stained with A01372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VAPB Antibody (A01372,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01372-vapb-primary-antibodies-fcm-testing-9.jpgAnti-VAPB Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-VAPB antibody (A01372).<br>Overlay histogram showing U87 cells stained with A01372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VAPB Antibody (A01372,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-wwox-picoband-trade-antibody-a01223-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01223-1-wwox-primary-antibodies-wb-testing-1_2.jpgAnti-WWOX Antibody Picoband® Western blot analysis of WWOX using anti-WWOX antibody (A01223-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human COLO320 whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: human K562 whole cell lysates,<br>
Lane 6: human A549 whole cell lysates,<br>
Lane 7: human PC-3 whole cell lysates,<br>
Lane 8: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WWOX antigen affinity purified polyclonal antibody (Catalog # A01223-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WWOX at approximately 47 kDa. The expected band size for WWOX is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01223-1-wwox-primary-antibodies-wb-testing-2.jpgAnti-WWOX Antibody Picoband® Western blot analysis of WWOX using anti-WWOX antibody (A01223-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: rat PC-12 whole cell lysates,<br>
Lane 5: mouse testis tissue lysates,<br>
Lane 6: mouse kidney tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WWOX antigen affinity purified polyclonal antibody (Catalog # A01223-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WWOX at approximately 47 kDa. The expected band size for WWOX is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01223-1-WWOX-primary-antibodies-IHC-testing-3.jpgAnti-WWOX Antibody Picoband® IHC analysis of WWOX using anti-WWOX antibody (A01223-1).
<br>WWOX was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WWOX Antibody (A01223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01223-1-WWOX-primary-antibodies-IHC-testing-4.jpgAnti-WWOX Antibody Picoband® IHC analysis of WWOX using anti-WWOX antibody (A01223-1).
<br>WWOX was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WWOX Antibody (A01223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01223-1-WWOX-primary-antibodies-IHC-testing-5.jpgAnti-WWOX Antibody Picoband® IHC analysis of WWOX using anti-WWOX antibody (A01223-1).
<br>WWOX was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WWOX Antibody (A01223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01223-1-wwox-primary-antibodies-ihc-testing-6.jpgAnti-WWOX Antibody Picoband® IHC analysis of WWOX using anti-WWOX antibody (A01223-1).
<br>WWOX was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WWOX Antibody (A01223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01223-1-wwox-primary-antibodies-fc-testing-7.pngAnti-WWOX Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-WWOX antibody (A01223-1).<br> Overlay histogram showing U20S cells stained with A01223-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WWOX Antibody (A01223-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01223-1-wwox-primary-antibodies-if-testing-8.jpgAnti-WWOX Antibody Picoband® IF analysis of WWOX using anti-WWOX antibody (A01223-1). <br>
WWOX was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WWOX Antibody (A01223-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-14-3-3-zeta-delta-picoband-trade-antibody-a01141-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01141-1-ywhaz-primary-antibodies-wb-testing-1_1.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: human Raji whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: human A431 whole cell lysates,<br>
Lane 6: human PC-3 whole cell lysates,<br>
Lane 7: human MCF-7 whole cell lysates,<br>
Lane 8: human SiHa whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 zeta/delta antigen affinity purified polyclonal antibody (Catalog # A01141-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28 kDa. The expected band size for 14-3-3 zeta/delta is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01141-1-ywhaz-primary-antibodies-wb-testing-2_1.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: rat RH35 whole cell lysates,<br>
Lane 4: rat PC-12 whole cell lysates,<br>
Lane 5: mouse brain tissue lysates,<br>
Lane 6: mouse kidney tissue lysates,<br>
Lane 7: mouse ANA-1 whole cell lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 zeta/delta antigen affinity purified polyclonal antibody (Catalog # A01141-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28 kDa. The expected band size for 14-3-3 zeta/delta is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01141-1-ywhaz-primary-antibodies-if-testing-3.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® IF analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (A01141-1). <br>
14-3-3 zeta/delta was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-14-3-3 zeta/delta Antibody (A01141-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-brd7-picoband-trade-antibody-a01289-1-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01289-1-brd7-primary-antibodies-wb-testing-1.jpgAnti-BRD7 Antibody Picoband® Western blot analysis of BRD7 using anti-BRD7 antibody (A01289-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human CACO-2 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD7 antigen affinity purified polyclonal antibody (A01289-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD7 at approximately 85 kDa. The expected band size for BRD7 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01289-1-BRD7-primary-antibodies-IHC-testing-2.jpgAnti-BRD7 Antibody Picoband® IHC analysis of BRD7 using anti-BRD7 antibody (A01289-1).
<br>BRD7 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BRD7 Antibody (A01289-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01289-1-BRD7-primary-antibodies-IHC-testing-3.jpgAnti-BRD7 Antibody Picoband® IHC analysis of BRD7 using anti-BRD7 antibody (A01289-1).
<br>BRD7 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BRD7 Antibody (A01289-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/picokine-elisa-kits/mouse-ppbp-cxcl7-picokine-elisa-kit-ek0730-boster.html2026-03-24T05:16:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0730.pngMouse CXCL7/PPBP ELISA Kit PicoKine®Mouse CXCL7 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-ppbp-cxcl7-picokine-elisa-kit-ek0731-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0731.pngRat CXCL7/PPBP ELISA Kit PicoKine®Rat CXCL7 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-fgf23-picokine-elisa-kit-ek0759-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0759.pngHuman FGF23 ELISA Kit PicoKine®Human FGF23 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-plgf-2-picokine-elisa-kit-ek0863-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0863.pngMouse PLGF-2 / PGF ELISA Kit PicoKine®Mouse PLGF-2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-icos-picokine-elisa-kit-ek1111-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1111_1.pngHuman ICOS ELISA Kit PicoKine®Human ICOS PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-il-24-picokine-elisa-kit-ek1359-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1359.pngMouse IL-24/Interleukin-24 ELISA Kit PicoKine®Mouse IL-24 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-adipsin-cfd-picokine-elisa-kit-ek1420-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1420.jpgMouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®Mouse CFD PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-artemin-picokine-elisa-kit-ek1534-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1534.pngHuman Artemin ELISA Kit PicoKine®Human Artemin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-artemin-picokine-elisa-kit-ek1535-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1535.pngMouse Artemin ELISA Kit PicoKine®Mouse Artemin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-bikunin-picokine-elisa-kit-ek1598-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1598.pngHuman Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine®Human Bikunin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-a5-pai3-picokine-elisa-kit-ek1631-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1631.pngHuman Serpin A5/PAI3 ELISA Kit PicoKine®Human Serpin A5 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd209-picokine-elisa-kit-ek1632-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1632.jpgHuman CD209 ELISA Kit PicoKine®Human CD209 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-pla2g7-lp-pla2-picokine-elisa-kit-ek1637-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1637.jpgHuman PLA2G7/Lp-PLA2 ELISA Kit PicoKine®Human PLA2G7 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-hyaluronidase1-hyal1-picokine-elisa-kit-ek1639-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1639.pngHuman Hyaluronidase1/HYAL1 ELISA Kit PicoKine®Human HYAL1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-tim-3-havcr2-picokine-elisa-kit-ek1644-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1644.pngHuman TIM-3/HAVCR2 ELISA Kit PicoKine®Human TIM-3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-tim-3-havcr2-picokine-elisa-kit-ek1645-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1645.pngMouse TIM-3/HAVCR2 ELISA Kit PicoKine®Mouse TIM-3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-vap-1-aoc3-picokine-elisa-kit-ek1646-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1646.jpgHuman VAP-1/AOC3 ELISA Kit PicoKine®Human VAP-1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-cxcl4-pf4-picokine-elisa-kit-ek1651-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1651.pngRat CXCL4/PF4 ELISA Kit PicoKine®Rat CXCL4 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-il-12-p70-picokine-elisa-kit-ek1652-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1652.pngRat IL-12(p70) ELISA Kit PicoKine®Rat IL-12(p70) PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/anti-annexin-vi-picoband-trade-antibody-a03735-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-wb-testing-1.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat liver tissue lysates,<br>
Lane 6: rat lung tissue lysates,<br>
Lane 7: mouse liver tissue lysates,<br>
Lane 8: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-ihc-testing-2.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® IHC analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Annexin VI/ANXA6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin VI/ANXA6 Antibody (A03735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-ihc-testing-3.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® IHC analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Annexin VI/ANXA6 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin VI/ANXA6 Antibody (A03735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-ihc-testing-4.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® IHC analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Annexin VI/ANXA6 was detected in a paraffin-embedded section of mouse lymphade tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin VI/ANXA6 Antibody (A03735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-ihc-testing-5.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® IHC analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Annexin VI/ANXA6 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin VI/ANXA6 Antibody (A03735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03735-anxa6-primary-antibodies-fcm-testing-6.jpgAnti-Annexin VI/ANXA6 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Annexin VI/ANXA6 antibody (A03735). <br>
Overlay histogram showing HL-60 cells stained with A03735 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin VI/ANXA6 Antibody (A03735, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-betacellulin-picoband-trade-antibody-a02171-1-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02171-1-Betacellulin-primary-antibodies-WB-testing-1.jpgAnti-Betacellulin/Btc Antibody Picoband® Western blot analysis of Betacellulin using anti-Betacellulin antibody (A02171-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat PC-12 whole cell lysates,<br>Lane 2: rat RH35 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Betacellulin antigen affinity purified polyclonal antibody (Catalog # A02171-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Betacellulin at approximately 26KD. The expected band size for Betacellulin is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02171-1-Betacellulin-primary-antibodies-IHC-testing-2.jpgAnti-Betacellulin/Btc Antibody Picoband® IHC analysis of Betacellulin using anti-Betacellulin antibody (A02171-1).
<br>Betacellulin was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Betacellulin Antibody (A02171-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02171-1-Betacellulin-primary-antibodies-IHC-testing-3.jpgAnti-Betacellulin/Btc Antibody Picoband® IHC analysis of Betacellulin using anti-Betacellulin antibody (A02171-1).
<br>Betacellulin was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Betacellulin Antibody (A02171-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02171-1-Betacellulin-primary-antibodies-IHC-testing-4.jpgAnti-Betacellulin/Btc Antibody Picoband® IHC analysis of Betacellulin using anti-Betacellulin antibody (A02171-1).
<br>Betacellulin was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Betacellulin Antibody (A02171-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02171-1-Betacellulin-primary-antibodies-IHC-testing-5.jpgAnti-Betacellulin/Btc Antibody Picoband® IHC analysis of Betacellulin using anti-Betacellulin antibody (A02171-1).
<br>Betacellulin was detected in paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Betacellulin Antibody (A02171-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-ccr4-picoband-trade-antibody-a00755-boster.html2026-03-24T05:16:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00755-CCR4-primary-antibodies-WB-testing-1.jpgAnti-CCR4 Antibody Picoband® Western blot analysis of CCR4 using anti-CCR4 antibody (A00755).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat thymus tissue lysates,<br>Lane 2: mouse thymus tissue lysates,<br>Lane 3: mouse NIH3T3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR4 antigen affinity purified polyclonal antibody (Catalog # A00755) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR4 at approximately 41KD. The expected band size for CCR4 is at 41KD.
https://www.bosterbio.com/products/primary-antibodies/anti-chrna3-picoband-trade-antibody-a01981-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01981-1-chrna3-primary-antibodies-wb-testing-1.jpgAnti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®Western blot analysis of CHRNA3 using anti-CHRNA3 antibody (A01981-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA3 antigen affinity purified polyclonal antibody (A01981-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CHRNA3 at approximately 50-55 kDa. The expected band size for CHRNA3 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01981-1-chrna3-primary-antibodies-fcm-testing-1.jpgAnti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-CHRNA3 antibody (A01981-1). <br>
Overlay histogram showing A549 cells stained with A01981-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA3 Antibody (A01981-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cox-iv-picoband-trade-antibody-a05442-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-1-cox_iv-primary-antibodies-wb-testing-1.jpgAnti-COX IV/COX4I1 Antibody Picoband® Western blot analysis of COX IV using anti-COX IV antibody (A05442-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: human SiHa whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody (Catalog # A05442-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX IV at approximately 17 kDa. The expected band size for COX IV is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-1-cox_iv-primary-antibodies-ihc-testing-2.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-3.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-4.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-5.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-6.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-7.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-8.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05442-1-COX_IV-primary-antibodies-IHC-testing-9.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV using anti-COX IV antibody (A05442-1).
<br>COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-1-cox_iv-primary-antibodies-if-testing-10.jpgAnti-COX IV/COX4I1 Antibody Picoband® IF analysis of COX IV using anti-COX IV antibody (A05442-1). <br>
COX IV was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-COX IV Antibody (A05442-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-1-cox_iv-primary-antibodies-fcm-testing-11.jpgAnti-COX IV/COX4I1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-COX IV antibody (A05442-1).<br>Overlay histogram showing U937 cells stained with A05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX IV Antibody (A05442-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cathepsin-d-picoband-trade-antibody-a01361-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01361-Cathepsin_D-primary-antibodies-WB-testing-1.jpgAnti-Cathepsin D/Ctsd Antibody Picoband® Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (A01361).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse testis tissue lysates,<br>Lane 2: rat testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hours at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # A01361) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times for 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 45KD. The expected band size for Cathepsin D is at 45KD.
https://www.bosterbio.com/products/primary-antibodies/anti-factor-v-antibody-a00440-1-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00440-1-factor_v-primary-antibodies-wb-testing-1.jpgAnti-Factor V/F5 Antibody Picoband® Western blot analysis of Factor V using anti-Factor V antibody (A00440-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human plasma lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor V antigen affinity purified polyclonal antibody (Catalog # A00440-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor V at approximately 252KD. The expected band size for Factor V is at 252KD.
https://www.bosterbio.com/products/primary-antibodies/anti-liver-fabp-picoband-trade-antibody-a02477-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02477-liver-fabp-primary-antibodies-wb-testing-1.jpgAnti-liver FABP/Fabp1 Antibody Picoband® Western blot analysis of Liver FABP/Fabp1 using anti-Liver FABP/Fabp1 antibody (A02477). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
Lane 4: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Liver FABP/Fabp1 antigen affinity purified polyclonal antibody (Catalog # A02477) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Liver FABP/Fabp1 at approximately 14 kDa. The expected band size for Liver FABP/Fabp1 is at 14 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-fabp5-picoband-trade-antibody-a02083-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02083-fabp5-primary-antibodies-wb-testing-1.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband® Western blot analysis of FABP5 using anti-FABP5 antibody (A02083). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human RT4 whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: rat lung tissue lysates,<br>
Lane 4: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP5 antigen affinity purified polyclonal antibody (Catalog # A02083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15 kDa. The expected band size for FABP5 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02083-fabp5-primary-antibodies-if-testing-2.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband® IF analysis of FABP5 using anti-FABP5 antibody (A02083). <br>
FABP5 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FABP5 Antibody (A02083) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-fgr-picoband-trade-antibody-a01674-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01674-1-FGR-primary-antibodies-WB-testing-1.jpgAnti-FGR Antibody Picoband® Western blot analysis of FGR using anti-FGR antibody (A01674-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SW579 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGR antigen affinity purified polyclonal antibody (Catalog # A01674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGR at approximately 59KD. The expected band size for FGR is at 59KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fra1-picoband-trade-antibody-a03927-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03927-1-FRA1-primary-antibodies-WB-testing-1.jpgAnti-FRA1/FOSL1 Antibody Picoband® Western blot analysis of FRA1 using anti-FRA1 antibody (A03927-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human A375 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified polyclonal antibody (Catalog # A03927-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 29KD. The expected band size for FRA1 is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-gadd45g-antibody-a04681-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04681-1-GADD45G-primary-antibodies-WB-testing-2.jpgAnti-GADD45G Antibody Picoband® Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat heart tissue lysates,<br>Lane 3: rat testis tissue lysates,<br>Lane 4: rat skeletal muscle tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse heart tissue lysates,<br>Lane 7: mouse testis tissue lysates,<br>Lane 8: mouse skeletal muscle tissue lysates,
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04681-1-GADD45G-primary-antibodies-WB-testing-1.jpgAnti-GADD45G Antibody Picoband® Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human SW620 whole cell lysates,<br>Lane 4: human A549 whole cell lysates,<br>Lane 5: mouse NIH3T3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hdac10-antibody-a05215-2-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05215-2-HDAC10-primary-antibodies-WB-testing-1.jpgAnti-HDAC10 Antibody Picoband® Western blot analysis of HDAC10 using anti-HDAC10 antibody (A05215-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human MDA-MB-453 whole cell lysates,<br>Lane 2: human SW620 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC10 antigen affinity purified polyclonal antibody (Catalog # A05215-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC10 at approximately 71KD. The expected band size for HDAC10 is at 71KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hgd-picoband-trade-antibody-a01909-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01909-1-HGD-primary-antibodies-WB-testing-1.jpgAnti-HGD Antibody Picoband® Western blot analysis of HGD using anti-HGD antibody (A01909-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates,<br>Lane 2: human A549 whole cell lysates,<br>Lane 3: rat liver tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: mouse liver tissue lysates,<br>Lane 6: mouse kidney tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGD antigen affinity purified polyclonal antibody (Catalog # A01909-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGD at approximately 50KD. The expected band size for HGD is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01909-1-HGD-primary-antibodies-IHC-testing-2.jpgAnti-HGD Antibody Picoband® IHC analysis of HGD using anti-HGD antibody (A01909-1).
<br>HGD was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HGD Antibody (A01909-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01909-1-HGD-primary-antibodies-IHC-testing-3.jpgAnti-HGD Antibody Picoband® IHC analysis of HGD using anti-HGD antibody (A01909-1).
<br>HGD was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HGD Antibody (A01909-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01909-1-hgd-primary-antibody-if-testing-4.jpgAnti-HGD Antibody Picoband® IF analysis of HGD using anti-HGD antibody (A01909-1). <br>
HGD was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HGD Antibody (A01909-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01909-1-hgd-primary-antibody-fcm-testing-5.pngAnti-HGD Antibody Picoband® Flow Cytometry analysis of HEPG2 cells using anti-HGD antibody (A01909-1). <br>Overlay histogram showing HEPG2 cells stained with A01909-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HGD Antibody (A01909-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hlf-picoband-trade-antibody-a01284-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01284-1-hlf-primary-antibodies-wb-testing-1_1.jpgAnti-HLF Antibody Picoband®Western blot analysis of HLF using anti-HLF antibody (A01284-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (A01284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLF at approximately 35 kDa. The expected band size for HLF is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01284-1-hlf-primary-antibodies-if-testing-1.jpgAnti-HLF Antibody Picoband®IF analysis of HLF using anti-HLF antibody (A01284-1). <br>
HLF was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HLF Antibody (A01284-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01284-1-hlf-primary-antibodies-fcm-testing-1.jpgAnti-HLF Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-HLF antibody (A01284-1). <br>
Overlay histogram showing HepG2 cells stained with A01284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLF Antibody (A01284-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hmbs-picoband-trade-antibody-a01506-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02674-2-hmbs-primary-antibodies-wb-testing-1.jpgAnti-HMBS Antibody Picoband®Western blot analysis of HMBS using anti-HMBS antibody (A01506-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human CACO-2 whole cell lysates, <br>
Lane 5: rat spleen tissue lysates, <br>
Lane 6: mouse spleen tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody (A01506-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMBS at approximately 45 kDa. The expected band size for HMBS is at 39 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-a2b1-picoband-trade-antibody-a00396-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnpa2b1-primary-antibodies-wb-testing-1.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® Western blot analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: human SH-SY5Y whole cell lysates,<br>
Lane 6: human U2OS whole cell lysates,<br>
Lane 7: human HEL whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP A2B1 antigen affinity purified polyclonal antibody (Catalog # A00396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP A2B1 at approximately 37 kDa. The expected band size for hnRNP A2B1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnp_a2b1-primary-antibodies-ihc-testing-2.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). <br>
hnRNP A2B1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnp_a2b1-primary-antibodies-ihc-testing-3.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). <br>
hnRNP A2B1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnp_a2b1-primary-antibodies-ihc-testing-4.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). <br>
hnRNP A2B1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnp_a2b1-primary-antibodies-if-testing-5.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® IF analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1). <br>
hnRNP A2B1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00396-1-hnrnp_a2b1-primary-antibodies-fcm-testing-6.jpgAnti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-hnRNP A2B1 antibody (A00396-1).<br>Overlay histogram showing A431 cells stained with A00396-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP A2B1 Antibody (A00396-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-il10-picoband-trade-antibody-a00021-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-il10-primary-antibodies-wb-testing-1.jpgAnti-IL10 Antibody Picoband® Western blot analysis of IL10 using anti-IL10 antibody (A00021). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse brain tissue lysates,<br>Lane 2: mouse spleen tissue lysates,<br>Lane 3: mouse heart tissue lysates,<br>Lane 4: mouse liver tissue lysates,<br>Lane 5: mouse kidney tissue lysates,<br>Lane 6: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-fpsyt-09-00434-g006.jpgAnti-IL10 Antibody Picoband®Effect of fluoxetine treatment on BDNF and inflammation levels. (A) Western blot results of NF-κB, IL-10, iNOS, BDNF, and GADPH. Quantification analysis of (B) NF-κB, (C) iNOS, (D) IL-10, and (E) BDNF. Values are expressed as mean ± SEM ( n > 5). N+C+P-FLX vs. N+C+P-con, * P < 0.05, ** P < 0.01, *** P < 0.001; N+C+P-con vs. N+P, * P < 0.05; A+C+P-FLX vs. A+C+P-con, * P < 0.05, *** P < 0.001; A+C+P-con vs. A+P, * P < 0.05; N+C+P+FLX vs. A+C+P-FLX, ∧∧ P < 0.01; N+C+P-con vs. A+C+P-con, && P < 0.01; N+P vs. A+P, # P < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/psychiatry/articles/10.3389/fpsyt.2018.00434/full'>30349488</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-il10-primary-antibodies-elisa-testing-2.jpgAnti-IL10 Antibody Picoband® Sandwich ELISA - Recombinant mouse IL10 protein standard curve.<br>
Use in combination with reagents from Mouse IL10 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0417).
https://www.bosterbio.com/products/primary-antibodies/anti-il27-picoband-trade-antibody-a00857-2-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00857-2-IL27-primary-antibodies-WB-testing-1.jpgAnti-IL27 Antibody Picoband® Western blot analysis of IL27 using anti-IL27 antibody (A00857-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat NRK whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL27 antigen affinity purified polyclonal antibody (Catalog # A00857-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL27 at approximately 29KD. The expected band size for IL27 is at 27KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ship-picoband-trade-antibody-a03358-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03358-SHIP-primary-antibodies-WB-testing-1.jpgAnti-SHIP/INPP5D Antibody Picoband® Western blot analysis of SHIP using anti-SHIP antibody (A03358).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human CCRF-CEM whole cell lysates,<br>Lane 2: human SW620 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHIP antigen affinity purified polyclonal antibody (Catalog # A03358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHIP at approximately 145KD. The expected band size for SHIP is at 133KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03358-SHIP-primary-antibodies-IHC-testing-2.jpgAnti-SHIP/INPP5D Antibody Picoband® IHC analysis of SHIP using anti-SHIP antibody (A03358).
<br>SHIP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03358-SHIP-primary-antibodies-IHC-testing-3.jpgAnti-SHIP/INPP5D Antibody Picoband® IHC analysis of SHIP using anti-SHIP antibody (A03358).
<br>SHIP was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03358-SHIP-primary-antibodies-IHC-testing-4.jpgAnti-SHIP/INPP5D Antibody Picoband® IHC analysis of SHIP using anti-SHIP antibody (A03358).
<br>SHIP was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SHIP Antibody (A03358) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-jund-picoband-trade-antibody-a05609-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05609-1-jund-primary-antibodies-wb-testing-1.jpgAnti-JunD Antibody Picoband® Western blot analysis of JunD using anti-JunD antibody (A05609-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: human 293T whole cell lysates,<br>
Lane 6: human SH-SY5Y whole cell lysates,<br>
Lane 7: human U2OS whole cell lysates,<br>
Lane 8: human U251 whole cell lysates,<br>
Lane 9: rat lung tissue lysates,<br>
Lane 10: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JunD antigen affinity purified polyclonal antibody (Catalog # A05609-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JunD at approximately 38 kDa, 42 kDa. The expected band size for JunD is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05609-1-jund-primary-antibodies-ihc-testing-2.jpgAnti-JunD Antibody Picoband® IHC analysis of JunD using anti-JunD antibody (A05609-1). <br>
JunD was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JunD Antibody (A05609-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05609-1-jund-primary-antibodies-ihc-testing-3.jpgAnti-JunD Antibody Picoband® IHC analysis of JunD using anti-JunD antibody (A05609-1). <br>
JunD was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JunD Antibody (A05609-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05609-1-jund-primary-antibodies-fcm-testing-4.jpgAnti-JunD Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-JunD antibody (A05609-1). <br>
Overlay histogram showing K562 cells stained with A05609-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JunD Antibody (A05609-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lgals3bp-picoband-trade-antibody-a02938-1-boster.html2026-03-24T05:16:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02938-1-lgals3bp-primary-antibodies-wb-testing-1_1.jpgAnti-LGALS3BP Antibody Picoband® Western blot analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS3BP antigen affinity purified polyclonal antibody (Catalog # A02938-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LGALS3BP at approximately 75 kDa. The expected band size for LGALS3BP is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02938-1-LGALS3BP-primary-antibodies-IHC-testing-2.jpgAnti-LGALS3BP Antibody Picoband® IHC analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1).
<br>LGALS3BP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LGALS3BP Antibody (A02938-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02938-1-LGALS3BP-primary-antibodies-IHC-testing-3.jpgAnti-LGALS3BP Antibody Picoband® IHC analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1).
<br>LGALS3BP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LGALS3BP Antibody (A02938-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02938-1-LGALS3BP-primary-antibodies-IHC-testing-4.jpgAnti-LGALS3BP Antibody Picoband® IHC analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1).
<br>LGALS3BP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LGALS3BP Antibody (A02938-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02938-1-LGALS3BP-primary-antibodies-IHC-testing-5.jpgAnti-LGALS3BP Antibody Picoband® IHC analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1).
<br>LGALS3BP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LGALS3BP Antibody (A02938-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02938-1-LGALS3BP-primary-antibodies-IHC-testing-6.jpgAnti-LGALS3BP Antibody Picoband® IHC analysis of LGALS3BP using anti-LGALS3BP antibody (A02938-1).
<br>LGALS3BP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LGALS3BP Antibody (A02938-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-lmo2-picoband-trade-antibody-a03502-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03502-1-LMO2-primary-antibodies-WB-testing-1.jpgAnti-LMO2 Antibody Picoband® Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysates,<br>Lane 2: human SMMC-7721 whole cell lysates,<br>Lane 3: human A375 whole cell lysates,<br>Lane 4: human A431 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03502-1-LMO2-primary-antibodies-WB-testing-2.jpgAnti-LMO2 Antibody Picoband® Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat C6 whole cell lysates,<br>Lane 2: mouse Neuro-2a whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mbl2-picoband-trade-antibody-a01000-3-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01000-3-mbl2-primary-antibodies-wb-testing-1.jpgAnti-MBL2 Antibody Picoband® Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse liver tissue lysates,<br>
Lane 2: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01000-3-mbl2-primary-antibodies-elisa-testing-2.jpgAnti-MBL2 Antibody Picoband® Sandwich ELISA - Recombinant mouse MBL2 protein standard curve.<br>
Use in combination with reagents from Mouse MBL2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0806).
https://www.bosterbio.com/products/primary-antibodies/anti-muc7-picoband-trade-antibody-a05210-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05210-MUC7-primary-antibodies-WB-testing-1.jpgAnti-MUC7 Antibody Picoband® Western blot analysis of MUC7 using anti-MUC7 antibody (A05210).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SW620 whole cell lysates,<br>Lane 2: human HepG2 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC7 antigen affinity purified polyclonal antibody (Catalog # A05210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC7 at approximately 46KD. The expected band size for MUC7 is at 39KD.
https://www.bosterbio.com/products/primary-antibodies/anti-nedd4-picoband-trade-antibody-a00984-2-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-2-nedd4-primary-antibodies-wb-testing-1_1.jpgAnti-NEDD4 Antibody Picoband®Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-2). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 5: human U251 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (A00984-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEDD4 at approximately 147 kDa. The expected band size for NEDD4 is at 147 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-niemann-pick-c1-picoband-trade-antibody-a00428-2-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00428-2-niemann_pick_c1-primary-antibodies-wb-testing-1.jpgAnti-Niemann Pick C1/NPC1 Antibody Picoband® Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody (A00428-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat pancreas tissue lysates,<br>Lane 2: mouse NIH3T3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody (Catalog # A00428-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.
https://www.bosterbio.com/products/primary-antibodies/anti-niemann-pick-c2-antibody-a01582-2-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-2-npc2-primary-antibodies-wb-testing-1.jpgAnti-Niemann Pick C2/NPC2 Antibody Picoband®Western blot analysis of NPC2 using anti-NPC2 antibody (A01582-2). <br>
Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPC2 antigen affinity purified polyclonal antibody (A01582-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPC2 at approximately 16-19 kDa. The expected band size for NPC2 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-2-npc2-primary-antibodies-ihc-testing-1.jpgAnti-Niemann Pick C2/NPC2 Antibody Picoband®IHC analysis of NPC2 using anti-NPC2 antibody (A01582-2). <br>
NPC2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPC2 Antibody (A01582-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-trka-picoband-trade-antibody-a00706-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00706-TrkA-primary-antibodies-WB-testing-1.jpgAnti-TrkA/NTRK1 Antibody Picoband® Western blot analysis of TrkA using anti-TrkA antibody (A00706).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human SGC-7901 whole cell lysates,<br>Lane 3: human THP-1 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkA antigen affinity purified polyclonal antibody (Catalog # A00706) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkA at approximately 150KD. The expected band size for TrkA is at 87KD.
https://www.bosterbio.com/products/primary-antibodies/anti-oif-picoband-trade-antibody-a07061-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07061-1-OIF-primary-antibodies-WB-testing-1.jpgAnti-OIF/Ogn Antibody Picoband® Western blot analysis of OIF using anti-OIF antibody (A07061-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
<br>Lane 1: recombinant mouse OGN protein 1ng.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIF antigen affinity purified polyclonal antibody (Catalog # A07061-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIF at approximately 34KD. The expected band size for OIF is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07061-1-13048_2024_1364_fig3_html.pngAnti-OIF/Ogn Antibody Picoband®Culture with OGN-overexpressing CAFs-derived conditioned medium inhibits ovarian cancer cell aggressiveness ( A – B ) The mRNA expression and protein levels of OGN were examined in CAFs and NFs by qRT-PCR and Immunoblotting, respectively. ( C – D ) SKOV3 and A2780 cells were co-cultured with CAFs- or NFs-derived conditioned medium (CAFs-CM/NFs-CM) and examined for the mRNA expression and protein levels of OGN in SKOV3 and A2780 cells by qRT-PCR and Immunoblotting, respectively. ( E – F ) OGN overexpression was achieved in CAFs by transducing OGN overexpression vector (OGN-OE); OGN overexpression was confirmed by qRT-PCR and Immunoblotting, respectively. Then, SKOV3 and A2780 cells were cultured in control medium (con-CM), CAFs-CM, NF-CM, CAFs (lv-NC)-CM, or CAFs (OGN-OE)-CM, and examined for cell viability by MTT assay ( G ); DNA synthesis capacity by EdU ( H ); cell invasion by Transwell with chambers pre-coated with Matrigel ( I ); the protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt by Immunoblotting ( J ). ** P < 0.01, compared with NFs or CAFs (NC) group; ## P < 0.01 compared with CAFs (NC)-CM group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-024-01364-w'>38402185</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07061-1-13048_2024_1364_fig2_html.pngAnti-OIF/Ogn Antibody Picoband®miR-1290 directly targets OGN and inhibits OGN expression ( A ) Targetscan, miRDIP, and literature search methods were used to search for miRNAs that might target OGN and serve as oncogenic miRNAs, and two miRNAs (miR-223-3p and miR-1290) were obtained. ( B ) The expression of miR-223-3p and miR-1290was examined in CAFs and NFs by qRT-PCR. ( C – D ) miR-1290 overexpression or inhibition was achieved in CAFs by transducing miR-1290 mimics or inhibitor; miR-1290 overexpression or inhibition was confirmed by qRT-PCR and Immunoblotting, respectively. ( E ) CAFs were transduced with miR-1290 mimics or inhibitor and examined for the mRNA expression by qRT-PCR. ( F ) Wild- and mutant-type OGN luciferase reporter vectors (wt-OGN/mut-OGN) were constructed as described in the M&M section. Then, wt-OGN or mut-OGN was co-transduced into 293T cells with miR-1290 mimics/inhibitor; the luciferase activity was determined. ( G ) The expression and location of miR-1290 and OGN in CAFs and NFs were determined by FISH assay. miR-1290 (green), OGN (red). Scale bar = 20 μm. * P < 0.05, ** P < 0.01, compared with NC mimics; ## P < 0.01, compared with NC inhibitor <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-024-01364-w'>38402185</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07061-1-13048_2024_1364_fig5_html.pngAnti-OIF/Ogn Antibody Picoband®The miR-1290/OGN axis in CAFs modulates ovarian cancer cell aggressiveness SKOV3 and A2780 cells were cultured in CAFs co-transduced with miR-1290 mimics and OGN-OE and examined for cell viability by MTT ( A ); DNA synthesis capacity by EdU ( B ); cell invasion by Transwell with chambers pre-coated with Matrigel ( C ); the protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt by Immunoblotting ( D ). ** P < 0.01, compared with CAFs (NC + NC mimics)-CM group; ## P < 0.01 compared with CAFs (OGN-OE + miR-1290 mimics)-CM group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-024-01364-w'>38402185</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07061-1-13048_2024_1364_fig6_html.pngAnti-OIF/Ogn Antibody Picoband®The miR-1290/OGN axis in CAFs modulates tumorigenic capacity of ovarian cancer cell in vivo Xenograft transplanted tumor models were established in nude mice by injecting a mixture of SKOV3 cells with CAFs or infected CAFs (infected with miR-1290 mimics or lv-OGN) and nude mice were separated into five groups ( n = 6 per group): the SKOV3 cells + CAFs group, the SKOV3 cells + CAFs (lv-NC + NC mimics) group, the SKOV3 cells + CAFs (lv-OGN + NC mimics) group, the SKOV3 cells + CAFs (lv-NC + miR-1290 mimics) group, the SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group. ( A ) Images of the tumors in each group. ( B ) Tumor volumes were measured every five days from the 10th day of the experiment. ( C ) Tumor weight were determined at the 30th day. ( D – E ) The mRNA levels of OGN and miR-1290 in mice tumor tissues were detected by qRT-PCR. ( F ) Ki67 and Vimentin levels in tumor tissues were examined using IHC staining. Scale bar = 20 μm. ( G ) The protein levels of E-cadherin, vimentin, p-mTOR, mTOR, p-Akt, and Akt in tumor tissues were determined using immunoblotting. * P < 0.05, ** P < 0.01, compared between SKOV3 cells + CAFs group and SKOV3 cells + CAFs (lv-NC + NC mimics); ## P < 0.01 compared with SKOV3 cells + CAFs (lv-NC + NC mimics) group; && P < 0.01 compared with SKOV3 cells + CAFs (lv-OGN + miR-1290 mimics) group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-024-01364-w'>38402185</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07061-1-oif-primary-antibodies-elisa-testing-2.jpgAnti-OIF/Ogn Antibody Picoband® Sandwich ELISA - Recombinant mouse OIF/Ogn protein standard curve.<br>
Use in combination with reagents from Mouse OIF/Ogn ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1617).
https://www.bosterbio.com/products/primary-antibodies/anti-plcg-2-picoband-trade-antibody-a03572-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03572-1-plcg2-primary-antibodies-fcm-testing-4.pngAnti-PLCG 2/PLCG2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PLCG 2/PLCG2 antibody (A03572-1). <br>Overlay histogram showing THP-1 cells stained with A03572-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03572-1-plcg2-primary-antibodies-wb-testing-1.jpgAnti-PLCG 2/PLCG2 Antibody Picoband® Western blot analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: human Daudi whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG 2/PLCG2 antigen affinity purified polyclonal antibody (Catalog # A03572-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCG 2/PLCG2 at approximately 148 kDa. The expected band size for PLCG 2/PLCG2 is at 148 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03572-1-plcg2-primary-antibodies-ihc-testing-2.jpgAnti-PLCG 2/PLCG2 Antibody Picoband® IHC analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1). <br>
PLCG 2/PLCG2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03572-1-plcg2-primary-antibodies-if-testing-3.jpgAnti-PLCG 2/PLCG2 Antibody Picoband® IF analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1). <br>
PLCG 2/PLCG2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLCG 2/PLCG2 Antibody (A03572-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-protein-c-antibody-a01742-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01742-protein_c-primary-antibodies-wb-testing-1.jpgAnti-Protein C/PROC Antibody Picoband® Western blot analysis of Protein C using anti-Protein C antibody (A01742). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates,<br>Lane 2: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # A01742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
https://www.bosterbio.com/products/primary-antibodies/anti-psd-picoband-trade-antibody-a01427-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01427-psd-primary-antibodies-wb-testing-1.jpgAnti-PSD Antibody Picoband® Western blot analysis of PSD using anti-PSD antibody (A01427). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD antigen affinity purified polyclonal antibody (Catalog # A01427) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD at approximately 130KD. The expected band size for PSD is at 109KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rab6a-picoband-trade-antibody-a02911-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02911-1-RAB6A-primary-antibodies-WB-testing-1.jpgAnti-RAB6A Antibody Picoband® Western blot analysis of RAB6A using anti-RAB6A antibody (A02911-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human MDA-MB-453 whole cell lysates,<br>Lane 2: human SK-OV-3 whole cell lysates,<br>Lane 3: Human A431 whole cell lysates,<br>Lane 4: rat testis tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB6A antigen affinity purified polyclonal antibody (Catalog # A02911-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB6A at approximately 24KD. The expected band size for RAB6A is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02911-1-rab6a-primary-antibodies-ihc-testing-2.jpgAnti-RAB6A Antibody Picoband® IHC analysis of RAB6A using anti-RAB6A antibody (A02911-1).<br> RAB6A was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-RAB6A Antibody (A02911-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02911-1-3.jpgAnti-RAB6A Antibody Picoband® IHC analysis of RAB6A using anti-RAB6A antibody (A02911-1). <br> RAB6A was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB6A Antibody (A02911-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf43-picoband-trade-antibody-a01694-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01694-1-rnf43-primary-antibodies-wb-testing-1_1.jpgAnti-RNF43 Antibody Picoband®Western blot analysis of RNF43 using anti-RNF43 antibody (A01694-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF43 antigen affinity purified polyclonal antibody (A01694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RNF43 at approximately 86 kDa. The expected band size for RNF43 is at 86 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-spr-picoband-trade-antibody-a00416-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00416-1-SPR-primary-antibodies-WB-testing-1.jpgAnti-SPR Antibody Picoband® Western blot analysis of SPR using anti-SPR antibody (A00416-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysates,<br>Lane 2: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPR antigen affinity purified polyclonal antibody (Catalog # A00416-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPR at approximately 28KD. The expected band size for SPR is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-star-picoband-trade-antibody-a00051-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00051-1-star-primary-antibodies-wb-testing-1.jpgAnti-StAR Antibody Picoband® Western blot analysis of StAR using anti-StAR antibody (A00051-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rank-antibody-a01064-2-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01064-2-RANK-primary-antibodies-WB-testing-1.jpgAnti-RANK/TNFRSF11A Antibody Picoband® Western blot analysis of RANK using anti-RANK antibody (A01064-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human MCF-7 whole cell lysates,<br>Lane 4: human SK-OV-3 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANK antigen affinity purified polyclonal antibody (Catalog # A01064-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD.
https://www.bosterbio.com/products/primary-antibodies/anti-tryptophan-hydroxylase-picoband-trade-antibody-a01626-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01626-1-Tryptophan_Hydroxylase-primary-antibodies-WB-testing-1.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SGC-7901 whole cell lysates,<br>Lane 2: rat heart tissue lysates,<br>Lane 3: mouse heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-1-tryptophan_hydroxylase-primary-antibodies-wb-testing-2.pngAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: normal group-rat colon tissue lysates,<br>
Lane 2: control group-model rat colon tissue lysates,<br>
Lane 3: low dose Chinese medicine group-model rat colon tissue lysates,<br>
Lane 4: medium dose Chinese medicine group-model rat colon tissue lysates,<br>
Lane 5: high dose Chinese medicine group-model rat colon tissue lysates,<br>
Lane 6: western medicine group-model rat colon tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. The expected band size for Tryptophan Hydroxylase is at 51KD.
https://www.bosterbio.com/products/primary-antibodies/anti-vegf-picoband-trade-antibody-a00045-1-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00045-1-VEGF-primary-antibodies-WB-testing-1.jpgAnti-VEGF/Vegfa/VEGF164 Antibody Picoband® Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse thymus tissue lysates,<br>Lane 2: mouse HEPA1-6 whole cell lysates,<br>Lane 3: rat C6 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
https://www.bosterbio.com/products/primary-antibodies/anti-lymphotactin-picoband-trade-antibody-a04548-boster.html2026-03-24T05:16:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04548-Lymphotactin-primary-antibodies-WB-testing-1.jpgAnti-Lymphotactin/Xcl1 Antibody Picoband® Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (A04548).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse spleen tissue lysates,<br>Lane 2: mouse kidney tissue lysates,<br>Lane 3: rat kidney tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # A04548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 17KD. The expected band size for Lymphotactin is at 12KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-iv-picoband-trade-antibody-m01411-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01411-Collagen_IV-primary-antibodies-IHC-testing-1_1.jpgAnti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411).<br> Collagen IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01411-Collagen_IV-primary-antibodies-IHC-testing-2_1.jpgAnti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411).<br> Collagen IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01411-collagen-iv-primary-antibodies-wb-testing-3_2.jpgAnti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3) Western blot analysis of Collagen IV using anti-Collagen IV antibody (M01411). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293T whole cell lysate,<br> Lane 2: human Hela whole cell lysate,<br> Lane 3: human A549 whole cell lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen IV antigen affinity purified monoclonal antibody (Catalog # M01411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 220KD. The expected band size for Collagen IV is at 161KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tcp1-alpha-picoband-trade-antibody-m02389-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02389-TCP1_alpha-primary-antibodies-WB-testing-1_1.jpgAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) Western blot analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human COLO-320 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: human A431 whole cell lysates, <br> Lane 6: human HT1080 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TCP1 alpha antigen affinity purified monoclonal antibody (Catalog # M02389) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02389-TCP1_alpha-primary-antibodies-IHC-testing-2_1.jpgAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). <br> TCP1 alpha was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-TCP1 alpha Antibody (M02389) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02389-TCP1_alpha-primary-antibodies-IHC-testing-3_1.jpgAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). <br> TCP1 alpha was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-TCP1 alpha Antibody (M02389) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02389-4.pngAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) Flow Cytometry analysis of HepG2 cells using anti-TCP1 alpha antibody (M02389). <br> Overlay histogram showing HepG2 cells stained with M02389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TCP1 alpha Antibody (M02389,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02389-tcp1-alpha-primary-antibodies-if-testing-5.jpgAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) IF analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). <br>
TCP1 alpha was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-TCP1 alpha Antibody (M02389) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02389-tcp1_alpha-primary-antibodies-wb-testing-1.pngAnti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)Western blot analysis of TCP1 using anti-TCP1 antibody (M02389). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1-6: human U2OS whole cell lysates, <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TCP1 antigen affinity purified monoclonal antibody (M02389) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibodyat a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd18-picoband-trade-antibody-m00458-1-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00458-1-ITGB2-primary-antibodies-IHC-testing-1_1.jpgAnti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3/2A10) IHC analysis of CD18 using anti-CD18 antibody (M00458-1). <br> CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00458-1-ITGB2-primary-antibodies-IHC-testing-2_1.jpgAnti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3/2A10) IHC analysis of CD18 using anti-CD18 antibody (M00458-1). <br> CD18 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00458-1-itgb2-primary-antibodies-wb-testing-3.jpgAnti-CD18 ITGB2 Antibody Picoband® (monoclonal, 1A3/2A10) Western blot analysis of CD18 using anti-CD18 antibody (M00458-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD18 antigen affinity purified monoclonal antibody (Catalog # M00458-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-catenin-picoband-trade-antibody-m00004-2-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-ctnnb1-primary-antibodies-wb-testing-1.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) Western blot analysis of beta Catenin using anti-beta Catenin antibody (M04085-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human A431 whole cell lysates,<br>
Lane 5: human RT4 whole cell lysates,<br>
Lane 6: human T-47D whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody (Catalog # M04085-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for beta Catenin at approximately 95 kDa. The expected band size for beta Catenin is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-2-beta-catenin-primary-antibodies-ihc-testing-2.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). <br> beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-2-beta-catenin-primary-antibodies-ihc-testing-3.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). <br> beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-2-beta-catenin-primary-antibodies-ihc-testing-4.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2).<br> beta Catenin was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-2-beta-catenin-primary-antibodies-fc-testing-5.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) Flow Cytometry analysis of SiHa cells using anti-beta Catenin antibody (M00004-2).<br> Overlay histogram showing SiHa cells stained with M00004-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-beta Catenin Antibody (M00004-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-2-beta-catenin-primary-antibodies-if-testing-6.jpgAnti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) IF analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). <br> beta Catenin was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cardiac-fabp-picoband-trade-antibody-m01734-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01734-Cardiac_FABP-primary-antibodies-WB-testing-1_1.jpgAnti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse heart tissue lysates, <br>Lane 2: mouse heart tissue lysates, <br>Lane 3: rat heart tissue lysates, <br>Lane 4: rat heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cardiac FABP antigen affinity purified monoclonal antibody (Catalog # M01734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01734-cardiac-fabp-primary-antibodies-ihc-testing-2.jpgAnti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (M01734). <br>Cardiac FABP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (M01734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01734-cardiac_fabp-primary-antibodies-elisa-testing-3.jpgAnti-Cardiac FABP Fabp3 Antibody Picoband® (monoclonal, 6C4) Sandwich ELISA - Recombinant rat Cardiac FABP protein standard curve.<br>
Use in combination with reagents from Rat Cardiac FABP ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1623).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smn1-2-picoband-trade-antibody-m03420-1-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03420-1-smn1-primary-antibodies-wb-testing-1.jpgAnti-SMN1/2 Antibody Picoband® (monoclonal, 2B10)Western blot analysis of SMN1/2 using anti-SMN1/2 antibody (M03420-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human A375 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat kidney tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMN1/2 antigen affinity purified monoclonal antibody (M03420-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1051) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMN1/2 at approximately 39 kDa. The expected band size for SMN1/2 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03420-1-smn1_2-primary-antibodies-ihc-testing-2.jpgAnti-SMN1/2 Antibody Picoband® (monoclonal, 2B10) IHC analysis of SMN1/2 using anti-SMN1/2 antibody (M03420-1). <br> SMN1/2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SMN1/2 Antibody (M03420-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03420-1-smn1_2-primary-antibodies-ihc-testing-3.jpgAnti-SMN1/2 Antibody Picoband® (monoclonal, 2B10) IHC analysis of SMN1/2 using anti-SMN1/2 antibody (M03420-1). <br> SMN1/2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SMN1/2 Antibody (M03420-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03420-1-smn1_2-primary-antibodies-ihc-testing-4.jpgAnti-SMN1/2 Antibody Picoband® (monoclonal, 2B10) IHC analysis of SMN1/2 using anti-SMN1/2 antibody (M03420-1).<br> SMN1/2 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-SMN1/2 Antibody (M03420-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ape1-picoband-trade-antibody-m00627-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00627-APE1-primary-antibodies-WB-testing-1_1.jpgAnti-APE1 APEX1 Antibody Picoband® (monoclonal, 5C11) Western blot analysis of APE1 using anti-APE1 antibody (M00627). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br>Lane 3: human COLO-320 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: Rabbit IgG, <br>Lane 6: Marker 1113, <br>Lane 7: human A549 whole cell lysates , <br>Lane 8: human PANC-1 whole cell lysates, <br>Lane 9: human 22RV1 whole cell lysates, <br>Lane 10: human MDA-MB-453 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APE1 antigen affinity purified monoclonal antibody (Catalog # M00627) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00627-APE1-primary-antibodies-IHC-testing-2_1.jpgAnti-APE1 APEX1 Antibody Picoband® (monoclonal, 5C11) IHC analysis of APE1 using anti-APE1 antibody (M00627). <br>APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-APE1 Antibody (M00627) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. "https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00627-apex1-primary-antibodies-fcm-testing-3.pngAnti-APE1 APEX1 Antibody Picoband® (monoclonal, 5C11) Flow Cytometry analysis of SiHa cells using anti-APE1 antibody (M00627). <br>
Overlay histogram showing SiHa cells stained with M00627 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APE1 Antibody (M00627, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stefin-b-picoband-trade-antibody-m02794-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02794-Stefin_B-primary-antibodies-WB-testing-1_1.jpgAnti-Stefin B CSTB Antibody Picoband® (monoclonal, 2B6) Western blot analysis of Stefin B using anti-Stefin B antibody (M02794). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysates, <br> Lane 2: human SW620 whole cell lysates, <br> Lane 3: human U-937 whole cell lysates, <br> Lane 4: human Caco-2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Stefin B antigen affinity purified monoclonal antibody (Catalog # M02794) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02794-Stefin_B-primary-antibodies-IHC-testing-2_1.jpgAnti-Stefin B CSTB Antibody Picoband® (monoclonal, 2B6) IHC analysis of Stefin B using anti-Stefin B antibody (M02794). <br> Stefin B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Stefin B Antibody (M02794) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02794-stefin_b-primary-antibodies-fcm-testing-3.jpgAnti-Stefin B CSTB Antibody Picoband® (monoclonal, 2B6) Flow Cytometry analysis of PC-3 cells using anti-Stefin B antibody (M02794).<br> Overlay histogram showing PC-3 cells stained with M02794 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Stefin B Antibody (M02794,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-19-picoband-trade-antibody-m02101-2-boster.html2026-03-24T05:18:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-2-Cytokeratin_19-primary-antibodies-WB-testing-1_1.jpgAnti-Cytokeratin 19 KRT19 Antibody Picoband® (monoclonal, 3D4) Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (M02101-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br> Lane 2: human placenta tissue lysates, <br>Lane 3: human SK-OV-3 whole cell lysates, <br>Lane 4: human COLO-320 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 19 antigen affinity purified monoclonal antibody (Catalog # M02101-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-2-Cytokeratin_19-primary-antibodies-IHC-testing-2_1.jpgAnti-Cytokeratin 19 KRT19 Antibody Picoband® (monoclonal, 3D4) IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (M02101-2). <br>Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-2-cytokeratin_19-primary-antibodies-if-testing-3.jpgAnti-Cytokeratin 19 KRT19 Antibody Picoband® (monoclonal, 3D4) IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (M02101-2).<br> Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-crm1-picoband-trade-antibody-m01180-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01180-crm1-primary-antibodies-wb-testing-1.jpgAnti-CRM1 XPO1 Antibody Picoband® (monoclonal, 5G3) Western blot analysis of CRM1 using anti-CRM1 antibody (M01180). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
Lane 4: mouse lung tissue lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human SMMC-7721 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates, <br>
Lane 8: human Jurkat whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CRM1 antigen affinity purified monoclonal antibody (Catalog # M01180) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01180-CRM1-primary-antibodies-IHC-testing-2_1.jpgAnti-CRM1 XPO1 Antibody Picoband® (monoclonal, 5G3) IHC analysis of CRM1 using anti-CRM1 antibody (M01180). <br>CRM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CRM1 Antibody (M01180) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01180-CRM1-primary-antibodies-IHC-testing-3_1.jpgAnti-CRM1 XPO1 Antibody Picoband® (monoclonal, 5G3) IHC analysis of CRM1 using anti-CRM1 antibody (M01180).<br> CRM1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CRM1 Antibody (M01180) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01180-4.pngAnti-CRM1 XPO1 Antibody Picoband® (monoclonal, 5G3) Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody (M01180). <br> Overlay histogram showing SiHa cells stained with M01180 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CRM1 Antibody (M01180,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01180-crm1-primary-antibodies-if-testing-5.jpgAnti-CRM1 XPO1 Antibody Picoband® (monoclonal, 5G3) IF analysis of CRM1 using anti-CRM1 antibody (M01180). <br>
CRM1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- CRM1 Antibody (M01180) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fhit-picoband-trade-antibody-m01200-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01200-FHIT-primary-antibodies-WB-testing-1_1.jpgAnti-FHIT Antibody Picoband® (monoclonal, 26H7) Western blot analysis of FHIT using anti-FHIT antibody (M01200). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human COLO-320 whole cell lysates, <br>Lane 2: human SW620 whole cell lysates, <br>Lane 3: human SGC-7901 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FHIT antigen affinity purified monoclonal antibody (Catalog # M01200) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01200-fhit-primary-antibodies-if-testing-2.jpgAnti-FHIT Antibody Picoband® (monoclonal, 26H7) IF analysis of FHIT using anti-FHIT antibody (M01200). <br>
FHIT was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-FHIT Antibody (M01200) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01200-fhit-primary-antibodies-fcm-testing-3.jpgAnti-FHIT Antibody Picoband® (monoclonal, 26H7) Flow Cytometry analysis of 293T cells using anti-FHIT antibody (M01200).<br>Overlay histogram showing 293T cells stained with M01200 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FHIT Antibody (M01200,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct3-picoband-trade-antibody-m05920-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05920-CCT3-primary-antibodies-WB-testing-1_1.jpgAnti-CCT3 Antibody Picoband® (monoclonal, 12H4) Western blot analysis of CCT3 using anti-CCT3 antibody (M05920). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human COLO-320 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CCT3 antigen affinity purified monoclonal antibody (Catalog # M05920) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05920-cct3-primary-antibodies-if-testing-2.jpgAnti-CCT3 Antibody Picoband® (monoclonal, 12H4) IF analysis of CCT3 using anti-CCT3 antibody (M05920). <br>
CCT3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-CCT3 Antibody (M05920) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-elafin-skalp-picoband-trade-antibody-m00364-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00364-Elafin-primary-antibodies-IHC-testing-1.jpgAnti-Elafin/Skalp PI3 Antibody (monoclonal, C7) IHC analysis of Elafin using anti-Elafin antibody (M00364). <br>Elafin was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Elafin Antibody (M00364) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00364-elafin-primary-antibodies-elisa-testing-2.jpgAnti-Elafin/Skalp PI3 Antibody (monoclonal, C7) Sandwich ELISA - Recombinant human Elafin/Skalp protein standard curve.<br>
Use in combination with reagents from Human Elafin/Skalp ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1117).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-interferon-gamma-picoband-trade-antibody-m00393-2-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00393-2-interferon-gamma-primary-antibodies-elisa-testing-1.jpgAnti-Interferon gamma IFNG Antibody (monoclonal, 8E9) Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.<br>
Use in combination with reagents from Rat Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0374).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pon1-picoband-trade-antibody-m00516-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00516-PON1-primary-antibodies-WB-testing-1.jpgAnti-PON1 Antibody Picoband® (monoclonal, 9D3) Western blot analysis of PON1 using anti-PON1 antibody (M00516). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse liver tissue lysates, <br>Lane 2: mouse liver tissue lysates, <br>Lane 3: rat liver tissue lysates,<br> Lane 4: rat liver tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PON1 antigen affinity purified monoclonal antibody (Catalog # M00516) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-pon1-primary-antibodies-elisa-testing-2.jpgAnti-PON1 Antibody Picoband® (monoclonal, 9D3) Sandwich ELISA - Recombinant mouse PON1 protein standard curve.<br>
Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1621).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pon1-picoband-trade-antibody-m00516-1-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-1-pon1-primary-antibodies-wb-testing-1_1_1.jpgAnti-PON1 Antibody Picoband® (monoclonal, 6B1) Western blot analysis of PON1 using anti-PON1 antibody (M00516-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: mouse liver tissue lysate,<br> Lane 2: mouse testis tissue lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PON1 antigen affinity purified monoclonal antibody (Catalog # M00516-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-1-pon1-primary-antibodies-elisa-testing-2.jpgAnti-PON1 Antibody Picoband® (monoclonal, 6B1) Sandwich ELISA - Recombinant mouse PON1 protein standard curve.<br>
Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1621).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pon1-picoband-trade-antibody-m00516-2-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-2-pon1-primary-antibodies-elisa-testing-1.jpgAnti-PON1 Antibody (monoclonal, 11H2) Sandwich ELISA - Recombinant mouse PON1 protein standard curve.<br>
Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1621).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-emerin-picoband-trade-antibody-m00714-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00714-Emerin-primary-antibodies-WB-testing-1_1.jpgAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10) Western blot analysis of Emerin using anti-Emerin antibody (M00714). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates, <br>Lane 2: human placenta tissue lysates, <br>Lane 3: human Caco-2 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates,<br>Lane 5: Rabbit IgG,<br> Lane 6: Marker 1113, <br>Lane 7: human Jurkat whole cell lysates.<br> Lane 8: human MDA-MB-453 whole cell lysates, <br>Lane 9: human SK-OV-3 whole cell lysates,<br> Lane 10: human SW620 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Emerin antigen affinity purified monoclonal antibody (Catalog # M00714) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig1_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)The expressions of ANKLE1, EMD, and LEMD2 and their relationship with clinical parameters of PRAD. A – C ANKLE1, EMD, and LEMD2 levels were increased in prostate cancer tissues compared to normal tissues (RNA-seq data from TCGA PRAD). The number of the normal group is 52, and the number of the tumor group is 499. D – G Higher ANKLE1 expression was associated with age, N stage, T stage, and M stage. H – K Higher EMD expression was associated with age, N stage, T stage, and M stage. L – O Higher LEMD2 expression was associated with age, N stage, T stage, and M stage. Compared with indicated group, * p < 0.05, ** p < 0.01, *** p < 0.001. n.s., no significant difference <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig2_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)The prognostic analysis of ANKLE1, EMD, and LEMD2 in prostate cancer. A – C The progress-free interval of ANKLE1, EMD, and LEMD2 mRNA level in prostate cancer patients (Kaplan-Meier plotter, tumor samples: n =499). D – F The overall survival of ANKLE1, EMD, and LEMD2 mRNA level in prostate cancer patients (Kaplan-Meier plotter, tumor samples: n = 499). G – L Time-dependent survival ROC curve analysis of ANKLE1, EMD, and LEMD2 to predict 3-, 5-, and 10-year survival rates <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig3_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)qPCR, WB, and IHC validation of ANKLE1, EMD, and LEMD2 in prostate cancer. A – C The mRNA levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens were validated by qPCR. D – I The protein levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens were validated by WB and IHC. The data (means ± SEM) shown ( A – C , n = 5; D – I , n = 3) were representative of three separate experiments. Compared with the indicated group, * p < 0.05, ** p < 0.01, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig6_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)Associations of ANKLE1, EMD, and LEMD2 expressions with immunomodulators and chemokines from the TISIDB database. A – I Correlations between immunomodulators (including immune inhibitors, immunostimulators, and MHC molecules) and the expression levels of ANKLE1, EMD, and LEMD2. J – L Correlations between chemokines and the expression levels of ANKLE1, EMD, and LEMD2 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig7_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)Comparison of KM survival curves of ANKLE1, EMD, and LEMD2 expressions based on immune cells. A High ANKLE1 levels enriched in resting NK cells had worse OS in PRAD. B High EMD levels enriched in resting NK cells had worse OS in PRAD. C High LEMD2 levels enriched in resting NK cells had worse OS in PRAD <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-12957_2022_2640_fig8_html.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10)The analysis of mutation, CNV, and methylation for ANKLE1, EMD, and LEMD2 expressions in PRAD. A – C Heat map showing the correlations between ANKLE1, EMD, and LEMD2 mRNA levels and somatic mutations, CNV, and methylation in prostate cancer through the UCSC Xena database <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02640-z'>35650630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-emerin-primary-antibodies-ihc-testing-2.jpgAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10) IHC analysis of Emerin using anti-Emerin antibody (M00714). <br>Emerin was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Emerin Antibody (M00714) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-emerin-primary-antibodies-ihc-testing-3.jpgAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10) IHC analysis of Emerin using anti-Emerin antibody (M00714). <br>Emerin was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Emerin Antibody (M00714) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-emerin-primary-antibodies-fc-testing-4.pngAnti-Emerin EMD Antibody Picoband® (monoclonal, 5A10) Flow Cytometry analysis of A431 cells using anti-Emerin antibody (M00714). <br> Overlay histogram showing A431 cells stained with M00714 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Emerin Antibody (M00714,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-p-glycoprotein-antibody-a00049-1-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00049-1-P_Glycoprotein-primary-antibodies-WB-testing-1_1.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human THP-1 whole cell lysates,<br>Lane 2: human A375 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein antigen affinity purified polyclonal antibody (Catalog # A00049-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein at approximately 160KD. The expected band size for P Glycoprotein is at 141KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-thnov06p0204g001.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®High Expression of P-gp Protein in Human or Rat Fibroblasts of Adhesion Peritoneum. (A and B) Representative immunohistochemical images for P-gp, a-SMA, vimentin, and collagen I in human (A) and rat (B) peritoneal adhesion tissues and adjacent normal peritoneum tissues. (C) Quantitative analysis of immunohistochemical results indicates that P-gp is highly expressed in the adhesion fibroblasts (AFB). (D) Western blot analysis shows that the P-gp protein expression was significantly upregulated in cultured human and rat AFB. ** P < 0.01. See also Figure .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4729769/&orig_host=www.ncbi.nlm.nih.gov'>26877779</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-thnov06p0204g004.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®Up-regulation of P-gp Expression Elevated I Cl,vol and RVD of Peritoneal Fibroblast by Phosphorylating Chloride Channel-3 (ClC-3) tyrosine 342 via Src Tyrosine Kinase. (A) Comparison of volume-activated chloride currents in AFB and NFB. Iso, isotonic bath solution; Hypo, 47% hypotonic bath solution; Hyper, 47% hypertonic bath solution. (B) Suppression of MDR1 expression by sh-MDR1 transfection on I Cl,vol in AFB. Cells successfully transfected with sh-MDR1 or sh-NC (green) were selected for whole-cell current recordings under the fluorescence microscope. (C and D) Differential capability of RVD in AFB and NFB and inhibition of RVD by silencing of MDR1 expression in AFB. (C) Changes in AFB and NFB cell volumes in isotonic (Iso) and 47% hypotonic (Hypo) bath solutions and RVD capacity in the hypotonic bath solution. (D) RVD capacity of AFB after treatment with Sh-NC or Sh-MDR1 (n = 20). (E) P-gp promoted phosphorylation of human ClC-3 tyrosine 342. (left panels) NIH-3T3 cells were co-transfected with human MDR1-GFP vectors and ClC-3-Flag or ClC-3-Y432F. Immunoblots of IP with anti-phosphotyrosine and anti-ClC-3 antibodies from extracts of NIH-3T3 cells were probed with an antibody against Flag. (right panels) Effects of down-regulation of P-gp expression or inhibition of Src kinase by SU6656 on phosphorylation of human ClC-3 tyrosine induced by TGF β1 and hypotension. pTyr: phosphotyrosine. **: P < 0.01. (F) P-gp-mediated Src phosphorylation induced by hypotonicity and TGF β1. Representative western blot (upper) and densitometric analysis (lower) showed that phospho-Src (pY 416 ) induced by hypotonicity and TGF β1 was abolished by down-regulation of P-gp expression. **: P < 0.01. (G) P-gp binds to Src kinase. (left two panels) P-gp does not bind ClC-3. Immunoblots of co-IP with anti-Flag antibody from extracts of NIH-3T3 cells co-transfected with MDR1/GFP and ClC-3/Flag, probed with antibodies against P-gp or ClC-3 (upper panel). Control experiment with anti-Flag antibody from extracts of NIH-3T3 cells co-transfected with K18/GFP and ClC-3/Flag probed with anti-K18 (centre panel). (Lower panel) Co-IP with anti-GFP antibody from NIH-3T3 cells extract was probed with anti-Src and anti-P-gp. Cells were transfected with human MDR1-GFP vectors.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4729769/&orig_host=www.ncbi.nlm.nih.gov'>26877779</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-thnov06p0204g003.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®TGF-β1 Up-regulated MDR1 Expression by TGF-β1/Smad Signaling Pathway and Histone H3 Acetylation. (A) The effect of physical factors, cytokines and growth factors associated with peritoneal injury on MDR1 mRNA expression in NFB. (B and C) Reinfusion of peritoneal fluid obtained from rats with peritoneal adhesions resulted in more serious peritoneal adhesions. (B) Peritoneal injury increased the concentration of TGF-β1 in peritoneal fluid, which reached the highest level at day 7. (C) Representative photographic images of rat peritoneal adhesions. Peritoneal fluid with high endogenous TGF-β1 was reinfused into peritoneal cavities of rats with mild peritoneal injuries, resulting in more serious peritoneal adhesions of higher grade and a greater rate of adhesions (For detailed statistical results, see ). (D and E) TGF-β1 promoted peritoneal adhesion formation and P-gp expression of adhesion tissue. (D) Representative photographic images of rat peritoneal adhesions. Intraperitoneal injection of exogenous TGF-β1 (150 ng/kg) induced more serious peritoneal adhesions of higher grade and a greater rate of adhesions (For detailed statistical results, see ). (E) Intraperitoneal injection of TGF-β1 upregulated P-gp expression in adhesion tissues. ** P < 0.01 vs Control. (F and G) Inhibition of the TGF-β1/Smad pathway prevented induction of P-gp expression by TGF-β1. (F) TGF-β type I receptor (TβRI) inhibitor SB431542 inhibited P-gp expression induced by TGF-β1. (G) Silencing of Smad 2 or 3 expression by transfection with Si-Smad 2 or -Smad 3, respectively, prevented induction of P-gp expression by TGF-β1. (H - K) TGF-β1 enhances activity of the MDR1 promoter via the TGF-β1/Smad signaling pathway and promotion of histone H3 acetylation. Chromatin immunoprecipitation (ChIP) analysis of the binding of Smad2/3 and histone H3 to the MDR1 promoter in AFB (H) and acetylated H3 at the MDR1 promoter in rat NFB and NIH3T3 cells treated with TGF-β1 (I). RPL30 was used as a positive control. (J) Detection of global acetylated histone H3 in rat NFB treated with different factors. (K) Induction of MDR1 promoter activity by co-transfection with either histone H3, Smad 2, or Smad 3 expression plasmids plus pMDR1(-1202) reporter plasmid and TGF-β1 or the histone deacetylase (HDAC) inhibitor panobinostat (LBH589). TGF-β type I receptor (TβRI) inhibitor SB431542 and histone acetyltransferase CBP/p300 inhibitor C646 abolished the increased activity induced by TGF-β1. * P < 0.05; ** P < 0.01. (L) Schematic model depicting the proposed mechanism of up-regulation of MDR1 expression induced by TGF-β1 via the TGF-β-Smad signaling pathway and histone H3 acetylation.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4729769/&orig_host=www.ncbi.nlm.nih.gov'>26877779</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-thnov06p0204g006.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®Altering P-gp Expression or Blocking I Cl,vol affects Cell Migration and Proliferation. (A and B) Migratory ability of human and rat AFB overexpressing P-gp and NFB. Confluent monolayers were scratched and then cultured in medium containing 0.5% FCS for 48 h (A). Data shown in (B) are mean ± SEM. (C) Representative photographs of wound-healing motility assays for rat AFB cells transiently transfected with siRNA against MDR1. (D) Average migratory width of AFB cells treated with MDR1 siRNA or inhibitors. Data are mean ± SEM. (E and F) Comparison of proliferative capability between rat AFB and NFB cells (E) and quantification of multiple visual fields (n = 4, F). Bright-field photographs of primary cultured live cells were taken at different times. Dark areas indicate peritoneal adhesion tissue or adjacent normal peritoneum tissue. (G) Growth curve for rat AFB cells transfected with si-MDR1 or treated with different inhibitors. Cells were cultured in 96-well plates in medium with 10% serum. Relative cell numbers were determined by CCK-8 colorimetric assay. Data shown are the results of three independent experiments (mean ± SEM). si-MDR1 transfection and all inhibitors except for tariquidar significantly inhibited the proliferation of AFB cells at day 5 and 7 ( P < 0.01 vs si-NC).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4729769/&orig_host=www.ncbi.nlm.nih.gov'>26877779</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-p_glycoprotein-primary-antibodies-if-testing-2.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® IF analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1). <br>
P Glycoprotein was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-P Glycoprotein Antibody (A00049-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-1-p_glycoprotein-primary-antibodies-fcm-testing-3.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-P Glycoprotein antibody (A00049-1).<br>Overlay histogram showing U20S cells stained with A00049-1 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein Antibody (A00049-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control."
https://www.bosterbio.com/products/primary-antibodies/anti-atf4-picoband-trade-antibody-a00371-1-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00371-1-atf4-primary-antibodies-wb-testing-1_1.jpgAnti-ATF4 Antibody Picoband® Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SW620 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00371-1-ATF4-primary-antibodies-IHC-testing-2_1.jpgAnti-ATF4 Antibody Picoband® IHC analysis of ATF4 using anti-ATF4 antibody (A00371-1).<br>ATF4 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ATF4 Antibody (A00371-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00371-1-ATF4-primary-antibodies-IHC-testing-3_1.jpgAnti-ATF4 Antibody Picoband® IHC analysis of ATF4 using anti-ATF4 antibody (A00371-1).<br>ATF4 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ATF4 Antibody (A00371-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-calcitonin-picoband-trade-antibody-a02352-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02352-Calcitonin-primary-antibodies-IHC-testing-1_1.jpgAnti-Calcitonin/Calca Antibody IHC analysis of Calcitonin using anti-Calcitonin antibody (A02352).<br>Calcitonin was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Calcitonin Antibody (A02352) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02352-Calcitonin-primary-antibodies-IHC-testing-2_1.jpgAnti-Calcitonin/Calca Antibody IHC analysis of Calcitonin using anti-Calcitonin antibody (A02352).<br>Calcitonin was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Calcitonin Antibody (A02352) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cd5-picoband-trade-antibody-a00480-2-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-2-cd5-primary-antibodies-wb-testing-1_1.jpgAnti-CD5 Antibody Picoband®Western blot analysis of CD5 using anti-CD5 antibody (A00480-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (A00480-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67 kDa. The expected band size for CD5 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-2-cd5-primary-antibodies-wb-testing-2.jpgAnti-CD5 Antibody Picoband®Western blot analysis of CD5 using anti-CD5 antibody (A00480-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates,<br>
Lane 2: mouse spleen tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (A00480-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67-70 kDa. The expected band size for CD5 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-2-cd5-primary-antibodies-ihc-testing-1.jpgAnti-CD5 Antibody Picoband®IHC analysis of CD5 using anti-CD5 antibody (A00480-2). <br>CD5 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD5 Antibody (A00480-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-2-cd5-primary-antibodies-fcm-testing-1.jpgAnti-CD5 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-CD5 antibody (A00480-2). <br>
Overlay histogram showing Jurkat cells stained with A00480-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD5 Antibody (A00480-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cdk6-picoband-trade-antibody-a00358-boster.html2026-03-24T05:18:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00358-cdk6-primary-antibodies-wb-testing-1_1.jpgAnti-Cdk6 Antibody Picoband® Western blot analysis of Cdk6 using anti-Cdk6 antibody (A00358). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk6 antigen affinity purified polyclonal antibody (Catalog # A00358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk6 at approximately 37 kDa. The expected band size for Cdk6 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00358-Cdk6-primary-antibodies-IHC-testing-2_1.jpgAnti-Cdk6 Antibody Picoband® IHC analysis of Cdk6 using anti-Cdk6 antibody (A00358).<br>Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (A00358) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00358-Cdk6-primary-antibodies-IHC-testing-3_1.jpgAnti-Cdk6 Antibody Picoband® IHC analysis of Cdk6 using anti-Cdk6 antibody (A00358).<br>Cdk6 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (A00358) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-flip-picoband-trade-antibody-a01295-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01295-1-FLIP-primary-antibodies-WB-testing-1_1.jpgAnti-FLIP/CFLAR Antibody Picoband® Western blot analysis of FLIP using anti-FLIP antibody (A01295-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates, <br>Lane 3: human COLO-320 whole cell lysates,<br>Lane 4: human PANC-1 whole cell lysates,<br>Lane 5: human HepG2 whole cell lysates,<br>Lane 6: human MDA-MB-231 whole cell lysates,<br>Lane 7: mouse NIH3T3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLIP antigen affinity purified polyclonal antibody (Catalog # A01295-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLIP at approximately 43KD. The expected band size for FLIP is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-1-cflar-primary-antibodies-ihc-testing-4.jpgAnti-FLIP/CFLAR Antibody Picoband®IHC analysis of FLIP/CFLAR using anti-FLIP/CFLAR antibody (A01295-1). <br>FLIP/CFLAR was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLIP/CFLAR Antibody (A01295-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01295-1-FLIP-primary-antibodies-IHC-testing-2_1.jpgAnti-FLIP/CFLAR Antibody Picoband® IHC analysis of FLIP using anti-FLIP antibody (A01295-1).<br>FLIP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FLIP Antibody (A01295-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01295-1-FLIP-primary-antibodies-IHC-testing-3_1.jpgAnti-FLIP/CFLAR Antibody Picoband® IHC analysis of FLIP using anti-FLIP antibody (A01295-1).<br>FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FLIP Antibody (A01295-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-collagen-ii-picoband-trade-antibody-a00517-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig2_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®Osteocyte-derived extracellular vesicles promote chondrocyte catabolism and inhibit anabolism in vitro and in vivo. A A schematic diagram illustrated that primary human osteoarthritic chondrocytes were treated with extracellular vesicles (EVs) extracted from low stress/high stress tissues (LS/HS-EVs). B The nanoparticle tracking analysis (NTA) analysis. C Representative confocal images showing the uptake of LS/HS-EVs in chondrocytes. Scale bar, 30 μm. D Alcian blue staining. Scale bar, 1 mm. E Western blot analysis of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression ( n = 3). F A schematic diagram illustrating the administration of intra-articular injection of LS/HS-EVs to destabilization of the medial meniscus (DMM) mice. G Representative confocal images of PKH26-labeled osteocyte-EVs in the cartilage. Scale bar, 50 μm. H The Safranin O images ( n = 8). Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. I A schematic diagram illustrated that primary mouse chondrocytes were treated with Static/Load-EVs. J The NTA analysis. K Representative confocal fluorescence micrographs showing the uptake of EVs in chondrocytes. Scale bar, 30 μm. L Alcian blue staining. Scale bar, 2 mm. M Western blot analysis of ColII, Acan, Sox9, Mmp13 and Adamts5 protein level ( n = 3). N A schematic diagram illustrating the administration of intra-articular injection of Static-EVs and Load-EVs to DMM mice. O Representative confocal images of PKH26-labeled MLO-Y4-EVs in the cartilage. Scale bar, 50 μm. P The Safranin O images of cartilage ( n = 8). Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Q A schematic diagram illustrating the strategy for reducing osteocyte-derived EVs in DMM mice. R Representative immunohistochemical staining of Cd63 in subchondral bone (SCB) osteocytes ( n = 8). Scale bars: 50 μm. S The Safranin O images ( n = 8). Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Data are presented as the mean ± SD. Data was analyzed by unpaired two-tailed Student’s t -test ( R ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig3_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®MiRNAs were responsible for osteocyte-derived extracellular vesicles in regulating cartilage metabolism. A A schematic diagram illustrating the co-culturing system. Scale bar, 30 μm. B miR-223 expression in primary mouse chondrocytes ( n = 3). C Images of primary mouse osteocytes isolated from wild-type (WT)- or osteocytes-specific Dicer knockout mice (cKO). Scale bar, 100 μm. D Western blot (left panel) and semi-quantitative analysis (right panel) of Dicer protein level in WT/cKO osteocytes ( n = 3). E A schematic diagram illustrating the coculture of primary mouse chondrocytes and extracellular vesicles (EVs) from WT/cKO osteocytes subjected to mechanical loading (WT-EVs / cKO-EVs). F Alcian blue staining. Scale bar, 2 mm. G qPCR analysis of the Col2a1 , Acan , Sox9 , Mmp13 and Adamts5 levels in chondrocytes treated with WT-EVs or cKO-EVs ( n = 3). H Western blot analysis (the left panel) and quantitative analysis (the right panel) of ColII, Acan, Sox9, Mmp13 and Adamts5 protein level in chondrocytes treated with WT-EVs or cKO-EVs ( n = 3). I A schematic diagram showed that WT/cKO mice were performed Sham/destabilization of the medial meniscus (DMM) surgery. J Western blot (left panel) and semi-quantitative analysis (right panel) of Dicer protein level in cKO mice ( n = 5). K Heat map of miRNA qPCR array ( n = 3). L Safranin O images ( n = 8). Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. M The Osteoarthritis Research Society International (OARSI) scores ( n = 8). N Representative confocal images of Acan, Sox9, Mmp13 and Adamts5 expression in cartilage from the indicated groups. Scale bars: 50 μm. O The quantitative analysis of Acan, Sox9, Mmp13 and Adamts5 protein ( n = 8). Data are presented as the mean ± SD. P values are from unpaired two-tailed Student’s t -test ( B , D , G , H and J ), Kruskal–Wallis test followed by two-tailed Mann–Whitney U test ( M ), one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test ( O ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig4_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®MiR-23b-3p was the key mediator of osteocyte-derived extracellular vesicles in regulating cartilage metabolism. A A schematic diagram illustrating the separation of extracellular vesicles (EVs) and their enrichment from human tissues and mouse plasma. Heatmaps of relative miRNA differences ( B ) and enrichment analysis ( C ). D The miR-23b-3p levels in LS-EVs and HS-EVs ( n = 5). E The miR-23b-3p levels in subchondral bone (SCB) osteocytes from WT and Dicer-cKO mice with destabilization of the medial meniscus (DMM) surgery ( n = 5). F The miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues ( n = 20). G The miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery ( n = 6). H The pre-miR-23b-3p levels in cartilage and SCB osteocytes from human LS/HS tissues ( n = 20). I The pre-miR-23b-3p levels in cartilage and SCB osteocytes from mice with DMM surgery ( n = 6). J Alcian blue staining. Scale bar in upper panel, 1 mm. Scale bar in lower panel, 2 mm. K Western blot analysis of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in primary human and mouse chondrocytes transfected with nc or mimic ( n = 3). L The schematic depicted the overexpression of miR-23b-3p in cartilage through intraarticular injection of rAAV5-miR-23b-3p-mcherry (rAAV5-miR-23b-3p) or negative control (rAAV5-nc) in 8-week-old male mice following DMM surgery ( n = 8). M The Safranin O images of cartilage. Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. Data are presented as the mean ± SD. Data was analyzed by unpaired two-tailed Student’s t -test ( D , E , G , I and K ) and paired two-tailed Student’s t -test ( F and H ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig5_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®Osteocyte-derived miR-23b-3p regulate cartilage metabolism during osteoarthritis progression. A A schematic diagram illustrating the use of extracellular vesicles (EVs) derived from MLO-Y4 cells transfected with either a negative control (nc mimic-EVs) or a miR-23b-3p mimic (mimic-EVs), as well as EVs obtained from loaded MLO-Y4 cells transfected with either a negative control (nc inhibitor-EVs) or a miR-23b-3p inhibitor (inhibitor-EVs), which were subsequently added to primary mouse chondrocytes. B Alcian blue staining. Scale bar, 2 mm. C Western blot analysis of ColII, Acan, Sox9, Mmp13 and Adamts5 protein expression in primary mouse chondrocytes ( n = 3). D qPCR analysis of the Col2a1 , Acan , Sox9 , Mmp13 and Adamts5 levels in primary mouse chondrocytes ( n = 3). E A schematic diagram illustrating mouse study in which the Dmp1 cre mice were administered tail vein injection with rAAV9DSS 6 -FLEx-miR-23b-3p (rAAV9DSS 6 -miR-23b-3p) or rAAV9DSS 6 -FLEx-nc (rAAV9DSS 6 -nc) in destabilization of the medial meniscus (DMM) or sham surgery mice, to specifically overexpress miR-23b-3p expression in osteocytes ( n = 7). F Safranin O images ( n = 7). Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. G The Osteoarthritis Research Society International (OARSI) scores ( n = 7). H A schematic diagram illustrating the mouse study in which Dmp1 cre mice were administered tail vein injection with rAAV9DSS 6 -FLEx-miR-23b-3p-RNAi (rAAV9DSS 6 -miR-23b-3p-RNAi) or rAAV9DSS 6 -FLEx-nc (rAAV9DSS 6 -nc) in DMM or sham surgery mice, to specifically antagonize miR-23b-3p expression in osteocytes ( n = 7). I Safranin O images. Scale bar in upper panel, 200 μm. Scale bar in lower panel, 100 μm. J The OARSI scores ( n = 7). Data are presented as the mean ± SD. P values are from unpaired two-tailed Student’s t-test ( D ), Kruskal–Wallis test followed by two-tailed Mann–Whitney U test ( G and J ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig6_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®MiR-23b-3p inhibits mitophagy by targeting Otud4 to skew the metabolism of chondrocytes. A Gene Ontology (GO) enrichment and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of potential targets for regulating cartilage metabolism from downregulated genes from human primary osteoarthritic chondrocytes transfected with negative control (nc) or miR-23b-3p mimics (mimic) using RNAseq. B Representative electron microscopy images of mitophagy in chondrocytes from low stress (LS) and high stress (HS) areas of clinical samples. Scale bars, 1 μm. C Mitophagy detection. Scale bars, 100 μm. D JC-1 staining. Scale bars, 50 μm. E Alcian blue staining. Scale bar, 1 mm. F Western blot analysis of PARKIN, PINK, MFN2, LC3B, DRP1, COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in human primary osteoarthritic chondrocytes ( n = 3). G Venn diagram of miR-23b-3p targets. H qPCR analysis of OTUD4 levels in human samples ( n = 12), Sham/destabilization of the medial meniscus (DMM) mice samples ( n = 6), chondrocytes with nc/mimic transfection ( n = 3) and chondrocytes with nc/inhibitor transfection ( n = 3). I The representative confocal images of OTUD4 of cartilage in human samples ( n = 10). Scale bars, 100 μm. J The representative confocal images of Otud4 of cartilage in Sham or DMM mice ( n = 6). Scale bars, 50 μm. K Dual luciferase reporter activities after transfecting 239 T cells with miR-23b-3p and reporter carrying 3’ UTR in the long form of OTUD4 ( n = 6). L Mitophagy detection. Scale bars, 100 μm. M JC-1 staining. Scale bars, 50 μm. N Alcian blue staining. Scale bar, 1 mm. O Western blot analysis of OTUD4, PARKIN, PINK, MFN2, LC3B, DRP1, COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in vehicle- and Lv-OTUD4-treated human primary osteoarthritic chondrocytes transfected with nc or mimic ( n = 3). Data are presented as the mean ± SD. Data analyzed by paired two-tailed Student’s t -test ( I ), unpaired two-tailed Student’s t -test ( H ), one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test ( J and K ) or Hypergeometric test ( A ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-col2a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen II/COL2A1 Antibody Picoband® Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat cartilage tissue lysates,<br>
Lane 2: mouse cartilage tissue lysates,<br>
Lane 3: mouse auricular cartilage lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-1-41467_2025_59861_fig1_html.pngAnti-Collagen II/COL2A1 Antibody Picoband®Extracellular vesicles facilitate crosstalk between osteocytes and chondrocytes in osteoarthritis progression. A A schematic diagram illustrating the workflow utilizing primary human osteoarthritic chondrocytes co-cultured with osteocytes located in high stress (HS) and low stress (LS) region of the subchondral bone (SCB). B Levels of IL-1β, IL-6 and TNF-α measured by ELISA assays ( n = 6). C The Alcian blue staining. Scale bar, 1 mm. D qPCR analysis of COL2A1, ACAN, SOX9, MMP13 and ADAMTS5 levels in primary human osteoarthritic chondrocytes co-cultured with LS/HS tissues ( n = 3). E Western blot of COLII, ACAN, SOX9, MMP13 and ADAMTS5 protein expression in primary human osteoarthritic chondrocytes co-cultured with LS/HS tissues ( n = 3). F RNA-sequencing and Gene Ontology (GO) enrichment analysis of the genes in LS/HS tissues. G A heat map (top) and its quantitation (bottom) indicating the expression of genes associated with extracellular vesicles (EVs) biogenesis within LS/HS tissues. H A schematic diagram summarizing the biology of EV biogenesis. I Representative electron microscopy images of intraluminal vesicles (ILVs) in LS/HS tissues. Scale bar, 500 nm. J Illustration of the animal experimental protocol for osteoarthritis (OA) modeling using osteocyte-specific ILVs/EVs reporter mice. K Safranin O staining. Scale bar, 200 μm. L The Osteoarthritis Research Society International (OARSI) scores ( n = 6). M Localization of Cd63-GFP (green) in SCB osteocytes. Scale bar, 50 μm. SCB Subchondral bone, BM Bone marrow. N The semi-quantitative analysis of Cd63-GFP + positive cells in SCB osteocytes ( n = 6). O Localization of Cd63-GFP (green) in articular cartilage. The white arrows indicated Cd63-GFP (green). Scale bar, 50 μm. P The semi-quantitative analysis of Cd63-GFP + positive cells in articular cartilage ( n = 6). Data are presented as the mean ± SD. P values are from paired two-tailed Student’s t -test ( B , D ), Kruskal–Wallis test followed by two-tailed Mann–Whitney U test ( L ), one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test ( N , P ) or Hypergeometric test ( F ). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59861-5'>40399261</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-c-reactive-protein-picoband-trade-antibody-a00249-2-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00249-2-c_reactive_protein-primary-antibodies-wb-testing-1.jpgAnti-C Reactive Protein/Crp Antibody Picoband® Western blot analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates, <br>Lane 2: rat lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C Reactive Protein antigen affinity purified polyclonal antibody (Catalog # A00249-2) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C Reactive Protein at approximately 28KD. The expected band size for C Reactive Protein is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00249-2-C_Reactive_Protein-primary-antibodies-IHC-testing-2_1.jpgAnti-C Reactive Protein/Crp Antibody Picoband® IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-2).<br>C Reactive Protein was detected in paraffin-embedded section of rat cerebellum tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-C Reactive Protein Antibody (A00249-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00249-2-C_Reactive_Protein-primary-antibodies-IHC-testing-3_1.jpgAnti-C Reactive Protein/Crp Antibody Picoband® IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-2).<br>C Reactive Protein was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-C Reactive Protein Antibody (A00249-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00249-2-C_Reactive_Protein-primary-antibodies-IHC-testing-4_1.jpgAnti-C Reactive Protein/Crp Antibody Picoband® IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody (A00249-2).<br>C Reactive Protein was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-C Reactive Protein Antibody (A00249-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cystatin-c-picoband-trade-antibody-a00961-2-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00961-2-cystatin_c-primary-antibodies-wb-testing-1.jpgAnti-Cystatin C/CST3 Antibody Picoband® Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human COLO-320 whole cell lysates,<br>Lane 2: human placenta tissue lysates, <br>Lane 3: human HepG2 whole cell lysates,<br>Lane 4: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-2) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00961-2-cst3-primary-antibodies-ihc-testing-3.jpgAnti-Cystatin C/CST3 Antibody Picoband®IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody (A00961-2). <br>Cystatin C/CST3 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cystatin C/CST3 Antibody (A00961-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00961-2-13195_2025_1714_fig6_html.pngAnti-Cystatin C/CST3 Antibody Picoband®PBM inhibits neuronal apoptosis in mice with AD. A-B Analysis of JC-1 flow cytometry results in the mouse brain tissue. C Immunohistochemical staining of mouse brains using an anti-Cytc antibody (scale bars 100 μm and 20 μm, respectively) with arrows indicating Cytc. D ELISA was used to detect Cytc in mouse brain tissue. E WB was used to detect Caspase3, Caspase9, and Cytc in the mouse brain tissue. F RT-PCR was used to detect Bcl-2, Bax, Caspase3, Caspase9, and Cytc in mouse brains. Experimental data are presented as the mean ± standard deviation. Compared with the CON group, * p < 0.05, ** p < 0.01, *** p < 0.001; Compared with the AD group, # p < 0.05, ## p < 0.01, ### p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13195-025-01714-w'>40188044</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00961-2-Cystatin_C-primary-antibodies-IHC-testing-2_1.jpgAnti-Cystatin C/CST3 Antibody Picoband® IHC analysis of Cystatin C using anti-Cystatin C antibody (A00961-2).<br>Cystatin C was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cystatin C Antibody (A00961-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cystatin-c-picoband-trade-antibody-a00961-3-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00961-3-Cystatin_C-primary-antibodies-WB-testing-1_1.jpgAnti-Cystatin C/Cst3 Antibody Picoband® Western blot analysis of Cystatin C using anti-Cystatin C antibody (A00961-3).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse brain tissue lysates, <br>Lane 2: mouse spleen tissue lysates, <br>Lane 3: mouse heart tissue lysates, <br>Lane 4: mouse lung tissue lysates, <br>Lane 5: mouse kidney tissue lysates, <br>Lane 6: mouse testis tissue lysates, <br>Lane 7: mouse HEPA1-6 whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin C antigen affinity purified polyclonal antibody (Catalog # A00961-3) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin C at approximately 16KD. The expected band size for Cystatin C is at 16KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cathepsin-e-picoband-trade-antibody-a04874-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04874-1-Cathepsin_E-primary-antibodies-WB-testing-1_1.jpgAnti-Cathepsin E/CTSE Antibody Picoband® Western blot analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat stomach tissue lysates, <br>Lane 2: mouse stomach tissue lysates, <br>Lane 3: mouse SP20 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin E antigen affinity purified polyclonal antibody (Catalog # A04874-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin E at approximately 48KD. The expected band size for Cathepsin E is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04874-1-Cathepsin_E-primary-antibodies-IHC-testing-2_1.jpgAnti-Cathepsin E/CTSE Antibody Picoband® IHC analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1).<br>Cathepsin E was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cathepsin E Antibody (A04874-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04874-1-Cathepsin_E-primary-antibodies-IHC-testing-3_1.jpgAnti-Cathepsin E/CTSE Antibody Picoband® IHC analysis of Cathepsin E using anti-Cathepsin E antibody (A04874-1).<br>Cathepsin E was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cathepsin E Antibody (A04874-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cyp17a1-picoband-trade-antibody-a00615-3-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00615-3-cyp17a1-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® Western blot analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates,<br>
Lane 2: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyp17a1 antigen affinity purified polyclonal antibody (Catalog # A00615-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyp17a1 at approximately 57 kDa. The expected band size for Cyp17a1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00615-3-Cyp17a1-primary-antibodies-IHC-testing-2_1.jpgAnti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3).<br>Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00615-3-Cyp17a1-primary-antibodies-IHC-testing-3_1.jpgAnti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3).<br>Cyp17a1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00615-3-cyp17a1-primary-antibodies-if-testing-4.jpgAnti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® IF analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3). <br>
Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00615-3-cyp17a1-primary-antibodies-if-testing-5.jpgAnti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband® IF analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3). <br>
Cyp17a1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-dermatopontin-picoband-trade-antibody-a02642-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02642-1-dermatopontin-primary-antibodies-wb-testing-1.jpgAnti-Dermatopontin/DPT Antibody Picoband® Western blot analysis of Dermatopontin using anti-Dermatopontin antibody (A02642-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates, <br>Lane 3: rat lung tissue lysates, <br>Lane 4: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dermatopontin antigen affinity purified polyclonal antibody (Catalog # A02642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dermatopontin at approximately 20KD. The expected band size for Dermatopontin is at 24KD.
https://www.bosterbio.com/products/primary-antibodies/anti-esm1-picoband-trade-antibody-a04169-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04169-1-esm1-primary-antibodies-wb-testing-1.jpgAnti-ESM1 Antibody Picoband® Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br>Lane 1: recombinant human ESM1 protein 1ng. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cardiac-fabp-picoband-trade-antibody-a01734-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01734-1-fabp3-primary-antibodies-wb-testing-1.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband® Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: rat heart tissue lysates,<br>
Lane 3: mouse heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody (Catalog # A01734-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15 kDa. The expected band size for Cardiac FABP is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01734-1-Cardiac_FABP-primary-antibodies-IHC-testing-2_1.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband® IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1).<br>Cardiac FABP was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (A01734-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01734-1-Cardiac_FABP-primary-antibodies-IHC-testing-3_1.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband® IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1).<br>Cardiac FABP was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (A01734-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01734-1-Cardiac_FABP-primary-antibodies-IHC-testing-4_1.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband® IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1).<br>Cardiac FABP was detected in paraffin-embedded section of mouse heart tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (A01734-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01734-1-Cardiac_FABP-primary-antibodies-IHC-testing-5_1.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband® IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1).<br>Cardiac FABP was detected in paraffin-embedded section of rat heart tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cardiac FABP Antibody (A01734-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-fgf9-picoband-trade-antibody-a02274-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02274-fgf9-primary-antibodies-wb-testing-1.jpgAnti-FGF9 Antibody Picoband® Western blot analysis of FGF9 using anti-FGF9 antibody (A02274). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human COLO-320 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF9 antigen affinity purified polyclonal antibody (Catalog # A02274) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF9 at approximately 26KD. The expected band size for FGF9 is at 23KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fndc5-picoband-trade-antibody-a02538-1-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02538-1-FNDC5-primary-antibodies-WB-testing-1_1.jpgAnti-FNDC5 Antibody Picoband® Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat heart tissue lysates, <br>Lane 2: mouse heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hsp105-picoband-trade-antibody-a04168-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-wb-testing-1_1.jpgAnti-Hsp105/HSPH1 Antibody Picoband® Western blot analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp105 antigen affinity purified polyclonal antibody (Catalog # A04168) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp105 at approximately 105 kDa. The expected band size for Hsp105 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-wb-testing-2.jpgAnti-Hsp105/HSPH1 Antibody Picoband® Western blot analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp105 antigen affinity purified polyclonal antibody (Catalog # A04168) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Hsp105 at approximately 105 kDa. The expected band size for Hsp105 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-ihc-testing-3.jpgAnti-Hsp105/HSPH1 Antibody Picoband® IHC analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br> Hsp105 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-ihc-testing-4.jpgAnti-Hsp105/HSPH1 Antibody Picoband® IHC analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br> Hsp105 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-ihc-testing-5.jpgAnti-Hsp105/HSPH1 Antibody Picoband® IHC analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br> Hsp105 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-if-testing-6.jpgAnti-Hsp105/HSPH1 Antibody Picoband® IF analysis of Hsp105 using anti-Hsp105 antibody (A04168). <br> Hsp105 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04168-hsp105-primary-antibodies-fc-testing-7.pngAnti-Hsp105/HSPH1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Hsp105 antibody (A04168). <br> Overlay histogram showing HepG2 cells stained with A04168 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp105 Antibody (A04168,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-igfbp2-picoband-trade-antibody-a01373-2-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-WB-testing-1_1.jpgAnti-IGFBP2 Antibody Picoband® Western blot analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates, <br>Lane 2: mouse Neuro-2a whole cell lysates,<br>Lane 3: mouse HEPA1-6 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody (Catalog # A01373-2) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 40KD. The expected band size for IGFBP2 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-2_1.jpgAnti-IGFBP2 Antibody Picoband® IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).<br>IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-3_1.jpgAnti-IGFBP2 Antibody Picoband® IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).<br>IGFBP2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-4_1.jpgAnti-IGFBP2 Antibody Picoband® IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).<br>IGFBP2 was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-5_1.jpgAnti-IGFBP2 Antibody Picoband® IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).<br>IGFBP2 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01373-2-IGFBP2-primary-antibodies-IHC-testing-6_1.jpgAnti-IGFBP2 Antibody Picoband® IHC analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2).<br>IGFBP2 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01373-2-igfbp2-primary-antibodies-ihc-testing-7.jpgAnti-IGFBP2 Antibody Picoband® IF analysis of IGFBP2 using anti-IGFBP2 antibody (A01373-2). <br>
IGFBP2 was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IGFBP2 Antibody (A01373-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01373-2-igfbp2-primary-antibodies-fcm-testing-8.jpgAnti-IGFBP2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-IGFBP2 antibody (A01373-2).<br>
Overlay histogram showing RH35 cells stained with A01373-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGFBP2 Antibody (A01373-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-il36-alpha-picoband-trade-antibody-a09802-3-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09802-3-IL36_alpha-primary-antibodies-WB-testing-1_1.jpgAnti-IL36 alpha/IL36A Antibody Picoband® Western blot analysis of IL36 alpha using anti-IL36 alpha antibody (A09802-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat lung tissue lysates, <br>Lane 3: mouse brain tissue lysates, <br>Lane 4: mouse lung tissue lysates, <br>Lane 5: human Hela whole cell lysates,<br>Lane 6: human placenta tissue lysates, <br>Lane 7: human MCF-7 whole cell lysates,<br>Lane 8: human HepG2 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL36 alpha antigen affinity purified polyclonal antibody (Catalog # A09802-3) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL36 alpha at approximately 25KD. The expected band size for IL36 alpha is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09802-3-IL36_alpha-primary-antibodies-IHC-testing-2_1.jpgAnti-IL36 alpha/IL36A Antibody Picoband® IHC analysis of IL36 alpha using anti-IL36 alpha antibody (A09802-3).<br>IL36 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL36 alpha Antibody (A09802-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-klf4-picoband-trade-antibody-a00120-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00120-KLF4-primary-antibodies-WB-testing-1_1.jpgAnti-KLF4 Antibody Picoband® Western blot analysis of KLF4 using anti-KLF4 antibody (A00120). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human COLO-320 whole cell lysates,<br>Lane 3: rat stomach tissue lysates, <br>Lane 4: rat testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60KD. The expected band size for KLF4 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-ijcep0012-4253-f4.jpgAnti-KLF4 Antibody Picoband®miR-92 regulates KLF4 expression via binding to KLF43’-UTR in vitro . (A) Schematic representation of KLF43’-UTR as a target for miR-92. (B) WT or Mut miR-92 target sequences of the KLF43’-UTR. (C) Luciferase activity of cells co-transfected with the WT or Mut KLF4 3’-UTR reporter genes or negative control miR mimics (pMIR-reporter). **P<0.01 vs. NC and Mut-KLF. One-way ANOVA test with Bonferroni as a post hoc test was used in (C). miR, microRNA; KLF4, Kruppel like factor 4; 3’-UTR, 3-untranslated region; WT, wild type; Mut, mutant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6949870/&orig_host=www.ncbi.nlm.nih.gov'>31933825</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-ijcep0012-4253-f5.jpgAnti-KLF4 Antibody Picoband®Effects of miR-92 on KLF4 expression. (A) miR-92 inhibited KLF4 expression at the protein level. (B) miR-92 inhibited KLF4 expression at the mRNA level. *P<0.05 vs. NC. Student’s T-test was used in (B). miR, microRNA; KLF4, Kruppel like factor 4.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6949870/&orig_host=www.ncbi.nlm.nih.gov'>31933825</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00120-KLF4-primary-antibodies-IHC-testing-2_1.jpgAnti-KLF4 Antibody Picoband® IHC analysis of KLF4 using anti-KLF4 antibody (A00120).<br>KLF4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KLF4 Antibody (A00120) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00120-KLF4-primary-antibodies-IHC-testing-3_1.jpgAnti-KLF4 Antibody Picoband® IHC analysis of KLF4 using anti-KLF4 antibody (A00120).<br>KLF4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KLF4 Antibody (A00120) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-leptin-picoband-trade-antibody-a00479-4-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00479-4-Leptin-primary-antibodies-WB-testing-1_1.jpgAnti-Leptin Antibody Picoband® Western blot analysis of Leptin using anti-Leptin antibody (A00479-4). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse small intestine tissue lysates, <br>Lane 2: mouse kidney tissue lysates, <br>Lane 3: mouse Neuro-2a whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # A00479-4) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 23KD. The expected band size for Leptin is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00479-4-Leptin-primary-antibodies-IHC-testing-2_1.jpgAnti-Leptin Antibody Picoband® IHC analysis of Leptin using anti-Leptin antibody (A00479-4).<br>Leptin was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00479-4-Leptin-primary-antibodies-IHC-testing-3_1.jpgAnti-Leptin Antibody Picoband® IHC analysis of Leptin using anti-Leptin antibody (A00479-4).<br>Leptin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00479-4-Leptin-primary-antibodies-IHC-testing-4_1.jpgAnti-Leptin Antibody Picoband® IHC analysis of Leptin using anti-Leptin antibody (A00479-4).<br>Leptin was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-gastric-mucin-antibody-a02143-boster.html2026-03-24T05:18:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02143-gastric_mucin-primary-antibodies-wb-testing-1.jpgAnti-Gastric Mucin/MUC6 Antibody Picoband® Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse stomach tissue lysates, <br>Lane 2: human SGC-7901 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02143-gastric_mucin-primary-antibodies-wb-testing-2.pngAnti-Gastric Mucin/MUC6 Antibody Picoband®Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: Normal group-rat colon tissue lysates, <br>
Lane 2: Model group-rat colon tissue lysates, <br>
Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br>
Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br>
Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates, <br>
Lane 6: Western medicine treatment-rat colon tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 1:1000 overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cobra1-picoband-trade-antibody-a12025-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12025-1-cobra1-primary-antibodies-wb-testing-1.jpgAnti-COBRA1/NELFB Antibody Picoband® Western blot analysis of COBRA1 using anti-COBRA1 antibody (A12025-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat kidney tissue lysates, <br>Lane 2: mouse kidney tissue lysates, <br>Lane 3: human MDA-MB-231 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody (Catalog # A12025-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
https://www.bosterbio.com/products/primary-antibodies/anti-anp-picoband-trade-antibody-a01318-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01318-anp-primary-antibodies-wb-testing-1.jpgAnti-ANP/Nppa Antibody Picoband® Western blot analysis of ANP using anti-ANP antibody (A01318). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse heart tissue lysates, <br>Lane 2: mouse heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP antigen affinity purified polyclonal antibody (Catalog # A01318) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANP at approximately 17KD. The expected band size for ANP is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-ANP-primary-antibodies-IHC-testing-2_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318).<br>ANP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-ANP-primary-antibodies-IHC-testing-3_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318).<br>ANP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-ANP-primary-antibodies-IHC-testing-4_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318).<br>ANP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-anp-picoband-trade-antibody-a01318-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01318-1-anp-primary-antibodies-wb-testing-1_1.jpgAnti-ANP/Nppa Antibody Picoband® Western blot analysis of ANP using anti-ANP antibody (A01318-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat heart tissue lysates,<br>
Lane 2: mouse heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP antigen affinity purified polyclonal antibody (Catalog # A01318-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANP at approximately 19 kDa. The expected band size for ANP is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-1-ANP-primary-antibodies-IHC-testing-2_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318-1).<br>ANP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-1-ANP-primary-antibodies-IHC-testing-3_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318-1).<br>ANP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01318-1-ANP-primary-antibodies-IHC-testing-4_1.jpgAnti-ANP/Nppa Antibody Picoband® IHC analysis of ANP using anti-ANP antibody (A01318-1).<br>ANP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-pdcd10-antibody-a01879-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01879-1-pdcd10-primary-antibodies-wb-testing-1.jpgAnti-PDCD10 Antibody Picoband® Western blot analysis of PDCD10 using anti-PDCD10 antibody (A01879-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat smooth muscle tissue lysates, <br>Lane 2: mouse smooth muscle tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD10 antigen affinity purified polyclonal antibody (Catalog # A01879-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD10 at approximately 29KD. The expected band size for PDCD10 is at 25KD.
https://www.bosterbio.com/products/primary-antibodies/anti-pdgfrb-picoband-trade-antibody-a00096-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00096-1-pdgfrb-primary-antibodies-wb-testing-1_2.jpgAnti-PDGF Receptor beta/PDGFRB Antibody Picoband®Western blot analysis of PDGFRB using anti-PDGFRB antibody (A00096-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human U2OS whole cell lysates,<br>
Lane 3: rat ovary tissue lysates,<br>
Lane 4: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified polyclonal antibody (Catalog # A00096-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 190 kDa. The expected band size for PDGFRB is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00096-1-pdgfrb-primary-antibodies-fcm-testing-1.jpgAnti-PDGF Receptor beta/PDGFRB Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PDGFRB antibody (A00096-1). <br>
Overlay histogram showing SH-SY5Y cells stained with A00096-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDGFRB Antibody (A00096-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nf-kb-p65-picoband-trade-antibody-a00284-1-boster.html2026-03-28T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-wb-testing-1.jpgAnti-NF-kB p65/RELA Antibody Picoband® Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Raji whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: rat RH35 whole cell lysates,<br>
Lane 7: mouse RAW264.7 whole cell lysates,<br>
Lane 8: mouse HEPA1/6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 70 kDa. The expected band size for NF-kB p65 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig9_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 promotes tubular cell injury and AKI partially through ferroptosis. A HKC-8 was transfected with Ctrl-shR or CD44-shR plasmid and then treated with 5 μM erastin for 24 h. Representative micrographs show the Fe 2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. B and C Quantitative results of QPCR showing relative ( B ) GPX4 and ( C ) ACSL4 mRNA levels among different groups. ** P < 0.01 versus Ctrl-shR group; # P < 0.05 versus erastin with ctrl-shR ( n = 3). D HKC-8 was transfected with pcDNA3 or pHA-CD44 overexpression plasmid and then treated with 5 μM erastin for 24 h. Representative micrographs show the Fe 2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. E and F Quantitative result of QPCR showing relative ( E ) GPX4 and ( F ) ACSL4 mRNA levels among different groups. *** P < 0.001 versus pcDNA3 group; # P < 0.05 versus erastin with pcDNA3 ( n = 3). G Representative micrographs show the Fe 2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. H and I Quantitative result of QPCR showing relative H GPX4 and I ACSL4 mRNA level among different groups. *** P < 0.001 versus Ctrl-shR group; ## P < 0.01, ### P < 0.001 versus H/R with ctrl-shR ( n = 3). J Quantitative result showing MDA content among different groups. *** P < 0.001 versus Ctrl-shR group; # P < 0.05 versus H/R with ctrl-shR ( n = 3). K and L Quantitative result of QPCR showing relative ( K ) GPX4 and ( L ) ACSL4 mRNA levels among different groups. *** P < 0.001 versus pcDNA3 group; # P < 0.05 versus H/R with pcDNA3 ( n = 3). M Quantitative result showing MDA content among different groups. *** P < 0.001 versus pcDNA3 group; # P < 0.05 versus H/R with pcDNA3 ( n = 3). N Representative micrographs show the Fe 2+ content via FerroOrange staining in different groups, as indicated. Scale bar, 25 μm. O Quantitative result showing total iron content in kidney tissue among different groups. *** P < 0.001 versus wild-type mice upon sham group; ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). P and Q Quantitative result of QPCR showing relative P GPX4 and Q ACSL4 mRNA levels among different groups. ** P < 0.01, *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05, ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). R Quantitative result showing MDA content in kidney tissue among different groups. ** P < 0.01 versus wild-type mice upon sham group; # P < 0.05 versus wild-type mice upon IRI group ( n = 5). S Quantitative result showing total iron content in kidney tissue among different groups. *** P < 0.001 versus sham group; ## P < 0.01 versus IRI group injected with pcDNA3 ( n = 5). T and U Quantitative result of QPCR showing relative S GPX4 and T ACSL4 mRNA level among different groups. *** P < 0.001 versus sham group; # P < 0.05 versus IRI group injected with pcDNA3 ( n = 5). V Quantitative result showing MDA content in kidney tissue among different groups. *** P < 0.001 versus sham group; ### P < 0.001 versus IRI group injected with pcDNA3 ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fnut-12-1562509-g004.jpgAnti-NF-kB p65/RELA Antibody Picoband®Effects of n-6 PUFA on liver lipid peroxidation and the inflammatory marker NF-κB in rats with NASH induced by a choline-deficient diet. (A) Liver MDA levels, (B) PPAR-α mRNA expression in the liver. Data are expressed as mean ±SEM; n = 6/group. (C) NF-κB protein expression (~65 kDa) in the liver as analyzed by Western blotting, normalized to GAPDH, with a representative blot (left) and quantification (right). Protein molecular weight standards (kDa) are labeled on the left of each blot. Data are expressed as mean ± SEM; n = 6/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1562509/full'>40626231</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fnut-12-1562509-g005.jpgAnti-NF-kB p65/RELA Antibody Picoband®Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1562509/full'>40626231</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-feb413914-fig-0009-m.jpgAnti-NF-kB p65/RELA Antibody Picoband®Effects of OPN and OVE on activation of NF-ĸB pathways in LPS-stimulated RAW264.7 cells. Cells were pretreated with OVE or OPN at different concentrations for 1 h. Expression levels of p-NF-ĸB p65, NF-ĸB p65, p-IĸBα, and IĸBα were detected after 24 h of LPS treatment. (A) OPN treatment. (B) OVE treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ****P < 0.0001, compared to the LPS control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/2211-5463.13914'>39455284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fphar-08-00044-g006.jpgAnti-NF-kB p65/RELA Antibody Picoband®Celastrol inhibited inducible nitric oxide synthase (iNOS) expression and activation of NF-κB in optic nerve in EAE rats. (A) IHC staining of iNOS in optic nerve. (B) Quantification of iNOS-positive areas. (C) Quantitative real-time PCR analysis of iNOS expression. (D) Western blot analysis of iNOS expression. (E–G) Western blot analysis of IκBα, p65 and p-p65 expression, respectively. Treatment of celastrol reduced expression of iNOS and inhibited the activation of NF-κB in optic nerve in EAE rats. Scale bar: 100 μm. Data were shown as mean ± SD, n = 5. ∗∗ P < 0.01 versus control group, ## P < 0.01 versus EAE group, †† P < 0.01 versus low dosage of celastrol group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00044/full'>28239352</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fphar-08-00044-g004.jpgAnti-NF-kB p65/RELA Antibody Picoband®Celastrol regulated production of cytokines and activation of NF-κB in spinal cords of EAE rats. Celastrol dose-dependently downregulated the mRNA expression of INF-γ (A) and IL-17 (B) but upregulated IL-4 (C) in spinal cord of EAE rats. Data were shown as mean ± SD, n = 5. ∗∗ P < 0.01 versus control group, ## P < 0.01 versus EAE group, †† P < 0.01 versus low dosage of celastrol group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00044/full'>28239352</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig8_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 induces tubular cell injury through MAPK-NF-κB p65-silenced PGC-1α signaling. A and B HKC-8 was transfected with pcDNA3 or p-Flag-p65 overexpression plasmid for 24 h. Representative western blot ( A ) and graphical presentations of PGC-1α, CPT1a, BCL2, and cleaved caspase 3 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus pcDNA3 groups ( n = 3). C Quantitative PCR result showing relative mRNA level of PGC-1α. * P < 0.05 versus pcDNA3 groups ( n = 3). D Representative ChIP assay results showing the binding of p65 to PGC-1α gene promoter region. HKC-8 cells were transfected with pcDNA3 or p-Flag-p65 for 24 h. Cell lysates were precipitated with an antibody against p65, histone H3, or nonimmune IgG, and ChIP assay was performed for PGC-1α gene promoters. Total diluted lysate was used as total genomic input DNA. E–I HKC-8 was pre-treated with SB203580, PD98059 or PDTC at 1 h before transfection with pcDNA3 or p-HA-CD44 plasmid for 24 h. Representative western blot ( E ) and graphical presentations of F p-p38/p38, G p-ERK1/2/ERK1/2, H p-p65, and I PGC-1α protein expression levels are shown. ** P < 0.01, *** P < 0.001 versus pcDNA3 group; # P < 0.05, ## P < 0.01 versus pHA-CD44 group; †† P < 0.01, ††† P < 0.001 versus pHA-CD44 group; φφφ P < 0.001 versus pHA-CD44 group ( n = 3). J The heatmap exhibiting differentiated lipids of lipidomics sequencing between H/R with Crtl-shR group and H/R with CD44-shR group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig7_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 aggravates mitochondrial and FAO dysfunction and drives cell apoptosis through MAPK and NF-κB p65 signaling in vitro. A and B HKC-8 was transfected with Ctrl-shR or CD44-shR and then were incubated in basal culture medium in a 1% O 2 environment for 24 h and then were reoxygenated in normal O 2 for 6 h. Representative western blot ( A ) and graphical presentations of p-p38/p38, p-ERK1/2/ERK1/2, and p-p65 protein expression levels are shown. ** P < 0.01 versus Crtl-shR group; ## P < 0.01, ### P < 0.001 versus H/R with Crtl-shR group ( n = 3). C Representative micrographs show the expression of p-p65 and MitoSox staining in different groups, as indicated. Arrows indicate positive staining. Cells cultured on coverslips were stained with an antibody against p-p65 or were stained with MitoSox. Scale bar, 25 or 50 μm. D Graphic presentation shows the relative mRNA levels of PGC-1α in different groups as indicated. ** P < 0.01 versus Crtl-shR group; ## P < 0.01 versus H/R with Crtl-shR group ( n = 3). E and F Representative western blot ( E ) and graphical representations of PGC-1α, TOMM20, CPT1a, and PPARα protein expression levels are shown. * P < 0.05, ** P < 0.01 versus Crtl-shR group; # P < 0.05, ## P < 0.01 versus H/R with Crtl-shR group ( n = 3). G Representative micrographs show Nile Red staining and TUNEL assay in different groups, as indicated. Arrows indicate positive staining. Cells cultured on coverships were stained with Nile Red or TUNEL assay. Scale bar, 25 or 50 μm. H Co-localization of CD44 and cleaved caspase 3 in HKC-8 after H/R treatment. Cells cultured on coverships were subjected to immunostaining of CD44 (red) and cleaved caspase 3 (green). Scale bar, 50 μm. I and J Representative western blot ( I ) and graphical representations of BCL2, BAX, and cleaved caspase 3 protein expression levels are shown. ** P < 0.01 versus Crtl-shR group; # P < 0.05 versus H/R with Crtl-shR group ( n = 3). K and L HKC-8 was transfected with pcDNA3 or p-HA-CD44 overexpression plasmid and then were incubated in basal culture medium in a 1% O 2 environment for 24 h and then were reoxygenated in normal O 2 for 6 h. Representative western blot ( K ) and graphical representations of CD44, p-ERK1/2/ERK1/2, p-p38/p38 and p-p65 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus pcDNA3 group; # P < 0.05, ## P < 0.01 versus H/R with pcDNA3 group ( n = 3). M and N Representative western blot ( M ) and graphical presentations of PGC-1α, CPT1a, and PPARα protein expression levels are shown. * P < 0.05, *** P < 0.001 versus pcDNA3 group; # P < 0.05 versus H/R with pcDNA3 group ( n = 3). O Representative micrographs show MitoSox staining in different groups, as indicated. Arrow indicates positive staining. Cells cultured on coverships were stained with MitoSox. Scale bar, 25 μm. P and Q Representative western blot ( P ) and graphical representations of BCL2, BAX, and cleaved caspase 3 protein expression levels are shown. * P < 0.05, ** P < 0.01 versus pcDNA3 group; # P < 0.05; ## P < 0.01 versus H/R with pcDNA3 group ( n = 3). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-wb-testing-2.jpgAnti-NF-kB p65/RELA Antibody Picoband® Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HAP1-WT whole cell lysates,<br>
Lane 2: human HAP1-NF-kB p65 KO whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human Raji whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 70 kDa. The expected band size for NF-kB p65 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig6_html.pngAnti-NF-kB p65/RELA Antibody Picoband®Ectopic expression of CD44 impairs mitochondrial function and FAO through activating MAPK and NF-κB p65 signaling. A Representative micrographs show the expression of p-ERK1/2 and p-p38 in different groups, as indicated. Paraffin sections were stained with antibodies against p-ERK1/2 and p-p38. Arrows indicate positive staining. Scale bar, 50 μm. B and C Representative western blot ( B ) and graphical presentations of p-ERK1/2/ERK1/2 and p-p38/p38 protein levels are shown. ** P < 0.01, *** P < 0.001 versus sham group; ## P < 0.01, ### P < 0.001 versus IRI group injected with pcDNA3 ( n = 5). D Co-localization of CD44 and p65 in CD44 overexpression mice upon IRI. Frozen renal sections were subjected to immunostaining of CD44 (red) and p65 (green). Scale bar, 50 μm. E and F Representative western blot ( E ) and graphical presentations of p-p65, p65, and p-p65/p65 protein levels are shown. ** P < 0.01, *** P < 0.001 versus sham group; ## P < 0.01, ### P < 0.001 versus IRI group injected with pcDNA3 ( n = 5). G Co-localization of CD44 and PGC-1α in CD44 overexpression mice upon IRI. Frozen renal sections were subjected to immunostaining of CD44 (red) and PGC-1α (green). Scale bar, 50 μm. H and I Representative western blot ( H ) and graphical presentations of PGC-1α, TOMM20, PPARα, and CPT1a protein expression levels are shown. ** P < 0.01, *** P < 0.001 versus sham group; # P < 0.05, ## P < 0.01 versus IRI group injected with pcDNA3 ( n = 5). J Representative micrographs show the expression of perilipin 2, and LDs via Oil Red O staining in different groups, as indicated. Frozen kidney sections were subjected to Oil Red O staining or stained with an antibody against perilipin 2. Arrows indicate positive staining. Scale bar, 50 or 100 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig3_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 knockout ameliorates mitochondrial dysfunction and FAO deficiency in IRI mice. A GSEA shows that positive regulation of mitochondrial function and FAO was enriched in CD44 knockout mice versus wild-type mice upon IRI. NES, normalized enrichment score; FDR q -value < 0.25. B Graphic presentation shows the relative levels of renal expression of PGC-1α mRNA in different groups as indicated. *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05 versus wild-type mice upon IRI group ( n = 5). C Graphic presentation shows the relative levels of ATP production in 2 groups as indicated. ** P < 0.01 versus wild-type upon IRI group ( n = 5). D and E Representative western blot ( D ) and graphical presentations of PGC-1α and TOMM20 protein expression levels are shown. ** P < 0.01, *** P < 0.001 versus wild-type mice upon sham group; ## P < 0.01, ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). F Co-localization of CD44 and PGC-1α in CD44 gene ablation mice upon IRI. Frozen kidney sections were subjected to immunostaining of CD44 (red) and PGC-1α (green). Scale bar, 50 μm. G Representative micrographs show the expression of PGC-1α, TOMM20, mitochondrial morphology via TEM, and mitochondrial ROS via MitoSox staining in different groups, as indicated. Arrows indicate positive staining or impaired mitochondria. Scale bar, 50 or 1 μm, as indicated. H Graphic presentation shows the relative mRNA levels of renal expression of PPARα, CPT1, CPT2, and ACOX1 mRNA in different groups as indicated. ** P < 0.01, *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05, ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). I Representative micrographs show the abundance of LDs via TEM in 2 groups, as indicated. Arrows indicate LDs. Scale bar, 1 μm. J and K Representative western blot ( J ) and graphical presentations of CPT1a and PPARα protein expression levels are shown. * P < 0.05, ** P < 0.01 versus wild-type mice upon sham group; # P < 0.05, ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). L Representative micrographs show the expression of PPARα, CPT1a, perilipin 2, and LDs via Oil Red O staining in different groups, as indicated. Arrows indicate positive staining. Frozen kidney sections were subjected to Oil Red O staining or stained with antibodies against PPARα, CPT1a, and perilipin 2. Scale bar, 50 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig4_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 promotes AKI progression through inducing MAPK and NF-κB p65 signaling. A Representative heatmap gene expression of RNA sequencing analysis shows that CD44 is involved with MAPK and NF-κB signaling pathway. B and C GSEA shows that negative regulation of MAPK and NF-κB pathway was enriched in CD44 knockout mice versus wild-type mice upon IRI. NES, normalized enrichment score; FDR q -value < 0.25. D Representative micrographs show the expression of p-ERK1/2 and p-p38 in different groups, as indicated. Paraffin sections were stained with antibodies against p-ERK1/2 and p-p38. Arrows indicate positive staining. Scale bar, 50 μm. E and F Representative western blot ( E ) and graphical presentations of p-p38/p38 and p-ERK1/2/ERK1/2 protein levels are shown. ** P < 0.01, *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05, ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). G Co-localization of CD44 and p65 in 2 groups, as indicated. Frozen kidney sections were subjected to immunostaining of CD44 (red) and p65 (green). Scale bar, 50 μm. H and I Representative western blot ( H ) and graphical presentations of p-p65, p65, and p-p65/p65 protein levels are shown. ** P < 0.01, *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-wb-testing-2.pngAnti-NF-kB p65/RELA Antibody Picoband®Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1-5: mouse tissue, <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65antigen affinity purified polyclonal antibody (A00284-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 70 kDa. The expected band size for NF-kB p65 is at 70 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig2_html.pngAnti-NF-kB p65/RELA Antibody Picoband®Gene ablation of CD44 attenuates renal tubular cell apoptosis and kidney injury upon IRI. A Experimental design: Wild-type mice and CD44 conventional knockout mice were subjected to IRI or sham, respectively, and euthanized 24 h after IRI. B Scr levels in four groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus wild-type mice upon sham group; ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). C BUN levels in four groups, as indicated. BUN was expressed as milligrams per deciliter. *** P < 0.001 versus wild-type mice upon sham group; ## P < 0.01 versus wild-type mice upon IRI group ( n = 5). D and E Representative western blot of CD44 ( D ) and graphical presentations ( E ) of protein expressional levels are shown. ** P < 0.01 versus wild-type mice upon sham group; ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). F Representative micrographs show the expression of CD44 in different groups, as indicated. Frozen kidney sections were stained with an antibody against CD44. Arrow indicates positive staining. Scale bar, 50 μm. G GO analysis shows CD44 is involved with several important pathways, including MAPK, NF-κB, apoptosis, mitochondria and FAO. H GSEA shows that negative regulation of apoptosis was enriched in CD44 knockout mice versus wild-type mice upon IRI. NES, normalized enrichment score; FDR q-value < 0.25. I–L Representative western blot ( I ) and graphical presentations of J BAX, K BCL2, and L cleaved caspase 3 protein expressional levels are shown. * P < 0.05, *** P < 0.001 versus wild-type mice upon sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). M Representative micrographs show TUNEL assay in different groups, as indicated. Frozen kidney sections were subjected to TUNEL assay. Parrafin sections were performed by immunohistochemistry staining of NGAL. Arrow indicates positive staining. Scale bar, 50 μm. N and O Representative western blot of NGAL ( N ) and graphical presentations ( O ) of protein expressional levels are shown. *** P < 0.001 versus wild-type mice upon sham group; ### P < 0.001 versus wild-type mice upon IRI group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41419_2025_7438_fig1_html.pngAnti-NF-kB p65/RELA Antibody Picoband®CD44 is upregulated in TECs and associated with mitochondrial dysfunction and apoptosis. A UMAP shows cell population in kidneys of sham and IRI at 6 h and day 2. PT proximal tubule, PT-Inj injured PT, PT-R repairing PT, FR-PTC failed repair PT cell, PT-AcInj acute injury PT, DTL descending limb of loop of Henle (LoH), ATL thin ascending limb of LoH, TAL thick ascending limb of LoH, DCT distal convoluted tubule, CNT connecting tubule, PC principal cell of collecting duct, ICA type A intercalated cell of collecting duct, ICB type B intercalated cell of collecting duct, Pod podocyte, EC endothelial cell, Fib fibroblast, Myofib myofibroblast, Ma macrophage (Mφ), B/T immune cell, Uro urothelium. Data from PMID: 36265491. B Graphic presentation of single-cell sequencing analysis shows the expression of CD44 in different cell populations. C Graphic presentation of single-cell sequencing analysis shows the expression of CD44 at different time point. D and E Representative western blot of CD44 ( D ) and graphical presentations ( E ) of protein expressional levels are shown. ** P < 0.01 versus sham group ( n = 5). F Representative micrographs show the expression of CD44 in sham and IRI groups, as indicated. Frozen kidney sections were stained with an antibody against CD44. Arrow indicates positive staining. Scale bar, 50 μm. G Co-localization staining of CD44 and various segment-specific tubular markers in the kidneys of the IRI model. Frozen kidney sections were collected from the mice 1 day after IRI. CD44 (red) and various segment-specific tubular markers (green), including LTL, PNA, and DBA, were detected by immunofluorescence. Arrows indicate positive staining. Scale bar, 50 μm. H GO analysis of CD44-related pathway through STRING ( ). I Co-localization of CD44 and TOMM20 in tubules. Frozen renal sections were subjected to immunostaining of CD44 (red) and TOMM20 (green). Scale bar, 50 μm. J Co-localization of CD44 and cleaved caspase 3 in IRI group. Frozen kidney sections were subjected to immunostaining of CD44 (red) and cleaved caspase 3 (green). Scale bar, 50 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fncel-19-1553309-g009.jpgAnti-NF-kB p65/RELA Antibody Picoband®Activation of TLR4/NF-κB signaling and increased microglial M1-type polarization induces neuronal damage in the tri-culture model. (A) Fluorescence images of CD206 amd CD16 proteins in the microglia in the different groups. (B) Ratio of the relative fluorescence intensities between CD206 amd CD16 proteins in microglia. (C) Fluorescence images of TLR4 protein in neurons in the different groups. (D) Changes in fluorescence intensity of TLR4 protein in neurons in the different groups. (E) Fluorescence images of NF-κB protein in neurons in the different groups. (F) Changes in fluorescence intensity of NF-κB in neurons in the different groups. Data are presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 vs. BM group (one-way ANOVA followed by LSD or Tamhane’s T2). Scale bar = 50 μm. BM: blank media; CHME: conditioned media of hypertensive environment; CORT: corticosterone; TLR4: toll-like receptor 4; NF-κB: nuclear factor-κB.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2025.1553309/full'>40230380</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-fncel-19-1553309-g008.jpgAnti-NF-kB p65/RELA Antibody Picoband®TLR4 inhibitors suppress neuronal apoptosis. (A) Fluorescence images of TUNEL staining in the different groups (red, apoptotic cells; blue, nucleus). (B) The number of apoptotic cells in the different groups. (C) Fluorescence images of apoptosis-related proteins Bcl-2 amd Bax in the different groups. (D) Ratio of the relative fluorescence intensity between Bcl-2 and Bax proteins in the different groups. Data are presented as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 vs. BM group (one-way ANOVA followed by LSD or Tamhane’s T2). Scale bar = 50 μm. BM: blank media; CHME: conditioned media of hypertensive environment; CORT: corticosterone; Bcl-2: B-Cell Lymphoma 2; Bax: Bcl 2-Associated X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2025.1553309/full'>40230380</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41598_2015_article_bfsrep12807_fig3_html.jpgAnti-NF-kB p65/RELA Antibody Picoband®Effects of HMGB1 down-regulation on NF-κB/p65, IκBα and VEGF-C in T24 cells. A1, A2 and A3: The expression of NF-κB/p65 and VEGF-C in the shRNA group was lower than in the other two groups transfected with shNC plasmids or the untransfected controls (all P < 0.05). On the contrary, the expression of IκBα showed an opposite tendency, while no significant differences in the expression of NF-κB/p65, IκBα and VEGF-C in T24 cells were found between the CON and NC groups (all P > 0.05). The display of cropped gels is used to improve the clarity and conciseness of the presentation and all the cropped gels have been run under the same experimental conditions. : The blue areas indicate nuclei stained using 4, 6-diamidino-2-phenylindole (DAPI) and the green areas indicate the nuclear translocation of NF-κB/p65 in T24 cells transfected with shNC plasmids or untransfected and cytoplasmic localization of NF-κB/p65 in cells transfected with shRNA plasmids. The results showed that knockdown of HMGB1 expression inhibited the translocation of NF-κB/p65 from the cytoplasm to the nucleus. : EMSA revealed that the DNA-binding activity of NF-κB/p65 in T24 cells was decreased by HMGB1 knockdown. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep12807'>26239046</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-41598_2023_44590_fig6_html.pngAnti-NF-kB p65/RELA Antibody Picoband®Effect of HRP on the expression of CYP2D6, PKA, CREB, PCREB, IκB, and NF-κB in rats with BCG-induced immune-mediated liver injury. Rats were administered BCG (single intravenous dose of 125 mg/kg BCG) or BCG + HRP (50, 100, or 200 mg ⋅ kg −1 ⋅ d −1 orally for 7 d). Liver proteins were extracted to determine the expression levels of CYP2D6, PKA, CREB, PCREB, IκB, and NF-κB. SDS-PAGE was performed using equal amounts (30 μg) of protein, and western blotting was performed using antibodies against CYP2D6, PKA, CREB, PCREB, i-κB, and NF-κB. The results were normalized to tubulin, GAPDH, or β-actin. The protein expression levels of CYP2D6 ( a ), PKA ( b ), IκB and NF-κB ( c ), CREB, and P-CREB ( d ) in the rat liver were measured by western blotting. The expression levels of CYP2D6, PKA, IκB, NF-κB, CREB, and P-CREB were quantified using the ImageJ software (NIH, Maryland, USA). The data represent the mean ± standard deviation (SD) of three independent experiments. BCG Bacille Calmette-Guerin; HRP Hippophae rhamnoides. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-023-44590-w'>37833431</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-ip-testing-3.jpgAnti-NF-kB p65/RELA Antibody Picoband® Immunoprecipitating NF-kB p65 in HepG2 whole cell lysate. <br>Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>Lane 1: HepG2 whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-NF-kB p65 antibody in HepG2 whole cell lysate, <br>Lane 3: anti-NF-kB p65 antibody (2μg) + HepG2 whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (A00284-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for NF-kB p65 at approximately 65-70 kDa. The expected band size for NF-kB p65 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-fcm-testing-4.jpgAnti-NF-kB p65/RELA Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NF-kB p65 antibody (A00284-1). <br>
Overlay histogram showing Hela cells stained with A00284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF-kB p65 Antibody (A00284-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-if-testing-5.jpgAnti-NF-kB p65/RELA Antibody Picoband® IF analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>
NF-kB p65 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NF-kB p65 Antibody (A00284-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00284-1-rela-primary-antibodies-wb-review-1.pngAnti-NF-kB p65/RELA Antibody Picoband®Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: control group-normal mouse hippocampal tissue lysates,<br>
Lane 2: hippocampal tissue from Alzheimer’s disease model mouse,<br>
Lane 3: hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65antigen affinity purified polyclonal antibody (A00284-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for NF-kB p65 at approximately 65-70 kDa. The expected band size for NF-kB p65 is at 70 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-ribonuclease-3-picoband-trade-antibody-a03115-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03115-1-ribonuclease_3-primary-antibodies-wb-testing-1.jpgAnti-Ribonuclease 3/RNASE3 Antibody Picoband® Western blot analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Jurkat whole cell lysates,<br>Lane 2: rat liver tissue lysates, <br>Lane 3: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribonuclease 3 antigen affinity purified polyclonal antibody (Catalog # A03115-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribonuclease 3 at approximately 18KD. The expected band size for Ribonuclease 3 is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03115-1-Ribonuclease_3-primary-antibodies-IHC-testing-2_1.jpgAnti-Ribonuclease 3/RNASE3 Antibody Picoband® IHC analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1).<br>Ribonuclease 3 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ribonuclease 3 Antibody (A03115-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03115-1-Ribonuclease_3-primary-antibodies-IHC-testing-3_1.jpgAnti-Ribonuclease 3/RNASE3 Antibody Picoband® IHC analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1).<br>Ribonuclease 3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ribonuclease 3 Antibody (A03115-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03115-1-Ribonuclease_3-primary-antibodies-IHC-testing-4_1.jpgAnti-Ribonuclease 3/RNASE3 Antibody Picoband® IHC analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1).<br>Ribonuclease 3 was detected in paraffin-embedded section of human sarcoma tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Ribonuclease 3 Antibody (A03115-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-smoothelin-picoband-trade-antibody-a04895-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04895-smtn-primary-antibodies-wb-testing-1.jpgAnti-Smoothelin/SMTN Antibody Picoband® Western blot analysis of Smoothelin/SMTN using anti-Smoothelin/SMTN antibody (A04895). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human COLO320 whole cell lysates,<br>
Lane 3: human SW620 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smoothelin/SMTN antigen affinity purified polyclonal antibody (Catalog # A04895) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smoothelin/SMTN at approximately 120 kDa. The expected band size for Smoothelin/SMTN is at 99 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-sox3-picoband-trade-antibody-a02255-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02255-1-sox3-primary-antibodies-wb-testing-1.jpgAnti-SOX3 Antibody Picoband® Western blot analysis of SOX3 using anti-SOX3 antibody (A02255-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat heart tissue lysates, <br>Lane 3: mouse brain tissue lysates, <br>Lane 4: mouse heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX3 antigen affinity purified polyclonal antibody (Catalog # A02255-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX3 at approximately 45KD. The expected band size for SOX3 is at 45KD.
https://www.bosterbio.com/products/primary-antibodies/anti-sparcl1-picoband-trade-antibody-a05726-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05726-sparcl1_-primary-antibodies-wb-testing-1.jpgAnti-SPARCL1 Antibody Picoband® Western blot analysis of SPARCL1 using anti-SPARCL1 antibody (A05726). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human U-87MG whole cell lysates,<br>Lane 2: human SHG-44 whole cell lysates,<br>Lane 3: human MDA-MB-231 whole cell lysates,<br>Lane 4: human K562 whole cell lysates,<br>Lane 5: rat C6 whole cell lysates,<br>Lane 6: mouse smooth muscle tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody (Catalog # A05726) at 0.5mg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05726-SPARCL1_-primary-antibodies-IHC-testing-2_1.jpgAnti-SPARCL1 Antibody Picoband® IHC analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).<br>SPARCL1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SPARCL1 Antibody (A05726) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05726-SPARCL1_-primary-antibodies-IHC-testing-3_1.jpgAnti-SPARCL1 Antibody Picoband® IHC analysis of SPARCL1 using anti-SPARCL1 antibody (A05726).<br>SPARCL1 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SPARCL1 Antibody (A05726) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tgf-beta-2-picoband-trade-antibody-a00892-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00892-1-tgf-beta-2-primary-antibodies-wb-testing-1.jpgAnti-TGF beta 2/TGFB2 Antibody Picoband® Western blot analysis of TGF beta 2 using anti-TGF beta 2 antibody (A00892-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCCT tissue lysates, <br>
Lane 2: huamn HCCP tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF beta 2 antigen affinity purified polyclonal antibody (Catalog # A00892-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF beta 2 at approximately 48 kDa. The expected band size for TGF beta 2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00892-1-tgf_beta_2-primary-antibodies-ihc-testing-2.jpg.jpgAnti-TGF beta 2/TGFB2 Antibody Picoband® IHC analysis of TGF beta 2 using anti-TGF beta 2 antibody (A00892-1). <br> TGF beta 2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGF beta 2 Antibody (A00892-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-thrombospondin-2-picoband-trade-antibody-a03253-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03253-1-Thrombospondin_2-primary-antibodies-WB-testing-1_1.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband® Western blot analysis of Thrombospondin 2 using anti-Thrombospondin 2 antibody (A03253-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates, <br>Lane 2: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombospondin 2 antigen affinity purified polyclonal antibody (Catalog # A03253-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombospondin 2 at approximately 160KD. The expected band size for Thrombospondin 2 is at 130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03253-1-41598_2017_8835_fig6_html.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband®Involvement of thrombospondin-2 (THBS2) and microRNA (miR)-128 in hydrogen peroxide-upregulated matrix metalloproteinase-3 (MMP-3) in prostate cancer cells. Comparison of THBS2 ( a ) and miR-128, -134, and -330 ( b ) in PC3 and DU145 prostate cancer cells with or without 50 μM hydrogen peroxide treatment for 48 h through Western blot analysis ( a ) and a real-time RT-PCR ( b ), respectively. Real-time RT-PCR of THBS2 mRNA expression level and ELISA of MMP-3 levels in conditioned media of PC3 transfected with miRNA mimics or inhibitors ( c ). Data are representative of at least three independent experiments and are shown as the mean ± SD. *Student’s t -test * p ≤ 0.05; ** p ≤ 0.001 compared with the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-08835-9'>28831065</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-talin-1-picoband-trade-antibody-a02859-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02859-1-tln1-primary-antibodies-wb-testing-1.jpgAnti-Talin 1/TLN1 Antibody Picoband® Western blot analysis of Talin 1/TLN1 using anti-Talin 1/TLN1 antibody (A02859-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: rat C6 whole cell lysates,<br>
Lane 5: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Talin 1/TLN1 antigen affinity purified polyclonal antibody (Catalog # A02859-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Talin 1/TLN1 at approximately 270 kDa. The expected band size for Talin 1/TLN1 is at 270 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02859-1-jcav11p5032g001.jpgAnti-Talin 1/TLN1 Antibody Picoband®Talin-1 rather than talin-2 is associated with malignancy in NSCLC ( A ) Kaplan-Meier plots of overall survival of patients with squamous carcinoma and adenocarcinoma, stratified by talin-1 and talin-2 expression. ( B ) Western blotting of talin protein levels in fresh NSCLC tissue ( C ) and adjacent normal tissue (P). (C) Normalized quantitative analysis of the wound closure rate in A549 cells transfected with control siRNA or talin-1 siRNA. ( D ) Quantitative analysis of the migration and invasion rates. **P <0.01 compared with the control group, ***P <0.001 compared with the control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7378908/&orig_host=www.ncbi.nlm.nih.gov'>32742451</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-2_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-3_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-4_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-5_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-6_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-7_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-8_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02859-1-Talin_1-primary-antibodies-IHC-testing-9_1.jpgAnti-Talin 1/TLN1 Antibody Picoband® IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).<br>Talin 1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tpr-antibody-a00695-1-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-wb-testing-1_1.jpgAnti-TPR Antibody Picoband® Western blot analysis of TPR using anti-TPR antibody (A00695-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPR antigen affinity purified polyclonal antibody (Catalog # A00695-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPR at approximately 300 kDa. The expected band size for TPR is at 265 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-ihc-testing-2.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-1). <br> TPR was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-ihc-testing-3.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-1). <br> TPR was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-ihc-testing-4.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-1). <br> TPR was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-ihc-testing-5.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-1). <br> TPR was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-if-testing-6.jpgAnti-TPR Antibody Picoband® IF analysis of TPR using anti-TPR antibody (A00695-1) <br> TPR was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-if-testing-7.jpgAnti-TPR Antibody Picoband® IF analysis of TPR using anti-TPR antibody (A00695-1) <br> TPR was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TPR Antibody (A00695-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-1-tpr-primary-antibodies-fc-testing-8.pngAnti-TPR Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TPR antibody (A00695-1). <br> Overlay histogram showing U937 cells stained with A00695-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPR Antibody (A00695-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-he4-picoband-trade-antibody-a02685-4-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02685-4-HE4-primary-antibodies-WB-testing-1_1.jpgAnti-HE4/WFDC2 Antibody Picoband® Western blot analysis of HE4 using anti-HE4 antibody (A02685-4). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human MDA-MB-231 whole cell lysates,<br>Lane 3: human MDA-MB-453 whole cell lysates,<br>Lane 4: rat thymus tissue lysates, <br>Lane 5: mouse testis tissue lysates, <br>Lane 6: mouse thymus tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # A02685-4) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02685-4-wfdc2-primary-antibodies-ihc-testing-5.jpgAnti-HE4/WFDC2 Antibody Picoband®IHC analysis of HE4/WFDC2 using anti-HE4/WFDC2 antibody (A02685-4). <br>HE4/WFDC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HE4/WFDC2 Antibody (A02685-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02685-4-wfdc2-primary-antibodies-ihc-testing-6.jpgAnti-HE4/WFDC2 Antibody Picoband®IHC analysis of HE4/WFDC2 using anti-HE4/WFDC2 antibody (A02685-4). <br>HE4/WFDC2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HE4/WFDC2 Antibody (A02685-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02685-4-HE4-primary-antibodies-IHC-testing-2_1.jpgAnti-HE4/WFDC2 Antibody Picoband® IHC analysis of HE4 using anti-HE4 antibody (A02685-4).<br>HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HE4 Antibody (A02685-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02685-4-HE4-primary-antibodies-IHC-testing-3_1.jpgAnti-HE4/WFDC2 Antibody Picoband® IHC analysis of HE4 using anti-HE4 antibody (A02685-4).<br>HE4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HE4 Antibody (A02685-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02685-4-HE4-primary-antibodies-IHC-testing-4_1.jpgAnti-HE4/WFDC2 Antibody Picoband® IHC analysis of HE4 using anti-HE4 antibody (A02685-4).<br>HE4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-HE4 Antibody (A02685-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/picokine-elisa-kits/human-dkk-4-picokine-trade-elisa-kit-ek0868-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0868.pngHuman DKK-4 / Dickkopf 4 ELISA Kit PicoKine®Human DKK-4 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cdnf-picokine-trade-elisa-kit-ek1656-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1656.pngHuman CDNF ELISA Kit PicoKine®Human CDNF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cdnf-picokine-trade-elisa-kit-ek1657-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1657.pngMouse CDNF ELISA Kit PicoKine®Mouse CDNF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-autotaxin-picokine-trade-elisa-kit-ek1655-boster.html2026-03-24T05:18:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1655.jpgHuman Autotaxin ELISA Kit PicoKine®Human Autotaxin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cystatin-sn-cst1-picokine-trade-elisa-kit-ek1654-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1654.pngHuman Cystatin SN/CST1 ELISA Kit PicoKine®Human Cystatin SN/CST1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-properdin-picokine-trade-elisa-kit-ek1653-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1653.jpgHuman Properdin / Complement Factor P ELISA Kit PicoKine®Human Properdin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-dand5-coco-picokine-trade-elisa-kit-ek1658-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1658.pngHuman DAND5/COCO ELISA Kit PicoKine®Human DAND5/COCO PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-crlf2-tslp-r-picokine-trade-elisa-kit-ek1665-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1665.jpgMouse CRLF2/TSLP R ELISA Kit PicoKine®Mouse CRLF2/TSLP R PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-trkc-picokine-trade-elisa-kit-ek1659-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1659.pngMouse TRKC ELISA Kit PicoKine®Mouse TRKC PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-trkc-picokine-trade-elisa-kit-ek1660-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1660.pngRat TRKC ELISA Kit PicoKine®Rat TRKC PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-smoc2-picokine-trade-elisa-kit-ek1664-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1664.pngHuman SMOC2 ELISA Kit PicoKine®Human SMOC2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-il15ra-picokine-trade-elisa-kit-ek1663-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1663.pngHuman IL15RA ELISA Kit PicoKine®Human IL15RA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-il-38-il1f10-picokine-trade-elisa-kit-ek1662-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1662.pngHuman IL-38/IL1F10 ELISA Kit PicoKine®Human IL-38/IL1F10 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-g1-picokine-trade-elisa-kit-ek1667-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1667.pngHuman Serpin G1 ELISA Kit PicoKine®Human Serpin G1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cdcp1-picokine-trade-elisa-kit-ek1668-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1668.pngHuman CDCP1 ELISA Kit PicoKine®Human CDCP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-mcam-cd146-picokine-trade-elisa-kit-ek1675-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1675.pngHuman MCAM/CD146 ELISA Kit PicoKine®Human MCAM/CD146 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-vitamin-dbp-picokine-trade-elisa-kit-ek1641-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1641.jpgHuman Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®Human Vitamin DBP PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-il-19-picokine-trade-elisa-kit-ek1638-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1638_1.pngHuman IL-19/Interleukin-19 ELISA Kit PicoKine®Human IL-19 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-nt-proanp-nppa-picokine-trade-elisa-kit-ek1627-boster.html2026-03-24T05:18:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1627.pngHuman NT-proANP/NPPA ELISA Kit PicoKine®Human NT-proANP/NPPA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-tff3-picokine-trade-elisa-kit-ek1629-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1629.pngMouse TFF3/ITF ELISA Kit PicoKine®Mouse TFF3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-tff3-picokine-trade-elisa-kit-ek1679-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1679.pngRat TFF3/ITF ELISA Kit PicoKine®Rat TFF3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-agt-serpin-a8-picokine-trade-elisa-kit-ek1465-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1465.jpgHuman AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine®Human AGT PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-fabp2-picokine-trade-elisa-kit-ek1622-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1622_1.pngMouse FABP2 ELISA Kit PicoKine®Mouse FABP2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-fabp3-picokine-trade-elisa-kit-ek1623-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1623.jpgRat FABP3 ELISA Kit PicoKine®Rat FABP3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-he4-wfdc2-picokine-trade-elisa-kit-ek1624-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1624.pngRat HE4/WFDC2 ELISA Kit PicoKine®Rat HE4 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-scgb1a1-uteroglobin-picokine-trade-elisa-kit-ek1635-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1635.jpgMouse SCGB1A1/uteroglobin ELISA Kit PicoKine®Mouse SCGB1A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-scgb1a1-uteroglobin-picokine-trade-elisa-kit-ek1636-ek1636-boster.html2026-03-24T05:18:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1636.pngRat SCGB1A1/uteroglobin ELISA Kit PicoKine®Rat SCGB1A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/anti-aldolase-antibody-a05022-boster.html2026-03-24T05:18:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-aldoa-primary-antibodies-ip-testing-4.jpgAnti-Aldolase ALDOA AntibodyImmunoprecipitation of rabbit anti Aldolase antiserum – Immunoprecipitation performed with 300 ul of antiserum and an equal volume of varied amounts of purified aldolase diluted from a stock solution of ~2.5 mg/ml aldolase in PBS. Antibody/Antigen mixture was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged (see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-aldoa-primary-antibodies-wb-testing-1.jpgAnti-Aldolase ALDOA AntibodyIgG purified antibody to rabbit muscle aldolase (100-1141, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-y-antibody-a06714-1-boster.html2026-03-24T05:18:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06714-1-nfyb-primary-antibodies-ihc-testing-1.jpgAnti-NF-Y NFYB AntibodyImmunohistochemistry. Boster's Anti-NF-Y (B Subunit) antibody was diluted 1:500 to detect NF-YB in human thymus tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
https://www.bosterbio.com/products/primary-antibodies/anti-egfr-antibody-a00023-1-boster.html2026-03-24T05:18:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-1-egfr-primary-antibodies-wb-testing-3.jpgAnti-EGFR AntibodyCombined immunoprecipitation and western blot using anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 µL) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1’. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-1-egfr-primary-antibodies-ihc-testing-1.jpgAnti-EGFR AntibodyImmunohistochemistry of Anti-EGFR Antibody with positive staining.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-1-egfr-primary-antibodies-wb-testing-2.jpgAnti-EGFR AntibodyWestern blot using Boster's anti-EGFR antibody. Lane 1: unstimulated A431 whole cell lysates . Lane 2: EGF stimulated A431 whole cell lysates . Shows detection of a band at ~170 kDa corresponding to human EGFR present in unstimulated and stimulated (50 ng/ml for 15 min) lysates (arrowhead). Loaded: 30µg lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. Primary Antibody: Anti-EGFR at 1:1,000 overnight at 4° C. Secondary Antibody: IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 at 1:10,000 dilution of for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-cdc2-p34-antibody-a30951-boster.html2026-03-24T05:18:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30951-cdk1-primary-antibodies-wb-testing-1.jpgAnti-Cdc2 P34 AntibodyBoster's anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2 by western blot in untreated (Control) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1µM, 6.2µM, 12.5µM and 25.0µM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p16-antibody-a00016-boster.html2026-03-24T05:18:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00016-cdkn2a-primary-antibodies-ihc-testing-1.jpgAnti-p16 Cdkn2a AntibodyBoster's anti-p16 antibody was diluted 1:500 to detect p16 in human prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rfx5-antibody-a05527-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05527-rfx5-primary-antibodies-wb-testing-1.jpgAnti-DNA-binding protein RFX5 RFX5 AntibodyWestern Blot of Rabbit Anti-RFX5 antibody. Lane 1: nuclear extract lysates. Load: 10µg per lane. Primary antibody: RFX5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65 kDa, 46 kDa for RFX5. Other band(s): none.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-beta-antibody-a00118-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00118-ikbkb-primary-antibodies-ihc-testing-1.jpgAnti-IKK beta IKBKB AntibodyImmunohistochemistry of Anti-IKKß antibody. Tissue: human placenta was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-IKKß was diluted 1:500 to detect IKKß in tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00118-ikbkb-primary-antibodies-wb-testing-2.jpgAnti-IKK beta IKBKB AntibodyWestern blot of anti-IKKß antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cox-2-antibody-a00084-1-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-1-ptgs2-primary-antibodies-wb-testing-1.jpgAnti-Cox-2 AntibodyBoster's anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1ß induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-osteopontin-antibody-a00634-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00634-spp1-primary-antibodies-diagram-testing-2.jpgAnti-Osteopontin SPP1 AntibodyOPN is cleaved by MMP to yield 2 fragments, which migrate at 40 kDa (N terminal) and 32 kDa (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs. The epitope that is recognized by Rabbit-anti-Osteopontin is shown in violet. This antibody detects the full length OPN and the 32 kDa fragment. It does not recognize the 40 kDa fragment.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00634-spp1-primary-antibodies-ihc-testing-1.jpgAnti-Osteopontin SPP1 AntibodyRabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00634-spp1-primary-antibodies-wb-testing-3.jpgAnti-Osteopontin SPP1 AntibodyWestern Blot of Rabbit Anti-Osteopontin Antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin [250ng]. Lane 3: MMP-cleaved Osteopontin. Primary Antibody: Anti-Osteopontin at 1:1000 ovenight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-notch-2-antibody-a00518-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-notch2-primary-antibodies-ihc-testing-1.jpgAnti-NOTCH 2 AntibodyBoster's Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-notch2-primary-antibodies-wb-testing-2.jpgAnti-NOTCH 2 AntibodyWestern blot using Boster's anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein. Western Blot analysis was performed for Notch 2 expression using 100µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.
https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-myosin-phospho-s19-phospho-s20-antibody-p11637-1-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/1/p11637-1-myl12a-primary-antibodies-wb-testing-1.jpgAnti-Myosin phospho S19/phospho S20 MYL12A AntibodyWestern Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: HeLa whole cell lysate . Lane 2: HeLa whole cell lysate + smooth muscle recombinant phospho protein. Lane 3: HeLa whole cell lysate + regulatory light chain recombinant phospho protein. Load: 10µg of whole cell lysate + 1.0µg of recombinant protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0µg/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/1/p11637-1-myl12a-primary-antibodies-wb-testing-3.jpgAnti-Myosin phospho S19/phospho S20 MYL12A AntibodyWestern Blot of Rabbit Anti-Myosin pS19/pS20 antibody. Lane 1: Smooth muscle recombinant non-phospho protein. Lane 2: Smooth muscle recombinant phospho protein. Lane 3: Regulatory light chain recombinant non-phospho protein. Lane 4: Regulatory light chain recombinant phospho protein. Load: 1.0 ug of recombinant non-phospho protein, 5.0 ug of recombinant phospho protein. Primary antibody: Mysoin pS19/pS20 antibody at 1.0 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for RLC.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fbxo4-antibody-a08044-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08044-fbxo4-primary-antibodies-if-testing-2.jpgAnti-F-box only protein 4 FBXO4 AntibodyImmunocytochemistry staining of NIH-3T3 cells with Boster’s anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia,https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08044-fbxo4-primary-antibodies-wb-testing-1.jpgAnti-F-box only protein 4 FBXO4 AntibodyWhole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 was detected using Boster’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cul2-antibody-a02986-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02986-cul2-primary-antibodies-ihc-testing-1.jpgAnti-Cullin-2 Cul2 AntibodyBoster's Anti-CUL2 antibody was diluted 1:500 to detect CUL2 in human prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02986-cul2-primary-antibodies-pathway-testing-3.jpgAnti-Cullin-2 Cul2 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cul3-antibody-a00747-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00747-cul3-primary-antibodies-pathway-testing-2.jpgAnti-Cullin-3 Cul3 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00747-cul3-primary-antibodies-ihc-testing-1.jpgAnti-Cullin-3 Cul3 AntibodyBoster's Anti-CUL3 antibody was diluted 1:500 to detect CUL3 in human breast tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parc-antibody-a08481-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08481-cul9-primary-antibodies-pathway-testing-1.jpgAnti-PARC CUL9 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-skp1-antibody-a00476-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-skp1-primary-antibodies-pathway-testing-1.jpgAnti-SKP1 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-anti-ddb1-antibody-a00333-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00333-ddb1-primary-antibodies-pathway-testing-1.jpgAnti-Anti-DDB1 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p48-ddb2-antibody-a01430-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01430-ddb2-primary-antibodies-pathway-testing-1_1.jpgAnti-p48-DDB2 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cand1-antibody-a03609-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03609-cand1-primary-antibodies-pathway-testing-1.jpgAnti-CAND1 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/anti-cand2-antibody-a10530-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10530-cand2-primary-antibodies-pathway-testing-1.jpgAnti-CAND2 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-roc1-antibody-a00524-boster.html2026-03-24T05:18:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00524-rbx1-primary-antibodies-pathway-testing-1.jpgAnti-ROC1 RBX1 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-roc2-antibody-a05620-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05620-rnf7-primary-antibodies-pathway-testing-1.jpgAnti-ROC2 RNF7 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apc11-antibody-a08555-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08555-anapc11-primary-antibodies-pathway-testing-1.jpgAnti-Apc11 ANAPC11 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apc2-antibody-a06153-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06153-anapc2-primary-antibodies-pathway-testing-1.jpgAnti-Apc2 ANAPC2 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
https://www.bosterbio.com/products/primary-antibodies/anti-cybr-antibody-a09710-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09710-cytip-primary-antibodies-wb-testing-1.jpgAnti-Cybr CYTIP AntibodyWestern blot using Boster's anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA. Personal Communication, V. Coppola, NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arfgap3-antibody-a06839-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06839-arfgap3-primary-antibodies-if-testing-1.jpgAnti-ArfGAP3 AntibodyImmunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06839-arfgap3-primary-antibodies-wb-testing-2.jpgAnti-ArfGAP3 AntibodyWestern Blot of Rabbit Anti-ArfGAP3 Antibody. Lane 1 (C): HeLa Whole Cell. Lane 2 (si): HeLa Whole Cell siRNA treated. Load: 10 µg per lane. Primary antibody: ArfGAP3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 57 kDa for endogenous Arf-GAP3. Other band(s): non-specific band ~50kDa.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-morc3-antibody-a06075-1-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-1-morc3-primary-antibodies-wb-testing-1.jpgAnti-Morc3 AntibodyWestern Blot of Rabbit Anti-Morc3 antibody. Lane 1: C-Flag murine embryonic stem cells. Lane 2: C-Flag murine embryonic stem cells doxycycline induced. Load: 35 µg per lane. Primary antibody: mMorc3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 110 kDa for mouse Morc3. Other band(s): Sumoylated Morc runs higher.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb8-antibody-a08344-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08344-abcb8-primary-antibodies-wb-testing-1.jpgAnti-ABCB8 AntibodyWestern Blot of Rabbit anti-ABCB8 antibody. Lane 1: Molecular weight marker. Lane 2: B8 crude membrane prep. Load: 10 µg per lane. Primary antibody: ABCB8 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for ABCB8.
https://www.bosterbio.com/products/primary-antibodies/anti-mmtv-antibody-a30410-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30410-env-primary-antibodies-wb-testing-1.jpgAnti-MMTV env AntibodyWestern Blot of Rabbit anti-MMTV antibody. Lane 1: cell lysate negative control. Lane 2: cell lysate spiked with purified virus. Load: 10 µg per lane. Primary antibody: Mouse Mammary Tumor Virus Surface Protein antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~41.2kDa for MMTV. Other bands: higher bands not unexpected since proteins are made from a larger precursor.
https://www.bosterbio.com/products/primary-antibodies/anti-myeloperoxidase-antibody-a00372-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00372-mpo-primary-antibodies-wb-testing-1.jpgAnti-Myeloperoxidase MPO AntibodyAnti-Myeloperoxidase [Human Leukocytes] detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-il-6-antibody-a00102-3-boster.html2026-03-24T05:18:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00102-3-il6-primary-antibodies-wb-testing-1.jpgAnti-IL-6 AntibodyWestern blot using Boster's Anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated Goat anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00102-3-il6-primary-antibodies-wb-testing-2.jpgAnti-IL-6 AntibodyWestern Blot of Rabbit Anti-IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: Human Rec. IL-6/HeLa Whole Cell Lysate [0.05µg/10µg]. Lane 3: Human Rec. IL-6/HeLa Whole Cell Lysate [0.02µg/10µg]. Lane 4: Human Rec. IL-6/HeLa Whole Cell Lysate [0.01µg/10µg]. Lane 5: HeLa Whole Cell Lysate [10µg]. Primary Antibody: Anti-IL-6 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugate at 1:70,000 for 30mins at RT. Block: BlockOut Buffer . Predicted MW: ~23kDa. Observed MW: ~19 Exposure: 2 seconds.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nedd1-antibody-a06427-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06427-nedd1-primary-antibodies-wb-testing-1.jpgAnti-Protein NEDD1 NEDD1 AntibodyAnti-NEDD1 in Western Blot using Boster Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-aldolase-antibody-a05022-1-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-1-aldoa-primary-antibodies-wb-testing-3.jpgAnti-Aldolase ALDOA AntibodyAnti aldolase antibody – Immunoprecipitation and Western Blot. 300 µl aliquots of whole anti-aldolase antiserum (100-1141) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged(see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-1-aldoa-primary-antibodies-ip-testing-4.jpgAnti-Aldolase ALDOA AntibodyAnti aldolase antibody– Immunoprecipitation- Immunoprecipitation was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 mg/ml) of purified aldolase in PBS. Antibody/Antigen mixture was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel. Gel was stained, destained and imaged(see attached) using standard protocols. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase. Additional higher and lower molecular weight bands correspond to serum proteins.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-1-aldoa-primary-antibodies-wb-testing-2.jpgAnti-Aldolase ALDOA AntibodyWestern Blot of Goat anti-Aldolase Antibody. Lane 1: Hela lysate . Lane 2: HEK293 lysate . Lane 3: Jurkat lysate . Load: 25 µg per lane. Primary antibody: Goat anti-Aldolase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Dk-a-Gt IgG secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicted/Observed size: 39 kDa, 41 kDa for Aldolase.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-1-aldoa-primary-antibodies-wb-testing-1.jpgAnti-Aldolase ALDOA AntibodyIgG purified antibody to rabbit muscle aldolase (100-1141, 200-1141 and 200-1341) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt-phospho-s473-antibody-mp00024-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-fcm-testing-1.jpgAnti-Akt phospho S473 Monoclonal AntibodyFlow Cytometry results of Anti-AKT pS473 (MOUSE) Monoclonal Antibody. The green histogram represents the A431 cells that were stimulated for 15 minutes with 100 ng/mL EGF. The blue histogram shows the untreated A431 cell population, which is bimodal. Both populations were stained with a 1:50 dilution of the Anti-AKT pS473 (MOUSE) Monoclonal Antibody for 30 mins at 4°C. The secondary antibody, Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated was used at a 1:200 dilution for 30 mins at 4°C.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-ihc-testing-2.jpgAnti-Akt phospho S473 Monoclonal AntibodyImmunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-wb-testing-6.jpgAnti-Akt phospho S473 Monoclonal AntibodyWestern Blot of Mouse Anti-AKT pS473 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4° C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent . Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-if-testing-3.jpgAnti-Akt phospho S473 Monoclonal AntibodyImmunofluorescence Microscopy of Mouse Anti-AKTpS473 antibody using STED nanoscopy to evaluate AKT activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved,unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Staining: Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKTpS473 at the cell periphery.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-if-testing-4.jpgAnti-Akt phospho S473 Monoclonal AntibodyHigh resolution STED immunofluorescence nanoscopy of Mouse anti-AKT pS473 antibody. Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min. Antigen retrieval: serum deprivation for 12 h. Primary antibody: AKT pS473 antibody at 10 µg/mL and α-tubulin (cyan) at 1.4 µg/mL for 1 h at RT. Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight™488 anti-Rabbit IgG were used at 1.0 µg/mL for 1h at RT for indirect detection. Localization: AKT pS473 is in the cytoplasm and also organized at the periphery of the cell. Staining: AKT pS473 as red signal with bis-benzimide (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-wb-testing-7.jpgAnti-Akt phospho S473 Monoclonal AntibodyWestern Blot of Mouse Anti-Akt pS473 antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 10 µg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:400 for overnight at 4°C. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-wb-testing-8.jpgAnti-Akt phospho S473 Monoclonal AntibodyWestern Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 µg per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight™649 Conjugated Anti-AKT pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4°C. Predicted/Observed size: 56kDa. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-if-testing-5.jpgAnti-Akt phospho S473 Monoclonal AntibodyImmunofluorescence confocal microscopy of Mouse Anti-AKT pS473 antibody. Tissue: EGF treated A431 cells. Fixation: 0.5% PFA. Antigen retrieval: EGF 15 min. Primary antibody: AKT pS473 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: DyLight 488™ Goat anti-Rabbit IgG, MAb anti-AKT pS473, atto-647N anti-Mouse IgG (Active Motif). at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as red signal with tubulin (cyan).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00024-akt1-primary-antibodies-wb-testing-9.jpgAnti-Akt phospho S473 Monoclonal AntibodyWestern Blot of Mouse Anti-Akt pS473 antibody. A: Lane 1) PDGF stimulated NIH 3T3 cells [10µl]. Lane 2) NIH 3T3 cells [10µl]. Lane 3) Hela whole cell lysate [10µl] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng]. Block: 5% BSA overnight at 4°C. Primary antibody: Boster monoclonal anti-AKT antibody (200-301-268S, lot no. 27843) used at 1:1000 for overnight at 4°C. Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT. Detection: (TMBM-100) for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hef1-antibody-m00838-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00838-nedd9-primary-antibodies-if-testing-1.jpgAnti-HEF1 NEDD9 Monoclonal AntibodyImmunofluorescence microscopy using Boster's Monoclonal anti-HEF1 antibody (clone 2G9) shows detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00838-nedd9-primary-antibodies-wb-testing-2.jpgAnti-HEF1 NEDD9 Monoclonal AntibodyWestern blot using Boster's monoclonal anti-HEF1 antibody (clone 2G9) antibody shows detection of a ~92 kDa band corresponding to HEF1 in MCF7 lysate [arrowhead]. Approximately 35 µg of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye®800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye®800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00838-nedd9-primary-antibodies-wb-testing-3.jpgAnti-HEF1 NEDD9 Monoclonal AntibodyWestern blotting using Boster's monoclonal anti-HEF1 antibody (clone 2G9) shows detection of endogenous HEF1 present in various cell lines [MCF7, HeLa, CHO, 3Y1]. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdcd4-phospho-s457-antibody-mp01105-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01105-pdcd4-primary-antibodies-ihc-testing-1.jpgAnti-Pdcd4 phospho S457 Monoclonal AntibodyBoster Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WAhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01105-pdcd4-primary-antibodies-wb-testing-2.jpgAnti-Pdcd4 phospho S457 Monoclonal AntibodyWestern blot using Boster's Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF [lane 2]. No reactivity is seen for (non-phosphorylated) in unstimulated NIH 3T3 cells [lane 1]. The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4° C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20,000 dilution in blocking buffer for 1 h at 4° C followed by visualization using a Biospectrum® imaging system (UVP).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01105-pdcd4-primary-antibodies-wb-testing-3.jpgAnti-Pdcd4 phospho S457 Monoclonal AntibodyWestern blot using Boster Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1° Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2° Ab Peroxidase Conjugated Rabbit anti-Ms at 1:40,000 in 30 min RT. Bands at ~62 kD and ~32 kD were detected.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat5-phospho-y694-antibody-mp01087-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01087-stat5a-primary-antibodies-ihc-testing-1.jpgAnti-STAT5 phospho Y694 STAT5A Monoclonal AntibodyImmunohistochemistry of Monoclonal Anti-Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti-Stat5pY694 at 20 µg/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01087-stat5a-primary-antibodies-wb-testing-2.jpgAnti-STAT5 phospho Y694 STAT5A Monoclonal AntibodyWestern blot using Boster's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc™ MP 4000 imaging system (Bio-Rad).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01087-stat5a-primary-antibodies-if-testing-3.jpgAnti-STAT5 phospho Y694 STAT5A Monoclonal AntibodyImmunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bin1-antibody-m01551-2-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-2-bin1-primary-antibodies-ip-testing-2.jpgAnti-BIN1 Monoclonal AntibodyImmunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35µg]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-2-bin1-primary-antibodies-if-testing-1.jpgAnti-BIN1 Monoclonal AntibodyImmunofluorescence Microscopy of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-2-bin1-primary-antibodies-wb-testing-3.jpgAnti-BIN1 Monoclonal AntibodyWestern Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 µg per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek1-phosphos222-mek2-phosphos226-antibody-mp00292-boster.html2026-03-24T05:18:56+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdcd4-phospho-s457-antibody-mp01105-1-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01105-1-pdcd4-primary-antibodies-wb-testing-1.jpgAnti-Pdcd4 phospho S457 Monoclonal AntibodyWestern Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoformshttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01105-1-pdcd4-primary-antibodies-elisa-testing-3.jpgAnti-Pdcd4 phospho S457 Monoclonal AntibodyELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 µg per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB
https://www.bosterbio.com/products/primary-antibodies/anti-her3-antibody-a00539-boster.html2026-03-24T05:18:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00539-erbb3-primary-antibodies-wb-testing-1.jpgAnti-HER3 ERBB3 AntibodyCombined immunoprecipitation and western blot of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-b1 for 15' at 100 ng/ml and without (-) the addition of Heregulin-b1. The combination of immunoprecipitation and western blotting was performing using 5 µl of the anti-HER3 antibody for immunoprecipitation followed by western blot detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1’. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00539-erbb3-primary-antibodies-pathway-testing-3.jpgAnti-HER3 ERBB3 AntibodySignaling pathways activated by the ErB3/HER3 Receptor.
https://www.bosterbio.com/products/primary-antibodies/anti-cd97-antibody-a02982-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02982-cd97-primary-antibodies-wb-testing-1.jpgAnti-CD97 antigen CD97 AntibodyWestern blot using Boster's Protein A purified anti-CD97 antibody Lane 1: lysate from cells transfected with control DNA. Lane 2: bone marrow lysates taken from CD97 knockout mice. Lane 3: lysate from COS cells expressing Fc-CD97- (5EGF). Load: 10µL per lane. Primary Antibody: 1:1,000 dilution of the primary antibody was used. Secondary Antibody: Exposure: 10-sec. Results: The formation of the CD97 complex is currently under investigation. A shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. No staining was noted from bone marrow lysates taken from CD97 knockout mice. ~65 kDa appearing in all lanes is not known. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02982-cd97-primary-antibodies-if-testing-3.jpgAnti-CD97 antigen CD97 AntibodyImmunofluorescence Microscopy using Boster's Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2,500 dilution of the primary antibody was used. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf34-antibody-a08108-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08108-rnf34-primary-antibodies-wb-testing-1.jpgAnti-RNF34 AntibodyWestern blot using Boster's Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 µl of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-agap2-antibody-a04771-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04771-agap2-primary-antibodies-wb-testing-1.jpgAnti-AGAP2 AntibodyWestern blot using Boster's Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10,000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-fkbp8-antibody-a03722-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03722-fkbp8-primary-antibodies-wb-testing-1.jpgAnti-FKBP8 AntibodyWestern blot using Boster's protein A purified anti-FKBP8 antibody shows detection of exogenous FKBP8 in 50 µg of HEK293T whole cell lysate (lane 1). The results of peptide competition are shown in lane 2 where no specific staining is noted after the antibody is first incubated for 1h with the immunizing peptide in 5% BLOTTO prior to reaction with the membrane. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BLOTTO at 4° C, overnight. Personal Communication, Olga Aprelikova, CCR-NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-glypican-1-antibody-a03871-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03871-gpc1-primary-antibodies-wb-testing-2.jpgAnti-Glypican-1 AntibodyWestern Blot of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03871-gpc1-primary-antibodies-ihc-testing-1.jpgAnti-Glypican-1 AntibodyImmunohistochemistry of Rabbit anti-Glypican antibody. Tissue: human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Glypican antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-cenexin-1-antibody-a05599-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05599-odf2-primary-antibodies-wb-testing-1.jpgAnti-Cenexin-1 ODF2 AntibodyAnti-Cenexin-1 in Western Blot using Boster Immunochemical's Protein A Purified Anti-Cenexin-1 antibody shows detection of Cenexin-1 in total cell lysates from mouse F9 embryonic carcinoma cells. Arrowheads show detection of Cenexin-1 at approximately 93kDa and Outer dense fiber protein 2 (ODF2) at approximately 70kDa. In personal communication with K. Lee, CCR-NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-nemo-ikk-gamma-antibody-a00874-1-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00874-1-ikbkg-primary-antibodies-wb-testing-1_1.jpgAnti-NEMO/IKK-gamma IKBKG AntibodyWestern Blot of Rabbit anti-NEMO antibody. Lane 1: Opal Pre-stained ladder . Lane 2: Recombinant NEMO protein. Load: 175 ng per lane. Primary antibody: NEMO antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: 30 min at RT. Predicted MW: ~55kDa. Observed MW: ~50kDa for NEMO.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00874-1-ikbkg-primary-antibodies-wb-testing-2_1.jpgAnti-NEMO/IKK-gamma IKBKG AntibodyWestern Blot of Rabbit anti-NEMO antibody. Marker: Opal Pre-stained ladder . Lane 1: Recombinant NEMO protein. Load: 50 ng per lane. Primary antibody: NEMO antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: 30 min at RT. Predicted MW: ~55kDa. Observed MW: ~48kDa for NEMO.
https://www.bosterbio.com/products/primary-antibodies/anti-akt-antibody-a00024-1-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-1-akt1-akt2-akt3-primary-antibodies-wb-testing-1.jpgAnti-AKT AKT1 AntibodyWestern Blot of Anti-AKT (RABBIT) Antibody. M: SuperSignal MW markers. Lane 1: rAKT1. Lane 2: rAKT2. Lane 3: rAKT3. Load: 50 ng per lane. Primary antibody: Anti-AKT (RABBIT) Antibody at 1:1,000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting, 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3.
https://www.bosterbio.com/products/primary-antibodies/anti-setdb2-antibody-a08290-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08290-setdb2-primary-antibodies-wb-testing-1.jpgAnti-Setdb2 AntibodyWestern Blot of Rabbit anti-Setdb2 antibody. Lane 1: 50ng recombinant mouse Setdb2. Lane 2: 50ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 3: 5.0ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 4: 0.5ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 5: HEK293 50mcg. Lane 6: 50ng recombinant mouse Setdb2. Lane 7: 50ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 8: 5.0ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 9: 0.5ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 10: HEK293 50mcg. Primary antibody: Setdb2 antibody at 1:100,000 (lanes 1-5) and 1:25,000 (lanes 6-10) for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80.6 kDa for Setdb2. Other band(s): degradation products, proteolysis products, or nonspecific binding.
https://www.bosterbio.com/products/primary-antibodies/anti-thymidylate-synthase-antibody-a04320-2-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-2-tyms-primary-antibodies-wb-testing-2.jpgAnti-Thymidylate Synthase AntibodyWestern Blot results of Sheep anti-Thymidylate Synthase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder . Lane 2: HeLa WCL . Lane 3: MOLT-4 WCL . Load: 10µg/lane. Primary Antibody: Sheep anti-Thymidylate Synthase at 1µg/mL overnight at 4°C. Secondary Antibody: Donkey anti-Sheep HRP at 1:40,000 for 30min at RT. Blocking: BlockOut for 30 min at RT. Predicted MW: ~36kDa. Observed MW: ~35kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-2-tyms-primary-antibodies-wb-testing-1.jpgAnti-Thymidylate Synthase AntibodyAnti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.
https://www.bosterbio.com/products/primary-antibodies/anti-crp-antibody-fluorescein-conjugated-a00249-3-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00249-3-crp-primary-antibodies-dot-blot-testing-1.jpgAnti-CRP Antibody Fluorescein ConjugatedDot Blot of Goat Anti-Human CRP Antibody FITC. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: 209-1232 Gt-a-hu CRP Fluorescein 1:1000. Blocking Buffer: MB-073 for 1 hour RT.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-6-antibody-m00102-1-boster.html2026-03-24T05:18:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00102-1-il6-primary-antibodies-fcm-testing-3.jpgAnti-IL-6 Monoclonal AntibodyAnti-Human IL-6 Antibody - Flow Cytometry. Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00102-1-il6-primary-antibodies-fcm-testing-1.jpgAnti-IL-6 Monoclonal AntibodyFlow Cytometry of Human anti-IL-6 antibody 209-301-310. Cells: human PBMC. Stimulation: Figure A: unstimulated; Figure B: 1 µg/mL LPS in a protein transport inhibitor for 5 hours. Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00102-1-il6-primary-antibodies-wb-testing-2.jpgAnti-IL-6 Monoclonal AntibodyWestern Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-17a-antibody-m00421-1-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00421-1-il17a-primary-antibodies-wb-testing-1.jpgAnti-IL-17A Monoclonal AntibodyWestern Blot of Mouse Anti-IL-17A antibody. Lane 1: human full length recombinant IL-17A protein. Lane 2: mouse full length recombinant IL-17A protein. Lane 3: rat full length recombinant IL-17A protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17A antibody at 1ug/mL for overnight at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00421-1-il17a-primary-antibodies-wb-testing-2.jpgAnti-IL-17A Monoclonal AntibodyWestern Blot results of Mouse Anti-IL-17A Antibody. Lane 1: Opal Prestained Molecular Weight Ladder . Lane 2: IL-17A. Load: 50ng. Primary Antibody: Mouse Anti-IL-17A at 1µg/mL overnight at 4°C. Secondary Antibody: Rabbit anti-Mouse HRP at 1:40,000 for 30min at RT. Blocking: BlockOut for 30 min at RT.
https://www.bosterbio.com/products/primary-antibodies/anti-il-1-beta-antibody-a00101-2-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-2-il1b-primary-antibodies-wb-testing-2.jpgAnti-IL-1 beta AntibodyWestern Blot of Rabbit anti-Human IL-1ß antibody. Lane 1: Human IL-1ß. Load: 5 ng per lane. Primary antibody: Human IL-1ß antibody at 1:2,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-1ß. Other band(s): Unspecific band at ~35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-2-il1b-primary-antibodies-ihc-testing-1.jpgAnti-IL-1 beta AntibodyImmunohistochemistry of Human IL1 beta antibody. Tissue: human medullary lymph node. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Human IL1 beta antibody. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: cytoplasm. Staining: Close up of medullary lymph node: positive staining in the cytoplasm of circulating macrophages. Neg Ctr (far right) of normal rabbit IgG with pH 6.2 at 40X.
https://www.bosterbio.com/products/primary-antibodies/anti-il-1-alpha-antibody-a01144-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01144-il1a-primary-antibodies-wb-testing-1_1.jpgAnti-IL-1 Alpha AntibodyWestern Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder . Lane 2: IL-1 alpha [50ng]. Lane 3: MEF WC lysate [10ng] . Lane 4: MEF LPS stimulated [10ng]. Blocking: BlockOut Buffer for 30min at RT. Primary Antibody: Anti-IL-1a at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70, 000 for 30min at RT.
https://www.bosterbio.com/products/primary-antibodies/anti-il-9-antibody-a02925-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02925-il9-primary-antibodies-wb-testing-1.jpgAnti-IL-9 AntibodyBoster's anti-Human IL-9 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-9. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
https://www.bosterbio.com/products/primary-antibodies/anti-il-32a-antibody-peroxidase-conjugated-a03286-1-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03286-1-il32-primary-antibodies-wb-testing-1.jpgAnti-IL-32A Antibody Peroxidase ConjugatedWestern blot using Boster's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-esrp-1-2-antibody-m06068-4-boster.html2026-03-24T05:18:58+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ido2-antibody-m06002-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06002-ido2-primary-antibodies-wb-testing-1.jpgAnti-IDO2 Monoclonal AntibodyWestern Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.2kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms.
https://www.bosterbio.com/products/primary-antibodies/anti-il1-beta-antibody-a00101-3-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-il1b-primary-antibodies-if-testing-1.jpgAnti-IL1 beta AntibodyImmunofluorescence microscopy after staining of mouse carotid artery tissue with anti-Mouse IL-1ß antiserum diluted 1:50. Tissue sections were prepared after cryofixation. Reaction occurred at room temperature for 60' followed by washes and reaction with Rhodamine conjugated Gt-a-Rabbit IgG . Tissue was counterstained with bis-benzimide solution at 0.5 mg/ml in PBS for 15 min at room temperature. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1ß staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-1ß KO mouse.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-il1b-primary-antibodies-wb-testing-2.jpgAnti-IL1 beta AntibodyThis antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured IL-1ß will have a 18 kDa band.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-il1b-primary-antibodies-ihc-testing-3.jpgAnti-IL1 beta AntibodyImmunohistochemistry of Rabbit anti-IL1Beta Antibody in Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45” RThttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-13020_2021_562_fig2_html.pngAnti-IL1 beta AntibodyEffects of Pseudoephedrine + emodin on the expression of TNF-α, IL-6, IL-1β, iNOS, IL-10, Arg-1 in LPS‐induced ALI rats. The contents of TNF-α ( A ), IL-6 ( B ), IL-1β ( C ), iNOS ( D ), IL-10 ( E ), Arg-1 ( F ) in the serum and BALF were determined using ELISA. Data were expressed as mean ± S.D. (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. + p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. combined treatment group (5 + 20 mg/kg) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-021-00562-8'>35123524</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-13020_2021_562_fig7_html.pngAnti-IL1 beta AntibodyPseudoephedrine + emodin Inhibited MAPK in LPS-induced acute lung injury in rats. A – D Western blot analysis was performed to detect p-P38, p-ERK1/2 and p-JNK1/2 protein expression. E – I TNF-α, IL-6, IL-1β, IL-10, Arg-1 mRNA expression was determined using Real-time PCR analysis. All data are expressed as mean ± S.D. (n = 3). ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. + p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. Combined treatment group (5 + 20 mg/kg) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-021-00562-8'>35123524</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-cmar-11-10257-g0001.jpgAnti-IL1 beta AntibodyThe main framework to construct the gene pathways dependency network. ( A ) Steps in identifying the genes regulated by ALA-PDT. ( B ) The 46 regulators most highly ranked based on their p-value in the microarray are depicted. ( C ) The KEGG database were used to analyze pathways associated with the inflammatory after ALA-PDT. 133 pathways at 3 hrs(red cycle) after PDT and 140 pathways at 6 hrs (green cycle) identified. Then built the pathway interaction based on the KEGG database on the website and identified the eight signaling pathways ( ). ( D ) Gene expression fold changes of two target genes IL1R1 and IL1β as determined by microarray and real-time q-PCR experiments. The direction and magnitude of fold changes obtained from the real-time q-PCR technique were comparable to those obtained from the microarray technique. P < 0.05 for gene expression fold changes quantified by real-time PCR experiments as determined by two-tailed unpaired Student’s t -test. ( E – G ) IL1β expression was increased 3hrs after ALA-PDT especially in fibroblasts (Vimentin positive cells). Notes: Data were presented as mean ± SEM. *p<0.05; **p<0.01; ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6912005/&orig_host=www.ncbi.nlm.nih.gov'>31849516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-cmar-11-10257-g0002.jpgAnti-IL1 beta Antibody( A, B ) Accelerated solid tumor growth in anti-IL1β-antibody-group and caspase1-inhibitor-group(casp−/− ). Growth curve of SCC tumor ( A , n=10; B , n=10) tumors in SKH-1 mice after ALA-PDT. Note: ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6912005/&orig_host=www.ncbi.nlm.nih.gov'>31849516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-cmar-11-10257-g0003.jpgAnti-IL1 beta Antibody( A ) Surface markers of mCAFs including vimentin, α-SMA and FAP positive. ( B ) CAFs and NIH/3T3 secreted more IL1β than HDF through IHC. Tumor cells including A431 ( C, D ), PECA ( E, F ) and cSCC ( G, H ) expressed more IL1β after ALA-PDT with time, however, the changes have no statistical significance. Fibroblasts including HDF ( C, D ) and NIH/3T3 ( E, F ) expressed more IL1β 48hrs after ALA-PDT, but the difference has not statistical significance. mCAFs expressed more IL1β with time and secreted more IL1β 6hrs, 24hrs and 48hrs after ALA-PDT. Notes: **p<0.01; ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6912005/&orig_host=www.ncbi.nlm.nih.gov'>31849516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-3-cmar-11-10257-g0004.jpgAnti-IL1 beta AntibodyProduction of IL1β occurs through activating NLRP3 inflammasome in an NF-κB-dependent manner. ( A ) Compared with the control, IL1β, NLRP3, caspase-1p20 and ASC all showed increased expression with time, esp in the 48hrs. ( B ) p-p65/p65 ratio increases with time. Control: CAFs were incubated with DMEM and without exposed to the light; Light: CAFs were incubated with DMEM for 5hrs at 37°C and exposed to the light; ALA: CAFs were incubated with ALA (0.5 mM) for 5h at 37°C, but not exposed to the light;12hrs/24hrs/48hrs: CAFs were incubated with ALA (0.5 mM) and exposed to the light. We collected the cell samples in 12hrs/24hrs/48hrs. Notes: *p<0.05; **p<0.01; ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6912005/&orig_host=www.ncbi.nlm.nih.gov'>31849516</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-mouse-il-18-antibody-a00124-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-il18-primary-antibodies-ihc-testing-1.jpgAnti-Mouse IL-18 AntibodyImmunohistochemistry with Rabbit anti-Mouse IL-18 antibody showing IL-18 staining in inflammatory cells of the mucous corium of mouse colon at 20x and 40x. Slide A is a negative control. Slides B and C show staining. Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with mouse anti-IL-18 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-il18-primary-antibodies-if-testing-2.jpgAnti-Mouse IL-18 AntibodyImmunofluorescence microscopy of IL-18 in mouse colon sections. The transversing portion of the large intestine from DSS-exposed (Panel A) and -unexposed mice (Panel B) was excised, rinsed in PBS, and frozen on isopentane cooled with liquid nitrogen. Frozen sections (5 µm) were cut on a Leica CM 1850 cryostat. The slides were fixed for 10 min in 4% paraformaldehyde, air-dried, and incubated for 20 min in PBS supplemented with 10% normal goat serum. Sections were incubated in a 1:50 dilution of Boster's rabbit anti-Mouse IL-18 antibody or 1 µg/ml nonimmune rabbit IgG (not shown) as negative control. The antibodies were diluted in PBS containing 1% bovine serum albumin. After an overnight incubation at 4°C, the sections were washed three times with 0.5% bovine serum albumin in PBS. The sections were then incubated with a secondary goat anti-rabbit antibody conjugated to Alexa488 (Molecular Probes) for 60 min at room temperature in the dark. Nuclei were counterstained blue using 1 µg/100 ml bisbenzimide. After staining, sections were washed and examined with the Leica DM RXA confocal laser scanning system and analyzed. Similar staining will occur with other systems.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-il18-primary-antibodies-neutralization-testing-3.jpgAnti-Mouse IL-18 AntibodyIn-vitro neutralization. Spleens were aseptically removed and cell suspensions were prepared. Cells were washed twice and resuspended in RPMI supplemented with 10% FBS. For cytokine measurement, spleen cells were cultured at 5 mln/mL in 24-well, flat-bottom culture plates in the presence of several dilutions of rabbit anti-murine IL-18 antibody (1:400; 1:200; 1:100; 1:50) and 100 ng/mL of LPS (a phenol-extracted preparation from Escherichia coli 055:B5, Sigma Chemical Co). Cultures were incubated at 37°C in a humidified atmosphere with 5% CO2. At the end of the incubation period, cultures were frozen at -70°C and subjected to 3 freeze-thaw cycles to obtain total cytokine levels. Before assaying, samples were centrifuged for 10 minutes at 10,000g to remove debris.
https://www.bosterbio.com/products/primary-antibodies/anti-il-17f-antibody-peroxidase-conjugated-a02062-boster.html2026-03-24T05:18:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02062-il17f-primary-antibodies-wb-testing-1_1.jpgAnti-IL-17F Antibody Peroxidase ConjugatedWestern Blot of Peroxidase Conjugated Rabbit anti-IL-17F antibody. Lane 1: Mouse IL-17F. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: 30 min at RT. Predicted/Observed size: 16 kDa, 16 kDa for Mouse IL-17F. Other band(s): None.
https://www.bosterbio.com/products/primary-antibodies/anti-apolipoproteinc-iii-antibody-a01416-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01416-apoc3-primary-antibodies-ihc-testing-1_1.jpgAnti-APOLIPOPROTEINC-III APOC3 AntibodyImmunohistochemistry of goat anti-Apolipoprotein C-III antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-III at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-III as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-apolipoproteine-antibody-a00015-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00015-apoe-primary-antibodies-wb-testing-1.jpgAnti-APOLIPOPROTEINE APOE AntibodyWestern Blot of goat Anti Apolipoprotein E Antibody. Lane 1: rat glial whole cell lysate. Load: 40 µg per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4°C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): ~55kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-ap2a-antibody-a30987-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30987-ap2a-primary-antibodies-wb-testing-1.jpgAnti-AP2A ap2a1-1 AntibodyWestern blot using Boster's Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1. Lane 1: HeLa nuclear extract , Lane 2: HeLa , Lane 3: 293 (W09-000-365), Lane 4: A431 , and Lane 5: Jurkat whole cell lysate . In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.
https://www.bosterbio.com/products/primary-antibodies/anti-ring1b-antibody-a01209-1-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-1-rnf2-primary-antibodies-if-testing-1_1.jpgAnti-RING1B RNF2 AntibodyImmunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-1-rnf2-primary-antibodies-wb-testing-2_1.jpgAnti-RING1B RNF2 AntibodyWestern blot using Boster's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B. Lane 1: 3T3 whole cell lysates . Lane 2: U937 . Lane 3: Jurkat . Lane 4: mouse brain . Lane 5: CHO-K1. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
https://www.bosterbio.com/products/primary-antibodies/anti-collagen-type-v-antibody-a02283-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02283-collagen-iv-primary-antibodies-ihc-testing-1_1.jpgAnti-COLLAGEN Type V COL5A1 AntibodyBoster anti collagen V antibody (600-401-107 Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
https://www.bosterbio.com/products/primary-antibodies/anti-collagen-type-vi-antibody-a02226-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02226-collagen-iv-primary-antibodies-ihc-testing-1_1.jpgAnti-Collagen Type VI COL6A1 AntibodyBoster anti collagen VI antibody (600-401-108 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WAhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02226-collagen-iv-primary-antibodies-dot-blot-testing-2_1.jpgAnti-Collagen Type VI COL6A1 AntibodyDot Blot results using Rabbit Anti-Collagen VI Antibody.
Sample: A) Human Collagen VI , B) Bovine/Human Collagen VI .
Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng.
Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr.
Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins.
Blocking: BlockOut® Universal Blocking Buffer at RT for 30mins.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02226-collagen-iv-primary-antibodies-wb-testing-3_1.jpgAnti-Collagen Type VI COL6A1 AntibodyWestern blot results using Rabbit Anti-Collagen Type VI Antibody.
Lane: 1) Human Collagen Type VI reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal prestain molecular weight marker , 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled.
Lanes 2, 4, 6, 8 blank.
Load: 10µg.
Primary Antibody: Rabbit Anti-Collagen Type VI Antibody 1:500 overnight at 2-8°C.
Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated 1:40,000 at RT for 30mins.
Blocking: BlockOut® Universal Blocking Buffer at RT for 1hr.
https://www.bosterbio.com/products/primary-antibodies/anti-frequenin-antibody-a05330-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05330-ncs1-primary-antibodies-if-testing-1.jpgAnti-Frequenin Ncs1 AntibodyHippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Grosse et al., (2000) J. Neurosci. 20: 1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numerous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.
https://www.bosterbio.com/products/primary-antibodies/anti-apc10-antibody-a07065-1-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07065-1-anapc10-primary-antibodies-wb-testing-1.jpgAnti-APC10 ANAPC10 AntibodyWestern Blot of Affinity Purified Rabbit anti-APC10. Lane 1: HeLa nuclear extract . Lane 2: HeLa whole cell lysate . Lane 3: A431 whole cell lysate . Lane 4: Jurkat whole cell lysate . Lane 5: 293 whole cell lysate . All lanes contain 20 µg of lysate or extract. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h to detect human APC10 at ~26 kDa (predicted molecular weight is 21 kDa). After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07065-1-anapc10-primary-antibodies-wb-testing-2.jpgAnti-APC10 ANAPC10 AntibodyWestern Blot of Affinity Purified Rabbit Anti-APC10. Lane 1: HeLa whole cell lysate . Lane 2: HeLa Nuclear lysate . Approximately 20 µg of each lysate was loaded onto a 10% SDS-PAGE gel. Primary Antibody was used at a 1:500 dilution at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa.
https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-glycogen-synthase-1-phospho-s641-antibody-p03512-3-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-3-gys1-primary-antibodies-ihc-testing-1.jpgAnti-Glycogen Synthase 1 phospho S641 GYS1 AntibodyImmunohistochemistry with Anti-Glycogen Synthase antibody. Tissue: Human Prostate. Fixation: formalin-fixed, paraffin-embedded tissue. Antigen retrieval: heat-induced. Primary antibody: 5 µg/ml. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-3-gys1-primary-antibodies-pathway-testing-3.jpgAnti-Glycogen Synthase 1 phospho S641 GYS1 AntibodyDiagram of glycogen synthase as a component of insulin signal transduction pathways.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-3-gys1-primary-antibodies-wb-testing-2.jpgAnti-Glycogen Synthase 1 phospho S641 GYS1 AntibodyAffinity Purified Phospho-specific pS641 antibody to human muscle Glycogen Synthase (GS). Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'. Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'. Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'. Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12µL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG (611-103-122) at 1:5,000 dilution preceded color development using Amersham's substrate system. Other detection methods will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-hif3-alpha-antibody-a03805-1-boster.html2026-03-24T05:18:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03805-1-hif3a-primary-antibodies-wb-testing-1.jpgAnti-Hif3 alpha AntibodyWestern blot using Boster's Affinity Purified Anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A [arrowhead]. Approximately 10 µg of a CoCl2 treated 3T3 cell lysate [lane 1] and control 3T3 cell lysate [lane 2] were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37° C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-bach1-antibody-a01995-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01995-brip1-primary-antibodies-wb-testing-1_1.jpgAnti-BACH1 BRIP1 AntibodyWestern blot using Boster's Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kDa (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate . Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-mad2l1-antibody-a00785-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00785-mad2l1-primary-antibodies-wb-testing-1_1.jpgAnti-MAD2L1 AntibodyWestern blot using Boster's Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat [lane 1] and HeLa [lane 2] whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~ 35µg of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-pc2-antibody-a03388-1-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03388-1-cbx4-primary-antibodies-if-testing-1.jpgAnti-PC2 CBX4 AntibodyBoster's anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2 (see Satijn et al. below). IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Triton X100-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue). Data contributed by Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03388-1-cbx4-primary-antibodies-wb-testing-3.jpgAnti-PC2 CBX4 AntibodyAnalysis shows the detection of human PC2 in probed lysates using Boster's Affinity Purified anti-hPC2 antibody.
https://www.bosterbio.com/products/primary-antibodies/anti-pnk-antibody-a03566-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03566-pnkp-primary-antibodies-wb-testing-1.jpgAnti-PNK PNKP AntibodyWestern blot using Boster's Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 µg of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.
https://www.bosterbio.com/products/primary-antibodies/anti-fbp5b-antibody-a12000-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12000-fbxo43-primary-antibodies-wb-testing-1.jpgAnti-Fbp5B FBXO43 AntibodyWestern blot using Boster's affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute
https://www.bosterbio.com/products/primary-antibodies/anti-fancg-antibody-a03129-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03129-fancg-primary-antibodies-wb-testing-5.jpgAnti-Fanconi anemia group G protein FANCG AntibodyWestern Blot of Rabbit anti-FANCG antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lysate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 10 µg per lane. Primary antibody: FANCG antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 69 kDa for FANCG.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03129-fancg-primary-antibodies-ihc-testing-1.jpgAnti-Fanconi anemia group G protein FANCG AntibodyBoster's Affinity Purified anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03129-fancg-primary-antibodies-wb-testing-2.jpgAnti-Fanconi anemia group G protein FANCG AntibodyWestern blot using Boster's affinity purified anti-FANCG antibody shows detection of a band at ~69 kDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate . Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-whip-antibody-a06521-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06521-wrnip1-primary-antibodies-wb-testing-1.jpgAnti-WHIP WRNIP1 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06521-wrnip1-primary-antibodies-wb-testing-2.jpgAnti-WHIP WRNIP1 AntibodyWestern blot analysis is shown using Boster's anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30µg of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-elg-1-antibody-a06674-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06674-atad5-primary-antibodies-wb-testing-1.jpgAnti-ELG-1 ATAD5 AntibodyWestern blot using Boster's Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 in various cell lysates. Lane 1: HeLa nuclear extract , Lane 2: HeLa , Lane 3: A431 , Jurkat and Lane 5: HEK293 whole cell lysates. Load: ~5µg per lane. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).
https://www.bosterbio.com/products/primary-antibodies/anti-robo-1-antibody-a01530-1-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-elisa-testing-5_1.jpgAnti-ROBO-1 AntibodyELISA results of purified Rabbit anti-Robo-1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-wb-testing-7_1.jpgAnti-ROBO-1 AntibodyWestern Blot of Rabbit anti-Robo-1 antibody. Lane M: Super Signal Molecular Weight Marker. Lane 1: HeLa WCL . Load: 30 µg lysate. Primary antibody: Robo-1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 181 kDa, 181 kDa for Robo-1. Other band(s): lower bands not identified.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-wb-testing-6_1.jpgAnti-ROBO-1 AntibodyWestern Blot of Rabbit anti-ROBO1 antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lysate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 181kDa for ROBO1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-ihc-testing-1_1.jpgAnti-ROBO-1 AntibodyBoster's Affinity Purified anti-ROBO1 antibody was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-wb-testing-2_1.jpgAnti-ROBO-1 AntibodyWestern blot using Boster's Affinity Purified anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-if-testing-3_1.jpgAnti-ROBO-1 Antibody1/50 staining mouse lung tissue sections (adult, frozen 100µm wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-1-robo1-primary-antibodies-if-testing-4_1.jpgAnti-ROBO-1 AntibodyStaining of ROBO1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining. Image from Lindenmeyer MT et al. Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7):e11545, Fig 5.
https://www.bosterbio.com/products/primary-antibodies/anti-gli-3-antibody-a01171-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01171-gli3-primary-antibodies-wb-testing-2_1.jpgAnti-Gli-3 AntibodyWestern Blot of Rabbit anti-Gli-3 antibody. Lane 1: 50 kDa molecular weight marker. Lane 2: 293T cells transfected with CrkL-Flag. Lane 3: 293T cells transfected with human Gli-3. Load: 35 µg per lane. Primary antibody: Gli-3 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 170-190 kDa for hGli-3. Other band(s): Non specific background ~60kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01171-gli3-primary-antibodies-if-testing-4_1.jpgAnti-Gli-3 AntibodyImmunofluorescence Microscopy of Rabbit anti-Gli-3 antibody. Tissue: MCF-7 cell. Antigen retrieval: not required. Primary antibody: Gli-3 antibody and Anti-alpha-Tubulin at 5 µg/mL for 1 h at RT. Secondary antibody: Fluorescein secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear. Staining: Gli-3 staining as red fluorescent signal and Anti-alpha-Tubulin staining as green fluorescent signal using STED.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01171-gli3-primary-antibodies-if-testing-5_1.jpgAnti-Gli-3 AntibodyImmunofluorescence Microscopy of Rabbit anti-Gli-3 antibody. Tissue: MCF-7 cell. Antigen retrieval: not required. Primary antibody: Gli-3 antibody and Anti-alpha-Tubulin at 5 µg/mL for 1 h at RT. Secondary antibody: Fluorescein secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear. Staining: Image (1) shows alpha-Tubulin staining as green fluorescent signal. Image (2) shows Gli-3 staining as red fluorescent signal and Images (3) shows both antibodies fluorescing using STED microscopy.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01171-gli3-primary-antibodies-ihc-testing-1_1.jpgAnti-Gli-3 AntibodyImmunohistochemistry of Rabbit anti-Gli-3 antibody. This image tissue: human glioblastoma. Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Gli-3 antibody at 0.625 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear and smooth muscle. Staining: Gli-3 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01171-gli3-primary-antibodies-wb-testing-3_1.jpgAnti-Gli-3 AntibodyWestern Blot of Rabbit anti-Gli-3 antibody. Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate . Lane 5: mouse lung whole cell lysate . Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: Isoforms at ~170-190kDa and ~80kDa. Lane 2 shows what may be truncated Gli-3 (~80kDa). Other band(s): The strong band at ~50 kDa is unknown.
https://www.bosterbio.com/products/primary-antibodies/anti-gli-2-antibody-a00701-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-gli2-primary-antibodies-ihc-testing-1.jpgAnti-Gli-2 AntibodyBoster's Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-gli2-primary-antibodies-wb-testing-2.jpgAnti-Gli-2 AntibodyWestern blot using Boster's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). Load 25µg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-gli2-primary-antibodies-ihc-testing-3.jpgAnti-Gli-2 AntibodyBoster's Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.
https://www.bosterbio.com/products/primary-antibodies/anti-delta-4-antibody-a00875-2-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-2-dll4-primary-antibodies-elisa-testing-3_1.jpgAnti-DELTA-4 DLL4 AntibodyELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-2-dll4-primary-antibodies-wb-testing-4_1.jpgAnti-DELTA-4 DLL4 AntibodyWestern Blot of Rabbit anti-DELTA-4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate . Lane 2: HUVEK Whole Cell Lysate. Lane 3: Human rDLL4. Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate . Load: 10 µg per lane for Whole Cell Lysates or 50 ng for recombinant protein. Primary antibody: DELTA-4 antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for DELTA-4. Other band(s): Not Identified.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-2-dll4-primary-antibodies-ihc-testing-1_1.jpgAnti-DELTA-4 DLL4 AntibodyBoster's Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-2-dll4-primary-antibodies-wb-testing-2_1.jpgAnti-DELTA-4 DLL4 AntibodyWestern Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue . Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-apc1-phospho-s355-antibody-p03471-2-boster.html2026-03-24T05:19:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03471-2-apc1-primary-antibodies-wb-testing-1.jpgAnti-APC1 phospho S355 mat-2 AntibodyWestern blot using Boster's Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 µg of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (611-103-122) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.
https://www.bosterbio.com/products/primary-antibodies/anti-cdc27-antibody-a03905-2-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03905-2-cdc27-primary-antibodies-wb-testing-1.jpgAnti-CDC27 AntibodyWestern blot using Boster's Affinity Purified anti-cdc27 antibody shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead). Approximately 35µg of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-cdc27-phospho-t244-antibody-p03905-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03905-cdc27-primary-antibodies-dot-blot-testing-2.jpgAnti-CDC27 phospho T244 AntibodyDot Blot of Rabbit Anti-CDC27pT244 Antibody. Dilutions in Columns: (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.7ng, (5) 1.23ng. Tested BSA Peptide Reactivity in Rows: (A) AKT1-BSA, (B) AKT1 pT308-BSA, (C) AKT1 S473-BSA, (D) AKT1 pS473-BSA, (E) CDC27 T244-BSA, (F) CDC27 pT244-BSA, (G) BSA control. Primary Antibody: Anti-CDC27pT244 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP at 1:70,000 at RT for 30mins. Block: BlockOut Buffer .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03905-cdc27-primary-antibodies-wb-testing-1.jpgAnti-CDC27 phospho T244 AntibodyWestern blot using Boster's Affinity Purified anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis. Each lane contains approximately 30 µg of HeLa whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (611-103-122) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.
https://www.bosterbio.com/products/primary-antibodies/anti-gga3-antibody-a03641-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03641-gga3-primary-antibodies-elisa-testing-2.jpgAnti-GGA3 AntibodyELISA results of purified Rabbit anti-GGA3 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03641-gga3-primary-antibodies-wb-testing-3.jpgAnti-GGA3 AntibodyWestern Blot of Rabbit anti-GGA3 antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: LNCaP Lysate . Lane 7: A-172 Lysate . Lane 8: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: GGA3 antibody at 1:5,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (MB-082) for 30 min at RT. Predicted/Observed size: 78 kDa for GGA3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03641-gga3-primary-antibodies-wb-testing-1.jpgAnti-GGA3 AntibodyWestern blot using Boster's Affinity Purified anti-GGA3 antibody shows detection of a band at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red). Approximately 35 µg of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:600. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel - green). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
https://www.bosterbio.com/products/primary-antibodies/anti-noxo1-antibody-a05047-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05047-noxo1-primary-antibodies-wb-testing-3.jpgAnti-NADPH oxidase organizer 1 NOXO1 AntibodyWestern blot using Boster's Affinity Purified anti-NOXO1 antibody shows detection of a band ~50 kDa corresponding to human NOXO1. Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, 3T3, untransfected 293 or WT MEF cells. A 1:1,000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Personal Communication, Zhenggang Liu, NIH, CCR, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05047-noxo1-primary-antibodies-ihc-testing-1.jpgAnti-NADPH oxidase organizer 1 NOXO1 AntibodyBoster's affinity purified anti-NOXO1 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of the lamina propia in human colon epithelium and macrophages at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpan Biosciences, Seattle, WA.
https://www.bosterbio.com/products/primary-antibodies/anti-pin1-antibody-a00467-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00467-pin1-primary-antibodies-wb-testing-5.jpgAnti-PIN1 AntibodyWestern blot using Boster's affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG (611-103-122) was used with a 15 sec exposure time. Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00467-pin1-primary-antibodies-wb-testing-3.jpgAnti-PIN1 AntibodyWestern Blot of Rabbit anti-PIN1 antibody. Lane 1: T98G cells treated with scrambled (scr). Lane 2: T98G cells treated with PIN1 kd1 shRNA. Lane 3: T98G cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T98G cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T98G cells treated with PIN1 kd2 shRNA. Load: 25 µg per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (α-tub) antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00467-pin1-primary-antibodies-wb-testing-4.jpgAnti-PIN1 AntibodyImmunoprecipitation of Rabbit anti-PIN1 antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 µg per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00467-pin1-primary-antibodies-ihc-testing-1.jpgAnti-PIN1 AntibodyImmunohistochemistry of rabbit anti-PIN1 antibody. Tissue: testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PIN1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIN-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-cybr-antibody-a09710-1-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09710-1-cytip-primary-antibodies-wb-testing-1.jpgAnti-Cybr CYTIP AntibodyWestern blot using Boster's affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes. Lane 6 shows direct western blot of knock out mouse. Personal Communication, V. Coppola, NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-pbk1-antibody-a07282-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07282-rsl1d1-primary-antibodies-if-testing-1.jpgAnti-PBK1 RSL1D1 AntibodyImmunofluorescence microscopy of HeLa cells transfected with GFP-PBK1. In the overlay, specific antibody staining is shown to co-localize with recombinant protein. Cells were fixed with methanol prior to staining. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07282-rsl1d1-primary-antibodies-wb-testing-2.jpgAnti-PBK1 RSL1D1 AntibodyWestern blot using Boster's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with null vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1. Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-sipa1-antibody-a05549-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-sipa1-primary-antibodies-ihc-testing-1.jpgAnti-Sipa1 AntibodyBoster's affinity purified anti-Sipa1 antibody was used at 1.25 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-sipa1-primary-antibodies-wb-testing-2.jpgAnti-Sipa1 AntibodyWestern blot using Boster's affinity purified anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 kDa, indicated by the arrowhead, corresponds to recombinant Sipa1. Primary antibody was used at 1:1250. Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-sipa1-primary-antibodies-ihc-testing-3.jpgAnti-Sipa1 AntibodyImmunohistochemistry of rabbit anti-Sipa1 antibody. Tissue: small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Sipa1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-sipa1-primary-antibodies-ihc-testing-4.jpgAnti-Sipa1 AntibodyImmunohistochemistry of rabbit anti-Sipa1 antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Sipa1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sipa-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-thra-antibody-a01775-boster.html2026-03-24T05:19:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01775-thra-primary-antibodies-wb-testing-1_1.jpgAnti-Thyroid hormone receptor alpha THRA AntibodyWestern blot using Boster's affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-smad2-antibody-a00090-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-smad2-primary-antibodies-wb-testing-1.jpgAnti-SMAD2 AntibodyWestern blot using Boster's affinity purified anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF beta. Low levels of staining in control lanes correspond to detection of endogenous Smad2. Pre-incubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The blot presented is askew relative to the molecular weight markers. The expected MW for Smad2 is 52 kDa. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.
https://www.bosterbio.com/products/primary-antibodies/anti-cebp-delta-antibody-a03499-2-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03499-2-cebpd-primary-antibodies-wb-testing-1.jpgAnti-CEBP Delta AntibodyWestern Blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, ~35 kDahttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03499-2-cebpd-primary-antibodies-wb-testing-2.jpgAnti-CEBP Delta AntibodyWestern Blot of Anti-C/EBPd Antibody. Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 h with LPS. Lane 3: C/EBPd-knock-out cells treated 3H with LPS. Load: 35 µg per lane. Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C. Blocking: buffer TBST. ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-abcb5-antibody-a02979-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-abcb5-primary-antibodies-wb-testing-2.jpgAnti-ABCB5 AntibodyWestern blot using Boster's affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight. Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-abcb5-primary-antibodies-ihc-testing-3.jpgAnti-ABCB5 AntibodyImmunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH6. (left, neg control) (right, 20X, 40X). Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-abcb5-primary-antibodies-ihc-testing-4.jpgAnti-ABCB5 AntibodyImmunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH9. (20X, 40X) Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-abcb5-primary-antibodies-ihc-testing-1.jpgAnti-ABCB5 AntibodyImmunohistochemistry of ABCB5 antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: ABCB5 at 1:200. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-smad1-antibody-a00728-2-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00728-2-smad1-primary-antibodies-wb-testing-1.jpgAnti-SMAD1 AntibodyWestern blot using Boster's Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX
https://www.bosterbio.com/products/primary-antibodies/anti-prdm1-blimp1-antibody-a00412-2-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00412-2-prdm1-primary-antibodies-wb-testing-3.jpgAnti-PRDM1 BLIMP1 AntibodyWestern blots using Boster's affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 at ~88kDa. Lane 1: mock Raji cell lysate. Lane 2: overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate. Lane 3: Raji whole cell nuclear extract. Lane 4: endogenous PRDM1/BLIMP1 in human plasma cell nuclear extract. The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00412-2-prdm1-primary-antibodies-ihc-testing-1.jpgAnti-PRDM1 BLIMP1 AntibodyImmunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00412-2-prdm1-primary-antibodies-ihc-testing-2.jpgAnti-PRDM1 BLIMP1 AntibodyImmunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-sprouty-2-antibody-a02089-1-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02089-1-spry2-primary-antibodies-ihc-testing-1_1.jpgAnti-Sprouty-2 SPRY2 AntibodyImmunohistochemistry of Rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4°C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02089-1-spry2-primary-antibodies-ihc-testing-2_1.jpgAnti-Sprouty-2 SPRY2 AntibodyImmunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02089-1-spry2-primary-antibodies-ihc-testing-3_1.jpgAnti-Sprouty-2 SPRY2 AntibodyImmunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-d3-antibody-a01744-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01744-ccnd3-primary-antibodies-ip-testing-3_1.jpgAnti-Cyclin D3 Ccnd3 AntibodyImmunoprecipitation of Rabbit Anti-Cyclin D3. Lane 1: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D3 Mouse mAb. Lane 2: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D2 rabbit pAB. Secondary Antibody: TrueBlot IgG IP beads. Expect: ~32kDa to D3 in lane 1. No reactivity to D2 in lane 2.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01744-ccnd3-primary-antibodies-ihc-testing-1_1.jpgAnti-Cyclin D3 Ccnd3 AntibodyImmunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01744-ccnd3-primary-antibodies-fcm-testing-2_1.jpgAnti-Cyclin D3 Ccnd3 AntibodyFlow Cytometry of Rabbit anti-Cyclin D3 antibody. Cells: mouse B cells+, 14 days post immunization (NP-CGG). Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01744-ccnd3-primary-antibodies-elisa-testing-4_1.jpgAnti-Cyclin D3 Ccnd3 AntibodyELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1µg of conjugate (red line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer , Goat Anti-Rabbit IgG HRP conjugated and TMB substrate .
https://www.bosterbio.com/products/primary-antibodies/anti-jam-a-py280-antibody-a02068-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-f11r-primary-antibodies-wb-testing-2_1.jpgAnti-JAM A pY280 F11R AntibodyWestern Blot of Rabbit anti-JAM A pY280 antibody. Lane 1: SK-CO-15 negative control. Lane 2: SK-CO-15 pervanadate treated positive control. Load: 10 µg per lane. Primary antibody: JAM A pY280 antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: 30 minutes at RT. Predicted/Observed size: ~ 32.5 kDa. JCYIAhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-f11r-primary-antibodies-if-testing-1_1.jpgAnti-JAM A pY280 F11R AntibodyImmunofluorescence Microscopy of Rabbit anti-JAMA pY280 antibody. Tissue: T84 cells (untreated/treated). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: JAMA pY280 antibody at 2 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: JAMA pY280 is along the cell membrane and cell junction. Staining: JAMA pY280 as red fluorescent signal. JCYIA
https://www.bosterbio.com/products/primary-antibodies/anti-nuclear-receptor-ror-gamma-ps203-antibody-a00444-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00444-rorc-primary-antibodies-wb-testing-1.jpgAnti-RORC phospho S203 AntibodyWestern Blot of Rabbit anti-receptor ROR gamma pS203 antibody. Lane 1: Opal PreStained Ladder . Lane 2: Wild-type Mouse Thymus. Lane 3: KO Mouse Thymus. Load: 10 µg per lane. Primary antibody: Receptor ROR gamma pS203 antibody at 1 ug/mL for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 minutes at RT. Predicted/Observed size: 63 kDa for ROR gamma.
https://www.bosterbio.com/products/primary-antibodies/anti-hdac-4-internal-antibody-a00971-2-boster.html2026-03-24T05:19:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00971-2-hdac4-primary-antibodies-wb-testing-1_1.jpgAnti-HDAC-4 (internal) AntibodyWestern Blot of Rabbit anti-HDAC4 antibody. Lane 1: Mouse brain homogenate. Lane 2: Mouse brain homogenate blocked with peptide. Load: 30 µg per lane. Primary antibody: HDAC4 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 140 kDa, 98 kDa, and 55 kDa for HDAC4. Other band(s): HDAC4 splice variants and isoforms.
https://www.bosterbio.com/products/primary-antibodies/anti-huntington-antibody-a00134-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00134-htt-primary-antibodies-wb-testing-1.jpgAnti-Huntington HTT AntibodyWestern Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 350 kDa for Huntington. Other band(s): Huntington splice variants and isoforms.
https://www.bosterbio.com/products/primary-antibodies/anti-protein-kinase-c-delta-binding-protein-antibody-a05919-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05919-cavin3-primary-antibodies-ihc-testing-1.jpgAnti-Protein Kinase C delta-Binding Protein PRKCDBP AntibodyImmunohistochemistry of rabbit anti-Protein Kinase C delta Binding Protein antibody. Tissue: Lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Protein Kinase C delta Binding Protein at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Protein Kinase C delta Binding Protein as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05919-cavin3-primary-antibodies-ihc-testing-3.jpgAnti-Protein Kinase C delta-Binding Protein PRKCDBP AntibodyImmunohistochemistry of rabbit anti-Protein Kinase C delta Binding Protein antibody. Tissue: Placenta. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Protein Kinase C delta Binding Protein at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Protein Kinase C delta Binding Protein as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-hdac-5-internal-antibody-a01230-2-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01230-2-hdac5-primary-antibodies-ihc-testing-1_1.jpgAnti-HDAC-5 (internal) AntibodyImmunohistochemistry of Rabbit anti-HDAC5 antibody. Tissue: Colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC5 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: HDAC5 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/anti-human-serum-albumin-antibody-fluorescein-conjugated-a01245-2-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01245-2-alb-primary-antibodies-dot-blot-testing-1_1.jpgAnti-Human Serum Albumin Antibody Fluorescein ConjugatedDot Blot results of Rabbit Anti-Human Serum Albumin Antibody Fluorescein Conjugate. Dots are Human Serum Albumin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Human Serum Albumin Antibody FITC at 1ug/mL in 1% Fish Gel 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.
https://www.bosterbio.com/products/primary-antibodies/anti-nag-1-antibody-peroxidase-conjugated-a01583-3-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01583-3-gdf15-primary-antibodies-wb-testing-1_1.jpgAnti-Nag-1 GDF15 Antibody Peroxidase ConjugatedWestern blot using Boster's affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01583-3-gdf15-primary-antibodies-ihc-testing-3_1.jpgAnti-Nag-1 GDF15 Antibody Peroxidase ConjugatedImmunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01583-3-gdf15-primary-antibodies-ihc-testing-4_1.jpgAnti-Nag-1 GDF15 Antibody Peroxidase ConjugatedImmunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.
https://www.bosterbio.com/products/primary-antibodies/anti-stim2-antibody-a02345-1-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02345-1-stim2-primary-antibodies-ihc-testing-1_1.jpgAnti-Stromal interaction molecule 2 Stim2 AntibodyImmunohistochemistry of Sheep anti-STIM2 antibody. Tissue: Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: STIM2 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: STIM2 as precipitated red signal with hematoxylin purple nuclear counterstain.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt1-antibody-m00018-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-sirt1-primary-antibodies-wb-testing-1.jpgAnti-SIRT1 Monoclonal Antibody Western blot analysis of SIRT1 using anti-SIRT1 antibody (M00018). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified monoclonal antibody (Catalog # M00018) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-ihc.jpgAnti-SIRT1 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human kidney, using SIRT1 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-if.jpgAnti-SIRT1 Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using SIRT1 Antibody .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-icc1.jpgAnti-SIRT1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erk2-antibody-m00030-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-wb.jpgAnti-ERK2 Monoclonal AntibodyWestern blot analysis of ERK2 in (1) A431 cell lysate; (2) HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-wb7.jpgAnti-ERK2 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc9.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc10.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human renal cancer, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc7.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Rat lung, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc11.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Mouse ovary, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc8.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-ihc.jpgAnti-ERK2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using ERK2 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-icc1.jpgAnti-ERK2 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccr2-ckr2-m00158-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-ccr2-primary-antibodies-wb-testing-1.jpgAnti-CCR2/CKR2 Rabbit Monoclonal AntibodyWestern blot analysis of CCR2 using anti-CCR2 antibody (M00158). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human K562 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified monoclonal antibody (M00158) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42 kDa. The expected band size for CCR2 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-icc2.jpgAnti-CCR2/CKR2 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-ihc.jpgAnti-CCR2/CKR2 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human tonsil, using CCR2/CKR2 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-icc1.jpgAnti-CCR2/CKR2 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beclin-1-antibody-m00327-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-wb.jpgAnti-Beclin 1 BECN1 Monoclonal AntibodyWestern blot analysis of Beclin 1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-ihc.jpgAnti-Beclin 1 BECN1 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human breast cancer, using Beclin 1 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-if.jpgAnti-Beclin 1 BECN1 Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using Beclin 1 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-histone-h3-t3-antibody-mp06819-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp06819-wb.jpgAnti-Phospho-Histone H3 (T3) H3F3A Monoclonal AntibodyWestern blot analysis of Phospho-Histone H3 (Thr3) in HeLa cell lysates treated with FBS + Calyculin A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp06819-ihc.jpgAnti-Phospho-Histone H3 (T3) H3F3A Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human tonsil, using Phospho-Histone H3 (T3) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-egfr-erbb-1-antibody-m00023-2-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-41419_2017_bfcddis2017546_fig3_html.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyEffects of PCE on the expression changes of Mig-6 and EGFR in fetal long-bone hypertrophic chondrocytes. ( a ) ISH of Mig-6 in hypertrophic chondrocytes. ( b ) Immunostaining of Mig-6 in hypertrophic chondrocytes. ( c ) Immunostaining of EGFR in hypertrophic chondrocytes. ( d ) Serum corticosterone (CORT) concentration of fetal rats (ng/ml). ( e ) Quantification of Mig-6 ISH (fluorescence intensity). ( f ) Quantification of Mig-6 immunostaining (optical density). ( g ) Quantification of EGFR immunostaining (optical density). n =5 per group obtained from different litters. Three random fields/section for quantitative. Data are shown as the mean±S.D. * P <0.05, ** P <0.01 versus control (ANOVA) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2017546'>29072695</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-41419_2017_bfcddis2017546_fig4_html.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyEffects of corticosterone (250–1250 nM) with/without siRNA (Mig-6, EGFR) for 48 h on rats primary chondrocytes terminal differentiation and apoptosis. ( a and b ) mRNA expression of mitogen-inducible gene 6 (Mig-6) and EGFR after corticosterone and Mig-6 siRNA treatment, ( c ) Protein expression of Mig-6, EGFR, phosphorylated EGFR (P-EGFR), c-Jun N-terminal kinase (JNK) and Phosphorylated JNK (P-JNK) detected by western blotting after corticosterone and Mig-6 siRNA treatment. ( d – h ) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (relative grayscale). ( i – k ) mRNA expression of runt-related transcription factor 2 (Runx2), collagen type X (Col-X) and matrix metalloproteinases-13 (MMP-13) after corticosterone and Mig-6 siRNA treatment. ( l and m ) Apoptotic analysis detected by Annexin V/PI after corticosterone and Mig-6 siRNA treatment. ( n ) Protein expression of EGFR, JNK and P-JNK detected by western blotting after EGFR siRNA treatment. ( o ) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (Relative grayscale). ( p ) mRNA expression of Runx2, Col-X and MMP-13 after EGFR siRNA treatment. ( q ) Apoptotic analysis detected by Annexin V/PI after EGFR siRNA treatment. Data are shown as the mean±S.D. of results from three experiments. * P <0.05, ** P <0.01 versus control; # P <0.05, ## P <0.01 versus CORT treatment group. (A-M, ANOVA; O-R, t test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2017546'>29072695</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-41419_2017_bfcddis2017546_fig6_html.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyThe proposed schematic model of the present study. Col-X, collagen type X; EGFR, epidermal growth factor receptor; JNK, c-Jun N-terminal kinase; Mig-6, mitogen-inducible gene 6; MMP-13, matrix metallopeptidase 13; Runx2, runt-related transcription factor 2 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2017546'>29072695</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-egfr-primary-antibodies-wb-testing-1.jpgAnti-EGFR (ErbB 1) Monoclonal Antibody Western blot analysis of EGFR using anti-EGFR antibody (M00023-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human U-87MG whole cell lysates,<br>
Lane 5: rat liver tissue lysates,<br>
Lane 6: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified monoclonal antibody (Catalog # M00023-2) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc7.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc8.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc9.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc10.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc11.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Mouse intestine, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-ihc.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human stomach cancer, using EGFR (ErbB 1) Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-icc1.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-icc2.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-icc3.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-2-icc4.jpgAnti-EGFR (ErbB 1) Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hip1-antibody-m02242-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02242-hip1-primary-antibodies-wb-testing-1_1.jpgAnti-HIP1 Monoclonal Antibody Western blot analysis of HIP1 using anti-HIP1 antibody (M00346-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human RT4 whole cell lysates, <br>
Lane 4: human SH-SY5Y whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1 antigen affinity purified monoclonal antibody (M00346-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-myc-antibody-m00026-1-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-1-wb7.jpgAnti-c-Myc Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-1-ihc.jpgAnti-c-Myc Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human lung cancer, using c-Myc Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-1-icc1.jpgAnti-c-Myc Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-1-if.jpgAnti-c-Myc Monoclonal AntibodyImmunofluorescent analysis of HeLa cells, using c-Myc Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-1-wb.jpgAnti-c-Myc Monoclonal AntibodyWestern blot analysis of c-Myc expression in (1) Jurkat cell lysate;(2) K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-histone-h3-s10-antibody-mp12477-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp12477-histone_h3-primary-antibodies-wb-testing-1.jpgAnti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal Antibody Western blot analysis of Phospho-Histone H3 (S10) using anti-Phospho-Histone H3 (S10) antibody (MP12477). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human 293T whole cell lysates, <br>
Lane 5: rat RH35 whole cell lysates, <br>
Lane 6: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-Histone H3 (S10) antigen affinity purified monoclonal antibody (MP12477) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-Histone H3 (S10) at approximately 17 kDa. The expected band size for Phospho-Histone H3 (S10) is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp12477-ihc.jpgAnti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H3 (S10) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atm-antibody-m00014-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00014-atm-primary-antibodies-wb-testing-1.jpgAnti-ATM Monoclonal Antibody Western blot analysis of ATM using anti-ATM antibody (M00014). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (Catalog # M00014) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATM at approximately 351 kDa. The expected band size for ATM is at 351 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00014-ihc.jpgAnti-ATM Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human breast cancer, using ATM Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00014-if.jpgAnti-ATM Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using ATM Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erbb2-her2-antibody-m00010-1-boster.html2026-03-24T05:19:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-wb7.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-wb.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyWestern blot analysis of ErbB2 (HER2) expression in SKBR-3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-ihc7.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-ihc.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human breast carcinoma, using ErbB2 (HER2) Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-ihc9.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-1-if.jpgAnti-ErbB2 (HER2) Monoclonal AntibodyImmunofluorescent analysis of SKBR cells, using ErbB2 (HER2) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi-3-kinase-p110-alpha-antibody-m00029-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00029-wb7.jpgAnti-PI 3 kinase p110 alpha Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00029-wb.jpgAnti-PI 3 kinase p110 alpha Monoclonal AntibodyWestern blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.Western blot analysis of PI 3 kinase p110 alpha expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00029-wb8.jpgAnti-PI 3 kinase p110 alpha Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00029-wb9.jpgAnti-PI 3 kinase p110 alpha Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-notch1-antibody-m00033-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00033-notch1-primary-antibodies-wb-testing-1_1.jpgAnti-Notch1 Monoclonal Antibody Western blot analysis of NOTCH1 using anti-NOTCH1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Human 293T whole cell lysates,<br>
Lane 2: Human K562 whole cell lysates,<br>
Lane 3: Human Jurkat whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOTCH1 at approximately 110 kDa. The expected band size for NOTCH1 is at 273 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00033-NOTCH1-IHC-test-2.jpgAnti-Notch1 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00033-NOTCH1-IF-test-3.jpgAnti-Notch1 Monoclonal AntibodyImmunofluorescent analysis of HeLa cells, using Notch1 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00033-icc1.jpgAnti-Notch1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h4-acetyl-k16-antibody-m14495-3-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-3-histone_h4-primary-antibodies-wb-testing-1.jpgAnti-Histone H4 (acetyl K16) HIST1H4A Monoclonal AntibodyWestern blot analysis of Histone H4 using anti-Histone H4 antibody (M14495-3). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U2OS whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human HEL whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H4 antigen affinity purified monoclonal antibody (M14495-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H4 at approximately 15 kDa. The expected band size for Histone H4 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-3-ihc.jpgAnti-Histone H4 (acetyl K16) HIST1H4A Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded rat lung, using Histone H4 (acetyl K16) Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-3-if.jpgAnti-Histone H4 (acetyl K16) HIST1H4A Monoclonal AntibodyImmunofluorescent analysis of HeLa cells treated with TSA, using Histone H4 (acetyl K16) Antibody .
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jak2-antibody-m00027-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-images_medium_ao4c00710_0003.gifAnti-JAK2 Monoclonal AntibodyIn vitro cytotoxicity of CLCS nanoliposome. (a) Confocal fluorescence pictures of cell uptake of nanoliposomes (blue: cell nucleus, and red: LCS or CLCS). Scale bar: 500 nm. (b) Quantification of cell uptake of nanoliposomes through flow cytometry at different time points. (c) Representative flow cytometric images of C1498 cell uptake of nanoliposomes after being treated with LCS and CLCS for 4 and 8 h. (d) Dead/live staining of C1498 cells after various treatments (green: live cells, and red: dead cells). (e) Apoptosis/necrosis analysis of C1498 cells after multifarious treatments by flow cytometry. (f) Quantification of apoptotic cell after various treatments. (g) Western blotting analysis of JAK2/STAT3 expression in AML cells after sorts of treatments. (h) Cell viability of C1498 cells after multiple treatments. *p < 0.05, **p < 0.01, and ***p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pubs.acs.org/doi/abs/10.1021/acsomega.4c00710'>39281932</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027_12951_2025_3576_fig10_html.pngAnti-JAK2 Monoclonal Antibody Validation of the mechanism by which FA-PPI-Ms reverse M1/M2 imbalance. ( A-B ) qRT-PCR analysis of CD86 and CD206 mRNA expression on activated RAW264.7 cells treated with different formulations; ( C-D ) Flow cytometric histograms of proportion of M1and M2 macrophages in different formulations; ( E-F ) Quantitative analysis of proportion of M1 and M2 macrophages in different formulations; ( G-H ) Representative fluorescence images of CD86 and CD206 staining of values with varying formulations, scale bar = 50 μm; ( I-J ) Analysis of relative fluorescence intensity in ( G-H ); ( K-L ) qRT-PCR analysis of JAK2 and STAT3 mRNA expression on activated RAW264.7 cells treated with different formulations; ( M ) Western blot analysis of JAK2-STAT3 signalling axis-related protein expression. ( N-O ) Semi-quantitative analysis of p-JAK2/ JAK2 and p-STAT3/ STAT3 levels by Image J, Data are presented as mean ± SD ( n = 3). One-way ANOVA followed by Bonferroni’s test was used. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03576-8'>40660236</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-jak2-primary-antibodies-wb-testing-1.jpgAnti-JAK2 Monoclonal Antibody Western blot analysis of JAK2 using anti-JAK2 antibody (M00027). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: rat NRK whole cell lysates,<br>
Lane 4: rat PC-12 whole cell lysates,<br>
Lane 5: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (Catalog # M00027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027_12951_2025_3576_fig9_html.pngAnti-JAK2 Monoclonal AntibodyTranscriptomic analysis elucidated the mechanism of action of FA-PPI-Ms in the treatment of RA. ( A ) Volcano plot of significantly differentially expressed genes between the model group and the FA-PPI-Ms group, where blue circles represent downregulated genes and red circles represent upregulated genes; ( B ) Heatmap of significantly differentially expressed genes in cells of the model group and the FA-PPI-Ms group, where deeper blue indicates lower expression levels and deeper red indicates higher expression levels; ( C ) Histogram of GO functional annotation of significantly differentially expressed genes between the model group and the FA-PPI-Ms group; ( D ) Histogram of GO functional enrichment of significantly different genes between the model group and the FA-PPI-Ms group; ( E ) Bubble plot of GO functional enrichment of significantly differentially expressed genes between the model group and the FA-PPI-Ms group; ( F ) Histogram of KEGG pathway enrichment of genes with significant differences between the model group and the FA-PPI-Ms group; ( G-H ) To validate the transcriptomics results, qRT-PCR was employed to measure the mRNA expression levels of JAK ( G ) and STAT3 ( H ) in RAW264.7 cells; ( I ) Western blot was utilized to assess the expression levels of proteins associated with the JAK2-STAT3 signaling pathway; ( J-K ) Statistical analysis of the ratios of p-JAK2/JAK2 ( J ) and p-STAT3/STAT3 ( K ) was conducted. Data are presented as mean ± SD ( n = 3), * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03576-8'>40660236</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-jak2-primary-antibodies-ihc-testing-2.jpgAnti-JAK2 Monoclonal Antibody IHC analysis of JAK2 using anti-JAK2 antibody (M00027). <br>
JAK2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAK2 Antibody (M00027) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-jak2-primary-antibodies-if-testing-3_1.jpgAnti-JAK2 Monoclonal Antibody IF analysis of JAK2 using anti-JAK2 antibody (M00027) and anti-Beta Tubulin antibody (M01857-3).<br>
JAK2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-JAK2 Antibody (M00027) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-jak2-primary-antibodies-wb-testing-2.pngAnti-JAK2 Monoclonal Antibody Western blot analysis of JAK2 using anti-JAK2 antibody (M00027). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Normal group-rat colon tissue lysates, <br>
Lane 2: Model group-rat colon tissue lysates, <br>
Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br>
Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br>
Lane 5: Triditional Chinese medicine treatment (high dose)-rat colon tissue lysates, <br>
Lane 6: Western medicine treatment-rat colon tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK2 antigen affinity purified monoclonal antibody (Catalog # M00027) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for JAK2 at approximately 131 kDa. The expected band size for JAK2 is at 131 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h4-acetyl-k5-antibody-m14495-4-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-4-wb.jpgAnti-Histone H4 (acetyl K5) HIST1H4A Monoclonal AntibodyWestern blot analysis of Histone H4 (acetyl K5) expression in (1) Untreated HeLa HeLa cell lysate; (2) TSA treated HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-4-ihc.jpgAnti-Histone H4 (acetyl K5) HIST1H4A Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human colon, using Histone H4 (acetyl K5) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p16-ink-antibody-m00016-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-wb7.jpgAnti-p16 INK Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-wb.jpgAnti-p16 INK Monoclonal AntibodyWestern blot analysis of p16 INK expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-wb8.jpgAnti-p16 INK Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-ihc.jpgAnti-p16 INK Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human gastric, using p16 INK Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-icc2.jpgAnti-p16 INK Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-icc1.jpgAnti-p16 INK Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-ip7.jpgAnti-p16 INK Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcr4-antibody-m00031-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-wb.jpgAnti-CXCR4 Monoclonal AntibodyWestern blot analysis of CXCR4 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-wb7.jpgAnti-CXCR4 Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-wb8.jpgAnti-CXCR4 Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-wb9.jpgAnti-CXCR4 Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-wb10.jpgAnti-CXCR4 Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-ihc.jpgAnti-CXCR4 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human cervical carcinoma, using CXCR4 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-if.jpgAnti-CXCR4 Monoclonal AntibodyImmunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00031-icc1.jpgAnti-CXCR4 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-src-y419-antibody-mp00107-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00107-wb.jpgAnti-Phospho-Src (Y419) Monoclonal AntibodyWestern blot analysis of Phospho-Src (Y419) expression in A431 cell lysate treated with pervanadate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2a-acetyl-k9-antibody-m16777-3-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-3-wb.jpgAnti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal AntibodyWestern blot analysis of Histone H2A (acetyl K9) expression in HeLa cell lysate treated Trichostatin A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-3-ihc.jpgAnti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded mouse brain, using Histone H2A (acetyl K9) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-jnk1-2-3-t183-t183-t221-antibody-mp02608-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-wb7.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-wb.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyWestern blot analysis of JNK1/2/3 phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-12951_2025_3154_fig4_html.pngAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyGenome-wide RNA-sequencing of KPC cells treated with Cel in vitro. (A) Volcano plots of the total differentially expressed genes (DEGs) between Cel-treated group and PBS group. (B) Heat map of the specific DEGs. (C) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. (D) The expression levels of p-AKT, p-ERK, p-JNK, and p-P38 were detected in KPC cells treated with Cel at the 0 h, 0.5 h, 2 h, and 4 h by western blotting <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03154-y'>40050985</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-feb413914-fig-0010-m.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyEffects of OPN and OVE on activation of MAPK signaling pathways in LPS-stimulated RAW264.7 cells. Expression levels of p-ERK, ERK, p-p38, p-38, p-JNK, and JNK were detected in the same samples for COX-2 detection after 24 h of LPS stimulation. (A) OVE treatment. (B) OPN treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ***P < 0.001 and ****P < 0.0001, compared to the LPS control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/2211-5463.13914'>39455284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-icc1.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-icc2.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-if.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyImmunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-p-jnk1-2-3-primary-antibodies-ihc-testing-1_1.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyIHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (MP02608). <br>P-JNK1/2/3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-JNK1/2/3 Antibody (MP02608) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-p-jnk1-2-3-primary-antibodies-ihc-testing-2_1.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyIHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (MP02608). <br>P-JNK1/2/3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-JNK1/2/3 Antibody (MP02608) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-p-jnk1-2-3-primary-antibodies-ihc-testing-3_1.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyIHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (MP02608). <br>P-JNK1/2/3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-JNK1/2/3 Antibody (MP02608) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02608-p-jnk1-2-3-primary-antibodies-ihc-testing-4_1.jpgAnti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal AntibodyIHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (MP02608). <br>P-JNK1/2/3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-JNK1/2/3 Antibody (MP02608) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akt1-antibody-m00024-1-boster.html2026-03-26T05:20:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-41598_2017_12907_fig4_html.jpgAnti-AKT1 Monoclonal Antibody( a ) Immunofluorescence staining of cavernous tissue using an antibody against p-Akt1(Tyr308) in the CO, MED and EGF groups. ( b ) Immunohistochemistry staining of cavernous tissue was performed with an antibody against Akt1 in three groups (magnification: ×400 scale bar: 20 μm). ( c,d ) Western blot analysis of p-Akt1 (Tyr308) and Akt1 expression in the three groups. Data in the bar graphs are expressed as the means ± SD of 5~7 rats. *p < 0.05 vs the CO group. p-Akt, phosphor-protein kinase B; CO, normal control rats; MED, metabolic syndrome-related erectile dysfunction rats; EGF, MED rats treated with epithelial growth factor; SD, standard deviation. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-12907-1'>29044143</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-akt1-primary-antibodies-wb-testing-1.jpgAnti-AKT1 Monoclonal Antibody Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: human MCF-7 whole cell lysates,<br>
Lane 6: human SiHa whole cell lysates,<br>
Lane 7: human 293T whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 56 kDa. The expected band size for AKT1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-16-1582677-g010.jpgAnti-AKT1 Monoclonal AntibodyTreatment with quercetin in PASMC proliferation and antioxidation under hypoxia. (A, B) Quercetin in cell proliferation were assessed using Ki67 immunofluorescence and quantitative evaluation in hypoxia-induced PASMCs (n = 3, scale bar = 100 μm). (C–H) Primitive Western blots and quantitative densities of PCNA, p-PI3K, PI3K, p-AKT1 Ser473, AKT1 with or without 740Y-P(10 μM), LY294002(10 μM), or quercetin (18 μM) in PASMCs under 3% O 2 for 24 h (I–L) Quantitative evaluation of SOD and GSH-Px activities and GSH and MDA contents in 3% O 2 -induced PASMCs. n = 3. All data represent mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + QCT-18 μM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-akt1-primary-antibodies-wb-testing-2.jpgAnti-AKT1 Monoclonal Antibody Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: rat C6 whole cell lysates,<br>
Lane 4: rat RH35 whole cell lysates,<br>
Lane 5: mouse liver tissue lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: Mouse Neuro-2a whole cell lysates,<br>
Lane 8: Mouse HEPA1-6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 56 kDa. The expected band size for AKT1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-12-683613-g009.jpgAnti-AKT1 Monoclonal AntibodyEffect of Lactucin on the activation of signaling pathways. (A) The whole-cell lysates were extracted for immunoblotting to determine the level of iNOS, COX-2. (B, C) The whole-cell lysates were extracted for immunoblotting to determine the levels of phospho- or total MAPKs (ERK, p38, and JNK) and AKT identified based on their antibodies. Data are shown as mean ± SD for each group (* p < 0.05 with the LPS Group, n = 3. Normal Group: RAW264.7 cells without LPS activation).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.683613/full'>33995112</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-1-AKT1-IHC-test-2.jpgAnti-AKT1 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-16-1582677-g009.jpgAnti-AKT1 Monoclonal AntibodyTreatment with eriocitrin in PASMC proliferation and antioxidation under hypoxic conditions. (A, B) Eriocitrin in cell proliferation were assessed using Ki67 immunofluorescence and quantitative evaluation in hypoxia-induced PASMCs (n = 3, scale bar = 100 μm). (C–H) Primitive Western blots and quantitative densities of PCNA, p-PI3K, PI3K, p-AKT1 (Ser473), AKT1 with or without 740Y-P (10 μM), LY294002 (10 μM), or eriocitrin (11 μM) in PASMCs under 3% O 2 for 24 h (I–L) Quantitative evaluation of SOD and GSH-Px activities and GSH and MDA contents in 3% O 2 -induced PASMCs. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + ERI-11 μM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-1-AKT1-IF-test-3.jpgAnti-AKT1 Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using AKT1 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-16-1582677-g008.jpgAnti-AKT1 Monoclonal AntibodyEriocitrin and quercetin are responsible for anti-proliferation by targeting the PI3K protein in PASMCs under hypoxic conditions. ERI, eriocitrin; QCT, quercetin. (A, B) Primitive bands and quantitative evaluation of p-mTOR, mTOR, p-AKT1 (Ser473), and AKT1 with or without PS210 (2 μM) by Western blotting in PASMCs under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + FLA-50 μg/ml group. (C–G) Primitive bands and quantitative densities of p-PI3K and PI3K by Western blots. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. 3% O 2 group. (H–N) BECC, ERI, and QCT treatment increased the stability of PI3K in PASMC protease lysates by the DARTS experiment. (H–K) Primitive Western blots of PI3K. (L–N) Quantitative evaluation of PI3K levels. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. DMSO group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-icc1.jpgAnti-AKT1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-16-1582677-g007.jpgAnti-AKT1 Monoclonal AntibodyFlavonoids inhibit the proliferation of PASMCs under hypoxic conditions by inhibiting the PI3K/AKT axis. (A, B) Primitive bands and quantitative densities of p-AKT1 Ser473 and AKT1 with or without Sc79 (20 μM) by Western blots in PASMCs under 3% O 2 . (C, D) Primitive bands and quantitative densities of p-PI3K, PI3K, p-AKT1 Ser473, and AKT1 with or without 740Y-P (10 μM) by Western blots in PASMCs under 3% O 2 . (E, F) Primitive bands and quantitative densities of p-PDPK1, PDPK, p-AKT1 Ser473, and AKT1 with or without MYH1485 (2 μM) in PASMCs by Western blots under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + Fla-50 μg/ml group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-icc2.jpgAnti-AKT1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-fphar-16-1582677-g004.jpgAnti-AKT1 Monoclonal AntibodyBECCs regulate the AKT/GSK3β/CDK/cyclin signaling pathway in HAPH rats. (A–C) Primitive bands of p-AKT1 S473, AKT1, p-GSK3β, GSK3β, CDK4, cyclin D1, CDK2, cyclin A, and P27 by Western blots in lung tissues. (D–F) Quantitative evaluation of p-AKT1 (S473), AKT1, p-GSK3β, GSK3β, CDK4, cyclin D1, CDK2, cyclin A, and P27 in the lung tissues. n = 5. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. hypoxia group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-icc3.jpgAnti-AKT1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-icc4.jpgAnti-AKT1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-ip7.jpgAnti-AKT1 Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-akt1-primary-antibodies-wb-testing-3.jpg.pngAnti-AKT1 Monoclonal AntibodyWestern blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Control group-mouse hippocampus tissue, <br>
Lane 2: Model group-mouse hippocampus tissue, <br>
Lane 3: Drug treatment (0.1 g/kg) – Mouse hippocampus tissue，<br>
Lane 4: Drug treatment (0.3 g/kg) – Mouse hippocampus tissue, <br>
Lane 5: Drug treatment (0.6 g/kg) – Mouse hippocampus tissue, <br>
Lane 5: Drug treatment (1.2 g/kg) – Mouse hippocampus tissue.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monolonal antibody (A04887-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with ChemiDoc MP system. A specific band was detected for AKT1 at approximately 60 kDa. The expected band size for AKT1 is at 56 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-akt1-primary-antibodies-wb-testing-4.pngAnti-AKT1 Monoclonal AntibodyWestern blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Normal group-Rat colon tissue lysates, <br>
Lane 2: Control group-Rat colon tissue lysates, <br>
Lane 3: Drug treatment (low) –Rat colon tissue lysates，<br>
Lane 4: Drug treatment (medium) –Rat colon tissue lysates, <br>
Lane 5: Drug treatment (high) –Rat colon tissue lysates, <br>
Lane 5: Drug treatment (positive) –Rat colon tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monolonal antibody (A04887-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with ChemiDoc MP system. A specific band was detected for AKT1 at approximately 60 kDa. The expected band size for AKT1 is at 56 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-1-akt1-primary-antibodies-wb-review-2_1.pngAnti-AKT1 Monoclonal Antibody Western blot analysis of AKT1 using anti-AKT1 antibody (M00024-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: normal group-Rat skeletal muscle tissue lysates, <br>
Lane 2: model group-Rat skeletal muscle tissue lysates, <br>
Lane 3: high-dose group-Rat skeletal muscle tissue lysates, <br>
Lane 4: medium-dose group-Rat skeletal muscle tissue lysates, <br>
Lane 5: low-dose group-Rat skeletal muscle tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 56-60 kDa. The expected band size for AKT1 is at 56 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apolipoprotein-e-antibody-m00015-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00015-apoe-primary-antibodies-wb-testing-1.jpgAnti-Apolipoprotein E APOE Monoclonal Antibody Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (M00015). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified monoclonal antibody (Catalog # M00015) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00015-APOE-IHC-test-2.jpgAnti-Apolipoprotein E APOE Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human kidney, using Apolipoprotein E Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acetyl-coa-carboxylase-antibody-m01802-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01802-wb7.jpgAnti-Acetyl-CoA Carboxylase Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01802-ihc.jpgAnti-Acetyl-CoA Carboxylase Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human kidney, using Acetyl-CoA Carboxylase Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01802-wb8.jpgAnti-Acetyl-CoA Carboxylase Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01802-wb.jpgAnti-Acetyl-CoA Carboxylase Monoclonal AntibodyWestern blot analysis of Acetyl-CoA Carboxylase expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apc-antibody-m00008-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00008-apc-primary-antibodies-wb-testing-1.jpgAnti-APC Monoclonal Antibody Western blot analysis of APC using anti-APC antibody (M00008). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC antigen affinity purified monoclonal antibody (M00008) at a dilution of 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for APC at approximately 160 kDa. The expected band size for APC is at 312 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00008-apc-primary-antibodies-ihc-testing-2.jpgAnti-APC Monoclonal Antibody IHC analysis of APC using anti-APC antibody (M00008). <br>APC was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-APC Antibody (M00008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00008-apc-primary-antibodies-ihc-testing-3.jpgAnti-APC Monoclonal Antibody IHC analysis of APC using anti-APC antibody (M00008). <br>APC was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-APC Antibody (M00008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nalp3-nlrp3-rabbit-monoclonal-antibody-m00034-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-wb8.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-wb.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyWestern blot analysis of NLRP3 expression in SH-SY5Y cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-wb9.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-ihc.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human lung carcinoma, using NALP3 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-wb10.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-if.jpgAnti-NALP3 NLRP3 Rabbit Monoclonal AntibodyImmunofluorescent analysis of Raw264.7 cells, using NALP3 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-msk1-s376-antibody-mp04922-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp04922-wb.jpgAnti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal AntibodyWestern blot analysis of Phospho-MSK1 (S376) expression in HEK293 cell lysate treated with EGF.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp04922-ihc.jpgAnti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human lung cancer, using Phospho-MSK1 (S376) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-smc1-s957-antibody-mp02148-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02148-wb.jpgAnti-Phospho-SMC1 (S957) Monoclonal AntibodyWestern blot analysis of Phospho-SMC1 (S957) expression in (1) HeLa cell lysate; (2) Hela cells lysate treated with AP.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp02148-ihc.jpgAnti-Phospho-SMC1 (S957) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-SMC1 (S957) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myd88-antibody-m00025-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00025-myd88-primary-antibodies-wb-testing-1.jpgAnti-MyD88 Monoclonal AntibodyWestern blot analysis of MYD88 using anti-MYD88 antibody (M00025). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified monoclonal antibody (M00025) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 36 kDa. The expected band size for MYD88 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00025-ihc.jpgAnti-MyD88 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human breast cancer, using MyD88 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00025-if.jpgAnti-MyD88 Monoclonal AntibodyImmunofluorescent analysis of A549 cells, using MyD88 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-cdk2-y15-antibody-mp00166-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00166-p-cdk2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-CDK2 (Y15) Rabbit Monoclonal AntibodyWestern blot analysis of P-CDK2 using anti-P-CDK2 antibody (MP00166). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-CDK2 antigen affinity purified monoclonal antibody (MP00166) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-CDK2 at approximately 34 kDa. The expected band size for P-CDK2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00166-ihc.jpgAnti-Phospho-CDK2 (Y15) Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-CDK2 (Y15) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-runx2-antibody-m00442-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-runx2-primary-antibodies-wb-testing-1.jpgAnti-RUNX2 Monoclonal Antibody Western blot analysis of RUNX2 using anti-RUNX2 antibody (M00442). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified monoclonal antibody (Catalog # M00442) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 57 kDa. The expected band size for RUNX2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-if.jpgAnti-RUNX2 Monoclonal AntibodyImmunofluorescent analysis of Saos-2 cells, using RUNX2 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-runx2-primary-antibodies-ihc-testing-2.jpgAnti-RUNX2 Monoclonal Antibody IHC analysis of RUNX2 using anti-RUNX2 antibody (M00442). <br>RUNX2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:100 rabbit anti-RUNX2 Antibody (M00442) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-jak2-y1007-y1008-antibody-mp00027-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00027-wb.jpgAnti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal AntibodyWestern blot analysis of JAK2 phosphorylation expression in Jurkat cell lysates treated with Pervanadate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00027-ott-12-2011fig7.jpgAnti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal AntibodyEffect of curcumin on p-JAK2/p-STAT3 signaling in MG-63 cells. Notes: MG-63 cells were treated with curcumin (0, 5, 10, or 20 µM) for 24 hours. Cell lysates were harvested, and expression of p-JAK2, JAK2, p-STAT3, and STAT3 was determined by Western blot. GAPDH was used as an internal control. ** P <0.01 and *** P <0.001. Abbreviation: p, phosphorylated.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421868/'>30936718</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00027-ihc.jpgAnti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human cervix cancer, using Phospho-JAK2 (Y1007 + Y1008) Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00027-if.jpgAnti-Phospho-JAK2 (Y1007 + Y1008) Monoclonal AntibodyImmunofluorescent analysis of Jurkat cells treated with Pervanadate, using Phospho-JAK2 (Y1007 + Y1008) Antibody
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-histone-h2a-x-s139-antibody-mp00241-boster.html2026-03-24T05:19:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00241-histoneh2a.x-primary-antibodies-wb-testing-1.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody Western blot analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human U20S whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HistoneH2A.X antigen affinity purified monoclonal antibody (Catalog # MP00241) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HistoneH2A.X at approximately 15 kDa. The expected band size for HistoneH2A.X is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00241-histoneh2a.x-primary-antibodies-ihc-testing-2.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). <br>
HistoneH2A.X was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00241-histoneh2a.x-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). <br>
HistoneH2A.X was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00241-histoneh2a.x-primary-antibodies-ihc-testing-4.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). <br>
HistoneH2A.X was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp00241-histoneh2a.x-primary-antibodies-ihc-testing-5.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody IHC analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). <br>
HistoneH2A.X was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HistoneH2A.X Antibody (MP00241) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/P/MP00241-H2AFX-IF-test-3.jpgAnti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal AntibodyImmunofluorescent analysis of HeLa cells treated with H2O2, using Phospho-Histone H2A.X (S139) Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il10-antibody-m00021-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00021-il10-primary-antibodies-wb-testing-1.jpgAnti-IL10 Monoclonal Antibody Western blot analysis of IL10 using anti-IL10 antibody (M00021). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: rat spleen tissue lysates, <br>
Lane 4: rat PC-12 whole cell lysates, <br>
Lane 5: mouse spleen tissue lysates, <br>
Lane 6: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified monoclonal antibody (M00021) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa. The expected band size for IL10 is at 21 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-granzyme-b-antibody-m00353-1-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00353-1-ihc.jpgAnti-Granzyme B GZMB Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human T cell lymphoma, using Granzyme B Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-periostin-antibody-m01378-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01378-postn-primary-antibodies-wb-testing-1.jpgAnti-Periostin Monoclonal AntibodyWestern blot analysis of POSTN using anti-POSTN antibody (M01378). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human U251 whole cell lysates,<br>
Lane 5: rat liver tissue lysates,<br>
Lane 6: rat heart tissue lysates,<br>
Lane 7: mouse liver tissue lysates,<br>
Lane 8: mouse heart tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POSTN antigen affinity purified monoclonal antibody (M01378) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POSTN at approximately 90 kDa. The expected band size for POSTN is at 93 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-birc2-antibody-m01700-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01700-birc2-primary-antibodies-wb-testing-1.jpgAnti-BIRC2 Monoclonal AntibodyWestern blot analysis of BIRC2 using anti-BIRC2 antibody (M01700). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC2 antigen affinity purified monoclonal antibody (M01700) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BIRC2 at approximately 70 kDa. The expected band size for BIRC2 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01700-birc2-primary-antibodies-ihc-testing-1.jpgAnti-BIRC2 Monoclonal AntibodyIHC analysis of BIRC2 using anti-BIRC2 antibody (M01700). <br>BIRC2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BIRC2 Antibody (M01700) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01700-birc2-primary-antibodies-ihc-testing-2.jpgAnti-BIRC2 Monoclonal AntibodyIHC analysis of BIRC2 using anti-BIRC2 antibody (M01700). <br>BIRC2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BIRC2 Antibody (M01700) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irak4-antibody-m01247-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01247-wb.jpgAnti-IRAK4 Monoclonal AntibodyWestern blot analysis of IRAK4 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01247-icc1.jpgAnti-IRAK4 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01247-icc2.jpgAnti-IRAK4 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irak-antibody-m01021-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01021-wb.jpgAnti-IRAK IRAK1 Monoclonal AntibodyWestern blot analysis of IRAK expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glypican-3-antibody-m01922-1-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01922-1-gpc3-primary-antibodies-wb-testing-1.jpgAnti-Glypican 3 Monoclonal AntibodyWestern blot analysis of GPC3 using anti-GPC3 antibody (M01922-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPC3 antigen affinity purified monoclonal antibody (M01922-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPC3 at approximately 66 kDa. The expected band size for GPC3 is at 66 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lmo2-antibody-m03502-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03502-lmo2-primary-antibodies-wb-testing-1.jpgAnti-LMO2 Monoclonal Antibody Western blot analysis of LMO2 using anti-LMO2 antibody (M03502). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates,<br>
Lane 2: rat C6 whole cell lysates,<br>
Lane 3: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified monoclonal antibody (Catalog # M03502) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 19 kDa. The expected band size for LMO2 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03502-lmo2-primary-antibodies-ihc-testing-2.jpgAnti-LMO2 Monoclonal Antibody IHC analysis of LMO2 using anti-LMO2 antibody (M03502). <br>
LMO2 was detected in a paraffin-embedded section of lymph node of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-LMO2 Antibody (M03502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03502-lmo2-primary-antibodies-ihc-testing-3.jpgAnti-LMO2 Monoclonal Antibody IHC analysis of LMO2 using anti-LMO2 antibody (M03502). <br>
LMO2 was detected in a paraffin-embedded section of lymph node of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-LMO2 Antibody (M03502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03502-lmo2-primary-antibodies-ihc-testing-4.jpgAnti-LMO2 Monoclonal Antibody IHC analysis of LMO2 using anti-LMO2 antibody (M03502). <br>
LMO2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-LMO2 Antibody (M03502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03502-lmo2-primary-antibodies-ihc-testing-5.jpgAnti-LMO2 Monoclonal Antibody IHC analysis of LMO2 using anti-LMO2 antibody (M03502). <br>
LMO2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-LMO2 Antibody (M03502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wasl-antibody-m05438-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05438-wasl-primary-antibodies-wb-testing-1.jpgAnti-WASL Monoclonal AntibodyWestern blot analysis of WASL using anti-WASL antibody (M05438). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human RT4 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASL antigen affinity purified monoclonal antibody (M05438) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WASL at approximately 70 kDa. The expected band size for WASL is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05438-wasl-primary-antibodies-ihc-testing-1.jpgAnti-WASL Monoclonal AntibodyIHC analysis of WASL using anti-WASL antibody (M05438). <br>WASL was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WASL Antibody (M05438) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05438-wasl-primary-antibodies-ihc-testing-2.jpgAnti-WASL Monoclonal AntibodyIHC analysis of WASL using anti-WASL antibody (M05438). <br>WASL was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WASL Antibody (M05438) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05438-wasl-primary-antibodies-ihc-testing-3.jpgAnti-WASL Monoclonal AntibodyIHC analysis of WASL using anti-WASL antibody (M05438). <br>WASL was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WASL Antibody (M05438) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptger2-antibody-m04963-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04963-ptger2-primary-antibodies-wb-testing-1.jpgAnti-PTGER2 Monoclonal Antibody Western blot analysis of PTGER2 using anti-PTGER2 antibody (M04963). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human A431 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates,<br>
Lane 5: rat spleen tissue lysates,<br>
Lane 6: mouse lung tissue lysates,<br>
Lane 7: mouse spleen tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER2 antigen affinity purified monoclonal antibody (M04963) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTGER2 at approximately 52 kDa. The expected band size for PTGER2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04963-ptger2-primary-antibodies-ihc-testing-2.jpgAnti-PTGER2 Monoclonal Antibody IHC analysis of KRT13 using anti-KRT13 antibody (M04299). <br>KRT13 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KRT13 Antibody (M04299) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erab-antibody-m03844-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03844-wb.jpgAnti-ERAB Monoclonal AntibodyWestern blot analysis of ERAB expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mta2-antibody-m03073-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03073-mta2-primary-antibodies-wb-testing-1.jpgAnti-MTA2 Monoclonal Antibody Western blot analysis of MTA2 using anti-MTA2 antibody (M03073). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human RT4 whole cell lysates,<br>
Lane 3: human HEL whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: human K562 whole cell lysates,<br>
Lane 6: human A431 whole cell lysates,<br>
Lane 7: human PC-3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # M03073) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA2 at approximately 75 kDa. The expected band size for MTA2 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03073-mta2-primary-antibodies-ihc-testing-2.jpgAnti-MTA2 Monoclonal Antibody IHC analysis of MTA2 using anti-MTA2 antibody (M03073). <br>
MTA2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MTA2 Antibody (M03073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03073-mta2-primary-antibodies-ihc-testing-3.jpgAnti-MTA2 Monoclonal Antibody IHC analysis of MTA2 using anti-MTA2 antibody (M03073). <br>
MTA2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MTA2 Antibody (M03073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03073-mta2-primary-antibodies-ihc-testing-4.jpgAnti-MTA2 Monoclonal Antibody IHC analysis of MTA2 using anti-MTA2 antibody (M03073). <br>
MTA2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MTA2 Antibody (M03073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03073-mta2-primary-antibodies-ihc-testing-5.jpgAnti-MTA2 Monoclonal Antibody IHC analysis of MTA2 using anti-MTA2 antibody (M03073). <br>
MTA2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MTA2 Antibody (M03073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdk1-antibody-m01268-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01268-pdk1-primary-antibodies-wb-testing-1.jpgAnti-PDK1 Monoclonal AntibodyWestern blot analysis of PDK1 using anti-PDK1 antibody (M01268). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates,<br>
Lane 2: rat liver tissue lysates,<br>
Lane 3: mouse heart tissue lysates,<br>
Lane 4: mouse liver tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified monoclonal antibody (M01268) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 46 kDa. The expected band size for PDK1 is at 49 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-frs2-antibody-m02798-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02798-wb.jpgAnti-FRS2 Monoclonal AntibodyWestern blot analysis of FRS2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nrcam-antibody-m03746-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03746-nrcam-primary-antibodies-wb-testing-1.jpgAnti-NRCAM Monoclonal AntibodyWestern blot analysis of NRCAM using anti-NRCAM antibody (M03746). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRCAM antigen affinity purified monoclonal antibody (M03746) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRCAM at approximately 144 kDa. The expected band size for NRCAM is at 144 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbpl1-antibody-m07468-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07468-wb.jpgAnti-TBPL1 Monoclonal AntibodyWestern blot analysis of TBPL1 expression in (1) HeLa cell lysate; (1) Mouse testis lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07468-41598_2025_11634_fig1_html.pngAnti-TBPL1 Monoclonal AntibodyTBP and TBPL1 genes, but not TBPL2 , are expressed in healthy and breast cancer cell lines. Total RNA was isolated with Trizol and end-point RT-PCR was performed with the oligonucleotides shown in Table . ( A ) Electrophoresis on 1% bleach agarose gel showing the integrity of the RNA28S and RNA18S ribosomal genes in MCF-12F and MCF-10A healthy cell lines (lanes1 and 2, respectively), and MCF-7, T47D, SK-BR-3, and MDA-MB-231 breast cancer cell lines (lanes 3 to 7, respectively). ( B ) Amplification of a 232 bp DNA fragment corresponding to the TBP gene in all breast cell lines. ( C ) Amplification of a 289 bp DNA fragment corresponding to the TBPL1 gene in all cell lines. ( D ) TBPL2 gene was not expressed in any of the healthy cell lines or breast cancer cell lines. ( E ) As an internal control, a 298 bp DNA fragment corresponding to the B2M gene was amplified in all the breast cell lines studied. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-11634-2'>41152284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07468-41598_2025_11634_fig3_html.pngAnti-TBPL1 Monoclonal AntibodyIntracellular localization of TBPL1 protein by immunofluorescence and confocal microscopy in healthy and breast cancer cell lines. Cells were fixed and incubated with antibodies against TBPL1 (1:200 dilution) and stained with Rhodamine-Phalloidin (1:100 dilution) for actin cytoskeleton and with Hoechst 33,342 for the nuclei. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-11634-2'>41152284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07468-41598_2025_11634_fig4_html.pngAnti-TBPL1 Monoclonal AntibodyDetermination of the TBPL1 gene expression levels in untransfected and transfected healthy and breast cancer cell lines with the U6-gRNA Cas-92A + GFP-RM CRISPR plasmid. The TBPL1 gene mRNA levels were measured by end-point RT-PCR in all cell lines. As a control, the B2M mRNA expression level was also determined in all samples. RNA samples of the three independent experimental replicates for nontransfected and transfected cell lines were used as templates for RT-PCR assays before sending them for RNAseq analyses. ( A , C , E , G ) MCF-12, T47D, SK-BR-3, and MDA-MB-2321 nontransfected (NT) cell lines. ( B , D , F , H ) MCF-12, T47D, SK-BR-3, and MDA-MB-231 transfected (T) cell lines. ( I ) RNA gel electrophoresis shows the integrity of RNA18S and RNA28S ribosomal genes for all healthy and breast cancer cell lines. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-11634-2'>41152284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07468-41598_2025_11634_fig5_html.pngAnti-TBPL1 Monoclonal AntibodyDetermination of the TBPL1 protein expression level by Western blot. Protein extracts from nontransfected and transfected cell lines with the U6-gRNA Cas-92A + GFP-RM CRISPR plasmid were analyzed by Western blot. Membranes were probed with anti-TBPL1 and anti-Tubulin antibodies and revealed for chemoluminescence as described in Methods. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-11634-2'>41152284</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-citrate-synthetase-antibody-m05128-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05128-wb.jpgAnti-Citrate synthetase CS Monoclonal AntibodyWestern blot analysis of Citrate synthetase expression in (1) HeLa cell lysate; (1) Mouse spleen lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05128-41467_2025_57767_fig2_html.pngAnti-Citrate synthetase CS Monoclonal AntibodyEvaluation of the live-cell labeling by genetically encoded tyrosinases. a Live-cell protein labeling with genetically encoded Bacillus megaterium tyrosinase (BmTYR). HEK293T cells were transfected with NES-BmTYR for 24 h, treated with CuCl 2 at various concentrations for 1 h, followed by the treatment of 50 μM AP for 30 min. The cells were lysed and subjected to click-based in-gel fluorescence detection. V5 indicates the expression of BmTYR. b Confocal fluorescence imaging of mito-BmTYR and its impact on mitochondrial morphology. HEK293T cells were transfected with mito-BmTYR for 24 h, treated with 5 μM CuCl 2 for 1 h, followed by the treatment of 50 μM AP for 30 min. The cells were fixed and click with azide-biotin after cell fixation to visualize the labeled proteins. Streptavidin-AF647 indicates biotin labeling, and Citrate Synthase indicates the mitochondria. Scale bars, 10 μm. c The impact of CuCl 2 treatment and BmTYR labeling on cell viability. HEK293T cells were transfected with vehicle or NES-BmTYR for 24 h, followed by the treatment of 5 μM CuCl 2 for 1 h and AP labeling for 30 min. The cell viability was determined by the MTS assay. Quantification was performed from three biological replicates and the error bars show mean ± SD. *** p < 0.001 (one-sided student t-test). P -values: 0.00084 (5 μM CuCl 2 ), 0.01284 (50 μM AP), 0.43725 (pcDNA3.1-BmTYR-NES), 0.00092 (pcDNA3.1-BmTYR-NES + 5 μM CuCl 2 ), 0.00005 (pcDNA3.1-BmTYR-NES + 5 μM CuCl 2 + 50 μM AP). d The schematic of superTOP-ABPP to identify modification sites by mito-BmTYR and mito-APEX2 labeling. HEK293T cells were transfected with mito-BmTYR or mito-APEX2 for 24 h and subjected to the corresponding labeling. After digesting the cell lysates into peptides, the labeled peptides were enriched by click reaction with azide-functionalized acid-cleavable agarose beads. The enriched peptides were released by acid cleavage and identified by LC-MS/MS analysis. e The number of cysteines, lysines and histidines labeled by mito-BmTYR with phenol or quinone type of modifications. Subcellular compartment analysis of proteins labeled by mito-BmTYR ( f ) or mito-APEX2 ( g ) with AP. The subcellular information is based on Uniport annotations. Three replicates were performed with similar results. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-57767-w'>40089463</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdt1-antibody-m01035-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01035-cdt1-primary-antibodies-wb-testing-1.jpgAnti-CDT1 Rabbit Monoclonal AntibodyWestern blot analysis of CDT1 using anti-CDT1 antibody (M01035). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1 antigen affinity purified monoclonal antibody (M01035) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDT1 at approximately 70 kDa. The expected band size for CDT1 is at 65 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcn2-antibody-m01172-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01172-wb.jpgAnti-GCN2 EIF2AK4 Rabbit Monoclonal AntibodyWestern blot analysis of GCN2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itk-antibody-m01385-boster.html2026-03-24T05:19:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01385-wb.jpgAnti-ITK Monoclonal AntibodyWestern blot analysis of ITK expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-staufen-antibody-m04259-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04259-stau1-primary-antibodies-wb-testing-1.jpgAnti-Staufen STAU1 Monoclonal AntibodyWestern blot analysis of STAU1 using anti-STAU1 antibody (M04259). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAU1 antigen affinity purified monoclonal antibody (M04259) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAU1 at approximately 55 kDa. The expected band size for STAU1 is at 63 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-golgin-97-antibody-m13524-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13524-wb.jpgAnti-Golgin 97 GOLGA1 Monoclonal AntibodyWestern blot analysis of Golgin 97 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdcd4-antibody-m01105-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01105-wb7.jpgAnti-PDCD4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01105-fcell-09-643525-g006.jpgAnti-PDCD4 Rabbit Monoclonal AntibodyAndrographolide (Andro) inhibits breast cancer cell proliferation, migration, and invasion via NF-κB/miR-21-5p/PDCD4 signaling. (A) Heat map of the qRT-PCR data of miRNA expression in MCF-7 cells with or without NF-κB inhibition. APDC: NF-κB inhibitor. (B) miR-21-5p expression in breast cancer tissue and MCF cells was also detected using qRT-PCR. miR-21-5p was markedly suppressed by Andro in MCF-7 cells and MMTV-PyMT mice. (C) Colony formation, wound healing, Transwell migration, and Matrigel invasion assay. Immunohistochemistry (D) and Immunoblotting assay (E) for PDCD4 expression in DMSO- and Andro-treated MMTV-PyMT mice. (F) Immunoblotting analysis for PDCD4 expression in MCF-7 cells. (G) Schematic of the mechanisms of Andro-mediated effect on luminal-like breast cancer growth and metastasis. Quantitative data are expressed as the mean ± SD, n = 3. Significant effect: * P < 0.05, ** P < 0.01, *** P < 0.005. ns , no significant effect. Scale bar , 100 μM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.643525/full'>34249905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01105-wb.jpgAnti-PDCD4 Rabbit Monoclonal AntibodyWestern blot analysis of PDCD4 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zap70-antibody-m00754-3-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-3-wb.jpgAnti-ZAP70 Monoclonal AntibodyWestern blot analysis of ZAP70 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pon1-antibody-m00516-3-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-3-wb.jpgAnti-PON1 Monoclonal AntibodyWestern blot analysis of PON1 expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p23-antibody-m04136-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04136-wb.jpgAnti-p23 Monoclonal AntibodyWestern blot analysis of p23 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC12 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd1-antibody-m02336-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02336-mbd1-primary-antibodies-wb-testing-1.jpgAnti-MBD1 Monoclonal Antibody Western blot analysis of MBD1 using anti-MBD1 antibody (M02336). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD1 antigen affinity purified monoclonal antibody (Catalog # M02336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD1 at approximately 67, 80 kDa. The expected band size for MBD1 is at 67 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bag3-antibody-m01547-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01547-wb.jpgAnti-Bag3 Monoclonal AntibodyWestern blot analysis of Bag3 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-menin-antibody-m00331-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00331-men1-primary-antibodies-wb-testing-1.jpgAnti-Menin MEN1 Monoclonal Antibody Western blot analysis of Menin using anti-Menin antibody (M00331). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HEL whole cell lysates, <br>
Lane 4: human MCF-7 whole cell lysates, <br>
Lane 5: human Hacat whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human SH-SY5Y whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Menin antigen affinity purified monoclonal antibody (M00331) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Menin at approximately 72 kDa. The expected band size for Menin is at 67 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nf2-merlin-antibody-m00279-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00279-nf2-primary-antibodies-wb-testing-1.jpgAnti-NF2 / Merlin Monoclonal AntibodyWestern blot analysis of NF2 using anti-NF2 antibody (M00279). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human THP-1 whole cell lysates,<br>
Lane 4: rat PC-12 whole cell lysates,<br>
Lane 5: mouse kidney tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2 antigen affinity purified monoclonal antibody (M00279) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF2 at approximately 70 kDa. The expected band size for NF2 is at 70 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ceacam6-antibody-m03197-1-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03197-1-ceacam6-primary-antibodies-wb-testing-1.jpgAnti-CEACAM6 Rabbit Monoclonal AntibodyWestern blot analysis of CEACAM6 using anti-CEACAM6 antibody (M03197-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM6 antigen affinity purified monoclonal antibody (M03197-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CEACAM6 at approximately 60 kDa. The expected band size for CEACAM6 is at 37 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prothrombin-antibody-m00044-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00044-wb.jpgAnti-Prothrombin F2 Monoclonal AntibodyWestern blot analysis of Prothrombin expression in human serum lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dhfr-antibody-m00813-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-wb.jpgAnti-DHFR Monoclonal AntibodyWestern blot analysis of DHFR expression in (1) HeLa cell lysate; (2) Mouse spleen lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-wb9.jpgAnti-DHFR Monoclonal AntibodyAll lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-wb7.jpgAnti-DHFR Monoclonal AntibodyAll lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-wb8.jpgAnti-DHFR Monoclonal AntibodyAll lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dazl-antibody-m02069-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02069-wb.jpgAnti-DAZL Monoclonal AntibodyWestern blot analysis of DAZL expression in human testis lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ferritin-light-chain-antibody-m01956-1-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-ftl-primary-antibodies-wb-testing-1.jpgAnti-Ferritin Light Chain FTL Monoclonal Antibody Western blot analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human THP-1 whole cell lysates,<br>
Lane 4: human SiHa whole cell lysates,<br>
Lane 5: rat liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ferritin Light Chain antigen affinity purified monoclonal antibody (Catalog # M01956-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ferritin Light Chain at approximately 20 kDa. The expected band size for Ferritin Light Chain is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-wb.jpgAnti-Ferritin Light Chain FTL Monoclonal AntibodyWestern blot analysis of Ferritin Light Chain expression in (1) HepG2 cell lysate; (2) Mouse liver lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-ftl-primary-antibodies-ihc-testing-2.jpgAnti-Ferritin Light Chain FTL Monoclonal Antibody IHC analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). <br>
Ferritin Light Chain was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ferritin Light Chain Antibody (M01956-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-ftl-primary-antibodies-ihc-testing-3.jpgAnti-Ferritin Light Chain FTL Monoclonal Antibody IHC analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). <br>
Ferritin Light Chain was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ferritin Light Chain Antibody (M01956-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-ftl-primary-antibodies-ihc-testing-4.jpgAnti-Ferritin Light Chain FTL Monoclonal Antibody IHC analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). <br>
Ferritin Light Chain was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ferritin Light Chain Antibody (M01956-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01956-1-ftl-primary-antibodies-ihc-testing-5.jpgAnti-Ferritin Light Chain FTL Monoclonal Antibody IHC analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). <br>
Ferritin Light Chain was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ferritin Light Chain Antibody (M01956-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itch-antibody-m00195-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00195-wb.jpgAnti-ITCH Monoclonal AntibodyWestern blot analysis of ITCH expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hec1-antibody-m01731-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01731-wb.jpgAnti-HEC1 NDC80 Monoclonal AntibodyWestern blot analysis of HEC1 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpx4-antibody-m02059-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-gpx4-primary-antibodies-wb-testing-1_1.jpgAnti-GPX4 Rabbit Monoclonal Antibody Western blot analysis of GPX4 using anti-GPX4 antibody (M02059). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse RAW264.7(WT) whole cell lysates, <br>
Lane 2: mouse RAW264.7(KO) whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: rat PC-12 whole cell lysates, <br>
Lane 6: rat NRK whole cell lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates, <br>
Lane 8: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX4 antigen affinity purified monoclonal antibody (M02059) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_60159_fig4_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyImmunohistochemically stained pancreas tissue ( n = 6). Protein levels of ( A ) GPX4, ( B ) ACSL4, and ( C ) LPCAT3 in rats (×400). GPX4, glutathione peroxidase 4; xCT, cysteine/glutamate transporter; ACSL4, acyl-CoA synthetase long-chain family member 4. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-60159-7'>38664508</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-s2.0-s0147651325011418-gr2.jpgAnti-GPX4 Rabbit Monoclonal AntibodyVitamin C improved PM2.5-induced female rats’ thyroid ferroptosis. (A) Immunofluorescence assay was used to detect the expression of GPX4 protein in thyroid tissues. (B) Quantitative immunofluorescence analysis (n = 3/group). (C) Serum malondialdehyde (MDA) content. (D) Serum ferrous ion (Fe2+) content (n = 10/group). The data are presented as mean ± SD. Compared with the Con group, *P < 0.05 or ** P < 0.01; Compared with the Mod group, # P < 0.05, ## P < 0.01, or ### P < 0.001. Con: Control group, normal environment for eight weeks; Vc: vitamin C was administered by gavage at 120 mg/kg for eight weeks; Mod: PM2.5 exposure for eight weeks; Mod + Vc: after PM2.5 exposure, vitamin C was administered by gavage at 120 mg/kg for eight weeks.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325011418'>40753780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-s2.0-s0147651325011418-gr4.jpgAnti-GPX4 Rabbit Monoclonal AntibodyVitamin C regulated the protein expression levels of IL-17A signaling pathway and ferroptosis-related factors. (A) The expression levels of the IL-17A pathway (B, IL-17A; C, IL-17RA; D, ACT1) and ferroptosis (E, GPX4)-related genes in the thyroid tissues were determined by western blot (n = 3/group).The data are presented as mean ± SD. Compared with the Con group, *P < 0.05, **P < 0.01, or ***P < 0.001; Compared with the Mod group, # P < 0.05 or ## P < 0.01. Con: Control group, normal environment for eight weeks; Vc: vitamin C was administered by gavage at 120 mg/kg for eight weeks; Mod: PM2.5 exposure for eight weeks; Mod + Vc: after PM2.5 exposure, vitamin C was administered by gavage at 120 mg/kg for eight weeks.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325011418'>40753780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-s2.0-s0147651325011418-gr6.jpgAnti-GPX4 Rabbit Monoclonal AntibodyEffect of Vitamin C on FRTL5 cell function and IL-17A signaling pathway and ferroptosis-related factors stimulated by PM2.5. (A-C) The levels of (A, TSH; B, FT4; and C, FT3) in the supernatant of FRTL5 cells were detected by ELISA. (D-G) ELISA was used to measure the levels of IL-17A (D), IL-17RA (E), ACT1 (F), and GPX4 (G) in the supernatant of FRTL5 cells. The data are presented as mean ± SD; n = 5/group). Compared with the Con group, ** P < 0.01; Compared with the Mod group, # P < 0.05 or ## P < 0.01. Con: Control group, normal medium for 24 h; Vc: medium containing 50 μmol/L Vc for 24 h; Mod: medium containing 400 μg/mL PM2.5 for 24 h; Mod + Vc: medium containing 400 μg/mL PM2.5 and 50 μmol/L Vc for 24 h; Mod + Y320: medium containing 400 μg/mL PM2.5 and 0.08 μmol/L Y320 for 24 h.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325011418'>40753780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-s2.0-s0147651325011418-gr7.jpgAnti-GPX4 Rabbit Monoclonal AntibodyEffects of Vitamin C intervention on the IL-17A/IL-17RA/ACT1 signaling pathway and ferroptosis in FRTL5 cells stimulated by PM2.5. (A-D) qPCR was used to detect the transcription levels of IL-17A/IL-17RA/ACT1 signaling pathway-related factors. (E-I) The protein expression levels of the IL-17A/IL-17RA/ACT1 signaling pathway were detected by western blot. The data are presented as mean ± SD; n = 3/group. Compared with the Con group, * P < 0.05 or ** P < 0.01; Compared with the Mod group, # P < 0.05 or ## P < 0.01. Con: Control group, normal medium for 24 h; Vc: medium containing 50 μmol/L Vc for 24 h; Mod: medium containing 400 μg/mL PM2.5 for 24 h; Mod + Vc: medium containing 400 μg/mL PM2.5 and 50 μmol/L Vc for 24 h; Mod + Y320: medium containing 400 μg/mL PM2.5 and 0.08 μmol/L Y320 for 24 h.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325011418'>40753780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-icc1.jpgAnti-GPX4 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_60159_fig3_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyFerroptosis was observed and alleviated in the pancreas of rats ( n = 6). ( A ) Ultrastructure of the mitochondria in the pancreas obtained by TEM (×20,000); ( B – F ) Western blot analysis of ferroptosis-related proteins, GPX4, xCT, ACSL4, and LPCAT3. GPX4, glutathione peroxidase 4; xCT, cysteine/glutamate transporter; ACSL4, acyl-CoA synthetase long-chain family member 4; LPCAT3, lysophosphatidylcholine acyltransferase 3; C, control; AP, acute pancreatitis; HTG, hypertriglyceridemic; HTGP, HTG pancreatitis; *b vs. the C group, *c vs. the AP group, *B vs. the HTG group, *C vs. the HTGP group, * P < 0.05, # P < 0.01, • P < 0.001, ns: no significance. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-60159-7'>38664508</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-gpx4-primary-antibodies-wb-testing-2.jpgAnti-GPX4 Rabbit Monoclonal Antibody Western blot analysis of GPX4 using anti-GPX4 antibody (M02059). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat testis tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX4 antigen affinity purified monoclonal antibody (Catalog # M02059) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-gpx4-primary-antibodies-wb-testing-4.jpg.pngAnti-GPX4 Rabbit Monoclonal AntibodyWestern blot analysis of GPX4 using anti-GPX4 antibody (M02059). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1-3: model group-mouse uterine tissue lysates, <br>
Lane 4-6: young group-mouse uterine tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX4 antigen affinity purified monoclonal antibody (M02059) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 19 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_80160_fig7_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyEffects of GPX4 intervention blocking MT1G overexpression on GC iron autophagy and function. ( a ) Fluorescence expression of Fe 2+ in different GC cell lines Scale bar = 50 μm. ( b ) Observation of different GC cell lines under transmission electron microscopy. Scale bar = 2 μm .Scale bar = 500 nm. ( c ) Statistical analysis of 24-h cell migration in Transwell experimental GC cell line. ( d ) Transwell experiment GC cell line 24-h cell invasion statistics. ( e ) Microscopic observation of 24-h cell migration in Transwell experimental GC cell line. Scale bar = 50 μm. ( f ) Microscopic observation of 24-h cell migration in Transwell experiment GC cell line. Scale bar = 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-fonc-12-874900-g004.jpgAnti-GPX4 Rabbit Monoclonal AntibodySS attenuated the oxidation resistance of LUAD cells. (A, B) GSH or Cys was detected in Calu-1 or A549 cells, which were respectively treated with 10, 15, 20 μM or 20, 25, 30 μM SS for 6 h, and pretreated with or without Fer-1 (1 μM), the data statistic was shown in a histogram (*P < 0.05, **P < 0.01, ***P < 0.001). (C) Western blot analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 10, 15, 20 μM SS for 6 h. (D) Quantitative analysis of gray value of the SLC7A11 and GPX4 blots. (E) Western blotting analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 20 μM SS or 4 μM erastin, with or without Fer-1 (1 μM) for 6 h. (F) Quantitative analysis of gray value of the SLC7A11 and GPX4 blots.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9158126/&orig_host=www.ncbi.nlm.nih.gov'>35664792</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig6_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyPCTR1 activates CREB by the ALX/PKA pathway to increase GPX4 expression in vitro. BOC-2 (ALX receptor inhibitor, 10 μM), H89 (PKA inhibitor, 10 μM), 666-15 (CREB inhibitor, 1 μM) or an equivalent volume of DMSO was administered to H1299 cells for 30 min in advance, and then LPS and PCTR1 were co-administered for 48 h. A , B The protein level of P- PKA was measured by western blot. C , D The protein level of P-CREB was measured by western blot. E , F The protein level of GPX4 was measured by western blot. Data are presented as the mean ± SD, n = 5–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig3_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyPCTR1 activates CREB via the ALX/PKA pathway to increase GPX4 expression in vivo. PCTR1 at a dose of 200 ng was injected into each mouse via the caudal vein 6 h after LPS (15 mg/kg, ip) administration. BOC‐2 (ALX receptor inhibitor, 600 ng/kg), H89 (PKA inhibitor, 10 mg/kg), 666-15 (CREB inhibitor, 10 mg/kg) or an equivalent volume of DMSO was injected into the caudal vein 1 h before PCTR1 treatment. The mice were sacrificed 24 h after LPS stimulation. A , B The protein expression level of P-PKA was determined by western blotting. C , D The protein expression level of P-CREB was determined by western blotting. E , F The protein expression level of GPX4 was determined by western blotting. Data are presented as the mean ± SD, n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05. ip: intraperitoneal <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_80160_fig2_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyA correlation between the effect of MT1G on GC cell lines and the occurrence of ferroptosis. ( a ) MDA expression in different GC cell lines. ( b ) The expression of SOD in different GC cell line. ( c ) The expression of GSH in different GC cell lines. ( d ) The expression of ferroptosis related proteins in different GC cell lines. ( e ) The expression of GPX4 protein in different GC cell lines. ( f ) The expression of FSP1 protein in different GC cell lines. ( g ) Expression of SLC7A11 protein in different GC cell lines. ( h ) The expression of DHODH protein in different GC cell lines. ( i ) Fluorescence intensity of Fe 2+ content. Scale bar = 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-jcav16p1598g007.jpgAnti-GPX4 Rabbit Monoclonal AntibodyM2c macrophages increase ferroptosis resistance in gastric cancer cells. a The CCK-8 method was used to detect the survival of gastric cancer cells (Hgc27 and MKN45) intervened with RSL3 for 24 h. b The CCK-8 method was used to detect the survival of gastric cancer cells (Hgc27 and MKN45) intervened with Fer-1 for 24 h. c The expression of SOD in different intervention groups. d The expression of MDA in different intervention groups. e The expression of GSH in different intervention groups. f The expression of TGFβ1 protein WB in different cell lines. g The expression results of TGFβ1 protein. h The expression of key ferroptosis proteins WB in different cell lines. i The expression results of FSP1 protein. j Expression results of DHODH protein. k Expression results of GPX4 protein. l SLC7A11 protein expression results. m The intervention of RSL3 on the expression of key ferroptosis protein WB in different co culture groups. n The expression results of GPX4 protein. o SLC7A11 protein expression results. p The WB expression of key proteins involved in ferroptosis in different intervention groups. q The expression results of GPX4 protein. r The expression results of SLC7A11 protein. s Fluorescence results of mitochondrial membrane potential in different intervention groups. Scale bar=50 μm. *p<0.05, **p<0.01, ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11843238/'>39991579</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-fphar-16-1531277-g007.jpgAnti-GPX4 Rabbit Monoclonal AntibodyLiver-specific GPX4 knockdown inhibits TA-regulated ER stress in APAP-induced hepatotoxicity. (A) Western blotting assessed hepatic GRP78 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (B) q-PCR was conducted to evaluate the transcript levels of the Grp78 gene. (C) Western blotting assessed hepatic phosphorylated-PERK, phosphorylated-eIF2α, PERK, and eIF2α protein expressions. Band intensities were quantified using ImageJ. (D) Western blotting assessed hepatic ATF4 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (E) q-PCR was conducted to evaluate the transcript levels of the Atf4 gene. All data were presented as mean ± SD, n = 3 for Western blotting, n = 5 for others. * P < 0.05 vs. corresponding control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1531277/full'>40051561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-fphar-16-1531277-g006.jpgAnti-GPX4 Rabbit Monoclonal AntibodyLiver-specific GPX4 knockdown abrogates the protective effect of TA against APAP-induced hepatocyte apoptosis. (A) Western blotting assessed hepatic BCL2 and BAX expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (B) q-PCR was conducted to evaluate the transcript levels of BCL2 and BAX-related genes. (C) Hepatic caspase 3 activity was quantified using a commercially available kit (fold of control). (D) Western blotting assessed hepatic CHOP expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (E) q-PCR was conducted to evaluate the transcript levels of the Chop gene. All data were presented as mean ± SD, n = 3 for Western blotting, n = 5 for others. * P < 0.05 vs. corresponding control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1531277/full'>40051561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-fphar-16-1531277-g005.jpgAnti-GPX4 Rabbit Monoclonal AntibodyLiver-specific GPX4 knockdown abrogates the hepatoprotective effects of TA against APAP-induced hepatotoxicity. (A) Western blotting assessed the detected hepatic GPX4 knockdown efficiency in protein levels. Hepatocyte-specific GPX4 knockdown mice were created by AAV8-mediated delivery of a TBG promoter-driven shRNA targeting GPX4. Null-vector-injected mice served as control. (B) Plasma levels of ALT and AST. (C) Liver tissues were subjected to H&E staining for histological examination. (D) Concentrations of hepatic IL-1β, IL-6, TNF-α, and MCP-1 were quantified using commercially available ELISA kits. All data were presented as mean ± SD, n = 3 for Western blotting, n = 5 for others. * P < 0.05 vs. corresponding control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1531277/full'>40051561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-fphar-16-1531277-g004.jpgAnti-GPX4 Rabbit Monoclonal AntibodyTA mediates the enhancement of hepatic antioxidant function by GPX4. (A) Hepatic concentrations of MDA, alongside the enzymatic activities of SOD and CAT. (B) Hepatic concentrations of GSH-Px, GSH, and the GSH redox ratio (GSH/GSSG) were determined. (C) Western blotting assessed hepatic GPX4 expression, with β-actin as a loading control. Band intensities were quantified using ImageJ. (D) q-PCR was conducted to evaluate the transcript levels of the Gpx4 gene. All data were presented as mean ± SD, n = 3 for Western blotting, n = 6–8 for others. * P < 0.05 vs. corresponding control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1531277/full'>40051561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig1_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyFerroptosis is induced in LPS-induced ALI and associated with lung damage. LPS (15 mg/kg) in saline or an equivalent volume of saline was intraperitoneally injected into mice, and lung tissues were collected at 0, 12, 24, and 48 h, respectively. A–C Representative western blotting and quantification analysis of GPX4 and PTGS2. D–F Relative values of Fe 2+ , GSH and MDA concentrations. Mice were pretreated with ferrostatin-1 (10 mg/kg, ip) 1h1 h before being injected with LPS (15 mg/kg, ip). Lung samples were collected 24 h after LPS injection. G Representative H&E staining of lung tissues (original magnification, ×200; inset, ×400). H Acute lung injury score of each group. I–K The relative mRNA expression levels of the inflammatory cytokines: IL-6, TNF-α and IL-1β. The acute lung injury score data are presented as the median and range (25th–75th percentile), and other data are presented as the mean ± SD. n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05. ip: intraperitoneal <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig2_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyEffects of PCTR1 on ferroptosis in LPS-induced ALI. PCTR1 (100 or 200 ng) was injected into the caudal vein of mice 6 h after LPS (15 mg/kg, ip) treatment. All lung specimens were harvested at 24 h after LPS stimulation. A–C Representative western blotting and quantification analysis of GPX4 and PTGS2. D–F Relative values of Fe 2+ , GSH and MDA concentrations. G Representative TEM images of each group. The black arrow indicates ferroptotic mitochondria. Magnification ×30,000. H Representative H&E staining of lung tissues (original magnification, ×200; inset, ×400). I Acute lung injury score. J–L The relative mRNA expression levels of the inflammatory cytokines: IL-6, TNF-α and IL-1β. The acute lung injury score data are presented as the median and range (25th–75th percentile), and other data are presented as the mean ± SD. n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05. ip: intraperitoneal <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_80160_fig4_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyThe correlation between MT1G overexpression induced ferroptosis and autophagy in GC cell lines. ( a ) Expression of autophagy related proteins in different GC cell lines. ( b ) The protein expression ratio of LC3B I and LC3B II in different GC cell lines. ( c ) The expression of GPX4 protein in different GC cell lines. ( d ) The expression of SQSTM1 protein in different GC cell lines. ( e ) Co-IP detection of the correlation between GPX4 and SQSTM1 in GC cell lines. ( f ) Observation of different GC cell lines under transmission electron microscopy. Scale bar = 2 μm .Scale bar = 500 nm. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig5_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyEffects of PCTR1 on LPS-induced ferroptosis in vitro. A H1299 cells were treated with LPS (10 μg/mL) and different concentrations of PCTR1 for 48 h. Fold change in cell viability. B H1299 cells were stimulated with RSL3 (10 nM) and different concentrations of PCTR1 for 48 h. Fold change in cell viability. C–E Representative western blotting and quantification analysis of GPX4 and PTGS2. F–H Relative levels of Fe 2+ , GSH and MDA. I The level of lipid peroxidation was determined with the C11-BODIPY 581/591 fluorescent probe (original magnification ×400). J Oxidized C11-BODIPY 581/591 probe was quantified by flow cytometry. Data are presented as the mean ± SD, n = 4–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_80160_fig5_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyMT1G overexpression in GC cell line intervenes in GPX4/SQSTM1 axis to initiate iron autophagy. ( a ) Comparison of protein associations between TCGA non cancerous tissue and gastric cancer tissue. ( b ) Correlation heatmap between MT1G and GPX4/SQSTM1 axis related proteins. ( c ) The expression status of GPX4/SQSTM1 axis related proteins in the high and low expression queue of gastric adenocarcinoma. ( d ) The expression of GPX4/SQSTM1 axis related proteins in different GC cell lines. ( e ) The ratio of GPX4 protein expression in different GC cell lines. ( f ) The expression of SQSTM1 protein in different GC cell lines. ( g ) The expression of ARNTL protein in different GC cell lines. ( h ) The expression of EGLN2 protein in different GC cell lines. ( i ) Co-IP detection of the correlation between SQSTM1 and ARNTL in GC cell lines. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-12967_2023_4111_fig7_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyCREB mediates the effect of PCTR1 on eliminating lipid peroxides in vitro. 666-15 (CREB inhibitor, 1 μM) or an equivalent volume of DMSO was administered to H1299 cells for 30 min in advance, and then LPS and PCTR1 were co-administered for 48 h. A Immunofluorescence staining images of GPX4 (original magnification ×400). B , C Relative expression levels of GSH, MDA and 4-HNE. D The level of lipid peroxidation was determined with the C11-BODIPY 581/591 fluorescent probe (original magnification ×400). E Oxidized C11-BODIPY 581/591 probe was quantified by flow cytometry. Data are presented as the mean ± SD, n = 5–6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns: p > 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04111-9'>37121999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_80160_fig6_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyIntervention of GPX4 blocks ferroptosis induced by MT1G overexpression. ( a ) The CCK-8 method was used to detect the survival of GC cells intervened with DFOM for 24 h. ( b ) The CCK-8 method was used to detect the survival of GC cells intervened with RSL3 for 24 h. ( c ) The CCK-8 method was used to detect the survival of GC cells intervened with Fer-1 for 24 h. ( d ) The effects of different ferroptosis inhibitors on the activity of GC cells. ( e ) RT-qPCR was used to examine the effects of small molecule compounds RSL3 and Fer-1 on MT1G transfection. ( f ) The effects of small molecule compounds RSL3 and Fer-1 on GPX4 protein expression in different GC cell lines. ( g ) Statistical analysis of GPX4 protein expression in different cell lines. ( h ) The effect of small molecule compound Fer-1 on the expression of GPX4/SQSTM1 axis protein. ( i ) The expression of GPX4 protein in different GC cell lines. ( j ) The expression of SQSTM1 protein in different GC cell lines. ( k ) The expression of ARNTL protein in different GC cell lines. ( l ) The expression of EGLN2 protein in different GC cell lines. ( m ) Fluorescence expression of different GC cell lines JC-1. Scale bar = 50 μm. ( n ) Fluorescence statistics of different GC cell lines JC-1. ( o ) MDA expression in different GC cell lines. ( p ) SOD expression in different GC cell lines. ( q ) The expression of GSH in different GC cell lines. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-41598_2024_60159_fig1_html.pngAnti-GPX4 Rabbit Monoclonal AntibodyThe procedure of rat model and treatment time ( n = 6). ( A ) Animal modeling group and ( B ) treatment schedule of rats. C, control; AP, acute pancreatitis group; Lip-1, liproxstatin-1; HTG, hypertriglyceridemia; AMY, amylase; TEM, transmission electron microscopy; ROS, reactive oxygen species; MDA, malondialdehyde; GSH, glutathione; GPX4, glutathione peroxidase 4; xCT, cysteine/glutamate transporter; ACSL4, acyl-CoA synthetase long-chain family member 4; LPCAT3, lysophosphatidylcholine acyltransferase. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-60159-7'>38664508</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-gpx4-antibodies-wb-testing-3.jpgAnti-GPX4 Rabbit Monoclonal Antibody Western blot analysis of GPX4 using anti-GPX4 antibody (M02059). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCT116- WT whole cell lysates, <br>
Lane 2: human HCT116-GPX4 KO whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-GPX4 antigen affinity purified monoclonal antibody (M02059) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-serpinc1-antibody-m00469-boster.html2026-03-24T05:19:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00469-wb.jpgAnti-SERPINC1 Monoclonal AntibodyWestern blot analysis of SERPINC1 expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sphk1-antibody-m01390-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01390-wb.jpgAnti-SPHK1 Monoclonal AntibodyWestern blot analysis of SPHK1 expression in Raji cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01390-wb7.jpgAnti-SPHK1 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01390-wb8.jpgAnti-SPHK1 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-orm1-antibody-m04684-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04684-wb.jpgAnti-ORM1 Monoclonal AntibodyWestern blot analysis of ORM1 expression in human testis lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bst2-antibody-m00934-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00934-wb.jpgAnti-BST2 Monoclonal AntibodyWestern blot analysis of BST2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00934-bst2-primary-antibodies-ihc-testing-1.jpgAnti-BST2 Monoclonal AntibodyIHC analysis of BST2 using anti-BST2 antibody (M00934). <br>BST2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BST2 Antibody (M00934) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-txnrd1-antibody-m01778-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01778-txnrd1-primary-antibodies-wb-testing-1.jpgAnti-TXNRD1 Rabbit Monoclonal Antibody Western blot analysis of TXNRD1 using anti-TXNRD1 antibody (M01778). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antigen affinity purified monoclonal antibody (Catalog # M01778) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNRD1 at approximately 55 kDa. The expected band size for TXNRD1 is at 71 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgb2-antibody-m02651-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02651-wb.jpgAnti-HMGB2 Rabbit Monoclonal AntibodyWestern blot analysis of HMGB2 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad50-antibody-m00347-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00347-icc1.jpgAnti-Rad50 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00347-wb.jpgAnti-Rad50 Monoclonal AntibodyWestern blot analysis of Rad50 expression in Jurkat cell lysate;
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clock-antibody-m00064-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00064-wb.jpgAnti-CLOCK Monoclonal AntibodyWestern blot analysis of CLOCK expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00064-ihc.jpgAnti-CLOCK Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using CLOCK Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00064-if.jpgAnti-CLOCK Monoclonal AntibodyImmunofluorescent analysis of HeLa cells, using CLOCK Antibody .
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-factor-h-antibody-m00562-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00562-cfh-primary-antibodies-wb-testing-1.jpgAnti-Factor H CFH Monoclonal AntibodyWestern blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (M00562). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified monoclonal antibody (M00562) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 180 kDa. The expected band size for Factor H/CFH is at 139 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-meis1-antibody-m01602-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01602-meis1-primary-antibodies-wb-testing-1_1.jpgAnti-MEIS1 Monoclonal AntibodyWestern blot analysis of MEIS1 using anti-MEIS1 antibody (M01602). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human HEL whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIS1 antigen affinity purified monoclonal antibody (M01602) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEIS1 at approximately 45,53 kDa. The expected band size for MEIS1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01602-meis1-primary-antibodies-wb-testing-2_1.jpgAnti-MEIS1 Monoclonal AntibodyWestern blot analysis of MEIS1 using anti-MEIS1 antibody (M01602). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human HEL whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIS1 antigen affinity purified monoclonal antibody (M01602) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEIS1 at approximately 45,53 kDa. The expected band size for MEIS1 is at 43 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkc-epsilon-antibody-m01151-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01151-wb.jpgAnti-PKC epsilon Monoclonal AntibodyWestern blot analysis of PKC epsilon expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01151-wb7.jpgAnti-PKC epsilon Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01151-wb8.jpgAnti-PKC epsilon Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klkb1-antibody-m04995-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04995-wb.jpgAnti-KLKB1 Monoclonal AntibodyWestern blot analysis of KLKB1 expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbp4-antibody-m01089-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01089-rbp4-primary-antibodies-wb-testing-1_1.jpgAnti-RBP4 Monoclonal AntibodyWestern blot analysis of RBP4 using anti-RBP4 antibody (M01089). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br>
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP4 antigen affinity purified monoclonal antibody (M01089) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBP4 at approximately 23 kDa. The expected band size for RBP4 is at 23 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tctp-antibody-m03414-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03414-wb.jpgAnti-TCTP Rabbit Monoclonal AntibodyWestern blot analysis of TCTP Receptor expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytochrome-p450-3a4-antibody-m00339-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00339-wb.jpgAnti-Cytochrome P450 3A4 CYP3A4 Monoclonal AntibodyWestern blot analysis of Cytochrome P450 3A4 expression in Human fetal liver lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adora1-antibody-m01101-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01101-wb.jpgAnti-ADORA1 Rabbit Monoclonal AntibodyWestern blot analysis of ADORA1 expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rock2-antibody-m01023-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01023-rock2-primary-antibodies-wb-testing-1.jpgAnti-ROCK2 Monoclonal Antibody Western blot analysis of ROCK2 using anti-ROCK2 antibody (M01023). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human SH-SY5Y whole cell lysates,<br>
Lane 5: rat lung tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse lung tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified monoclonal antibody (Catalog # M01023) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161 kDa. The expected band size for ROCK2 is at 161 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cenpe-antibody-m04553-1-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04553-1-wb.jpgAnti-CENPE Monoclonal AntibodyWestern blot analysis of CENPE expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kif3a-antibody-m03439-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03439-wb.jpgAnti-KIF3A Monoclonal AntibodyWestern blot analysis of KIF3A expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddx58-antibody-m00244-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00244-ddx58-primary-antibodies-wb-testing-1.jpgAnti-DDX58 Monoclonal Antibody Western blot analysis of DDX58 using anti-DDX58 antibody (M00244). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human SIHA whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified monoclonal antibody (M00244) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 107 kDa. The expected band size for DDX58 is at 107 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddr2-antibody-m01698-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01698-ddr2-primary-antibodies-wb-testing-1.jpgAnti-DDR2 Monoclonal AntibodyWestern blot analysis of DDR2 using anti-DDR2 antibody (M01698). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human U2OS whole cell lysates,<br>
Lane 4: rat C6 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified monoclonal antibody (M01698) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 100 kDa. The expected band size for DDR2 is at 97 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-dqa1-antibody-m00232-1-boster.html2026-03-24T05:19:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00232-1-wb.jpgAnti-HLA-DQA1 Monoclonal AntibodyWestern blot analysis of HLA-DQA1 expression in human spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pf4-antibody-m00871-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-pf4-primary-antibodies-wb-testing-1.jpgAnti-PF4 Monoclonal Antibody Western blot analysis of PF4 using anti-PF4 antibody (M00871). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates,<br>
Lane 2: rat lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified monoclonal antibody (Catalog # M00871) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 15 kDa. The expected band size for PF4 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-wb.jpgAnti-PF4 Monoclonal AntibodyWestern blot analysis of PF4 expression in human spleen lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-pf4-primary-antibodies-ihc-testing-2.jpgAnti-PF4 Monoclonal Antibody IHC analysis of PF4 using anti-PF4 antibody (M00871). <br>
PF4 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PF4 Antibody (M00871) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-pf4-primary-antibodies-ihc-testing-3.jpgAnti-PF4 Monoclonal Antibody IHC analysis of PF4 using anti-PF4 antibody (M00871). <br>
PF4 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PF4 Antibody (M00871) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-pf4-primary-antibodies-ihc-testing-4.jpgAnti-PF4 Monoclonal Antibody IHC analysis of PF4 using anti-PF4 antibody (M00871). <br>
PF4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PF4 Antibody (M00871) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00871-pf4-primary-antibodies-ihc-testing-5.jpgAnti-PF4 Monoclonal Antibody IHC analysis of PF4 using anti-PF4 antibody (M00871). <br>
PF4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PF4 Antibody (M00871) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-msin3a-antibody-m01203-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01203-wb.jpgAnti-mSin3A Rabbit Monoclonal AntibodyWestern blot analysis of mSin3A expression in (1) K562 cell lysate; (2) RAW 264.7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phd2-prolyl-hydroxylase-antibody-m00415-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00415-wb7.jpgAnti-PHD2 / prolyl hydroxylase Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00415-wb.jpgAnti-PHD2 / prolyl hydroxylase Monoclonal AntibodyWestern blot analysis of PHD2 / prolyl hydroxylase expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-peroxiredoxin-3-antibody-m02680-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02680-prdx3-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 3 Rabbit Monoclonal AntibodyWestern blot analysis of PRDX3 using anti-PRDX3 antibody (M02680). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates,<br>
Lane 5: human A431 whole cell lysates,<br>
Lane 6: human U251 whole cell lysates,<br>
Lane 7: human Hela whole cell lysates,<br>
Lane 8: human PC-3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX3 antigen affinity purified monoclonal antibody (M02680) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDX3 at approximately 24 kDa. The expected band size for PRDX3 is at 28 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crmp2-antibody-m02860-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02860-wb.jpgAnti-CRMP2 Rabbit Monoclonal AntibodyWestern blot analysis of CRMP2 expression in (1) U87-MG cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspr-antibody-m08617-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08617-wb.jpgAnti-Caspr Monoclonal AntibodyWestern blot analysis of Caspr expression in U87-MG cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-idh2-antibody-m00510-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00510-idh2-primary-antibodies-wb-testing-1.jpgAnti-IDH2 Monoclonal Antibody Western blot analysis of IDH2 using anti-IDH2 antibody (M00510). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MOLT-4 whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat liver tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse liver tissue lysate.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH2 antigen affinity purified monoclonal antibody (Catalog # M00510) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00510-idh2-primary-antibodies-ihc-testing-2.jpgAnti-IDH2 Monoclonal Antibody IHC analysis of IDH2 using anti-IDH2 antibody (M00510). <br>
IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IDH2 Antibody (M00510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00510-idh2-primary-antibodies-ihc-testing-3.jpgAnti-IDH2 Monoclonal Antibody IHC analysis of IDH2 using anti-IDH2 antibody (M00510). <br>
IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IDH2 Antibody (M00510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nuclear-matrix-protein-p84-antibody-m04626-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04626-thoc1-primary-antibodies-wb-testing-1.jpgAnti-Nuclear Matrix Protein p84 Monoclonal AntibodyWestern blot analysis of THOC1 using anti-THOC1 antibody (M04626). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysates,<br>
Lane 2: human SIHA whole cell lysates,<br>
Lane 3: human U251 whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse Neuro-2a tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THOC1 antigen affinity purified monoclonal antibody (M04626) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THOC1 at approximately 76 kDa. The expected band size for THOC1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04626-thoc1-primary-antibodies-ihc-testing-1.jpgAnti-Nuclear Matrix Protein p84 Monoclonal AntibodyIHC analysis of THOC1 using anti-THOC1 antibody (M04626). <br>THOC1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-THOC1 Antibody (M04626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04626-thoc1-primary-antibodies-ihc-testing-2.jpgAnti-Nuclear Matrix Protein p84 Monoclonal AntibodyIHC analysis of THOC1 using anti-THOC1 antibody (M04626). <br>THOC1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-THOC1 Antibody (M04626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04626-thoc1-primary-antibodies-ihc-testing-3.jpgAnti-Nuclear Matrix Protein p84 Monoclonal AntibodyIHC analysis of THOC1 using anti-THOC1 antibody (M04626). <br>THOC1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-THOC1 Antibody (M04626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04626-thoc1-primary-antibodies-ihc-testing-4.jpgAnti-Nuclear Matrix Protein p84 Monoclonal AntibodyIHC analysis of THOC1 using anti-THOC1 antibody (M04626). <br>THOC1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-THOC1 Antibody (M04626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zic1-antibody-m05537-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05537-wb.jpgAnti-Zic1 Monoclonal AntibodyWestern blot analysis of Zic1 expression in SW480 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcsf-receptor-cd114-antibody-m00281-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00281-wb.jpgAnti-GCSF Receptor (CD114) Monoclonal AntibodyWestern blot analysis of GCSF Receptor (CD114) expression in Caco-2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcr3-antibody-m00295-boster.html2026-03-25T05:22:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00295-wb.jpgAnti-CXCR3 Monoclonal AntibodyWestern blot analysis of CXCR3 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-reelin-antibody-m02113-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02113-wb.jpgAnti-Reelin Rabbit Monoclonal AntibodyWestern blot analysis of Reelin expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dynamin-2-antibody-m01629-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01629-wb.jpgAnti-Dynamin 2 Monoclonal AntibodyWestern blot analysis of Dynamin 2 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chromogranin-b-antibody-m04073-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04073-chgb-primary-antibodies-wb-testing-1.jpgAnti-Chromogranin B CHGB Monoclonal AntibodyWestern blot analysis of Chromogranin B/CHGB using anti-Chromogranin B/CHGB antibody (M04073). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human U2OS whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin B/CHGB antigen affinity purified monoclonal antibody (M04073) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Chromogranin B/CHGB at approximately 125 kDa. The expected band size for Chromogranin B/CHGB is at 78 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apoer2-antibody-m03444-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03444-apoer2-primary-antibodies-wb-testing-1.jpgAnti-ApoER2 Monoclonal Antibody Western blot analysis of ApoER2 using anti-ApoER2 antibody (M03444). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates,<br>
Lane 2: human U-87MG whole cell lysates,<br>
Lane 3: human A549 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2 antigen affinity purified monoclonal antibody (Catalog # M03444) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ApoER2 at approximately 106 kDa. The expected band size for ApoER2 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03444-wb7.jpgAnti-ApoER2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03444-wb8.jpgAnti-ApoER2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03444-wb.jpgAnti-ApoER2 Monoclonal AntibodyWestern blot analysis of ApoER2 expression in C6 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ran-antibody-m00204-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-wb7.jpgAnti-Ran Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-wb.jpgAnti-Ran Monoclonal AntibodyWestern blot analysis of Ran expression in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-icc1.jpgAnti-Ran Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-icc2.jpgAnti-Ran Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclophilin-f-antibody-m02803-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02803-ppif-primary-antibodies-wb-testing-1.jpgAnti-Cyclophilin F Rabbit Monoclonal Antibody Western blot analysis of Cyclophilin F using anti-Cyclophilin F antibody (M02803). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse RAW264.7 whole cell lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin F antigen affinity purified monoclonal antibody (Catalog # M02803) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin F at approximately 18 kDa. The expected band size for Cyclophilin F is at 22 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-emerin-antibody-m00714-1-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00714-1-wb.jpgAnti-Emerin Rabbit Monoclonal AntibodyWestern blot analysis of Emerin expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hp1-gamma-antibody-m01142-1-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01142-1-cbx3-primary-antibodies-wb-testing-1.jpgAnti-HP1 gamma Rabbit Monoclonal Antibody Western blot analysis of HP1 Gamma using anti-HP1 Gamma antibody (M01142-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human THP-1 whole cell lysates,<br>
Lane 4: human U-937 whole cell lysates,<br>
Lane 5: rat lung tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse lung tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 Gamma antigen affinity purified monoclonal antibody (Catalog # M01142-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 Gamma at approximately 21 kDa. The expected band size for HP1 Gamma is at 21 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-factor-xiiia-antibody-m02047-1-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02047-1-f13a1-primary-antibodies-wb-testing-1.jpgAnti-Factor XIIIa F13A1 Monoclonal AntibodyWestern blot analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (M02047-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>
Lane 2: human THP-1 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor XIIIa/F13A1 antigen affinity purified monoclonal antibody (M02047-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Factor XIIIa/F13A1 at approximately 83 kDa. The expected band size for Factor XIIIa/F13A1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02047-1-f13a1-primary-antibodies-ihc-testing-1.jpgAnti-Factor XIIIa F13A1 Monoclonal AntibodyIHC analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (M02047-1). <br>Factor XIIIa/F13A1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Factor XIIIa/F13A1 Antibody (M02047-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02047-1-f13a1-primary-antibodies-ihc-testing-2.jpgAnti-Factor XIIIa F13A1 Monoclonal AntibodyIHC analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (M02047-1). <br>Factor XIIIa/F13A1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Factor XIIIa/F13A1 Antibody (M02047-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02047-1-f13a1-primary-antibodies-ihc-testing-3.jpgAnti-Factor XIIIa F13A1 Monoclonal AntibodyIHC analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (M02047-1). <br>Factor XIIIa/F13A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Factor XIIIa/F13A1 Antibody (M02047-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02047-1-f13a1-primary-antibodies-ihc-testing-4.jpgAnti-Factor XIIIa F13A1 Monoclonal AntibodyIHC analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (M02047-1). <br>Factor XIIIa/F13A1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Factor XIIIa/F13A1 Antibody (M02047-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-opa1-antibody-m00508-boster.html2026-03-24T05:19:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00508-wb.jpgAnti-OPA1 Monoclonal AntibodyWestern blot analysis of OPA1 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-edg2-antibody-m02782-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02782-lpar1-primary-antibodies-wb-testing-1_1.jpgAnti-EDG2 LPAR1 Rabbit Monoclonal AntibodyWestern blot analysis of LPAR1 using anti-LPAR1 antibody (M02782). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPAR1 antigen affinity purified monoclonal antibody (M02782) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LPAR1 at approximately 39 kDa. The expected band size for LPAR1 is at 39 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slit2-antibody-m01627-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01627-wb7.jpgAnti-Slit2 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01627-wb10.jpgAnti-Slit2 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01627-wb8.jpgAnti-Slit2 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01627-wb.jpgAnti-Slit2 Monoclonal AntibodyWestern blot analysis of Slit2 expression in SH-SY5Y cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01627-wb9.jpgAnti-Slit2 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gal4-antibody-m08468-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08468-wb.jpgAnti-GAL4 Monoclonal AntibodyWestern blot analysis of GAL4 expression in rat small intestine lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08468-lgals4-primary-antibodies-ihc-testing-1.jpgAnti-GAL4 Monoclonal AntibodyIHC analysis of Galectin-4/LGALS4 using anti-Galectin-4/LGALS4 antibody (M08468). <br>Galectin-4/LGALS4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Galectin-4/LGALS4 Antibody (M08468) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08468-lgals4-primary-antibodies-ihc-testing-2.jpgAnti-GAL4 Monoclonal AntibodyIHC analysis of Galectin-4/LGALS4 using anti-Galectin-4/LGALS4 antibody (M08468). <br>Galectin-4/LGALS4 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Galectin-4/LGALS4 Antibody (M08468) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dna-polymerase-beta-antibody-m01946-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01946-wb7.jpgAnti-DNA Polymerase beta Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01946-wb8.jpgAnti-DNA Polymerase beta Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01946-wb.jpgAnti-DNA Polymerase beta Monoclonal AntibodyWestern blot analysis of DNA Polymerase beta expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xct-antibody-m03036-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-wb7_1.jpgAnti-xCT Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-fonc-12-874900-g004.jpgAnti-xCT Rabbit Monoclonal AntibodySS attenuated the oxidation resistance of LUAD cells. (A, B) GSH or Cys was detected in Calu-1 or A549 cells, which were respectively treated with 10, 15, 20 μM or 20, 25, 30 μM SS for 6 h, and pretreated with or without Fer-1 (1 μM), the data statistic was shown in a histogram (*P < 0.05, **P < 0.01, ***P < 0.001). (C) Western blot analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 10, 15, 20 μM SS for 6 h. (D) Quantitative analysis of gray value of the SLC7A11 and GPX4 blots. (E) Western blotting analysis was used to detect the expressions of SLC7A11 and GPX4 in Calu-1 cells, which were treated with 20 μM SS or 4 μM erastin, with or without Fer-1 (1 μM) for 6 h. (F) Quantitative analysis of gray value of the SLC7A11 and GPX4 blots.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9158126/&orig_host=www.ncbi.nlm.nih.gov'>35664792</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-fonc-15-1620122-g006.jpgAnti-xCT Rabbit Monoclonal AntibodyAKT inhibition induced MDA-MB-231 pyroptosis and decrease SLC7A11 expression. (A) Western blot analysis of NLRP3, GSDMD, caspase-1, AKT, p-AKT, PI3K, p-PI3K, mTOR and p-mTOR expression in MDA-MB-231 cells treated with AKT inhibitor or control buffer. * p <0.05, ** p < 0.01. Statistical analysis of the two groups were performed using two-tailed Student’s t test. The concentration of IL1β (B) and IL18 (C) in cell culture supernatant were all significantly elevated in the MK-2206-treated group, compared to the control group ( p = 0.0134 and p = 0.0015, respectively, t -test). (D) Analysis of pathways and co-expression data of SLC7A11, EHPA2, pyroptosis related genes and AKT/PI3K/mTOR related genes. Relative mRNA expression levels ( p = 0.0002, t -test) (E) and protein expression levels (F) of ferroptosis-associated gene SLC7A11 were all decreased in shEPHA2–1 group. Bars represent the mean ± SD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12411449/'>40919148</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-fphar-15-1378634-g004.jpgAnti-xCT Rabbit Monoclonal AntibodyAA and PC exerted synergistic effects on regulating GSH metabolism. (A) IHC analysis of SLC7A11 and GPX4 in the femur. (B) GSH/GSSG ratio in tibias. Data are expressed as the mean ± SD (n = 5). ** p < 0.01 compared with the OVX group. ## p < 0.01 compared with the AA/PC group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1378634/full'>39512823</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-icc2.jpgAnti-xCT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-41598_2024_80160_fig2_html.pngAnti-xCT Rabbit Monoclonal AntibodyA correlation between the effect of MT1G on GC cell lines and the occurrence of ferroptosis. ( a ) MDA expression in different GC cell lines. ( b ) The expression of SOD in different GC cell line. ( c ) The expression of GSH in different GC cell lines. ( d ) The expression of ferroptosis related proteins in different GC cell lines. ( e ) The expression of GPX4 protein in different GC cell lines. ( f ) The expression of FSP1 protein in different GC cell lines. ( g ) Expression of SLC7A11 protein in different GC cell lines. ( h ) The expression of DHODH protein in different GC cell lines. ( i ) Fluorescence intensity of Fe 2+ content. Scale bar = 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-80160-4'>39558129</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-icc3.jpgAnti-xCT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-jcav16p1598g007.jpgAnti-xCT Rabbit Monoclonal AntibodyM2c macrophages increase ferroptosis resistance in gastric cancer cells. a The CCK-8 method was used to detect the survival of gastric cancer cells (Hgc27 and MKN45) intervened with RSL3 for 24 h. b The CCK-8 method was used to detect the survival of gastric cancer cells (Hgc27 and MKN45) intervened with Fer-1 for 24 h. c The expression of SOD in different intervention groups. d The expression of MDA in different intervention groups. e The expression of GSH in different intervention groups. f The expression of TGFβ1 protein WB in different cell lines. g The expression results of TGFβ1 protein. h The expression of key ferroptosis proteins WB in different cell lines. i The expression results of FSP1 protein. j Expression results of DHODH protein. k Expression results of GPX4 protein. l SLC7A11 protein expression results. m The intervention of RSL3 on the expression of key ferroptosis protein WB in different co culture groups. n The expression results of GPX4 protein. o SLC7A11 protein expression results. p The WB expression of key proteins involved in ferroptosis in different intervention groups. q The expression results of GPX4 protein. r The expression results of SLC7A11 protein. s Fluorescence results of mitochondrial membrane potential in different intervention groups. Scale bar=50 μm. *p<0.05, **p<0.01, ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11843238/'>39991579</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-icc4.jpgAnti-xCT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-wb8_1.jpgAnti-xCT Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1000 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-icc1.jpgAnti-xCT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-wb_1.jpgAnti-xCT Rabbit Monoclonal AntibodyWestern blot analysis of xCT expression in (1) HepG2 cell lysate; (2) Mouse brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-xct-primary-antibodies-wb-testing-2.jpgAnti-xCT Rabbit Monoclonal AntibodyWestern blot analysis of SLC7A11 using anti-SLC7A11 antibody (M03036).<br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br>
Lane 1: Human U-87MG whole cell lysates,<br>
Lane 2: Human LN229 whole cell lysates,<br>
Lane 3: Rat C6 whole cell lysates,<br>
Lane 4: Rat RG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A11 antigen affinity purified monoclonal antibody (M03036) at a dilution of 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A11 at approximately 60 kDa. The expected band size for SLC7A11 is at 55 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-xct-primary-antibodies-icc-testing-1.jpgAnti-xCT Rabbit Monoclonal AntibodyICC/IF analysis of SLC7A11 using anti-SLC7A11 antibody (M03036). <br>
SLC7A11 was detected in an immunocytochemical section of rat C6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-SLC7A11 Antibody (M03036) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-coup-tf1-antibody-m03833-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-wb-testing-1.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyWestern blot analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2F1 antigen affinity purified monoclonal antibody (M03833) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NR2F1 at approximately 46 kDa. The expected band size for NR2F1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-7.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-5.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-6.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-4.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-3.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03833-nr2f1-primary-antibodies-ihc-testing-2.jpgAnti-COUP TF1 NR2F1 Monoclonal AntibodyIHC analysis of NR2F1 using anti-NR2F1 antibody (M03833). <br>NR2F1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NR2F1 Antibody (M03833) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atg7-apg7-antibody-m00346-1-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-1-atg7-primary-antibodies-wb-testing-1.jpgAnti-Atg7 (Apg7) Monoclonal Antibody Western blot analysis of Atg7 using anti-Atg7 antibody (M00346-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human SIHA whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Atg7 antigen affinity purified monoclonal antibody (M00346-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Atg7 at approximately 78 kDa. The expected band size for Atg7 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-1-wb7.jpgAnti-Atg7 (Apg7) Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-1-wb.jpgAnti-Atg7 (Apg7) Monoclonal AntibodyWestern blot analysis of Atg7(Apg7) expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate; (3) Rat kidney lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-1-atg7-primary-antibodies-wb-testing-2.jpgAnti-Atg7 (Apg7) Monoclonal Antibody Western blot analysis of Atg7 using anti-Atg7 antibody (M00346-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela- WT whole cell lysates,<br>
Lane 2: human Hela-GPX4 KO whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-Atg7 antigen affinity purified monoclonal antibody (M00346-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Atg7 at approximately 78 kDa. The expected band size for Atg7 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-1-icc1.jpgAnti-Atg7 (Apg7) Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atg4a-antibody-m06539-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06539-atg4a-primary-antibodies-wb-testing-1_1.jpgAnti-Atg4A Monoclonal Antibody Western blot analysis of Atg4A using anti-Atg4A antibody (M06539). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human T-47D whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse C2C12 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Atg4A antigen affinity purified monoclonal antibody (Catalog # M06539) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Atg4A at approximately 45 kDa. The expected band size for Atg4A is at 45 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ulk3-antibody-m07706-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07706-wb.jpgAnti-ULK3 Monoclonal AntibodyWestern blot analysis of ULK3 expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pras40-antibody-m03629-1-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03629-1-wb7.jpgAnti-PRAS40 AKT1S1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03629-1-wb.jpgAnti-PRAS40 AKT1S1 Monoclonal AntibodyWestern blot analysis of PRAS40 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03629-1-wb8.jpgAnti-PRAS40 AKT1S1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03629-1-wb9.jpgAnti-PRAS40 AKT1S1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gabarap-antibody-m01907-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01907-gabarap-primary-antibodies-wb-testing-1.jpgAnti-GABARAP Monoclonal Antibody Western blot analysis of GABARAP using anti-GABARAP antibody (M01907). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human HUH7 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat liver tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified monoclonal antibody (Catalog # M01907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18 kDa. The expected band size for GABARAP is at 14 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-map3k7-antibody-m01458-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01458-map3k7-primary-antibodies-wb-testing-1.jpgAnti-MAP3K7 Monoclonal Antibody Western blot analysis of MAP3K7 using anti-MAP3K7 antibody (M01458). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: rat lung tissue lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse lung tissue lysates,<br>
Lane 7: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K7 antigen affinity purified monoclonal antibody (Catalog # M01458) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K7 at approximately 75 kDa. The expected band size for MAP3K7 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01458-42003_2023_4711_fig5_html.pngAnti-MAP3K7 Monoclonal Antibodycalpain2a inhibits TRAF6 ubiquitin-ligase activity. a Ubiquitination of endogenous TRAF6 in MKC cells transduced with calpain2a-Flag and calpain2a-ΔCysPc-Flag and unchallenged (−) or challenged with LPS (+), assessed by immunoblot analysis with anti-ubiquitin after immunoprecipitation with anti-TRAF6 and input immunoblot analysis with indicated Abs. b , c HEK293 cells were cotransfected with TRAF6-HA and WT-ubiquitin-His or K63O-ubiquitin-His (in which only lysine 63 is kept) together with calpain2a-Flag, calpain2a-ΔCysPc-Flag or empty vector. After 24 h post-transfection, the cells were lysed and purified with Ni-NTA agarose. d , e HEK293 cells were cotransfected with TRAF6-HA and WT-ubiquitin-His or K63O-ubiquitin-His (in which only lysine 63 is kept) together with calpain2a-Flag (0.5 μg, 1 μg), calpain2a-ΔCysPc-Flag (0.5 μg, 1 μg) or empty vector. After 24 h post-transfection, the cells were lysed and purified with Ni-NTA agarose. f Ubiquitination of overexpressed TRAF6 in MKC cells transduced with calpain2a-ΔCysPc-Flag, assessed by immunoblot analysis with anti-ubiquitin after immunoprecipitation with anti-Myc and input immunoblot analysis with indicated Abs. g , h HEK293 cells were cotransfected with TAK1-Myc, TRAF6-HA and WT-ubiquitin-His or K63O-ubiquitin-His (in which only lysine 63 is kept) together with calpain2a-Flag, calpain2a-ΔCysPc-Flag or empty vector. After 24 h post-transfection, the cells were lysed and purified with Ni-NTA agarose. All the immunoprecipitates and input immunoblot analysis with anti-Myc, anti-Flag, anti-HA, and anti-Tubulin Abs. All experiments were performed in at least three independent experiments. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs42003-023-04711-7'>37002312</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01458-42003_2023_4711_fig7_html.pngAnti-MAP3K7 Monoclonal Antibodycalpain2a inhibits TRAF6-mediated ubiquitination of ECSIT and BECN1. a HEK293 cells were transfected with ECSIT-Myc, TRAF6-Flag, or empty vector. After 24 h post-transfection, the cells were lysed and IP analyses with Flag antibody. b HEK293 cells were transfected with TAK1-Flag, ECSIT-Myc, or empty vector. After 24 h post-transfection, the cells were lysed and IP analyses with Myc antibody. c HEK293 cells were transfected with TRAF6-Myc, ECSIT-HA, empty vector, or different concentrations of calpain2a-ΔCysPc-Flag. After 24 h post-transfection, the cells were lysed and IP analyses with Myc antibody. d HEK293 cells were cotransfected with ECSIT-Myc, TRAF6-HA, WT-ubiquitin-His together with calpain2a-Flag, calpain2a-ΔCysPc-Flag or empty vector. After 24 h post-transfection, the cells were lysed and purified with Ni-NTA agarose. e HEK293 cells were transfected with BECN1-Flag, TRAF6-Myc, or empty vector. After 24 h post-transfection, the cells were lysed and IP analyses with Myc antibody. f HEK293 cells were cotransfected with BECN1-HA, TRAF6-Myc, WT-ubiquitin-His together with calpain2a-Flag, calpain2a-ΔCysPc-Flag or empty vector. After 24 h post-transfection, the cells were lysed and purified with Ni-NTA agarose. The immunoprecipitates and input with anti-Myc, anti-Flag, anti-HA, and anti-Tubulin Abs. All experiments were performed in at least three independent experiments. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs42003-023-04711-7'>37002312</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01458-if.jpgAnti-MAP3K7 Monoclonal AntibodyImmunofluorescent analysis of A431 cells, using TAK1 Antibody .
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dynamin-1-antibody-m02536-1-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02536-1-wb.jpgAnti-Dynamin 1 DNM1 Rabbit Monoclonal AntibodyWestern blot analysis of Dynamin 1 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trefoil-factor-3-antibody-m01738-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01738-tff3-primary-antibodies-wb-testing-1.jpgAnti-Trefoil Factor 3 Monoclonal AntibodyWestern blot analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (M01738). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trefoil factor 3/TFF3 antigen affinity purified monoclonal antibody (M01738) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Trefoil factor 3/TFF3 at approximately 13 kDa. The expected band size for Trefoil factor 3/TFF3 is at 10 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-actinin-4-antibody-m01975-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01975-wb.jpgAnti-alpha Actinin 4 ACTN4 Monoclonal AntibodyWestern blot analysis of alpha Actinin 4 expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-human-igg3-antibody-m08374-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08374-wb.jpgAnti-Human IgG3 IGHG3 Monoclonal AntibodyWestern blot analysis of Human IgG3 expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glur2-antibody-m02905-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-wb-testing-1.jpgAnti-GluR2 Monoclonal AntibodyWestern blot analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUR2 antigen affinity purified monoclonal antibody (M02905) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLUR2 at approximately 99 kDa. The expected band size for GLUR2 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-ihc-testing-1.jpgAnti-GluR2 Monoclonal AntibodyIHC analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>GLUR2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUR2 Antibody (M02905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-ihc-testing-2.jpgAnti-GluR2 Monoclonal AntibodyIHC analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>GLUR2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUR2 Antibody (M02905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-ihc-testing-3.jpgAnti-GluR2 Monoclonal AntibodyIHC analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>GLUR2 was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUR2 Antibody (M02905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-ihc-testing-4.jpgAnti-GluR2 Monoclonal AntibodyIHC analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>GLUR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUR2 Antibody (M02905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02905-glur2-primary-antibodies-ihc-testing-5.jpgAnti-GluR2 Monoclonal AntibodyIHC analysis of GLUR2 using anti-GLUR2 antibody (M02905). <br>GLUR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUR2 Antibody (M02905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pmp22-antibody-m00890-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00890-wb7.jpgAnti-PMP22 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00890-wb.jpgAnti-PMP22 Monoclonal AntibodyWestern blot analysis of PMP22 expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myelin-oligodendrocyte-glycoprotein-antibody-m03294-boster.html2026-03-24T05:19:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03294-mog-primary-antibodies-wb-testing-1.jpgAnti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody Western blot analysis of MOG using anti-MOG antibody (M03294). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOG antigen affinity purified monoclonal antibody (M03294) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MOG at approximately 25 kDa. The expected band size for MOG is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03294-mog-primary-antibodies-ihc-testing-2.jpgAnti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody IHC analysis of MOG using anti-MOG antibody (M03294). <br>MOG was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-MOG Antibody (M03294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03294-mog-primary-antibodies-ihc-testing-3.jpgAnti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody IHC analysis of MOG using anti-MOG antibody (M03294). <br>MOG was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-MOG Antibody (M03294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03294-mog-primary-antibodies-ihc-testing-4.jpgAnti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody IHC analysis of MOG using anti-MOG antibody (M03294). <br>MOG was detected in a paraffin-embedded section of rat hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-MOG Antibody (M03294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm7-antibody-m01649-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-1-mcm7-primary-antibodies-wb-testing-1.jpgAnti-MCM7 Monoclonal AntibodyWestern blot analysis of MCM7 using anti-MCM7 antibody (M01649-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified monoclonal antibody (M01649-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-1-mcm7-primary-antibodies-ihc-testing-1.jpgAnti-MCM7 Monoclonal AntibodyIHC analysis of MCM7 using anti-MCM7 antibody (M01649-1). <br>MCM7 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MCM7 Antibody (M01649-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hb9-hlxb9-antibody-m04520-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04520-wb.jpgAnti-HB9/HLXB9 Monoclonal AntibodyWestern blot analysis of HB9/HLXB9 expression in Molt-4 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prolactin-receptor-antibody-m01196-2-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01196-2-wb7.jpgAnti-Prolactin Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01196-2-wb10.jpgAnti-Prolactin Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01196-2-wb8.jpgAnti-Prolactin Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01196-2-wb.jpgAnti-Prolactin Receptor Rabbit Monoclonal AntibodyWestern blot analysis of Prolactin Receptor expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01196-2-wb9.jpgAnti-Prolactin Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufs3-antibody-m05867-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05867-ndufs3-primary-antibodies-wb-testing-1_1.jpgAnti-NDUFS3 Monoclonal AntibodyWestern blot analysis of NDUFS3 using anti-NDUFS3 antibody (M05867). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human Caco-2 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS3 antigen affinity purified monoclonal antibody (M05867) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDUFS3 at approximately 27 kDa. The expected band size for NDUFS3 is at 30 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-msh2-antibody-m00140-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-wb.jpgAnti-MSH2 Monoclonal AntibodyWestern blot analysis of MSH2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd5-antibody-m00480-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00480-1-wb.jpgAnti-CD5 Monoclonal AntibodyWestern blot analysis of CD5 expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd79a-antibody-m01047-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01047-1-wb7.jpgAnti-CD79a Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01047-1-wb8.jpgAnti-CD79a Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01047-1-wb.jpgAnti-CD79a Monoclonal AntibodyWestern blot analysis of CD79a expression in human spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fascin-antibody-m02147-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02147-1-fascin-primary-antibodies-wb-testing-1.jpgAnti-Fascin Monoclonal Antibody Western blot analysis of Fascin using anti-Fascin antibody (M02147-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fascin antigen affinity purified monoclonal antibody (Catalog # M02147-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fascin at approximately 55 kDa. The expected band size for Fascin is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02147-1-fascin-primary-antibodies-ihc-testing-2.jpgAnti-Fascin Monoclonal Antibody IHC analysis of Fascin using anti-Fascin antibody (M02147-1). <br>
Fascin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fascin Antibody (M02147-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02147-1-fascin-primary-antibodies-ihc-testing-3.jpgAnti-Fascin Monoclonal Antibody IHC analysis of Fascin using anti-Fascin antibody (M02147-1). <br>
Fascin was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fascin Antibody (M02147-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02147-1-fascin-primary-antibodies-ihc-testing-4.jpgAnti-Fascin Monoclonal Antibody IHC analysis of Fascin using anti-Fascin antibody (M02147-1). <br>
Fascin was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fascin Antibody (M02147-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd82-antibody-m02300-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02300-wb.jpgAnti-CD82 Monoclonal AntibodyWestern blot analysis of CD82 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lambda-light-chain-antibody-m13347-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13347-wb.jpgAnti-Lambda Light chain IGLV1-51 Monoclonal AntibodyWestern blot analysis of Lambda Light chain expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calretinin-antibody-m04255-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04255-wb.jpgAnti-Calretinin Monoclonal AntibodyWestern blot analysis of Calretinin expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndrg1-antibody-m01327-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01327-1-ndrg1-primary-antibodies-wb-testing-1.jpgAnti-NDRG1 Monoclonal Antibody Western blot analysis of NDRG1 using anti-NDRG1 antibody (M01327-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified monoclonal antibody (M01327-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 43 kDa. The expected band size for NDRG1 is at 43 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mum1-antibody-m00401-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00401-wb.jpgAnti-MUM1 Monoclonal AntibodyWestern blot analysis of MUM1 expression in Daudi cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glycophorin-a-cd235a-antibody-m02184-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-wb.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyWestern blot analysis of Glycophorin A (CD235a) expression in human fetal liver lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-wb7.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-wb8.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-gypa-primary-antibodies-ihc-testing-1.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal Antibody analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1). <br>Glycophorin A/GYPA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-gypa-primary-antibodies-ihc-testing-2.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyIHC analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1). <br>Glycophorin A/GYPA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02184-1-gypa-primary-antibodies-ihc-testing-3.jpgAnti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyIHC analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1). <br>
Glycophorin A/GYPA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-isg15-antibody-m00554-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-wb.jpgAnti-ISG15 Monoclonal AntibodyWestern blot analysis of ISG15 expression in Hela cell lysate treated with IFN α.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f1.jpgAnti-ISG15 Monoclonal AntibodyThe expression of ISG15 across cancers at a mRNA level in the TCGA and GTEx databases. A. ISG15 expression in normal and tumor tissues in paired samples. B. ISG15 expression in normal and tumor tissues in unpaired samples.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f2.jpgAnti-ISG15 Monoclonal AntibodyISG15 expressions across cancers at a protein level in the HPA database. (A) Lung, (B) Live, (C) Colon, (D) Pancreas, (E) Brain, (F) Stomach, (G) Cervix, (H) Breast, (I) Lymph, (J) Prostate.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f3.jpgAnti-ISG15 Monoclonal AntibodyCorrelation of ISG15 expression with prognosis of cancer patients. Correlations between ISG15 expression and the OS of (A) LUAD, (B) LIHC, (C) COADREAD, (D) PAAD, (E) GBMLGG, (F) UVM, (G) LAML, (H) ACC, and (I) KIRC were analyzed using the TCGA database.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f6.jpgAnti-ISG15 Monoclonal AntibodyScatter plot showing the correlation between the expression of ISG15 and immune cell. (A) aDC, (B) B cells, (C) CD8 T cells, (D) Cytotoxic cells, (E) DC, (F) iDC, (G) Macrophages, (H) Neutrophils, (I) NK CD56bright cells, (J) NK CD56dim cells, (K) NK cells, (L) pDC, (M) T cells, (N) Tem, (O) TFH, (P) Th1 cells, (Q) Th2 cells, (R) Th17 cells and (S) TReg.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f7.jpgAnti-ISG15 Monoclonal AntibodyExpression of ISG15 in clinical samples of osteosarcoma. A. The mRNA levels of ISG15 in 19 paired samples of adjacent/normal tissues and osteosarcoma tissues were determined by qRT-PCR. B. The mRNA levels of ISG15 in unpaired samples (28 tumor tissues and 19 adjacent/normal tissues) were determined by qRT-PCR. C. ISG15 expression in adjacent/normal tissue, osteosarcoma tissue with or without lung metastasis and in pulmonary metastatic lesions were determined by immunohistochemistry (200×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f8.jpgAnti-ISG15 Monoclonal AntibodyEffects of ISG15 expression on proliferation, migration and invasion of osteosarcoma cells. A. 143B and U2OS cells were transfected with lentivirus of OE-ISG15 or sh-ISG15, and the level of ISG15 was assessed by qRT-PCR. B. 143B and U2OS cells were transfected with lentivirus of OE-ISG15 or sh-ISG15, and the level of ISG15 was assessed by Western blot. C. The proliferation ability of osteosarcoma cells was examined by colony formation assay. D. The migration abilities of osteosarcoma cells were detected by wound healing assay. E. The invasion abilities of osteosarcoma cells were detected by transwell assay.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-ajtr0015-0817-f9.jpgAnti-ISG15 Monoclonal AntibodyThe correlation between the ISG15 level and the infiltration of CD8+ T cells/CD68+ cells in osteosarcoma. A. The expression of ISG15 were negatively associated with the number of infiltrating CD8+ T cells and positively associated with CD68+ cells, as determined by double-labeled immunofluorescence (200×). B. Chemotaxis assays and flow cytometry showed that the overexpression of ISG15 induced chemotaxis of CD68+ cells (P<0.0001) but reduced the chemotaxis of CD8+ T cells (P<0.0001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006750/'>36915723</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-insulin-receptor-r-antibody-m08212-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08212-wb.jpgAnti-Insulin Receptor R INSRR Monoclonal AntibodyWestern blot analysis of Insulin Receptor R expression in Hela cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ets1-antibody-m00931-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00931-1-wb.jpgAnti-ETS1 Monoclonal AntibodyWestern blot analysis of Insulin Receptor R expression in Daudi cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thymidylate-synthase-antibody-m04320-1-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04320-1-tyms-primary-antibodies-wb-testing-1_1.jpgAnti-Thymidylate Synthase TYMS Monoclonal Antibody Western blot analysis of TYMS using anti-TYMS antibody (M04320-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: monkey COS-7 whole cell lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse thymus tissue lysates,<br>
Lane 8: mouse SP2/0 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified monoclonal antibody (Catalog # M04320-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYMS at approximately 36 kDa. The expected band size for TYMS is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04320-1-tyms-primary-antibodies-ihc-testing-2.jpgAnti-Thymidylate Synthase TYMS Monoclonal Antibody IHC analysis of TYMS using anti-TYMS antibody (M04320-1). <br>
TYMS was detected in a paraffin-embedded section of human B lymphoblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TYMS Antibody (M04320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04320-1-tyms-primary-antibodies-ihc-testing-3.jpgAnti-Thymidylate Synthase TYMS Monoclonal Antibody IHC analysis of TYMS using anti-TYMS antibody (M04320-1). <br>
TYMS was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TYMS Antibody (M04320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04320-1-tyms-primary-antibodies-ihc-testing-4.jpgAnti-Thymidylate Synthase TYMS Monoclonal Antibody IHC analysis of TYMS using anti-TYMS antibody (M04320-1). <br>
TYMS was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TYMS Antibody (M04320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hprt-antibody-m00668-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00668-wb7.jpgAnti-HPRT HPRT1 Monoclonal AntibodyAll lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00668-wb8.jpgAnti-HPRT HPRT1 Monoclonal AntibodyAll lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00668-wb.jpgAnti-HPRT HPRT1 Monoclonal AntibodyWestern blot analysis of HPRT expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fragilis-antibody-m02265-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02265-wb.jpgAnti-Fragilis IFITM3 Monoclonal AntibodyWestern blot analysis of Fragilis expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hemopexin-antibody-m02237-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02237-fonc-11-621806-g007.jpgAnti-Hemopexin HPX Monoclonal AntibodyImmunohistochemistry of FGA, F2, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CTH, CFHR3, ENNP1, and NAT2 in clinical GBC and control specimens.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.621806/full'>33718182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02237-hpx-primary-antibodies-wb-testing-1.jpgAnti-Hemopexin HPX Monoclonal AntibodyWestern blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (M02237). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Caco-2 whole cell lysates,<br>
Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified monoclonal antibody (M02237) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 70 kDa. The expected band size for Hemopexin/HPX is at 52 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kat7-hbo1-myst2-antibody-m03896-boster.html2026-03-24T05:19:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03896-wb.jpgAnti-KAT7 / HBO1 / MYST2 Monoclonal AntibodyWestern blot analysis of KAT7 / HBO1 / MYST2 expression in (1)MCF7 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kat9-elp3-antibody-m02833-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02833-wb7.jpgAnti-KAT9 / Elp3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02833-wb8.jpgAnti-KAT9 / Elp3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02833-wb.jpgAnti-KAT9 / Elp3 Rabbit Monoclonal AntibodyWestern blot analysis of KAT9 / Elp3 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) Rat kidney lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lsd2-aof1-antibody-m08071-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08071-kdm1b-primary-antibodies-wb-testing-1.jpgAnti-LSD2 / AOF1 Monoclonal Antibody Western blot analysis of KDM1B using anti-KDM1B antibody (M08071). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM1B antigen affinity purified monoclonal antibody (Catalog # M08071) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM1B at approximately 93 kDa. The expected band size for KDM1B is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08071-wb.jpgAnti-LSD2 / AOF1 Monoclonal AntibodyWestern blot analysis of LSD2 / AOF1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkc-eta-antibody-m03292-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03292-prkch-primary-antibodies-wb-testing-1.jpgAnti-PKC eta Rabbit Monoclonal Antibody Western blot analysis of PKC Eta/PRKCH using anti-PKC Eta/PRKCH antibody (M03292). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human RT4 whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: rat lung tissue lysates,<br>
Lane 4: mouse lung tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Eta/PRKCH antigen affinity purified monoclonal antibody (M03292) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKC Eta/PRKCH at approximately 78 kDa. The expected band size for PKC Eta/PRKCH is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03292-prkch-primary-antibodies-ihc-testing-2.jpgAnti-PKC eta Rabbit Monoclonal Antibody IHC analysis of PRKCH using anti-PRKCH antibody (M03292). <br>PRKCH was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKCH Antibody (M03292) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03292-prkch-primary-antibodies-ihc-testing-3.jpgAnti-PKC eta Rabbit Monoclonal Antibody IHC analysis of PRKCH using anti-PRKCH antibody (M03292). <br>PRKCH was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKCH Antibody (M03292) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phf8-antibody-m03288-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03288-phf8-primary-antibodies-wb-testing-1.jpgAnti-PHF8 Monoclonal AntibodyWestern blot analysis of PHF8 using anti-PHF8 antibody (M03288). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SIHA whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: rat testis tissue lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse testis tissue lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF8 antigen affinity purified monoclonal antibody (M03288) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHF8 at approximately 150 kDa. The expected band size for PHF8 is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03288-phf8-primary-antibodies-ihc-testing-1.jpgAnti-PHF8 Monoclonal AntibodyIHC analysis of PHF8 using anti-PHF8 antibody (M03288). <br>PHF8 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PHF8 Antibody (M03288) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03288-phf8-primary-antibodies-ihc-testing-2.jpgAnti-PHF8 Monoclonal AntibodyIHC analysis of PHF8 using anti-PHF8 antibody (M03288). <br>PHF8 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PHF8 Antibody (M03288) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03288-phf8-primary-antibodies-ihc-testing-3.jpgAnti-PHF8 Monoclonal AntibodyIHC analysis of PHF8 using anti-PHF8 antibody (M03288). <br>PHF8 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PHF8 Antibody (M03288) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytochrome-p450-2d6-antibody-m00498-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00498-wb.jpgAnti-Cytochrome P450 2D6 CYP2D6 Monoclonal AntibodyWestern blot analysis of Cytochrome P450 2D6 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube3a-antibody-m00582-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00582-wb.jpgAnti-UBE3A Monoclonal AntibodyWestern blot analysis of UBE3A expression in K562 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00582-wb9.jpgAnti-UBE3A Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00582-wb7.jpgAnti-UBE3A Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00582-wb8.jpgAnti-UBE3A Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-otx1-2-antibody-m05601-1-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05601-1-wb.jpgAnti-Otx1/2 Monoclonal AntibodyWestern blot analysis of Otx1/2 expression in Y79 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddx5-antibody-m00670-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-ddx5-primary-antibodies-wb-testing-1.jpgAnti-DDX5 Monoclonal Antibody Western blot analysis of DDX5 using anti-DDX5 antibody (M00670). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: mouse heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX5 antigen affinity purified monoclonal antibody (Catalog # M00670) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX5 at approximately 69 kDa. The expected band size for DDX5 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-ddx5-primary-antibodies-ihc-testing-2.jpgAnti-DDX5 Monoclonal Antibody IHC analysis of DDX5 using anti-DDX5 antibody (M00670). <br>
DDX5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX5 Antibody (M00670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-ddx5-primary-antibodies-ihc-testing-3.jpgAnti-DDX5 Monoclonal Antibody IHC analysis of DDX5 using anti-DDX5 antibody (M00670). <br>
DDX5 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX5 Antibody (M00670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-ddx5-primary-antibodies-ihc-testing-4.jpgAnti-DDX5 Monoclonal Antibody IHC analysis of DDX5 using anti-DDX5 antibody (M00670). <br>
DDX5 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX5 Antibody (M00670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-loxl2-antibody-m02539-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02539-wb7.jpgAnti-LOXL2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02539-wb.jpgAnti-LOXL2 Rabbit Monoclonal AntibodyWestern blot analysis of LOXL2 expression in MCF7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02539-wb8.jpgAnti-LOXL2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02539-wb9.jpgAnti-LOXL2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lck-antibody-m00425-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-wb7.jpgAnti-Lck Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-wb10.jpgAnti-Lck Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-wb8.jpgAnti-Lck Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-wb.jpgAnti-Lck Monoclonal AntibodyWestern blot analysis of Lck expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-wb9.jpgAnti-Lck Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamin-b2-antibody-m05348-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-wb-testing-1.jpgAnti-Lamin B2 Monoclonal Antibody Western blot analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hacat whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: human U251 whole cell lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse Neuro-2a whole cell lysates,<br>
Lane 8: mouse SP2/0 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2 antigen affinity purified monoclonal antibody (Catalog # M05348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2 at approximately 72 kDa. The expected band size for Lamin B2 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-2.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-3.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-4.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-5.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-6.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-7.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05348-lmnb2-primary-antibodies-ihc-testing-8.jpgAnti-Lamin B2 Monoclonal Antibody IHC analysis of Lamin B2 using anti-Lamin B2 antibody (M05348). <br>
Lamin B2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Lamin B2 Antibody (M05348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05348-LMNB2-IF-test-3.jpgAnti-Lamin B2 Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using Lamin B2 Antibody .
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uqcrc2-antibody-m07937-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-uqcrc2-primary-antibodies-wb-testing-1.jpgAnti-UQCRC2 Monoclonal AntibodyWestern blot analysis of UQCRC2 using anti-UQCRC2 antibody (M07937). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human Hacat whole cell lysates,<br>
Lane 5: rat Kidney tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRC2 antigen affinity purified monoclonal antibody (M07937) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UQCRC2 at approximately 48 kDa. The expected band size for UQCRC2 is at 48 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eef2-antibody-m00830-1-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00830-1-eef2-primary-antibodies-wb-testing-1.jpgAnti-EEF2 Monoclonal Antibody Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human SiHa whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: human K562 whole cell lysates,<br>
Lane 6: human MCF-7 whole cell lysates,<br>
Lane 7: human HEL whole cell lysates,<br>
Lane 8: human Caco-2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified monoclonal antibody (Catalog # M00830-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEF2 at approximately 100 kDa. The expected band size for EEF2 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00830-1-wb.jpgAnti-EEF2 Monoclonal AntibodyWestern blot analysis of EEF2 expression in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00830-1-eef2-primary-antibodies-ihc-testing-3.jpgAnti-EEF2 Monoclonal Antibody IHC analysis of EEF2 using anti-EEF2 antibody (M00830-1). <br>
EEF2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF2 Antibody (M00830-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00830-1-eef2-primary-antibodies-ihc-testing-4.jpgAnti-EEF2 Monoclonal Antibody IHC analysis of EEF2 using anti-EEF2 antibody (M00830-1). <br>
EEF2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF2 Antibody (M00830-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trpm8-antibody-m01002-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01002-wb7.jpgAnti-TRPM8 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01002-wb.jpgAnti-TRPM8 Monoclonal AntibodyWestern blot analysis of TRPM8 expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ace1-antibody-m00251-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00251-ace-primary-antibodies-wb-testing-1.jpgAnti-ACE1 Monoclonal Antibody Western blot analysis of ACE1 using anti-ACE1 antibody (M00251). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates,<br>
Lane 2: mouse lung tissue lysates,<br>
Lane 3: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE1 antigen affinity purified monoclonal antibody (Catalog # M00251) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE1 at approximately 180 kDa. The expected band size for ACE1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00251-wb.jpgAnti-ACE1 Monoclonal AntibodyWestern blot analysis of ACE1 expression in human fetal kidney lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tradd-antibody-m02785-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02785-wb.jpgAnti-TRADD Monoclonal AntibodyWestern blot analysis of TRADD expression in Hela cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp10-antibody-m03786-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03786-wb.jpgAnti-USP10 Monoclonal AntibodyWestern blot analysis of USP10 expression in A375 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp11-antibody-m05225-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05225-icc1.jpgAnti-USP11 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05225-usp11-primary-antibodies-wb-testing-1.jpgAnti-USP11 Monoclonal Antibody Western blot analysis of USP11 using anti-USP11 antibody (M05225). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human U20S whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse testis tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP11 antigen affinity purified monoclonal antibody (Catalog # M05225) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP11 at approximately 110 kDa. The expected band size for USP11 is at 110 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctla4-cd152-antibody-m00020-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-wb7.jpgAnti-CTLA4 (CD152) Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig1_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyHPM treatment restored body weight and increased spleen index in immunosuppressed rats. A Schematic diagram of the acupoints used in this experiment. B Dimensional specifications of the HPM in the experiment. C Schematic showing timeline for CTX induction, HPM treatment and behaviour experiments. D , E Mean weight of each group (n = 8 per each group). Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.001, *** p < 0.001 vs NG; # p < 0.05, ### p < 0.001 vs CTX. SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig2_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyImmunosuppressed rats exhibit depressive/anxiety-like behavior, which is attenuated after HPM binding inhibitor treatment. A , B Schematic representation of OFT (n = 8 per each group). C , D Schematic representation of EPM (n = 5 per each group). Data were analyzed using Kruskal–Wallis H- statistic test. * p < 0.05, ** p < 0.01vs NG. NS: no significance; OFT: open-field test; EPM: elevated plus-maze; NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig3_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyThe number of WBCs was significantly reduced in rats after cyclophosphamide injection and increased after HPM treatment. A WBCs and their subtype percentage results. (n = 3–8 per each group). B – D RBC, hemoglobin and platelet counts (n = 8 per each group). Data were analyzed using One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.01, *** p < 0.001vs NG; # p < 0.05 vs CTX; % p < 0.05 vs HPM. ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NS: no significance; NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig4_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyHistological analysis of spleen in immunosuppressed rats (H&E staining, bar = 100 μm). CV, central vein; LN, lymph nodule; MZ, marginal zone; RP, red pulp, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig5_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyLevels of CD28 ( A ), CTLA-4 ( B ), B7-1 ( C ), B7-2 ( D ), α-MSH ( E ), TrkB ( F ), 5-HT ( G ), BDNF ( H ) in serum of immunosuppressed rats (n = 8 per each group). Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CTX; %% p < 0.01, %%% p < 0.001 vs HPM. α-MSH: α-Melanocyte-stimulating hormone; TrkB: Tropomyosin receptor kinase B; 5-HT: 5-hydroxytryptamine; BDNF: Brain-derived neurotrophic factor; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig6_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyEffect of HPM treatment on T lymphocytes in the spleens of immunosuppressed rats. A CD4+, CD8+ cells were gated by the protocol. B Flow cytometric identification of CD4 + , CD8 + cells in the spleen. C , D Absolute count statistics of CD4+, CD8+ cells between different groups in spleen tissue (n = 5 per each group). Data were analyzed using Kruskal–Wallis H-statistic test. ** p < 0.01, *** p < 0.001vs NG; # p < 0.05 vs CTX. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig7_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyResults of the CD28 mRNA level and correlation between CD28 and B7-1 in spleen of immunosuppressed rats (n = 3–5 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CD28 IOD results; C CD28 mRNA level; D CD28 and B7-1 colocalization results. Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed and Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; ## p < 0.01, ### p < 0.001 vs CTX; % p < 0.05, %%% p < 0.001 vs HPM. IOD: integrated optical density; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig8_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyResults of the correlation between CD28 and B7-2 in spleen of immunosuppressed rats (n = 3 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CD28 and B7-2 colocalization results. Data are expressed as Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. ** p < 0.01 vs NG; ## p < 0.01 vs CTX. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig9_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyResults of CTLA-4 mRNA level and the correlation between CTLA-4 and B7-1 in spleen of immunosuppressed rats (n = 3–5 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CTLA-4 IOD results; C CTLA-4 mRNA level; D B7-1 mRNA level; E CTLA- and B7-1 colocalization results. Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed and Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; ## p < 0.01, ### p < 0.001 vs CTX; %%% p < 0.001 vs HPM. IOD: integrated optical density; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herbpartitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-13020_2024_898_fig10_html.pngAnti-CTLA4 (CD152) Monoclonal AntibodyResults of the correlation between CTLA-4 and B7-2 in spleen of immunosuppressed rats (n = 3 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CTLA-4 and B7-2 colocalization results. Data are expressed as Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. ## p < 0.01 vs CTX; %% p < 0.01 vs HPM. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00898-x'>38369521</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00020-wb.jpgAnti-CTLA4 (CD152) Monoclonal AntibodyWestern blot analysis of CTLA4 (CD152) expression in NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-skp2-antibody-m00544-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00544-wb.jpgAnti-SKP2 Monoclonal AntibodyWestern blot analysis of SKP2 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikb-epsilon-antibody-m07073-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07073-wb.jpgAnti-IKB epsilon NFKBIE Monoclonal AntibodyWestern blot analysis of IKB epsilon expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07073-nfkbie-primary-antibodies-ihc-testing-1_1.jpgAnti-IKB epsilon NFKBIE Monoclonal AntibodyIHC analysis of NFKBIE using anti-NFKBIE antibody (M07073). <br>NFKBIE was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NFKBIE Antibody (M07073) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp22-antibody-m03887-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03887-wb.jpgAnti-USP22 Monoclonal AntibodyWestern blot analysis of USP22 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rap80-antibody-m06262-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06262-wb.jpgAnti-RAP80 Monoclonal AntibodyWestern blot analysis of RAP80 expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-roc1-antibody-m00524-boster.html2026-03-24T05:19:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00524-wb.jpgAnti-ROC1 Monoclonal AntibodyWestern blot analysis of ROC1 expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp28-antibody-m05040-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05040-wb.jpgAnti-USP28 Monoclonal AntibodyWestern blot analysis of USP28 expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-malt1-antibody-m01599-1-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01599-1-malt1-primary-antibodies-wb-testing-1.jpgAnti-MALT1 Monoclonal AntibodyWestern blot analysis of MALT1 using anti-MALT1 antibody (M01599-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human PC-3 whole cell lysates,<br>
Lane 5: human SIHA whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MALT1 antigen affinity purified monoclonal antibody (M01599-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MALT1 at approximately 92 kDa. The expected band size for MALT1 is at 92 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fadd-antibody-m00237-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00237-wb.jpgAnti-FADD Monoclonal AntibodyWestern blot analysis of FADD expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tax1bp3-antibody-m05588-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05588-tax1bp3-primary-antibodies-wb-testing-1.jpgAnti-TAX1BP3 Monoclonal AntibodyWestern blot analysis of TAX1BP3 using anti-TAX1BP3 antibody (M05588). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HT1080 whole cell lysates,<br>
Lane 2: human SIHA whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human Hacat whole cell lysates,<br>
Lane 5: rat small intestine tissue lysates,<br>
Lane 6: rat lung tissue lysates,<br>
Lane 7: mouse lung tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAX1BP3 antigen affinity purified monoclonal antibody (M05588) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAX1BP3 at approximately 14 kDa. The expected band size for TAX1BP3 is at 14 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mda5-antibody-m00263-1-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00263-1-wb.jpgAnti-MDA5 Monoclonal AntibodyWestern blot analysis of MDA5 expression in THP1 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fadd-antibody-m00237-1-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00237-1-wb.jpgAnti-FADD Monoclonal AntibodyWestern blot analysis of FADD expression in Raw264.7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-skp1-antibody-m00476-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00476-wb.jpgAnti-Skp1 Monoclonal AntibodyWestern blot analysis of Skp1 expression in (1) 293T cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp13-antibody-m07816-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07816-wb.jpgAnti-USP13 Monoclonal AntibodyWestern blot analysis of USP13 expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-rel-antibody-m01880-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01880-rel-primary-antibodies-wb-testing-1.jpgAnti-c-Rel Monoclonal Antibody Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (M01880). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Daudi whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human RT4 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates, <br>
Lane 6: human Jurkat whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified monoclonal antibody (M01880) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-macroh2a-1-antibody-m04635-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04635-wb.jpgAnti-macroH2A.1 H2AFY Monoclonal AntibodyWestern blot analysis of macroH2A.1 expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi-3-kinase-p110-delta-antibody-m02269-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-wb7.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-wb8.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-wb9.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-wb.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyWestern blot analysis of PI 3 Kinase p110 delta expression in (1) K562 cell lysate; (2) RAW 264. cell lysate; (3) Rat kidney lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-icc1.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-icc2.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02269-icc3.jpgAnti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aspartate-aminotransferase-antibody-m04085-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-got1-primary-antibodies-wb-testing-1.jpgAnti-Aspartate Aminotransferase Monoclonal AntibodyWestern blot analysis of GOT1 using anti-GOT1 antibody (M04085). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human A431 whole cell lysates,<br>
Lane 5: rat heart tissue lysates,<br>
Lane 6: rat liver tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse liver tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOT1 antigen affinity purified monoclonal antibody (M04085) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GOT1 at approximately 41 kDa. The expected band size for GOT1 is at 46 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-claudin-4-antibody-m03683-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03683-wb.jpgAnti-Claudin 4 Monoclonal AntibodyWestern blot analysis of Claudin 4 expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pomc-antibody-m00305-1-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00305-1-wb.jpgAnti-POMC Monoclonal AntibodyWestern blot analysis of POMC expression in mouse pituitary lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-1-antichymotrypsin-antibody-m02312-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02312-wb.jpgAnti-alpha 1 Antichymotrypsin SERPINA3 Monoclonal AntibodyWestern blot analysis of alpha 1 Antichymotrypsin expression in human plasma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gamma-synuclein-antibody-m03523-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03523-wb.jpgAnti-gamma Synuclein Rabbit Monoclonal AntibodyWestern blot analysis of gamma Synuclein expression in human fetal brain lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd97-antibody-m02982-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02982-cd97-primary-antibodies-wb-testing-1.jpgAnti-CD97 Monoclonal AntibodyWestern blot analysis of CD97 using anti-CD97 antibody (M02982). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: human A549 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD97 antigen affinity purified monoclonal antibody (M02982) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD97 at approximately 75 kDa. The expected band size for CD97 is at 92 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sa2-antibody-m03624-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03624-wb.jpgAnti-SA2 Rabbit Monoclonal AntibodyWestern blot analysis of SA2 expression in (1) K562 cell lysate; (2) Raw264.7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bob1-antibody-m04431-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-pou2af1-primary-antibodies-wb-testing-1.jpgAnti-BOB1 Monoclonal Antibody Western blot analysis of POU2AF1 using anti-POU2AF1 antibody (M04431). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU2AF1 antigen affinity purified monoclonal antibody (Catalog # M04431) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POU2AF1 at approximately 37 kDa. The expected band size for POU2AF1 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-pou2af1-primary-antibodies-ihc-testing-2.jpgAnti-BOB1 Monoclonal Antibody IHC analysis of POU2AF1 using anti-POU2AF1 antibody (M04431). <br>
POU2AF1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-POU2AF1 Antibody (M04431) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-pou2af1-primary-antibodies-ihc-testing-3.jpgAnti-BOB1 Monoclonal Antibody IHC analysis of POU2AF1 using anti-POU2AF1 antibody (M04431). <br>
POU2AF1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-POU2AF1 Antibody (M04431) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-pou2af1-primary-antibodies-ihc-testing-4.jpgAnti-BOB1 Monoclonal Antibody IHC analysis of POU2AF1 using anti-POU2AF1 antibody (M04431). <br>
POU2AF1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-POU2AF1 Antibody (M04431) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-pou2af1-primary-antibodies-ihc-testing-5.jpgAnti-BOB1 Monoclonal Antibody IHC analysis of POU2AF1 using anti-POU2AF1 antibody (M04431). <br>
POU2AF1 was detected in a paraffin-embedded section of rat lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-POU2AF1 Antibody (M04431) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mad2l2-antibody-m02357-boster.html2026-03-24T05:19:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02357-wb.jpgAnti-Mad2L2 Monoclonal AntibodyWestern blot analysis of Mad2L2 expression in Hela cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02357-12885_2023_11648_fig8_html.pngAnti-Mad2L2 Monoclonal AntibodyExpression of STAM, ANXA5 and MAD2L2 in HCC cell lines. A The expressions of STAM, ANXA5 and MAD2L2 in normal hepatocytes and hepatoma cell lines were detected using Western blotting. Knocking down STAM, ANXA5 and MAD2L2 inhibited the proliferation and migration of HCC cells. B The knockdown efficiency of STAM, ANXA5 and MAD2L2 was detected using Western Blotting. C Plate cloning experiment showed that the number of cloned cell clusters formed by hepatocellular carcinoma cells after knockdown of STAM, ANXA5 and MAD2L2 was significantly reduced; D The results of CCK8 experiment showed that inhibiting the expression of STAM, ANXA5 and MAD2L2 decreased the proliferative ability of HCC cells; E Scratch assay showed that knockdown of STAM, ANXA5 and MAD2L2 inhibited the migration of hepatocellular carcinoma cells; F Transwell experiment showed that the migration ability of hepatocellular carcinoma cells was weakened after inhibiting the expression of STAM, ANXA5 and MAD2L2 .* P < 0.05, ** P < 0.01, *** P < 0.001, there was no significant difference in ns <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-023-11648-x'>38049741</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pak1-antibody-m00454-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00454-pak1-primary-antibodies-wb-testing-1.jpgAnti-PAK1 Monoclonal AntibodyWestern blot analysis of PAK1 using anti-PAK1 antibody (M00454). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified monoclonal antibody (M00454) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 68 kDa. The expected band size for PAK1 is at 61 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pck2-antibody-m04772-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04772-pck2-primary-antibodies-wb-testing-1.jpgAnti-PCK2 Monoclonal AntibodyWestern blot analysis of PCK2 using anti-PCK2 antibody (M04772). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human HEL whole cell lysates,<br>
Lane 4: human LNCAP whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse spleen tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified monoclonal antibody (M04772) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71 kDa. The expected band size for PCK2 is at 71 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pak3-antibody-m03124-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03124-pak3-primary-antibodies-wb-testing-1.jpgAnti-PAK3 Rabbit Monoclonal AntibodyWestern blot analysis of PAK3 using anti-PAK3 antibody (M03124). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 antigen affinity purified monoclonal antibody (M03124) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK3 at approximately 62 kDa. The expected band size for PAK3 is at 62 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctip1-antibody-m00741-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00741-bcl11a-primary-antibodies-wb-testing-1.jpgAnti-Ctip1 Monoclonal Antibody Western blot analysis of Ctip1 using anti-Ctip1 antibody (M00741). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ctip1 antigen affinity purified monoclonal antibody (Catalog # M00741) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ctip1 at approximately 120 kDa. The expected band size for Ctip1 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00741-wb.jpgAnti-Ctip1 Monoclonal AntibodyWestern blot analysis of Ctip1 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pak2-antibody-m01419-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01419-wb.jpgAnti-PAK2 Monoclonal AntibodyWestern blot analysis of PAK2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-pak4-pak5-pak6-s474-s560-s602-antibody-mp01723-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp01723-wb.jpgAnti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal AntibodyWestern blot analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in U87-MG cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trim25-antibody-m03232-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-wb-testing-1.jpgAnti-TRIM25 Monoclonal Antibody Western blot analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified monoclonal antibody (Catalog # M03232) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM25 at approximately 71 kDa. The expected band size for TRIM25 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-wb7.jpgAnti-TRIM25 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-2.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-3.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-4.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-5.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-6.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03232-trim25-primary-antibodies-ihc-testing-7.jpgAnti-TRIM25 Monoclonal Antibody IHC analysis of TRIM25 using anti-TRIM25 antibody (M03232). <br>
TRIM25 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRIM25 Antibody (M03232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-securin-antibody-m02099-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02099-pttg1-primary-antibodies-wb-testing-1.jpgAnti-Securin Monoclonal Antibody Western blot analysis of PTTG1 using anti-PTTG1 antibody (M02099). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human U20S whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates,<br>
Lane 5: human PC-3 whole cell lysates,<br>
Lane 6: human A549 whole cell lysates,<br>
Lane 7: human SIHA whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTTG1 antigen affinity purified monoclonal antibody (M02099) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTTG1 at approximately 26 kDa. The expected band size for PTTG1 is at 22 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-shc-antibody-m00796-1-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00796-1-shc1-primary-antibodies-wb-testing-1.jpgAnti-SHC Monoclonal AntibodyWestern blot analysis of SHC/SHC1 using anti-SHC/SHC1 antibody (M00796-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human U2OS whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: mouse HEPA1-6 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC/SHC1 antigen affinity purified monoclonal antibody (M00796-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHC/SHC1 at approximately 65-70 kDa. The expected band size for SHC/SHC1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00796-1-shc1-primary-antibodies-ihc-testing-1.jpgAnti-SHC Monoclonal AntibodyIHC analysis of SHC/SHC1 using anti-SHC/SHC1 antibody (M00796-1). <br>SHC/SHC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-SHC/SHC1 Antibody (M00796-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00796-1-shc1-primary-antibodies-ihc-testing-2.jpgAnti-SHC Monoclonal AntibodyIHC analysis of SHC/SHC1 using anti-SHC/SHC1 antibody (M00796-1). <br>SHC/SHC1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-SHC/SHC1 Antibody (M00796-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00796-1-shc1-primary-antibodies-ihc-testing-3.jpgAnti-SHC Monoclonal AntibodyIHC analysis of SHC/SHC1 using anti-SHC/SHC1 antibody (M00796-1). <br>SHC/SHC1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-SHC/SHC1 Antibody (M00796-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grb2-antibody-m00351-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00351-wb.jpgAnti-GRB2 Monoclonal AntibodyWestern blot analysis of GRB2 expression in HEK293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd166-antibody-m01788-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01788-wb.jpgAnti-CD166 Monoclonal AntibodyWestern blot analysis of CD166 expression in Daudi cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galectin-1-antibody-m00470-1-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00470-1-wb.jpgAnti-Galectin 1 LGALS1 Monoclonal AntibodyWestern blot analysis of Galectin 1 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-huntingtin-antibody-m00134-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00134-wb.jpgAnti-Huntingtin Monoclonal AntibodyWestern blot analysis of Huntingtin expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trpm7-antibody-m00789-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00789-wb.jpgAnti-TRPM7 Monoclonal AntibodyWestern blot analysis of TRPM7 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00789-fcell-09-760035-g002.jpgAnti-TRPM7 Monoclonal AntibodyLBQ657 significantly extenuated TGF-β1 induced cardiac fibroblast activation in MEF. (A) mRNA expression of Mmp9 and α -Sma in control MEF, MEF treated with TGF-β1 and MEF treated with LBQ657 + TGF-β1. (B) Protein levels of MMP9 and α-SMA in the rats. (C) The efficiency of the siRNA used to reduce the mRNA expression of TRPM7 in MEF. (D) mRNA expression of Trpm7 , Mmp9 and α -Sma in MEF treated with TGF-β1, MEF transfected with si-TRPM7 and treated with TGF-β1, MEF treated with LBQ657 + TGF-β1. (E) Protein levels of TRPM7, MMP9 and α-SMA in the rats. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. 18S RNA was used as the internal reference gene and β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, and ∗∗ P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.760035/full'>34778271</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00789-fcell-09-760035-g003.jpgAnti-TRPM7 Monoclonal AntibodyLBQ657 protected against fibrosis by blocking TRPM7 channel. (A) The virtual binding mode of NEPi and natural cholesterol hemisuccinateon ligand (as control) on TRPM7. NEPi (red) and cholesterol hemisuccinate (gray) are shown as a stick model. (B) Current amplitude of TRPM7-like current recorded in neonatal rat cardiac fibroblasts in control cell (black), LBQ657-10 μM treated cell (blue) and LBQ657-50 μM treated cell (red). (C) mRNA expression of Mmp9 and α -Sma in MEF treated with TGF-β1, MEF treated with 2-APB + TGF-β1, and MEF treated with LBQ657 + TGF-β1. (D) Protein levels of MMP9 and α-SMA in the rats. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. 18S RNA was used as the internal reference gene and β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, and ∗∗ P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.760035/full'>34778271</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00789-fcell-09-760035-g004.jpgAnti-TRPM7 Monoclonal AntibodyLBQ657 reduced hypoxia-induced necrosis by inhibiting TRPM7-mediated Ca 2+ influx in cardiomyocytes. (A) Comparison of TRPM7 protein levels in MEF and H 9 C 2 cells. (B) Fluorescence intensity of Fluo-4-AM fluorescent dye in 488 nm excitation light of normoxic H 9 C 2 , hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (C) Protein levels of caspase3, cleaved caspase3, LC3B-I and LC3B-II in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (D) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with LBQ657. (E) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in normoxic H 9 C 2 and normoxic H 9 C 2 treated with LBQ657. (F) Protein levels of RIPK1/RIPK3/MLKL/p-MLKL in hypoxic H 9 C 2 and hypoxic H 9 C 2 pre-treated with BAPTA-AM. The data presented are mean ± SD. Each group contained the results of three independent repeated trials. β-actin was used as the internal reference protein for normalization and statistical analysis. ∗∗∗∗ P < 0.0001, ∗∗∗ P < 0.001, ∗∗ P < 0.01, and ∗ P < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.760035/full'>34778271</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd33-antibody-m01508-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01508-wb.jpgAnti-CD33 Monoclonal AntibodyWestern blot analysis of CD33 expression in THP1 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp2-antibody-m00286-3-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00286-3-wb.jpgAnti-MMP2 Monoclonal AntibodyWestern blot analysis of MMP2 expression in L6 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iqgap1-antibody-m01603-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01603-wb.jpgAnti-IQGAP1 Monoclonal AntibodyWestern blot analysis of IQGAP1 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sf2-antibody-m00497-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00497-wb.jpgAnti-SF2 Monoclonal AntibodyWestern blot analysis of SF2 expression in mouse spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nrf1-antibody-m01129-boster.html2026-03-24T05:19:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-wb-testing-1.jpgAnti-NRF1 Rabbit Monoclonal Antibody Western blot analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human MOLT-4 whole cell lysates, <br>
Lane 4: human U251 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat PC-12 whole cell lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRF1 antigen affinity purified monoclonal antibody (M01129) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRF1 at approximately 116 kDa. The expected band size for NRF1 is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-2.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-3.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-4.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-5.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-6.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-7.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-8.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human non-small-cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-9.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-10.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-11.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-nrf1-primary-antibodies-ihc-testing-12.jpgAnti-NRF1 Rabbit Monoclonal Antibody IHC analysis of NRF1 using anti-NRF1 antibody (M01129). <br>
NRF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NRF1 Antibody (M01129) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kmt5a-setd8-pr-set7-antibody-m05349-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05349-wb.jpgAnti-KMT5A / SETD8 / Pr-SET7 Monoclonal AntibodyWestern blot analysis of KMT5A / SETD8 / Pr-SET7 expression in (1) 293T cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wdr5-antibody-m01910-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01910-wdr5-primary-antibodies-wb-testing-1.jpgAnti-WDR5 Monoclonal AntibodyWestern blot analysis of WDR5 using anti-WDR5 antibody (M01910). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human MOLT-4 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR5 antigen affinity purified monoclonal antibody (M01910) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR5 at approximately 37 kDa. The expected band size for WDR5 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01910-wdr5-primary-antibodies-ihc-testing-1.jpgAnti-WDR5 Monoclonal AntibodyIHC analysis of WDR5 using anti-WDR5 antibody (M01910). <br>WDR5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WDR5 Antibody (M01910) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01910-wdr5-primary-antibodies-ihc-testing-2.jpgAnti-WDR5 Monoclonal AntibodyIHC analysis of WDR5 using anti-WDR5 antibody (M01910). <br>WDR5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WDR5 Antibody (M01910) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01910-wdr5-primary-antibodies-ihc-testing-3.jpgAnti-WDR5 Monoclonal AntibodyIHC analysis of WDR5 using anti-WDR5 antibody (M01910). <br>WDR5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WDR5 Antibody (M01910) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01910-wdr5-primary-antibodies-ihc-testing-4.jpgAnti-WDR5 Monoclonal AntibodyIHC analysis of WDR5 using anti-WDR5 antibody (M01910). <br>WDR5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-WDR5 Antibody (M01910) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbx7-antibody-m04742-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04742-wb.jpgAnti-cbx7 Monoclonal AntibodyWestern blot analysis of cbx7 expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mttfa-antibody-m01119-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-wb.jpgAnti-mtTFA Rabbit Monoclonal AntibodyWestern blot analysis of mtTFA expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-anp-antibody-m01318-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01318-wb.jpgAnti-ANP NPPA Monoclonal AntibodyWestern blot analysis of ANP expression in PC3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smyd3-antibody-m02080-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-wb7.jpgAnti-SMYD3 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-icc1.jpgAnti-SMYD3 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-wb8.jpgAnti-SMYD3 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-icc2.jpgAnti-SMYD3 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-wb.jpgAnti-SMYD3 Monoclonal AntibodyWestern blot analysis of SMYD3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02080-icc3.jpgAnti-SMYD3 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-egr1-antibody-m00687-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00687-egr1-primary-antibodies-ihc-testing-1.jpgAnti-Egr1 Monoclonal AntibodyIHC analysis of Egr1 using anti-Egr1 antibody (M00687). <br>Egr1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Egr1 Antibody (M00687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00687-egr1-primary-antibodies-ihc-testing-2.jpgAnti-Egr1 Monoclonal AntibodyIHC analysis of Egr1 using anti-Egr1 antibody (M00687). <br>Egr1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Egr1 Antibody (M00687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00687-egr1-primary-antibodies-wb-testing-1.jpgAnti-Egr1 Monoclonal AntibodyWestern blot analysis of Egr1 using anti-Egr1 antibody (M00687). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Egr1 antigen affinity purified monoclonal antibody (M00687) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Egr1 at approximately 58 kDa. The expected band size for Egr1 is at 58 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dna-ligase-iv-antibody-m01685-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01685-wb7.jpgAnti-DNA Ligase IV Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01685-wb.jpgAnti-DNA Ligase IV Rabbit Monoclonal AntibodyWestern blot analysis of DNA Ligase IV expression in Ramos cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01685-wb8.jpgAnti-DNA Ligase IV Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01685-wb9.jpgAnti-DNA Ligase IV Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rantes-antibody-m00617-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00617-wb.jpgAnti-RANTES CCL5 Monoclonal AntibodyWestern blot analysis of RANTES expression in RANTES recombinant protein lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hur-elavl1-antibody-m00736-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00736-elavl1-primary-antibodies-wb-testing-1.jpgAnti-HuR / ELAVL1 Monoclonal Antibody Western blot analysis of ELAVL1 using anti-ELAVL1 antibody (M00736). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human MOLT-4 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat heart tissue lysates,<br>
Lane 6: rat spleen tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse spleen tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL1 antigen affinity purified monoclonal antibody (Catalog # M00736) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL1 at approximately 36 kDa. The expected band size for ELAVL1 is at 36 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zeb1-antibody-m00548-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-icc1.jpgAnti-ZEB1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-wb.jpgAnti-ZEB1 Monoclonal AntibodyWestern blot analysis of AREB6 expression in HEK293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cullin-1-antibody-m00658-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-wb-testing-1.jpgAnti-Cullin 1 Monoclonal AntibodyWestern blot analysis of CUL1 using anti-CUL1 antibody (M00658). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human SIHA whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: rat RH35 whole cell lysates,<br>
Lane 7: mouse HEPA1-6 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL1 antigen affinity purified monoclonal antibody (M00658) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CUL1 at approximately 85 kDa. The expected band size for CUL1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-1.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-2.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-3.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-4.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-5.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-6.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-7.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-8.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-9.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00658-cul1-primary-antibodies-ihc-testing-10.jpgAnti-Cullin 1 Monoclonal AntibodyIHC analysis of CUL1 using anti-CUL1 antibody (M00658). <br>CUL1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL1 Antibody (M00658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-estrogen-inducible-protein-ps2-antibody-m01391-1-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01391-1-wb.jpgAnti-Estrogen Inducible Protein pS2 Monoclonal AntibodyWestern blot analysis of Estrogen Inducible Protein pS2 expression in MCF-7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd23-antibody-m04237-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04237-wb.jpgAnti-CD23 FCER2 Monoclonal AntibodyWestern blot analysis of CD23 expression in Raji cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm5-antibody-m03642-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03642-wb.jpgAnti-MCM5 Monoclonal AntibodyWestern blot analysis of MCM5 expression in MCF-7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mybbp1a-antibody-m04187-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04187-wb.jpgAnti-MYBBP1A Monoclonal AntibodyWestern blot analysis of MYBBP1A expression in HEK293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lyric-antibody-m04060-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04060-wb.jpgAnti-LYRIC Monoclonal AntibodyWestern blot analysis of LYRIC expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd7-antibody-m01974-boster.html2026-03-24T05:19:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01974-wb.jpgAnti-CD7 Monoclonal AntibodyWestern blot analysis of CD7 expression in human PBMC lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd2-antibody-m01746-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01746-mbd2-primary-antibodies-wb-testing-1.jpgAnti-MBD2 Rabbit Monoclonal AntibodyWestern blot analysis of MBD2 using anti-MBD2 antibody (M01746). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hacat whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: mouse liver tissue lysates,<br>
Lane 7: mouse liver tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD2 antigen affinity purified monoclonal antibody (M01746) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MBD2 at approximately 49 kDa. The expected band size for MBD2 is at 43 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pfkfb3-antibody-m02382-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02382-icc2.jpgAnti-PFKFB3 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02382-pfkfb3-primary-antibodies-wb-testing-1_1.jpgAnti-PFKFB3 Monoclonal AntibodyWestern blot analysis of PFKFB3 using anti-PFKFB3 antibody (M02382). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human U251 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB3 antigen affinity purified monoclonal antibody (M02382) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFKFB3 at approximately 58 kDa. The expected band size for PFKFB3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02382-icc1.jpgAnti-PFKFB3 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ksr1-antibody-m03285-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03285-wb.jpgAnti-KSR1 Rabbit Monoclonal AntibodyWestern blot analysis of KSR1 expression in HEK293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdk2-antibody-m03261-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03261-wb.jpgAnti-PDK2 Monoclonal AntibodyWestern blot analysis of PDK2 expression in (1) SH-SY5Y cell lysate; (2) Mouse heart lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stip1-antibody-m02683-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02683-wb.jpgAnti-STIP1 Monoclonal AntibodyWestern blot analysis of STIP1 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syngap-antibody-m04862-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04862-syngap1-primary-antibodies-wb-testing-1.jpgAnti-SynGAP Rabbit Monoclonal AntibodyWestern blot analysis of SYNGAP1 using anti-SYNGAP1 antibody (M04862). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNGAP1 antigen affinity purified monoclonal antibody (M04862) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYNGAP1 at approximately 148 kDa. The expected band size for SYNGAP1 is at 148 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04862-syngap-primary-antibodies-if-testing-1.jpgAnti-SynGAP Rabbit Monoclonal AntibodyIF analysis of SYNGAP using anti-SYNGAP antibody (M04862).<br>
SYNGAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-SYNGAP Antibody (M04862) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cullin-3-antibody-m00747-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00747-wb.jpgAnti-Cullin 3 Monoclonal AntibodyWestern blot analysis of Cullin 3 expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpa70-antibody-m01317-1-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-1-rpa1-primary-antibodies-wb-testing-1.jpgAnti-RPA70 Monoclonal AntibodyWestern blot analysis of RPA1 using anti-RPA1 antibody (M01317-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human SIHA whole cell lysates,<br>
Lane 4: human Jurkat whole cell lysates,<br>
Lane 5: human A549 whole cell lysates,<br>
Lane 6: human Caco-2 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA1 antigen affinity purified monoclonal antibody (M01317-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPA1 at approximately 68 kDa. The expected band size for RPA1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-1-rpa1-primary-antibodies-ihc-testing-1.jpgAnti-RPA70 Monoclonal AntibodyIHC analysis of RPA1 using anti-RPA1 antibody (M01317-1). <br>RPA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-RPA1 Antibody (M01317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-1-rpa1-primary-antibodies-ihc-testing-2.jpgAnti-RPA70 Monoclonal AntibodyIHC analysis of RPA1 using anti-RPA1 antibody (M01317-1). <br>RPA1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-RPA1 Antibody (M01317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-1-rpa1-primary-antibodies-ihc-testing-3.jpgAnti-RPA70 Monoclonal AntibodyIHC analysis of RPA1 using anti-RPA1 antibody (M01317-1). <br>RPA1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-RPA1 Antibody (M01317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bag2-antibody-m04933-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04933-wb.jpgAnti-BAG2 Monoclonal AntibodyWestern blot analysis of BAG2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-inhibin-alpha-antibody-m02413-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02413-wb.jpgAnti-Inhibin alpha INHA Monoclonal AntibodyWestern blot analysis of Inhibin alpha expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epac1-antibody-m02483-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02483-epac1-primary-antibodies-wb-testing-1.jpgAnti-Epac1 Rabbit Monoclonal Antibody Western blot analysis of Epac1 using anti-Epac1 antibody (M02483). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HUVEC whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: rat kidney tissue lysates,<br>
Lane 4: mouse brain tissue lysates,<br>
Lane 5: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1 antigen affinity purified monoclonal antibody (Catalog # M02483) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epac1 at approximately 104 kDa. The expected band size for Epac1 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02483-rapgef3-primary-antibodies-ihc-testing-2.jpgAnti-Epac1 Rabbit Monoclonal Antibody IHC analysis of Epac1 using anti-Epac1 antibody (M02483). <br>
Epac1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Epac1 Antibody (M02483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02483-rapgef3-primary-antibodies-ihc-testing-3.jpgAnti-Epac1 Rabbit Monoclonal Antibody IHC analysis of Epac1 using anti-Epac1 antibody (M02483). <br>
Epac1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Epac1 Antibody (M02483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gclm-antibody-m02948-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-wb7.jpgAnti-GCLM Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-wb8.jpgAnti-GCLM Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-wb.jpgAnti-GCLM Monoclonal AntibodyWestern blot analysis of GCLM expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgc1-beta-antibody-m02933-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02933-ppargc1b-primary-antibodies-wb-testing-1.jpgAnti-PGC1 beta PPARGC1B Monoclonal Antibody Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (M02933). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified monoclonal antibody (Catalog # M02933) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113 kDa. The expected band size for PPARGC1B is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02933-wb.jpgAnti-PGC1 beta PPARGC1B Monoclonal AntibodyWestern blot analysis of PGC1 beta expression in (1) HeLa cell lysate; (2) Mouse spleen lysate; (3) Rat spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem173-antibody-m01871-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-sting-primary-antibodies-wb-testing-1.jpgAnti-TMEM173 Monoclonal Antibody Western blot analysis of TMEM173 using anti-TMEM173 antibody (M01871). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173 antigen affinity purified monoclonal antibody (Catalog # M01871) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM173 at approximately 37 kDa. The expected band size for TMEM173 is at 37,42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-wb7.jpgAnti-TMEM173 Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fto-antibody-m00219-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-wb-testing-1_1.jpgAnti-FTO Monoclonal AntibodyWestern blot analysis of FTO using anti-FTO antibody (M00219). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human SiHa whole cell lysates,<br>
Lane 4: human Daudi whole cell lysates,<br>
Lane 5: human HepG2 whole cell lysates,<br>
Lane 6: human T-47D whole cell lysates,<br>
Lane 7: rat RH35 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified monoclonal antibody (M00219) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-wb-testing-2.jpgAnti-FTO Monoclonal AntibodyWestern blot analysis of FTO using anti-FTO antibody (M00219).<br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br>
Lane 1: Human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified monoclonal antibody (M00219) at a dilution of 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa.The expected band size for FTO is at 58 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-icc1.jpgAnti-FTO Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-1.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-icc2.jpgAnti-FTO Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-2.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-3.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human esophagus cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-4.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-5.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-6.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-7.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-fto-primary-antibodies-ihc-testing-8.jpgAnti-FTO Monoclonal AntibodyIHC analysis of FTO using anti-FTO antibody (M00219). <br>FTO was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FTO Antibody (M00219) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldolase-antibody-m05022-boster.html2026-03-24T05:19:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05022-wb.jpgAnti-Aldolase ALDOA Monoclonal AntibodyWestern blot analysis of Aldolase expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psd93-antibody-m04826-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04826-psd93-primary-antibodies-wb-testing-1.jpgAnti-PSD93 Monoclonal AntibodyWestern blot analysis of PSD93 using anti-PSD93 antibody (M04826). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human T-47D whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD93 antigen affinity purified monoclonal antibody (M04826) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSD93 at approximately 100 kDa. The expected band size for PSD93 is at 98 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pyruvate-dehydrogenase-e1-beta-subunit-antibody-m03998-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03998-pdhb-primary-antibodies-wb-testing-1.jpgAnti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal AntibodyWestern blot analysis of PDHB using anti-PDHB antibody (M03998). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human Hela whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDHB antigen affinity purified monoclonal antibody (M03998) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDHB at approximately 35 kDa. The expected band size for PDHB is at 35 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rrm2-antibody-m01978-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01978-rrm2-primary-antibodies-wb-testing-1.jpgAnti-RRM2 Monoclonal Antibody Western blot analysis of RRM2 using anti-RRM2 antibody (M01978). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human SIHA whole cell lysates,<br>
Lane 3: human U251 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified monoclonal antibody (Catalog # M01978) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 45 kDa. The expected band size for RRM2 is at 45 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kpna2-antibody-m01776-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01776-wb.jpgAnti-KPNA2 Monoclonal AntibodyWestern blot analysis of KPNA2 expression in (1) HeLa cell lysate; (2) C6 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddah1-antibody-m03136-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03136-ddah1-primary-antibodies-wb-testing-1.jpgAnti-DDAH1 Monoclonal Antibody Western blot analysis of DDAH1 using anti-DDAH1 antibody (M03136). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: monkey COS-7 whole cell lysates,<br>
Lane 4: human SH-SY5Y whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified monoclonal antibody (Catalog # M03136) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 37 kDa. The expected band size for DDAH1 is at 31 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nogo-antibody-m01844-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01844-wb.jpgAnti-Nogo Monoclonal AntibodyWestern blot analysis of Nogo expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sae2-uba2-antibody-m03816-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03816-wb.jpgAnti-SAE2 / UBA2 Monoclonal AntibodyWestern blot analysis of SAE2 / UBA2 expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alix-antibody-m01751-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-alix-primary-antibodies-wb-testing-1.jpgAnti-ALIX Rabbit Monoclonal Antibody Western blot analysis of ALIX using anti-ALIX antibody (M01751). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human Raji whole cell lysates,<br>
Lane 3: human Daudi whole cell lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: rat lung tissue lysates,<br>
Lane 6: mouse liver tissue lysates,<br>
Lane 7: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified monoclonal antibody (Catalog # M01751) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-jev2-14-e70087-g006.jpgAnti-ALIX Rabbit Monoclonal AntibodyA‐C. Prove of urinary extracellular vesicle (uEV) particle existence in the primary analyte samples derived by different uEV isolation methods by ancillary transmission electron microscopy (TEM) (A), fluorescence microscopy (FM) (B) and Western Blotting/Immunoblotting (IB) (C) methods. TEM shows a typical cup‐shape EV morphology (EV drying artefact) at 3200 ms exposure and either medium 15,000× (bar size = 200 nm) or high 43,000× (bar size = 100 nm, inlet image) magnification; IB shows known EV protein biomarker expression such as Alix, TSG101, Flotillin‐1, CD‐9 and PKM1/2 in different subject (S1, S2) uEV samples that are free of Golgi, endoplasmic reticulum or mitochondria organelle contaminants as indicated by the absence of GM130, protein disulfide isomerase (PDI) or pyruvate dehydrogenase (PD) proteins; FM displays green fluorescent signal emitted from SYTO RNASelect‐stained internal uEV nucleic acid (RNA) cargo (bar size = 10 µm). (D) Linear relationship between raw source sample dose (pre‐clarified urine input) and isolated uEV particle concentration (output response) in samples processed by DC (left panel), SiC (middle panel) or PEG (right panel) methods.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12086329/'>40384173</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-jev2-14-e70087-g008.jpgAnti-ALIX Rabbit Monoclonal AntibodyRepeatability of selected urinary extracellular vesicle (uEV) protein content measurements by Multi‐Strip Western Blotting (MSWB). (A) The principle of MSWB analysis. Multiple gels were loaded with molecular weight marker (Mr, lanes 1 and 10) and lysates of either individual technical replicate (TR) samples (lanes 3–8) or pooled master TR7 sample in duplicate (lanes 2 and 9) prepared by mixing equal amounts of TR1–TR6 lysates of either uMV or uEXO fractions to resolve proteins by their molecular weight (left panel). After electrophoresis, these gels were cut into strips. Strips that contained the identical antigen of interest, were aligned onto single assembling filter paper, and then transferred onto single nitrocellulose membrane. Following protein transfer, the membrane was blocked and probed with appropriate primary and HRP‐conjugated secondary antibodies. For visualization, some strips were rearranged to alternate between male ( M ) and female ( F ) subjects and between uEXO and uMV series, because paired samples were loaded in a randomised manner. Black line strokes around the individual strips were drawn to improve readability. The representative blot shows 50 kDa TSG101 protein level distribution across TR1‐TR7 samples of paired uEV fractions of individual subjects (right panel). (B) Comparison of procedural sample processing precision for selected endogenous uEV protein detection following uEV isolation by DC method. Relative uncertainty of chemiluminescent protein band signal measurements was expressed as CV TR to assess average procedural sample processing precision in six uMV ( left panel ) or six uEXO ( right panel ) TR samples obtained from male ( teal bars ) ( N = 3) or female ( pink bars ) urine ( N = 3) by differential velocity centrifugation (DC) method. (C) Comparative uEV‐associated protein marker expression levels before and after elimination of procedural sample processing variations by sample pooling. Average percentage difference calculated between the arithmetic mean of signal detected across six individual TR samples for PKM1/2 ( upside triangle symbols ), GAPDH ( triangle symbols ), TSG101 ( square symbols ) and ALIX ( circle symbols ) protein bands and an average signal of pooled (TR7) sample, which was loaded in duplicate. The analysis was performed on 8 data points (4 uMV and 4 uEXO series).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12086329/'>40384173</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-txnip-antibody-m01409-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01409-txnip-primary-antibodies-wb-testing-1.jpgAnti-TXNIP Monoclonal AntibodyWestern blot analysis of TXNIP using anti-TXNIP antibody (M01409). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SIHA whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: mouse lung tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNIP antigen affinity purified monoclonal antibody (M01409) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TXNIP at approximately 50 kDa. The expected band size for TXNIP is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01409-txnip-primary-antibodies-ihc-testing-2.jpgAnti-TXNIP Monoclonal AntibodyIHC analysis of TXNIP using anti-TXNIP antibody (M01409). <br>TXNIP was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TXNIP Antibody (M01409) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iba1-antibody-m01394-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-aif1-wb-test-1.jpgAnti-Iba1 AIF1 Monoclonal AntibodyWestern blot analysis of Iba1 expression in U937 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-wb7.jpgAnti-Iba1 AIF1 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperaturehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-wb8.jpgAnti-Iba1 AIF1 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-aif1-primary-antibodies-ihc-testing-2.jpgAnti-Iba1 AIF1 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-aif1-primary-antibodies-if-testing-3.jpgAnti-Iba1 AIF1 Monoclonal AntibodyImmunofluorescence staining of paraffin- embedded human spleen tissue using anti-Iba1 antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hausp-usp7-antibody-m01239-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-wb7.jpgAnti-HAUSP / USP7 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-wb8.jpgAnti-HAUSP / USP7 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-wb.jpgAnti-HAUSP / USP7 Rabbit Monoclonal AntibodyWestern blot analysis of HAUSP / USP7 expression in Ramos cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-uspt-primary-antibodies-ihc-testing-1.jpgAnti-HAUSP / USP7 Rabbit Monoclonal AntibodyIHC analysis of USPT using anti-USPT antibody (M01239). <br>USPT was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-USPT Antibody (M01239) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-uspt-primary-antibodies-ihc-testing-2.jpgAnti-HAUSP / USP7 Rabbit Monoclonal AntibodyIHC analysis of USPT using anti-USPT antibody (M01239). <br>USPT was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-USPT Antibody (M01239) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdt2-antibody-m01255-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01255-dtl-primary-antibodies-wb-testing-1.jpgAnti-CDT2 Monoclonal Antibody Western blot analysis of DTL using anti-DTL antibody (M01255). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human SH-SY5Y whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: rat testis tissue lysates, <br>
Lane 5: rat thymus tissue lysates, <br>
Lane 6: mouse testis tissue lysates, <br>
Lane 7: mouse thymus tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTL antigen affinity purified monoclonal antibody (M01255) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DTL at approximately 95 kDa. The expected band size for DTL is at 79 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grk3-antibody-m20057-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/2/m20057-wb.jpgAnti-GRK3 Monoclonal AntibodyWestern blot analysis of GRK3 expression in Ramos cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-numa-antibody-m02018-1-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02018-1-numa1-primary-antibodies-wb-testing-1.jpgAnti-NuMA NUMA1 Monoclonal AntibodyWestern blot analysis of NUMA1 using anti-NUMA1 antibody (M02018-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human Hacat whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse testis tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMA1 antigen affinity purified monoclonal antibody (M02018-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUMA1 at approximately 270 kDa. The expected band size for NUMA1 is at 238 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02018-1-numa-primary-antibodies-ihc-testing-1.jpgAnti-NuMA NUMA1 Monoclonal AntibodyIHC analysis of NUMA using anti-NUMA antibody (M02018-1). <br>NUMA was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NUMA Antibody (M02018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02018-1-numa-primary-antibodies-ihc-testing-2.jpgAnti-NuMA NUMA1 Monoclonal AntibodyIHC analysis of NUMA using anti-NUMA antibody (M02018-1). <br>NUMA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NUMA Antibody (M02018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02018-1-numa-primary-antibodies-ihc-testing-3.jpgAnti-NuMA NUMA1 Monoclonal AntibodyIHC analysis of NUMA using anti-NUMA antibody (M02018-1). <br>NUMA was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NUMA Antibody (M02018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oct-2-antibody-m04407-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-wb.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyWestern blot analysis of Oct-2 expression in Ramos cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-bad-s112-antibody-mp03520-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/p/mp03520-wb.jpgAnti-Phospho-Bad (S112) Monoclonal AntibodyWestern blot analysis of Phospho-Bad (S112) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lman1-antibody-m03628-1-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03628-1-wb.jpgAnti-LMAN1 Monoclonal AntibodyWestern blot analysis of LMAN1 expression in (1) HeLa cell lysate; (2) Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkii-antibody-m03964-2-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03964-2-wb.jpgAnti-CaMKII beta Monoclonal AntibodyWestern blot analysis of CaMKII expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atm-antibody-m00014-1-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00014-1-wb.jpgAnti-ATM Monoclonal AntibodyWestern blot analysis of ATM expression in 293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-liver-carboxylesterase-1-antibody-m01741-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01741-wb.jpgAnti-Liver Carboxylesterase 1 Monoclonal AntibodyWestern blot analysis of Liver Carboxylesterase 1 expression in U937 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nr0b1-dax1-antibody-m01521-boster.html2026-03-24T05:19:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01521-nr0b1dax1-primary-antibodies-wb-testing-1.jpgAnti-NR0B1 / DAX1 Monoclonal Antibody Western blot analysis of NR0B1/DAX1 using anti-NR0B1/DAX1 antibody (M01521). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human RT4 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR0B1/DAX1 antigen affinity purified monoclonal antibody (M01521) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR0B1/DAX1 at approximately 48 kDa. The expected band size for NR0B1/DAX1 is at 48 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-presenilin-1-antibody-m00138-1-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00138-1-wb.jpgAnti-Presenilin 1 Monoclonal AntibodyWestern blot analysis of Presenilin 1 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00138-1-presenilin-1-primary-antibodies-ihc-testing-1.jpgAnti-Presenilin 1 Monoclonal AntibodyIHC analysis of Presenilin 1 using anti-Presenilin 1 antibody (M00138-1). <br>
Presenilin 1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Presenilin 1 Antibody (M00138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00138-1-presenilin-1-primary-antibodies-ihc-testing-2.jpgAnti-Presenilin 1 Monoclonal AntibodyIHC analysis of Presenilin 1 using anti-Presenilin 1 antibody (M00138-1). <br>
Presenilin 1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Presenilin 1 Antibody (M00138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-5-lipoxygenase-antibody-m00847-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00847-alox5-primary-antibodies-wb-testing-1.jpgAnti-5 Lipoxygenase ALOX5 Monoclonal Antibody Western blot analysis of ALOX5 using anti-ALOX5 antibody (M00847). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX5 antigen affinity purified monoclonal antibody (Catalog # M00847) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX5 at approximately 78 kDa. The expected band size for ALOX5 is at 78 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smurf-2-antibody-m02585-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02585-wb7.jpgAnti-SMURF 2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02585-wb8.jpgAnti-SMURF 2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02585-wb.jpgAnti-SMURF 2 Rabbit Monoclonal AntibodyWestern blot analysis of SMURF 2 expression in SH-SY-5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-internexin-antibody-m03756-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03756-wb.jpgAnti-alpha Internexin INA Monoclonal AntibodyWestern blot analysis of alpha Internexin expression in SHSY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps35-antibody-m01644-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01644-wb.jpgAnti-VPS35 Monoclonal AntibodyWestern blot analysis of VPS35 expression in (1) HeLa cell lysate; (2) Mouse kidney lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sfpq-antibody-m02243-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02243-wb.jpgAnti-SFPQ Monoclonal AntibodyWestern blot analysis of SFPQ expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igfbp7-antibody-m02410-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02410-wb7.jpgAnti-IGFBP7 Monoclonal AntibodyAll lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02410-wb.jpgAnti-IGFBP7 Monoclonal AntibodyWestern blot analysis of IGFBP7 expression in SW480 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02410-igfbp7-primary-antibodies-ihc-testing-1.jpgAnti-IGFBP7 Monoclonal AntibodyIHC analysis of IGFBP7 using anti-IGFBP7 antibody (M02410). <br>IGFBP7 was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IGFBP7 Antibody (M02410) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uteroglobin-antibody-m02201-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02201-wb7.jpgAnti-Uteroglobin SCGB1A1 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02201-wb.jpgAnti-Uteroglobin SCGB1A1 Monoclonal AntibodyWestern blot analysis of Uteroglobin expression in A549 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02201-wb8.jpgAnti-Uteroglobin SCGB1A1 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02201-wb9.jpgAnti-Uteroglobin SCGB1A1 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-imp3-antibody-m02362-1-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02362-1-igf2bp3-primary-antibodies-wb-testing-1.jpgAnti-IMP3 IGF2BP3 Monoclonal Antibody Western blot analysis of IGF2BP3 using anti-IGF2BP3 antibody (M02362-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2BP3 antigen affinity purified monoclonal antibody (Catalog # M02362-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2BP3 at approximately 72 kDa. The expected band size for IGF2BP3 is at 64 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ring2-ring1b-rnf2-antibody-m01209-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01209-wb.jpgAnti-RING2 / RING1B / RNF2 Rabbit Monoclonal AntibodyWestern blot analysis of RING2 / RING1B / RNF2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnrnp-u-antibody-m03691-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-wb.jpgAnti-hnRNP U Monoclonal AntibodyWestern blot analysis of hnRNP U expression in K562 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-1.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-2.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-3.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-4.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-5.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-6.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human tonsil cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-7.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-8.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-9.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-10.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-11.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-12.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-13.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03691-hnrnpu-primary-antibodies-ihc-testing-14.jpgAnti-hnRNP U Monoclonal AntibodyIHC analysis of hnRNPU using anti-hnRNPU antibody (M03691). <br>hnRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-hnRNPU Antibody (M03691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-par6-antibody-m04207-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04207-wb.jpgAnti-PAR6 Monoclonal AntibodyWestern blot analysis of PAR6 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h1-2-antibody-m06243-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-1.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-2.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-3.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-4.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-5.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-6.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-ihc-testing-7.jpgAnti-Histone H1.2 Monoclonal AntibodyIHC analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Histone H1.2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H1.2 Antibody (M06243) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06243-histone-h1-2-primary-antibodies-wb-testing-1.jpgAnti-Histone H1.2 Monoclonal AntibodyWestern blot analysis of Histone H1.2 using anti-Histone H1.2 antibody (M06243). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human A375 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H1.2 antigen affinity purified monoclonal antibody (M06243) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H1.2 at approximately 29 kDa. The expected band size for Histone H1.2 is at 29 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ogt-antibody-m00474-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00474-ogt-primary-antibodies-wb-testing-1.jpgAnti-OGT Rabbit Monoclonal AntibodyWestern blot analysis of OGT using anti-OGT antibody (M00474). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: rat spleen tissue lysates,<br>
Lane 7: mouse thymus tissue lysates,<br>
Lane 8: mouse spleen tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified monoclonal antibody (M00474) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OGT at approximately 110 kDa. The expected band size for OGT is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00474-icc2.jpgAnti-OGT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00474-icc1.jpgAnti-OGT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nprc-antibody-m03589-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03589-nprc-primary-antibodies-wb-testing-1.jpgAnti-NPRC Rabbit Monoclonal AntibodyWestern blot analysis of NPRC using anti-NPRC antibody (M03589). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: rat kidney tissue lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: mouse kidney tissue lysates,<br>
Lane 6: mouse heart tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPRC antigen affinity purified monoclonal antibody (M03589) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPRC at approximately 72 kDa. The expected band size for NPRC is at 70 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-claudin-11-antibody-m08381-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08381-wb.jpgAnti-Claudin 11 CLDN11 Monoclonal AntibodyWestern blot analysis of Oligodendrocyte Specific Protein expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-set-antibody-m02211-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02211-wb.jpgAnti-SET Monoclonal AntibodyWestern blot analysis of SET expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-samhd1-antibody-m00592-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00592-wb.jpgAnti-SAMHD1 Monoclonal AntibodyWestern blot analysis of SAMHD1 expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trp1-antibody-m01959-1-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01959-1-wb.jpgAnti-TRP1 Monoclonal AntibodyWestern blot analysis of TRP1 expression in Human melanoma lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sec23-antibody-m05287-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05287-wb.jpgAnti-SEC23 Monoclonal AntibodyWestern blot analysis of SEC23 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wnt2b-antibody-m04879-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04879-wnt2b-primary-antibodies-wb-testing-1.jpgAnti-Wnt2b Monoclonal AntibodyWestern blot analysis of Wnt2b using anti-Wnt2b antibody (M04879). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human U2OS whole cell lysates,<br>
Lane 3: rat testis tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified monoclonal antibody (M04879) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 44 kDa. The expected band size for Wnt2b is at 44 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps26-antibody-m03951-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03951-wb.jpgAnti-VPS26 VPS26A Monoclonal AntibodyWestern blot analysis of VPS26 expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbpjk-antibody-m00767-boster.html2026-03-24T05:19:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00767-wb.jpgAnti-RBPJK Monoclonal AntibodyWestern blot analysis of RBPJK expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jab1-antibody-m01849-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01849-jab1-primary-antibodies-wb-testing-1.jpgAnti-JAB1 Monoclonal AntibodyWestern blot analysis of JAB1 using anti-JAB1 antibody (M01849). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human U-87MG whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat spleen tissue lysates,<br>
Lane 6: rat lung tissue lysates,<br>
Lane 7: mouse spleen tissue lysates,<br>
Lane 8: mouse lung tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAB1 antigen affinity purified monoclonal antibody (M01849) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JAB1 at approximately 35 kDa. The expected band size for JAB1 is at 38 kDahttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01849-cops5-primary-antibodies-ihc-testing-5.jpgAnti-JAB1 Monoclonal AntibodyFigure 5. IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (M01849). <br>JAB1/COPS5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-JAB1/COPS5 Antibody (M01849) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rna-helicase-a-antibody-m02550-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02550-dhx9-primary-antibodies-wb-testing-1.jpgAnti-RNA Helicase A Rabbit Monoclonal Antibody Western blot analysis of RNA Helicase A using anti-RNA Helicase A antibody (M02550). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: mouse testis tissue lysates,<br>
Lane 5: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNA Helicase A antigen affinity purified monoclonal antibody (Catalog # M02550) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNA Helicase A at approximately 141 kDa. The expected band size for RNA Helicase A is at 141 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trbp-antibody-m01680-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01680-wb.jpgAnti-TRBP Monoclonal AntibodyWestern blot analysis of TRBP expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psb9-lmp2-antibody-m02867-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02867-psb9-primary-antibodies-wb-testing-1.jpgAnti-PSB9 / LMP2 Rabbit Monoclonal AntibodyWestern blot analysis of PSB9 using anti-PSB9 antibody (M02867). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human A431 whole cell lysates,<br>
Lane 2: human HL-60 whole cell lysates,<br>
Lane 3: human Raji whole cell lysates,<br>
Lane 4: human HEL whole cell lysates,<br>
Lane 5: rat liver tissue lysates,<br>
Lane 6: rat spleen tissue lysates,<br>
Lane 7: mouse liver tissue lysates,<br>
Lane 8: mouse spleen tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSB9 antigen affinity purified monoclonal antibody (M02867) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSB9 at approximately 21 kDa. The expected band size for PSB9 is at 21 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikaros-antibody-m00531-1-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00531-1-wb.jpgAnti-Ikaros Monoclonal AntibodyWestern blot analysis of Ikaros expression in MOLT4 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cralbp-antibody-m05421-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05421-wb.jpgAnti-CRALBP RLBP1 Monoclonal AntibodyWestern blot analysis of CRALBP expression in mouse eyeball lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dcp1a-antibody-m04587-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04587-wb.jpgAnti-DCP1A Monoclonal AntibodyWestern blot analysis of DCP1A expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crm1-antibody-m01180-1-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01180-1-wb7.jpgAnti-CRM1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01180-1-wb.jpgAnti-CRM1 Rabbit Monoclonal AntibodyWestern blot analysis of CRM1 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nesprin-1-antibody-m02192-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-syne1-primary-antibodies-wb-testing-1.jpgAnti-Nesprin 1 Rabbit Monoclonal Antibody Western blot analysis of SYNE1 using anti-SYNE1 antibody (M02192). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human U20S whole cell lysates,<br>
Lane 5: human PC12 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNE1 antigen affinity purified monoclonal antibody (M02192) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYNE1 at approximately 131 kDa. The expected band size for SYNE1 is at 1011 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-wb8.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-wb.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyWestern blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc1.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc2.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc3.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc4.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc5.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02192-icc6.jpgAnti-Nesprin 1 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgdh-antibody-m02686-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02686-wb.jpgAnti-PGDH Rabbit Monoclonal AntibodyWestern blot analysis of Prostaglandin dehydrogenase 1 expression in SW480 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phox2b-antibody-m02221-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02221-wb.jpgAnti-PHOX2B Monoclonal AntibodyWestern blot analysis of PHOX2B expression in Neuro-2a cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytochrome-p450-reductase-antibody-m02166-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02166-wb.jpgAnti-Cytochrome P450 Reductase Monoclonal AntibodyWestern blot analysis of Cytochrome P450 Reductase expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmb8-lmp7-antibody-m02188-2-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02188-2-wb.jpgAnti-PSMB8 / LMP7 Monoclonal AntibodyWestern blot analysis of Proteasome 20S LMP7 expression in U937 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02188-2-wb9.jpgAnti-PSMB8 / LMP7 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02188-2-wb7.jpgAnti-PSMB8 / LMP7 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02188-2-wb8.jpgAnti-PSMB8 / LMP7 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-geminin-antibody-m06060-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06060-wb.jpgAnti-Geminin Monoclonal AntibodyWestern blot analysis of Geminin expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-furin-antibody-m01344-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01344-wb.jpgAnti-Furin Monoclonal AntibodyWestern blot analysis of Furin expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alkaline-phosphatase-antibody-m06115-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06115-wb.jpgAnti-Alkaline phosphatase ALPI Rabbit Monoclonal AntibodyWestern blot analysis of intestinal alkaline phosphatase expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thioredoxin-2-antibody-m04586-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-trx2-primary-antibodies-ihc-testing-1.jpgAnti-Thioredoxin 2 Monoclonal AntibodyIHC analysis of TRX2 using anti-TRX2 antibody (M04586). <br>TRX2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRX2 Antibody (M04586) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-trx2-primary-antibodies-ihc-testing-2.jpgAnti-Thioredoxin 2 Monoclonal AntibodyIHC analysis of TRX2 using anti-TRX2 antibody (M04586). <br>TRX2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRX2 Antibody (M04586) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-trx2-primary-antibodies-ihc-testing-3.jpgAnti-Thioredoxin 2 Monoclonal AntibodyIHC analysis of TRX2 using anti-TRX2 antibody (M04586). <br>TRX2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRX2 Antibody (M04586) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-trx2-primary-antibodies-wb-testing-1.jpgAnti-Thioredoxin 2 Monoclonal AntibodyWestern blot analysis of TRX2 using anti-TRX2 antibody (M04586). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Raji whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human HEL whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat skeletal muscle tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse skeletal muscle tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRX2 antigen affinity purified monoclonal antibody (M04586) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRX2 at approximately 14 kDa. The expected band size for TRX2 is at 14 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calsequestrin-1-antibody-m05235-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05235-casq1-primary-antibodies-ihc-testing-1.jpgAnti-Calsequestrin 1 Monoclonal AntibodyIHC analysis of CASQ1 using anti-CASQ1 antibody (M05235). <br>CASQ1 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CASQ1 Antibody (M05235) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05235-casq1-primary-antibodies-ihc-testing-2.jpgAnti-Calsequestrin 1 Monoclonal AntibodyIHC analysis of CASQ1 using anti-CASQ1 antibody (M05235). <br>CASQ1 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CASQ1 Antibody (M05235) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05235-casq1-primary-antibodies-wb-testing-1.jpgAnti-Calsequestrin 1 Monoclonal AntibodyWestern blot analysis of CASQ1 using anti-CASQ1 antibody (M05235). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat skeletal muscle tissue lysates,<br>
Lane 2: mouse skeletal muscle tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASQ1 antigen affinity purified monoclonal antibody (M05235) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CASQ1 at approximately 63 kDa. The expected band size for CASQ1 is at 63 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tgf-alpha-antibody-m01779-1-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01779-1-tgfa-primary-antibodies-wb-testing-1.jpgAnti-TGF alpha Monoclonal Antibody Western blot analysis of TGFA using anti-TGFA antibody (M01779-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A431 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFA antigen affinity purified monoclonal antibody (Catalog # M01779-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFA at approximately 17 kDa. The expected band size for TGFA is at 17 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddah2-antibody-m07566-boster.html2026-03-24T05:19:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07566-wb.jpgAnti-DDAH2 Monoclonal AntibodyWestern blot analysis of DDAH2 expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trap-antibody-m03277-1-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03277-1-acp5-primary-antibodies-wb-testing-1.jpgAnti-TRAP ACP5 Monoclonal Antibody Western blot analysis of ACP5 using anti-ACP5 antibody (M03277-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP5 antigen affinity purified monoclonal antibody (Catalog # M03277-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACP5 at approximately 37 kDa. The expected band size for ACP5 is at 37 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hes5-antibody-m05384-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05384-hes5-primary-antibodies-wb-testing-1.jpgAnti-HES5 Monoclonal Antibody Western blot analysis of HES5 using anti-HES5 antibody (M05384). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: human RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HES5 antigen affinity purified monoclonal antibody (M05384) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HES5 at approximately 18 kDa. The expected band size for HES5 is at 18 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-parathyroid-hormone-receptor-1-antibody-m01588-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01588-pth1r-primary-antibodies-wb-testing-1.jpgAnti-Parathyroid Hormone Receptor 1 Monoclonal AntibodyWestern blot analysis of PTH1R using anti-PTH1R antibody (M01588). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human SIHA whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH1R antigen affinity purified monoclonal antibody (M01588) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTH1R at approximately 66 kDa. The expected band size for PTH1R is at 66 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd134-antibody-m02495-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02495-wb.jpgAnti-CD134 Monoclonal AntibodyWestern blot analysis of CD134 expression in Human fetal spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm4a-antibody-m02850-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02850-wb7.jpgAnti-KDM4A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02850-wb.jpgAnti-KDM4A Monoclonal AntibodyWestern blot analysis of KDM4A / JHDM3A / JMJD2A expression in (1) HEK293 cell lysate; (2) NIH3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm5a-antibody-m03079-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03079-wb.jpgAnti-KDM5A Monoclonal AntibodyWestern blot analysis of KDM5A / Jarid1A / RBBP2 expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kat13a-src1-antibody-m00856-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00856-ncoa1-primary-antibodies-wb-testing-1.jpgAnti-KAT13A / SRC1 Monoclonal AntibodyWestern blot analysis of NCOA1 using anti-NCOA1 antibody (M00856). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human RT4 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA1 antigen affinity purified monoclonal antibody (M00856) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCOA1 at approximately 157 kDa. The expected band size for NCOA1 is at 157 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00856-ncoa1-primary-antibodies-ihc-testing-1.jpgAnti-KAT13A / SRC1 Monoclonal AntibodyIHC analysis of NCOA1 using anti-NCOA1 antibody (M00856). <br>NCOA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-NCOA1 Antibody (M00856) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00856-ncoa1-primary-antibodies-ihc-testing-2.jpgAnti-KAT13A / SRC1 Monoclonal AntibodyIHC analysis of NCOA1 using anti-NCOA1 antibody (M00856). <br>NCOA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-NCOA1 Antibody (M00856) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00856-icc2.jpgAnti-KAT13A / SRC1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00856-icc1.jpgAnti-KAT13A / SRC1 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gelsolin-antibody-m01598-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01598-wb.jpgAnti-Gelsolin GSN Rabbit Monoclonal AntibodyWestern blot analysis of Gelsolin expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd162-antibody-m03674-1-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03674-1-wb.jpgAnti-CD162 Monoclonal AntibodyWestern blot analysis of CD162 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-casein-kinase-2-beta-antibody-m03475-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03475-csnk2b-primary-antibodies-wb-testing-1.jpgAnti-Casein Kinase 2 beta Monoclonal AntibodyFigure 1. Western blot analysis of CSNK2B using anti-CSNK2B antibody (M03475). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse Nerro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2B antigen affinity purified monoclonal antibody (M03475) at a dilution of 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CSNK2B at approximately 25 kDa. The expected band size for CSNK2B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03475-csnk2b-primary-antibodies-ihc-testing-2.jpgAnti-Casein Kinase 2 beta Monoclonal AntibodyFigure 2. IHC analysis of CSNK2B using anti-CSNK2B antibody (M03475). <br>CSNK2B was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-CSNK2B Antibody (M03475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03475-csnk2b-primary-antibodies-ihc-testing-3.jpgAnti-Casein Kinase 2 beta Monoclonal AntibodyFigure 3. IHC analysis of CSNK2B using anti-CSNK2B antibody (M03475). <br>CSNK2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-CSNK2B Antibody (M03475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03475-csnk2b-primary-antibodies-ihc-testing-4.jpgAnti-Casein Kinase 2 beta Monoclonal AntibodyFigure 4. IHC analysis of CSNK2B using anti-CSNK2B antibody (M03475). <br>CSNK2B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-CSNK2B Antibody (M03475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03475-csnk2b-primary-antibodies-ihc-testing-5.jpgAnti-Casein Kinase 2 beta Monoclonal AntibodyFigure 5. IHC analysis of CSNK2B using anti-CSNK2B antibody (M03475). <br>CSNK2B was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-CSNK2B Antibody (M03475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-retinoic-acid-receptor-beta-antibody-m01912-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01912-wb.jpgAnti-Retinoic Acid Receptor beta RARB Monoclonal AntibodyWestern blot analysis of Retinoic Acid Receptor beta expression in MCF7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01912-wb7.jpgAnti-Retinoic Acid Receptor beta RARB Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mesothelin-antibody-m03266-1-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-1-wb7.jpgAnti-Mesothelin Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-1-wb.jpgAnti-Mesothelin Monoclonal AntibodyWestern blot analysis of Mesothelin expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apolipoprotein-a-ii-antibody-m02088-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02088-wb.jpgAnti-Apolipoprotein A II APOA2 Monoclonal AntibodyWestern blot analysis of Apolipoprotein A II expression in Human plasma lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02088-fnut-12-1584551-g008.jpgAnti-Apolipoprotein A II APOA2 Monoclonal AntibodyExpression analyses of transcription or protein levels of selected genes. (A) Expression analyses of selected genes by qRT–PCR. The data represent the means ± SDs from six biological replicates with three technical replicates. (B–D) FCJ/RCJ upregulated ABCC3, IDI1, and APOA2 expression in the liver. FCJ and RCJ significantly upregulated hepatic ABCC3 (B) and IDI1 (C) expression, and RCJ significantly upregulated hepatic APOA2 (D) expression in T2DM rats. The data represent the means ± SDs from three biological replicates with three technical replicates. * p < 0.05, *** p < 0.001, **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1584551/full'>40458826</a></b>
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-biglycan-antibody-m02090-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02090-wb.jpgAnti-Biglycan Monoclonal AntibodyWestern blot analysis of Biglycan expression in C2C12 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-complement-c3-antibody-m00168-2-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00168-2-wb.jpgAnti-Complement C3 Monoclonal AntibodyWestern blot analysis of C3 / C3a expression in Human plasma lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00168-2-wb7.jpgAnti-Complement C3 Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nm23a-antibody-m01334-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01334-wb.jpgAnti-NM23A Monoclonal AntibodyWestern blot analysis of NM23A expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trib3-antibody-m01414-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01414-trib3-primary-antibodies-wb-testing-1.jpgAnti-TRIB3 Monoclonal Antibody Western blot analysis of TRIB3 using anti-TRIB3 antibody (M01414). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB3 antigen affinity purified monoclonal antibody (Catalog # M01414) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIB3 at approximately 40 kDa. The expected band size for TRIB3 is at 40 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd52-antibody-m03484-boster.html2026-03-24T05:19:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03484-wb.jpgAnti-CD52 Monoclonal AntibodyWestern blot analysis of CD52 expression in Mouse spleen cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spi1-antibody-m01116-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01116-spi1-primary-antibodies-wb-testing-1.jpgAnti-SPI1 Rabbit Monoclonal AntibodyWestern blot analysis of SPI1 using anti-SPI1 antibody (M01116). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human HEL whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPI1 antigen affinity purified monoclonal antibody (M01116) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPI1 at approximately 40 kDa. The expected band size for SPI1 is at 31 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cullin4a-antibody-m01579-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01579-cul4a-primary-antibodies-wb-testing-1.jpgAnti-Cullin4A Monoclonal Antibody Western blot analysis of Cullin4A using anti-Cullin4A antibody (M01579). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin4A antigen affinity purified monoclonal antibody (Catalog # M01579) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin4A at approximately 88 kDa. The expected band size for Cullin4A is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01579-cul4a-primary-antibodies-ihc-testing-2.jpgAnti-Cullin4A Monoclonal Antibody IHC analysis of CUL4A using anti-CUL4A antibody (M01579). <br>
CUL4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL4A Antibody (M01579) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01579-cul4a-primary-antibodies-ihc-testing-3.jpgAnti-Cullin4A Monoclonal Antibody IHC analysis of CUL4A using anti-CUL4A antibody (M01579). <br>
CUL4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL4A Antibody (M01579) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbap48-antibody-m02702-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-wb-testing-1.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyWestern blot analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human U20S whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified monoclonal antibody (M02702) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 55 kDa. The expected band size for RbAp48 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-1.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-2.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-3.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-4.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-5.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-6.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-7.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-8.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-9.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-10.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-11.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-12.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-rbbp4-primary-antibodies-ihc-testing-13.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyIHC analysis of RbAp48 using anti-RbAp48 antibody (M02702). <br>RbAp48 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RbAp48 Antibody (M02702) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00996-map2k2-primary-antibodies-ip-testing-1_1.jpgAnti-RbAp48 Rabbit Monoclonal AntibodyImmunoprecipitating (IP) RbAp48 in K562 whole cell lysate.<br>
Western blot analysis of RbAp48 using anti-RbAp48 antibody (M02702); <br>
Lane 1: K562 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-RbAp48 antibody in K562 whole cell lysate;<br>
Lane 3: anti-RbAp48 antibody (2μg) + K562 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RbAp48 antigen affinity purified monoclonal antibody (M02702) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for RbAp48 at approximately 55 kDa. The expected band size for RbAp48 is at 48 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plexin-a1-antibody-m06377-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06377-wb.jpgAnti-Plexin A1 Monoclonal AntibodyWestern blot analysis of Plexin A1 expression in HUVEC cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-villin-antibody-m04205-1-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04205-1-wb.jpgAnti-Villin VIL1 Monoclonal AntibodyWestern blot analysis of Villin expression in Caco-2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trf2-antibody-m00650-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00650-wb7.jpgAnti-TRF2 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00650-icc1.jpgAnti-TRF2 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00650-wb.jpgAnti-TRF2 Monoclonal AntibodyWestern blot analysis of TRF2 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-creatine-kinase-b-type-antibody-m01695-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01695-wb.jpgAnti-Creatine kinase B type CKB Monoclonal AntibodyWestern blot analysis of Creatine kinase B type expression in SHSY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd48-antibody-m03281-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03281-wb.jpgAnti-CD48 Monoclonal AntibodyWestern blot analysis of CD48 expression in Raji cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glycophorin-c-antibody-m05974-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05974-wb.jpgAnti-Glycophorin C GYPC Monoclonal AntibodyWestern blot analysis of Glycophorin C expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-heparanase-1-antibody-m01313-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01313-wb.jpgAnti-Heparanase 1 HPSE Monoclonal AntibodyWestern blot analysis of Heparanase 1 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zyxin-antibody-m02365-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02365-zyx-primary-antibodies-wb-testing-1.jpgAnti-Zyxin Monoclonal Antibody Western blot analysis of Zyxin using anti-Zyxin antibody (M02365). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human SIHA whole cell lysates,<br>
Lane 4: rat lung tissue lysates,<br>
Lane 5: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zyxin antigen affinity purified monoclonal antibody (Catalog # M02365) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zyxin at approximately 78 kDa. The expected band size for Zyxin is at 61 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sprouty-2-antibody-m02089-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02089-wb7.jpgAnti-Sprouty 2 SPRY2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02089-wb10.jpgAnti-Sprouty 2 SPRY2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02089-wb8.jpgAnti-Sprouty 2 SPRY2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02089-wb.jpgAnti-Sprouty 2 SPRY2 Monoclonal AntibodyWestern blot analysis of Sprouty 2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02089-wb9.jpgAnti-Sprouty 2 SPRY2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh2-antibody-m00546-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-wb7.jpgAnti-ALDH2 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-aldh2-primary-antibodies-wb-testing-1.jpgAnti-ALDH2 Monoclonal Antibody Western blot analysis of CDH2 using anti-CDH2 antibody (M01577-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse heart tissue lysates,<br>
Lane 7: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified monoclonal antibody (Catalog # M01577-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140 kDa. The expected band size for CDH2 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-icc1.jpgAnti-ALDH2 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-icc2.jpgAnti-ALDH2 Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikbke-antibody-m01816-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01816-ikbke-primary-antibodies-wb-testing-1.jpgAnti-IKBKE Monoclonal AntibodyWestern blot analysis of IKBKE using anti-IKBKE antibody (M01816). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Raji whole cell lysates,<br>
Lane 2: human Daudi whole cell lysates,<br>
Lane 3: human Ramos whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: human THP-1 whole cell lysates,<br>
Lane 6: human RT4 whole cell lysates,<br>
Lane 7: mouse thymus tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKE antigen affinity purified monoclonal antibody (M01816) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKBKE at approximately 80 kDa. The expected band size for IKBKE is at 80 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cathepsin-s-antibody-m02514-boster.html2026-03-24T05:19:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02514-wb.jpgAnti-Cathepsin S CTSS Monoclonal AntibodyWestern blot analysis of Cathepsin S expression in U87-MG cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ly75-antibody-m08183-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08183-wb.jpgAnti-LY75 Monoclonal AntibodyWestern blot analysis of LY75 expression in Human tonsil lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myh11-antibody-m02422-2-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02422-2-wb.jpgAnti-MYH11 Rabbit Monoclonal AntibodyWestern blot analysis of Myosin, smooth muscle heavy chain 1 and 2 expression in Human bladder lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-netrin-1-antibody-m04892-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04892-wb7.jpgAnti-Netrin 1 NTN1 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04892-wb.jpgAnti-Netrin 1 NTN1 Monoclonal AntibodyWestern blot analysis of Netrin 1 expression in Human fetal brain lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psma1-antibody-m07591-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07591-wb.jpgAnti-PSMA1 Monoclonal AntibodyWestern blot analysis of Proteasome 20S C2 expression in PC3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pmp70-antibody-m02370-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02370-wb7.jpgAnti-PMP70 ABCD3 Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02370-wb.jpgAnti-PMP70 ABCD3 Monoclonal AntibodyWestern blot analysis of PMP70 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02370-wb8.jpgAnti-PMP70 ABCD3 Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02370-wb9.jpgAnti-PMP70 ABCD3 Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bdkrb2-antibody-m03117-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03117-bdkrb2-primary-antibodies-wb-testing-1.jpgAnti-BDKRB2 Monoclonal Antibody Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (M03117). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: mouse lung tissue lysates,<br>
Lane 6: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified monoclonal antibody (Catalog # M03117) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDKRB2 at approximately 78 kDa. The expected band size for BDKRB2 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03117-wb7.jpgAnti-BDKRB2 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03117-bdkrb2-primary-antibodies-ihc-testing-2.jpgAnti-BDKRB2 Monoclonal Antibody IHC analysis of BDKRB2 using anti-BDKRB2 antibody (M03117). <br>
BDKRB2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-BDKRB2 Antibody (M03117) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03117-bdkrb2-primary-antibodies-ihc-testing-3.jpgAnti-BDKRB2 Monoclonal Antibody IHC analysis of BDKRB2 using anti-BDKRB2 antibody (M03117). <br>
BDKRB2 was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-BDKRB2 Antibody (M03117) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddb1-antibody-m00333-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00333-wb.jpgAnti-DDB1 Monoclonal AntibodyWestern blot analysis of DDB1 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytochrome-p450-1a2-antibody-m00598-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00598-wb7.jpgAnti-Cytochrome P450 1A2 CYP1A2 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00598-wb8.jpgAnti-Cytochrome P450 1A2 CYP1A2 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00598-wb.jpgAnti-Cytochrome P450 1A2 CYP1A2 Monoclonal AntibodyWestern blot analysis of Cytochrome P450 1A2 expression in Caco2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ighd-antibody-m05183-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05183-wb.jpgAnti-IGHD Monoclonal AntibodyWestern blot analysis of IGHD expression in Humantonsil cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mst1-antibody-m01069-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01069-wb.jpgAnti-MST1 Rabbit Monoclonal AntibodyWestern blot analysis of MST1 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01069-mst1-primary-antibodies-ihc-testing-1.jpgAnti-MST1 Rabbit Monoclonal AntibodyIHC analysis of MST1 using anti-MST1 antibody (M01069). <br>MST1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MST1 Antibody (M01069) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-napsin-a-antibody-m05685-2-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05685-2-wb.jpgAnti-Napsin A NAPSA Monoclonal AntibodyWestern blot analysis of NAPSIN A expression in Human lung adenocarcinoma lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05685-2-napsa-primary-antibodies-ihc-testing-1.jpgAnti-Napsin A NAPSA Monoclonal AntibodyIHC analysis of NAPSA using anti-NAPSA antibody (M05685-2). <br>NAPSA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NAPSA Antibody (M05685-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tim1-antibody-m01306-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01306-wb.jpgAnti-TIM1 HAVCR1 Monoclonal AntibodyWestern blot analysis of TIM 1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd2-antibody-m00570-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00570-wb.jpgAnti-CD2 Monoclonal AntibodyWestern blot analysis of CD2 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fkbp51-antibody-m04182-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04182-fkbp5-primary-antibodies-wb-testing-1.jpgAnti-FKBP51 Monoclonal Antibody Western blot analysis of FKBP5 using anti-FKBP5 antibody (M04182). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP5 antigen affinity purified monoclonal antibody (Catalog # M04182) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP5 at approximately 51 kDa. The expected band size for FKBP5 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04182-fkbp5-primary-antibodies-ihc-testing-2.jpgAnti-FKBP51 Monoclonal Antibody IHC analysis of FKBP5 using anti-FKBP5 antibody (M04182). <br>
FKBP5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FKBP5 Antibody (M04182) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04182-fkbp5-primary-antibodies-ihc-testing-3.jpgAnti-FKBP51 Monoclonal Antibody IHC analysis of FKBP5 using anti-FKBP5 antibody (M04182). <br>
FKBP5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FKBP5 Antibody (M04182) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04182-fkbp5-primary-antibodies-ihc-testing-4.jpgAnti-FKBP51 Monoclonal Antibody IHC analysis of FKBP5 using anti-FKBP5 antibody (M04182). <br>
FKBP5 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FKBP5 Antibody (M04182) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-desmoglein-2-antibody-m02035-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-wb.jpgAnti-Desmoglein 2 Monoclonal AntibodyWestern blot analysis of Desmoglein 2 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eaat3-antibody-m02367-boster.html2026-03-24T05:19:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02367-wb.jpgAnti-EAAT3 Monoclonal AntibodyWestern blot analysis of EAAT3 expression in Human fetal brain lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurokinin-1-receptor-antibody-m01006-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01006-tacr1-primary-antibodies-wb-testing-1.jpgAnti-Neurokinin 1 Receptor Monoclonal Antibody Western blot analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human MOLT-4 whole cell lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: mouse kidney tissue lysates,<br>
Lane 6: mouse spleen tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurokinin 1 Receptor antigen affinity purified monoclonal antibody (Catalog # M01006) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurokinin 1 Receptor at approximately 48 kDa. The expected band size for Neurokinin 1 Receptor is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01006-tacr1-primary-antibodies-ihc-testing-2.jpgAnti-Neurokinin 1 Receptor Monoclonal Antibody IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006). <br>
Neurokinin 1 Receptor was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01006-tacr1-primary-antibodies-ihc-testing-3.jpgAnti-Neurokinin 1 Receptor Monoclonal Antibody IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006). <br>
Neurokinin 1 Receptor was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01006-tacr1-primary-antibodies-ihc-testing-4.jpgAnti-Neurokinin 1 Receptor Monoclonal Antibody IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006). <br>
Neurokinin 1 Receptor was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd79b-antibody-m01399-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01399-wb.jpgAnti-CD79B Monoclonal AntibodyWestern blot analysis of CD79b expression in Raji cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-muc5b-antibody-m00719-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00719-wb.jpgAnti-MUC5B Monoclonal AntibodyWestern blot analysis of MUC5B expression in Hela cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dmrt1-antibody-m02311-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02311-wb.jpgAnti-DMRT1 Monoclonal AntibodyWestern blot analysis of DMRT1 expression in HL-60 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adar1-antibody-m00869-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00869-adar1-primary-antibodies-wb-testing-1.jpgAnti-ADAR1 Monoclonal AntibodyWestern blot analysis of ADAR1 using anti-ADAR1 antibody (M00869). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Raji whole cell lysates,<br>
Lane 4: human 293T whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1 antigen affinity purified monoclonal antibody (M00869) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADAR1 at approximately 150 kDa. The expected band size for ADAR1 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00869-adar-primary-antibodies-if-testing-2.jpgAnti-ADAR1 Monoclonal Antibody IF analysis of ADAR using anti-ADAR antibody (M00869) and anti-Beta Tubulin antibody (M01857-3).<br>
ADAR was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:100 with rabbit anti-ADAR Antibody (M00869) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00869-adar1-primary-antibodies-ihc-testing-1.jpgAnti-ADAR1 Monoclonal AntibodyIHC analysis of ADAR1 using anti-ADAR1 antibody (M00869). <br>ADAR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ADAR1 Antibody (M00869) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00869-adar1-primary-antibodies-ihc-testing-2.jpgAnti-ADAR1 Monoclonal AntibodyIHC analysis of ADAR1 using anti-ADAR1 antibody (M00869). <br>ADAR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ADAR1 Antibody (M00869) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00869-adar1-primary-antibodies-ihc-testing-3.jpgAnti-ADAR1 Monoclonal AntibodyIHC analysis of ADAR1 using anti-ADAR1 antibody (M00869). <br>ADAR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ADAR1 Antibody (M00869) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm3-antibody-m02580-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02580-wb7.jpgAnti-MCM3 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02580-wb.jpgAnti-MCM3 Monoclonal AntibodyWestern blot analysis of MCM3 expression in (1) K562 cell lysate; (2) RAW264.7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-msmb-antibody-m03041-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03041-wb.jpgAnti-MSMB Monoclonal AntibodyWestern blot analysis of Prostate Secretory Protein/PSP expression in Human prostate cancer lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-k-cadherin-antibody-m06353-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06353-wb.jpgAnti-K-Cadherin-6 CDH6-Monoclonal AntibodyWestern blot analysis of K Cadherin expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actl6a-antibody-m05553-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05553-wb.jpgAnti-ACTL6A Monoclonal AntibodyWestern blot analysis of ACTL6A expression in Ramos cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05553-wb7.jpgAnti-ACTL6A Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05553-wb8.jpgAnti-ACTL6A Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cps1-antibody-m01320-1-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01320-1-cps1-primary-antibodies-wb-testing-1.jpgAnti-CPS1 Monoclonal Antibody Western blot analysis of CPS1 using anti-CPS1 antibody (M01320-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br>
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, <br>
Lane 4: rat liver tissue lysates, <br>
Lane 5: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified monoclonal antibody (M01320-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myo7a-antibody-m03915-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03915-wb.jpgAnti-MYO7A Monoclonal AntibodyWestern blot analysis of Myosin VIIa expression in Y79 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03915-myo7a-primary-antibodies-ihc-testing-2.jpgAnti-MYO7A Monoclonal Antibody IHC analysis of MYO7A using anti-MYO7A antibody (M03915). <br>MYO7A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03915-myo7a-primary-antibodies-ihc-testing-3.jpgAnti-MYO7A Monoclonal Antibody IHC analysis of MYO7A using anti-MYO7A antibody (M03915). <br>MYO7A was detected in a paraffin-embedded section of mouse eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03915-myo7a-primary-antibodies-ihc-testing-4.jpgAnti-MYO7A Monoclonal Antibody IHC analysis of MYO7A using anti-MYO7A antibody (M03915). <br>MYO7A was detected in a paraffin-embedded section of rat eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03915-myo7a-primary-antibodies-ihc-testing-5.jpgAnti-MYO7A Monoclonal Antibody IHC analysis of MYO7A using anti-MYO7A antibody (M03915). <br>MYO7A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fhl1-antibody-m01258-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01258-wb.jpgAnti-FHL1 Monoclonal AntibodyWestern blot analysis of FHL1 expression in Human fetal muscle lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha1-adaptin-antibody-m08806-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08806-wb7.jpgAnti-Alpha1 adaptin AP2A1 Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08806-wb.jpgAnti-Alpha1 adaptin AP2A1 Monoclonal AntibodyWestern blot analysis of Alpha1-adaptin expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup153-antibody-m02183-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02183-wb7.jpgAnti-NUP153 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02183-wb8.jpgAnti-NUP153 Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02183-wb.jpgAnti-NUP153 Monoclonal AntibodyWestern blot analysis of Nup153 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snf2h-antibody-m02687-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02687-wb.jpgAnti-SNF2H SMARCA5 Monoclonal AntibodyWestern blot analysis of SNF2H expression in MCF7 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-coilin-antibody-m02784-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02784-wb.jpgAnti-Coilin Rabbit Monoclonal AntibodyWestern blot analysis of Coilin expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-perp-antibody-m03926-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03926-wb.jpgAnti-PERP Monoclonal AntibodyWestern blot analysis of PERP expression in A431 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tsh-beta-antibody-m04304-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04304-wb.jpgAnti-TSH beta Rabbit Monoclonal AntibodyWestern blot analysis of TSH beta expression in Human pituitary lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-e2f4-antibody-m01920-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01920-wb.jpgAnti-E2F4 Monoclonal AntibodyWestern blot analysis of E2F4 expression in Raji cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gst3-gstp1-antibody-m00394-1-boster.html2026-03-24T05:19:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00394-1-wb.jpgAnti-GST3 / GSTP1 Monoclonal AntibodyWestern blot analysis of GST3 / GST pi expression in PC3 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rrm1-antibody-m01764-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01764-wb.jpgAnti-RRM1 Monoclonal AntibodyWestern blot analysis of RRM1 expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glo1-antibody-m01703-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01703-wb.jpgAnti-GLO1 Monoclonal AntibodyWestern blot analysis of GLO1 expression in Raji cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptbp1-antibody-m01798-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-wb7.jpgAnti-PTBP1 Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-wb.jpgAnti-PTBP1 Monoclonal AntibodyWestern blot analysis of PTBP1 expression in Daudi cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tle1-antibody-m02460-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02460-wb.jpgAnti-TLE1 Rabbit Monoclonal AntibodyWestern blot analysis of TLE 1 expression in SH-SY5Y cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-olfm4-antibody-m04094-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04094-wb.jpgAnti-OLFM4 Monoclonal AntibodyWestern blot analysis of OLFM4 expression in LnCaP cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thyroglobulin-antibody-m00359-3-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-3-wb.jpgAnti-Thyroglobulin TG Monoclonal AntibodyWestern blot analysis of Thyroglobulin expression in Mouse thyroid lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-3-tg-primary-antibodies-ihc-testing-1.jpgAnti-Thyroglobulin TG Monoclonal AntibodyIHC analysis of Thyroglobulin/TG using anti-Thyroglobulin/TG antibody (M00359-3). <br>Thyroglobulin/TG was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Thyroglobulin/TG Antibody (M00359-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pedf-antibody-m02034-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02034-serpinf1-primary-antibodies-wb-testing-1.jpgAnti-PEDF Monoclonal Antibody Western blot analysis of PEDF using anti-PEDF antibody (M02034). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEDF antigen affinity purified monoclonal antibody (Catalog # M02034) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEDF at approximately 50 kDa. The expected band size for PEDF is at 46,50 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd3-antibody-m02571-1-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02571-1-wb.jpgAnti-MBD3 Monoclonal AntibodyWestern blot analysis of MBD3 expression in 293T cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gab1-antibody-m01989-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01989-wb.jpgAnti-GAB1 Monoclonal AntibodyWestern blot analysis of GAB1 expression in HEK293 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nck-antibody-m02260-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02260-nck1-primary-antibodies-wb-testing-1.jpgAnti-Nck NCK1 Monoclonal AntibodyWestern blot analysis of NCK1 using anti-NCK1 antibody (M02260). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human SIHA whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human Jurkat whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat liver tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse liver tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCK1 antigen affinity purified monoclonal antibody (M02260) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCK1 at approximately 43 kDa. The expected band size for NCK1 is at 43 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uroplakin3a-antibody-m06534-1-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06534-1-wb7.jpgAnti-Uroplakin3a UPK3A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06534-1-wb10.jpgAnti-Uroplakin3a UPK3A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06534-1-wb8.jpgAnti-Uroplakin3a UPK3A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06534-1-ihc.jpgAnti-Uroplakin3a UPK3A Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human bladder, using Uroplakin III Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06534-1-wb9.jpgAnti-Uroplakin3a UPK3A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thyroglobulin-antibody-m00359-4-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-4-wb.jpgAnti-Thyroglobulin TG Monoclonal AntibodyWestern blot analysis of Thyroglobulin expression in Human thyroid lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ca125-muc16-antibody-m01641-1-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01641-1-muc16-primary-antibodies-ihc-testing-1.jpgAnti-CA125 / MUC16 Monoclonal AntibodyIHC analysis of CA125/MUC16 using anti-CA125/MUC16 antibody (M01641-1). <br>
ITGAE was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CA125/MUC16 Antibody (M01641-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcl1-antibody-m03713-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03713-wb.jpgAnti-TCL1 Monoclonal AntibodyWestern blot analysis of TCL1 expression in Ramos cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prostatic-acid-phosphatase-antibody-m02082-1-boster.html2026-03-24T05:19:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02082-1-wb.jpgAnti-Prostatic Acid Phosphatase ACPP Monoclonal AntibodyWestern blot analysis of PAP ( prostatic acid phosphatase) expression in human prostate cancer lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-li-cadherin-antibody-m06389-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06389-wb7.jpgAnti-LI-Cadherin-17 CDH17-Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06389-wb.jpgAnti-LI-Cadherin-17 CDH17-Rabbit Monoclonal AntibodyWestern blot analysis of LI-Cadherin expression in HT-29 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxp1-antibody-m00723-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00723-1-wb.jpgAnti-FOXP1 Monoclonal AntibodyWestern blot analysis of FOXP1 expression in Daudi cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lin28-antibody-m01966-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01966-1-wb.jpgAnti-LIN28 LIN28A Monoclonal AntibodyWestern blot analysis of LIN28 expression in NCCIT cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cadherin-16-antibody-m07518-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07518-1-wb.jpgAnti-Cadherin 16 CDH16 Monoclonal AntibodyWestern blot analysis of Cadherin 16 expression in Human fetal kidney lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd53-antibody-m06249-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06249-wb.jpgAnti-CD53 Monoclonal AntibodyWestern blot analysis of CD53 expression in Jurkat cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myogenin-antibody-m03238-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03238-1-wb.jpgAnti-Myogenin Monoclonal AntibodyWestern blot analysis of Myogenin expression in C2C12 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tpo-antibody-m00320-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00320-wb.jpgAnti-TPO Monoclonal AntibodyWestern blot analysis of TPO expression in Human thyroid lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00320-tpo-primary-antibodies-ihc-testing-1.jpgAnti-TPO Monoclonal AntibodyIHC analysis of TPO using anti-TPO antibody (M00320). <br>TPO was detected in a paraffin-embedded section of human thyroid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPO Antibody (M00320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00320-tpo-primary-antibodies-ihc-testing-2.jpgAnti-TPO Monoclonal AntibodyIHC analysis of TPO using anti-TPO antibody (M00320). <br>TPO was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPO Antibody (M00320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-villin-1-antibody-m04205-2-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04205-2-wb.jpgAnti-Villin-1 VIL1 Monoclonal AntibodyWestern blot analysis of Villin-1 expression in CaCo-2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-drb1-antibody-m00568-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00568-1-wb.jpgAnti-HLA-Drb1 Monoclonal AntibodyWestern blot analysis of HLA-Drb1 expression in Ramos cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd10-antibody-m04065-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04065-1-wb.jpgAnti-CD10 MME Monoclonal AntibodyWestern blot analysis of CD10 expression in Ramos cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd25-antibody-m00214-1-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00214-1-ihc.jpgAnti-CD25 IL2RA Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human tonsil, using CD25 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scgn-antibody-m10629-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10629-wb7.jpgAnti-SCGN Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10629-wb.jpgAnti-SCGN Monoclonal AntibodyWestern blot analysis of SCGN expression in human fetal brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10629-wb8.jpgAnti-SCGN Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10629-wb9.jpgAnti-SCGN Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-placental-lactogen-antibody-m05177-boster.html2026-03-24T05:19:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05177-wb.jpgAnti-Placental lactogen CSH1 Monoclonal AntibodyWestern blot analysis of Placental lactogen expression in Human placenta lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spta1-antibody-m03376-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03376-wb.jpgAnti-SPTA1 Rabbit Monoclonal AntibodyWestern blot analysis of SPTA1 expression in K562 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-podxl-antibody-m03359-1-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00328-nox2-primary-antibodies-wb-testing-1.jpgAnti-PODXL Monoclonal AntibodyWestern blot analysis of PODXL using anti-PODXL antibody (M03359-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PODXL antigen affinity purified monoclonal antibody (M03359-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PODXL at approximately 165 kDa. The expected band size for PODXL is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03359-1-icc1.jpgAnti-PODXL Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iba1-antibody-m01394-1-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-1-iba1-primary-antibodies-wb-testing-1.jpgAnti-IBA1 AIF1 Monoclonal Antibody Western blot analysis of IBA1 using anti-IBA1 antibody (M01394-1). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human U937 whole cell lysates,<br>
Lane 3: human HL-60 whole cell lysates,<br>
Lane 4: human HEL whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBA1 antigen affinity purified monoclonal antibody (M01394-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IBA1 at approximately 17 kDa. The expected band size for IBA1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-1-iba1-primary-antibodies-ihc-testing-2.jpgAnti-IBA1 AIF1 Monoclonal Antibody IHC analysis of IBA1 using anti-IBA1 antibody (M01394-1). <br>IBA1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IBA1 Antibody (M01394-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fsh-beta-fshb-antibody-m01885-1-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01885-1-wb.jpgAnti-FSH beta (FSHB) Monoclonal AntibodyWestern blot analysis of FSH beta (FSHB) expression in Human pituitary lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbx21-antibody-m00404-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00404-ihc.jpgAnti-TBX21 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human spleen, using TBX21 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tdt-antibody-m05546-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05546-dntt-primary-antibodies-wb-testing-1.jpgAnti-TDT DNTT Monoclonal Antibody Western blot analysis of TDT using anti-TDT antibody (M05546). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human MOLT-4 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDT antigen affinity purified monoclonal antibody (M05546) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDT at approximately 59 kDa. The expected band size for TDT is at 59 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c4a-antibody-m01095-1-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01095-1-wb.jpgAnti-C4A Monoclonal AntibodyWestern blot analysis of C4A expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01095-1-wb7.jpgAnti-C4A Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tfe3-antibody-m01791-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01791-wb7.jpgAnti-TFE3 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01791-ihc.jpgAnti-TFE3 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human bladder, using TFE3 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cox2-antibody-m00084-1-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00084-1-wb7.jpgAnti-Cox2 PTGS2 Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00084-1-wb8.jpgAnti-Cox2 PTGS2 Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00084-1-wb.jpgAnti-Cox2 PTGS2 Monoclonal AntibodyWestern blot analysis of Cox2 expression in A549 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abhd5-antibody-m03209-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03209-wb.jpgAnti-ABHD5 Monoclonal AntibodyWestern blot analysis of ABHD5 expression in HepG2 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcet2-antibody-m12995-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12995-ihc.jpgAnti-GCET2 Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human bladder cancer, using GCET2 Antibody.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pbrm1-antibody-m01130-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01130-wb.jpgAnti-PBRM1 Rabbit Monoclonal AntibodyWestern blot analysis of PBRM1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01130-pbrm1-primary-antibodies-ihc-testing-1.jpgAnti-PBRM1 Rabbit Monoclonal AntibodyIHC analysis of PBRM1 using anti-PBRM1 antibody (M01130). <br>PBRM1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01130-pbrm1-primary-antibodies-ihc-testing-2.jpgAnti-PBRM1 Rabbit Monoclonal AntibodyIHC analysis of PBRM1 using anti-PBRM1 antibody (M01130). <br>PBRM1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01130-pbrm1-primary-antibodies-ihc-testing-3.jpgAnti-PBRM1 Rabbit Monoclonal AntibodyIHC analysis of PBRM1 using anti-PBRM1 antibody (M01130). <br>PBRM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01130-pbrm1-primary-antibodies-ihc-testing-4.jpgAnti-PBRM1 Rabbit Monoclonal AntibodyIHC analysis of PBRM1 using anti-PBRM1 antibody (M01130). <br>PBRM1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PBRM1 Antibody (M01130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fdft1-antibody-m05118-boster.html2026-03-24T05:19:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05118-wb7.jpgAnti-FDFT1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05118-wb10.jpgAnti-FDFT1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05118-wb8.jpgAnti-FDFT1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05118-wb.jpgAnti-FDFT1 Monoclonal AntibodyWestern blot analysis of FDFT1 expression in (1) HepG2 cell lysate; (2) RAW264.7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05118-wb9.jpgAnti-FDFT1 Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif4e-antibody-m00135-boster.html2026-03-24T05:19:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00135-eif4e-primary-antibodies-wb-testing-1.jpgAnti-EIF4E Monoclonal Antibody Western blot analysis of EIF4E using anti-EIF4E antibody (M00135). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse HEPA1-2 whole cell lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crx-antibody-m02202-boster.html2026-03-24T05:19:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02202-wb.jpgAnti-CRX Monoclonal AntibodyWestern blot analysis of CRX expression in Y79 cell lysate.
https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-islet1-antibody-m02969-1-boster.html2026-03-24T05:19:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02969-1-wb.jpgAnti-Islet1 Monoclonal AntibodyWestern blot analysis of Islet1 expression in HeLa cell lysate.
https://www.bosterbio.com/products/primary-antibodies/anti-usp16-picoband-trade-antibody-a05795-boster.html2026-03-24T05:19:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05795-USP16-IHC-test-1.jpgAnti-USP16 Antibody Picoband®Sample Type :Human brain stem cells (NT2) Primary Antibody Dilution : 1:500Secondary Antibody :Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :Red: USP16 Blue: DAPIGene Name :USP16Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Sciencehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05795-USP16-WB-test-2.jpgAnti-USP16 Antibody Picoband®WB Suggested Anti-USP16 Antibody Titration: 2.5ug/mlPositive Control: HepG2 cell lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-usp16-primary-antibodies-wb-testing-1.jpgAnti-USP16 Antibody Picoband® Western blot analysis of USP16 using anti-USP16 antibody (A05795). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human U20S whole cell lysates, <br>
Lane 3: human T-47D whole cell lysates, <br>
Lane 4: human CACO-2 whole cell lysates, <br>
Lane 5: monkey heart tissue lysates, <br>
Lane 6: monkey kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP16 antigen affinity purified polyclonal antibody (Catalog # A05795) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP16 at approximately 100KD. The expected band size for USP16 is at 100KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-usp16-primary-antibodies-ihc-testing-2.jpgAnti-USP16 Antibody Picoband® IHC analysis of USP16 using anti-USP16 antibody (A05795). <br>
USP16 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-USP16 Antibody (A05795) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-usp16-primary-antibodies-ihc-testing-3.jpgAnti-USP16 Antibody Picoband® IHC analysis of USP16 using anti-USP16 antibody (A05795). <br>
USP16 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-USP16 Antibody (A05795) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-usp16-primary-antibodies-if-testing-4.jpgAnti-USP16 Antibody Picoband® IF analysis of USP16 using anti-USP16 antibody (A05795). <br>
USP16 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-USP16 Antibody (A05795) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-usp16-primary-antibodies-fcm-testing-5.pngAnti-USP16 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-USP16 antibody (A05795).<br>Overlay histogram showing A431 cells stained with A05795 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP16 Antibody (A05795,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspa2-picoband-trade-antibody-m03474-1-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03474-1-HSPA2-primary-antibodies-IHC-testing-1.jpgAnti-HSPA2 Antibody Picoband® (monoclonal, 4A4) IHC analysis of HSPA2 using anti-HSPA2 antibody (M03474-1). <br>HSPA2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HSPA2 Antibody (M03474-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03474-1-HSPA2-primary-antibodies-WB-testing-2.jpgAnti-HSPA2 Antibody Picoband® (monoclonal, 4A4) Western blot analysis of HSPA2 using anti-HSPA2 antibody (M03474-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate, <br>Lane 2: human MDA-MB-231 whole cell lysate,<br>Lane 3: human COLO-320 whole cell lysate,<br>Lane 4: human PANC-1 whole cell lysate.<br>Lane 5: human HT1080 whole cell lysate,<br>Lane 6: human MDA-MB-453 whole cell lysate,<br>Lane 7: human HepG2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPA2 antigen affinity purified monoclonal antibody (Catalog # M03474-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03474-1-hspa2-primary-antibodies-if-testing-3.jpgAnti-HSPA2 Antibody Picoband® (monoclonal, 4A4) IF analysis of HSPA2 using anti-HSPA2 antibody (M03474-1). <br> HSPA2 was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA2 Antibody (M03474-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03474-1-hspa2-primary-antibodies-fc-testing-4.pngAnti-HSPA2 Antibody Picoband® (monoclonal, 4A4) Flow Cytometry analysis of PC-3 cells using anti-HSPA2 antibody (M03474-1). <br> Overlay histogram showing PC-3 cells stained with M03474-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA2 Antibody (M03474-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03474-1-hspa2-primary-antibodies-wb-testing-5.jpgAnti-HSPA2 Antibody Picoband® (monoclonal, 4A4) Western blot analysis of HSPA2 using anti-HSPA2 antibody (M03474-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat kidney tissue lysates, <br> Lane 4: rat testicular tissue lysates, <br> Lane 5: mouse lung tissue lysates, <br> Lane 6: mouse liver tissue lysates, <br> Lane 7: mouse kidney tissue lysates, <br> Lane 8: mouse testicular tissue lysates, <br> Lane 9: mouse RAW246.7 whole cell lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPA2 antigen affinity purified monoclonal antibody (Catalog # M03474-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp70-picoband-trade-antibody-m00949-2-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00949-2-Hsp70-primary-antibodies-IHC-testing-1.jpgAnti-Hsp70 HSPA1A Antibody Picoband® (monoclonal, 3H5) IHC analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). <br>Hsp70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Hsp70 Antibody (M00949-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00949-2-Hsp70-primary-antibodies-WB-testing-2.jpgAnti-Hsp70 HSPA1A Antibody Picoband® (monoclonal, 3H5) Western blot analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate,<br>Lane 2: human COLO-320 whole cell lysate,<br>Lane 3: human SW620 whole cell lysate,<br>Lane 4: human A431 whole cell lysate,<br>Lane 5: human A549 whole cell lysate,<br>Lane 6: human HepG2 whole cell lysate,<br>Lane 7: human PANC-1 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hsp70 antigen affinity purified monoclonal antibody (Catalog # M00949-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00949-2-hsp70-primary-antibodies-fcm-testing-3.jpgAnti-Hsp70 HSPA1A Antibody Picoband® (monoclonal, 3H5) Flow Cytometry analysis of U20S cells using anti-Hsp70 antibody (M00949-2). <br> Overlay histogram showing U20S cells stained with M00949-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp70 Antibody (M00949-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00949-2-hsp70-primary-antibodies-if-testing-4.jpgAnti-Hsp70 HSPA1A Antibody Picoband® (monoclonal, 3H5) IF analysis of Hsp70 using anti-Hsp70 antibody (M00949-2). <br>
Hsp70 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Hsp70 Antibody (M00949-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smc3-picoband-trade-antibody-m01930-1-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01930-1-smc3-primary-antibodies-wb-testing-1.jpgAnti-SMC3 Antibody Picoband® (monoclonal, 4C12) Western blot analysis of SMC3 using anti-SMC3 antibody (M01930-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human MDA-MB-453 whole cell lysates, <br>
Lane 5: rat thymus tissue lysates, <br>
Lane 6: rat liver tissue lysates, <br>
Lane 7: mouse thymus tissue lysates, <br>
Lane 8: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMC3 antigen affinity purified monoclonal antibody (Catalog # M01930-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SMC3 at approximately 142 kDa. The expected band size for SMC3 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01930-1-SMC3-primary-antibodies-IHC-testing-2.jpgAnti-SMC3 Antibody Picoband® (monoclonal, 4C12) IHC analysis of SMC3 using anti-SMC3 antibody (M01930-1). <br>SMC3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-SMC3 Antibody (M01930-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01930-1-smc3-primary-antibodies-ihc-testing-3.jpgAnti-SMC3 Antibody Picoband® (monoclonal, 4C12) IHC analysis of SMC3 using anti-SMC3 antibody (M01930-1). <br>SMC3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-SMC3 Antibody (M01930-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ampk-beta-2-picoband-trade-antibody-m05077-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05077-AMPK-beta-2-primary-antibodies-FC-testing-1.jpgAnti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).<br>Overlay histogram showing PC-3 cells stained with M05077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05077-AMPK-beta-2-primary-antibodies-WB-testing-2.jpgAnti-AMPK beta 2 PRKAB2 Antibody Picoband® (monoclonal, 6G1) Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (M05077). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate, <br>Lane 2: human placenta tissue lysate,<br>Lane 3: human 293T whole cell lysate,<br>Lane 4: human A549 whole cell lysate,<br>Lane 5: human A375 whole cell lysate,<br>Lane 6: human A431 whole cell lysate,<br>Lane 7: human U20S whole cell lysate,<br>Lane 8: human K562 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AMPK beta 2 antigen affinity purified monoclonal antibody (Catalog # M05077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rbap48-picoband-trade-antibody-m02702-1-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02702-1-RbAp48-primary-antibodies-IHC-testing-1.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br>RbAp48 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-wb-testing-10.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) Western blot analysis of RbAp48 using anti-RbAp48 antibody (M02702-1).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates<br> Lane 2: rat thymus tissue lysates<br> Lane 3: mouse spleen tissue lysates <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RbAp48 antigen affinity purified monoclonal antibody (Catalog # M02702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 55KD. The expected band size for RbAp48 is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02702-1-RbAp48-primary-antibodies-IHC-testing-2.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br>RbAp48 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02702-1-RbAp48-primary-antibodies-WB-testing-3.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) Western blot analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysate,<br>Lane 2: human Jurkat whole cell lysate,<br>Lane 3: human Hela whole cell lysate,<br>Lane 4: human PANC-1 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RbAp48 antigen affinity purified monoclonal antibody (Catalog # M02702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-ihc-testing-4.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br> RbAp48 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-ihc-testing-5.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br> RbAp48 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-ihc-testing-6.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br> RbAp48 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-ihc-testing-7.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br> RbAp48 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-ihc-testing-8.jpgAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1). <br> RbAp48 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02702-1-rbap48-primary-antibodies-fc-testing-9.pngAnti-RbAp48 RBBP4 Antibody Picoband® (monoclonal, 9F3) Flow Cytometry analysis of SiHa cells using anti-RbAp48 antibody (M02702-1).<br> Overlay histogram showing SiHa cells stained with M02702-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RbAp48 Antibody (M02702-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytochrome-c-picoband-trade-antibody-m03529-5-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03529-5-Cytochrome-C-primary-antibodies-IHC-testing-4.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) IHC analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>Cytochrome C was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Cytochrome C Antibody (M03529-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03529-5-Cytochrome-C-primary-antibodies-IHC-testing-5.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) IHC analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Cytochrome C Antibody (M03529-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-5-cytochrome-c-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate, <br>Lane 2: human HepG2 whole cell lysate<br>Lane 3: human K562 whole cell lysate,<br>Lane 4: human Caco-2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome C antigen affinity purified monoclonal antibody (Catalog # M03529-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-5-cytochrome-c-primary-antibodies-fc-testing-7.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (M03529-5). <br>Overlay histogram showing A431 cells stained with M03529-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (M03529-5,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-5-cytochrome-c-primary-antibodies-wb-testing-2.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysate,<br>Lane 2: rat heart tissue lysate,<br>Lane 3: rat kidney tissue lysate,<br>Lane 4: rat testis tissue lysate,<br>Lane 5: mouse brain tissue lysate,<br>Lane 6: mouse heart tissue lysate,<br>Lane 7: mouse kidney tissue lysate,<br>Lane 8: mouse testis tissue lysate,<br>Lane 9: mouse Neuro-2a whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody (Catalog # M03529-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-5-cytochrome-c-primary-antibodies-ihc-testing-3.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) IHC analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>Cytochrome C was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Cytochrome C Antibody (M03529-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-5-cytochrome-c-primary-antibodies-if-testing-6.jpgAnti-Cytochrome C CYCS Antibody Picoband® (monoclonal, 15F10) IF analysis of Cytochrome C using anti-Cytochrome C antibody (M03529-5). <br>
Cytochrome C was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Cytochrome C Antibody (M03529-5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gstm1-picoband-trade-antibody-m00569-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00569-GSTM1-primary-antibodies-FC-testing-1.jpgAnti-GSTM1 Antibody Picoband® (monoclonal, 11F2) Flow Cytometry analysis of U20S cells using anti-GSTM1 antibody (M00569). <br>Overlay histogram showing U20S cells stained with M00569 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (M00569,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00569-GSTM1-primary-antibodies-FC-testing-2.jpgAnti-GSTM1 Antibody Picoband® (monoclonal, 11F2) Flow Cytometry analysis of HELA cells using anti-GSTM1 antibody (M00569). <br>Overlay histogram showing HELA cells stained with M00569 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (M00569,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00569-GSTM1-primary-antibodies-IHC-testing-3.jpgAnti-GSTM1 Antibody Picoband® (monoclonal, 11F2) IHC analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br>GSTM1 was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-GSTM1 Antibody (M00569) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00569-GSTM1-primary-antibodies-IHC-testing-4.jpgAnti-GSTM1 Antibody Picoband® (monoclonal, 11F2) IHC analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br>GSTM1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-GSTM1 Antibody (M00569) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00569-gstm1-primary-antibodies-wb-testing-5_1.jpgAnti-GSTM1 Antibody Picoband® (monoclonal, 11F2) Western blot analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela, whole cell lysate,<br> Lane 2: human T-47D whole cell lysate,<br> Lane 3: rat brain tissue lysate,<br> Lane 4: rat lung tissue lysate,<br> Lane 5: rat stomach tissue lysate, <br> Lane 6: mouse lung tissue lysate,<br> Lane 7: mouse stomach tissue lysate,<br> Lane 8: mouse kidney tissue lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GSTM1 antigen affinity purified monoclonal antibody (Catalog # M00569) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arsa-picoband-trade-antibody-m02583-boster.html2026-03-25T05:22:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02583-ARSA-primary-antibodies-WB-testing-2.jpgAnti-ARSA Antibody Picoband® (monoclonal, 4C10) Western blot analysis of ARSA using anti-ARSA antibody (M02583). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysate,<br>Lane 2: rat liver tissue lysate,<br>Lane 3: rat brain tissue lysate,<br>Lane 4: rat lung tissue lysate,<br>Lane 5: mouse testis tissue lysate,<br>Lane 6: mouse liver tissue lysate,<br>Lane 7: mouse brain tissue lysate,<br>Lane 8: mouse lung tissue lysate,<br>Lane 9: mouse HEPA1-6 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody (Catalog # M02583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02583-ARSA-primary-antibodies-WB-testing-3.jpgAnti-ARSA Antibody Picoband® (monoclonal, 4C10) Western blot analysis of ARSA using anti-ARSA antibody (M02583). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A375 whole cell lysate,<br>Lane 2: human A549 whole cell lysate,<br>Lane 3: human SMMC-7721 whole cell lysate.<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody (Catalog # M02583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02583-ARSA-primary-antibodies-FC-testing-1.jpgAnti-ARSA Antibody Picoband® (monoclonal, 4C10) Flow Cytometry analysis of ANA-1 cells using anti-ARSA antibody (M02583). <br>Overlay histogram showing ANA-1 cells stained with M02583 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ARSA Antibody (M02583,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02583-arsa-primary-antibodies-fc-testing-4.pngAnti-ARSA Antibody Picoband® (monoclonal, 4C10) Flow Cytometry analysis of Raji cells using anti-ARSA antibody (M02583). <br> Overlay histogram showing Raji cells stained with M02583 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (M02583, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02583-arsa-primary-antibodies-ihc-testing-5.jpgAnti-ARSA Antibody Picoband® (monoclonal, 4C10) IHC analysis of ARSA using anti-ARSA antibody (M02583). <br> ARSA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARSA Antibody (M02583) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-casr-picoband-trade-antibody-m00574-boster.html2026-03-24T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00574-casr-primary-antibodies-wb-testing-1.jpgAnti-CASR Antibody Picoband® (monoclonal, 11E9) Western blot analysis of CASR using anti-CASR antibody (M00574). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate,<br>Lane 2: human A549 whole cell lysate,<br>Lane 3: human 22RV1 whole cell lysate,<br>Lane 4: human HepG2 whole cell lysate,<br>Lane 5: human Caco-2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CASR antigen affinity purified monoclonal antibody (Catalog # M00574) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
https://www.bosterbio.com/picokine-elisa-kits/human-insulin-elisa-kit-ek7000-boster.html2026-03-24T05:20:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7000_1_1.pngHuman Insulin OneStep ELISA KitHuman Insulin ELISA Kit standard curve
https://www.bosterbio.com/products/all-recombinant-proteins/human-tnf-alpha-recombinant-protein-r00002-8-boster.html2026-03-24T05:20:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman TNF alpha Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-tnf-alpha-recombinant-protein-r00002-9-boster.html2026-03-24T05:20:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse TNF alpha Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-10-recombinant-protein-r00021-3-boster.html2026-03-24T05:20:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-10 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-vegf-a-recombinant-protein-r00045-4-boster.html2026-03-24T05:20:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse VEGF-A Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-vegf-a-recombinant-protein-r00045-7-boster.html2026-03-24T05:20:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat VEGF-A Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-cxcl12-recombinant-protein-r00053-boster.html2026-03-24T05:20:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse CXCL12 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-13-recombinant-protein-r00077-4-boster.html2026-03-24T05:20:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-13 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-6-recombinant-protein-r00102-5-boster.html2026-03-24T05:20:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-6 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-6-recombinant-protein-r00102-6-boster.html2026-03-24T05:20:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-6 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-fgf-2-recombinant-protein-r00121-2-boster.html2026-03-24T05:20:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse FGF-2 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-18-recombinant-protein-r00124-2-boster.html2026-03-24T05:20:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-18 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-4-recombinant-protein-r00230-5-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-4 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-4-recombinant-protein-r00230-6-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-4 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-cxcl10-recombinant-protein-r00278-4-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman CXCL10 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-cxcl10-recombinant-protein-r00278-5-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse CXCL10 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-tnfsf11-recombinant-protein-r00363-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse TNFSF11 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-tnfsf11-recombinant-protein-r00363-1-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat TNFSF11 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-2-recombinant-protein-r00387-5-boster.html2026-03-24T05:20:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-2 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-2-recombinant-protein-r00387-6-boster.html2026-03-24T05:20:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-2 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-ifn-gamma-recombinant-protein-r00393-7-boster.html2026-03-24T05:20:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IFN gamma Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-ifn-gamma-recombinant-protein-r00393-8-boster.html2026-03-24T05:20:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IFN gamma Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-ccl3-recombinant-protein-r00405-2-boster.html2026-03-24T05:20:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse CCL3 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-17a-recombinant-protein-r00421-3-boster.html2026-03-24T05:20:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-17A Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-17a-recombinant-protein-r00421-4-boster.html2026-03-24T05:20:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-17A Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-il-17a-recombinant-protein-r00421-7-boster.html2026-03-24T05:20:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat IL-17A Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-8-recombinant-protein-r00423-8-boster.html2025-12-29T08:09:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-8 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-m-csf-recombinant-protein-r00620-boster.html2026-03-24T05:20:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse M-CSF Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-ccl4-recombinant-protein-r00703-3-boster.html2026-03-24T05:20:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman CCL4 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-ccl4-recombinant-protein-r00703-4-boster.html2026-03-24T05:20:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse CCL4 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-ccl4-recombinant-protein-r00703-6-boster.html2026-03-24T05:20:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat CCL4 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-gm-csf-recombinant-protein-r00911-4-boster.html2026-03-24T05:20:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman GM-CSF Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-gm-csf-recombinant-protein-r00911-5-boster.html2026-03-24T05:20:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse GM-CSF Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-baff-recombinant-protein-r01257-3-boster.html2026-03-24T05:20:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse BAFF Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-b7-h2-recombinant-protein-r01965-boster.html2026-03-24T05:20:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse B7-H2 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-ifn-beta-recombinant-protein-r02041-2-boster.html2026-03-24T05:20:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IFN beta Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-april-recombinant-protein-r02417-1-boster.html2026-03-24T05:20:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman APRIL Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-april-recombinant-protein-r02417-2-boster.html2026-03-24T05:20:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse APRIL Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-april-recombinant-protein-r02417-4-boster.html2026-03-24T05:20:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat APRIL Recombinant ProteinBoster Kit Box
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https://www.bosterbio.com/products/all-recombinant-proteins/human-vegf-d-recombinant-protein-r04809-boster.html2026-03-24T05:20:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman VEGF-D Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-34-recombinant-protein-r06903-boster.html2026-03-24T05:20:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-34 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-il-1-beta-recombinant-protein-r00101-6-boster.html2026-03-24T05:20:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IL-1 beta Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-1-beta-recombinant-protein-r00101-7-boster.html2026-03-24T05:20:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-1 beta Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-il-1-alpha-recombinant-protein-r01144-5-boster.html2026-03-24T05:20:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse IL-1 alpha Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/human-cxcl9-recombinant-protein-r01397-3-boster.html2026-03-24T05:20:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman CXCL9 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/mouse-cxcl9-recombinant-protein-r01397-4-boster.html2026-03-24T05:20:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse CXCL9 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/products/all-recombinant-proteins/rat-cxcl9-recombinant-protein-r01397-5-boster.html2026-03-24T05:20:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRat CXCL9 Recombinant ProteinBoster Kit Box
https://www.bosterbio.com/picokine-elisa-kits/human-progesterone-elisa-kit-ek7004-boster.html2026-03-24T05:20:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7004_1.pngHuman Progesterone(P) ELISA Kit (Competitive ELISA)Human Progesterone(P) ELISA Kit standard curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Progesterone(P) ELISA Kit (Competitive ELISA)
https://www.bosterbio.com/picokine-elisa-kits/human-testosterone-elisa-kit-ek7005-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7005_1.pngHuman Testosterone(T) ELISA Kit (Competitive ELISA)Human Testosterone(T) ELISA Kit standard curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Testosterone(T) ELISA Kit (Competitive ELISA)
https://www.bosterbio.com/picokine-elisa-kits/human-prolactin-elisa-kit-ek7006-boster.html2026-03-24T05:20:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Prolactin(PRL) OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7006_1.pngHuman Prolactin(PRL) OneStep ELISA KitHuman Prolactin(PRL) ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-triiodothyronine-elisa-kit-ek7010-boster.html2026-03-24T05:20:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Triiodothyronine (T3) ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7010.pngHuman Triiodothyronine (T3) ELISA KitHuman T3 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-thyroxine-elisa-kit-ek7012-boster.html2026-03-24T05:20:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7012_1.pngHuman Thyroxine (T4) ELISA KitHuman T4 ELISA Kit standard curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Thyroxine (T4) ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/human-alpha-fetoprotein-onestep-elisa-kit-afp-ek7020-boster.html2026-03-24T05:20:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7020_2.pngHuman alpha Fetoprotein OneStep ELISA Kit (AFP)Human AFP ELISA Kit Standard Curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman alpha Fetoprotein OneStep ELISA Kit (AFP)
https://www.bosterbio.com/picokine-elisa-kits/human-ca19-9-onestep-elisa-kit-ek7028-boster.html2026-03-24T05:20:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman CA19-9 OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7028.pngHuman CA19-9 OneStep ELISA KitHuman CA19-9 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-ca-125-onestep-elisa-kit-ek7029-boster.html2026-03-24T05:20:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman CA-125 OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7029.jpgHuman CA-125 OneStep ELISA KitHuman CA125 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-ca15-3-elisa-kit-ek7030-boster.html2026-03-24T05:20:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman CA15-3 ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7030.pngHuman CA15-3 ELISA KitHuman CA15-3 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-carcinoembryonic-antigen-cea-onestep-elisa-kit-ek7031-boster.html2026-03-24T05:20:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Carcinoembryonic Antigen (CEA) OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7031.pngHuman Carcinoembryonic Antigen (CEA) OneStep ELISA KitHuman CEA ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-c-peptide-onestep-elisa-kit-ek7039-boster.html2026-03-24T05:20:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman C-Peptide OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7039_1.pngHuman C-Peptide OneStep ELISA KitHuman C-peptide(C-P) ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-estradiol-elisa-kit-ek7049-boster.html2026-03-24T05:20:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7049_1.pngHuman Estrodial (E2) ELISA Kit (Competitive ELISA)Human Estrodial (E2) ELISA Kit standard curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Estrodial (E2) ELISA Kit (Competitive ELISA)
https://www.bosterbio.com/picokine-elisa-kits/human-free-triiodothyronine-ft3-elisa-kit-ek7053-boster.html2026-03-24T05:20:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Free Triiodothyronine (FT3) ELISA Kit (Competitive ELISA)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7053.pngHuman Free Triiodothyronine (FT3) ELISA Kit (Competitive ELISA)Human FT3 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-free-thyroxine-ft4-elisa-kit-ek7054-boster.html2026-03-24T05:20:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Free Thyroxine (FT4) ELISA Kit (Competitive ELISA)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7054_1.pngHuman Free Thyroxine (FT4) ELISA Kit (Competitive ELISA)Human FT4 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-ferritin-onestep-elisa-kit-ek7055-boster.html2026-03-24T05:20:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Ferritin OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7055.pngHuman Ferritin OneStep ELISA KitHuman Ferritin(Ferr) ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-follicle-stimulating-hormone-fsh-onestep-elisa-kit-ek7056-boster.html2026-03-24T05:20:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/l/elisa_kit_package.pngHuman Follicle Stimulating Hormone (FSH) OneStep ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7056_1.pngHuman Follicle Stimulating Hormone (FSH) OneStep ELISA KitHuman FSH ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/beta-human-chorionic-gonadotropin-hcg-onestep-elisa-kit-ek7062-boster.html2026-03-24T05:20:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7062_1.pngBeta-human Chorionic Gonadotropin(β-hCG) ELISA Kitβ-hCG ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-luteinizing-hormone-lh-onestep-elisa-kit-ek7067-boster.html2026-03-24T05:20:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7067_1.pngHuman Luteinizing Hormone (LH) OneStep ELISA KitHuman Luteinizing Hormone(LH) ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-prostate-specific-antigen-psa-onestep-elisa-kit-ek7080-boster.html2026-03-24T05:20:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7080.pngHuman Prostate Specific Antigen (PSA) OneStep ELISA KitHuman PSA ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-thyroid-stimulating-hormone-tsh-onestep-elisa-kit-ek7098-boster.html2026-03-24T05:20:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7098.pngHuman Thyroid Stimulating Hormone (TSH) OneStep ELISA KitHuman TSH ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/anti-interferon-gamma-picoband-trade-antibody-a00393-1-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-1-interferon-gamma-primary-antibodies-elisa-testing-1.jpgAnti-Interferon gamma IFNG Antibody Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.<br>
Use in combination with reagents from Rat Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0374).
https://www.bosterbio.com/products/primary-antibodies/anti-interferon-gamma-picoband-trade-antibody-a00393-2-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-2-interferon-gamma-primary-antibodies-elisa-testing-1.jpgAnti-Interferon gamma IFNG Antibody Sandwich ELISA - Recombinant mouse Interferon gamma protein standard curve.<br>
Use in combination with reagents from Mouse Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0375).
https://www.bosterbio.com/products/primary-antibodies/anti-aes-picoband-trade-antibody-a01757-1-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01757-1-AES-primary-antibodies-WB-testing-1.jpgAnti-AES Antibody Picoband® Western blot analysis of AES using anti-AES antibody (A01757-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human U-87MG whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AES antigen affinity purified polyclonal antibody (Catalog # A01757-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AES at approximately 22KD. The expected band size for AES is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01757-1-aes-primary-antibodies-ihc-testing-2.jpgAnti-AES Antibody Picoband® IHC analysis of AES using anti-AES antibody (A01757-1). <br> AES was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AES Antibody (A01757-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01757-1-aes-primary-antibodies-if-testing-3.jpgAnti-AES Antibody Picoband® IF analysis of AES using anti-AES antibody (A01757-1). <br>
AES was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AES Antibody (A01757-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01757-1-aes-primary-antibodies-fcm-testing-4_1.jpgAnti-AES Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-AES antibody (A01757-1).<br>
Overlay histogram showing U87 cells stained with A01757-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AES Antibody (A01757-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ahr-picoband-trade-antibody-a00225-2-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-2-ahr-primary-antibodies-wb-testing-1.jpgAnti-AHR Antibody Picoband® Western blot analysis of AHR using anti-AHR antibody (A00225-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # A00225-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 100 kDa. The expected band size for AHR is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-2.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-3.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-4.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-5.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-6.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-7.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-IHC-testing-8.jpgAnti-AHR Antibody Picoband® IHC analysis of AHR using anti-AHR antibody (A00225-2).<br>AHR was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (A00225-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00225-2-AHR-primary-antibodies-FC-testing-9.jpgAnti-AHR Antibody Picoband®9. Flow Cytometry analysis of U87 cells using anti-AHR antibody (A00225-2).<br>Overlay histogram showing U87 cells stained with A00225-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody (A00225-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-2-ahr-primary-antibodies-fc-testing-10.jpgAnti-AHR Antibody Picoband®10. Flow Cytometry analysis of U937 cells using anti-AHR antibody (A00225-2). <br> Overlay histogram showing U937 cells stained with A00225-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody (A00225-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-alpha-1-microglobulin-picoband-trade-antibody-a02419-3-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02419-3-Alpha-1-microglobulin-primary-antibodies-WB-testing-1.jpgAnti-Alpha 1 microglobulin/AMBP Antibody Picoband® Western blot analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human HEP-2 whole cell lysate,<br>Lane 3: human Hela whole cell lysate,<br>Lane 4: human A549 whole cell lysate,<br>Lane 5: human PANC-1 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 1 microglobulin antigen affinity purified polyclonal antibody (Catalog # A02419-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 1 microglobulin at approximately 45KD. The expected band size for Alpha 1 microglobulin is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02419-3-Alpha-1-microglobulin-primary-antibodies-IHC-testing-2.jpgAnti-Alpha 1 microglobulin/AMBP Antibody Picoband® IHC analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3).<br>Alpha 1 microglobulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha 1 microglobulin Antibody (A02419-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02419-3-Alpha-1-microglobulin-primary-antibodies-IHC-testing-3.jpgAnti-Alpha 1 microglobulin/AMBP Antibody Picoband® IHC analysis of Alpha 1 microglobulin using anti-Alpha 1 microglobulin antibody (A02419-3).<br>Alpha 1 microglobulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Alpha 1 microglobulin Antibody (A02419-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tmem16a-picoband-trade-antibody-a00713-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00713-tmem16a-primary-antibodies-wb-testing-1.jpgAnti-TMEM16A/ANO1 Antibody Picoband®Western blot analysis of TMEM16A using anti-TMEM16A antibody (A00713). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates, <br>
Lane 2: human Hacat whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human U251 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM16A antigen affinity purified polyclonal antibody (A00713) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM16A at approximately 130 kDa. The expected band size for TMEM16A is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00713-TMEM16A-primary-antibodies-IHC-testing-2.jpgAnti-TMEM16A/ANO1 Antibody Picoband® IHC analysis of TMEM16A using anti-TMEM16A antibody (A00713). <br>TMEM16A was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TMEM16A Antibody (A00713) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00713-TMEM16A-primary-antibodies-FC-testing-3.jpgAnti-TMEM16A/ANO1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-TMEM16A antibody (A00713). <br>Overlay histogram showing A431 cells stained with A00713 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM16A Antibody (A00713,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-aquaporin-5-picoband-trade-antibody-a03085-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03085-Aquaporin-5-primary-antibodies-WB-testing-1.jpgAnti-Aquaporin 5/AQP5 Antibody Picoband® Western blot analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat lung tissue lysates,<br>Lane 2: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 5 antigen affinity purified polyclonal antibody (Catalog # A03085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 5 at approximately 28KD. The expected band size for Aquaporin 5 is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03085-aquaporin-5-primary-antibodies-wb-testing-2.pngAnti-Aquaporin 5/AQP5 Antibody Picoband®Western blot analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: Normal group-rat colon tissue lysates,<br>
Lane 2: Model group-rat colon tissue lysates,<br>
Lane 3: Traditional Chinese medicine treatment (low concetration)-rat colon tissue lysates,<br>
Lane 4: Traditional Chinese medicine treatment (medium concentration)-rat colon tissue lysates,<br>
Lane 5: Traditional Chinese medicine treatment (High concentration)-rat colon tissue lysates,<br>
Lane 6: Western medicine treatment-rat colon tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 5 antigen affinity purified polyclonal antibody (Catalog # A03085) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. The expected band size for Aquaporin 5 is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03085-aquaporin-5-primary-antibodies-wb-testing-3_1.pngAnti-Aquaporin 5/AQP5 Antibody Picoband® Western blot analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: MADB106 cells under normal culture conditions,<br>Lane 2: MADB106 cells treated with agonist, <br>Lane 3: MADB106 cells treated with inhibitor,<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 5 antigen affinity purified polyclonal antibody (Catalog # A03085) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Aquaporin 5 at approximately 23KD. The expected band size for Aquaporin 5 is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03085-Aquaporin-5-primary-antibodies-IHC-testing-2.jpgAnti-Aquaporin 5/AQP5 Antibody Picoband® IHC analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085).<br>Aquaporin 5 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Aquaporin 5 Antibody (A03085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03085-Aquaporin-5-primary-antibodies-IHC-testing-3.jpgAnti-Aquaporin 5/AQP5 Antibody Picoband® IHC analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085).<br>Aquaporin 5 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Aquaporin 5 Antibody (A03085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-amphiregulin-picoband-trade-antibody-a01787-1-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-1-10020_2025_1301_fig2_html.pngAnti-Amphiregulin/Areg Antibody Picoband®FCGR2B were up-regulated in hippocampus of DM mice. A qRT-PCR was performed to detect the expression of ALB, AREG and FCGR2B mRNA expression in hippocampus of mice. B Western blot was conducted to detect the ALB, AREG and FCGR2B protein expression in hippocampus of mice. C IHC assay was employed to examine the ALB, AREG and FCGR2B protein expression in hippocampus of mice. D IF staining was utilized to detect the expression of FCGR2B and NeuN in hippocampus of mice. E Western blot was performed to detect the SHC1 protein expression in hippocampus of mice. F IF staining was performed to detect the expression of SHC1 and NeuN in hippocampus of mice. G Western blot was used to detect the p-PI3K and p-AKT protein expression in hippocampus of mice. *** P < 0.001 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01301-7'>40537751</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-1-amphiregulin-primary-antibodies-wb-testing-1.jpgAnti-Amphiregulin/Areg Antibody Picoband®Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: rat spleen tissue lysates, <br>
Lane 4: rat PC-12 whole cell lysates, <br>
Lane 5: mouse brain tissue lysates, <br>
Lane 6: mouse thymus tissue lysates, <br>
Lane 7: mouse spleen tissue lysates, <br>
Lane 8: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (A01787-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 45 kDa. The expected band size for Amphiregulin is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-1-amphiregulin-primary-antibodies-ihc-testing-1.jpgAnti-Amphiregulin/Areg Antibody Picoband®IHC analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). <br>
Amphiregulin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Amphiregulin Antibody (A01787-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-1-amphiregulin-primary-antibodies-ihc-testing-2_1.jpgAnti-Amphiregulin/Areg Antibody Picoband®IHC analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). <br>
Amphiregulin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Amphiregulin Antibody (A01787-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-1-amphiregulin-primary-antibodies-fcm-testing-3.jpgAnti-Amphiregulin/Areg Antibody Picoband®Flow Cytometry analysis of PC-12 cells using anti-Amphiregulin antibody (A01787-1). <br>
Overlay histogram showing PC-12 cells stained with A01787-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Amphiregulin Antibody (A01787-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-btc-picoband-trade-antibody-a02171-3-boster.html2026-03-24T05:20:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02171-3-btc-primary-antibodies-wb-testing-1_1.jpgAnti-BTC Antibody Picoband®Western blot analysis of BTC using anti-BTC antibody (A02171-3). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human T47D whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: human HEK293 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTC antigen affinity purified polyclonal antibody (A02171-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BTC at approximately 26 kDa. The expected band size for BTC is at 20 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-cacna2d2-picoband-trade-antibody-a01560-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01560-1-cacna2d2-primary-antibodies-wb-testing-1.jpgAnti-CACNA2D2 Antibody Picoband® Western blot analysis of CACNA2D2 using anti-CACNA2D2 antibody (A01560-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human MCF-7 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D2 antigen affinity purified polyclonal antibody (Catalog # A01560-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACNA2D2 at approximately 130KD. The expected band size for CACNA2D2 is at 130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01560-1-CACNA2D2-primary-antibodies-FC-testing-2.jpgAnti-CACNA2D2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-CACNA2D2 antibody (A01560-1).<br>Overlay histogram showing A549 cells stained with A01560-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CACNA2D2 Antibody (A01560-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.<br>
https://www.bosterbio.com/products/primary-antibodies/anti-calbindin-picoband-trade-antibody-a03047-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03047-calbindin-primary-antibodies-wb-testing-1.jpgAnti-Calbindin/CALB1 Antibody Picoband® Western blot analysis of Calbindin using anti-Calbindin antibody (A03047). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: mouse brain tissue lysates,<br>
Lane 4: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody (Catalog # A03047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calbindin at approximately 30 kDa. The expected band size for Calbindin is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03047-calb1-primary-antibodies-ihc-testing-5.jpgAnti-Calbindin/CALB1 Antibody Picoband®IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047). <br>Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin/CALB1 Antibody (A03047) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-2.jpgAnti-Calbindin/CALB1 Antibody Picoband® IHC analysis of Calbindin using anti-Calbindin antibody (A03047).<br>Calbindin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-3.jpgAnti-Calbindin/CALB1 Antibody Picoband® IHC analysis of Calbindin using anti-Calbindin antibody (A03047).<br>Calbindin was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03047-Calbindin-primary-antibodies-IHC-testing-4.jpgAnti-Calbindin/CALB1 Antibody Picoband® IHC analysis of Calbindin using anti-Calbindin antibody (A03047).
<br>Calbindin was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03047-Calbindin-primary-antibodies-FC-testing-5.jpgAnti-Calbindin/CALB1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Calbindin antibody (A03047). <br>
Overlay histogram showing A431 cells stained with A03047 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (A03047,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03047-calbindin-primary-antibodies-if-testing-6.jpgAnti-Calbindin/CALB1 Antibody Picoband® IF analysis of Calbindin using anti-Calbindin antibody (A03047). <br>
Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03047-calbindin-primary-antibodies-if-testing-7.jpgAnti-Calbindin/CALB1 Antibody Picoband® IF analysis of Calbindin using anti-Calbindin antibody (A03047).<br>
Calbindin was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-caspase-2-picoband-trade-antibody-a02384-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02384-1-Caspase-2-primary-antibodies-WB-testing-1.jpgAnti-Caspase-2/CASP2 Antibody Picoband® Western blot analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human PANC-1 whole cell lysate,<br>Lane 2: human 22RV1 whole cell lysate,<br>Lane 3: human SGC-7901 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-2 antigen affinity purified polyclonal antibody (Catalog # A02384-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-2 at approximately 51KD. The expected band size for Caspase-2 is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02384-1-Caspase-2-primary-antibodies-IHC-testing-2.jpgAnti-Caspase-2/CASP2 Antibody Picoband® IHC analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1).<br>Caspase-2 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-2 Antibody (A02384-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02384-1-Caspase-2-primary-antibodies-IHC-testing-3.jpgAnti-Caspase-2/CASP2 Antibody Picoband® IHC analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1).<br>Caspase-2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-2 Antibody (A02384-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02384-1-Caspase-2-primary-antibodies-IHC-testing-4.jpgAnti-Caspase-2/CASP2 Antibody Picoband® IHC analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1).<br>Caspase-2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-2 Antibody (A02384-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02384-1-Caspase-2-primary-antibodies-IHC-testing-5.jpgAnti-Caspase-2/CASP2 Antibody Picoband® IHC analysis of Caspase-2 using anti-Caspase-2 antibody (A02384-1).<br>Caspase-2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-2 Antibody (A02384-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-ccl2-picoband-trade-antibody-a00056-4-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00056-4-CCL2-primary-antibodies-WB-testing-1.jpgAnti-CCL2 Antibody Picoband® Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat pancreas tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ccl6-picoband-trade-antibody-a30384-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30384-ccl6-primary-antibodies-wb-testing-1.jpgAnti-CCL6 Antibody Picoband® Western blot analysis of CCL6 using anti-CCL6 antibody (A30384). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br>Lane 1: recombinant mouse CCL6 protein 1ng. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL6 antigen affinity purified polyclonal antibody (Catalog # A30384) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL6 at approximately 11KD. The expected band size for CCL6 is at 11KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/3/A30384-CCL6-primary-antibodies-IHC-testing-2.jpgAnti-CCL6 Antibody Picoband® IHC analysis of CCL6 using anti-CCL6 antibody (A30384).<br>CCL6 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL6 Antibody (A30384) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-ccl8-picoband-trade-antibody-a03237-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03237-1-CCL8-primary-antibodies-IHC-testing-1.jpgAnti-CCL8 Antibody IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).<br>CCL8 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03237-1-CCL8-primary-antibodies-IHC-testing-2.jpgAnti-CCL8 Antibody IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).<br>CCL8 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03237-1-CCL8-primary-antibodies-IHC-testing-3.jpgAnti-CCL8 Antibody IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).<br>CCL8 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03237-1-CCL8-primary-antibodies-IHC-testing-4.jpgAnti-CCL8 Antibody IHC analysis of CCL8 using anti-CCL8 antibody (A03237-1).
<br>CCL8 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCL8 Antibody (A03237-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-ccl16-picoband-trade-antibody-a07545-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07545-1-CCL16-primary-antibodies-WB-testing-1.jpgAnti-CCL16 Antibody Picoband® Western blot analysis of CCL16 using anti-CCL16 antibody (A07545-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human A431 whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL16 antigen affinity purified polyclonal antibody (Catalog # A07545-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL16 at approximately 18KD. The expected band size for CCL16 is at 14KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cd1b-picoband-trade-antibody-a02158-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02158-1-cd1b-primary-antibodies-wb-testing-1.jpgAnti-CD1b Antibody Picoband® Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human PC-3 whole cell lysate,<br>Lane 3: human SW620 whole cell lysate,<br>Lane 4: human THP-1 whole cell lysate,<br>Lane 5: human MDA-MB-231 whole cell lysate,<br>Lane 6: human K562 whole cell lysate,<br>Lane 7: human A431 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02158-1-cd1b-primary-antibodies-ihc-testing-2.jpgAnti-CD1b Antibody Picoband® IHC analysis of CD1b using anti-CD1b antibody (A02158-1).<br>CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD1b Antibody (A02158-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02158-1-cd1b-primary-antibodies-ihc-testing-3.jpgAnti-CD1b Antibody Picoband® IHC analysis of CD1b using anti-CD1b antibody (A02158-1).
<br>CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD1b Antibody (A02158-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-cd72-picoband-trade-antibody-a09292-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09292-1-cd72-primary-antibodies-wb-testing-1.jpgAnti-CD72 Antibody Picoband® Western blot analysis of CD72 using anti-CD72 antibody (A09292-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A375 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD72 antigen affinity purified polyclonal antibody (Catalog # A09292-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD72 at approximately 45KD. The expected band size for CD72 is at 40KD.
https://www.bosterbio.com/products/primary-antibodies/anti-p-cadherin-picoband-trade-antibody-a03353-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-1-cdh3-primary-antibodies-fcm-testing-3.jpgAnti-P-Cadherin-3 CDH3 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-P cadherin/CDH3 antibody (A03353-1). <br>Overlay histogram showing A431 cells stained with A03353-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P cadherin/CDH3 Antibody (A03353-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-1-cdh3-primary-antibodies-wb-testing-1.jpgAnti-P-Cadherin-3 CDH3 Antibody Picoband® Western blot analysis of P cadherin/CDH3 using anti-P cadherin/CDH3 antibody (A03353-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P cadherin/CDH3 antigen affinity purified polyclonal antibody (Catalog # A03353-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P cadherin/CDH3 at approximately 120 kDa. The expected band size for P cadherin/CDH3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-1-cdh3-primary-antibodies-if-testing-2.jpgAnti-P-Cadherin-3 CDH3 Antibody Picoband® IF analysis of P cadherin/CDH3 using anti-P cadherin/CDH3 antibody (A03353-1). <br>
P cadherin/CDH3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-P cadherin/CDH3 Antibody (A03353-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cfp-picoband-trade-antibody-a00852-2-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00852-2-cfp-primary-antibodies-wb-testing-1.jpgAnti-CFP Antibody Picoband® Western blot analysis of CFP using anti-CFP antibody (A00852-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human U-937 whole cell lysate,<br>Lane 3: rat brain tissue lysates,<br>Lane 4: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFP antigen affinity purified polyclonal antibody (Catalog # A00852-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFP at approximately 51KD. The expected band size for CFP is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-2.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).<br>CFP was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-3.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).<br>CFP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-4.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).<br>CFP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-5.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).<br>CFP was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-6.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).<br>CFP was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00852-2-CFP-primary-antibodies-IHC-testing-7.jpgAnti-CFP Antibody Picoband® IHC analysis of CFP using anti-CFP antibody (A00852-2).
<br>CFP was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CFP Antibody (A00852-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00852-2-8.pngAnti-CFP Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-CFP antibody (A00852-2). <br> Overlay histogram showing THP-1 cells stained with A00852-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CFP Antibody (A00852-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cic-picoband-trade-antibody-a00385-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00385-1-CIC-primary-antibodies-IHC-testing-1.jpgAnti-CIC Antibody IHC analysis of CIC using anti-CIC antibody (A00385-1).<br>CIC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CIC Antibody (A00385-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00385-1-CIC-primary-antibodies-IHC-testing-2.jpgAnti-CIC Antibody IHC analysis of CIC using anti-CIC antibody (A00385-1).<br>CIC was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CIC Antibody (A00385-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00385-1-3.jpgAnti-CIC Antibody IHC analysis of CIC using anti-CIC antibody (A00385-1). <br> CIC was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CIC Antibody (A00385-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00385-1-4.jpgAnti-CIC Antibody IHC analysis of CIC using anti-CIC antibody (A00385-1). <br> CIC was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CIC Antibody (A00385-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00385-1-cic-primary-antibodies-ihc-testing-5.jpgAnti-CIC AntibodyIHC analysis of CIC using anti-CIC antibody (A00385-1). <br>CIC was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CIC Antibody (A00385-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-creatine-kinase-mm-picoband-trade-antibody-a03452-1-boster.html2026-03-24T05:20:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03452-1-creatine-kinase-mm-primary-antibodies-wb-testing-1.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband® Western blot analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysate,<br>Lane 2: human CNE whole cell lysate,<br>Lane 3: human COLO-320 whole cell lysate,<br>Lane 4: rat heart tissue lysates,<br>Lane 5: rat lung tissue lysates,<br>Lane 6: mouse heart tissue lysates,<br>Lane 7: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Creatine Kinase MM antigen affinity purified polyclonal antibody (Catalog # A03452-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Creatine Kinase MM at approximately 43KD. The expected band size for Creatine Kinase MM is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03452-1-creatine-kinase-mm-primary-antibodies-wb-testing-1_1.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband®Western blot analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat heart tissue lysates, <br>
Lane 2: rat skeletal muscle tissue lysates, <br>
Lane 3: mouse heart tissue lysates, <br>
Lane 4: mouse skeletal muscle tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Creatine Kinase MM antigen affinity purified polyclonal antibody (A03452-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Creatine Kinase MM at approximately 43 kDa. The expected band size for Creatine Kinase MM is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03452-1-ckm-primary-antibodies-ihc-testing-4.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband®IHC analysis of CKM using anti-CKM antibody (A03452-1). <br>CKM was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKM Antibody (A03452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03452-1-Creatine-Kinase-MM-primary-antibodies-IHC-testing-2.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband® IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).<br>Creatine Kinase MM was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03452-1-Creatine-Kinase-MM-primary-antibodies-IHC-testing-3.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband® IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).<br>Creatine Kinase MM was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03452-1-creatine-kinase-mm-primary-antibodies-ihc-testing-4.jpgAnti-Creatine Kinase MM/CKM Antibody Picoband® IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). <br> Creatine Kinase MM was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-comp-picoband-trade-antibody-a02443-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02443-1-comp-primary-antibodies-wb-testing-1.jpgAnti-COMP Antibody Picoband® Western blot analysis of COMP using anti-COMP antibody (A02443-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat cartilage tissue lysates,<br>
Lane 6: mouse cartilage tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMP antigen affinity purified polyclonal antibody (Catalog # A02443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMP at approximately 110 kDa. The expected band size for COMP is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02443-1-comp-primary-antibodies-ihc-testing-2.jpgAnti-COMP Antibody Picoband® IHC analysis of COMP using anti-COMP antibody (A02443-1). <br>
COMP was detected in a paraffin-embedded section of mouse knee-joint tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COMP Antibody (A02443-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02443-1-comp-primary-antibodies-ihc-testing-3.jpgAnti-COMP Antibody Picoband® IHC analysis of COMP using anti-COMP antibody (A02443-1). <br>
COMP was detected in a paraffin-embedded section of rat knee-joint tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COMP Antibody (A02443-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-comt-antibody-a00464-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00464-COMT-primary-antibodies-WB-testing-1.jpgAnti-COMT Antibody Picoband® Western blot analysis of COMT using anti-COMT antibody (A00464).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: mouse liver tissue lysates,<br>Lane 2: mouse stomach tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody (Catalog # A00464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMT at approximately 25 and 29KD. The expected band size for COMT is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-c-reactive-protein-picoband-trade-antibody-a00249-4-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00249-4-crp-primary-antibodies-ihc-testing-3.jpgAnti-C Reactive Protein/CRP Antibody Picoband®IHC analysis of CRP using anti-CRP antibody (A00249-4). <br>CRP was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRP Antibody (A00249-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00249-4-crp-primary-antibodies-wb-testing-1.jpgAnti-C Reactive Protein/CRP Antibody Picoband® Western blot analysis of CRP using anti-CRP antibody (A00249-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br>
Lane 1: recombinant human CRP protein 10 ng,<br>
Lane 2: recombinant human CRP protein 5 ng.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRP antigen affinity purified polyclonal antibody (Catalog # A00249-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRP at approximately 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00249-4-crp-primary-antibodies-ihc-testing-2.jpgAnti-C Reactive Protein/CRP Antibody Picoband® IHC analysis of CRP using anti-CRP antibody (A00249-4). <br>
CRP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRP Antibody (A00249-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-csf1-picoband-trade-antibody-a00620-3-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00620-3-csf1-primary-antibodies-wb-testing-1.jpgAnti-CSF1 Antibody Picoband® Western blot analysis of CSF1 using anti-CSF1 antibody (A00620-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human 293T whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 antigen affinity purified polyclonal antibody (Catalog # A00620-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1 at approximately 60KD. The expected band size for CSF1 is at 60KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cortactin-picoband-trade-antibody-a01253-1-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-WB-testing-1.jpgAnti-Cortactin/CTTN Antibody Picoband® Western blot analysis of cortactin using anti-cortactin antibody (A01253-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A431 whole cell lysate,<br>Lane 2: human U-87MG whole cell lysate,<br>Lane 3: human A549 whole cell lysate,<br>Lane 4: human PC-3 whole cell lysate,<br>Lane 5: human Hela whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cortactin antigen affinity purified polyclonal antibody (Catalog # A01253-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cortactin at approximately 85KD. The expected band size for cortactin is at 61KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-10.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-2.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-20.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-3.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-4.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-5.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-6.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-7.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-8.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01253-1-cortactin-primary-antibodies-IHC-testing-9.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-11.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-12.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-13.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-14.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-15.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-16.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-ihc-testing-17.jpgAnti-Cortactin/CTTN Antibody Picoband® IHC analysis of cortactin using anti-cortactin antibody (A01253-1). <br> cortactin was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-fc-testing-18.jpgAnti-Cortactin/CTTN Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-cortactin antibody (A01253-1). <br> Overlay histogram showing A549 cells stained with A01253-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-cortactin Antibody (A01253-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01253-1-cortactin-primary-antibodies-if-testing-19.jpgAnti-Cortactin/CTTN Antibody Picoband® IF analysis of cortactin using anti-cortactin antibody (A01253-1).<br>cortactin was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-cortactin Antibody (A01253-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cul2-picoband-trade-antibody-a02986-3-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02986-3-cul2-primary-antibodies-wb-testing-1.jpgAnti-CUL2 Antibody Picoband® Western blot analysis of CUL2 using anti-CUL2 antibody (A02986-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat RH35 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL2 antigen affinity purified polyclonal antibody (Catalog # A02986-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CUL2 at approximately 87 kDa. The expected band size for CUL2 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02986-3-cul2-primary-antibodies-fcm-testing-2_1.jpgAnti-CUL2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CUL2 antibody (A02986-3). <br>
Overlay histogram showing PC-3 cells stained with A02986-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CUL2 Antibody (A02986-3, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cxcl12-picoband-trade-antibody-a00053-2-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-ihc-testing-1.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
<br>CXCL12 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig2.jpgAnti-CXCL12 AntibodyFITC-labeled SDF-1-loaded MBs by confocal laser scanning microscopy. Notes: ( A ) Green fluorescence was not found in unloaded MBs. ( B ) FITC-MB SDF-1 showed bright green fluorescence when attached to the MBs. Abbreviations: FITC, fluorescein isothiocyanate; SDF-1, stromal cell-derived factor-1; MBs, microbubbles; FITC-MB SDF-1 , FITC-labeled SDF-1-loaded microbubbles.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig3.jpgAnti-CXCL12 AntibodyDetermination of bioactivity by cell migration assay. Notes: ( A ) Determination of bioactivity based on a cell migration assay. The number of migrated MSCs increased in the SDF-1 group and the MB SDF-1 group compared with the control group. Preincubation with AMD3100 inhibited the migration. The white bars in ( A ) indicate 200 μm. ( B ) The histogram of the number of migrated cells in each group. The asterisk indicates a significant difference compared to the control group; * P <0.05 ( P -value of 9.99×10 −15 , SDF-1 versus control; P value of 3.66×10 −14 , MB SDF-1 versus control). The hash symbol indicates a significant difference compared to the same treatment without AMD3100; # P <0.05 ( P -value of 5.00×10 −7 , SDF-1 + AMD3100 versus control + AMD3100; P -value of 1.92×10 −5 , MB SDF-1 + AMD3100 versus control + AMD3100). Abbreviations: MSCs, mesenchymal stem cells; SDF-1, stromal cell-derived factor-1; MB SDF-1 , SDF-1-loaded microbubbles.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig4.jpgAnti-CXCL12 AntibodyIn vivo imaging and ultrasound-targeted microbubble destruction. Notes: ( A ) At a low mechanical index, the renal parenchyma apparently improved after MB SDF-1 infusion, compared with adjacent tissues. After 4 seconds of MBD at a high mechanical index ( B ), the MBs in the renal parenchyma almost disappeared, showing a low acoustic intensity ( C ). ( D – F ) Within 3–4 seconds, the MBs gradually replenished until complete recovery. ( G ) The rectangular region of interest was positioned in the renal parenchyma (red) and the adjacent tissue at the same depth (blue). ( H ) The derived time–intensity curve indicated a sudden echo intensity decrease during MBD and a gradual ascending recovery. Abbreviations: MB SDF-1 , stromal cell-derived factor-1-loaded microbubbles; MBD, microbubble destruction; MBs, microbubbles.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig6.jpgAnti-CXCL12 AntibodyTarget release in kidney under CLSM. Notes: No fluorescence was found in the control kidney (upper panel in [ A ]). After UTMD with FITC-MB SDF-1 , sporadic green fluorescence in the renal interstitium and around the small vessels was observed under a CLSM (lower panel in [ A ]). ( B ) A significantly higher mean gray-scale value in the target kidney (left kidney) compared to any other major organs; * P <0.05 ( P -value of 9.85×10 −10 versus heart, 7.57×10 −10 versus lung, 2.34×10 −10 versus liver, 5.78×10 −10 versus spleen, and 4.59×10 −10 versus right kidney). Abbreviations: CLSM, confocal laser scanning microscope; UTMD, ultrasound-targeted microbubble destruction; FITC-MB SDF-1 , fluorescein isothiocyanate-labeled stromal cell-derived factor-1-loaded microbubbles; DAPI, 4′,6-diamidino-2-phenylindole.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig7.jpgAnti-CXCL12 AntibodySDF-1 expression by immunohistochemistry. Notes: The immunohistochemistry for the renal expression of SDF-1 significantly differed between normal rats and early DN rats. Positive expression was found in DN rats, compared with negative expression in normal rats. The positive expression was brown in the cytoplasm. Abbreviations: SDF-1, stromal cell-derived factor-1; DN, diabetic nephropathy.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-ijn-9-5639fig8.jpgAnti-CXCL12 AntibodyIn vivo detection of implanted exogenous MSCs. Notes: Number of implanted exogenous MSCs in normal and DN kidneys 24 hours after group treatments. ( A ) The differences of implanted MSCs among groups under a confocal laser scanning microscope. Exogenous MSCs were labeled with GFP and displayed green signals. In normal rats, GFP-labeled MSCs were only occasionally detected in all three groups. In DN rats, GFP-labeled MSCs were rare in control group. UTMD increased the exogenous MSCs and UTMD + SDF-1 greatly improved the homing of exogenous MSCs. The white bar indicates 50 μm. ( B ) The histogram comparison of the GFP-labeled MSCs among groups, * P <0.05 ( P -value of 4.93×10 −6 , DN + UTMD versus DN + control; P -value of 4.15×10 −9 , DN + UTMD + SDF-1 versus DN + control) and # P <0.05 ( P -value of 3.02×10 −5 , DN + UTMD + SDF-1 versus DN + UTMD). Abbreviations: MSCs, mesenchymal stem cells; DN, diabetic nephropathy; GFP, green fluorescent protein; UTMD, ultrasound-targeted microbubble destruction; SDF-1, stromal cell-derived factor-1; DAPI, 4′,6-diamidino-2-phenylindole.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4263441/&orig_host=www.ncbi.nlm.nih.gov'>25516709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00053-2-CXCL12-primary-antibodies-IHC-testing-2.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).<br>CXCL12 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-ihc-testing-3.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-ihc-testing-4.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-if-testing-7.jpgAnti-CXCL12 Antibody IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of human hepatitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-5.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br> CXCL12 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-if-testing-8.jpgAnti-CXCL12 Antibody IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-if-testing-9.jpgAnti-CXCL12 Antibody IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-if-testing-10.jpgAnti-CXCL12 Antibody IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2). <br>
CXCL12 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00053-2-cxcl12-primary-antibodies-ihc-testing-6_1.jpgAnti-CXCL12 Antibody IHC analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).<br>CXCL12 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-ddr2-antibody-a01698-1-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01698-1-ddr2-primary-antibodies-wb-testing-1_1.jpgAnti-DDR2 Antibody Picoband® Western blot analysis of DDR2 using anti-DDR2 antibody (A01698-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HT1080 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: rat C6 whole cell lysates,<br>
Lane 5: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # A01698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120 kDa. The expected band size for DDR2 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01698-1-ddr2-primary-antibodies-ihc-testing-1.jpgAnti-DDR2 Antibody Picoband®IHC analysis of DDR2 using anti-DDR2 antibody (A01698-1). <br>
DDR2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDR2 Antibody (A01698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01698-1-ddr2-primary-antibodies-if-testing-2.jpgAnti-DDR2 Antibody Picoband® IF analysis of DDR2 using anti-DDR2 antibody (A01698-1). <br>
DDR2 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDR2 Antibody (A01698-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01698-1-ddr2-primary-antibodies-fcm-testing-3.jpgAnti-DDR2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-DDR2 antibody (A01698-1). <br>
Overlay histogram showing U87 cells stained with A01698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDR2 Antibody (A01698-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ddt-picoband-trade-antibody-a01354-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01354-ddt-primary-antibodies-wb-testing-1_1.jpgAnti-DDT Antibody Picoband® Western blot analysis of DDT using anti-DDT antibody (A01354). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human liver tissue lysates,<br>
Lane 2: rat liver tissue lysates,<br>
Lane 3: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # A01354) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13 kDa. The expected band size for DDT is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01354-ddt-primary-antibodies-ihc-testing-2.jpgAnti-DDT Antibody Picoband® IHC analysis of DDT using anti-DDT antibody (A01354). <br>
DDT was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDT Antibody (A01354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01354-ddt-primary-antibodies-ihc-testing-3.jpgAnti-DDT Antibody Picoband® IHC analysis of DDT using anti-DDT antibody (A01354). <br>
DDT was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDT Antibody (A01354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01354-ddt-primary-antibodies-ihc-testing-4.jpgAnti-DDT Antibody Picoband® IHC analysis of DDT using anti-DDT antibody (A01354). <br>
DDT was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDT Antibody (A01354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-dkk1-picoband-trade-antibody-a00632-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00632-DKK1-primary-antibodies-WB-testing-1.jpgAnti-DKK1 Antibody Picoband® Western blot analysis of DKK1 using anti-DKK1 antibody (A00632). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br>Lane 1: recombinant human DKK1 protein 1ng. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # A00632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 38KD. The expected band size for DKK1 is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00632-13659_2024_446_fig4_html.pngAnti-DKK1 Antibody Picoband®Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and DKK1 of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s13659-024-00446-y'>38656633</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-elavl2-hub-picoband-trade-antibody-a06194-2-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06194-2-ELAVL2-HuB-primary-antibodies-WB-testing-1.jpgAnti-ELAVL2 HuB Antibody Picoband® Western blot analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human T-47D whole cell lysate,<br>Lane 2: human A549 whole cell lysate,<br>Lane 3: human Caco-2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL2 HuB antigen affinity purified polyclonal antibody (Catalog # A06194-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL2 HuB at approximately 45KD. The expected band size for ELAVL2 HuB is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06194-2-ELAVL2-HuB-primary-antibodies-IHC-testing-2.jpgAnti-ELAVL2 HuB Antibody Picoband® IHC analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2).<br>ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL2 HuB Antibody (A06194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06194-2-ELAVL2-HuB-primary-antibodies-IHC-testing-3.jpgAnti-ELAVL2 HuB Antibody Picoband® IHC analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2).<br>ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL2 HuB Antibody (A06194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06194-2-ELAVL2-HuB-primary-antibodies-IHC-testing-4.jpgAnti-ELAVL2 HuB Antibody Picoband® IHC analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2).<br>ELAVL2 HuB was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL2 HuB Antibody (A06194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06194-2-ELAVL2-HuB-primary-antibodies-IHC-testing-5.jpgAnti-ELAVL2 HuB Antibody Picoband® IHC analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2).<br>ELAVL2 HuB was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL2 HuB Antibody (A06194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-eml4-picoband-trade-antibody-a00930-1-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-wb-testing-1_1.jpgAnti-EML4 Antibody Picoband®Western blot analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EML4 antigen affinity purified polyclonal antibody (A00930-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EML4 at approximately 120 kDa. The expected band size for EML4 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-ihc-testing-1.jpgAnti-EML4 Antibody Picoband®IHC analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-ihc-testing-2.jpgAnti-EML4 Antibody Picoband®IHC analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-ihc-testing-3.jpgAnti-EML4 Antibody Picoband®IHC analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-ihc-testing-4.jpgAnti-EML4 Antibody Picoband®IHC analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-ihc-testing-5.jpgAnti-EML4 Antibody Picoband®IHC analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-if-testing-1.jpgAnti-EML4 Antibody Picoband®IF analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-if-testing-2.jpgAnti-EML4 Antibody Picoband®IF analysis of EML4 using anti-EML4 antibody (A00930-1). <br>
EML4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EML4 Antibody (A00930-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00930-1-eml4-primary-antibodies-fcm-testing-1.jpgAnti-EML4 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-EML4 antibody (A00930-1). <br>
Overlay histogram showing A549 cells stained with A00930-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EML4 Antibody (A00930-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nse-picoband-trade-antibody-a02930-boster.html2026-03-24T05:20:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-nse-primary-antibodies-wb-testing-1.jpgAnti-NSE/ENO2 Antibody Picoband®Western blot analysis of NSE/ENO2 using anti-NSE/ENO2 antibody (A02930). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human SH-SY5Y whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat skeletal muscle tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse skeletal muscle tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSE/ENO2 antigen affinity purified polyclonal antibody (A02930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NSE/ENO2 at approximately 47 kDa. The expected band size for NSE/ENO2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-eno2-primary-antibodies-ihc-testing-7.jpgAnti-NSE/ENO2 Antibody Picoband®IHC analysis of NSE/ENO2 using anti-NSE/ENO2 antibody (A02930). <br>NSE/ENO2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NSE/ENO2 Antibody (A02930) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-ajtr0011-2925-f3.jpgAnti-NSE/ENO2 Antibody Picoband®Localization of central AGT and AT1 receptors and Blood brain barrier permeability. A. Localization of central AGT and AT1 receptors determined by doublestaining with the antibodies against AGT or AT1 receptors (green) and the antibodies-recognized NSE or GFAP (red). NSE, neuron-specific enolase; GFAP, glial fibrillary acidic protein. B. Blood brain barrier permeability was up-regulated in DM rats (b1), but there was no significant difference in all intervention groups (b2).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-41598_2018_23568_fig7_html.jpgAnti-NSE/ENO2 Antibody Picoband®Effect of exercise on alcohol induced neuronal damage. ( a,b ) Representative western blot analysis showing the levels of neuronal proteins (NeuN and NSC) in different mice groups ( a ). Histogram showing the quantitative estimation of nNOS and NSE proteins after normalization with GAPDH (b). ( c,d ) Representative images showing coronal slices of mice brains stained with cresyl violet (40× magnification) ( c ). Scatter dot plot showing the number of cresyl violet positive cells in different groups of mice ( d ). ( e,f ) Representative images showing Fluoro-Jade C (FJC) staining in brain sections of the different groups of mice (10× magnification). A marked decrease of FJC-stained degenerating neurons (arrows) were observed in CT, EX and AL+EX groups, indicating a lesser degree of neuronal cell death. Brain sections of AL treated mice showing a greater number of FJC-positive neurons (arrows), reflecting increased neuronal cell death ( e ). Scatter dot plot showing the numbers of degenerating neurons in different experimental mice groups ( f ). All the data are represented as mean values ± standard error (SE) in 5 independent experiments. * ,# p < 0.05 considered significant. *p < 0.05 vs. CT and # p < 0.05 vs. AL group. Uncropped blots for a are presented in Supplementary Fig. . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-23568-z'>29581524</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02930-NSE-primary-antibodies-IHC-testing-2.jpgAnti-NSE/ENO2 Antibody Picoband® IHC analysis of NSE using anti-NSE antibody (A02930).<br>NSE was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02930-NSE-primary-antibodies-IHC-testing-3.jpgAnti-NSE/ENO2 Antibody Picoband® IHC analysis of NSE using anti-NSE antibody (A02930).<br>NSE was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02930-NSE-primary-antibodies-IHC-testing-4.jpgAnti-NSE/ENO2 Antibody Picoband® IHC analysis of NSE using anti-NSE antibody (A02930).<br>NSE was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02930-NSE-primary-antibodies-IHC-testing-5.jpgAnti-NSE/ENO2 Antibody Picoband® IHC analysis of NSE using anti-NSE antibody (A02930).<br>NSE was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02930-NSE-primary-antibodies-IHC-testing-6.jpgAnti-NSE/ENO2 Antibody Picoband® IHC analysis of NSE using anti-NSE antibody (A02930).<br>NSE was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-nse-primary-antibodies-if-testing-7.jpgAnti-NSE/ENO2 Antibody Picoband® IF analysis of NSE using anti-NSE antibody (A02930). <br>
NSE was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-NSE Antibody (A02930) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02930-nse-primary-antibodies-fcm-testing-8.pngAnti-NSE/ENO2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-NSE antibody (A02930). <br>Overlay histogram showing A431 cells stained with A02930 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NSE Antibody (A02930, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-xpb-picoband-trade-antibody-a03103-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03103-1-XPB-primary-antibodies-WB-testing-1.jpgAnti-XPB/ERCC3 Antibody Picoband® Western blot analysis of XPB using anti-XPB antibody (A03103-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPB antigen affinity purified polyclonal antibody (Catalog # A03103-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPB at approximately 89KD. The expected band size for XPB is at 89KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fcgr2a-picoband-trade-antibody-a01450-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01450-1-fcgr2a-primary-antibodies-wb-testing-1.jpgAnti-FCGR2A Antibody Picoband® Western blot analysis of FCGR2A using anti-FCGR2A antibody (A01450-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates<br> Lane 2: rat brain tissue lysates<br> Lane 3: mouse Neuro-2a whole cell lysates <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR2A antigen affinity purified polyclonal antibody (Catalog # A01450-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR2A at approximately 40KD. The expected band size for FCGR2A is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-2.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-3.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-4.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-5.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-6.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-7.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01450-1-FCGR2A-primary-antibodies-IHC-testing-8.jpgAnti-FCGR2A Antibody Picoband® IHC analysis of FCGR2A using anti-FCGR2A antibody (A01450-1).<br>FCGR2A was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FCGR2A Antibody (A01450-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01450-1-fcgr2a-primary-antibodies-elisa-testing-10.jpgAnti-FCGR2A Antibody Picoband®
https://www.bosterbio.com/products/primary-antibodies/anti-fgf2-picoband-trade-antibody-a00121-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-1-fgf2-primary-antibodies-wb-testing-1.jpgAnti-FGF2 Antibody Picoband® Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SKOV3 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-1-fgf2-primary-antibodies-ihc-testing-2.jpgAnti-FGF2 Antibody Picoband® IHC analysis of FGF2 using anti-FGF2 antibody (A00121-1). <br>
FGF2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-1-fgf2-primary-antibodies-if-testing-3.jpgAnti-FGF2 Antibody Picoband® IF analysis of FGF2 using anti-FGF2 antibody (A00121-1). <br>
FGF2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGF2 Antibody (A00121-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1133) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-gm130-picoband-trade-antibody-a05865-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-wb-testing-1.jpgAnti-GM130/GOLGA2 Antibody Picoband® Western blot analysis of GM130 using anti-GM130 antibody (A05865-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 3: human U87 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody (Catalog # A05865-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130 kDa. The expected band size for GM130 is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-ihc-testing-2.jpgAnti-GM130/GOLGA2 Antibody Picoband® IHC analysis of GM130 using anti-GM130 antibody (A05865-1).
GM130 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-ihc-testing-3.jpgAnti-GM130/GOLGA2 Antibody Picoband® IHC analysis of GM130 using anti-GM130 antibody (A05865-1).
GM130 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-ihc-testing-4.jpgAnti-GM130/GOLGA2 Antibody Picoband® IHC analysis of GM130 using anti-GM130 antibody (A05865-1).
GM130 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-ihc-testing-5.jpgAnti-GM130/GOLGA2 Antibody Picoband® IHC analysis of GM130 using anti-GM130 antibody (A05865-1). <br>
GM130 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-if-testing-6.jpgAnti-GM130/GOLGA2 Antibody Picoband® IF analysis of GM130 using anti-GM130 antibody (A05865-1). <br>
GM130 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-GM130 Antibody (A05865-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-fc-testing-7.pngAnti-GM130/GOLGA2 Antibody Picoband®7. Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1). <br>Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GM130 Antibody (A05865-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-fc-testing-8.pngAnti-GM130/GOLGA2 Antibody Picoband®8. Flow Cytometry analysis of U20S cells using anti- GM130 antibody (A05865-1). <br>Overlay histogram showing U20S cells stained with A05865-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GM130 Antibody (A05865-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05865-1-gm130-primary-antibodies-ip-testing-9.jpgAnti-GM130/GOLGA2 Antibody Picoband® Immunoprecipitating GM130 in HepG2 whole cell lysate. <br>Western blot analysis of GM130 using anti-GM130 antibody (A05865-1). <br>Lane 1: HepG2 whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-GM130 antibody in HepG2 whole cell lysate, <br>Lane 3: anti-GM130 antibody (2μg) + HepG2 whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for GM130 at approximately 150 kDa. The expected band size for GM130 is at 113 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-golph3-picoband-trade-antibody-a01333-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01333-1-golph3-primary-antibodies-wb-testing-1.jpgAnti-GOLPH3 Antibody Picoband® Western blot analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human 293T whole cell lysates, <br>
Lane 3: human MDA-MB-453 whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: rat lung tissue lysates, <br>
Lane 6: rat testis tissue lysates, <br>
Lane 7: mouse lung tissue lysates, <br>
Lane 8: mouse testis tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH3 antigen affinity purified polyclonal antibody (Catalog # A01333-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH3 at approximately 36 kDa. The expected band size for GOLPH3 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01333-1-golph3-primary-antibodies-ihc-testing-2.jpgAnti-GOLPH3 Antibody Picoband® IHC analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). <br>
GOLPH3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLPH3 Antibody (A01333-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01333-1-golph3-primary-antibodies-ihc-testing-3.jpgAnti-GOLPH3 Antibody Picoband® IHC analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). <br>
GOLPH3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLPH3 Antibody (A01333-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01333-1-golph3-primary-antibodies-ihc-testing-4.jpgAnti-GOLPH3 Antibody Picoband® IHC analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). <br>
GOLPH3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLPH3 Antibody (A01333-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01333-1-golph3-primary-antibodies-if-testing-5.jpgAnti-GOLPH3 Antibody Picoband® IF analysis of GOLPH3 using anti-GOLPH3 antibody (A01333-1). <br>
GOLPH3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GOLPH3 Antibody (A01333-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-tim-1-picoband-trade-antibody-a01306-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01306-tim_1-primary-antibodies-wb-testing-1_1.jpgAnti-TIM 1/HAVCR1 Antibody Picoband®Western blot analysis of HAVCR1 using anti-HAVCR1 antibody (A01306). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human CACO-2 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat testis tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAVCR1 antigen affinity purified polyclonal antibody (A01306) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAVCR1 at approximately 55 kDa. The expected band size for HAVCR1 is at 39 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-hmgn2-picoband-trade-antibody-a04839-2-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-fcm-testing-7_1.jpgAnti-HMGN2 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-HMGN2 antibody (A04839-2).<br>Overlay histogram showing Raji cells stained with A04839-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGN2 Antibody (A04839-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-wb-testing-1_1.jpgAnti-HMGN2 Antibody Picoband® Western blot analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGN2 antigen affinity purified polyclonal antibody (Catalog # A04839-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGN2 at approximately 18 kDa. The expected band size for HMGN2 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-ihc-testing-2.jpgAnti-HMGN2 Antibody Picoband® IHC analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
HMGN2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGN2 Antibody (A04839-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-ihc-testing-3.jpgAnti-HMGN2 Antibody Picoband® IHC analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
HMGN2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGN2 Antibody (A04839-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-ihc-testing-4.jpgAnti-HMGN2 Antibody Picoband® IHC analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
HMGN2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGN2 Antibody (A04839-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-ihc-testing-5.jpgAnti-HMGN2 Antibody Picoband® IHC analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
HMGN2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGN2 Antibody (A04839-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04839-2-hmgn2-primary-antibodies-ihc-testing-6.jpgAnti-HMGN2 Antibody Picoband® IF analysis of HMGN2 using anti-HMGN2 antibody (A04839-2). <br>
HMGN2 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HMGN2 Antibody (A04839-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-hsp27-picoband-trade-antibody-a00676-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00676-hsp27-primary-antibodies-wb-testing-1.jpgAnti-Hsp27/Hspb1 Antibody Picoband® Western blot analysis of Hsp27 using anti-Hsp27 antibody (A00676). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat heart tissue lysates, <br>
Lane 2: rat stomach tissue lysates, <br>
Lane 3: mouse heart tissue lysates, <br>
Lane 4: mouse stomach tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp27 antigen affinity purified polyclonal antibody (Catalog # A00676) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp27 at approximately 27 kDa. The expected band size for Hsp27 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00676-Hsp27-primary-antibodies-IHC-testing-2.jpgAnti-Hsp27/Hspb1 Antibody Picoband® IHC analysis of Hsp27 using anti-Hsp27 antibody (A00676).<br>Hsp27 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp27 Antibody (A00676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00676-Hsp27-primary-antibodies-IHC-testing-3.jpgAnti-Hsp27/Hspb1 Antibody Picoband® IHC analysis of Hsp27 using anti-Hsp27 antibody (A00676).<br>Hsp27 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp27 Antibody (A00676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00676-Hsp27-primary-antibodies-IHC-testing-4.jpgAnti-Hsp27/Hspb1 Antibody Picoband® IHC analysis of Hsp27 using anti-Hsp27 antibody (A00676).
<br>Hsp27 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Hsp27 Antibody (A00676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-interferon-gamma-antibody-a00393-3-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-fimmu-16-1529991-g002.jpgAnti-Interferon gamma/IFNG Antibody Picoband®Effects of anti-PD-1 and RSV on T cells and IFN-γ in testicular tissue. Percentages of testicular T cells, including CD4 + T cells (A) and CD8 + T cells (B) , were analyzed by flow cytometry in tumor-bearing mice after the final treatment (n = 4 at least, in each group). The mRNA level of IFN-γ in the testis was analyzed by qRT–qPCR (C) and the protein level of IFN-γ was analyzed by western blot (D) (n = 3 per group). Data are presented as means ± SEM. *P < 0.05 vs. Control group; #P < 0.05 vs. ICI group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1529991/full'>40145083</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-41467_2024_54081_fig3_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®Biologic effects of the fibrillar-transformable TPM1 in vitro. a CLSM image of SKBR-3 cells after incubation with TPM1 nanoparticles (red) and biotinylated PD-1 protein (green). Biotinylated PD-1 was labelled with Alexa Fluor™ 488-conjugated streptavidin. Experiment was independently repeated three times with similar results. Scale bar, 20 μm. b CLSM images of SKBR-3 cells incubated with TPM1 nanoparticles (red) for 4 h after treatment with or without IFN-γ (100 ng/mL) for 24 h. Experiment was independently repeated three times with similar results. Scale bars, 20 μm. c SEM images of SKBR-3 cells incubated with TPM1 nanoparticles (red) for 4 h after treatment with or without IFN-γ (100 ng/mL) for 24 h. Experiment was independently repeated three times with similar results. Rectangle: magnified insert. d CLSM images of SKBR-3 cells transfected with pGIPZ-PD-L1-EGFP plasmid (green) after treatment with TPM1 nanoparticles at different times. Experiment was independently repeated three times with similar results. White arrows indicate aggregation of PD-L1 protein. Scale bars, 5 μm. e CLSM images of SKBR-3 cells, SKBR-3 cells treated with Y-27632, and SKBR-3 cells expressing constitutively active RhoA V14 mutant after incubation with TPM1 nanoparticles (red) for 4 h. Experiment was independently repeated three times with similar results. Scale bars, 20 μm. f Quantitative analysis of relative fluorescence (FL) density of SKBR-3 cells, SKBR-3 cells treated with Y-27632, and SKBR-3 cells expressing constitutively active RhoA V14 mutant after incubation with TPM1 nanoparticles for 4 h. Data are presented as mean ± SD ( n = 13 independent experiments). Statistical analysis was performed using one-way ANOVA with a Tukey post hoc test. g Schematic of the procedure for nanopillar cellular traction force measurement by microscopy. h The cellular force (F) of SKBR-3 cells with or without treatment of TPM1 nanoparticles. Experiment was independently repeated three times with similar results. Source data for ( f , h ) are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54081-9'>39521768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-41467_2024_54081_fig4_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®Immunotherapeutic effects of TPM1 on 4T1 cells in vitro. a Experimental scheme for co-culture of activated CD8 + T cells and 4T1 tumour cells. CD8 + T cells were isolated from the mouse spleen, proliferated with IL-2, and activated with anti-CD3 and anti-CD28 Dynabeads. After activated CD8 + T cells were co-cultured with 4T1 cells, treatment of TPM1 nanoparticles was administrated. b SEM images of 4T1 cells and co-cultured CD8 + T cells after incubation with TPM1 nanoparticles for 4 h. Experiment was independently repeated five times with similar results. Scale bars, 1 μm. c and d The concentrations of IFN-γ ( c ) and GZMB ( d ) secreted by activated CD8 + T cells and in the co-culture supernatants of activated CD8 + T cells with 4T1 cells or 4T1 cells treated with anti-PD-L1 antibody (68 nM; Selleck, A2004), PD-L1-targeted peptides, TPM1 nanoparticles, TPM2 nanoparticles, or TPM3 nanoparticles. Data are presented as mean ± SD ( n = 5 independent experiments). Statistical analysis was performed using ANOVA with a Tukey post hoc test; ns not significant. e Cellular viability of 4T1 cells treated with anti-PD-L1 antibody (68 nM; Selleck, A2004), PD-L1-targeted peptides, and TPM1 nanoparticles, TPM2 nanoparticles and TPM3 nanoparticles after co-culture with or without activated CD8 + T cells for 24 h. Data are presented as mean ± SD ( n = 5 independent experiments). Statistical analysis was performed using one-way ANOVA with a Tukey post hoc test. f CLSM images of live 4T1 cells stained with Calcein-AM after treatments of anti-PD-L1 antibody (68 nM; Selleck, A2004), PD-L1-targeted peptides, and TPM1 nanoparticles, TPM2 nanoparticles and TPM3 nanoparticles after co-culture with or without activated CD8 + T cells. Scale bars, 200 μm. Experiment was independently repeated three times with similar results. Source data for ( c – e ) are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54081-9'>39521768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-12575_2019_112_fig3_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®Cytoprotective effect of AKT1 over-expression on MSCs. a Representative flow cytometry scatter plots depicting the percentage of apoptotic cells in Null-MSCs and AKT1-MSCs group under routine culture condition and IFN-γ stimulation condition. b The percentage of Annexin V-positive cells in each group is indicated in a bar chart ( left ). The ratio of Annexin V-positive cells under IFN-γ stimulation condition relative to untreated controls is indicated in a bar chart ( right ). The data are presented as the means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. c Liver fluorescence images on day1, day7 and day14 after transplanting Null-MSCs or AKT1-MSCs into mice with or without Con A administration. d Relative quantification analysis of Null-MSCs and AKT-MSCs on day7 and day14 in ConA administration group. Fluorescence signals were quantified by ROI analysis using Living Image software 4.2 and normalized to those of Day1 in each group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12575-019-0112-2'>31889917</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-41467_2024_54081_fig6_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®Immunotherapeutic effect of TPM1 nanoparticles in mouse model of LLC pulmonary cancer. a Schematic of tumour inoculation and treatment protocol for mice model of LLC pulmonary cancer. After the establishment of the animal tumour model, mice bearing LLC pulmonary cancer received five different treatments, including intravenous injection of PBS (200 μL per injection), intravenous injections of TPM1 (13 mg/kg per injection), TPM2 (13 mg/kg per injection) or TPM3 nanoparticles (13 mg/kg per injection), or intraperitoneal injection of anti-PD-L1 antibody (5 mg/kg per injection; Selleck, A2004) every two days for nine days. b Tumour growth curves for LLC tumour-bearing mice after the five different treatments. Data are presented as mean ± SD ( n = 5 mice in five independent groups). Statistical analysis was performed using two-way ANOVA calculated statistical significance with a Tukey post hoc test. c Body weights of LLC tumour-bearing mice after the five different treatments. Data are presented as mean ± SD ( n = 5 independent experiments). Statistical significance was calculated using two-way ANOVA with a Tukey post hoc test. ns not significant (two-way ANOVA with a Tukey post hoc test). d – i Flow cytometry analysis of the ratio of CD8 + T cells ( d ) and the ratio of Foxp3 + T cells ( e ) to CD45 + CD3 + CD4 + cells within LLC tumour tissues after the five different treatments, and the ratios of GZMB + T cells ( f ), IFN-γ + T cells ( g ), IL-2 + T cells ( h ), and TNF + T cells ( i ) to CD8 + T cells within LLC tumour tissues after the five different treatments ( n = 6 in five independent groups). Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA with a Tukey post hoc test. ns not significant. j The relative mRNA expressions of GZMB, IFN-γ, IL-2, and TNF genes in LLC tumour tissues in mice bearing LLC pulmonary cancer after the five different treatments determined by quantitative real-time reverse transcription PCR. Data are presented as mean ± SD ( n = 5 mice in five independent groups). Statistical analysis was performed using one-way ANOVA with a Tukey post hoc test. ns not significant. Source data for ( b – j ) are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54081-9'>39521768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-41467_2024_54081_fig7_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®Enhanced anti-cancer activity of TPM1 nanoparticles in mouse model of 4T1 breast cancer via reinvigorating CD8 + T cells. a Representative micrographs of immunohistochemistry and immunofluorescent staining of CD4 and CD8 in tumour tissues from mice bearing 4T1 breast cancer after receiving five different treatments including intravenous injection of PBS (200 μL per injection), intravenous injections of TPM1 (13 mg/kg per injection), TPM2 (13 mg/kg per injection) or TPM3 nanoparticles (13 mg/kg per injection), or intraperitoneal injection of anti-PD-L1 antibody (5 mg/kg per injection; Selleck, A2004) every two days for nine days. Images were representative shown out of 5 independent mice per group. Scale bars, 50 μm. b Flow cytometry analysis of the ratios of CD8 + T cells and CD4 + T cells to CD45 + CD3 + T cells in tumour tissues from mice bearing 4T1 breast cancer after the five different treatments ( n = 6 mice in five independent groups). c Flow cytometry analysis of the ratio of Foxp3 + T cells to CD45 + CD3 + CD4 + T cells in tumour tissues from mice bearing 4T1 breast cancer after the five different treatments ( n = 6 mice in five independent groups). d Flow cytometry analysis of the ratios of GZMB-, IFN-γ-, IL-2-, and TNF-producing CD8 + T cells to CD45 + CD3 + T cells in tumour tissues from mice bearing 4T1 breast cancer after the five different treatments ( n = 6 mice in five independent groups). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54081-9'>39521768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-12575_2019_112_fig4_html.pngAnti-Interferon gamma/IFNG Antibody Picoband®AKT1 over-expression promoted cytokines release by MSCs. a The mRNA expression of IL-4, IL-10, PTGES2, HGF and VEGF in Null-MSCs and AKT1-MSCs were measured by qRT-PCR under routine culture condition and IFN-γ stimulation condition. The gene transcript levels were normalized to those of GAPDH and set to 1 in Null-MSCs untreated group. b ELISA analysis for detecting IL-10, PGE2, VEGF and HGF protein expression in MSCs culture supernatant after IFN-γ stimulation for 24h. The data are presented as the means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12575-019-0112-2'>31889917</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-41598_2017_15533_fig2_html.jpgAnti-Interferon gamma/IFNG Antibody Picoband®Th1/Th2 imbalance and Th17 immune response. ( A ) Immunohistochemistry and average optical density for IFN-γ, F DBP = 5.596 (p = 0.002), F TG = 142.67 (p = 0.000), F DBP*TG = 1.114 (p = 0.351). ( B ) Immunohistochemistry and average optical density for IL-4, F DBP = 0.537 (p = 0.659), F TG = 1.419 (p = 0.24), F DBP*TG = 0.168 (p = 0.917). ( C ) Immunohistochemistry and average optical density for IL-17 F DBP = 6.966 (p = 0.001), F TG = 93.46 (p = 0.000), F DBP*TG = 4.136 (p = 0.011). Magnification = ×40. *p < 0.05, **p < 0.01, compared with the saline group; # p < 0.05, ## p < 0.01, compared with the TG group; & p < 0.05, && p < 0.01, compared with the DBP50 + TG group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-15533-z'>29133889</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-ifng-primary-antibodies-wb-testing-1_1.jpgAnti-Interferon gamma/IFNG Antibody Picoband® Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: rat spleen tissue lysates,<br>
Lane 3: mouse spleen tissue lysates,<br>
Lane 4: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19 kDa. The expected band size for IFNG is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-ifng-primary-antibodies-ihc-testing-2.jpgAnti-Interferon gamma/IFNG Antibody Picoband® IHC analysis of IFNG using anti-IFNG antibody (A00393-3). <br>
IFNG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00393-3-ifng-primary-antibodies-ihc-testing-3.jpgAnti-Interferon gamma/IFNG Antibody Picoband® IHC analysis of IFNG using anti-IFNG antibody (A00393-3). <br>
IFNG was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-igf2r-picoband-trade-antibody-a00951-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00951-1-igf2r-primary-antibodies-wb-testing-1.jpgAnti-Mannose 6 Phosphate Receptor/Igf2r Antibody Picoband® Western blot analysis of Igf2r using anti-Igf2r antibody (A00951-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: rat lung tissue lysates,<br>
Lane 3: rat C6 whole cell lysates,<br>
Lane 4: mouse brain tissue lysates,<br>
Lane 5: mouse lung tissue lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Igf2r antigen affinity purified polyclonal antibody (Catalog # A00951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Igf2r at approximately 274 kDa. The expected band size for Igf2r is at 274 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00951-1-Igf2r-primary-antibodies-IHC-testing-2.jpgAnti-Mannose 6 Phosphate Receptor/Igf2r Antibody Picoband® IHC analysis of Igf2r using anti-Igf2r antibody (A00951-1).
<br>Igf2r was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Igf2r Antibody (A00951-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00951-1-Igf2r-primary-antibodies-IHC-testing-3.jpgAnti-Mannose 6 Phosphate Receptor/Igf2r Antibody Picoband® IHC analysis of Igf2r using anti-Igf2r antibody (A00951-1).
<br>Igf2r was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Igf2r Antibody (A00951-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-il10-picoband-trade-antibody-a00021-2-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-2-il10-primary-antibodies-wb-testing-1.jpgAnti-IL10 Antibody Picoband® Western blot analysis of IL10 using anti-IL10 antibody (A00021-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human U-87MG whole cell lysate,<br>Lane 2: rat testis tissue lysates,<br>Lane 3: mouse testis tissue lysates,<br>Lane 4: mouse SP20 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-2-jcav16p1598g004.jpgAnti-IL10 Antibody Picoband®Different inducers promote polarization of different subtypes of macrophages. a The CCK-8 method was used to detect the survival of M0 macrophages after 24 h of IL-4 intervention. b The CCK-8 method was used to detect the survival of M0 macrophages after 24 h of intervention with TGFβ1. c The CCK-8 method was used to detect the survival of M0 macrophages after LPS intervention for 24 h. d RT-qPCR was used to detect the expression of CD86 mRNA. e RT-qPCR was used to detect the expression of iNOS mRNA. f RT-qPCR was used to detect the expression of CD206 mRNA. g RT-qPCR was used to detect the expression of IL1R2 mRNA. h RT-qPCR was used to detect the expression of CD163 mRNA. i RT-qPCR was used to detect the expression of TGFβ mRNA. j ELISA detects TNF - α levels. k ELISA was used to detect IL-10 levels. l ELISA detects the content of TGFβ1. m The WB results of different interventions on histone expression. f TNF-α protein expression results. f IL-10 protein expression results. f TGFβ1 protein expression results. *p<0.05, **p<0.01, ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11843238/'>39991579</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-2-jcav16p1598g006.jpgAnti-IL10 Antibody Picoband®The effect of M2 subtype macrophages on TGFβ1 related pathway and epithelial mesenchymal transition in gastric cancer cells. a Cell intervention pattern diagram. b RT-qPCR was used to detect the levels of TGFβ mRNA in different intervention groups. c ELISA was used to test the expression of TNF-α in different intervention groups. d ELISA test the expression of IL-10 in different intervention groups. e ELISA was used to test the expression of TGFβ1 in different intervention groups. f The expression results of TGFβ1 protein. g The WB results of different interventions on histone expression. h The expression results of p-Smad2 protein. i The expression results of Smad2 protein. j The expression results of p-Smad3 protein. k The expression results of Smad3 protein. l The expression results of E-cadherin protein. m Results of N-cadherin protein expression. n Results of Vimentin protein expression. o RT-qPCR was used to detect the content of TGFβ mRNA at different intervention times. p ELISA was used to test the expression of TNF-α at different intervention times. q ELISA test the expression of IL-10 at different intervention times. r ELISA was used to test the expression of TGFβ1 at different intervention times. s WB results of protein expression at different intervention times. t The expression results of E-cadherin protein. u The expression results of N-cadherin protein. v Results of Vimentin protein expression. w Fluorescence results of mitochondrial membrane potential at different intervention times. Scale bar=50 μm. *p<0.05, **p<0.01, ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11843238/'>39991579</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-2-jcav16p1598g008.jpgAnti-IL10 Antibody Picoband®The role of gastric cancer cells in transforming macrophages in the TME. a The expression of M1 macrophage marker proteins in different groups. b The expression of M2 macrophage marker proteins in different groups. c Differential gene expression between M0 and gastric cancer cell metabolite intervention group. d Differential gene expression between M2 and gastric cancer cell metabolite intervention group. e Intersection statistics of differentially expressed genes between M2 and gastric cancer cell metabolite intervention group. f Intersection gene enrichment statistics. g RT-qPCR was used to detect the expression of CD86 mRNA. h RT-qPCR was used to detect the expression of iNOS mRNA. i RT-qPCR was used to detect the expression of CD206 mRNA. j RT-qPCR was used to detect the expression of IL1R2 mRNA. k RT-qPCR was used to detect the expression of CD163 mRNA. l RT-qPCR was used to detect the expression of TGFβ mRNA. m ELISA was used to detect TNF-α levels. n ELISA detects IL-10 levels. o ELISA detects the content of TGFβ1. *p<0.05; **p<0.01; ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11843238/'>39991579</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-inppl1-picoband-trade-antibody-a01790-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-wb-testing-1.jpgAnti-INPPL1 Antibody Picoband® Western blot analysis of INPPL1 using anti-INPPL1 antibody (A01790). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPPL1 antigen affinity purified polyclonal antibody (Catalog # A01790) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 150 kDa. The expected band size for INPPL1 is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-fcm-testing-10.jpgAnti-INPPL1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-INPPL1 antibody (A01790). <br>Overlay histogram showing A431 cells stained with A01790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INPPL1 Antibody (A01790,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-2.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-3.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-4.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-5.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-6.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-7.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-ihc-testing-8.jpgAnti-INPPL1 Antibody Picoband® IHC analysis of INPPL1 using anti-INPPL1 antibody (A01790).<br>INPPL1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01790-inppl1-primary-antibodies-if-testing-9.jpgAnti-INPPL1 Antibody Picoband® IF analysis of INPPL1 using anti-INPPL1 antibody (A01790). <br> INPPL1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-INPPL1 Antibody (A01790) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-integrin-alpha-3-picoband-trade-antibody-a02902-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02902-Integrin-alpha-3-primary-antibodies-WB-testing-1.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® Western blot analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysate,<br>Lane 2: human PC-3 whole cell lysate,<br>Lane 3: human A431 whole cell lysate,<br>Lane 4: human placenta tissue lysates,<br>Lane 5: human SHG-44 whole cell lysate,<br>Lane 6: human U20S whole cell lysate,<br>Lane 7: human Hela whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 3 antigen affinity purified polyclonal antibody (Catalog # A02902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 3 at approximately 130KD. The expected band size for Integrin alpha 3 is at 116KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02902-Integrin-alpha-3-primary-antibodies-WB-testing-2.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® Western blot analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse HEPA1-6 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 3 antigen affinity purified polyclonal antibody (Catalog # A02902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 3 at approximately 130KD. The expected band size for Integrin alpha 3 is at 116KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02902-Integrin-alpha-3-primary-antibodies-IHC-testing-3.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902).<br>Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin alpha 3 Antibody (A02902) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02902-Integrin-alpha-3-primary-antibodies-IHC-testing-4.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902).<br>Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin alpha 3 Antibody (A02902) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02902-Integrin-alpha-3-primary-antibodies-IHC-testing-5.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902).<br>Integrin alpha 3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin alpha 3 Antibody (A02902) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-integrin-alpha-3-primary-antibodies-if-testing-6.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IF analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902). <br> Integrin alpha 3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Integrin alpha 3 Antibody (A02902) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-integrin_alpha_3-primary-antibodies-fcm-testing-7.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband®Figure 7 Flow Cytometry analysis of U87 cells using anti-Integrin alpha 3 antibody (A02902). <br>Overlay histogram showing U87 cells stained with A02902 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 3 Antibody (A02902, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kcna1-picoband-trade-antibody-a01813-1-boster.html2026-03-24T05:20:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-wb-testing-1.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® Western blot analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 antigen affinity purified polyclonal antibody (Catalog # A01813-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA1 at approximately 56KD. The expected band size for KCNA1 is at 56KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-ihc-testing-2.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® IHC analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). <br>
KCNA1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KCNA1 Antibody (A01813-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-ihc-testing-3.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® IHC analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). <br>
KCNA1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KCNA1 Antibody (A01813-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-if-testing-4.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® IF analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). <br>
KCNA1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-KCNA1 Antibody (A01813-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-if-testing-5.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® IF analysis of KCNA1 using anti-KCNA1 antibody (A01813-1). <br>
KCNA1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-KCNA1 Antibody (A01813-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01813-1-kcna1-primary-antibodies-fcm-testing-6.pngAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-KCNA1 antibody (A01813-1). <br>Overlay histogram showing Raji cells stained with A01813-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNA1 Antibody (A01813-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kcnh1-picoband-trade-antibody-a01036-3-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01036-3-KCNH1-primary-antibodies-WB-testing-1.jpgAnti-KCNH1 Antibody Picoband® Western blot analysis of KCNH1 using anti-KCNH1 antibody (A01036-3).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: mouse brain tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody (Catalog # A01036-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 160KD. The expected band size for KCNH1 is at 111KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01036-3-kcnh1-primary-antibodies-if-testing-2.jpgAnti-KCNH1 Antibody Picoband® IF analysis of KCNH1 using anti-KCNH1 antibody (A01036-3). <br>
KCNH1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KCNH1 Antibody (A01036-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01036-3-kcnh1-primary-antibodies-fcm-testing-3.jpgAnti-KCNH1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-KCNH1 antibody (A01036-3).<br>Overlay histogram showing A549 cells stained with A01036-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNH1 Antibody (A01036-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kcnn4-antibody-a01936-2-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01936-2-KCNN4-primary-antibodies-WB-testing-1.jpgAnti-KCNN4 Antibody Picoband® Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Caco-2 whole cell lysate,<br>Lane 2: human PC-3 whole cell lysate,<br>Lane 3: human A549 whole cell lysate,<br>Lane 4: human Hela whole cell lysate,<br>Lane 5: rat stomach tissue lysates,<br>Lane 6: rat testis tissue lysates,<br>Lane 7: mouse testis tissue lysates,<br>Lane 8: mouse liver tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.
https://www.bosterbio.com/products/primary-antibodies/anti-lats1-picoband-trade-antibody-a01051-2-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01051-2-lats1-primary-antibodies-wb-testing-1.jpgAnti-LATS1 Antibody Picoband® Western blot analysis of LATS1 using anti-LATS1 antibody (A01051-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LATS1 antigen affinity purified polyclonal antibody (Catalog # A01051-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LATS1 at approximately 150KD. The expected band size for LATS1 is at 127KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01051-2-LATS1-primary-antibodies-IHC-testing-2.jpgAnti-LATS1 Antibody Picoband® IHC analysis of LATS1 1using anti-LATS1 antibody (A01051-2).<br>LATS1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LATS1 Antibody (A01051-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01051-2-LATS1-primary-antibodies-IHC-testing-3.jpgAnti-LATS1 Antibody Picoband® IHC analysis of LATS1 using anti-LATS1 antibody (A01051-2).<br>LATS1 was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LATS1 Antibody (A01051-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01051-2-LATS1-primary-antibodies-IHC-testing-4.jpgAnti-LATS1 Antibody Picoband® IHC analysis of LATS1 using anti-LATS1 antibody (A01051-2).
<br>LATS1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LATS1 Antibody (A01051-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01051-2-5.pngAnti-LATS1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-LATS1 antibody (A01051-2). <br> Overlay histogram showing SiHa cells stained with A01051-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LATS1 Antibody (A01051-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-lman1-antibody-a03628-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-wb-testing-1_1.jpgAnti-LMAN1 Antibody Picoband®Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human 293T whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58 kDa. The expected band size for LMAN1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-wb-testing-2_1.jpgAnti-LMAN1 Antibody Picoband®Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: rat heart tissue lysates, <br>
Lane 5: rat C6 whole cell lysates, <br>
Lane 6: mouse liver tissue lysates, <br>
Lane 7: mouse heart tissue lysates, <br>
Lane 8: mouse heart whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58 kDa. The expected band size for LMAN1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-ihc-testing-3_1.jpgAnti-LMAN1 Antibody Picoband®IHC analysis of LMAN1 using anti-LMAN1 antibody (A03768-1). <br>
LMAN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LMAN1 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-ihc-testing-4_1.jpgAnti-LMAN1 Antibody Picoband®IHC analysis of LMAN1 using anti-LMAN1 antibody (A03768-1). <br>
LMAN1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LMAN1 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-if-testing-5_1.jpgAnti-LMAN1 Antibody Picoband®IF analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). <br>
LMAN1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LMAN1 Antibody (A03628-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-if-testing-1_1.jpgAnti-LMAN1 Antibody Picoband®IF analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). <br>
LMAN1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LMAN1 Antibody (A03628-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03628-1-lman1-primary-antibodies-if-testing-2_1.jpgAnti-LMAN1 Antibody Picoband®IF analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). <br>
LMAN1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LMAN1 Antibody (A03628-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-lrig1-picoband-trade-antibody-a04613-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04613-1-LRIG1-primary-antibodies-WB-testing-1.jpgAnti-LRIG1 Antibody Picoband® Western blot analysis of LRIG1 using anti-LRIG1 antibody (A04613-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG1 antigen affinity purified polyclonal antibody (Catalog # A04613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG1 at approximately 145KD. The expected band size for LRIG1 is at 119KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04613-1-LRIG1-primary-antibodies-IHC-testing-2.jpgAnti-LRIG1 Antibody Picoband® IHC analysis of LRIG1 using anti-LRIG1 antibody (A04613-1).<br>LRIG1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LRIG1 Antibody (A04613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-lumican-picoband-trade-antibody-a01034-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01034-1-Lumican-primary-antibodies-WB-testing-1.jpgAnti-Lumican Antibody Picoband® Western blot analysis of Lumican using anti-Lumican antibody (A01034-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human Caco-2 whole cell lysate,<br>Lane 3: human CCRF-CEM whole cell lysate,<br>Lane 4: human Hela whole cell lysate,<br>Lane 5: human Jurkat whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # A01034-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 58KD. The expected band size for Lumican is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01034-1-lumican-primary-antibodies-wb-testing-2.jpgAnti-Lumican Antibody Picoband® Western blot analysis of Lumican using anti-Lumican antibody (A01034-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse liver tissue lysates,<br>Lane 2: mouse ovary tissue lysates,<br>Lane 3: mouse testis tissue lysates,<br>Lane 4: mouse lung tissue lysates,<br>Lane 5: rat liver tissue lysates,<br>Lane 6: rat ovary tissue lysates,<br>Lane 7: rat testis tissue lysates,<br>Lane 8: rat lung tissue lysates,<br>Lane 9: rat heart tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican antigen affinity purified polyclonal antibody (Catalog # A01034-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lumican at approximately 58KD. The expected band size for Lumican is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01034-1-Lumican-primary-antibodies-IHC-testing-3.jpgAnti-Lumican Antibody Picoband® IHC analysis of Lumican using anti-Lumican antibody (A01034-1).<br>Lumican was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lumican Antibody (A01034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01034-1-Lumican-primary-antibodies-IHC-testing-4.jpgAnti-Lumican Antibody Picoband® IHC analysis of Lumican using anti-Lumican antibody (A01034-1).<br>Lumican was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lumican Antibody (A01034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01034-1-Lumican-primary-antibodies-IHC-testing-5.jpgAnti-Lumican Antibody Picoband® IHC analysis of Lumican using anti-Lumican antibody (A01034-1).<br>Lumican was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lumican Antibody (A01034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01034-1-Lumican-primary-antibodies-IHC-testing-6.jpgAnti-Lumican Antibody Picoband® IHC analysis of Lumican using anti-Lumican antibody (A01034-1).<br>Lumican was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lumican Antibody (A01034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-madcam1-picoband-trade-antibody-a04227-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04227-1-MAdCAM1-primary-antibodies-WB-testing-1.jpgAnti-MAdCAM1 Antibody Picoband® Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (A04227-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human 293T whole cell lysate,<br>Lane 2: human A375 whole cell lysate,<br>Lane 3: human K562 whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAdCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAdCAM1 at approximately 43KD. The expected band size for MAdCAM1 is at 40KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mef2c-picoband-trade-antibody-a01131-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-wb-testing-1.jpgAnti-MEF2C Antibody Picoband® Western blot analysis of MEF2C using anti-MEF2C antibody (A01131-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human COLO320 whole cell lysates, <br>
Lane 3: human DAUDI whole cell lysates, <br>
Lane 4: human U937 whole cell lysates, <br>
Lane 5: rat brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2C antigen affinity purified polyclonal antibody (Catalog # A01131-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEF2C at approximately 50-65KD. The expected band size for MEF2C is at 50-65KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-ihc-testing-5.jpgAnti-MEF2C Antibody Picoband®IHC analysis of MEF2C using anti-MEF2C antibody (A01131-1). <br>MEF2C was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MEF2C Antibody (A01131-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-ihc-testing-2.jpgAnti-MEF2C Antibody Picoband® IHC analysis of MEF2C using anti-MEF2C antibody (A01131-1). <br>
MEF2C was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEF2C Antibody (A01131-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-ihc-testing-3.jpgAnti-MEF2C Antibody Picoband® IHC analysis of MEF2C using anti-MEF2C antibody (A01131-1). <br>
MEF2C was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEF2C Antibody (A01131-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-ihc-testing-4.jpgAnti-MEF2C Antibody Picoband® IHC analysis of MEF2C using anti-MEF2C antibody (A01131-1). <br>
MEF2C was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEF2C Antibody (A01131-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01131-1-mef2c-primary-antibodies-fcm-testing-5.pngAnti-MEF2C Antibody Picoband® Flow Cytometry analysis of HELA cells using anti-MEF2C antibody (A01131-1). <br>Overlay histogram showing HELA cells stained with A01131-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEF2C Antibody (A01131-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mettl3-antibody-a01758-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01758-1-mettl3-primary-antibodies-fcm-testing-2_1.pngAnti-METTL3 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-METTL3 antibody (A01758-1). <br>Overlay histogram showing HEPA1-6 cells stained with A01758-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (A01758-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01758-1-mettl3-primary-antibodies-wb-testing-1.jpgAnti-METTL3 Antibody Picoband® Western blot analysis of METTL3 using anti-METTL3 antibody (A01758-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human Hacat whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat testis tissue lysates, <br>
Lane 7: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody (Catalog # A01758-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL3 at approximately 64 kDa. The expected band size for METTL3 is at 64 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-mib1-mindbomb-picoband-trade-antibody-a01387-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01387-1-mib1-primary-antibodies-wb-testing-1_1.jpgAnti-Mib1/Mindbomb Antibody Picoband® Western blot analysis of Mib1 using anti-Mib1 antibody (A01387-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: rat liver tissue lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse liver tissue lysates,<br>
Lane 5: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mib1 antigen affinity purified polyclonal antibody (Catalog # A01387-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mib1 at approximately 110 kDa. The expected band size for Mib1 is at 110 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-nova1-picoband-trade-antibody-a06087-1-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-wb-testing-1.jpgAnti-Nova1 Antibody Picoband® Western blot analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human U87 whole cell lysates,<br>
Lane 4: human THP-1 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nova1 antigen affinity purified polyclonal antibody (Catalog # A06087-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nova1 at approximately 52-75 kDa. The expected band size for Nova1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-2.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-11.jpgAnti-Nova1 Antibody Picoband®IHC analysis of NOVA1 using anti-NOVA1 antibody (A06087-1). <br>NOVA1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOVA1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-3.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-4.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-5_1.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-6.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-7.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-8.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-9_1.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1). <br>
Nova1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-ihc-testing-10.jpgAnti-Nova1 Antibody Picoband® IHC analysis of Nova1 using anti-Nova1 antibody (A06087-1).<br> Nova1 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-if-testing-11.jpgAnti-Nova1 Antibody Picoband® IF analysis of Nova1 using anti-Nova1 antibody (A06087-1) and anti-Beta Tubulin antibody (M01857-3).<br>
Nova1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-Nova1 Antibody (A06087-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-if-testing-12.jpgAnti-Nova1 Antibody Picoband® IF analysis of Nova1 using anti-Nova1 antibody (A06087-1).<br> Nova1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Nova1 Antibody (A06087-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06087-1-nova1-primary-antibodies-fcm-testing-13.jpgAnti-Nova1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Nova1 antibody (A06087-1). <br>
Overlay histogram showing U87 cells stained with A06087-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nova1 Antibody (A06087-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nox5-picoband-trade-antibody-a03090-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03090-NOX5-primary-antibodies-WB-testing-1.jpgAnti-NOX5 Antibody Picoband® Western blot analysis of NOX5 using anti-NOX5 antibody (A03090).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Hela whole cell lysate,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human SGC-7901 whole cell lysate,<br>Lane 4: human Jurkat whole cell lysate,<br>Lane 5: human THP-1 whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX5 antigen affinity purified polyclonal antibody (Catalog # A03090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX5 at approximately 86KD. The expected band size for NOX5 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03090-nox5-primary-antibodies-if-testing-2.jpgAnti-NOX5 Antibody Picoband® IF analysis of NOX5 using anti-NOX5 antibody (A03090). <br>
NOX5 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NOX5 Antibody (A03090) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03090-nox5-primary-antibodies-fcm-testing-3.jpgAnti-NOX5 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-NOX5 antibody (A03090). <br>
Overlay histogram showing PC-3 cells stained with A03090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX5 Antibody (A03090, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nrf1-antibody-a01129-2-boster.html2026-03-24T05:20:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-WB-testing-1.jpgAnti-NRF1 Antibody Picoband® Western blot analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysate,<br>Lane 2: human MDA-MB-231 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRF1 antigen affinity purified polyclonal antibody (Catalog # A01129-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRF1 at approximately 65KD. The expected band size for NRF1 is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-10.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-2.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-20.pngAnti-NRF1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-NRF1 antibody (A01129-2). <br> Overlay histogram showing A431 cells stained with A01129-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRF1 Antibody (A01129-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-3.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-4.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-5.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-6.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-7.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-8.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01129-2-NRF1-primary-antibodies-IHC-testing-9.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).<br>NRF1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-11.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-12.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-13.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-14.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-15.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-16.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-17.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-18.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01129-2-nrf1-primary-antibodies-ihc-testing-19.jpgAnti-NRF1 Antibody Picoband® IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2). <br> NRF1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NRF1 Antibody (A01129-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-oncostatin-m-picoband-trade-antibody-a00804-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00804-1-oncostatin-m-primary-antibodies-wb-testing-1.jpgAnti-Oncostatin M/Osm Antibody Picoband® Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (A00804-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat lung tissue lysates,<br>Lane 2: rat spleen tissue lysates,<br>Lane 3: mouse lung tissue lysates,<br>Lane 4: mouse spleen tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # A00804-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 28KD. The expected band size for Oncostatin M is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-pafah-picoband-trade-antibody-a02164-2-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02164-2-PAFAH-primary-antibodies-WB-testing-1.jpgAnti-PAFAH/PLA2G7 Antibody Picoband® Western blot analysis of PAFAH using anti-PAFAH antibody (A02164-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human Caco-2 whole cell lysate,<br>Lane 2: human SW620 whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH antigen affinity purified polyclonal antibody (Catalog # A02164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAFAH at approximately 50KD. The expected band size for PAFAH is at 50KD.
https://www.bosterbio.com/products/primary-antibodies/anti-plasminogen-picoband-trade-antibody-a03674-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-1-plg-primary-antibodies-wb-testing-1.jpgAnti-Plasminogen/PLG Antibody Picoband® Western blot analysis of Plasminogen using anti-Plasminogen antibody (A03674-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human Sw620 whole cell lysates, <br>
Lane 5: human Jurkat whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: rat liver tissue lysates, <br>
Lane 8: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasminogen antigen affinity purified polyclonal antibody (Catalog # A03674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plasminogen at approximately 100 kDa. The expected band size for Plasminogen is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-1-fmicb-14-1335658-g008.jpgAnti-Plasminogen/PLG Antibody Picoband®Binding ability of rLP78 to human fibronectin (hFn) and human plasminogen (hPlg). The binding ability of rLP78 to hFn (A) or hPlg (B) was identified by ELISA. The plates were coated with 5 μg/mL of hFn or hPlg. Different concentrations of rLP78 or BSA were added to individual wells. Bound proteins were detected using a anti-His-tag monoclonal antibody. Bars represent the mean ± standard deviation of the OD values of samples in triplicate. The differences were compared between each group. The same letter indicates no obvious difference ( p > 0.05); different letters indicate a significant difference ( p < 0.01). Binding ability of rLP78 to hFn (C) or hPlg (D) was confirmed by western blotting. Bound hFn was determined by rabbit anti-hFn antibody or rabbit anti-plasminogen antibody. BSA was chosen as negative control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10803467/&orig_host=www.ncbi.nlm.nih.gov'>38264482</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-1-plg-primary-antibodies-fcm-testing-2.pngAnti-Plasminogen/PLG Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Plasminogen antibody (A03674-1). <br>Overlay histogram showing U251 cells stained with A03674-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasminogen Antibody (A03674-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pon1-picoband-trade-antibody-a00516-3-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00516-3-pon1-primary-antibodies-wb-testing-1_1.jpgAnti-PON1 Antibody Picoband® Western blot analysis of PON1 using anti-PON1 antibody (A00516-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates,<br>Lane 2: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody (Catalog # A00516-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON1 at approximately 43KD. The expected band size for PON1 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00516-3-PON1-primary-antibodies-IHC-testing-2.jpgAnti-PON1 Antibody Picoband® IHC analysis of PON1 using anti-PON1 antibody (A00516-3).<br>PON1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PON1 Antibody (A00516-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00516-3-PON1-primary-antibodies-IHC-testing-3.jpgAnti-PON1 Antibody Picoband® IHC analysis of PON1 using anti-PON1 antibody (A00516-3).<br>PON1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PON1 Antibody (A00516-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00516-3-PON1-primary-antibodies-IHC-testing-4.jpgAnti-PON1 Antibody Picoband® IHC analysis of PON1 using anti-PON1 antibody (A00516-3).<br>PON1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PON1 Antibody (A00516-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00516-3-PON1-primary-antibodies-IHC-testing-5.jpgAnti-PON1 Antibody Picoband® IHC analysis of PON1 using anti-PON1 antibody (A00516-3).<br>PON1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PON1 Antibody (A00516-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-brn3a-picoband-trade-antibody-a05403-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05403-1-brn3a-primary-antibodies-wb-testing-1.jpgAnti-BRN3A/POU4F1 Antibody Picoband® Western blot analysis of BRN3A using anti-BRN3A antibody (A05403-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human CACO-2 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRN3A antigen affinity purified polyclonal antibody (Catalog # A05403-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRN3A at approximately 45 kDa. The expected band size for BRN3A is at 43 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-ptcra-picoband-trade-antibody-a10240-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10240-1-ptcra-primary-antibodies-wb-testing-1_1.jpgAnti-PTCRA Antibody Picoband® Western blot analysis of PTCRA using anti-PTCRA antibody (A10240-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A375 whole cell lysate,<br>Lane 2: human HL-60 whole cell lysate,<br>Lane 3: human CCRM-CEM whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCRA antigen affinity purified polyclonal antibody (Catalog # A10240-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTCRA at approximately 29KD. The expected band size for PTCRA is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rad51-picoband-trade-antibody-a00088-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00088-rad51-primary-antibodies-wb-testing-1.jpgAnti-Rad51 Antibody Picoband® Western blot analysis of Rad51 using anti-Rad51 antibody (A00088). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate,<br>Lane 2: human A431 whole cell lysate,<br>Lane 3: human 293T whole cell lysate,<br>Lane 4: human K562 whole cell lysate,<br>Lane 5: human Jurkat whole cell lysate,<br>Lane 6: human A549 whole cell lysate,<br>Lane 7: human Caco-2 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # A00088) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 36KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00088-rad51-primary-antibodies-wb-testing-2.jpgAnti-Rad51 Antibody Picoband® Western blot analysis of Rad51 using anti-Rad51 antibody (A00088). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse testis tissue lysates,<br>Lane 3: mouse thymus tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # A00088) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 36KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00088-Rad51-primary-antibodies-IHC-testing-3.jpgAnti-Rad51 Antibody Picoband® IHC analysis of Rad51 using anti-Rad51 antibody (A00088).<br>Rad51 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00088-Rad51-primary-antibodies-IHC-testing-4.jpgAnti-Rad51 Antibody Picoband® IHC analysis of Rad51 using anti-Rad51 antibody (A00088).<br>Rad51 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00088-Rad51-primary-antibodies-IHC-testing-5.jpgAnti-Rad51 Antibody Picoband® IHC analysis of Rad51 using anti-Rad51 antibody (A00088).<br>Rad51 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00088-6.jpgAnti-Rad51 Antibody Picoband® IHC analysis of Rad51 using anti-Rad51 antibody (A00088). <br> Rad51 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00088-rad51-primary-antibodies-if-testing-7.jpgAnti-Rad51 Antibody Picoband® IF analysis of Rad51 using anti-Rad51 antibody (A00088). <br>
Rad51 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Rad51 Antibody (A00088) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00088-rad51-primary-antibodies-fcm-testing-8.jpgAnti-Rad51 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Rad51 antibody (A00088).<br>Overlay histogram showing SiHa cells stained with A00088 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad51 Antibody (A00088,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rasal1-picoband-trade-antibody-a06423-2-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06423-2-rasal1-primary-antibodies-wb-testing-1.jpgAnti-RASAL1/RASAL1 Antibody Picoband® Western blot analysis of RASAL1 using anti-RASAL1 antibody (A06423-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates<br> Lane 2: mouse brain tissue lysates<br> Lane 3: human Raji whole cell lysates <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL1 antigen affinity purified polyclonal antibody (Catalog # A06423-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL1 at approximately 90KD. The expected band size for RASAL1 is at 90KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06423-2-rasal1-primary-antibodies-ihc-testing-7.jpgAnti-RASAL1/RASAL1 Antibody Picoband®IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2). <br>RASAL1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06423-2-rasal1-primary-antibodies-ihc-testing-8.jpgAnti-RASAL1/RASAL1 Antibody Picoband®IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2). <br>RASAL1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06423-2-RASAL1-primary-antibodies-IHC-testing-3.jpgAnti-RASAL1/RASAL1 Antibody Picoband® IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).<br>RASAL1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06423-2-RASAL1-primary-antibodies-IHC-testing-2.jpgAnti-RASAL1/RASAL1 Antibody Picoband® IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).<br>RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06423-2-RASAL1-primary-antibodies-IHC-testing-6.jpgAnti-RASAL1/RASAL1 Antibody Picoband® IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).<br>RASAL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06423-2-RASAL1-primary-antibodies-IHC-testing-5.jpgAnti-RASAL1/RASAL1 Antibody Picoband® IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).<br>RASAL1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06423-2-RASAL1-primary-antibodies-IHC-testing-4.jpgAnti-RASAL1/RASAL1 Antibody Picoband® IHC analysis of RASAL1 using anti-RASAL1 antibody (A06423-2).<br>RASAL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RASAL1 Antibody (A06423-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-rp2-picoband-trade-antibody-a01923-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01923-1-rp2-primary-antibodies-wb-testing-1.jpgAnti-RP2 Antibody Picoband® Western blot analysis of RP2 using anti-RP2 antibody (A01923-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human SGC-7901 whole cell lysate,<br>Lane 3: rat lung tissue lysates,<br>Lane 4: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RP2 antigen affinity purified polyclonal antibody (Catalog # A01923-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01923-1-rp2-primary-antibodies-if-testing-10.jpgAnti-RP2 Antibody Picoband® IF analysis of RP2 using anti-RP2 antibody (A01923-1). <br> RP2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-2.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).<br>RP2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-3.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).<br>RP2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-4.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).<br>RP2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-5.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).<br>RP2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-6.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).<br>RP2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01923-1-RP2-primary-antibodies-IHC-testing-7.jpgAnti-RP2 Antibody Picoband® IHC analysis of RP2 using anti-RP2 antibody (A01923-1).
<br>RP2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RP2 Antibody (A01923-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01923-1-rp2-primary-antibodies-fc-testing-8.pngAnti-RP2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-RP2 antibody (A01923-1). <br> Overlay histogram showing A431 cells stained with A01923-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RP2 Antibody (A01923-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01923-1-rp2-primary-antibodies-fc-testing-9.pngAnti-RP2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-RP2 antibody (A01923-1). <br> Overlay histogram showing THP-1 cells stained with A01923-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RP2 Antibody (A01923-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rps3-picoband-trade-antibody-a01542-2-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-wb-testing-1.jpg_2.jpgAnti-RPS3 Antibody Picoband® Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HL-60 whole cell lysate, <br> Lane 3: human Caco-2 whole cell lysate, <br> Lane 4: human K562 whole cell lysate, <br> Lane 5: human U2OS whole cell lysate, <br> Lane 6: human MCF-7 whole cell lysate, <br> Lane 7: human PC-3 whole cell lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-wb-testing-2.jpg_2.jpgAnti-RPS3 Antibody Picoband® Western blot analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat stomach tissue lysates,<br> Lane 4: mouse brain tissue lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse stomach tissue lysates,<br> Lane 7: mouse kidney tissue lysates, <br> Lane 8: mouse Neuro-2a whole cell lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS3 antigen affinity purified polyclonal antibody (Catalog # A01542-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS3 at approximately 31KD. The expected band size for RPS3 is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-ihc-testing-3.jpg_3.jpgAnti-RPS3 Antibody Picoband® IHC analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> RPS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPS3 Antibody (A01542-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-ihc-testing-4.jpg_3.jpgAnti-RPS3 Antibody Picoband® IHC analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> RPS3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPS3 Antibody (A01542-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-ihc-testing-5.jpg_3.jpgAnti-RPS3 Antibody Picoband® IHC analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> RPS3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPS3 Antibody (A01542-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-ihc-testing-6.jpg_3.jpgAnti-RPS3 Antibody Picoband® IHC analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> RPS3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPS3 Antibody (A01542-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01542-2-rps3-primary-antibodies-ihc-testing-7.jpg_3.jpgAnti-RPS3 Antibody Picoband® IHC analysis of RPS3 using anti-RPS3 antibody (A01542-2). <br> RPS3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPS3 Antibody (A01542-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-rsk3-antibody-a06142-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06142-1-RSK3-primary-antibodies-WB-testing-1.jpgAnti-RSK3/RPS6KA2 Antibody Picoband® Western blot analysis of RSK3 using anti-RSK3 antibody (A06142-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysates,<br>Lane 2: human Hela whole cell lysate,<br>Lane 3: human PC-3 whole cell lysate,<br>Lane 4: human A431 whole cell lysate,<br>Lane 5: human K562 whole cell lysate,<br>Lane 6: human PANC-1 whole cell lysate,<br>Lane 7: rat testis tissue lysates,<br>Lane 8: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody (Catalog # A06142-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.
https://www.bosterbio.com/products/primary-antibodies/anti-gpcr-lgr8-picoband-trade-antibody-a04848-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04848-1-gpcr-lgr8-primary-antibodies-wb-testing-1.jpgAnti-GPCR LGR8/RXFP2 Antibody Picoband® Western blot analysis of GPCR LGR8 using anti-GPCR LGR8 antibody (A04848-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human SHG-44 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR LGR8 antigen affinity purified polyclonal antibody (Catalog # A04848-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR LGR8 at approximately 86KD. The expected band size for GPCR LGR8 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04848-1-GPCR-LGR8-primary-antibodies-FC-testing-2.jpgAnti-GPCR LGR8/RXFP2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-GPCR LGR8 antibody (A04848-1).<br>Overlay histogram showing U251 cells stained with A04848-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR LGR8 Antibody (A04848-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-snap25-picoband-trade-antibody-a01625-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01625-snap25-primary-antibody-wb-testing-1.jpgAnti-SNAP25 Antibody Picoband® Western blot analysis of SNAP25 using anti-SNAP25 antibody (A01625). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (Catalog # A01625) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 25 kDa. The expected band size for SNAP25 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01625-snap25-primary-antibodies-ihc-testing-7.jpgAnti-SNAP25 Antibody Picoband®IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625). <br>SNAP25 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01625-SNAP25-primary-antibodies-IHC-testing-2.jpgAnti-SNAP25 Antibody Picoband® IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).<br>SNAP25 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01625-SNAP25-primary-antibodies-IHC-testing-3.jpgAnti-SNAP25 Antibody Picoband® IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).<br>SNAP25 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01625-SNAP25-primary-antibodies-IHC-testing-4.jpgAnti-SNAP25 Antibody Picoband® IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).<br>SNAP25 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01625-SNAP25-primary-antibodies-IHC-testing-5.jpgAnti-SNAP25 Antibody Picoband® IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625).<br>SNAP25 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01625-snap25-primary-antibody-ihc-testing-6.jpgAnti-SNAP25 Antibody Picoband® IHC analysis of SNAP25 using anti-SNAP25 antibody (A01625). <br>
SNAP25 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP25 Antibody (A01625) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01625-snap25-primary-antibody-if-testing-7.jpgAnti-SNAP25 Antibody Picoband® IF analysis of SNAP25 using anti- SNAP25 antibody (A01625). <br>
SNAP25 was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SNAP25 Antibody (A01625) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01625-snap25-primary-antibodies-fc-testing-8.pngAnti-SNAP25 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SNAP25 antibody (A01625). <br> Overlay histogram showing U20S cells stained with A01625 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP25 Antibody (A01625,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sp1-picoband-trade-antibody-a00110-1-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-fphys-13-843825-g005.jpgAnti-SP1 Antibody Picoband®mRNA and protein levels of TET1 and MYC-TET1 cells were detected. (A) The mRNA expression of SP1 in spermatogonial cells was detected by QRT-PCR. (B) The expression of SP1 in TET1 overexpressed cells was detected by Western Blot. (C) Quantification of SP1 protein levels in TET1 overexpressed cells. (D) The expression of DAXX in TET1 overexpressed cells was detected by Western Blot. (E) Quantification of DAXX protein levels in TET1 overexpressed cells. (F) The expression of P53 in TET1 overexpressed cells was detected by Western Blot. (G) Quantification of P53 protein levels in TET1 overexpressed cells. p < .0001(****), p < .001(***), p < .01(**), p < .05(*).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.843825/full'>35222097</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-fphys-13-843825-g006.jpgAnti-SP1 Antibody Picoband®Immunofluorescence staining of SP1 in overexpressed and control cells cultured in vitro . (A) Immunofluorescence staining of SP1 in overexpressed cells (MYC-TET1) cultured in vitro . (B) Immunofluorescence staining of SP1 in control cells (MYC) cultured in vitro .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.843825/full'>35222097</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-wb-testing-1.jpgAnti-SP1 Antibody Picoband® Western blot analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP1 antigen affinity purified polyclonal antibody (Catalog # A00110-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-2_1.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of human non-samll cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-3_1.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of human tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-4_1.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-5_1.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-6.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-ihc-testing-7.jpgAnti-SP1 Antibody Picoband® IHC analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-if-testing-8.jpgAnti-SP1 Antibody Picoband® IF analysis of SP1 using anti-SP1 antibody (A00110-1) and anti-Beta Tubulin antibody (M01857-3).<br>
SP1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SP1 Antibody (A00110-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-if-testing-9.jpgAnti-SP1 Antibody Picoband® IF analysis of SP1 using anti-SP1 antibody (A00110-1). <br>
SP1 was detected in a paraffin-embedded section of human non-samll cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SP1 Antibody (A00110-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00110-1-sp1-primary-antibodies-fcm-testing-10.jpgAnti-SP1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SP1 antibody (A00110-1). <br>
Overlay histogram showing A431 cells stained with A00110-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SP1 Antibody (A00110-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sparc-picoband-trade-antibody-a00862-2-boster.html2026-03-24T05:20:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00862-2-sparc-primary-antibodies-wb-testing-1.jpgAnti-SPARC Antibody Picoband® Western blot analysis of SPARC using anti-SPARC antibody (A00862-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates,<br>
Lane 2: mouse lung tissue lysates,<br>
Lane 3: mouse small intestines tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-tank-picoband-trade-antibody-a00445-3-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-tank-primary-antibodies-wb-testing-1_1.jpgAnti-TANK Antibody Picoband® Western blot analysis of TANK using anti-TANK antibody (A00445-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human MDA-MB-453 whole cell lysates,<br>
Lane 4: human SW620 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TANK antigen affinity purified polyclonal antibody (Catalog # A00445-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TANK at approximately 48 kDa. The expected band size for TANK is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-tank-primary-antibodies-wb-testing-2.jpgAnti-TANK Antibody Picoband® Western blot analysis of TANK using anti-TANK antibody (A00445-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: rat spleen tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse lung tissue lysates,<br>Lane 7: mouse spleen tissue lysates,<br>Lane 8: mouse kidney tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TANK antigen affinity purified polyclonal antibody (Catalog # A00445-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TANK at approximately 48KD. The expected band size for TANK is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00445-3-TANK-primary-antibodies-IHC-testing-3.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).<br>TANK was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00445-3-TANK-primary-antibodies-IHC-testing-4.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).<br>TANK was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00445-3-TANK-primary-antibodies-IHC-testing-5.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).<br>TANK was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00445-3-TANK-primary-antibodies-IHC-testing-6.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).<br>TANK was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00445-3-TANK-primary-antibodies-IHC-testing-7.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).
<br>TANK was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-tank-primary-antibodies-fc-testing-8.jpg.pngAnti-TANK Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-TANK antibody (A00445-3). <br> Overlay histogram showing A431 cells stained with A00445-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TANK Antibody (A00445-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-9.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3). <br> TANK was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-10.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3). <br> TANK was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00445-3-11_1.jpgAnti-TANK Antibody Picoband® IHC analysis of TANK using anti-TANK antibody (A00445-3).<br> TANK was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TANK Antibody (A00445-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-tnf-alpha-antibody-a00002-3-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00002-3-tnf-alpha-primary-antibodies-wb-testing-1.jpgAnti-TNF alpha Antibody Picoband® Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br>
Lane 1: recombinant human TNF alpha protein 10 ng,<br>
Lane 2: recombinant human TNF alpha protein 5 ng,<br>
Lane 3: recombinant human TNF alpha protein 1 ng,<br>
Lane 4: recombinant human TNF alpha protein 0.5 ng.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (A00002-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00002-3-tnf-alpha-primary-antibodies-ihc-testing-1.jpgAnti-TNF alpha Antibody Picoband®IHC analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). <br>TNF alpha was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNF alpha Antibody (A00002-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00002-3-tnf-alpha-primary-antibodies-ihc-testing-2.jpgAnti-TNF alpha Antibody Picoband®IHC analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). <br>TNF alpha was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNF alpha Antibody (A00002-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-tnf-receptor-i-picoband-trade-antibody-a00294-3-boster.html2026-04-01T05:01:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00294-3-tnfrsf1a-primary-antibodies-wb-testing-1.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband® Western blot analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294-3). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human A549 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1A antigen affinity purified polyclonal antibody (A00294-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 55 kDa. The expected band size for TNFRSF1A is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00294-3-tnfrsf1a-primary-antibodies-wb-testing-2.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband® Western blot analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294-3). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: rat lung tissue lysates,<br>
Lane 3: mouse brain tissue lysates,<br>
Lane 4: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1A antigen affinity purified polyclonal antibody (A00294-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 55 kDa. The expected band size for TNFRSF1A is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00294-3-tnfrsf1a-primary-antibodies-ihc-testing-3.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband® IHC analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294-3). <br>TNFRSF1A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFRSF1A Antibody (A00294-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00294-3-tnfrsf1a-primary-antibodies-ihc-testing-4.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband® IHC analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294-3). <br>TNFRSF1A was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFRSF1A Antibody (A00294-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00294-3-tnfrsf1a-primary-antibodies-ihc-testing-5.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband®IHC analysis of TNFR1/TNFRSF1A using anti-TNFR1/TNFRSF1A antibody (A00294-3). <br>TNFR1/TNFRSF1A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFR1/TNFRSF1A Antibody (A00294-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd137-picoband-trade-antibody-a00867-2-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00867-2-cd137-primary-antibodies-wb-testing-1.jpgAnti-CD137/Tnfrsf9 Antibody Picoband® Western blot analysis of CD137 using anti-CD137 antibody (A00867-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br>Lane 1: recombinant mouse CD137 protein 1ng. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD137 antigen affinity purified polyclonal antibody (Catalog # A00867-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD137 at approximately 65KD. The expected band size for CD137 is at 44KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00867-2-cd137-primary-antibodies-elisa-testing-2.jpgAnti-CD137/Tnfrsf9 Antibody Picoband® Sandwich ELISA - Recombinant mouse CD137/Tnfrsf9 protein standard curve.<br>
Use in combination with reagents from Mouse CD137/Tnfrsf9 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1143).
https://www.bosterbio.com/products/primary-antibodies/anti-cardiac-troponin-t-picoband-trade-antibody-a01154-1-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01154-1-Cardiac-Troponin-T-primary-antibodies-WB-testing-1.jpgAnti-Cardiac Troponin T/TNNT2 Antibody Picoband® Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat heart tissue lysates,<br>Lane 2: rat heart tissue lysates,<br>Lane 3: mouse heart tissue lysates,<br>Lane 4: mouse heart tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
https://www.bosterbio.com/products/primary-antibodies/anti-tcr-alpha-picoband-trade-antibody-a05315-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-wb-testing-1.jpgAnti-TCR alpha/TRAC Antibody Picoband® Western blot analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCR alpha/TRAC antigen affinity purified polyclonal antibody (Catalog # A05315) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCR alpha/TRAC at approximately 45 kDa. The expected band size for TCR alpha/TRAC is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-ihc-testing-2.jpgAnti-TCR alpha/TRAC Antibody Picoband® IHC analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
TCR alpha/TRAC was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCR alpha/TRAC Antibody (A05315) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-ihc-testing-3.jpgAnti-TCR alpha/TRAC Antibody Picoband® IHC analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
TCR alpha/TRAC was detected in a paraffin-embedded section of human urothelium carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCR alpha/TRAC Antibody (A05315) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-ihc-testing-4.jpgAnti-TCR alpha/TRAC Antibody Picoband® IHC analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
TCR alpha/TRAC was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCR alpha/TRAC Antibody (A05315) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-ihc-testing-5.jpgAnti-TCR alpha/TRAC Antibody Picoband® IHC analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
TCR alpha/TRAC was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCR alpha/TRAC Antibody (A05315) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-ihc-testing-6.jpgAnti-TCR alpha/TRAC Antibody Picoband® IHC analysis of TCR alpha/TRAC using anti-TCR alpha/TRAC antibody (A05315). <br>
TCR alpha/TRAC was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCR alpha/TRAC Antibody (A05315) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05315-trac-primary-antibodies-fcm-testing-7.jpgAnti-TCR alpha/TRAC Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-TCR alpha/TRAC antibody (A05315). <br>
Overlay histogram showing Jurkat cells stained with A05315 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TCR alpha/TRAC Antibody (A05315, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-trpm7-picoband-trade-antibody-a00789-1-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00789-1-trpm7-primary-antibodies-wb-testing-1.jpgAnti-TRPM7 Antibody Picoband® Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysate,<br>Lane 2: human COLO-320 whole cell lysate,<br>Lane 3: human 22RV1 whole cell lysate,<br>Lane 4: human SGC-7901 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00789-1-trpm7-primary-antibodies-if-testing-2.jpgAnti-TRPM7 Antibody Picoband® IF analysis of TRPM7 using anti-TRPM7 antibody (A00789-1). <br>
TRPM7 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRPM7 Antibody (A00789-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00789-1-trpm7-primary-antibodies-fcm-testing-3.jpgAnti-TRPM7 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TRPM7 antibody (A00789-1).<br>Overlay histogram showing THP-1 cells stained with A00789-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPM7 Antibody (A00789-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-thioredoxin-trx-picoband-trade-antibody-a01219-1-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01219-1-txn-primary-antibodies-wb-testing-1.jpgAnti-Thioredoxin/TRX/Txn Antibody Picoband® Western blot analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat kidney tissue lysates,<br>
Lane 2: rat small intestine tissue lysates,<br>
Lane 3: rat spleen tissue lysates,<br>
Lane 4: rat lung tissue lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse kidney tissue lysates,<br>
Lane 7: mouse small intestine tissue lysates,<br>
Lane 8: mouse lung tissue lysates,<br>
Lane 9: mouse heart tissue lysates,<br>
Lane 10: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody (Catalog # A01219-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thioredoxin TRX at approximately 12 kDa. The expected band size for Thioredoxin TRX is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01219-1-thioredoxin-trx-primary-antibodies-ihc-testing-2.jpgAnti-Thioredoxin/TRX/Txn Antibody Picoband® IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).<br>Thioredoxin TRX was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01219-1-thioredoxin-trx-primary-antibodies-ihc-testing-3.jpgAnti-Thioredoxin/TRX/Txn Antibody Picoband® IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).<br>Thioredoxin TRX was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01219-1-thioredoxin-trx-primary-antibodies-ihc-testing-4.jpgAnti-Thioredoxin/TRX/Txn Antibody Picoband® IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).<br> Thioredoxin TRX was detected in immunocytochemical section of LLC cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01219-1-thioredoxin-trx-primary-antibodies-if-testing-5.jpgAnti-Thioredoxin/TRX/Txn Antibody Picoband® IF analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1). <br> Thioredoxin TRX was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Thioredoxin TRX Antibody (A01219-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ube2i-ubc9-picoband-trade-antibody-a02295-boster.html2026-03-24T05:20:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-primary-antibodies-wb-testing-1.jpgAnti-UBE2I UBC9 Antibody Picoband® Western blot analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: rat kidney tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2I UBC9 antigen affinity purified polyclonal antibody (Catalog # A02295) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2I UBC9 at approximately 18 kDa. The expected band size for UBE2I UBC9 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-2.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-3.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-4.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-5.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-6.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-7.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-8.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02295-UBE2I-UBC9-primary-antibodies-IHC-testing-9.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody (A02295).<br>UBE2I UBC9 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-10.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-11.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-12.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-13.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-14.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-ihc-testing-15.jpgAnti-UBE2I UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ube2i-ubc9-primary-antibodies-if-testing-16.jpg.jpgAnti-UBE2I UBC9 Antibody Picoband® IF analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295). <br> UBE2I/UBC9 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UBE2I/UBC9 Antibody (A02295) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-ubc9-primary-antibodies-fcm-testing-17.jpgAnti-UBE2I UBC9 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-UBE2I/UBC9 antibody (A02295). <br>
Overlay histogram showing A431 cells stained with A02295 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2I/UBC9 Antibody (A02295, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-tnfrsf17-bcma-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0661-boster.html2026-03-24T05:20:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0661.pngHuman TNFRSF17/BCMA ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human TNFRSF17/BCMA EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/human-cxcl10-ip-10-elisa-kit-ez-set-trade-diy-antibody-pairs.html2026-03-24T05:20:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0735-human-cxcl10-ez-set-elisa-kit-diy-antibody-pairs.jpgHuman CXCL10/IP-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human CXCL10/IP-10 EZ-Set ELISA Kit standard curve
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https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-klk1-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0903-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0903-human-klk1-ez-set-elisa-kit-diy-antibody-pairs.jpgHuman KLK1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human KLK1 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/mouse-vegf-b-elisa-kit-ez-set-trade-diy-antibody-pairs.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1411_1.pngMouse VEGF-B ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse VEGF-B EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-b2m-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1691-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1691.pngHuman B2M ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human B2M EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-transthyretin-ttr-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1684-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1684.pngHuman Transthyretin/TTR ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human TTR EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ada-picoband-trade-antibody-monoclonal-m00866-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-wb-testing-1_1.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) Western blot analysis of ADA using anti-ADA antibody (M00866). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human placenta tissue lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: human U-937 whole cell lysates,<br> Lane 6: human U20S whole cell lysates,<br> Lane 7: human Caco-2 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody (Catalog # M00866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-ihc-testing-2.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) IHC analysis of ADA using anti-ADA antibody (M00866). <br> ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADA Antibody (M00866) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-ihc-testing-3.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) IHC analysis of ADA using anti-ADA antibody (M00866). <br> ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADA Antibody (M00866) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-ihc-testing-4.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) IHC analysis of ADA using anti-ADA antibody (M00866). <br> ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADA Antibody (M00866) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-ihc-testing-5.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) IHC analysis of ADA using anti-ADA antibody (M00866). <br> ADA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADA Antibody (M00866) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-ihc-testing-6.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) IHC analysis of ADA using anti-ADA antibody (M00866). <br> ADA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADA Antibody (M00866) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-ada-primary-antibodies-fc-testing-7.jpgAnti-ADA Antibody Picoband® (monoclonal, 6D4) Flow Cytometry analysis of U20S cells using anti-ADA antibody (M00866).<br> Overlay histogram showing U20S cells stained with M00866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ADA Antibody (M00866,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmt55-p54nrb-picoband-trade-antibody-monoclonal-m03515-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03515-nmt55-p54nrb-primary-antibodies-wb-testing-1_1_1.jpgAnti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) Western blot analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody (M03515). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human SW620 whole cell lysates, <br>
Lane 6: human PANC-1 whole cell lysates, <br>
Lane 7: human U20S whole cell lysates, <br>
Lane 8: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-nmt55 p54nrb antigen affinity purified monoclonal antibody (Catalog # M03515) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for nmt55 p54nrb at approximately 60KD. The expected band size for nmt55 p54nrb is at 60KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03515-nmt55-p55nrb-primary-antibodies-fc-testing-2.jpgAnti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) Flow Cytometry analysis of U20S cells using anti-nmt55 p54nrb antibody (M03515). <br> Overlay histogram showing U20S cells stained with M03515 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03515-nmt55-p56nrb-primary-antibodies-ihc-testing-3.jpgAnti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) IHC analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody (M03515).<br> nmt55 p54nrb was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-nmt55 p54nrb Antibody (M03515) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03515-nmt55-p54nrb-primary-antibodies-if-testing-4.jpgAnti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) IF analysis of nmt55 p54nrb using anti-nmt55 p54nrb antibody (M03515). <br>
nmt55 p54nrb was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-nmt55 p54nrb Antibody (M03515) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-actn3-picoband-trade-antibody-monoclonal-m02693-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02693-actn3-primary-antibodies-wb-testing-1_1.jpgAnti-ACTN3 Antibody Picoband® (monoclonal, 9B5) Western blot analysis of ACTN3 using anti-ACTN3 antibody (M02693). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue,<br> Lane 2: mouse skeletal muscle tissue. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ACTN3 antigen affinity purified monoclonal antibody (Catalog # M02693) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02693-actn3-primary-antibodies-ihc-testing-2.jpgAnti-ACTN3 Antibody Picoband® (monoclonal, 9B5) IHC analysis of ACTN3 using anti-ACTN3 antibody (M02693). <br> ACTN3 was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ACTN3 Antibody (M02693) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il22-picoband-trade-antibody-monoclonal-m00963-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00963-il22-primary-antibodies-fc-testing-1.jpgAnti-IL22 Antibody Picoband® (monoclonal, 7F2) Flow Cytometry analysis of HL-60 cells using anti-IL22 antibody (M00963).<br> Overlay histogram showing HL-60 cells stained with M00963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IL22 Antibody (M00963,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00963-2.jpgAnti-IL22 Antibody Picoband® (monoclonal, 7F2) Western blot analysis of IL22 using anti-IL22 antibody (M00963). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysates, <br> Lane 2: human A375 whole cell lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IL22 antigen affinity purified monoclonal antibody (Catalog # M00963) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24KD. The expected band size for IL22 is at 20KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd2ap-picoband-trade-antibody-monoclonal-m01756-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-wb-testing-1_3.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8)Western blot analysis of CD2AP using anti-CD2AP antibody (M01756).
<br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates.<br>
Lane 4: human 22RV1 whole cell lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse HEPA1/6 whole cell lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After Electrophoresis, proteins were tCD2APsferred to a Nitrocellulose membCD2APe at 150mA for 50-90 minutes. Blocked the membCD2APe with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membCD2APe was incubated with mouse anti-CD2AP antigen affinity purified monoclonal antibody (Catalog # M01756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD2AP at approximately 80 kDa. The expected band size for CD2AP is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-2.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756). <br> CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-3.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756). <br> CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epi1ope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-4.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756). <br> CD2AP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-5.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756). <br> CD2AP was detected in paraffin-embedded section of human mammary cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-7.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of human mammary cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-8.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-9.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-10.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-11.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-12_1.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-13.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) IHC analysis of CD2AP using anti-CD2AP antibody (M01756).<br> CD2AP was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-14.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) ICC analysis of CD2AP using anti-CD2AP antibody (M01756). <br> CD2AP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-CD2AP Antibody (M01756) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-ihc-testing-15.pngAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) Flow Cytometry analysis of U20S cells using anti-CD2AP antibody (M01756).<br> Overlay histogram showing U20S cells stained with M01756 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD2AP Antibody (M01756,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01756-cd2ap-primary-antibodies-fc-testing-6.jpgAnti-CD2AP Antibody Picoband® (monoclonal, 5F8) Flow Cytometry analysis of K562 cells using anti-D2AP antibody (M01756).<br> Overlay histogram showing K562 cells stained with M01756 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-D2AP Antibody (M01756,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt2-picoband-trade-antibody-monoclonal-m00725-1-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00725-1-akt2-primary-antibodies-wb-testing-1.jpgAnti-AKT2 Antibody Picoband® (monoclonal, 10C6) Western blot analysis of AKT2 using anti-AKT2 antibody (M00725-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysate,<br> Lane 2: human 293T whole cell lysate,<br> Lane 3: human HELA whole cell lysate,<br> Lane 4: human Caco-2 whole cell lysate,<br> Lane 5: human K562 whole cell lysate,<br> Lane 6: human HL-60 whole cell lysate,<br> Lane 7: human PC-3 whole cell lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKT2 antigen affinity purified monoclonal antibody (Catalog # M00725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00725-1-akt2-primary-antibodies-if-testing-2.jpgAnti-AKT2 Antibody Picoband® (monoclonal, 10C6) IF analysis of AKT2 using anti-AKT2 antibody (M00725-1). <br>
AKT2 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-AKT2 Antibody (M00725-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00725-1-akt2-primary-antibodies-fcm-testing-3.jpgAnti-AKT2 Antibody Picoband® (monoclonal, 10C6) Flow Cytometry analysis of A549 cells using anti-AKT2 antibody (M00725-1).<br>Overlay histogram showing A549 cells stained with M00725-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AKT2 Antibody (M00725-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-angiostatin-k1-3-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0905-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0905.jpgHuman Angiostatin K1-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Angiostatin K1-3 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-dermatopontin-dpt-picokine-trade-elisa-kit-ek1633-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1633.jpgHuman Dermatopontin/DPT ELISA Kit PicoKine®Human Dermatopontin/DPT PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-alpha-defensin-1-defa1-picokine-trade-elisa-kit-ek1514-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1514.jpgHuman alpha-defensin 1/DEFA1 ELISA Kit PicoKine®Human alpha-defensin/DEFA1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-fgf2-picokine-trade-elisa-kit-ek0342-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0342_1.pngHuman FGF2 ELISA Kit PicoKine®Human FGF2 ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-transthyretin-ttr-picokine-trade-elisa-kit-ek1684-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1684.jpgHuman Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®Human Transthyretin/TTR PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-b2m-picokine-trade-elisa-kit-ek1691-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1691.pngHuman B2M / beta 2 Microglobulin ELISA Kit PicoKine®Human B2M PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-a1-aat-picokine-trade-elisa-kit-ek1634-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1634.jpgHuman Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®Human Serpin A1/alpha 1-Antitrypsin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-trkc-picokine-trade-elisa-kit-ek1676-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1676.pngHuman TRKC ELISA Kit PicoKine®Human TRKC PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-trem2-picokine-trade-elisa-kit-ek1678-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1678.pngHuman TREM2 ELISA Kit PicoKine®Human TREM2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-h-picokine-trade-elisa-kit-ek0674-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0674.pngHuman Cathepsin H ELISA Kit PicoKine®Human Cathepsin H PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-klotho-picokine-trade-elisa-kit-ek1688-boster.html2026-03-24T05:20:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1688.pngHuman Klotho ELISA Kit PicoKine®Human Klotho PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-uromodulin-umod-picokine-trade-elisa-kit-ek1690-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1690.jpgHuman Uromodulin/umod ELISA Kit PicoKine®Human Umod PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/human-defa1-elisa-kit-ez-set-trade-diy-antibody-pairs.html2026-03-24T05:20:45+00:00daily0.9
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-1-beta-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0392-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0392_2.pngHuman IL-1 beta ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-1 beta EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-mif-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0813-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0813.jpgHuman MIF ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human MIF EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-plat-tpa-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0897-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0897.jpgHuman PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human PLAT/TPA EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-madcam-1-picokine-trade-elisa-kit-ek1687-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1687.jpgHuman MADCAM-1 ELISA Kit PicoKine®Human MADCAM-1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-haptoglobin-picokine-trade-elisa-kit-ek1689-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1689.jpgMouse Haptoglobin ELISA Kit PicoKine®Mouse Haptoglobin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-ulbp1-picokine-trade-elisa-kit-ek1685-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1685.jpgHuman ULBP1 ELISA Kit PicoKine®Human ULBP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-pro-collagen-i-alpha-1-col1a1-picokine-trade-elisa-kit-ek1692-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1692_1.pngHuman Pro-collagen Iα1/COL1A1 ELISA Kit PicoKine®Human COL1A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-scgb3a1-picokine-trade-elisa-kit-ek1694-boster.html2026-03-24T05:20:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1694.pngMouse SCGB3A1 ELISA Kit PicoKine®Mouse SCGB3A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-fgfbp1-picokine-trade-elisa-kit-ek1706-boster.html2026-03-24T05:20:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1706.jpgHuman FGFBP1 ELISA Kit PicoKine®Human FGFBP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-fgfbp1-picokine-trade-elisa-kit-ek1695-boster.html2026-03-24T05:20:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1695.jpgRat FGFBP1 ELISA Kit PicoKine®Rat FGFBP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-agrin-picokine-trade-elisa-kit-ek1699-boster.html2026-03-24T05:20:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1699.pngRat Agrin ELISA Kit PicoKine®Rat Agrin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-pdgf-aa-picokine-trade-elisa-kit-ek1696-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1696.pngHuman PDGF-AA ELISA Kit PicoKine®Human PDGF-AA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-pdgf-bb-picokine-trade-elisa-kit-ek1697-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1697.pngMouse PDGF-BB ELISA Kit PicoKine®Mouse PDGF-BB PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-pdgf-bb-picokine-trade-elisa-kit-ek1698-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1698.pngRat PDGF-BB ELISA Kit PicoKine®Rat PDGF-BB PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-pdgf-dd-picokine-trade-elisa-kit-ek1700-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1700.pngHuman PDGF-DD ELISA Kit PicoKine®Human PDGF-DD PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-pcsk9-picokine-trade-elisa-kit-ek1701-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1701.pngRat PCSK9 ELISA Kit PicoKine®Rat PCSK9 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-haptoglobin-picokine-trade-elisa-kit-ek1702-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1702.jpgHuman Haptoglobin ELISA Kit PicoKine®Human Haptoglobin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-gpc2-picokine-trade-elisa-kit-ek1715-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1715.pngMouse GPC2 ELISA Kit PicoKine®Mouse GPC2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-serpinf2-picokine-trade-elisa-kit-ek1713-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1713.jpgMouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®Mouse SerpinF2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-aat-picokine-trade-elisa-kit-ek1711-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1711.jpgMouse Serpin A1 ELISA Kit PicoKine®Mouse AAT PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-hrg-picokine-trade-elisa-kit-ek1708-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1708_1.pngMouse HRG ELISA Kit PicoKine®Mouse HRG PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpinf2-picokine-trade-elisa-kit-ek1712-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1712.jpgHuman Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®Human SerpinF2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-hrg-picokine-trade-elisa-kit-ek1707-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1707.pngHuman HRG ELISA Kit PicoKine®Human HRG PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-2-microglobulin-picoband-trade-antibody-monoclonal-m00456-2-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta_2_microglobulin-primary-antibodies-wb-testing-1.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) Western blot analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: COS-7 whole cell lysates, <br>
Lane 2: HELA whole cell lysates, <br>
Lane 3: HL-60 whole cell lysates, <br>
Lane 4: HEPG2 whole cell lysates, <br>
Lane 5: K562 whole cell lysates, <br>
Lane 6: 293T whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Beta 2 Microglobulin antigen affinity purified monoclonal antibody (Catalog # M00456-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Beta 2 Microglobulin at approximately 12KD. The expected band size for Beta 2 Microglobulin is at 12KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta_2_microglobulin-primary-antibodies-ihc-testing-2.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2). <br>
Beta 2 Microglobulin was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Beta 2 Microglobulin Antibody (M00456-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta_2_microglobulin-primary-antibodies-ihc-testing-3.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2). <br>
Beta 2 Microglobulin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Beta 2 Microglobulin Antibody (M00456-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta_2_microglobulin-primary-antibodies-ihc-testing-4.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) IHC analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2). <br>
Beta 2 Microglobulin was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Beta 2 Microglobulin Antibody (M00456-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta-2-microglobulin-primary-antibodies-if-testing-6.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10) IF analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2). <br>
Beta 2 Microglobulin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Beta 2 Microglobulin Antibody (M00456-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00456-2-beta_2_microglobulin-primary-antibodies-fcm-testing-5.jpgAnti-Beta 2 Microglobulin B2M Antibody Picoband® (monoclonal, 2H10)8. Flow Cytometry analysis of A431 cells using anti-Beta 2 Microglobulin antibody (M00456-2).<br>Overlay histogram showing A431 cells stained with M00456-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beta 2 Microglobulin Antibody (M00456-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppid-picoband-trade-antibody-monoclonal-m02424-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-wb-testing-1.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) Western blot analysis of PPID using anti-PPID antibody (M02424). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: HL-60 whole cell lysates, <br>
Lane 2: K562 whole cell lysates, <br>
Lane 3: THP-1 whole cell lysates, <br>
Lane 4: COS-7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PPID antigen affinity purified monoclonal antibody (Catalog # M02424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PPID at approximately 41KD. The expected band size for PPID is at 41KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-wb-testing-2.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) Western blot analysis of PPID using anti-PPID antibody (M02424). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse liver tissue lysates, <br>
Lane 6: HEPA1-6 whole cell lysate. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PPID antigen affinity purified monoclonal antibody (Catalog # M02424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PPID at approximately 41KD. The expected band size for PPID is at 41KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-ihc-testing-3.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IHC analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPID Antibody (M02424) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-ihc-testing-4.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IHC analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPID Antibody (M02424) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-ihc-testing-5.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IHC analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPID Antibody (M02424) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-ihc-testing-6.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IHC analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPID Antibody (M02424) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-ihc-testing-7.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IHC analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPID Antibody (M02424) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-fcm-testing-8.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) Flow Cytometry analysis of A431 cells using anti-PPID antibody (M02424).<br>Overlay histogram showing A431 cells stained with M02424 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPID Antibody (M02424,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-ppid-primary-antibodies-if-testing-9.jpgAnti-PPID Antibody Picoband® (monoclonal, 5A6) IF analysis of PPID using anti-PPID antibody (M02424). <br>
PPID was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-PPID Antibody (M02424) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ewsr1-picoband-trade-antibody-monoclonal-m00589-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00589-ewsr1-primary-antibodies-wb-testing-1.jpgAnti-EWSR1 Antibody Picoband® (monoclonal, 4B4) Western blot analysis of EWSR1 using anti-EWSR1 antibody (M00589). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: K562 whole cell lysates, <br>
Lane 2: Jurkat whole cell lysates, <br>
Lane 3: A549 whole cell lysates, <br>
Lane 4: MCF-7 whole cell lysates, <br>
Lane 5: COLO-320 whole cell lysates, <br>
Lane 6: SW620 whole cell lysates, <br>
Lane 7: A431 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EWSR1 antigen affinity purified monoclonal antibody (Catalog # M00589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95KD. The expected band size for EWSR1 is at 95KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00589-ewsr1-primary-antibodies-ihc-testing-2.jpgAnti-EWSR1 Antibody Picoband® (monoclonal, 4B4) IHC analysis of EWSR1 using anti-EWSR1 antibody (M00589). <br>
EWSR1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00589-ewsr1-primary-antibodies-ihc-testing-3.jpgAnti-EWSR1 Antibody Picoband® (monoclonal, 4B4) IHC analysis of EWSR1 using anti-EWSR1 antibody (M00589). <br>
EWSR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calpain-2-picoband-trade-antibody-monoclonal-m03492-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-wb-testing-1.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) Western blot analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: A549 whole cell lysates, <br>
Lane 2: U20S whole cell lysates, <br>
Lane 3: COS-7 whole cell lysates, <br>
Lane 4: K562 whole cell lysates, <br>
Lane 5: HELA whole cell lysates, <br>
Lane 6: HEPG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calpain 2 antigen affinity purified monoclonal antibody (Catalog # M03492) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calpain 2 at approximately 80KD. The expected band size for Calpain 2 is at 80KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-ihc-testing-2.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) IHC analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Calpain 2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Calpain 2 Antibody (M03492) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-ihc-testing-3.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) IHC analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Calpain 2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Calpain 2 Antibody (M03492) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-ihc-testing-4.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) IHC analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Calpain 2 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Calpain 2 Antibody (M03492) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-if-testing-5.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) IF analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Calpain 2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Calpain 2 Antibody (M03492) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-if-testing-6.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6) IF analysis of Calpain 2 using anti-Calpain 2 antibody (M03492). <br>
Calpain 2 was detected in immunocytochemical section of U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Calpain 2 Antibody (M03492) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03492-calpain_2-primary-antibodies-fcm-testing-7.jpgAnti-Calpain 2 CAPN2 Antibody Picoband® (monoclonal, 8I6)7. Flow Cytometry analysis of A431 cells using anti-Calpain 2 antibody (M03492).<br>Overlay histogram showing A431 cells stained with M03492 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calpain 2 Antibody (M03492,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rpa70-picoband-trade-antibody-monoclonal-m01317-2-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-wb-testing-1.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) Western blot analysis of RPA70 using anti-RPA70 antibody (M01317-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: HELA whole cell lysates, <br>
Lane 2: MCF-7 whole cell lysates, <br>
Lane 3: K562 whole cell lysates, <br>
Lane 4: COS-7 whole cell lysates, <br>
Lane 5: Caco-2 whole cell lysates, <br>
Lane 6: A549 whole cell lysates, <br>
Lane 7: HEPG2 whole cell lysates, <br>
Lane 8: PC-3 whole cell lysates, <br>
Lane 9: HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RPA70 antigen affinity purified monoclonal antibody (Catalog # M01317-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RPA70 at approximately 12KD. The expected band size for RPA70 is at 12KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-ihc-testing-2.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) IHC analysis of RPA70 using anti-RPA70 antibody (M01317-2). <br>
RPA70 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RPA70 Antibody (M01317-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-ihc-testing-3.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) IHC analysis of RPA70 using anti-RPA70 antibody (M01317-2). <br>
RPA70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RPA70 Antibody (M01317-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-ihc-testing-4.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) IHC analysis of RPA70 using anti-RPA70 antibody (M01317-2). <br>
RPA70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RPA70 Antibody (M01317-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-if-testing-5.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4) IF analysis of RPA70 using anti-RPA70 antibody (M01317-2). <br>
RPA70 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RPA70 Antibody (M01317-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01317-2-rpa70-primary-antibodies-fcm-testing-6.jpgAnti-RPA70 RPA1 Antibody Picoband® (monoclonal, 11H4)6. Flow Cytometry analysis of A431 cells using anti-RPA70 antibody (M01317-2).<br>Overlay histogram showing A431 cells stained with M01317-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RPA70 Antibody (M01317-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fh-picoband-trade-antibody-monoclonal-m02097-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02097-fh-primary-antibodies-wb-testing-1_1.jpgAnti-FH Antibody Picoband® (monoclonal, 9D8) Western blot analysis of FH using anti-FH antibody (M02097). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: K562 whole cell lysates, <br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: COS-7 whole cell lysates, <br>
Lane 4: HL-60 whole cell lysates, <br>
Lane 5: Caco-2 whole cell lysates, <br>
Lane 6: U20S whole cell lysates, <br>
Lane 7: A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02097-fh-primary-antibodies-wb-testing-2_1.jpgAnti-FH Antibody Picoband® (monoclonal, 9D8) Western blot analysis of FH using anti-FH antibody (M02097). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: rat testicular tissue lysates, <br>
Lane 3: rat stomach tissue lysates, <br>
Lane 4: mouse testicular tissue lysates, <br>
Lane 5: mouse kidney tissue lysates, <br>
Lane 6: NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02097-fh-primary-antibodies-ihc-testing-3_1.jpgAnti-FH Antibody Picoband® (monoclonal, 9D8) IHC analysis of FH using anti-FH antibody (M02097). <br>
FH was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-FH Antibody (M02097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02097-fh-primary-antibodies-ihc-testing-4_1.jpgAnti-FH Antibody Picoband® (monoclonal, 9D8) IHC analysis of FH using anti-FH antibody (M02097). <br>
FH was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-FH Antibody (M02097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02097-fh-primary-antibodies-ihc-testing-5.jpgAnti-FH Antibody Picoband® (monoclonal, 9D8) IHC analysis of FH using anti-FH antibody (M02097). <br>
FH was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-FH Antibody (M02097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/picokine-elisa-kits/rat-wisp1-picokine-trade-elisa-kit-ek1588-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1588.pngRat WISP1/CCN4 ELISA Kit PicoKine®Rat WISP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-galectin-1-picokine-trade-elisa-kit-ek1589-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1589.pngRat Galectin-1 ELISA Kit PicoKine®Rat Galectin-1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-efemp1-picokine-trade-elisa-kit-ek1590-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1590.jpgMouse EFEMP1 ELISA Kit PicoKine®Mouse EFEMP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-fgf23-picokine-trade-elisa-kit-ek1591-boster.html2026-03-24T05:20:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1591_2.pngRat FGF23 ELISA Kit PicoKine®Rat FGF23 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-p-cadherin-picokine-trade-elisa-kit-ek1594-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1594.pngRat P-Cadherin-3 CDH3 ELISA Kit PicoKine®Rat P-Cadherin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-fgf21-picokine-trade-elisa-kit-ek1595-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1595_4.pngRat FGF21 ELISA Kit PicoKine®Rat FGF21 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-trkb-picokine-trade-elisa-kit-ek1596-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1596.jpgRat TRKB ELISA Kit PicoKine®Rat TRKB PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-gsta-picokine-trade-elisa-kit-ek1625-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1625.pngHuman GSTA ELISA Kit PicoKine®Human GSTA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/rat-angiopoietin-1-elisa-kit-ez-set-trade-diy-antibody-pairs.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1295-2-elisa-rat-angiopoietin-1-picokine-elisa-kit.jpgRat Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Angiopoietin-1 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-angiopoietin-1-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0559-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0559-2-elisa-human-angiopoietin-1-picokine-elisa-kit.jpgHuman Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Angiopoietin-1 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-angiopoietin-1-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1296-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1296.pngMouse Angiopoietin-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse Angiopoietin-1 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/anti-anillin-anln-picoband-trade-antibody-a03997-1-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03997-1-anln-primary-antibodies-wb-testing-1_1.jpgAnti-Anillin/ANLN Antibody Picoband® Western blot analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: rat PC-12 whole cell lysates, <br>
Lane 4: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Anillin/ANLN antigen affinity purified polyclonal antibody (A03997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Anillin/ANLN at approximately 150 kDa. The expected band size for Anillin/ANLN is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03997-1-anln-primary-antibodies-if-testing-2_1.jpgAnti-Anillin/ANLN Antibody Picoband® IF analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1) and anti-Tubulin Alpha antibody (M03989-3).<br>
Anillin/ANLN was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Anillin/ANLN Antibody (A03997-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Catalog # BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (Catalog # BA1032) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03997-1-anln-primary-antibodies-ip-testing-3.jpgAnti-Anillin/ANLN Antibody Picoband® Immunoprecipitating Anillin/ANLN in U251 whole cell lysate.<br>
Western blot analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1); <br>
Lane 1: U251 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-Anillin/ANLN antibody in U251 whole cell lysate;<br>
Lane 3: anti-Anillin/ANLN antibody (2μg) + U251 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Anillin/ANLN antigen affinity purified polyclonal antibody (A03997-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Anillin/ANLN at approximately 150 kDa. The expected band size for Anillin/ANLN is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03997-1-anln-primary-antibodies-fcm-testing-4.jpgAnti-Anillin/ANLN Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Anillin/ANLN antibody (A03997-1). <br>
Overlay histogram showing U251 cells stained with A03997-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Anillin/ANLN Antibody (A03997-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-bcl6-picoband-trade-antibody-a00142-1-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00142-1-bcl6-primary-antibodies-wb-testing-1.jpgAnti-BCL6 Antibody Picoband®Western blot analysis of BCL6 using anti-BCL6 antibody (A00142-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Ramos whole cell lysates,<br>
Lane 2: human Daudi whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL6 antigen affinity purified polyclonal antibody (A00142-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL6 at approximately 90-95 kDa. The expected band size for BCL6 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00142-1-bcl6-primary-antibodies-ihc-testing-2.jpgAnti-BCL6 Antibody Picoband®IHC analysis of BCL6 using anti-BCL6 antibody (A00142-1). <br>BCL6 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL6 Antibody (A00142-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00142-1-bcl6-primary-antibodies-ihc-testing-1.jpgAnti-BCL6 Antibody Picoband®IHC analysis of BCL6 using anti-BCL6 antibody (A00142-1). <br>BCL6 was detected in a paraffin-embedded section of human B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL6 Antibody (A00142-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00142-1-bcl6-primary-antibodies-if-testing-1.jpgAnti-BCL6 Antibody Picoband®IF analysis of BCL6 using anti-BCL6 antibody (A00142-1) and anti-Alpha Tubulin antibody (M03989-3). <br>BCL6 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BCL6 Antibody (A00142-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd272-btla-picoband-trade-antibody-a03149-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03149-CD272-primary-antibodies-WB-testing-1.jpgAnti-CD272/BTLA Antibody Picoband® Western blot analysis of CD272 using anti-CD272 antibody (A03149).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HEK293 whole cell lysate,<br>Lane 2: human Jurkat whole cell lysate,<br>Lane 3: human CCRF-CEM whole cell lysate,<br>Lane 4: mouse thymus tissue lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD272 antigen affinity purified polyclonal antibody (Catalog # A03149) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD272 at approximately 65KD. The expected band size for CD272 is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03149-41419_2024_6571_fig6_html.pngAnti-CD272/BTLA Antibody Picoband®The comprehensive immunosuppressive mechanism in imatinib resistant GIST. a Cell communication analysis on TIGIT-NECTIN2 and BTLA-TNFRSF14 pair between different cell types in imatinib resistant and sensitive patients respectively. Expression of NECTIN2 ( b ), BTLA ( c ) and TNFRSF14 ( d ) in each cell type in imatinib resistant and sensitive patients respectively. e IHC analysis of NECTIN2, BTLA and TNFRSF14 between imatinib resistant (upper) and sensitive (bottom) patients. f Schematic diagram of the unique tumor-immune microenvironment of imatinib-resistance in advanced GIST. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-06571-3'>38443340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03149-cd272-primary-antibodies-ihc-testing-2.jpgAnti-CD272/BTLA Antibody Picoband® IHC analysis of CD272/BTLA using anti-CD272/BTLA antibody (A03149).<br> CD272/BTLA was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD272/BTLA Antibody (A03149) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03149-cd272-primary-antibodies-ihc-testing-3.jpgAnti-CD272/BTLA Antibody Picoband® IHC analysis of CD272/BTLA using anti-CD272/BTLA antibody (A03149).<br> CD272/BTLA was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD272/BTLA Antibody (A03149) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03149-cd272-primary-antibodies-ihc-testing-4.jpgAnti-CD272/BTLA Antibody Picoband® IHC analysis of CD272/BTLA using anti-CD272/BTLA antibody (A03149).<br> CD272/BTLA was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD272/BTLA Antibody (A03149) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03149-cd272-primary-antibodies-fc-testing-5.pngAnti-CD272/BTLA Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-CD272/BTLA antibody (A03149). <br> Overlay histogram showing THP-1 cells stained with A03149 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD272/BTLA Antibody (A03149,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd84-picoband-trade-antibody-a04872-2-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04872-2-cd84-primary-antibodies-fc-testing-1.pngAnti-CD84 Antibody Flow Cytometry analysis of THP-1 cells using anti-CD84 antibody (A04872-2). <br> Overlay histogram showing THP-1 cells stained with A04872-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04872-2-cd84-primary-antibodies-fc-testing-2.pngAnti-CD84 Antibody Flow Cytometry analysis of JK cells using anti-CD84 antibody (A04872-2). <br> Overlay histogram showing JK cells stained with A04872-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04872-2-cd84-primary-antibodies-ihc-testing-3.jpgAnti-CD84 Antibody IHC analysis of CD84 using anti-CD84 antibody (A04872-2). <br>
CD84 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD84 Antibody (A04872-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-clec9a-picoband-trade-antibody-a04577-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04577-clec9a-primary-antibodies-fc-testing-1.pngAnti-CLEC9A Antibody Flow Cytometry analysis of Jurkat cells using anti-CLEC9A antibody (A04577). <br> Overlay histogram showing Jurkat cells stained with A04577 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC9A Antibody (A04577, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04577-clec9a-primary-antibodies-ihc-testing-1.jpgAnti-CLEC9A AntibodyIHC analysis of CLEC9A using anti-CLEC9A antibody (A04577). <br>CLEC9A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLEC9A Antibody (A04577) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-collagen-iii-col3a1-picoband-trade-antibody-a00788-3-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen III/COL3A1 Antibody Picoband® Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse skin tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of human gastric poorly differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-3.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-4.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-5.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-6.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-ihc-testing-7.jpgAnti-Collagen III/COL3A1 Antibody Picoband® IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Collagen III/COL3A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen III/COL3A1 Antibody (A00788-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00788-3-col3a1-primary-antibodies-fcm-testing-8.jpgAnti-Collagen III/COL3A1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Collagen III/COL3A1 antibody (A00788-3). <br>
Overlay histogram showing Hela cells stained with A00788-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen III/COL3A1 Antibody (A00788-3, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-aromatase-cyp19a1-picoband-trade-antibody-a00071-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00071-Aromatase-primary-antibodies-WB-testing-1.jpgAnti-Aromatase/CYP19A1 Antibody Picoband® Western blot analysis of Aromatase using anti-Aromatase antibody (A00071).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase antigen affinity purified polyclonal antibody (Catalog # A00071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase at approximately 58KD. The expected band size for Aromatase is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-12958_2004_article_143_fig3_html.jpgAnti-Aromatase/CYP19A1 Antibody Picoband®The aromatase activities in SA adipose and liver tissues of the INT (n = 12), INT+EA (n = 12), OVX (n = 12) and OVX+EA (n = 10) rats. ***p < 0.01 vs INT, INT+EA and OVX. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1477-7827-2-18'>15113414</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-12958_2004_article_143_fig4_html.jpgAnti-Aromatase/CYP19A1 Antibody Picoband®Effects of the electroacupuncture on the aromatase expressions by Western blot analysis. The upper picture shows the Western blot analysis of the aromatase P450. The SA adipose and liver tissue samples (50 mg/lane) were electrophoresed and blotted onto the membrane, and aromatase was then detected using the polyclonal antibody as described in materials and methods. Densitometric analysis of the protein concentration using aromatase/β-actin expressed as the mean with SEM bar (n INT = 12, n INT+EA = 12, n OVX = 12 and n OVX+EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT, INT+EA and OVX, # p < 0.05 vs OVX <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1477-7827-2-18'>15113414</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-12958_2004_article_143_fig5_html.jpgAnti-Aromatase/CYP19A1 Antibody Picoband®Effects of the electroacupuncture on the aromatase mRNA expressions by RT-PCR analysis. The upper picture shows the gel electrophoresis of the RT-PCR products for the aromatase. Total RNA fractions were isolated from the SA adipose and liver tissues of the INT, INT+EA, OVX and OVX+EA rats, and the cDNAs were prepared using standard techniques, as described in materials and methods. The RT-PCR products for aromatase were fractionated by electrophoresis through 2% agarose gels. Densitometric analysis of the mRNA concentration using aromatase/β-actin expressed as the mean with SEM bar (n INT = 12, n INT + EA = 12, n OVX = 12 and n OVX + EA = 10) in each column indicated in the lower panel. * p < 0.05 vs INT and INT+EA, # p < 0.05 vs OVX. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1477-7827-2-18'>15113414</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-aromatase-primary-antibodies-ihc-testing-2.jpgAnti-Aromatase/CYP19A1 Antibody Picoband® IHC analysis of Aromatase using anti-Aromatase antibody (A00071). <br> Aromatase was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aromatase Antibody (A00071) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-aromatase-primary-antibodies-ihc-testing-3.jpgAnti-Aromatase/CYP19A1 Antibody Picoband® IHC analysis of Aromatase using anti-Aromatase antibody (A00071). <br> Aromatase was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aromatase Antibody (A00071) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-aromatase-primary-antibodies-ihc-testing-4.jpgAnti-Aromatase/CYP19A1 Antibody Picoband® IHC analysis of Aromatase using anti-Aromatase antibody (A00071). <br> Aromatase was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aromatase Antibody (A00071) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-aromatase-primary-antibodies-ihc-testing-5.jpgAnti-Aromatase/CYP19A1 Antibody Picoband® IHC analysis of Aromatase using anti-Aromatase antibody (A00071). <br> Aromatase was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aromatase Antibody (A00071) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-aromatase-primary-antibodies-ihc-testing-6.pngAnti-Aromatase/CYP19A1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Aromatase antibody (A00071). <br> Overlay histogram showing U20S cells stained with A00071 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aromatase Antibody (A00071,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-dis3-antibody-a01736-1-boster.html2026-03-24T05:20:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01736-1-dis3-primary-antibodies-wb-testing-1.jpgAnti-DIS3 Antibody Picoband® Western blot analysis of DIS3 using anti-DIS3 antibody (A01736-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Raji whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: rat testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIS3 antigen affinity purified polyclonal antibody (Catalog # A01736-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIS3 at approximately 109 kDa. The expected band size for DIS3 is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01736-1-dis3-primary-antibodies-if-testing-2.jpgAnti-DIS3 Antibody Picoband® IF analysis of DIS3 using anti-DIS3 antibody (A01736-1) and anti-Beta Tubulin antibody (M01857-3).<br>
DIS3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DIS3 Antibody (A01736-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01736-1-dis3-primary-antibodies-if-testing-1.jpgAnti-DIS3 Antibody Picoband®IF analysis of DIS3 using anti-DIS3 antibody (A01736-1). <br>
DIS3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DIS3 Antibody (A01736-1) overnight at 4°C. DyLight®550 Conjugated Donkey Anti-Rabbit IgG (BA1144) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01736-1-dis3-primary-antibodies-fcm-testing-3.jpgAnti-DIS3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-DIS3 antibody (A01736-1). <br>
Overlay histogram showing MCF-7 cells stained with A01736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIS3 Antibody (A01736-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01736-1-dis3-primary-antibodies-ip-testing-4.jpgAnti-DIS3 Antibody Picoband® Immunoprecipitating DIS3 in Hela whole cell lysate.<br>
Western blot analysis of DIS3 using anti-DIS3 antibody (A01736-1); <br>
Lane 1: Hela whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-DIS3 antibody in Hela whole cell lysate;<br>
Lane 3: anti-DIS3 antibody (2μg) + Hela whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DIS3 antigen affinity purified polyclonal antibody (A01736-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for DIS3 at approximately 109 kDa. The expected band size for DIS3 is at 109 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-fgf2-picoband-trade-antibody-a00121-2-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-2-fgf2-primary-antibodies-wb-testing-1_1.jpgAnti-FGF2 Antibody Picoband® Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (A00121-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17 kDa. The expected band size for FGF2 is at 17 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-fgf23-picoband-trade-antibody-a00478-3-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00478-3-fgf23-primary-antibodies-wb-testing-1_1.jpgAnti-FGF23 Antibody Picoband® Western blot analysis of FGF23 using anti-FGF23 antibody (A00478-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysate,<br>Lane 2: rat PC-12 whole cell lysate,<br>Lane 3: mouse testis tissue lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody (Catalog # A00478-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-grancalcin-gca-picoband-trade-antibody-a01237-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-wb-testing-1.jpgAnti-Grancalcin/GCA Antibody Picoband® Western blot analysis of Grancalcin/GCA using anti-Grancalcin/GCA antibody (A01237). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human HL-60 whole cell lysates,<br>
Lane 3: human HEL whole cell lysates,<br>
Lane 4: rat testis tissue lysates,<br>
Lane 5: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Grancalcin/GCA antigen affinity purified polyclonal antibody (Catalog # A01237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Grancalcin/GCA at approximately 25 kDa. The expected band size for Grancalcin/GCA is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-ihc-testing-2.jpgAnti-Grancalcin/GCA Antibody Picoband® IHC analysis of Grancalcin/GCA using anti-Grancalcin/GCA antibody (A01237). <br>
Grancalcin/GCA was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Grancalcin/GCA Antibody (A01237) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-ihc-testing-3.jpgAnti-Grancalcin/GCA Antibody Picoband® IHC analysis of Grancalcin/GCA using anti-Grancalcin/GCA antibody (A01237). <br>
Grancalcin/GCA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Grancalcin/GCA Antibody (A01237) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-ihc-testing-4.jpgAnti-Grancalcin/GCA Antibody Picoband® IHC analysis of Grancalcin/GCA using anti-Grancalcin/GCA antibody (A01237). <br>
Grancalcin/GCA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Grancalcin/GCA Antibody (A01237) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-if-testing-5.jpgAnti-Grancalcin/GCA Antibody Picoband® IF analysis of Grancalcin/GCA using anti-Grancalcin/GCA antibody (A01237).<br>
Grancalcin/GCA was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Grancalcin/GCA Antibody (A01237) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01237-gca-primary-antibodies-fcm-testing-6.jpgAnti-Grancalcin/GCA Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Grancalcin/GCA antibody (A01237). <br>
Overlay histogram showing HL-60 cells stained with A01237 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Grancalcin/GCA Antibody (A01237, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-hyaluronan-synthase-1-has1-picoband-trade-antibody-a04784-1-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-wb-testing-1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® Western blot analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human SHG-44 whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat smooth muscle tissue lysates,<br> Lane 5: rat ovary tissue lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse smooth muscle tissue lysates,<br> Lane 8: mouse ovary tissue lysates,<br> Lane 9: mouse small intestine tissue lysates,<br> Lane 10: mouse Neuro-2a whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAS1 antigen affinity purified polyclonal antibody (Catalog # A04784-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAS1 at approximately 70KD. The expected band size for HAS1 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-ihc-testing-2_1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-ihc-testing-3_1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-ihc-testing-4_1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-ihc-testing-5_1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-ihc-testing-6_1.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IHC analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-if-testing-7.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IF analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04784-1-has1-primary-antibodies-if-testing-8.jpgAnti-Hyaluronan synthase 1/HAS1 Antibody Picoband® IF analysis of HAS1 using anti-HAS1 antibody (A04784-1). <br> HAS1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HAS1 Antibody (A04784-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-hla-class-ii-drb1-hla-drb1-picoband-trade-antibody-a00568-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00568-hla-class-ii-drb1-primary-antibodies-wb-testing-1.jpgAnti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Raji whole cell lysate,<br>Lane 2: human Raji whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cd229-ly9-picoband-trade-antibody-a05830-1-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-ihc-testing-1.jpgAnti-CD229/LY9 Antibody IHC analysis of CD229 using anti-CD229 antibody (A05830-1).<br> CD229 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD229 Antibody (A05830-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-ihc-testing-2.jpgAnti-CD229/LY9 Antibody IHC analysis of CD229 using anti-CD229 antibody (A05830-1).<br> CD229 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD229 Antibody (A05830-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-ihc-testing-3.jpgAnti-CD229/LY9 Antibody IHC analysis of CD229 using anti-CD229 antibody (A05830-1).<br> CD229 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD229 Antibody (A05830-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-ihc-testing-4.jpgAnti-CD229/LY9 Antibody IHC analysis of CD229 using anti-CD229 antibody (A05830-1).<br> CD229 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD229 Antibody (A05830-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-fcm-testing-5.pngAnti-CD229/LY9 Antibody Flow Cytometry analysis of Jurkat cells using anti-CD229 antibody (A05830-1).<br> Overlay histogram showing Jurkat cells stained with A05830-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD229 Antibody (A05830-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-fcm-testing-6.pngAnti-CD229/LY9 Antibody Flow Cytometry analysis of Daudi cells using anti-CD229 antibody (A05830-1).<br> Overlay histogram showing Daudi cells stained with A05830-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD229 Antibody (A05830-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05830-1-cd229-primary-antibodies-fcm-testing-7.pngAnti-CD229/LY9 Antibody Flow Cytometry analysis of U937 cells using anti-CD229 antibody (A05830-1).<br> Overlay histogram showing U937 cells stained with A05830-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD229 Antibody (A05830-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-myelin-oligodendrocyte-glycoprotein-mog-picoband-trade-antibody-a03294-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-wb-testing-1_2.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® Western blot analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin oligodendrocyte glycoprotein antigen affinity purified polyclonal antibody (Catalog # A03294) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin oligodendrocyte glycoprotein at approximately 26KD. The expected band size for Myelin oligodendrocyte glycoprotein is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-mog-primary-antibodies-ihc-testing-7.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband®IHC analysis of MOG using anti-MOG antibody (A03294). <br>MOG was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOG Antibody (A03294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-ihc-testing-2.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).<br> Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-ihc-testing-3.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).<br> Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-ihc-testing-4.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).<br> Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-ihc-testing-5.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).<br> Myelin oligodendrocyte glycoprotein was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-mog-primary-antibodies-ihc-testing-6.jpgAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® IHC analysis of Myelin oligodendrocyte glycoprotein using anti-Myelin oligodendrocyte glycoprotein antibody (A03294). <br>
Myelin oligodendrocyte glycoprotein was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03294-myelin-oligodendrocyte-glycoprotein-primary-antibodies-fcm-testing-7.pngAnti-Myelin oligodendrocyte glycoprotein/MOG Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Myelin oligodendrocyte glycoprotein antibody (A03294).<br> Overlay histogram showing U251 cells stained with A03294 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Myelin oligodendrocyte glycoprotein Antibody (A03294,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pcdh15-picoband-trade-antibody-a03591-1-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03591-1-PCDH15-primary-antibodies-WB-testing-1.jpgAnti-PCDH15 Antibody Picoband® Western blot analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysate,<br>Lane 2: human U2OS whole cell lysate,<br>Lane 3: human Hela whole cell lysate,<br>Lane 4: rat brain tissue lysate,<br>Lane 5: rat lung tissue lysate,<br>Lane 6: mouse brain tissue lysate,<br>Lane 7: mouse lung tissue lysate,<br>Lane 8: mouse kidney tissue lysate,<br>Lane 9: mouse spleen tissue lysate,<br>Lane 10: mouse Neuro-2a whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDH15 antigen affinity purified polyclonal antibody (Catalog # A03591-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDH15 at approximately 190KD. The expected band size for PCDH15 is at 216KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-2.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-3.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-4.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-5.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-6.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-7.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-8.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-ihc-testing-9.jpgAnti-PCDH15 Antibody Picoband® IHC analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).<br> PCDH15 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCDH15 Antibody (A03591-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03591-1-pcdh15-primary-antibodies-fc-testing-10.pngAnti-PCDH15 Antibody Picoband®10. Flow Cytometry analysis of U-87MG cells using anti-PCDH15 antibody (A03591-1). <br> Overlay histogram showing U-87MG cells stained with A03591-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCDH15 Antibody (A03591-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pgf-picoband-trade-antibody-a01164-2-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01164-2-pgf--primary-antibodies-wb-testing-1.jpgAnti-PGF Antibody Picoband® Western blot analysis of PGF using anti-PGF antibody (A01164-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human T-47D whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGF antigen affinity purified polyclonal antibody (Catalog # A01164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGF at approximately 25KD. The expected band size for PGF is at 25KD.
https://www.bosterbio.com/products/primary-antibodies/anti-upa-receptor-plaur-picoband-trade-antibody-a00993-3-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00993-3-uPA-Receptor-primary-antibodies-WB-testing-1.jpgAnti-uPA Receptor/PLAUR Antibody Picoband® Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysate,<br>Lane 2: human U20S whole cell lysate,<br>Lane 3: human A431 whole cell lysate,<br>Lane 4: human Hela whole cell lysate,<br>Lane 5: human A549 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (Catalog # A00993-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00993-3-uPA-Receptor-primary-antibodies-WB-testing-2.jpgAnti-uPA Receptor/PLAUR Antibody Picoband® Western blot analysis of uPA Receptor using anti-uPA Receptor antibody (A00993-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysate,<br>Lane 2: mouse small intestine tissue lysate,<br>Lane 3: mouse kidney tissue lysate,<br>Lane 4: mouse testis tissue lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody (Catalog # A00993-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-3-plaur-primary-antibodies-fcm-testing-3.jpgAnti-uPA Receptor/PLAUR Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-uPA Receptor antibody (A00993-3). <br>
Overlay histogram showing SiHa cells stained with A00993-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-uPA Receptor Antibody (A00993-3, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-melanoma-gp100-pmel-picoband-trade-antibody-a01262-1-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-1-pmel-primary-antibodies-wb-testing-1.jpgAnti-Melanoma gp100/PMEL Antibody Picoband®Western blot analysis of SILV/PMEL using anti-SILV/PMEL antibody (A01262-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A2058 whole cell lysates,<br>
Lane 2: rat testis tissue lysates,<br>
Lane 3: rat C6 whole cell lysates,<br>
Lane 4: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SILV/PMEL antigen affinity purified polyclonal antibody (Catalog # A01262-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SILV/PMEL at approximately 80 kDa. The expected band size for SILV/PMEL is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-1-pmel-primary-antibodies-ihc-testing-2.jpgAnti-Melanoma gp100/PMEL Antibody Picoband®IHC analysis of SILV/PMEL using anti-SILV/PMEL antibody (A01262-1). <br>
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SILV/PMEL Antibody (A01262-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-1-pmel-primary-antibodies-ihc-testing-3.jpgAnti-Melanoma gp100/PMEL Antibody Picoband®IHC analysis of SILV/PMEL using anti-SILV/PMEL antibody (A01262-1). <br>
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SILV/PMEL Antibody (A01262-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-1-pmel-primary-antibodies-if-testing-5.jpgAnti-Melanoma gp100/PMEL Antibody Picoband®IF analysis of SILV/PMEL using anti-SILV/PMEL antibody (A01262-1). <br>
SILV/PMEL was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SILV/PMEL Antibody (A01262-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-1-pmel-primary-antibodies-fcm-testing-4.pngAnti-Melanoma gp100/PMEL Antibody Picoband®Flow Cytometry analysis of U20S cells using anti-SILV/PMEL antibody (A01262-1). <br>
Overlay histogram showing U20S cells stained with A01262-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SILV/PMEL Antibody (A01262-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pkc-beta-1-prkcb-picoband-trade-antibody-a01940-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01940-prkcb-primary-antibodies-wb-testing-1.jpgAnti-PKC beta 1/PRKCB Antibody Picoband® Western blot analysis of PRKCB using anti-PRKCB antibody (A01940). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCB antigen affinity purified polyclonal antibody (Catalog # A01940) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCB at approximately 77 kDa. The expected band size for PRKCB is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01940-prkcb-primary-antibodies-ihc-testing-2.jpgAnti-PKC beta 1/PRKCB Antibody Picoband® IHC analysis of PRKCB using anti-PRKCB antibody (A01940). <br>
PRKCB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKCB Antibody (A01940) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01940-prkcb-primary-antibodies-fcm-testing-3.jpgAnti-PKC beta 1/PRKCB Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-PRKCB antibody (A01940). <br>
Overlay histogram showing Jurkat cells stained with A01940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKCB Antibody (A01940, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pkc-gamma-prkcg-picoband-trade-antibody-a01890-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-wb-testing-1.jpgAnti-PKC gamma/PRKCG Antibody Picoband® Western blot analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: mouse brain tissue lysates, <br>
Lane 4: rat kidney tissue lysates, <br>
Lane 5: mouse kidney tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC gamma antigen affinity purified polyclonal antibody (Catalog # A01890) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC gamma at approximately 78 kDa. The expected band size for PKC gamma is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-2.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890).<br> PKC gamma was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-3.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890).<br> PKC gamma was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-4.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890).<br> PKC gamma was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-5.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890).<br> PKC gamma was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-6.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
PKC gamma was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-7.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
PKC gamma was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-ihc-testing-8.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IHC analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
PKC gamma was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-if-testing-9.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
PKC gamma was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-if-testing-10.jpgAnti-PKC gamma/PRKCG Antibody Picoband® IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). <br>
PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01890-pkc-gamma-primary-antibodies-if-testing-11.jpg.jpgAnti-PKC gamma/PRKCG Antibody Picoband® PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. HRP conjugated goat anti-rabbit IgG (BA1054) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using TSA-550 Conjugated. GFAP was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. HRP conjugated goat anti-rabbit IgG (BA1054) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using TSA-488 Conjugated. The sections were counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-peptide-yy-pyy-picoband-trade-antibody-a04223-1-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04223-1-pyy-primary-antibodies-ihc-testing-5.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY/PYY using anti-Peptide YY/PYY antibody (A04223-1). Peptide YY/PYY was detected in paraffin-embedded section of human appendicitis tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY/PYY Antibody (A04223-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04223-1-pyy-primary-antibodies-ihc-testing-2_1.jpgAnti-Peptide YY/PYY AntibodyIHC analysis of Peptide YY/PYY using anti-Peptide YY/PYY antibody (A04223-1). <br>Peptide YY/PYY was detected in a paraffin-embedded section of human small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Peptide YY/PYY Antibody (A04223-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-si-picoband-trade-antibody-a04542-1-boster.html2026-04-01T05:01:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04542-1-si-primary-antibodies-wb-testing-1.jpgAnti-SI Antibody Picoband® Western blot analysis of SI using anti-SI antibody (A04542-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat small intestine tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SI antigen affinity purified polyclonal antibody (A04542-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SI at approximately 209 kDa. The expected band size for SI is at 209 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04542-1-si-primary-antibodies-ihc-testing-2_1.jpgAnti-SI Antibody Picoband® IHC analysis of SI using anti-SI antibody (A04542-1). <br>SI was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SI Antibody (A04542-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04542-1-si-primary-antibodies-ihc-testing-3_1.jpgAnti-SI Antibody Picoband® IHC analysis of SI using anti-SI antibody (A04542-1). <br>SI was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SI Antibody (A04542-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04542-1-si-primary-antibodies-fcm-testing-4.jpgAnti-SI Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-SI antibody (A04542-1). <br>
Overlay histogram showing CACO-2 cells stained with A04542-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SI Antibody (A04542-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sri-picoband-trade-antibody-a00222-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-sr1-primary-antibodies-wb-testing-1.jpgAnti-SRI Antibody Picoband® Western blot analysis of SR1 using anti-SR1 antibody (A00222). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysate,<br>Lane 2: human U20S whole cell lysate,<br>Lane 3: human A431 whole cell lysate,<br>Lane 4: human PC-3 whole cell lysate,<br>Lane 5: human HL-60 whole cell lysate,<br>Lane 6: human K562 whole cell lysate,<br>Lane 7: human Caco-2 whole cell lysate,<br>Lane 8: rat lung tissue lysate,<br>Lane 9: mouse lung tissue lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SR1 antigen affinity purified polyclonal antibody (Catalog # A00222) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SR1 at approximately 22KD. The expected band size for SR1 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-sr1-primary-antibodies-ihc-testing-2_1.jpgAnti-SRI Antibody Picoband® IHC analysis of SRI using anti-SRI antibody (A00222).<br> SRI was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRI Antibody (A00222) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-sr1-primary-antibodies-ihc-testing-3_1.jpgAnti-SRI Antibody Picoband® IHC analysis of SRI using anti-SRI antibody (A00222).<br> SRI was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRI Antibody (A00222) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-sr1-primary-antibodies-ihc-testing-4_1.jpgAnti-SRI Antibody Picoband® IHC analysis of SRI using anti-SRI antibody (A00222).<br> SRI was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRI Antibody (A00222) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-5.jpgAnti-SRI Antibody Picoband® IF analysis of SRI using anti-SRI antibody (A00222) <br> SRI was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SRI Antibody (A00222) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-6.pngAnti-SRI Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SRI antibody (A00222). <br> Overlay histogram showing SiHa cells stained with A00222 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRI Antibody (A00222,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-7.pngAnti-SRI Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SRI antibody (A00222). <br> Overlay histogram showing U20S cells stained with A00222 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRI Antibody (A00222,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-thrombopoietin-thpo-picoband-trade-antibody-a03222-3-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03222-3-Thrombopoietin-primary-antibodies-WB-testing-1.jpgAnti-Thrombopoietin/THPO Antibody Picoband® Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HepG2 whole cell lysate,<br>Lane 2: human Caco-2 whole cell lysate,<br>Lane 3: rat liver tissue lysate,<br>Lane 4: mouse liver tissue lysate,
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.
https://www.bosterbio.com/picokine-elisa-kits/human-itih4-picokine-trade-elisa-kit-ek1670-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1670.pngHuman ITIH4 ELISA Kit PicoKine®Human ITIH4 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd14-picokine-trade-elisa-kit-ek0694-boster.html2026-03-24T05:20:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0694.pngHuman CD14 ELISA Kit PicoKine®Human CD14 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-il6st-picokine-trade-elisa-kit-ek1742-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1742.jpgRat IL6ST ELISA Kit PicoKine®Rat IL6ST PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-ckmm-picokine-trade-elisa-kit-ek1751-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1751.jpgHuman ckmm ELISA Kit PicoKine®Human ckmm PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-vwf-a2-picokine-trade-elisa-kit-ek1743-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1743.jpgHuman VWF-A2/VWF ELISA Kit PicoKine®Human VWF-A2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-endorepellin-hspg2-picokine-trade-elisa-kit-ek1760-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1760.jpgHuman Endorepellin/HSPG2 ELISA Kit PicoKine®Human Endorepellin/HSPG2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-amica-jaml-picokine-trade-elisa-kit-ek1730-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1730.pngHuman AMICA/JAML ELISA Kit PicoKine®Human AMICA/JAML PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-ki67-picokine-trade-elisa-kit-ek1759-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1759.jpgHuman Ki67 ELISA Kit PicoKine®Human Ki67 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-pros1-picokine-trade-elisa-kit-ek1739-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1739.jpgHuman PROS1 / Protein S ELISA Kit PicoKine®Human PROS1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cxadr-picokine-trade-elisa-kit-ek1725-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1725.jpgMouse CXADR ELISA Kit PicoKine®Mouse CXADR PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-cxadr-picokine-trade-elisa-kit-ek1726-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1726.jpgRat CXADR ELISA Kit PicoKine®Rat CXADR PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-glypican-5-gpc5-picokine-trade-elisa-kit-ek1738-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1738.pngHuman Glypican-5/GPC5 ELISA Kit PicoKine®Human Glypican-5/GPC5 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-bmp-10-picokine-trade-elisa-kit-ek1808-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1808.jpgHuman BMP-10 ELISA Kit PicoKine®Human BMP-10 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-bmp-10-picokine-trade-elisa-kit-ek1809-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1809.pngMouse BMP-10 ELISA Kit PicoKine®Mouse BMP-10 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-bmp-10-picokine-trade-elisa-kit-ek1810-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1810.pngRat BMP-10 ELISA Kit PicoKine®Rat BMP-10 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-annexin-a1-picokine-trade-elisa-kit-ek1745-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1745.pngHuman Annexin A1 ELISA Kit PicoKine®Human Annexin A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf12a-tweakr-picokine-trade-elisa-kit-ek1753-boster.html2026-03-30T05:00:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1753.pngHuman TNFRSF12A/TWEAKR ELISA Kit PicoKine®Human TNFRSF12A/TWEAKR PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-contactin-1-picokine-trade-elisa-kit-ek1771-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1771.pngHuman Contactin-1 ELISA Kit PicoKine®Human Contactin-1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-robo1-picokine-trade-elisa-kit-ek1755-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1755.pngHuman ROBO1 ELISA Kit PicoKine®Human ROBO1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-epha1-picokine-trade-elisa-kit-ek1796-boster.html2026-03-24T05:20:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1796.pngHuman EphA1 ELISA Kit PicoKine®Human EphA1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-contactin-1-picokine-trade-elisa-kit-ek1772-boster.html2026-03-24T05:20:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1772.pngMouse Contactin-1 ELISA Kit PicoKine®Mouse Contactin-1 PicoKine ELISA Kit standard curve
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https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-alpha-antibody-pa1079-boster.html2026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079_41467_2025_61914_fig6_html.pngAnti-TNF alpha Antibody Picoband®Immunohistochemical and neovascularization analysis of wound tissue in mice. A, B Representative images of immunohistochemistry staining for IL-6 and TNF-α, respectively. n = 6 independent samples. C Neovascularization in different groups. D–G Quantified data of the relative area coverage of IL-6, TNF-α, α-SMA, and CD31, respectively. n = 6 independent samples in results ( D ) and ( E ), while n = 5 independent samples in results ( F ) and ( G ). For the data in Fig. , * p < 0.05, **** p < 0.0001, the exact P value between CSFCM + M and CSFCM group was 0.0356. For the data in Fig. , * p < 0.05, ** p < 0.01, **** p < 0.0001, the exact P value between CSFCM + M and CM group was 0.006, the exact P value between CSFCM + M and CSF group was 0.0068, the exact P value between CSFCM + M and CSFCM group was 0.0086, the exact P value between CSFCM + M and CSF + M group was 0.0108. For the data in Fig. , * p < 0.05, ** p < 0.01, **** p < 0.0001, the exact P value between CSFCM + M and PBS group was 0.0128, the exact P value between CSFCM + M and CSF group was 0.0185, and the exact P value between CSFCM + M and CSFCM group was 0.0054. For the data in Fig. , * p < 0.05, ** p < 0.01, **** p < 0.0001, the exact P value between CSFCM + M and PBS group was 0.0343, the exact P value between CSFCM + M and CM group was 0.0155, the exact P value between CSFCM + M and CSF group was 0.0197, the exact P value between CSFCM + M and CSFCM group was 0.008. Data in D – G were presented as mean ± S.D. P values were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61914-8'>40695799</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079_1-s2.0-s2405580825002407-gr4.jpgAnti-TNF alpha Antibody Picoband®SADS inhibited Raw264.7 and fibroblast-like synoviocyte inflammation in vitro. (A) The effect of SADS on the viability of Raw264.7. (B–D) RT-PCR analysis of TNF-α, IL-6 and IL-10 in Raw264.7 treated with SADS.(E) The effect of SADS on the viability of fibroblast-like synoviocytes. (F–H) RT-PCR analysis of TNF-α, IL-6 and IL-10 in fibroblast-like synoviocytes treated with SADS.(I) The phenotype of Raw264.7 was analyzed by flow cytometry.(J and K) Statistics of the proportion of M2 and M1 macrophages. (L and M) TNF-α protein expression level detection. ##P < 0.01 versus Ctrl; ∗∗P < 0.01versus LPS. n = 6.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2405580825002407'>40688514</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079_1-s2.0-s2405580825002407-gr6.jpgAnti-TNF alpha Antibody Picoband®Pathological evaluation of IL-1RA−/− mice synovial tissue. HE staining showed synovial hyperplasia (A) and inflammation (B). (C) IHC staining of TNF-α in synovial tissue. (D) IHC staining of IL-10 in synovial tissue.(E and F) Levels of TNF-α and IL-10 in the blood of IL1RA−/ -deficient mice. ##P < 0.01 versus Ctrl; ∗∗P < 0.01versus Model. n = 6 mice for each group. Control is wild-type mice, and the model is IL1RA−/− mice.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2405580825002407'>40688514</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-dddt_a_12305505_f0004_c.jpgAnti-TNF alpha Antibody Picoband®(A) Immunohistochemical images of IL-1β, IL-6, TNF-α, and NF-κB; Mean density: (B) IL-1β; (C) IL-6; (D) TNF-α; (E) NF-κB; (F) Western blot of IL-1β, IL-6, and TNF-α expression; (G) Peripheral blood IL-1β content. Data are presented as mean ± SD. Vs NC, ##P <0.01; Vs PCOS, *P <0.05, **P <0.01; n = 6 per group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DDDT.S484531'>39247793</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-fbioe-13-1688905-g006.jpgAnti-TNF alpha Antibody Picoband®Immunomodulatory properties pathway of the hydrogel. (A, B) Flow cytometry analysis of the macrophage surface markers CD11C and CD86. (C) Representative immunofluorescence images of iNOS in RAW264.7 cells. (D, E) Quantitative analysis of flow cytometry in CD11C and CD86. (F) Quantitative analysis of the mean fluorescence intensity of iNOS. (G) Western blot of IκB-α, p65, and p-p65. (H) Quantitative analysis of the relative expression of IκB-α, p65, and p-p65 in different hydrogels.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1688905/full'>41190287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-fbioe-13-1688905-g007.jpgAnti-TNF alpha Antibody Picoband®Wound healing with different hydrogels in vivo . (A) Preparation and observation of diabetic chronic wounds related to orthopedics. (B) Photographs of the wound treated with the hydrogel in different hydrogel groups. (C) Wound recovery curve of different hydrogel groups. (D) H&E staining and Masson staining of wound tissue on day 10 in different hydrogel groups. (E) IHC staining of wound tissue in iNOS and CD206.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1688905/full'>41190287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-13075_2018_1594_fig4_html.pngAnti-TNF alpha Antibody Picoband®Influence of the invasion of pannus in the cartilage area on cartilaginous structures and related inflammation expression (10 × 20 magnification). a - e In sacroiliitis, abundant fibrovascular tissue formed and invaded into cartilage, chondrocytes and matrix degenerated ( a ), accompanied by high levels of vascular endothelial growth factor (VEGF) ( b ), caspase-3 ( c ), matrix metalloproteinase-3 MMP-3 ( d ) and TNF-α ( e ) expressed in the cartilage. f - j In sacroiliitis, fibrovascular tissue formed in the subchondral area without invading into cartilage ( f ), accompanied by significantly lower levels of VEGF ( g ), caspase-3 ( h ), MMP-3 ( i ) and TNF-α ( j ) expressed in the cartilage. k - o In autopsy controls, there was no fibrovascular tissue formation and only partial superficial chondrocytes expressed low levels of VEGF ( l ), caspase-3 ( m ) and TNF-α ( o ). a , f , k , AP-Red staining; b - e , g - j , l - o , 3,3-diaminobenzidine staining. SpA, spondyloarthritis <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13075-018-1594-z'>29884210</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-12906_2019_2539_fig8_html.pngAnti-TNF alpha Antibody Picoband®Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 in L -NAME-induced hypertensive mice. ( a ) TNF-α expression level in plasma. ( b ) IL-1β expression level in plasma. ( c ) IL-10 expression level in plasma. ( d ) IL-1β expression level in cardiac tissues. ( e ) TNF-α expression level in cardiac tissues. ( f ) IL-10 expression level in cardiac tissues. ( g ) Representative image of IL-1β immunocytochemistry. ( h ) Representative image of TNF-α immunocytochemistry.( i ) Representative image of IL-10 immunocytochemistry.1,negative group; 2,control group; 3, model group; 4, L -NAME+AqE group; 5, L -NAME+XJEK group; 6, L -NAME+fosinopril group. Data are presented as the mean ± SD ( n = 10). ** P < 0.01 vs. control group; # P < 0.05, ## P < 0.01 vs, model group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12906-019-2539-z'>31196042</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-fmicb-11-622354-g002.jpgAnti-TNF alpha Antibody Picoband®The infiltration of MPO + neutrophils, and the cellular distribution and relative expression level detection of the TNF and IL-10 in the small intestinal and colonic mucosa at 7 days after the termination of DSS administration. (A) The MPO immunohistochemistry staining of the small intestinal mucosa: (A1) the normal group: few neutrophils were observed in the small intestinal mucosa; (A2) the DSS group: a number of accumulative MPO + neutrophils (brown) infiltrated into the mucosa epithelium; (A3) the DSS + B. subtilis- fermented milk group: only limited neutrophil infiltration could be observed in the small intestinal mucosa. (B) The MPO immunohistochemistry staining of the colonic mucosa: (B1) the normal group: few neutrophils were observed in the colonic mucosa; (B2) the DSS group: colonic epithelium and the glands disappeared, and the ulcer was locally replaced by scars and a number of accumulative MPO + neutrophils (brown) were observed in the scars; (B3) the DSS + B. subtilis -fermented milk group: only limited MPO + neutrophils observed in the colonic mucosa. (C) The TNF immunohistochemistry staining of the small intestinal mucosa: (C1) the normal group: the epithelium was integrated with faint yellow staining, suggesting low expression of TNF; (C2) the DSS group: the villus structure is not integrated, and the epithelial cells showed black brown, suggesting overexpression of TNF; (C3) the DSS + B. subtilis -fermented milk group: the villus and the glands were almost integrated, and the staining of epithelial cells was similar to that of the normal group (C1) , suggesting low expression of TNF. (D) The TNF immunohistochemistry staining of the colonic mucosa: (D1) the normal colonic mucosa: the epithelium was integrated with low TNF expression (faint yellow); ( D2 ) the DSS group: the epithelium structure and the glands were destroyed and replaced by a scar, and there were a number of TNF + inflammatory cells (black brown) in the scar; (D3) the DSS + B. subtilis -fermented milk group: the recovered epithelium showed faint yellow, suggesting low TNF expression. (E) The IL-10 immunohistochemistry staining of the small intestinal mucosa: (E1) the normal small intestinal mucosa: the IL-10 staining dispersed in the villi and the crypts with faint yellow, suggesting low-level expression of IL-10; (E2) the DSS group, the residual epithelium and the crypts were light brown, suggesting mid-level of IL-10 expression; (E3) the DSS + B. subtilis -fermented milk group: the dark brown staining of the regenerative epithelium represented high-level expression of IL-10. (F) The IL-10 immunohistochemistry staining of the colonic mucosa: (F1) the normal group: the IL-10 staining dispersed in the glands with bright yellow, suggesting low-level expression of IL-10; (F2) the DSS group: there were few IL-10 + cells in the scars; (F3) the DSS + B. subtilis -fermented milk group, the dark brown staining of the epithelial cells represented high-level expression of IL-10. (G,H) Western blotting analysis for the expression of MPO, TNF, and IL-10 in the samples containing equivalent ileum and colon. The expression level of MPO, TNF, and IL-10 in the DSS group was significantly higher than that of the normal (control) group. The expression level of MPO and TNF in the DSS + B. subtilis -fermented milk (FM) group was significantly lower than that of the DSS group, while the expression level of IL-10 in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-12906_2019_2539_fig2_html.pngAnti-TNF alpha Antibody Picoband®Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 of HUVECs induced by Ang II. ( a ) TNF-α level in supernatants of HUVECs; ( b ) IL-1β level in supernatants of HUVECs; ( c ) IL-10 level in supernatants of HUVECs. 1, blank control group; 2, Ang II (10 − 5 mol/L) group; 3, Ang II (10 − 5 mol/L) + AqE (0.15 mg/ml) group; 4, Ang II (10 − 5 mol/L) + AqE (0.3 mg/ml) group; 5, Ang II (10 − 5 mol/L) + AqE (0.6 mg/ml) group; 6, Ang II (10 − 5 mol/L) + AqE (1.2 mg/ml) group; 7, Ang II (10 − 5 mol/L) + XJEK (1.6 mg/ml) group. Data are expressed as mean ± SD, n = 6. ** P < 0.01 vs control group; ## P < 0.01 vs Ang II group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12906-019-2539-z'>31196042</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-fphar-15-1430599-g003.jpgAnti-TNF alpha Antibody Picoband®MT inhibited the production of pro-inflammatory proteins in sleep-deprived rats. (A) Western blot bands showing the protein expression levels of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. (B–F) Relative protein expression level of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. The data are expressed as the means ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs Control group; * p < 0.05, ** p < 0.01 vs. Model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1430599/full'>39101143</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-41467_2024_50481_fig8_html.pngAnti-TNF alpha Antibody Picoband®Skin phenotype in Krt32 KO mice induced by TNF. A Schematic representation of the experimental schedule. Eight-week-old Krt32 wildtype (WT) and knockout (KO) C57BL/6J mice ( n = 8 mice/group) were subcutaneously injected with TNF at a dose of 6 μg/kg/day body weight into their shaved dorsal skin for a duration of 48 h. The dorsal skin of Krt32 ( −/−) mice exhibited pronounced thickening and extensive yellow scaling, resembling the cutaneous manifestations observed in human patients with pityriasis rubra pilaris (PRP). Skin, hair, and nail samples were collected for RNA-seq, H&E staining, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) analysis. B , C Two representative photos of the dorsal skin of WT and Krt32 KO mice ( n = 8 mice/group) treated by TNF, along with H&E staining showing lymphocytes and hair follicular plugging indicated by red and black arrows respectively. Results from another six mice are presented in Supplementary Fig. A, B. D Selected KEGG signal transduction pathways identified for significant DEGs (P < 0.05 and log 2 FC > 0.585) through RNA sequencing of between Krt32 WT and KO C57BL/6J mice ( n = 8 mice/group) treated by TNF. The statistical test was hypergeometric test, and the level of significance was set at a two-sided P < 0.05 without multiple comparisons. E Selected NF-κB pathway-associated genes are highlighted. Colored points denote P < 0.05, which means −log ( P ) > 1.30, dashed line), with red indicating upregulated genes (log 2 FC > 0.585) and blue indicating downregulated genes (log 2 FC < −0.585). The statistical test was Wald test, and the level of significance was set at a two-sided P < 0.05 without multiple comparisons. FC denotes fold change. F , G RT-qPCR analysis of Il1b and Il6 expression in the epidermis of Krt32 (−/−) mice ( n = 8) and WT mice ( n = 8) with pre- and post-TNF stimulation. Data are shown as means ± SEM in ( F ) and ( G ). P value was calculated using a two-sided unpaired Student’s t test. Representative immunohistochemical staining of the dorsal skin of Krt32 WT and KO mice treated with TNF for detecting IL-1β ( H ) Ki67 ( I ) and NEMO ( J ). Scale bar = 150 μm. Results from another six mice are presented in Supplementary Fig. C–E. K Western blot analysis of NEMO and p-p65 in the skin tissue lysates from Krt32 ( −/− ) mice ( n = 3) and WT mice ( n = 3) with TNF treatment. L Statistical analysis the expression of NEMO and p-p65 in ( K ). Data are shown as means ± SEM in ( L ). P value was calculated using a two-sided unpaired Student’s t test. M Western blot analysis of NEMO and p-p65 in epidermal cell lysates from a mixture of cells from four Krt32 WT and KO suckling mice with and without TNF treatment. One representative experiment from two independent experiments with similar results is shown in ( M ). Source data are provided as file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-50481-z'>39048559</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-primary-antibodies-wb-testing-1.jpgAnti-TNF alpha Antibody Picoband® Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U937 whole cell lysates,<br>
Lane 2: rat spleen tissue lysates,<br>
Lane 3: rat C6 whole cell lysates,<br>
Lane 4: mouse spleen tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25 kDa. The expected band size for TNF alpha is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-41467_2024_50481_fig7_html.pngAnti-TNF alpha Antibody Picoband®The interaction of KRT32 with NEMO promotes NEMO degradation via K48-linked polyubiquitination modification and also inhibits the formation of the IKK complex. KRT32 expression level does not affect the transcription of IKBKG (the gene encoding NEMO) in Ker-CT cells. The mRNA level of NEMO in Ker-CT cells overexpressing KRT32 detected using RNA-seq ( A ) and in KRT32 knockdown cells detected by RT-qPCR analysis ( B ). Data are means ± SEM of three independent experiments in ( A ) and ( B ), and P value was calculated using a two-sided unpaired Student’s t test. C , D Immunoblotting of NEMO in Ker-CT cells with overexpressing KRT32 and knockdown with and without TNF treatment. E The impact of KRT32 wildtype and mutations overexpression on NEMO protein level in Ker-CT cells analyzed by Western blot. F Cycloheximide (CHX) chase assay to analyze the protein stability of NEMO in Ker-CT cells overexpressing KRT32. Cells were treated with 50 µg/ml CHX for indicated time. G The levels of NEMO were measured by western blotting with 5 μM MG132 in Ker-CT cells overexpressing KRT32 wildtype or negative control for 12 h. H Co-immunoprecipitation of HA-KRT32 and Flag-NEMO in HEK 293T cells, followed by detection of ubiquitin levels of NEMO by Western blotting. I Co-transfection of Flag-NEMO with HA-Ub-WT, HA-Ub-K48 or K48R (containing lysine at residue K48 or lysine to arginine mutation at residue K48) along with Myc-KRT32 in HEK 293 T cells, followed by immunoprecipitation and Western blotting analysis. J Immunoprecipitation of lysates with NEMO antibody from Ker-CT cells overexpressing KRT32 wildtype or mutations, and then detect the Ub-K48 modification of NEMO. PLA analysis in Ker-CT cells overexpression KRT32 wildtype and mutations to assess the interaction of Ub with NEMO ( K ) and IKKα with NEMO ( M ). Scale bars represent 10 μm. L , N Quantification of the number of PLA foci per cell detected in ( K ) and ( M ), separately. Each dot on the graph corresponds to a specific analyzed cell. Red bars represent the mean ± SEM from the indicated number ( N ) of cells. The number of cells analyzed per group varies as follows: N = 315, 302, 302, 310, 302, 315, 313, 311 in ( L ) and N = 243, 211, 214, 220, 217, 214, 205, 208 in ( N ), with each group consisting of three biological replicates. P value was calculated using a two-sided unpaired Student’s t test. O GST-NEMO (120-419aa) fusion protein was incubated with excess E. coli extracts containing His-KRT32 (wildtype or six mutants), His-IKKα, and His-IKKβ. GST complex was pulled down with glutathione-Sepharose beads, and the protein complexes were analyzed by western blotting. P Statistical analysis was performed on the binding ability of IKKα/β and NEMO with wildtype KRT32 and its mutations addition from tree-independent experiments of ( O ). Data shown as means ± SEM of three independent experiments in ( P ). P value was calculated using a two-sided unpaired Student’s t test. Q Immunoblotting assay of phosphorylated IKKα/β in Ker-CT cells overexpressing KRT32 wildtype and mutations. One representative experiment from two independent experiments with similar results is shown in ( C – G ) and ( J , Q ). One representative experiment from three independent experiments with similar results is shown in ( O ). Source data are provided as file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-50481-z'>39048559</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-primary-antibodies-ihc-testing-2.jpgAnti-TNF alpha Antibody Picoband® IHC analysis of TNF alpha using anti-TNF alpha antibody (PA1079). <br>
TNF alpha was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNF alpha Antibody (PA1079) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-41467_2024_50481_fig4_html.pngAnti-TNF alpha Antibody Picoband®KRT32 inhibits the activation of NF-κB signaling pathway. A Selected KEGG signal transduction pathway enrichment analysis with significant DEGs (FDR < 0.05 and log 2 FC < −0.585) through RNA sequencing of Ker-CT cells with KRT32 overexpression. The statistical test was hypergeometric test, and the level of significance was set at a two-sided P < 0.05 without multiple comparisons. B Volcano plot illustrating significant DEGs between KRT32 overexpression and negative control in Ker-CT cells. Upregulated genes were labelled by red dots with FDR < 1e −20 , log 2 FC > 0.585; and downregulated genes was labelled by blue dots with FDR < 1e −20, log 2 FC < −0.585. Selected NF-κB pathway-associated genes are highlighted. FC denotes fold change. The statistical test was Wald test and the level of significance was set at a two-sided FDR < 0.05 with multiple comparisons by Benjamini-Hochberg method. C NF-κB-dependent luciferase activation assay in HEK293T cells to analyze concentration-dependent role of KRT32 in NF-κB activation under the stimulation with TNF (20 ng/mL). D The phosphorylated p65 expression in KRT32 knockdown Ker-CT cells with and without TNF (20 ng/mL) treatment for the indicated time. E – G Detection of IL1β, IL-6, IL8 secretion in supernatant of KRT32 knockdown Ker-CT cells with or without TNF treatment by MSD assay. H – I The phosphorylated p65 expression in primary keratinocytes and Ker-CT cells with Flag-tagged KRT32 overexpression. J – L MSD assay detection of IL1β, IL-6, IL8 secretion of Ker-CT cells overexpressing KRT32. M The phosphorylated p65 expression in Ker-CT cells overexpressing Flag-tagged KRT32 wildtype and mutations. N , O MSD assay detection of IL1β, IL-8 secretion of Ker-CT cells overexpressing KRT32 wildtype and mutations with and without TNF treatment. P Analysis of luciferase activity after transfection with expression vectors encoding KRT32 wildtype and the variants (6 rare (MAF < 0.0005) and predicted to be damaging variants, 3 rare (MAF < 0.0005) and predicted to be benign variants, and 12 common (MAF > 0.0005) variants) in stimulated with TNF (20 ng/mL) for 12 h. Data are shown as means ± SEM of three independent biological repeats in ( C , E – G , J – L and N – P ). The P value was calculated using ordinary one-way ANOVA with Dunnett’s multiple comparisons test in ( C , P ); a two-sided unpaired Student’s t test in ( E – G , J – L ); and two-ANOVA with Dunnett’s multiple comparisons test in ( N ) and ( O ). Floating bars show the minimum, average, and maximum values within each group in ( P ). One representative experiment from two independent experiments with similar results is shown in ( D , H , I and M ). Source data are provided as file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-50481-z'>39048559</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-primary-antibodies-fcm-testing-3.jpgAnti-TNF alpha Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-TNF alpha antibody (PA1079). <br>
Overlay histogram showing CACO-2 cells stained with PA1079 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF alpha Antibody (PA1079, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-13287_2022_2980_fig7_html.pngAnti-TNF alpha Antibody Picoband®PF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-022-02980-3'>35941707</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-alpha-primary-antibodies-wb-testing-1.pngAnti-TNF alpha Antibody Picoband®Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1-5: mouse lung cancer tissue. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (PA1079) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. The expected band size for TNF alpha is at 26 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-primary-antibodies-wb-review-1.pngAnti-TNF alpha Antibody Picoband® Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: control group-normal mouse hippocampal tissue lysates,<br>
Lane 2: hippocampal tissue from Alzheimer’s disease model mouse,<br>
Lane 3: hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for TNF alpha at approximately 26 kDa. The expected band size for TNF alpha is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1079-tnf-primary-antibodies-wb-review-2.pngAnti-TNF alpha Antibody Picoband®Western blot analysis of TNF alpha using anti-TNF alpha antibody (PA1079). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: normal rat alveolar bone tissue lysates,<br>
Lane 2: alveolar bone from a diabetic bone defect model treated with EGCG,<br>
Lane 3: alveolar bone from a diabetic bone defect model treated with PGC-1α,<br>
Lane 4: alveolar bone from a diabetic bone defect model.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PA1079) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for TNF alpha at approximately 26 kDa. The expected band size for TNF alpha is at 26 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-tcf7-picoband-trade-antibody-a01315-2-boster.html2026-03-24T05:20:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01315-2-tcf7-primary-antibodies-wb-testing-1.jpgAnti-TCF7 Antibody Picoband® Western blot analysis of TCF7 using anti-TCF7 antibody (A01315-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human MOLT-4 whole cell lysates, <br>
Lane 3: mouse spleen tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7 antigen affinity purified polyclonal antibody (Catalog # A01315-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCF7 at approximately 50 kDa. The expected band size for TCF7 is at 42 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-hsd17b1-picoband-trade-antibody-a02198-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02198-HSD17B1-primary-antibodies-WB-testing-1.jpgAnti-HSD17B1 Antibody Picoband® Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (A02198).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: Human Placenta tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (Catalog # A02198) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B1 at approximately 40KD. The expected band size for HSD17B1 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02198-hsd17b1-primary-antibodies-if-testing-2.jpgAnti-HSD17B1 Antibody Picoband® IF analysis of HSD17B1 using anti-HSD17B1 antibody (A02198). <br>
HSD17B1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HSD17B1 Antibody (A02198) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02198-hsd17b1-primary-antibodies-ihc-testing-3.jpgAnti-HSD17B1 Antibody Picoband® IHC analysis of HSD17B1 using anti-HSD17B1 antibody (A02198). <br>
HSD17B1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B1 Antibody (A02198) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-akr1d1-picoband-trade-antibody-a05278-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-1-akr1d1-primary-antibodies-wb-testing-1.jpgAnti-AKR1D1 Antibody Picoband® Western blot analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: rat liver tissue lysates,<br>
Lane 4: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1D1 antigen affinity purified polyclonal antibody (Catalog # A05278-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1D1 at approximately 37 kDa. The expected band size for AKR1D1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-1-akr1d1-primary-antibodies-fcm-testing-2.jpgAnti-AKR1D1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-AKR1D1 antibody (A05278-1). <br>
Overlay histogram showing HepG2 cells stained with A05278-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1D1 Antibody (A05278-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-app-c99-picoband-trade-antibody-a00081-3-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00081-3-C99-primary-antibodies-WB-testing-1.jpgAnti-APP/C99 Antibody Picoband® Western blot analysis of APP/C99 using anti-APP/C99 antibody (A00081-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human T-47D whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP/C99 antigen affinity purified polyclonal antibody (Catalog # A00081-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP/C99 at approximately 99KD. The expected band size for APP/C99 is at 87KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00081-3-C99-primary-antibodies-IHC-testing-2.jpgAnti-APP/C99 Antibody Picoband® IHC analysis of C99 using anti-C99 antibody (A00081-3).<br>C99 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C99 Antibody (A00081-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00081-3-C99-primary-antibodies-IHC-testing-3.jpgAnti-APP/C99 Antibody Picoband® IHC analysis of C99 using anti-C99 antibody (A00081-3).<br>C99 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C99 Antibody (A00081-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00081-3-C99-primary-antibodies-IHC-testing-4.jpgAnti-APP/C99 Antibody Picoband® IHC analysis of C99 using anti-C99 antibody (A00081-3).
<br>C99 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C99 Antibody (A00081-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-aprt-picoband-trade-antibody-a02721-3-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02721-3-aprt-primary-antibodies-wb-testing-1_1.jpgAnti-Aprt Antibody Picoband® Western blot analysis of Aprt using anti-Aprt antibody (A02721-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: mouse liver tissue lysates,<br>
Lane 4: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aprt antigen affinity purified polyclonal antibody (Catalog # A02721-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aprt at approximately 20 kDa. The expected band size for Aprt is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02721-3-APRT-primary-antibodies-IHC-testing-2.jpgAnti-Aprt Antibody Picoband® IHC analysis of APRT using anti-APRT antibody (A02721-3).<br>APRT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APRT Antibody (A02721-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02721-3-aprt-primary-antibodies-wb-testing-2.jpgAnti-Aprt Antibody Picoband® Western blot analysis of Aprt using anti-Aprt antibody (A02721-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human SiHa whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aprt antigen affinity purified polyclonal antibody (Catalog # A02721-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aprt at approximately 20 kDa. The expected band size for Aprt is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02721-3-APRT-primary-antibodies-IHC-testing-3.jpgAnti-Aprt Antibody Picoband® IHC analysis of APRT using anti-APRT antibody (A02721-3).<br>APRT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APRT Antibody (A02721-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02721-3-APRT-primary-antibodies-IHC-testing-4.jpgAnti-Aprt Antibody Picoband® IHC analysis of APRT using anti-APRT antibody (A02721-3).<br>APRT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APRT Antibody (A02721-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02721-3-APRT-primary-antibodies-IHC-testing-5.jpgAnti-Aprt Antibody Picoband® IHC analysis of APRT using anti-APRT antibody (A02721-3).<br>APRT was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APRT Antibody (A02721-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02721-3-APRT-primary-antibodies-IHC-testing-6.jpgAnti-Aprt Antibody Picoband® IHC analysis of APRT using anti-APRT antibody (A02721-3).<br>APRT was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APRT Antibody (A02721-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-bat3-bag6-picoband-trade-antibody-a00967-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-wb-testing-1.jpgAnti-BAT3/BAG6 Antibody Picoband® Western blot analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAT3/BAG6 antigen affinity purified polyclonal antibody (Catalog # A00967-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAT3/BAG6 at approximately 150 kDa. The expected band size for BAT3/BAG6 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-ihc-testing-2.jpgAnti-BAT3/BAG6 Antibody Picoband® IHC analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1). <br>
BAT3/BAG6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAT3/BAG6 Antibody (A00967-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-ihc-testing-3_1.jpgAnti-BAT3/BAG6 Antibody Picoband® IHC analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1). <br>
BAT3/BAG6 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAT3/BAG6 Antibody (A00967-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-if-testing-4.jpgAnti-BAT3/BAG6 Antibody Picoband® IF analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1) and anti-Beta Tubulin antibody (M01857-3).<br>
BAT3/BAG6 was detected in immunocytochemical section of PC3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BAT3/BAG6 Antibody (A00967-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-fcm-testing-5.jpgAnti-BAT3/BAG6 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-BAT3/BAG6 antibody (A00967-1). <br>
Overlay histogram showing SH-SY5Y cells stained with A00967-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAT3/BAG6 Antibody (A00967-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00967-1-bag6-primary-antibodies-wb-testing-6.jpgAnti-BAT3/BAG6 Antibody Picoband® Western blot analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody (A00967-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: human U87 whole cell lysates,<br>
Lane 4: human SH-SY5Y whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAT3/BAG6 antigen affinity purified polyclonal antibody (Catalog # A00967-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-FITC secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for BAT3/BAG6 at approximately 150 kDa. The expected band size for BAT3/BAG6 is at 119 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-bmp15-picoband-trade-antibody-a01842-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01842-bmp15-primary-antibodies-ihc-testing-10.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842). <br>BMP15 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01842-bmp15-primary-antibodies-wb-testing-1.jpgAnti-BMP15 Antibody Picoband® Western blot analysis of BMP15 using anti-BMP15 antibody (A01842). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human Caco-2 whole cell lysates,<br>Lane 4: rat lung tissue lysates,<br>Lane 5: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP15 antigen affinity purified polyclonal antibody (Catalog # A01842) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP15 at approximately 45KD. The expected band size for BMP15 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-2.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-3.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-4.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-5.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-6.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-7.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-8.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01842-BMP15-primary-antibodies-IHC-testing-9.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842).<br>BMP15 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01842-bmp15-primary-antibodies-ihc-testing-11.jpgAnti-BMP15 Antibody Picoband® IHC analysis of BMP15 using anti-BMP15 antibody (A01842). <br>BMP15 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BMP15 Antibody (A01842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-gpr2-ccr10-picoband-trade-antibody-a04731-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04731-1-CCR10-primary-antibodies-WB-testing-1.jpgAnti-GPR2/CCR10 Antibody Picoband® Western blot analysis of CCR10 using anti-CCR10 antibody (A04731-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human K562 whole cell lysates,<br>Lane 3: human PC-3 whole cell lysates,<br>Lane 4: human A549 whole cell lysates,<br>Lane 5: human T-47D whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR10 antigen affinity purified polyclonal antibody (Catalog # A04731-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR10 at approximately 45KD. The expected band size for CCR10 is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04731-1-CCR10-primary-antibodies-IHC-testing-2.jpgAnti-GPR2/CCR10 Antibody Picoband® IHC analysis of CCR10 using anti-CCR10 antibody (A04731-1).<br>CCR10 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR10 Antibody (A04731-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04731-1-CCR10-primary-antibodies-IHC-testing-3.jpgAnti-GPR2/CCR10 Antibody Picoband® IHC analysis of CCR10 using anti-CCR10 antibody (A04731-1).<br>CCR10 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR10 Antibody (A04731-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04731-1-CCR10-primary-antibodies-IHC-testing-4.jpgAnti-GPR2/CCR10 Antibody Picoband® IHC analysis of CCR10 using anti-CCR10 antibody (A04731-1).
<br>CCR10 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR10 Antibody (A04731-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>
https://www.bosterbio.com/products/primary-antibodies/anti-cda-picoband-trade-antibody-a01578-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-WB-testing-1.jpgAnti-CDA Antibody Picoband® Western blot analysis of CDA using anti-CDA antibody (A01578-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDA antigen affinity purified polyclonal antibody (Catalog # A01578-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDA at approximately 16KD. The expected band size for CDA is at 16KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-2.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-3.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-4.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-5.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-6.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-7.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01578-1-CDA-primary-antibodies-IHC-testing-8.jpgAnti-CDA Antibody Picoband® IHC analysis of CDA using anti-CDA antibody (A01578-1).<br>CDA was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDA Antibody (A01578-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cdk8-picoband-trade-antibody-a01493-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01493-1-cdk8-primary-antibodies-wb-testing-1_1.jpgAnti-CDK8 Antibody Picoband® Western blot analysis of CDK8 using anti-CDK8 antibody (A01493-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: human HEK293 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK8 antigen affinity purified polyclonal antibody (Catalog # A01493-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK8 at approximately 53 kDa. The expected band size for CDK8 is at 53 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-chm-picoband-trade-antibody-a00814-2-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-fcm-testing-6.jpgAnti-CHM Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CHM antibody (A00814-2). <br>Overlay histogram showing U937 cells stained with A00814-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHM Antibody (A00814-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-wb-testing-1_1.jpgAnti-CHM Antibody Picoband® Western blot analysis of CHM using anti-CHM antibody (A00814-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: human 293T whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human CACO-2 whole cell lysates, <br>
Lane 6: human SiHa whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHM antigen affinity purified polyclonal antibody (Catalog # A00814-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHM at approximately 100 kDa. The expected band size for CHM is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-ihc-testing-2.jpgAnti-CHM Antibody Picoband® IHC analysis of CHM using anti-CHM antibody (A00814-2). <br>
CHM was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHM Antibody (A00814-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-ihc-testing-3.jpgAnti-CHM Antibody Picoband® IHC analysis of CHM using anti-CHM antibody (A00814-2). <br>
CHM was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHM Antibody (A00814-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-ihc-testing-4.jpgAnti-CHM Antibody Picoband® IHC analysis of CHM using anti-CHM antibody (A00814-2). <br>
CHM was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHM Antibody (A00814-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00814-2-chm-primary-antibodies-if-testing-5.jpgAnti-CHM Antibody Picoband® IF analysis of CHM using anti-CHM antibody (A00814-2). <br>
CHM was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CHM Antibody (A00814-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-collagen-xvii-col17a1-picoband-trade-antibody-a03031-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03031-1-col17a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen XVII/COL17A1 Antibody Picoband® Western blot analysis of COL17A1 using anti-COL17A1 antibody (A03031-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 antigen affinity purified polyclonal antibody (Catalog # A03031-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL17A1 at approximately 125KD. The expected band size for COL17A1 is at 150KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dok7-picoband-trade-antibody-a05165-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05165-1-dok7-primary-antibodies-wb-testing-1.jpgAnti-DOK7 Antibody Picoband® Western blot analysis of DOK7 using anti-DOK7 antibody (A05165-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOK7 antigen affinity purified polyclonal antibody (Catalog # A05165-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOK7 at approximately 53 kDa. The expected band size for DOK7 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05165-1-dok7-primary-antibodies-wb-testing-2.jpgAnti-DOK7 Antibody Picoband® Western blot analysis of DOK7 using anti-DOK7 antibody (A05165-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat skeletal muscle tissue lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: rat heart tissue lysates,<br>
Lane 4: rat L6 whole cell lysates,<br>
Lane 5: mouse skeletal muscle tissue lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse C2C12 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOK7 antigen affinity purified polyclonal antibody (Catalog # A05165-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOK7 at approximately 53 kDa. The expected band size for DOK7 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05165-1-dok7-primary-antibodies-ihc-testing-3_1.jpgAnti-DOK7 Antibody Picoband® IHC analysis of DOK7 using anti-DOK7 antibody (A05165-1). <br>
DOK7 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOK7 Antibody (A05165-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05165-1-dok7-primary-antibodies-ihc-testing-4_1.jpgAnti-DOK7 Antibody Picoband® IHC analysis of DOK7 using anti-DOK7 antibody (A05165-1). <br>
DOK7 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOK7 Antibody (A05165-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05165-1-dok7-primary-antibodies-fcm-testing-5.jpgAnti-DOK7 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-DOK7 antibody (A05165-1). <br>
Overlay histogram showing RT4 cells stained with A05165-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DOK7 Antibody (A05165-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tmem166-eva1a-picoband-trade-antibody-a11580-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11580-1-EVA1A-primary-antibodies-WB-testing-1.jpgAnti-TMEM166/EVA1A Antibody Picoband® Western blot analysis of EVA1A using anti-EVA1A antibody (A11580-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates,<br>Lane 2: human PC-3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVA1A antigen affinity purified polyclonal antibody (Catalog # A11580-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVA1A at approximately 21KD. The expected band size for EVA1A is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11580-1-EVA1A-primary-antibodies-IHC-testing-2.jpgAnti-TMEM166/EVA1A Antibody Picoband® IHC analysis of EVA1A using anti-EVA1A antibody (A11580-1).<br>EVA1A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EVA1A Antibody (A11580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11580-1-EVA1A-primary-antibodies-IHC-testing-3.jpgAnti-TMEM166/EVA1A Antibody Picoband® IHC analysis of EVA1A using anti-EVA1A antibody (A11580-1).<br>EVA1A was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EVA1A Antibody (A11580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11580-1-eva1a-primary-antibodies-fc-testing-4.pngAnti-TMEM166/EVA1A Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-EVA1A antibody (A11580-1). <br>Overlay histogram showing A549 cells stained with A11580-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EVA1A Antibody (A11580-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-hectd3-picoband-trade-antibody-a11560-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11560-HECTD3-primary-antibodies-WB-testing-1.jpgAnti-HECTD3 Antibody Picoband® Western blot analysis of HECTD3 using anti-HECTD3 antibody (A11560). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates,<br>Lane 2: rat brain tissue lysates,<br>Lane 3: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HECTD3 antigen affinity purified polyclonal antibody (Catalog # A11560) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HECTD3 at approximately 97KD. The expected band size for HECTD3 is at 97KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11560-HECTD3-primary-antibodies-IHC-testing-2.jpgAnti-HECTD3 Antibody Picoband® IHC analysis of HECTD3 using anti-HECTD3 antibody (A11560).<br>HECTD3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HECTD3 Antibody (A11560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11560-HECTD3-primary-antibodies-IHC-testing-3.jpgAnti-HECTD3 Antibody Picoband® IHC analysis of HECTD3 using anti-HECTD3 antibody (A11560).<br>HECTD3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HECTD3 Antibody (A11560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11560-HECTD3-primary-antibodies-IHC-testing-4.jpgAnti-HECTD3 Antibody Picoband® IHC analysis of HECTD3 using anti-HECTD3 antibody (A11560).
<br>HECTD3 was detected in paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HECTD3 Antibody (A11560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11560-hectd3-primary-antibodies-fc-testing-5.pngAnti-HECTD3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-HECTD3 antibody (A11560). <br>Overlay histogram showing A549 cells stained with A11560 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HECTD3 Antibody (A11560,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-igll1-picoband-trade-antibody-a08965-1-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-WB-testing-1.jpgAnti-IGLL1 Antibody Picoband® Western blot analysis of IGLL1 using anti-IGLL1 antibody (A08965-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGLL1 antigen affinity purified polyclonal antibody (Catalog # A08965-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGLL1 at approximately 26KD. The expected band size for IGLL1 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-IHC-testing-2.jpgAnti-IGLL1 Antibody Picoband® IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).<br>IGLL1 was detected in paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGLL1 Antibody (A08965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-IHC-testing-3.jpgAnti-IGLL1 Antibody Picoband® IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).<br>IGLL1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGLL1 Antibody (A08965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-IHC-testing-4.jpgAnti-IGLL1 Antibody Picoband® IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).<br>IGLL1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGLL1 Antibody (A08965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-IHC-testing-5.jpgAnti-IGLL1 Antibody Picoband® IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).<br>IGLL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGLL1 Antibody (A08965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08965-1-IGLL1-primary-antibodies-IHC-testing-6.jpgAnti-IGLL1 Antibody Picoband® IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).<br>IGLL1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGLL1 Antibody (A08965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-il34-picoband-trade-antibody-a06903-2-boster.html2026-03-24T05:20:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06903-2-il34-primary-antibodies-wb-testing-1_1.jpgAnti-IL34 Antibody Picoband® Western blot analysis of IL34 using anti-IL34 antibody (A06903-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whle cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human U87 whole cell lysates,<br>
Lane 5: human CACO-2 whole cell lysates,<br>
Lane 6: human U20S whole cell lysates,<br>
Lane 7: human HEK293 whole cell lysates,<br>
Lane 8: rat C6 whole cell lysates,<br>
Lane 9: mouse ANA-1 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL34 antigen affinity purified polyclonal antibody (Catalog # A06903-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL34 at approximately 39 kDa. The expected band size for IL34 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06903-2-il34-primary-antibodies-ihc-testing-2.jpgAnti-IL34 Antibody Picoband® IHC analysis of IL34 using anti-IL34 antibody (A06903-2). <br>
IL34 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL34 Antibody (A06903-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-iqgap1-picoband-trade-antibody-a01603-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01603-IQGAP1-primary-antibodies-WB-testing-1.jpgAnti-IQGAP1 Antibody Picoband® Western blot analysis of IQGAP1 using anti-IQGAP1 antibody (A01603). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human U-87MG whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP1 antigen affinity purified polyclonal antibody (Catalog # A01603) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP1 at approximately 189KD. The expected band size for IQGAP1 is at 189KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01603-IQGAP1-primary-antibodies-IHC-testing-2.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603).<br>IQGAP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IQGAP1 Antibody (A01603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01603-IQGAP1-primary-antibodies-IHC-testing-3.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603).<br>IQGAP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IQGAP1 Antibody (A01603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01603-IQGAP1-primary-antibodies-IHC-testing-4.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603).<br>IQGAP1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IQGAP1 Antibody (A01603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01603-IQGAP1-primary-antibodies-IHC-testing-5.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603).<br>IQGAP1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IQGAP1 Antibody (A01603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-iqgap1-primary-antibodies-if-testing-6.jpgAnti-IQGAP1 Antibody Picoband® IF analysis of IQGAP1 using anti-IQGAP1 antibody (A01603). <br>
IQGAP1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IQGAP1 Antibody (A01603) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-iqgap1-primary-antibodies-fcm-testing-7.jpgAnti-IQGAP1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti- IQGAP1 antibody (A01603). <br>Overlay histogram showing HepG2 cells stained with A01603 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti- IQGAP1 Antibody (A01603, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kbtbd2-picoband-trade-antibody-a15277-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15277-1-kbtbd2-primary-antibodies-wb-testing-1_1.jpgAnti-KBTBD2 Antibody Picoband® Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human U20S whole cell lysates,<br>
Lane 4: human COLO320 whole cell lysates,<br>
Lane 5: human SW620 whole cell lysates,<br>
Lane 6: human Caco-2 whole cell lysates,<br>
Lane 7: human placenta tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KBTBD2 antigen affinity purified polyclonal antibody (Catalog # A15277-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KBTBD2 at approximately 72 kDa. The expected band size for KBTBD2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15277-1-kbtbd2-primary-antibodies-wb-testing-2.jpgAnti-KBTBD2 Antibody Picoband® Western blot analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat heart tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: rat PC-12 whole cell lysates,<br>
Lane 4: mouse heart tissue lysates,<br>
Lane 5: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KBTBD2 antigen affinity purified polyclonal antibody (Catalog # A15277-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KBTBD2 at approximately 72 kDa. The expected band size for KBTBD2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15277-1-kbtbd2-primary-antibodies-fcm-testing-4.jpgAnti-KBTBD2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-KBTBD2 antibody (A15277-1). <br>Overlay histogram showing U87 cells stained with A15277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KBTBD2 Antibody (A15277-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15277-1-kbtbd2-primary-antibodies-if-testing-3.jpgAnti-KBTBD2 Antibody Picoband® IF analysis of KBTBD2 using anti-KBTBD2 antibody (A15277-1).<br> KBTBD2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KBTBD2 Antibody (A15277-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-leucyl-trna-synthetase-lars-picoband-trade-antibody-a01109-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01109-lars-primary-antibodies-wb-testing-1.jpgAnti-Leucyl tRNA synthetase /LARS Antibody Picoband® Western blot analysis of LARS using anti-LARS antibody (A01109). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HL-60 whole cell lysates,<br>Lane 2: human K562 whole cell lysates,<br>Lane 3: human THP-1 whole cell lysates,<br>Lane 4: human Caco-2 whole cell lysates,<br>Lane 5: rat liver tissue lysates,<br>Lane 6: mouse brain tissue lysates,<br>Lane 7: mouse liver tissue lysates,<br>Lane 8: mouse NIH3T3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARS antigen affinity purified polyclonal antibody (Catalog # A01109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARS at approximately 134KD. The expected band size for LARS is at 134KD.
https://www.bosterbio.com/products/primary-antibodies/anti-plastin-l-lcp1-picoband-trade-antibody-a03361-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03361-lcp1-primary-antibodies-wb-testing-1.jpgAnti-Plastin L/LCP1 Antibody Picoband® Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates,<br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: mouse spleen tissue lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03361-LCP1-primary-antibodies-IHC-testing-2.jpgAnti-Plastin L/LCP1 Antibody Picoband® IHC analysis of LCP1 using anti-LCP1 antibody (A03361).<br>LCP1 was detected in paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LCP1 Antibody (A03361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03361-LCP1-primary-antibodies-IHC-testing-3.jpgAnti-Plastin L/LCP1 Antibody Picoband® IHC analysis of LCP1 using anti-LCP1 antibody (A03361).<br>LCP1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LCP1 Antibody (A03361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03361-LCP1-primary-antibodies-IHC-testing-4.jpgAnti-Plastin L/LCP1 Antibody Picoband® IHC analysis of LCP1 using anti-LCP1 antibody (A03361).
<br>LCP1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LCP1 Antibody (A03361) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03361-lcp1-primary-antibodies-fc-testing-5.pngAnti-Plastin L/LCP1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-LCP1 antibody (A03361).<br>Overlay histogram showing U20S cells stained with A03361 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LCP1 Antibody (A03361,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03361-lcp1-primary-antibodies-if-testing-6.jpgAnti-Plastin L/LCP1 Antibody Picoband® IF analysis of LCP1 using anti-LCP1 antibody (A03361). <br>
LCP1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LCP1 Antibody (A03361) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-mcur1-picoband-trade-antibody-a08547-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-WB-testing-1.jpgAnti-MCUR1 Antibody Picoband® Western blot analysis of MCUR1 using anti-MCUR1 antibody (A08547-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates.<br>Lane 2: human MDA-MB-231 whole cell lysates,<br>Lane 3: human HL-60 whole cell lysates,<br>Lane 4: human MDA-MB-453 whole cell lysates,<br>Lane 5: human A431 whole cell lysates,<br>Lane 6: human Caco-2 whole cell lysates,<br>Lane 7: rat spleen tissue lysates,<br>Lane 8: mouse lung tissue lysates,<br>Lane 9: mouse Ana-1 whole cell lysates, <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCUR1 antigen affinity purified polyclonal antibody (Catalog # A08547-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCUR1 at approximately 40KD. The expected band size for MCUR1 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-IHC-testing-2.jpgAnti-MCUR1 Antibody Picoband® IHC analysis of MCUR1 using anti-MCUR1 antibody (A08547-1).<br>MCUR1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCUR1 Antibody (A08547-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-IHC-testing-3.jpgAnti-MCUR1 Antibody Picoband® IHC analysis of MCUR1 using anti-MCUR1 antibody (A08547-1).<br>MCUR1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCUR1 Antibody (A08547-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-IHC-testing-4.jpgAnti-MCUR1 Antibody Picoband® IHC analysis of MCUR1 using anti-MCUR1 antibody (A08547-1).<br>MCUR1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCUR1 Antibody (A08547-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-IHC-testing-5.jpgAnti-MCUR1 Antibody Picoband® IHC analysis of MCUR1 using anti-MCUR1 antibody (A08547-1).<br>MCUR1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCUR1 Antibody (A08547-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08547-1-MCUR1-primary-antibodies-IHC-testing-6.jpgAnti-MCUR1 Antibody Picoband® IHC analysis of MCUR1 using anti-MCUR1 antibody (A08547-1).<br>MCUR1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCUR1 Antibody (A08547-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tpa-tissue-plasminogen-activator-plat-picoband-trade-antibody-a02965-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02965-1-plat-primary-antibodies-wb-testing-1.jpgAnti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human U2OS whole cell lysates,<br>Lane 3: human U-87MG whole cell lysates,<br>Lane 4: rat liver tissue lysates,<br>Lane 5: rat kidney tissue lysates,<br>Lane 6: mouse liver tissue lysates,<br>Lane 7: mouse kidney tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 63-69KD. The expected band size for PLAT is at 63KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02965-1-PLAT-primary-antibodies-IHC-testing-2.jpgAnti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® IHC analysis of PLAT using anti-PLAT antibody (A02965-1).<br>PLAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLAT Antibody (A02965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02965-1-plat-primary-antibodies-fc-testing-3.pngAnti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PLAT antibody (A02965-1). <br>Overlay histogram showing U251 cells stained with A02965-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAT Antibody (A02965-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-ppcs-picoband-trade-antibody-a05567-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-ppcs-primary-antibodies-wb-testing-1_1.jpgAnti-PPCS Antibody Picoband® Western blot analysis of PPCS using anti-PPCS antibody (A05567-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: human PC-3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPCS antigen affinity purified polyclonal antibody (Catalog # A05567-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPCS at approximately 35 kDa. The expected band size for PPCS is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05567-1-PPCS-primary-antibodies-IHC-testing-2.jpgAnti-PPCS Antibody Picoband® IHC analysis of PPCS using anti-PPCS antibody (A05567-1).<br>PPCS was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPCS Antibody (A05567-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-ppcs-primary-antibodies-fc-testing-3.pngAnti-PPCS Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PPCS antibody (A05567-1). Overlay histogram showing A431 cells stained with A05567-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPCS Antibody (A05567-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-ppcs-primary-antibodies-if-testing-4.jpg.jpgAnti-PPCS Antibody Picoband® IF analysis of PPCS using anti-PPCS antibody (A05567-1). <br> PPCS was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PPCS Antibody (A05567-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-ppcs-primary-antibodies-if-testing-5.jpg.jpgAnti-PPCS Antibody Picoband® IF analysis of PPCS using anti-PPCS antibody (A05567-1). <br> PPCS was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PPCS Antibody (A05567-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-6.jpgAnti-PPCS Antibody Picoband® IHC analysis of PPCS using anti-PPCS antibody (A05567-1). <br> PPCS was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPCS Antibody (A05567-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05567-1-7.jpgAnti-PPCS Antibody Picoband® IHC analysis of PPCS using anti-PPCS antibody (A05567-1). <br> PPCS was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPCS Antibody (A05567-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-rab11b-picoband-trade-antibody-a04526-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-WB-testing-1.jpgAnti-RAB11B Antibody Picoband® Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: rat ovary tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: mouse lung tissue lysates,<br>Lane 6: mouse ovary tissue lysates,<br>Lane 7: mouse kidney tissue lysates,<br>Lane 8: mouse SP20 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # A04526-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-WB-testing-2.jpgAnti-RAB11B Antibody Picoband® Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human A549 whole cell lysates,<br>Lane 3: human U-87MG whole cell lysates,<br>Lane 4: human PC-3 whole cell lysates,<br>Lane 5: human Hela whole cell lysates,<br>Lane 6: human Caco-2 whole cell lysates,<br>Lane 7: human HL-60 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # A04526-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-IHC-testing-3.jpgAnti-RAB11B Antibody Picoband® IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).<br>RAB11B was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB11B Antibody (A04526-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-IHC-testing-4.jpgAnti-RAB11B Antibody Picoband® IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).<br>RAB11B was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB11B Antibody (A04526-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-IHC-testing-5.jpgAnti-RAB11B Antibody Picoband® IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).<br>RAB11B was detected in paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB11B Antibody (A04526-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04526-1-RAB11B-primary-antibodies-IHC-testing-6.jpgAnti-RAB11B Antibody Picoband® IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).<br>RAB11B was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB11B Antibody (A04526-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04526-1-rab11b-primary-antibodies-fc-testing-7.pngAnti-RAB11B Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-RAB11B antibody (A04526-1). <br> Overlay histogram showing A549 cells stained with A04526-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB11B Antibody (A04526-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04526-1-rab11b-primary-antibodies-if-testing-8.jpgAnti-RAB11B Antibody Picoband® IF analysis of RAB11B using anti-RAB11B antibody (A04526-1). <br> RAB11B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RAB11B Antibody (A04526-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-rhob-picoband-trade-antibody-a01550-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01550-1-rhob-primary-antibodies-wb-testing-1.jpgAnti-RHOB Antibody Picoband® Western blot analysis of RHOB using anti-RHOB antibody (A01550-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: rat C6 whole cell lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: monkey COS-7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOB antigen affinity purified polyclonal antibody (Catalog # A01550-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHOB at approximately 22 kDa. The expected band size for RHOB is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01550-1-RHOB-primary-antibodies-IHC-testing-2.jpgAnti-RHOB Antibody Picoband® IHC analysis of RHOB using anti-RHOB antibody (A01550-1).<br>RHOB was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RHOB Antibody (A01550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01550-1-RHOB-primary-antibodies-IHC-testing-3.jpgAnti-RHOB Antibody Picoband® IHC analysis of RHOB using anti-RHOB antibody (A01550-1).<br>RHOB was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RHOB Antibody (A01550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01550-1-rhob-primary-antibodies-if-testing-4.jpgAnti-RHOB Antibody Picoband® IF analysis of RHOB using anti-RHOB antibody (A01550-1). <br>
RHOB was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RHOB Antibody (A01550-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-rmi2-picoband-trade-antibody-a08685-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08685-rmi2-primary-antibodies-wb-testing-1.jpgAnti-RMI2 Antibody Picoband® Western blot analysis of RMI2 using anti-RMI2 antibody (A08685). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human T-47D whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat thymus tissue lysates,<br> Lane 6: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RMI2 antigen affinity purified polyclonal antibody (Catalog # A08685) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RMI2 at approximately 19KD. The expected band size for RMI2 is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08685-RMI2-primary-antibodies-IHC-testing-2.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685).<br>RMI2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08685-RMI2-primary-antibodies-IHC-testing-3.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685).<br>RMI2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08685-RMI2-primary-antibodies-IHC-testing-4.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685).<br>RMI2 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08685-RMI2-primary-antibodies-IHC-testing-5.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685).<br>RMI2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08685-RMI2-primary-antibodies-IHC-testing-6.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685).<br>RMI2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08685-rmi2-primary-antibodies-fc-testing-7.jpg.pngAnti-RMI2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RMI2 antibody (A08685). <br> Overlay histogram showing U20S cells stained with A08685 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RMI2 Antibody (A08685,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08685-8.jpgAnti-RMI2 Antibody Picoband® IHC analysis of RMI2 using anti-RMI2 antibody (A08685). <br> RMI2 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RMI2 Antibody (A08685) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-rrbp1-picoband-trade-antibody-a07074-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07074-1-RRBP1-primary-antibodies-WB-testing-1.jpgAnti-RRBP1 Antibody Picoband® Western blot analysis of RRBP1 using anti-RRBP1 antibody (A07074-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates,<br>Lane 2: human HepG2 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRBP1 antigen affinity purified polyclonal antibody (Catalog # A07074-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRBP1 at approximately 200KD. The expected band size for RRBP1 is at 152KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07074-1-RRBP1-primary-antibodies-IHC-testing-2.jpgAnti-RRBP1 Antibody Picoband® IHC analysis of RRBP1 using anti-RRBP1 antibody (A07074-1).<br>RRBP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RRBP1 Antibody (A07074-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07074-1-RRBP1-primary-antibodies-IHC-testing-3.jpgAnti-RRBP1 Antibody Picoband® IHC analysis of RRBP1 using anti-RRBP1 antibody (A07074-1).<br>RRBP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RRBP1 Antibody (A07074-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07074-1-RRBP1-primary-antibodies-IHC-testing-4.jpgAnti-RRBP1 Antibody Picoband® IHC analysis of RRBP1 using anti-RRBP1 antibody (A07074-1).<br>RRBP1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RRBP1 Antibody (A07074-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07074-1-RRBP1-primary-antibodies-IHC-testing-5.jpgAnti-RRBP1 Antibody Picoband® IHC analysis of RRBP1 using anti-RRBP1 antibody (A07074-1).<br>RRBP1 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RRBP1 Antibody (A07074-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07074-1-rrbp1-primary-antibodies-fc-testing-6.pngAnti-RRBP1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-RRBP1 antibody (A07074-1). <br> Overlay histogram showing A549 cells stained with A07074-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRBP1 Antibody (A07074-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07074-1-rrbp1-primary-antibodies-fc-testing-7.pngAnti-RRBP1 Antibody Picoband® Flow Cytometry analysis of SIHA cells using anti-RRBP1 antibody (A07074-1). <br> Overlay histogram showing SIHA cells stained with A07074-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRBP1 Antibody (A07074-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07074-1-rrbp1-primary-antibodies-if-testing-8.jpg.jpgAnti-RRBP1 Antibody Picoband® IF analysis of RRBP1 using anti-RRBP1 antibody (A07074-1). <br> RRBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RRBP1 Antibody (A07074-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-sfrp1-picoband-trade-antibody-a01968-2-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01968-2-sfrp1-primary-antibodies-wb-testing-1.jpgAnti-SFRP1 Antibody Picoband® Western blot analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates,<br>Lane 2: human U2OS whole cell lysates,<br>Lane 3: human PC-3 whole cell lysates,<br>Lane 4: rat heart tissue lysates,<br>Lane 5: mouse heart tissue lysates,<br>Lane 6: mouse HEPA1-6 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 antigen affinity purified polyclonal antibody (Catalog # A01968-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP1 at approximately 35KD. The expected band size for SFRP1 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-2.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).<br>SFRP1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-3.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).<br>SFRP1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-4.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).<br>SFRP1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-5.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).<br>SFRP1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-6.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).<br>SFRP1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01968-2-SFRP1-primary-antibodies-IHC-testing-7.jpgAnti-SFRP1 Antibody Picoband® IHC analysis of SFRP1 using anti-SFRP1 antibody (A01968-2).
<br>SFRP1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01968-2-sfrp1-primary-antibodies-if-testing-8.jpgAnti-SFRP1 Antibody Picoband® IF analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). <br>
SFRP1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SFRP1 Antibody (A01968-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01968-2-sfrp1-primary-antibodies-fcm-testing-9.jpgAnti-SFRP1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SFRP1 antibody (A01968-2).<br>Overlay histogram showing A549 cells stained with A01968-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP1 Antibody (A01968-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-timm17a-picoband-trade-antibody-a12168-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-WB-testing-1.jpgAnti-TIMM17A Antibody Picoband® Western blot analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: rat liver tissue lysates,<br>Lane 4: rat brain tissue lysates,<br>Lane 5: mouse heart tissue lysates,<br>Lane 6: mouse lung tissue lysates,<br>Lane 7: mouse liver tissue lysates,<br>Lane 8: mouse brain tissue lysates,<br>Lane 9: mouse Neuro-2a whole cell lysates, <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM17A antigen affinity purified polyclonal antibody (Catalog # A12168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMM17A at approximately 18KD. The expected band size for TIMM17A is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12168-1-timm17a-primary-antibodies-if-testing-10.jpgAnti-TIMM17A Antibody Picoband® IF analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br> TIMM17A was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-WB-testing-2.jpgAnti-TIMM17A Antibody Picoband® Western blot analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human K562 whole cell lysates,<br>Lane 3: human T-47D whole cell lysates,<br>Lane 4: human Caco-2 whole cell lysates,<br>Lane 5: human Hela whole cell lysates,<br>Lane 6: human THP-1 whole cell lysates,<br>Lane 7: human A431 whole cell lysates,<br>Lane 8: human A549 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM17A antigen affinity purified polyclonal antibody (Catalog # A12168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMM17A at approximately 18KD. The expected band size for TIMM17A is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-IHC-testing-3.jpgAnti-TIMM17A Antibody Picoband® IHC analysis of TIMM17A using anti-TIMM17A antibody (A12168-1).<br>TIMM17A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-IHC-testing-4.jpgAnti-TIMM17A Antibody Picoband® IHC analysis of TIMM17A using anti-TIMM17A antibody (A12168-1).<br>TIMM17A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-IHC-testing-5.jpgAnti-TIMM17A Antibody Picoband® IHC analysis of TIMM17A using anti-TIMM17A antibody (A12168-1).<br>TIMM17A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12168-1-TIMM17A-primary-antibodies-IHC-testing-6.jpgAnti-TIMM17A Antibody Picoband® IHC analysis of TIMM17A using anti-TIMM17A antibody (A12168-1).<br>TIMM17A was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12168-1-timm17a-primary-antibodies-fc-testing-7.pngAnti-TIMM17A Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-TIMM17A antibody (A12168-1). <br>Overlay histogram showing A431 cells stained with A12168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM17A Antibody (A12168-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12168-1-timm17a-primary-antibodies-if-testing-8.jpgAnti-TIMM17A Antibody Picoband® IF analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br> TIMM17A was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12168-1-timm17a-primary-antibodies-if-testing-9.jpgAnti-TIMM17A Antibody Picoband® IF analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br> TIMM17A was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12168-1-11.jpgAnti-TIMM17A Antibody Picoband® IHC analysis of TIMM17A using anti-TIMM17A antibody (A12168-1). <br> TIMM17A was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TIMM17A Antibody (A12168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-wee1-picoband-trade-antibody-a01319-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01319-1-wee1-primary-antibodies-wb-testing-1.jpgAnti-WEE1 Antibody Picoband® Western blot analysis of WEE1 using anti-WEE1 antibody (A01319-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human A431 whole cell lysates,<br>Lane 4: human U2OS whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # A01319-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01319-1-wee1-primary-antibodies-ihc-testing-2.jpgAnti-WEE1 Antibody Picoband® IHC analysis of WEE1 using anti-WEE1 antibody (A01319-1). <br>
WEE1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WEE1 Antibody (A01319-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01319-1-wee1-primary-antibodies-if-testing-3.jpgAnti-WEE1 Antibody Picoband® IF analysis of WEE1 using anti-WEE1 antibody (A01319-1). <br>
WEE1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-WEE1 Antibody (A01319-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01319-1-wee1-primary-antibodies-fcm-testing-4.jpgAnti-WEE1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-WEE1 antibody (A01319-1).<br>Overlay histogram showing SiHa cells stained with A01319-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WEE1 Antibody (A01319-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-zp3-picoband-trade-antibody-a03543-1-boster.html2026-03-24T05:20:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03543-1-zp3-primary-antibodies-wb-testing-1_1.jpgAnti-ZP3 Antibody Picoband® Western blot analysis of ZP3 using anti-ZP3 antibody (A03543-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP3 antigen affinity purified polyclonal antibody (Catalog # A03543-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP3 at approximately 47 kDa. The expected band size for ZP3 is at 47 kDa.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shp2-ptpn11-picoband-trade-antibody-monoclonal-m00150-2-boster.html2026-04-01T05:01:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-wb-testing-1_1.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6)Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
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Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human RT4 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates.<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: rat heart tissue lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHP2/PTPN11 antigen affinity purified monoclonal antibody (Catalog # M00150-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 68 kDa. The expected band size for SHP2/PTPN11 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-2.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2). <br>
SHP2/PTPN11 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-3.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2). <br>
SHP2/PTPN11 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-4.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2). <br>
SHP2/PTPN11 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-5.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2). <br>
SHP2/PTPN11 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-if-testing-6.jpgAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) IF analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2). <br>
SHP2/PTPN11 was detected in immunocytochemical section of U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-2-ptpn11-primary-antibodies-fcm-testing-7.pngAnti-SHP2/PTPN11 Antibody Picoband® (monoclonal, 2E6) Flow Cytometry analysis of A549 cells using anti-SHP2/PTPN11 antibody (M00150-2). <br>Overlay histogram showing A549 cells stained with M00150-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wwox-picoband-trade-antibody-monoclonal-m01223-boster.html2026-03-24T05:21:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-wb-testing-1.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) Western blot analysis of WWOX using anti-WWOX antibody (M01223).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates;<br>
Lane 2: human T-47D whole cell lysates;<br>
Lane 3: human HepG2 whole cell lysates;<br>
Lane 4: human U-87MG whole cell lysates;<br>
Lane 5: human A549 whole cell lysates;<br>
Lane 6: human Hela whole cell lysates;<br>
Lane 7: human PC-3 whole cell lysates;<br>
Lane 8: human Caco-2 whole cell lysates;<br>
Lane 9: rat testicular tissue lysates;<br>
Lane 10: rat brain tissue lysates;<br>
Lane 11: rat liver tissue lysates;<br>
Lane 12: mouse testicular tissue lysates;<br>
Lane 13: mouse brain tissue lysates;<br>
Lane 14: mouse liver tissue lysates;<br>
Lane 15: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-WWOX antigen affinity purified monoclonal antibody (Catalog # M01223) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for WWOX at approximately 47KD. The expected band size for WWOX is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-ihc-testing-2.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) IHC analysis of WWOX using anti-WWOX antibody (M01223). <br>
WWOX was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-WWOX Antibody (M01223) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-ihc-testing-3.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) IHC analysis of WWOX using anti-WWOX antibody (M01223). <br>
WWOX was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-WWOX Antibody (M01223) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-ihc-testing-4.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) IHC analysis of WWOX using anti-WWOX antibody (M01223). <br>
WWOX was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-WWOX Antibody (M01223) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-ihc-testing-5.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) IHC analysis of WWOX using anti-WWOX antibody (M01223). <br>
WWOX was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-WWOX Antibody (M01223) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-fcm-testing-6.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) Flow Cytometry analysis of U20S cells using anti-WWOX antibody (M01223). <br>Overlay histogram showing U20S cells stained with M01223 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-WWOX Antibody (M01223, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01223-wwox-primary-antibodies-if-testing-7.jpgAnti-WWOX Antibody Picoband® (monoclonal, 3D10) IF analysis of WWOX using anti-WWOX antibody (M01223). <br>
WWOX was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-WWOX Antibody (M01223) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parn-picoband-trade-antibody-monoclonal-m01501-boster.html2026-03-24T05:21:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-wb-testing-1.jpgAnti-PARN Antibody Picoband® (monoclonal, 11D8) Western blot analysis of PARN using anti-PARN antibody (M01501).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: monkey COS-7 whole cell lysates,<br>
Lane 4: human CACO-2 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PARN antigen affinity purified monoclonal antibody (Catalog # M01501) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PARN at approximately 78KD. The expected band size for PARN is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-ihc-testing-2.jpgAnti-PARN Antibody Picoband® (monoclonal, 11D8) IHC analysis of PARN using anti PARN antibody (M01501). <br>
PARN was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PARN Antibody (M01501) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-if-testing-3.jpgAnti-PARN Antibody Picoband® (monoclonal, 11D8) IF analysis of PARN using anti-PARN antibody (M01501). <br>
PARN was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PARN Antibody (M01501) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-if-testing-4.jpgAnti-PARN Antibody Picoband® (monoclonal, 11D8) IF analysis of PARN using anti-PARN antibody (M01501). <br>
PARN was detected in immunocytochemical section of U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PARN Antibody (M01501) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-fcm-testing-5.pngAnti-PARN Antibody Picoband® (monoclonal, 11D8) Flow Cytometry analysis of A549 cells using anti-PARN antibody (M01501). <br>
Overlay histogram showing A549 cells stained with M01501 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PARN Antibody (M01501, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01501-parn-primary-antibodies-fcm-testing-6.pngAnti-PARN Antibody Picoband® (monoclonal, 11D8) Flow Cytometry analysis of A431 cells using anti-PARN antibody (M01501). <br>
Overlay histogram showing A431 cells stained with M01501 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PARN Antibody (M01501, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-optineurin-optn-picoband-trade-antibody-monoclonal-m00952-boster.html2026-03-24T05:21:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-optineurin-optn-primary-antibodies-wb-testing-1.jpgAnti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8) Western blot analysis of Optineurin/OPTN using anti-Optineurin/OPTN antibody (M00952).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human U-87MG whole cell lysates;<br>
Lane 2: human U20S whole cell lysates;<br>
Lane 3: human placenta tissue lysates;<br>
Lane 4: human Hela whole cell lysates;<br>
Lane 5: human A549 whole cell lysates;<br>
Lane 6: human K562 whole cell lysates;<br>
Lane 7: rat C6 whole cell lysates;<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Optineurin/OPTN antigen affinity purified monoclonal antibody (Catalog # M00952) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Optineurin/OPTN at approximately 66KD. The expected band size for Optineurin/OPTN is at 66KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif6-picoband-trade-antibody-monoclonal-m03581-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-wb-testing-1.jpgAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) Western blot analysis of EIF6 using anti-EIF6 antibody (M03581). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human CACO-2 whole cell lysates, <br>
Lane 3: human HEPG2 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
Lane 6: human PC-3 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates, <br>
Lane 8: human U87 whole cell lysates, <br>
Lane 9: rat kidney tissue lysates, <br>
Lane 10: rat testis tissue lysates, <br>
Lane 11: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EIF6 antigen affinity purified monoclonal antibody (Catalog # M03581) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EIF6 at approximately 26KD. The expected band size for EIF6 is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-ihc-testing-2.jpgAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) IHC analysis of EIF6 using anti-EIF6 antibody (M03581). <br>
EIF6 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EIF6 Antibody (M03581) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-ihc-testing-3.jpgAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) IHC analysis of EIF6 using anti-EIF6 antibody (M03581). <br>
EIF6 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EIF6 Antibody (M03581) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-ihc-testing-4.jpgAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) IHC analysis of EIF6 using anti-EIF6 antibody (M03581). <br>
EIF6 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EIF6 Antibody (M03581) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-ihc-testing-5.jpgAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) IHC analysis of EIF6 using anti-EIF6 antibody (M03581). <br>
EIF6 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EIF6 Antibody (M03581) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03581-eif6-primary-antibodies-fcm-testing-6.pngAnti-EIF6 Antibody Picoband® (monoclonal, 2I11) Flow Cytometry analysis of U20S cells using anti-EIF6 antibody (M03581). <br>Overlay histogram showing U20S cells stained with M03581 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF6 Antibody (M03581, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gnaq-picoband-trade-antibody-monoclonal-m00898-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-gnaq-primary-antibodies-wb-testing-1.jpgAnti-GNAQ Antibody Picoband® (monoclonal, 13H4) Western blot analysis of GNAQ using anti-GNAQ antibody (M00898).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human A549 whole cell lysates.<br>
Lane 4: human A431 whole cell lysates,<br>
Lane 5: human MCF-7 whole cell lysates,<br>
Lane 6: human K562 whole cell lysates,<br>
Lane 7: monkey COS-7 whole cell lysates,<br>
Lane 8: rat brain tissue lysates,<br>
Lane 9: rat lung tissue lysates,<br>
Lane 10: mouse brain tissue lysates,<br>
Lane 11: mouse lung tissue lysates,<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNAQ antigen affinity purified monoclonal antibody (Catalog # M00898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNAQ at approximately 42KD. The expected band size for GNAQ is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-gnaq-primary-antibodies-ihc-testing-2.jpgAnti-GNAQ Antibody Picoband® (monoclonal, 13H4) IHC analysis of GNAQ using anti GNAQ antibody (M00898). <br>
GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-gnaq-primary-antibodies-ihc-testing-3.jpgAnti-GNAQ Antibody Picoband® (monoclonal, 13H4) IHC analysis of GNAQ using anti GNAQ antibody (M00898). <br>
GNAQ was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-gnaq-primary-antibodies-ihc-testing-4.jpgAnti-GNAQ Antibody Picoband® (monoclonal, 13H4) IHC analysis of GNAQ using anti GNAQ antibody (M00898). <br>
GNAQ was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GNAQ Antibody (M00898) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-gnaq-primary-antibodies-fcm-testing-5.pngAnti-GNAQ Antibody Picoband® (monoclonal, 13H4) Flow Cytometry analysis of U20S cells using anti- GNAQ antibody (M00898). <br>Overlay histogram showing U20S cells stained with M00898 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GNAQ Antibody (M00898, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ca2-picoband-trade-antibody-monoclonal-m00143-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-wb-testing-1.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) Western blot analysis of CA2 using anti-CA2 antibody (M00143).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: rat heart tissue lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: rat spleen tissue lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: mouse heart tissue lysates,<br>
Lane 6: mouse kidney tissue lysates,<br>
Lane 7: mouse spleen tissue lysates,<br>
Lane 8: mouse liver tissue lysates,<br>
Lane 9: human placenta tissue lysates,<br>
Lane 10: human 293T whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CA2 antigen affinity purified monoclonal antibody (Catalog # M00143) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CA2 at approximately 28KD. The expected band size for CA2 is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-ihc-testing-2.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) IHC analysis of CA2 using anti-CA2 antibody (M00143). <br>
CA2 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CA2 Antibody (M00143) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-ihc-testing-3.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) IHC analysis of CA2 using anti-CA2 antibody (M00143). <br>
CA2 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CA2 Antibody (M00143) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-ihc-testing-4.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) IHC analysis of CA2 using anti-CA2 antibody (M00143). <br>
CA2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CA2 Antibody (M00143) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-ihc-testing-5.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) IHC analysis of CA2 using anti-CA2 antibody (M00143). <br>
CA2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CA2 Antibody (M00143) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-fcm-testing-6.pngAnti-CA2 Antibody Picoband® (monoclonal, 10E11) Flow Cytometry analysis of 293T cells using anti-CA2 antibody (M00143). <br>Overlay histogram showing 293T cells stained with M00143 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CA2 Antibody (M00143, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00143-ca2-primary-antibodies-if-testing-7.jpgAnti-CA2 Antibody Picoband® (monoclonal, 10E11) IF analysis of CA2 using anti-CA2 antibody (M00143). <br>
CA2 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-CA2 Antibody (M00143) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ara9-aip-picoband-trade-antibody-monoclonal-m02759-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-wb-testing-1.jpgAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) Western blot analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human HEPG2 whole cell lysates, <br>
Lane 3: human placenta tissue lysates, <br>
Lane 4: human CACO-2 whole cell lysates, <br>
Lane 5: human U20S whole cell lysates, <br>
Lane 6: monkey COS-7 whole cell lysates, <br>
Lane 7: human K562 whole cell lysates, <br>
Lane 8: rat heart tissue lysates, <br>
Lane 9: rat skeletal muscle tissue lysates, <br>
Lane 10: rat brain tissue lysates, <br>
Lane 11: rat spleen tissue lysates, <br>
Lane 12: mouse brain tissue lysates, <br>
Lane 13: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARA9/AIP antigen affinity purified monoclonal antibody (Catalog # M02759) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ARA9/AIP at approximately 38KD. The expected band size for ARA9/AIP is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-ihc-testing-2.jpgAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759). <br>
ARA9/AIP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-ihc-testing-3.jpgAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759). <br>
ARA9/AIP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-ihc-testing-4.jpgAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) IHC analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759). <br>
ARA9/AIP was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-if-testing-5.jpgAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) IF analysis of ARA9/AIP using anti-ARA9/AIP antibody (M02759). <br>
ARA9/AIP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ARA9/AIP Antibody (M02759) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02759-aip-primary-antibodies-fcm-testing-6.pngAnti-ARA9/AIP Antibody Picoband® (monoclonal, 10G8) Flow Cytometry analysis of A549 cells using anti-ARA9/AIP antibody (M02759). <br>Overlay histogram showing A549 cells stained with M02759 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ARA9/AIP Antibody (M02759, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rps6-picoband-trade-antibody-monoclonal-m01567-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-rps6-primary-antibodies-if-testing-1.jpgAnti-RPS6 Antibody Picoband® (monoclonal, 2H7) IF analysis of RPS6 using anti-RPS6 antibody (M01567) <br> RPS6 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-RPS6 Antibody (M01567) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab5-rab5a-picoband-trade-antibody-monoclonal-m01891-1-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-1-rab5a-primary-antibodies-if-testing-1.jpgAnti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9) IF analysis of RAB5A using anti-RAB5A antibody (M01891-1). <br>
RAB5A was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RAB5A Antibody (M01891-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-1-rab5a-primary-antibodies-wb-testing-2.jpgAnti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9) Western blot analysis of Rab5/RAB5A using anti-Rab5/RAB5A antibody (M01891-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human placenta tissue lysates,<br>
Lane 3: human A549 whole cell lysates.<br>
Lane 4: human A375 whole cell lysates,<br>
Lane 5: human SW620 whole cell lysates,<br>
Lane 6: human U20S whole cell lysates,<br>
Lane 7: human HEK293 whole cell lysates,<br>
Lane 8: human U87 whole cell lysates,<br>
Lane 9: rat brain tissue lysates,<br>
Lane 10: mouse brain tissue lysates,<br>
Lane 11: mouse Neuro-2a whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Rab5/RAB5A antigen affinity purified monoclonal antibody (Catalog # M01891-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Rab5/RAB5A at approximately 24KD. The expected band size for Rab5/RAB5A is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-1-rab5a-primary-antibodies-fcm-testing-3.pngAnti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9) Flow Cytometry analysis of A431 cells using anti-Rab5/RAB5A antibody (M01891-1). <br>Overlay histogram showing A431 cells stained with M01891-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Rab5/RAB5A Antibody (M01891-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-3-lgals3-picoband-trade-antibody-monoclonal-m00621-3-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-wb-testing-1.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (M00621-3).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates.<br>
Lane 4: human T47D whole cell lysates,<br>
Lane 5: human CACO-2 whole cell lysates,<br>
Lane 6: human placenta tissue lysates,<br>
Lane 7: human A549 whole cell lysates,<br>
Lane 8: human HL-60 whole cell lysates,<br>
Lane 9: rat lung tissue lysates,<br>
Lane 10: rat testis tissue lysates,<br>
Lane 11: mouse lung tissue lysates,<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Galectin 3/LGALS3 antigen affinity purified monoclonal antibody (Catalog # M00621-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 28KD. The expected band size for Galectin 3/LGALS3 is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-ihc-testing-2.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-ihc-testing-3.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-ihc-testing-4.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-ihc-testing-5.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IHC analysis of Galectin 3/LGALS3 using anti Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-if-testing-6.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-lgals3-primary-antibodies-fcm-testing-7_1.pngAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) Flow Cytometry analysis of U20S cells using anti-Galectin 3/LGALS3 antibody (M00621-3). <br>Overlay histogram showing U20S cells stained with M00621-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Galectin 3/LGALS3 Antibody (M00621-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-3-galectin-3-primary-antibodies-if-testing-8.jpgAnti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) IF analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (M00621-3). <br>
Galectin 3/LGALS3 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Galectin 3/LGALS3 Antibody (M00621-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-1-cav1-picoband-trade-antibody-monoclonal-m00179-1-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human A549 whole cell lysates;<br>
Lane 3: human placenta tissue lysates;<br>
Lane 4: human U-87 whole cell lysates;<br>
Lane 5: human PC-3 whole cell lysates;<br>
Lane 6: human U20S whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Caveolin-1/CAV1 antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22KD. The expected band size for Caveolin-1/CAV1 is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-40859510-figure-3.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7)Analysis of interacting proteins of LncRNA00602. (A) Bioinformatics results illustrating the potential binding interactions between LncRNA00602 and various proteins (including UPF1, UPF2, UPF3A, UPF3B, SMG1, SMG7, GSPT1, EIF, MAGOH, GSP, and CASC3); (B) RNA-IP experiment results; (C) Silver staining result; (D) Western blot results of Co-IP; (E) mass spectrometry results; (F) SMG1 mRNA expression; (G) UPF2 mRNA expression; (H) CAV-1 mRNA expression; (I) UPF1 mRNA expression. N = 3. Compared with control, *P < .05, **P < .01. CAV-1 = caveolin-1, Co-IP = co-immunoprecipitation, RNA-IP = RNA-immunoprecipitation.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://journals.lww.com/md-journal/fulltext/2025/08220/browning_effect_of_lncrna00602_in_ad36_induced.39.aspx'>40859510</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-40859510-figure-4.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7)Analysis of ATP5O, COX5B, CAV-1, and UCP1 expression after CAV-1 knockdown. RT-qPCR detected gene expression of CAV-1 (A), UCP1 (B), ATP5O (C), and COX5B (D). Protein expression was measured with Western blot. (E) Representative Western blot results. Relative protein expression of CAV-1 (F), UCP1 (G), ATP5O (H), and COX5B (I). N = 3. Compared with control, *P < .05, **P < .01. CAV-1 = caveolin-1, RT-qPCR = real-time quantitative PCR.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://journals.lww.com/md-journal/fulltext/2025/08220/browning_effect_of_lncrna00602_in_ad36_induced.39.aspx'>40859510</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-ihc-testing-3.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-ihc-testing-4.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-ihc-testing-5.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-ihc-testing-6.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-if-testing-7.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-if-testing-8.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-1-cav1-primary-antibodies-if-testing-9.jpgAnti-Caveolin-1/CAV1 Antibody Picoband® (monoclonal, 12C7) IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). <br>
Caveolin-1/CAV1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfia-picoband-trade-antibody-monoclonal-m03531-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-wb-testing-1.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) Western blot analysis of NFIA using anti-NFIA antibody (M03531).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NFIA antigen affinity purified monoclonal antibody (Catalog # M03531) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NFIA at approximately 62KD. The expected band size for NFIA is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-ihc-testing-2.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) IHC analysis of NFIA using anti-NFIA antibody (M03531). <br>
NFIA was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NFIA Antibody (M03531) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-ihc-testing-3.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) IHC analysis of NFIA using anti-NFIA antibody (M03531). <br>
NFIA was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NFIA Antibody (M03531) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-ihc-testing-4.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) IHC analysis of NFIA using anti-NFIA antibody (M03531). <br>
NFIA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NFIA Antibody (M03531) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-if-testing-5.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) IF analysis of NFIA using anti-NFIA antibody (M03531). <br>
NFIA was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-NFIA Antibody (M03531) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03531-nfia-primary-antibodies-fcm-testing-6.jpgAnti-NFIA Antibody Picoband® (monoclonal, 16H11) Flow Cytometry analysis of U20S cells using anti- NFIA antibody (M03531). <br>Overlay histogram showing U20S cells stained with M03531 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NFIA Antibody (M03531, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystatin-c-cst3-picoband-trade-antibody-monoclonal-m00961-1-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-if-testing-1.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) IF analysis of CST3 using anti-CST3 antibody (M00961-1). <br>
CST3 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CST3 Antibody (M00961-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-wb-testing-2.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) Western blot analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody (M00961-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human PC-3 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human placenta tissue lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: human COLO320 whole cell lysates,<br>
Lane 6: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cystatin C/CST3 antigen affinity purified monoclonal antibody (Catalog # M00961-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cystatin C/CST3 at approximately 15KD. The expected band size for Cystatin C/CST3 is at 15KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-ihc-testing-3.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody (M00961-1). <br>
Cystatin C/CST3 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cystatin C/CST3 Antibody (M00961-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-ihc-testing-4.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody (M00961-1). <br>
Cystatin C/CST3 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cystatin C/CST3 Antibody (M00961-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-ihc-testing-5.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) IHC analysis of Cystatin C/CST3 using anti-Cystatin C/CST3 antibody (M00961-1). <br>
Cystatin C/CST3 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cystatin C/CST3 Antibody (M00961-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-fcm-testing-6.pngAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) Flow Cytometry analysis of A549 cells using anti-Cystatin C/CST3 antibody (M00961-1). <br>Overlay histogram showing A549 cells stained with M00961-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cystatin C/CST3 Antibody (M00961-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-1-cst3-primary-antibodies-elisa-testing-7.jpgAnti-Cystatin C/CST3 Antibody Picoband® (monoclonal, 4H8) Sandwich ELISA - Recombinant human Cystatin C/CST3 protein standard curve.<br>
Use in combination with reagents from Human Cystatin C/CST3 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0678).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystatin-c-cst3-picoband-trade-antibody-monoclonal-m00961-3-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00961-3-cst3-primary-antibodies-elisa-testing-1.jpgAnti-Cystatin C/CST3 Antibody (monoclonal, 8H7) Sandwich ELISA - Recombinant human Cystatin C/CST3 protein standard curve.<br>
Use in combination with reagents from Human Cystatin C/CST3 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0678).
https://www.bosterbio.com/picokine-elisa-kits/human-jam-b-jam2-picokine-trade-elisa-kit-ek1842-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1842.pngHuman JAM-B/JAM2 ELISA Kit PicoKine®Human JAM-B/JAM2 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-reg1a-picokine-trade-elisa-kit-ek1812-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1812_1.pngHuman REG1A ELISA Kit PicoKine®Human REG1A PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-reg3a-picokine-trade-elisa-kit-ek1835-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1835.pngMouse REG3A ELISA Kit PicoKine®Mouse REG3A PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-ve-cadherin-cd144-picokine-trade-elisa-kit-ek1916-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1916.jpgRat VE-Cadherin Cdh5 ELISA Kit PicoKine®Rat VE-Cadherin/CD144 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-endoglycan-podxl2-picokine-trade-elisa-kit-ek1928-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1928.pngHuman Endoglycan/PODXL2 ELISA Kit PicoKine®Human Endoglycan/PODXL2 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-v-picokine-trade-elisa-kit-ek1929-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1929.jpgHuman Cathepsin V/CTSV ELISA Kit PicoKine®Human Cathepsin V PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-fcrl5-elisa-kit-picokine-trade-ek1935-boster.html2026-03-24T05:21:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1935.jpgHuman FCRL5 ELISA Kit PicoKine®Human FCRL5 PicoKine ELISA Kitstandard curve
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https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-33-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0929-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0929_1.pngHuman IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-33 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-10-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0417-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0417_1.pngMouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-10 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-cystatin-c-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0678-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0678.jpgHuman Cystatin C ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Cystatin C EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-crp-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1316-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1316.pngHuman CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human CRP EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-pcsk9-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1147-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1147_1.pngHuman PCSK9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human PCSK9 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-32-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1599-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1599_1.pngHuman IL-32 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-32 EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-urb-ccdc80-picokine-trade-elisa-kit-ek1962-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1962.jpgHuman URB/CCDC80 ELISA Kit PicoKine®Human URB/CCDC80 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-a6-picokine-trade-elisa-kit-ek1963-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1963.jpgHuman Serpin A6 ELISA Kit PicoKine®Human Serpin A6 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cystatin-d-cst5-picokine-trade-elisa-kit-ek1964-boster.html2026-04-03T05:00:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1964.pngHuman Cystatin D/CST5 ELISA Kit PicoKine®Human Cystatin D/CST5 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cystatin-m-cst6-picokine-trade-elisa-kit-ek1965-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1965.jpgHuman Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®Human Cystatin M/CST6 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-d1-picokine-trade-elisa-kit-ek1968-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1968.jpgHuman Serpin D1 ELISA Kit PicoKine®Human Serpin D1 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-serpin-d1-picokine-trade-elisa-kit-ek1969-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1969.jpgMouse Serpin D1 ELISA Kit PicoKine®Mouse Serpin D1 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-serpin-d1-picokine-trade-elisa-kit-ek1970-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1970.jpgRat Serpin D1 ELISA Kit PicoKine®Rat Serpin D1 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-proc-protein-c-picokine-trade-elisa-kit-ek1975-boster.html2026-03-24T05:21:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1975.jpgHuman PROC/Protein C ELISA Kit PicoKine®Human PROC/Protein C PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/human-flrt2-picokine-trade-elisa-kit-ek1971-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1971.pngHuman FLRT2 ELISA Kit PicoKine®Human FLRT2 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-flrt2-picokine-trade-elisa-kit-ek1972-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1972_1.pngMouse FLRT2 ELISA Kit PicoKine®Mouse FLRT2 PicoKine ELISA Kitstandard curve
https://www.bosterbio.com/picokine-elisa-kits/rat-flrt2-picokine-trade-elisa-kit-ek1973-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1973.pngRat FLRT2 ELISA Kit PicoKine®Rat FLRT2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-flrt3-picokine-trade-elisa-kit-ek1974-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1974.pngHuman FLRT3 ELISA Kit PicoKine®Human FLRT3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-fcrl2-picokine-trade-elisa-kit-ek1976-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1976_1.pngHuman FCRL2 ELISA Kit PicoKine®Human FCRL2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-fcrl5-picokine-trade-elisa-kit-ek1978-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1978.pngMouse FCRL5 ELISA Kit PicoKine®Mouse FCRL5 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-fcrl1-picokine-trade-elisa-kit-ek1977-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1977.pngMouse FCRL1 ELISA Kit PicoKine®Mouse FCRL1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd300f-picokine-trade-elisa-kit-ek1982-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1982.pngHuman CD300F ELISA Kit PicoKine®Human CD300F PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cilp-picokine-trade-elisa-kit-ek1986-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1986.jpgHuman CILP ELISA Kit PicoKine®Human CILP PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd300c-picokine-trade-elisa-kit-ek1981-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1981_1.pngHuman CD300C ELISA Kit PicoKine®Human CD300C PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-treml2-picokine-trade-elisa-kit-ek1985-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1985_1.pngHuman TREML2 ELISA Kit PicoKine®Human TREML2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-creld2-picokine-trade-elisa-kit-ek1983-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1983_1.pngHuman CRELD2 ELISA Kit PicoKine®Human CRELD2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd300a-picokine-trade-elisa-kit-ek1980-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1980_1.pngHuman CD300A ELISA Kit PicoKine®Human CD300A PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-creld2-picokine-trade-elisa-kit-ek1984-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1984.pngMouse CRELD2 ELISA Kit PicoKine®Mouse CRELD2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-il-32-picokine-trade-elisa-kit-ek1599-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1599_2.pngHuman IL-32/Interleukin-32 ELISA Kit PicoKine®Human IL-32 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-serpin-a12-picokine-trade-elisa-kit-ek1874-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1874.pngHuman Serpin A12 ELISA Kit PicoKine®Human Serpin A12 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-krt8-picoband-trade-antibody-monoclonal-m01421-3-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin_8-primary-antibodies-fc-testing-1.pngAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9)1. Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8 antibody (M01421-3). <br> Overlay histogram showing A549 cells stained with M01421-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 8 Antibody (M01421-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin-8-primary-antibodies-wb-testing-2.jpgAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (M01421-3).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates;<br>
Lane 2: human Caco-2 whole cell lysates;<br>
Lane 3: human A549 whole cell lysates;<br>
Lane 4: human A431 whole cell lysates;<br>
Lane 5: human Hela whole cell lysates;<br>
Lane 6: human K562 whole cell lysates;<br>
Lane 7: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 8 antigen affinity purified monoclonal antibody (Catalog # M01421-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD. The expected band size for Cytokeratin 8 is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin-8-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) IHC analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (M01421-3). <br>
Cytokeratin 8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cytokeratin 8 Antibody (M01421-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-Mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin-8-primary-antibodies-if-testing-4.jpgAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (M01421-3). <br>
Cytokeratin 8 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Cytokeratin 8 Antibody (M01421-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin-8-primary-antibodies-if-testing-5.jpgAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (M01421-3). <br>
Cytokeratin 8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Cytokeratin 8 Antibody (M01421-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-3-cytokeratin-8-primary-antibodies-if-testing-6.jpgAnti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) IF analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (M01421-3). <br>
Cytokeratin 8 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml mouse anti-Cytokeratin 8 Antibody (M01421-3) overnight at 4°C. Biotin Conjugated Goat anti-Mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Cy3 Conjugated Avidin (BA1037). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dhfr-picoband-trade-antibody-monoclonal-m00813-1-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-1-dhfr-primary-antibodies-wb-testing-1.jpgAnti-DHFR Antibody Picoband® (monoclonal, 3C8) Western blot analysis of DHFR using anti-DHFR antibody (M00813-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human SGC-7901 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human SW620 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHFR antigen affinity purified monoclonal antibody (Catalog # M00813-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DHFR at approximately 22KD. The expected band size for DHFR is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-1-dhfr-primary-antibodies-ihc-testing-2.jpgAnti-DHFR Antibody Picoband® (monoclonal, 3C8) IHC analysis of DHFR using anti-DHFR antibody (M00813-1). <br>
DHFR was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DHFR Antibody (M00813-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00813-1-dhfr-primary-antibodies-ihc-testing-3.jpgAnti-DHFR Antibody Picoband® (monoclonal, 3C8) IHC analysis of DHFR using anti-DHFR antibody (M00813-1). <br>
DHFR was detected in paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DHFR Antibody (M00813-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gpx1-picoband-trade-antibody-monoclonal-m01019-2-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-wb-testing-1.jpgAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) Western blot analysis of GPX1 using anti-GPX1 antibody (M01019-2).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human U-87 whole cell lysates,<br>
Lane 3: human THP-1 whole cell lysates,<br>
Lane 4: human U-937 whole cell lysates,<br>
Lane 5: human HepG2 whole cell lysates,<br>
Lane 6: rat brain tissue lysates,<br>
Lane 7: rat thymus tissue lysates,<br>
Lane 8: rat lung tissue lysates,<br>
Lane 9: rat liver tissue lysates,<br>
Lane 10: mouse brain tissue lysates,<br>
Lane 11: mouse thymus tissue lysates,<br>
Lane 12: mouse lung tissue lysates,<br>
Lane 13: mouse liver tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GPX1 antigen affinity purified monoclonal antibody (Catalog # M01019-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22KD. The expected band size for GPX1 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-ihc-testing-2_1.jpgAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) IHC analysis of GPX1 using anti GPX1 antibody (M01019-2). <br>
GPX1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GPX1 Antibody (M01019-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-ihc-testing-3.jpgAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) IHC analysis of GPX1 using anti GPX1 antibody (M01019-2). <br>
GPX1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GPX1 Antibody (M01019-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-fcm-testing-4.pngAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) Flow Cytometry analysis of U87 cells using anti- GPX1 antibody (M01019-2). <br>Overlay histogram showing U87 cells stained with M01019-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (M01019-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-fcm-testing-5.pngAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) Flow Cytometry analysis of U251 cells using anti- GPX1 antibody (M01019-2). <br>Overlay histogram showing U251 cells stained with M01019-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (M01019-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01019-2-gpx1-primary-antibodies-if-testing-6.jpgAnti-GPX1 Antibody Picoband® (monoclonal, 8B10) IF analysis of GPX1 using anti-GPX1 antibody (M01019-2). <br>
GPX1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. And then incubated with 5μg/ml mouse anti-GPX1 Antibody (M01019-2) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-golga2-picoband-trade-antibody-monoclonal-m05865-1-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-1-golga2-primary-antibodies-wb-testing-1.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3) Western blot analysis of GM130 using anti-GM130 antibody (M05865-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human CACO-2 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GM130 antigen affinity purified monoclonal antibody (Catalog # M05865-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GM130 at approximately 140KD. The expected band size for GM130 is at 140KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-1-golga2-primary-antibodies-ihc-testing-2.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3) IHC analysis of GM130 using anti-GM130 antibody (M05865-1). <br>
GM130 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GM130 Antibody (M05865-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-1-golga2-primary-antibodies-ihc-testing-3.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3) IHC analysis of GM130 using anti-GM130 antibody (M05865-1). <br>
GM130 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GM130 Antibody (M05865-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-1-golga2-primary-antibodies-if-testing-4.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3) IF analysis of GM130 using anti-GM130 antibody (M05865-1). <br>
GM130 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-GM130 Antibody (M05865-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-1-golga2-primary-antibodies-fcm-testing-5.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 4G3) Flow Cytometry analysis of A431 cells using anti- GM130 antibody (M05865-1). <br>Overlay histogram showing A431 cells stained with M05865-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GM130 Antibody (M05865-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-casp3-picoband-trade-antibody-monoclonal-m00334-6-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-6-caspase-3-primary-antibodies-wb-testing-1.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 8B6) Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-6).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates;<br>
Lane 2: human K562 whole cell lysates;<br>
Lane 3: human HepG2 whole cell lysates;<br>
Lane 4: human Raji whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Caspase-3 antigen affinity purified monoclonal antibody (Catalog # M00334-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 35KD. The expected band size for Caspase-3 is at 32KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-6-caspase-3-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 8B6) IHC analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-6). <br>
Caspase-3 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caspase-3 Antibody (M00334-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-6-caspase-3-primary-antibodies-ihc-testing-3.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 8B6) IHC analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-6). <br>
Caspase-3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caspase-3 Antibody (M00334-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-6-caspase-3-primary-antibodies-ihc-testing-4.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 8B6) IHC analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-6). <br>
Caspase-3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caspase-3 Antibody (M00334-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-6-caspase-3-primary-antibodies-fcm-testing-5_1.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 8B6) Flow Cytometry analysis of HepG2 cells using anti- Caspase-3 antibody (M00334-6). <br>Overlay histogram showing HepG2 cells stained with M00334-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Caspase-3 Antibody (M00334-6, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-casp3-picoband-trade-antibody-monoclonal-m00334-7-boster.html2026-03-24T05:21:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-7-caspase-3-primary-antibodies-wb-testing-1.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 15G8) Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-7).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates;<br>
Lane 2: human SW579 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Caspase-3 antigen affinity purified monoclonal antibody (Catalog # M00334-7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 20KD. The expected band size for Caspase-3 is at 32KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-7-caspase-3-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 15G8) IHC analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-7). <br>
Caspase-3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caspase-3 Antibody (M00334-7) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-7-caspase-3-primary-antibodies-ihc-testing-3.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 15G8) IHC analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-7). <br>
Caspase-3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Caspase-3 Antibody (M00334-7) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-7-caspase-3-primary-antibodies-fcm-testing-4.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 15G8) Flow Cytometry analysis of HepG2 cells using anti- Caspase-3 antibody (M00334-7). <br>Overlay histogram showing HepG2 cells stained with M00334-7 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Caspase-3 Antibody (M00334-7, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-7-caspase-3-primary-antibodies-if-testing-5.jpgAnti-Caspase-3 Antibody Picoband® (monoclonal, 15G8) IF analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-7). <br>
Caspase-3 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Caspase-3 Antibody (M00334-7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-snrpn-picoband-trade-antibody-monoclonal-m02173-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02173-snrpn-primary-antibodies-wb-testing-1.jpgAnti-SNRPN Antibody Picoband® (monoclonal, 6F12) Western blot analysis of SNRPN using anti-SNRPN antibody (M02173). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: rat brain tissue lysates, <br>
Lane 4: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SNRPN antigen affinity purified monoclonal antibody (Catalog # M02173) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 26KD. The expected band size for SNRPN is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02173-snrpn-primary-antibodies-fcm-testing-2.pngAnti-SNRPN Antibody Picoband® (monoclonal, 6F12) Flow Cytometry analysis of A549 cells using anti-SNRPN antibody (M02173). <br>Overlay histogram showing A549 cells stained with M02173 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- SNRPN Antibody (M02173, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-usp7-picoband-trade-antibody-monoclonal-m01239-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-wb-testing-1.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) Western blot analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates;<br>
Lane 2: human 22RV1 whole cell lysates;<br>
Lane 3: human K562 whole cell lysates;<br>
Lane 4: human Caco-2 whole cell lysates;<br>
Lane 5: human HepG2 whole cell lysates;<br>
Lane 6: human U-87MG whole cell lysates;<br>
Lane 7: rat testicular tissue lysates;<br>
Lane 8: mouse testicular tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HAUSP/USP7 antigen affinity purified monoclonal antibody (Catalog # M01239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HAUSP/USP7 at approximately 140KD. The expected band size for HAUSP/USP7 is at 128KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-ihc-testing-2.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1). <br>
HAUSP/USP7 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HAUSP/USP7 Antibody (M01239-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-ihc-testing-3.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1). <br>
HAUSP/USP7 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HAUSP/USP7 Antibody (M01239-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-ihc-testing-4.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1). <br>
HAUSP/USP7 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HAUSP/USP7 Antibody (M01239-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-ihc-testing-5.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1). <br>
HAUSP/USP7 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HAUSP/USP7 Antibody (M01239-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-if-testing-6.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) IF analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1). <br>
HAUSP/USP7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-HAUSP/USP7 Antibody (M01239-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01239-1-hausp-usp7-primary-antibodies-fcm-testing-7.jpgAnti-HAUSP/USP7 Antibody Picoband® (monoclonal, 5E2) Flow Cytometry analysis of HepG2 cells using anti- HAUSP/USP7 antibody (M01239-1). <br>Overlay histogram showing HepG2 cells stained with M01239-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HAUSP/USP7 Antibody (M01239-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stub1-picoband-trade-antibody-monoclonal-m01236-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-wb-testing-1.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) Western blot analysis of STUB1 using anti-STUB1 antibody (M01236).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human HEK293 whole cell lysates,<br>
Lane 2: monkey COS-7 whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates.<br>
Lane 4: human THP-1 whole cell lysates,<br>
Lane 5: human PC-3 whole cell lysates,<br>
Lane 6: human U87 whole cell lysates,<br>
Lane 7: rat heart tissue lysates,<br>
Lane 8: rat brain tissue lysates,<br>
Lane 9: mouse brain tissue lysates,<br>
Lane 10: mouse HEPA1-6 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STUB1 antigen affinity purified monoclonal antibody (Catalog # M01236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35KD. The expected band size for STUB1 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-ihc-testing-2.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) IHC analysis of STUB1 using anti STUB1 antibody (M01236). <br>
STUB1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-ihc-testing-3.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) IHC analysis of STUB1 using anti STUB1 antibody (M01236). <br>
STUB1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-ihc-testing-4.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) IHC analysis of STUB1 using anti STUB1 antibody (M01236). <br>
STUB1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-ihc-testing-5.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) IHC analysis of STUB1 using anti STUB1 antibody (M01236). <br>
STUB1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-ihc-testing-6.jpgAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) IHC analysis of STUB1 using anti STUB1 antibody (M01236). <br>
STUB1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STUB1 Antibody (M01236) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01236-stub1-primary-antibodies-fcm-testing-7.pngAnti-STUB1 Antibody Picoband® (monoclonal, 13I8) Flow Cytometry analysis of A549 cells using anti- STUB1 antibody (M01236). <br>Overlay histogram showing A549 cells stained with M01236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-STUB1 Antibody (M01236, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mrp2-abcc2-picoband-trade-antibody-a00974-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00974-1-abcc2-primary-antibodies-wb-testing-1_1.jpgAnti-MRP2/ABCC2 Antibody Picoband® Western blot analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP2/ABCC2 antigen affinity purified polyclonal antibody (Catalog # A00974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP2/ABCC2 at approximately 170-250 kDa. The expected band size for MRP2/ABCC2 is at 174 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00974-1-abcc2-primary-antibodies-ihc-testing-2.jpgAnti-MRP2/ABCC2 Antibody Picoband® IHC analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1). <br>
MRP2/ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP2/ABCC2 Antibody (A00974-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00974-1-abcc2-primary-antibodies-ihc-testing-3.jpgAnti-MRP2/ABCC2 Antibody Picoband® IHC analysis of MRP2/ABCC2 using anti-MRP2/ABCC2 antibody (A00974-1). <br>
MRP2/ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRP2/ABCC2 Antibody (A00974-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-d6-ackr2-picoband-trade-antibody-a05491-2-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05491-2-ACKR2-primary-antibodies-WB-testing-1.jpgAnti-D6/ACKR2 Antibody Picoband® Western blot analysis of ACKR2 using anti-ACKR2 antibody (A05491-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Raji whole cell lysates,<br>Lane 2: human Hela whole cell lysates,<br>Lane 3: human SW620 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR2 antigen affinity purified polyclonal antibody (Catalog # A05491-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACKR2 at approximately 55KD. The expected band size for ACKR2 is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05491-2-ACKR2-primary-antibodies-IHC-testing-2.jpgAnti-D6/ACKR2 Antibody Picoband® IHC analysis of ACKR2 using anti-ACKR2 antibody (A05491-2).<br>ACKR2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACKR2 Antibody (A05491-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-annexin-a3-anxa3-picoband-trade-antibody-a04796-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04796-anxa3-primary-antibodies-wb-testing-1_1.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® Western blot analysis of ANXA3 using anti-ANXA3 antibody (A04796). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: rat lung tissue lysates,<br>
Lane 5: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # A04796) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA3 at approximately 36 kDa. The expected band size for ANXA3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04796-ANXA3-primary-antibodies-IHC-testing-2.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).<br>ANXA3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANXA3 Antibody (A04796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04796-ANXA3-primary-antibodies-IHC-testing-3.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).<br>ANXA3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANXA3 Antibody (A04796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04796-ANXA3-primary-antibodies-IHC-testing-4.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).<br>ANXA3 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANXA3 Antibody (A04796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04796-ANXA3-primary-antibodies-IHC-testing-5.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).<br>ANXA3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANXA3 Antibody (A04796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04796-ANXA3-primary-antibodies-IHC-testing-6.jpgAnti-Annexin A3/ANXA3 Antibody Picoband® IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).<br>ANXA3 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANXA3 Antibody (A04796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-arntl2-picoband-trade-antibody-a05332-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05332-1-arntl2-primary-antibodies-wb-testing-1.jpgAnti-ARNTL2 Antibody Picoband® Western blot analysis of ARNTL2 using anti-ARNTL2 antibody (A05332-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human PC-3 whole cell lysates,<br>Lane 2: human A549 whole cell lysates,<br>Lane 3: human HepG2 whole cell lysates,<br>Lane 4: rat ovary tissue lysates,<br>Lane 5: mouse lung tissue lysates,<br>Lane 6: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARNTL2 antigen affinity purified polyclonal antibody (Catalog # A05332-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARNTL2 at approximately 71KD. The expected band size for ARNTL2 is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05332-1-arntl2-primary-antibodies-fc-testing-2.jpgAnti-ARNTL2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-ARNTL2 antibody (A05332-1). <br>Overlay histogram showing A431 cells stained with A05332-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARNTL2 Antibody (A05332-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05332-1-arntl2-primary-antibodies-fc-testing-3.jpgAnti-ARNTL2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ARNTL2 antibody (A05332-1). <br>Overlay histogram showing HL-60 cells stained with A05332-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARNTL2 Antibody (A05332-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-caveolin-3-cav3-picoband-trade-antibody-a00990-2-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00990-2-cav3-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-3/CAV3 Antibody Picoband® Western blot analysis of CAV3 using anti-CAV3 antibody (A00990-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human U-87MG whole cell lysates,<br>Lane 3: human Hela whole cell lysates,<br>Lane 4: rat stomach tissue lysates,<br>Lane 5: rat testis tissue lysates,<br>Lane 6: mouse stomach tissue lysates,<br>Lane 7: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 antigen affinity purified polyclonal antibody (Catalog # A00990-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV3 at approximately 22KD. The expected band size for CAV3 is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00990-2-cav3-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-3/CAV3 Antibody Picoband® IHC analysis of CAV3 using anti-CAV3 antibody (A00990-2). <br>
CAV3 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAV3 Antibody (A00990-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00990-2-cav3-primary-antibodies-if-testing-3_1.jpgAnti-Caveolin-3/CAV3 Antibody Picoband® IF analysis of CAV3 using anti-CAV3 antibody (A00990-2). <br>
CAV3 was detected in a paraffin-embedded section of human cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CAV3 Antibody (A00990-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00990-2-cav3-primary-antibodies-if-testing-4_1.jpgAnti-Caveolin-3/CAV3 Antibody Picoband® IF analysis of CAV3 using anti-CAV3 antibody (A00990-2). <br>
CAV3 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CAV3 Antibody (A00990-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00990-2-cav3-primary-antibodies-if-testing-5_1.jpgAnti-Caveolin-3/CAV3 Antibody Picoband® IF analysis of CAV3 using anti-CAV3 antibody (A00990-2). <br>
CAV3 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CAV3 Antibody (A00990-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ccdc36-picoband-trade-antibody-a14775-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14775-ccdc36-primary-antibodies-wb-testing-1.jpgAnti-CCDC36 Antibody Picoband® Western blot analysis of CCDC36 using anti-CCDC36 antibody (A14775). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human HL-60 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC36 antigen affinity purified polyclonal antibody (Catalog # A14775) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC36 at approximately 66KD. The expected band size for CCDC36 is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A14775-CCDC36-primary-antibodies-IHC-testing-2.jpgAnti-CCDC36 Antibody Picoband® IHC analysis of CCDC36 using anti-CCDC36 antibody (A14775).<br>CCDC36 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCDC36 Antibody (A14775) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A14775-CCDC36-primary-antibodies-IHC-testing-3.jpgAnti-CCDC36 Antibody Picoband® IHC analysis of CCDC36 using anti-CCDC36 antibody (A14775).<br>CCDC36 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCDC36 Antibody (A14775) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14775-ccdc36-primary-antibodies-if-testing-4.jpgAnti-CCDC36 Antibody Picoband® IF analysis of CCDC36 using anti-CCDC36 antibody (A14775). <br>
CCDC36 was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCDC36 Antibody (A14775) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd40l-cd40lg-picoband-trade-antibody-a01114-3-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01114-3-cd40lg-primary-antibodies-wb-testing-1.jpgAnti-CD40L/CD40LG Antibody Picoband® Western blot analysis of CD40LG using anti-CD40LG antibody (A01114-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG antigen affinity purified polyclonal antibody (Catalog # A01114-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40LG at approximately 36KD. The expected band size for CD40LG is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cd47-picoband-trade-antibody-a00360-2-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00360-2-fonc-13-1089090-g005.jpgAnti-CD47 Antibody Picoband®Cell differentiation trajectories in CRC obtained by pseudotime analysis. (A–D) tSNE map shows the results of the dimensionality reduction and clustering analysis of S112 (A) , S115 (B) , S114 (C) , and 927 (D) (up). Results of pseudotime cell trajectory in S112 (A) , S115 (B) , S114 (C) , and S927 (D) (down). (E) Twelve genes were screened by invasive modules. (F) The relationship of STC1 expression level and cancer stage/progression-free survival in colon cancer (up) and rectal cancer (down) from TCGA database. (G) The relationship of CES1 expression level and cancer stage in colon cancer (up) and rectal cancer (down) from TCGA database. (H) Immunohistochemical staining showed the expression of AKR1B1(left panel), STC1(middle panel), and CD47(right panel) in Mucosa and cancer(up) and Invasive margin(down) (n=45). The scale bars on the lower right are 100 µm. *P < 0.05, **P < 0.01 and ***P < 0.001. NS, not significant difference.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9928961/&orig_host=www.ncbi.nlm.nih.gov'>36816947</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00360-2-cd47-primary-antibodies-fcm-testing-3.jpgAnti-CD47 Antibody Picoband® Flow Cytometry analysis of human PBMC cells using anti-CD47 antibody (A00360-2). <br>Overlay histogram showing human PBMC cells stained with A00360-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD47 Antibody (A00360-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00360-2-cd47-primary-antibodies-wb-testing-1_1.jpgAnti-CD47 Antibody Picoband® Western blot analysis of CD47 using anti-CD47 antibody (A00360-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD47 antigen affinity purified polyclonal antibody (Catalog # A00360-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD47 at approximately 50 kDa. The expected band size for CD47 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00360-2-cd47-primary-antibodies-ihc-testing-2.jpgAnti-CD47 Antibody Picoband® IHC analysis of CD47 using anti-CD47 antibody (A00360-2). <br>
CD47 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD47 Antibody (A00360-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cpamd8-picoband-trade-antibody-a12898-a12898-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12898-cpamd8-primary-antibodies-wb-testing-1.jpgAnti-CPAMD8 Antibody Picoband® Western blot analysis of CPAMD8 using anti-CPAMD8 antibody (A12898). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human T-47D whole cell lysates,<br>Lane 3: human U2OS whole cell lysates,<br>Lane 4: human K562 whole cell lysates,<br>Lane 5: human THP-1 whole cell lysates,<br>Lane 6: monkey COS-7 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPAMD8 antigen affinity purified polyclonal antibody (Catalog # A12898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPAMD8 at approximately 207KD. The expected band size for CPAMD8 is at 207KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12898-CPAMD8-primary-antibodies-IHC-testing-2.jpgAnti-CPAMD8 Antibody Picoband® IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).<br>CPAMD8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12898-CPAMD8-primary-antibodies-IHC-testing-3.jpgAnti-CPAMD8 Antibody Picoband® IHC analysis of CPAMD8 using anti-CPAMD8 antibody (A12898).<br>CPAMD8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPAMD8 Antibody (A12898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-cxcr4-picoband-trade-antibody-a00031-2-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00031-2-CXCR4-primary-antibodies-WB-testing-1.jpgAnti-CXCR4 Antibody Picoband® Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-2).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human HEK293 whole cell lysates,<br>Lane 2: human PC-3 whole cell lysates,<br>Lane 3: human Hela whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 36-40KD. The expected band size for CXCR4 is at 40KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dars2-picoband-trade-antibody-a06034-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-dars2-primary-antibodies-wb-testing-1.jpgAnti-DARS2 Antibody Picoband® Western blot analysis of DARS2 using anti-DARS2 antibody (A06034-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A431 whole cell lysates,<br>Lane 2: human K562 whole cell lysates,<br>Lane 3: human A549 whole cell lysates,<br>Lane 4: human PC-3 whole cell lysates,<br>Lane 5: human U2OS whole cell lysates,<br>Lane 6: human Caco-2 whole cell lysates,<br>Lane 7: human HEK293 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARS2 antigen affinity purified polyclonal antibody (Catalog # A06034-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DARS2 at approximately 74KD. The expected band size for DARS2 is at 74KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g001.jpgAnti-DARS2 Antibody Picoband®DARS2 Expression in BLCA. (A) DARS2 Pan-Cancer Analysis. (B) TCGA Analysis of DARS2 Expression in BLCA. (C) The expression of DARS2 in paired bladder cancer in TCGA. (D) qPCR analysis of DARS2 expression in 5 pairs of bladder cancer tissues. (E) Expression of DARS2 in bladder cancer cells and normal urothelial cells by western blot. (F) Western blot analysis of DARS2 expression in 5 pairs of bladder cancer tissues. (G) Immunohistochemistry of DARS2 and Ki67 in bladder cancer tissues. (H) Immunohistochemical analysis of DARS2 in 10 pairs of bladder cancer tissues. *P<0.05, **P<0.01, ***P<0.001. ns, no statistical difference.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g002.jpgAnti-DARS2 Antibody Picoband®DARS2 as an Independent Prognostic Factor in BLCA. (A) Kaplan-Meier analysis of OS in the TCGA BLCA. (B) Kaplan-Meier analysis of OS in the 37 cases of BLCA immunohistochemistry. (C) DARS2 expression distribution and survival status.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g003.jpgAnti-DARS2 Antibody Picoband®Identification and Enrichment Analysis of Differentially Expressed Genes. (A) Volcano plot for differentially expressed genes between high and low expression of DARS2 in BLCA patients. (B) GSEA revealed the top five are positively correlated. (C) GSEA revealed the top five are poorly correlated.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g004.jpgAnti-DARS2 Antibody Picoband®DARS2 Regulates Bladder Cancer Cell Proliferation, Migration, and Invasion. (A) DARS2 knockdown in T24 and EJ cells. (B, C) The impact of DARS2 on proliferation in T24 and EJ cells via CCK8, EDU. (D) The impact of DARS2 on migration and invasion in T24 and EJ cells. *P<0.05, **P<0.01, ***P<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g005.jpgAnti-DARS2 Antibody Picoband®Bladder cancer cells and Jurkat cells co-cultured. (A, B) PD-L1 changes after interference and overexpression of DARS2. (C) Relative concentration of IL-2 in culture medium after activation of jurkat cells by PMA and PHA. (D) Relative concentration of IL-2 in the culture medium after co-culture of activated Jurkat cells and untreated bladder cancer cells. (E, G) Relative concentration of IL-2 in the co-culture system after knocking down and overexpressing DRAS2. (F, H) Viability of residual surviving tumor cells in co-culture system after knockdown and overexpression of DRAS2 (I, J) Crystal violet staining of remaining surviving tumor cells in the co-culture system after knocking down and overexpressing DRAS2. (K) Expression of DARS2 and PD-L1 in bladder cancer cells and normal urothelial cells by western blot and analysis of the correlation between shigeDARS2 and PD-L1. *P<0.05, **P<0.01, ***P<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-fimmu-14-1301945-g006.jpgAnti-DARS2 Antibody Picoband®(A, B) subcutaneous tumor formation in nude mice. (C) Immunohistochemical staining of DARS2, PD-L1 and Ki67 in subcutaneous tumor samples from nude mice. *P<0.05, **P<0.01, ***P<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10827901/&orig_host=www.ncbi.nlm.nih.gov'>38299141</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06034-1-DARS2-primary-antibodies-IHC-testing-2.jpgAnti-DARS2 Antibody Picoband® IHC analysis of DARS2 using anti-DARS2 antibody (A06034-1).<br>DARS2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DARS2 Antibody (A06034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06034-1-DARS2-primary-antibodies-IHC-testing-3.jpgAnti-DARS2 Antibody Picoband® IHC analysis of DARS2 using anti-DARS2 antibody (A06034-1).<br>DARS2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DARS2 Antibody (A06034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06034-1-DARS2-primary-antibodies-IHC-testing-4.jpgAnti-DARS2 Antibody Picoband® IHC analysis of DARS2 using anti-DARS2 antibody (A06034-1).
<br>DARS2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DARS2 Antibody (A06034-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-dars2-primary-antibodies-if-testing-5.jpgAnti-DARS2 Antibody Picoband® IF analysis of DARS2 using anti-DARS2 antibody (A06034-1). <br>
DARS2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DARS2 Antibody (A06034-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-dars2-primary-antibodies-if-testing-6.jpgAnti-DARS2 Antibody Picoband® IF analysis of DARS2 using anti-DARS2 antibody (A06034-1). <br>
DARS2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DARS2 Antibody (A06034-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06034-1-dars2-primary-antibodies-fcm-testing-7.jpgAnti-DARS2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-DARS2 antibody (A06034-1).<br>Overlay histogram showing K562 cells stained with A06034-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DARS2 Antibody (A06034-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-egfl6-picoband-trade-antibody-a08756-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08756-egfl6-primary-antibodies-wb-testing-1.jpgAnti-EGFL6 Antibody Picoband® Western blot analysis of EGFL6 using anti-EGFL6 antibody (A08756). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human U-87MG whole cell lysates,<br>Lane 2: human A431 whole cell lysates,<br>Lane 3: human HL-60 whole cell lysates,<br>Lane 4: human K562 whole cell lysates,<br>Lane 5: human THP-1 whole cell lysates,<br>Lane 6: human Hela whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFL6 antigen affinity purified polyclonal antibody (Catalog # A08756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFL6 at approximately 62KD. The expected band size for EGFL6 is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08756-egfl6-primary-antibodies-wb-testing-2.jpgAnti-EGFL6 Antibody Picoband® Western blot analysis of EGFL6 using anti-EGFL6 antibody (A08756). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates,<br>Lane 2: mouse thymus tissue lysates,<br>Lane 3: mouse lung tissue lysates,<br>Lane 4: mouse liver tissue lysates,<br>Lane 5: mouse SP20 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFL6 antigen affinity purified polyclonal antibody (Catalog # A08756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFL6 at approximately 62KD. The expected band size for EGFL6 is at 62KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hdac5-picoband-trade-antibody-a01230-5-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01230-5-hdac5-primary-antibodies-wb-testing-1.jpgAnti-HDAC5 Antibody Picoband® Western blot analysis of HDAC5 using anti-HDAC5 antibody (A01230-5). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human THP-1 whole cell lysates,<br>Lane 4: human K562 whole cell lysates,<br>Lane 5: human PC-3 whole cell lysates,<br>Lane 6: human Caco-2 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC5 antigen affinity purified polyclonal antibody (Catalog # A01230-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC5 at approximately 150KD. The expected band size for HDAC5 is at 122KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01230-5-HDAC5-primary-antibodies-WB-testing-2.jpgAnti-HDAC5 Antibody Picoband® Western blot analysis of HDAC5 using anti-HDAC5 antibody (A01230-5). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat heart tissue lysates,<br>Lane 2: rat liver tissue lysates,<br>Lane 3: rat stomach tissue lysates,<br>Lane 4: rat ovary tissue lysates,<br>Lane 5: mouse heart tissue lysates,<br>Lane 6: mouse liver tissue lysates,<br>Lane 7: mouse stomach tissue lysates,<br>Lane 8: mouse ovary tissue lysates,<br>Lane 9: mouse NIH3T3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC5 antigen affinity purified polyclonal antibody (Catalog # A01230-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC5 at approximately 150KD. The expected band size for HDAC5 is at 122KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01230-5-HDAC5-primary-antibodies-IHC-testing-3.jpgAnti-HDAC5 Antibody Picoband® IHC analysis of HDAC5 using anti-HDAC5 antibody (A01230-5).<br>HDAC5 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC5 Antibody (A01230-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01230-5-HDAC5-primary-antibodies-IHC-testing-4.jpgAnti-HDAC5 Antibody Picoband® IHC analysis of HDAC5 using anti-HDAC5 antibody (A01230-5).<br>HDAC5 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC5 Antibody (A01230-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01230-5-HDAC5-primary-antibodies-IHC-testing-5.jpgAnti-HDAC5 Antibody Picoband® IHC analysis of HDAC5 using anti-HDAC5 antibody (A01230-5).<br>HDAC5 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC5 Antibody (A01230-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-hook2-picoband-trade-antibody-a08854-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08854-1-hook2-primary-antibodies-wb-testing-1.jpgAnti-HOOK2 Antibody Picoband® Western blot analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human RT4 whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK2 antigen affinity purified polyclonal antibody (Catalog # A08854-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK2 at approximately 83 kDa. The expected band size for HOOK2 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08854-1-hook2-primary-antibodies-ihc-testing-2.jpgAnti-HOOK2 Antibody Picoband® IHC analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). <br>
HOOK2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK2 Antibody (A08854-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08854-1-hook2-primary-antibodies-ihc-testing-3.jpgAnti-HOOK2 Antibody Picoband® IHC analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). <br>
HOOK2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK2 Antibody (A08854-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08854-1-hook2-primary-antibodies-if-testing-4.jpgAnti-HOOK2 Antibody Picoband® IF analysis of HOOK2 using anti-HOOK2 antibody (A08854-1). <br>
HOOK2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HOOK2 Antibody (A08854-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08854-1-hook2-primary-antibodies-fc-testing-5.pngAnti-HOOK2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-HOOK2 antibody (A08854-1). <br> Overlay histogram showing U20S cells stained with A08854-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOOK2 Antibody (A08854-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hook3-picoband-trade-antibody-a07701-1-boster.html2026-03-24T05:21:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07701-1-hook3-primary-antibodies-wb-testing-1.jpgAnti-HOOK3 Antibody Picoband® Western blot analysis of HOOK3 using anti-HOOK3 antibody (A07701-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MOLT-4 whole cell lysates,<br>
Lane 2: human RT4 whole cell lysates,<br>
Lane 3: human SiHa whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOOK3 antigen affinity purified polyclonal antibody (Catalog # A07701-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOOK3 at approximately 83 kDa. The expected band size for HOOK3 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-2.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).<br>HOOK3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-3.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).<br>HOOK3 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-4.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).<br>HOOK3 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-5.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).<br>HOOK3 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-6.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).<br>HOOK3 was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07701-1-HOOK3-primary-antibodies-IHC-testing-7.jpgAnti-HOOK3 Antibody Picoband® IHC analysis of HOOK3 using anti-HOOK3 antibody (A07701-1).
<br>HOOK3 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07701-1-hook3-primary-antibodies-if-testing-8.pngAnti-HOOK3 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-HOOK3 antibody (A07701-1). <br> Overlay histogram showing U87 cells stained with A07701-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOOK3 Antibody (A07701-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07701-1-hook3-primary-antibodies-if-testing-9.jpgAnti-HOOK3 Antibody Picoband® IF analysis of HOOK3 using anti-HOOK3 antibody (A07701-1). <br>
HOOK3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HOOK3 Antibody (A07701-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-haptoglobin-hp-picoband-trade-antibody-a00062-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00062-1-hp-primary-antibodies-wb-testing-1.jpgAnti-Haptoglobin/HP Antibody Picoband® Western blot analysis of HP using anti-HP antibody (A00062-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP antigen affinity purified polyclonal antibody (Catalog # A00062-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP at approximately 45KD. The expected band size for HP is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00062-1-HP-primary-antibodies-IHC-testing-2.jpgAnti-Haptoglobin/HP Antibody Picoband® IHC analysis of HP using anti-HP antibody (A00062-1).<br>HP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HP Antibody (A00062-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00062-1-HP-primary-antibodies-IHC-testing-3.jpgAnti-Haptoglobin/HP Antibody Picoband® IHC analysis of HP using anti-HP antibody (A00062-1).<br>HP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HP Antibody (A00062-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00062-1-HP-primary-antibodies-IHC-testing-4.jpgAnti-Haptoglobin/HP Antibody Picoband® IHC analysis of HP using anti-HP antibody (A00062-1).
<br>HP was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HP Antibody (A00062-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-mr1-antibody-a00618-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00618-1-mr1-primary-antibodies-wb-testing-1.jpgAnti-MR1 Antibody Picoband® Western blot analysis of MR1 using anti-MR1 antibody (A00618-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human T-47D whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human A431 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MR1 antigen affinity purified polyclonal antibody (Catalog # A00618-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MR1 at approximately 40KD. The expected band size for MR1 is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00618-1-mr1-primary-antibodies-fc-testing-2.pngAnti-MR1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-MR1 antibody (A00618-1). <br>Overlay histogram showing SiHa cells stained with A00618-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MR1 Antibody (A00618-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pomt2-picoband-trade-antibody-a04876-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04876-1-pomt2-primary-antibodies-wb-testing-1.jpgAnti-POMT2 Antibody Picoband® Western blot analysis of POMT2 using anti-POMT2 antibody (A04876-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human PC-3 whole cell lysates,<br>Lane 2: human Caco-2 whole cell lysates,<br>Lane 3: human HepG2 whole cell lysates,<br>Lane 4: rat lung tissue lysates,<br>Lane 5: rat testis tissue lysates,<br>Lane 6: rat spleen tissue lysates,<br>Lane 7: mouse lung tissue lysates,<br>Lane 8: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMT2 antigen affinity purified polyclonal antibody (Catalog # A04876-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMT2 at approximately 84KD. The expected band size for POMT2 is at 84KD.
https://www.bosterbio.com/products/primary-antibodies/anti-pyrophosphatase-1-ppa1-picoband-trade-antibody-a07485-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-wb-testing-1_1.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® Western blot analysis of PPA1 using anti-PPA1 antibody (A07485-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U937 whole cell lysates, <br>
Lane 2: human CACO-2 whole cell lysates, <br>
Lane 3: human Raji whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human HEK293 whole cell lysates, <br>
Lane 6: human HL-60 whole cell lysates, <br>
Lane 7: human K562 whole cell lysates, <br>
Lane 8: rat liver tissue lysates, <br>
Lane 9: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPA1 antigen affinity purified polyclonal antibody (Catalog # A07485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPA1 at approximately 33 kDa. The expected band size for PPA1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-ihc-testing-2.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® IHC analysis of PPA1 using anti-PPA1 antibody (A07485-1).<br>PPA1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPA1 Antibody (A07485-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-ihc-testing-3.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® IHC analysis of PPA1 using anti-PPA1 antibody (A07485-1).<br>PPA1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPA1 Antibody (A07485-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-ihc-testing-4.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® IHC analysis of PPA1 using anti-PPA1 antibody (A07485-1).<br>PPA1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPA1 Antibody (A07485-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-ihc-testing-5.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® IHC analysis of PPA1 using anti-PPA1 antibody (A07485-1).<br>PPA1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPA1 Antibody (A07485-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07485-1-ppa1-primary-antibodies-ihc-testing-6.jpgAnti-Pyrophosphatase 1/PPA1 Antibody Picoband® IHC analysis of PPA1 using anti-PPA1 antibody (A07485-1).
<br>PPA1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPA1 Antibody (A07485-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-scn4b-picoband-trade-antibody-a06453-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06453-1-scn4b-primary-antibodies-wb-testing-1.jpgAnti-SCN4B Antibody Picoband® Western blot analysis of SCN4B using anti-SCN4B antibody (A06453-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human U-87MG whole cell lysates,<br>Lane 3: monkey COS-7 whole cell lysates,<br>Lane 4: human U2OS whole cell lysates,<br>Lane 5: human HEK293 whole cell lysates,<br>Lane 6: human SHG-44 whole cell lysates,<br>Lane 7: human K562 whole cell lysates (negative control),<br>Lane 8: human HL-60 whole cell lysates (negative control). <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN4B antigen affinity purified polyclonal antibody (Catalog # A06453-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN4B at approximately 28KD. The expected band size for SCN4B is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06453-1-scn4b-primary-antibodies-wb-testing-2.jpgAnti-SCN4B Antibody Picoband® Western blot analysis of SCN4B using anti-SCN4B antibody (A06453-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat heart tissue lysates,<br>Lane 3: rat spleen tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: mouse brain tissue lysates,<br>Lane 6: mouse heart tissue lysates,<br>Lane 7: mouse spleen tissue lysates,<br>Lane 8: mouse kidney tissue lysates,<br>Lane 9: mouse Neuro-2a whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN4B antigen affinity purified polyclonal antibody (Catalog # A06453-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN4B at approximately 28KD. The expected band size for SCN4B is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06453-1-SCN4B-primary-antibodies-IHC-testing-3.jpgAnti-SCN4B Antibody Picoband® IHC analysis of SCN4B using anti-SCN4B antibody (A06453-1).<br>SCN4B was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SCN4B Antibody (A06453-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06453-1-SCN4B-primary-antibodies-IHC-testing-4.jpgAnti-SCN4B Antibody Picoband® IHC analysis of SCN4B using anti-SCN4B antibody (A06453-1).
<br>SCN4B was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SCN4B Antibody (A06453-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06453-1-scn4b-primary-antibodies-fc-testing-5.pngAnti-SCN4B Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SCN4B antibody (A06453-1). <br> Overlay histogram showing U20S cells stained with A06453-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN4B Antibody (A06453-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06453-1-scn4b-primary-antibodies-fc-testing-6.pngAnti-SCN4B Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SCN4B antibody (A06453-1). <br> Overlay histogram showing SiHa cells stained with A06453-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN4B Antibody (A06453-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sf3b4-picoband-trade-antibody-a06414-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06414-1-sf3b4-primary-antibodies-wb-testing-1_1.jpgAnti-SF3B4 Antibody Picoband® Western blot analysis of SF3B4 using anti-SF3B4 antibody (A06414-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human U2OS whole cell lysates,<br>
Lane 3: human HGC-27 whole cell lysates,<br>
Lane 4: human GES-1 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat stomach tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse stomach tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B4 antigen affinity purified polyclonal antibody (Catalog # A06414-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B4 at approximately 44 kDa. The expected band size for SF3B4 is at 44 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-sirt4-picoband-trade-antibody-a03764-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-WB-testing-1.jpgAnti-SIRT4 Antibody Picoband® Western blot analysis of SIRT4 using anti-SIRT4 antibody (A03764-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat heart tissue lysates,<br>Lane 3: rat liver tissue lysates,<br>Lane 4: mouse brain tissue lysates,<br>Lane 5: mouse heart tissue lysates,<br>Lane 6: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT4 antigen affinity purified polyclonal antibody (Catalog # A03764-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT4 at approximately 38KD. The expected band size for SIRT4 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-2.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).<br>SIRT4 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-3.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).<br>SIRT4 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-4.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).<br>SIRT4 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-5.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).<br>SIRT4 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-6.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).<br>SIRT4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03764-1-SIRT4-primary-antibodies-IHC-testing-7.jpgAnti-SIRT4 Antibody Picoband® IHC analysis of SIRT4 using anti-SIRT4 antibody (A03764-1).
<br>SIRT4 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SIRT4 Antibody (A03764-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/anti-slc34a2-picoband-trade-antibody-a03957-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03957-1-slc34a2-primary-antibodies-wb-testing-1_1.jpgAnti-SLC34A2 Antibody Picoband® Western blot analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates.<br>
a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC34A2 antigen affinity purified polyclonal antibody (A03957-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC34A2 at approximately 130 kDa. The expected band size for SLC34A2 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03957-1-slc34a2-primary-antibodies-ihc-testing-2.jpgAnti-SLC34A2 Antibody Picoband® IHC analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). <br>SLC34A2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC34A2 Antibody (A03957-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03957-1-slc34a2-primary-antibodies-ihc-testing-3.jpgAnti-SLC34A2 Antibody Picoband® IHC analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). <br>SLC34A2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC34A2 Antibody (A03957-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03957-1-slc34a2-primary-antibodies-ihc-testing-4.jpgAnti-SLC34A2 Antibody Picoband® IHC analysis of SLC34A2 using anti-SLC34A2 antibody (A03957-1). <br>SLC34A2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC34A2 Antibody (A03957-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-srcin1-picoband-trade-antibody-a08110-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08110-1-srcin1-primary-antibodies-wb-testing-1.jpgAnti-SRCIN1 Antibody Picoband® Western blot analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human T-47D whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRCIN1 antigen affinity purified polyclonal antibody (Catalog # A08110-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRCIN1 at approximately 140KD. The expected band size for SRCIN1 is at 140KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-2.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-3.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-4.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-5.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-6.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-7.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-8.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08110-1-SRCIN1-primary-antibodies-IHC-testing-9.jpgAnti-SRCIN1 Antibody Picoband® IHC analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1).<br>SRCIN1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SRCIN1 Antibody (A08110-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08110-1-srcin1-primary-antibodies-fc-testing-10.pngAnti-SRCIN1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SRCIN1 antibody (A08110-1). <br> Overlay histogram showing U20S cells stained with A08110-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRCIN1 Antibody (A08110-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08110-1-srcin1-primary-antibodies-fc-testing-11.pngAnti-SRCIN1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SRCIN1 antibody (A08110-1). <br> Overlay histogram showing A549 cells stained with A08110-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRCIN1 Antibody (A08110-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-taf12-picoband-trade-antibody-a06944-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06944-1-taf12-primary-antibodies-wb-testing-1.jpgAnti-TAF12 Antibody Picoband® Western blot analysis of TAF12 using anti-TAF12 antibody (A06944-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human U-937 whole cell lysates,<br>Lane 3: human K562 whole cell lysates,<br>Lane 4: human THP-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF12 antigen affinity purified polyclonal antibody (Catalog # A06944-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF12 at approximately 22KD. The expected band size for TAF12 is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06944-1-TAF12-primary-antibodies-IHC-testing-2.jpgAnti-TAF12 Antibody Picoband® IHC analysis of TAF12 using anti-TAF12 antibody (A06944-1).<br>TAF12 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TAF12 Antibody (A06944-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06944-1-TAF12-primary-antibodies-IHC-testing-3.jpgAnti-TAF12 Antibody Picoband® IHC analysis of TAF12 using anti-TAF12 antibody (A06944-1).<br>TAF12 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TAF12 Antibody (A06944-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06944-1-TAF12-primary-antibodies-IHC-testing-4.jpgAnti-TAF12 Antibody Picoband® IHC analysis of TAF12 using anti-TAF12 antibody (A06944-1).<br>TAF12 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TAF12 Antibody (A06944-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06944-1-TAF12-primary-antibodies-IHC-testing-5.jpgAnti-TAF12 Antibody Picoband® IHC analysis of TAF12 using anti-TAF12 antibody (A06944-1).<br>TAF12 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TAF12 Antibody (A06944-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tie2-tek-picoband-trade-antibody-a01274-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01274-1-tek-primary-antibodies-wb-testing-1.jpgAnti-TIE2/TEK Antibody Picoband® Western blot analysis of TEK using anti-TEK antibody (A01274-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human Caco-2 whole cell lysates,<br>Lane 3: human Hela whole cell lysates,<br>Lane 4: rat liver tissue lysates,<br>Lane 5: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-2.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-3.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-4.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-5.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-6.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-7.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-1-TEK-primary-antibodies-IHC-testing-8.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-1).<br>TEK was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>
https://www.bosterbio.com/products/primary-antibodies/anti-tmem199-antibody-a14686-1-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14686-1-tmem199-primary-antibodies-wb-testing-1.jpgAnti-Transmembrane protein 199 TMEM199 Antibody Picoband® Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>Lane 2: human placenta tissue lysates,<br>Lane 3: human Caco-2 whole cell lysates,<br>Lane 4: human T-47D whole cell lysates,<br>Lane 5: human U-87MG whole cell lysates,<br>Lane 6: human K562 whole cell lysates,<br>Lane 7: human U2OS whole cell lysates,<br>Lane 8: human PC-3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14686-1-gr2_lrg.jpgAnti-Transmembrane protein 199 TMEM199 Antibody Picoband®Nuclear localization of TMEM199. <br>(A) Gene Ontology -Cellular Component (GOCC) analysis of TMEM199 interacted proteins (TIPs). TIPs are generated by co-IP/MS assay.<br>
(B) Immunofluorescence assay to show nuclear localization in human osteosarcoma cell 143B, colorectal carcinoma cell line HCT116 as well as ovarian cancer cell line Caov-3, respectively. Photos are taken by confocal microscopy.<br>
(C) 3D images of nuclear TMEM199, taken and reconstructed by Confocal Microscopy.<br>
(D) Immunofluorescence assay to show the flag signal location when cells exogenously expressed TMEM-Flag protein.<br>
(E) Live cell imaging to show the GFP signal in HEK293 cells with TMEM199-GFP plasmid transfection.<br>
(F) Western blotting assay shows the cellular fracture TMEM199 containing content. CE, Cytoplasmic extraction; NFP, Nuclear free protein; DBP, DND binding protein.<br>
(G) Immunofluorescence assay to show the cellular localized signals of truncated TMEM199 fractions.<br> <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(24)02712-3?uuid=uuid%3A7962d8c8-d9ab-4387-bd8b-26a2642db570'>39758995</a></b>
https://www.bosterbio.com/picokine-elisa-kits/human-stc2-picokine-trade-elisa-kit-ek1988-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1988.jpgHuman STC2 / Stanniocalcin 2 ELISA Kit PicoKine®Human STC2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-stc2-picokine-trade-elisa-kit-ek1989-boster.html2026-03-27T05:07:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1989.pngMouse STC2/Stanniocalcin 2 ELISA Kit PicoKine®Mouse STC2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-stc2-picokine-trade-elisa-kit-ek1995-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1995.pngRat STC2/Stanniocalcin 2 ELISA Kit PicoKine®Rat STC2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-ifnar1-picokine-trade-elisa-kit-ek1991-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1991.pngMouse IFNAR1 ELISA Kit PicoKine®Mouse IFNAR1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-fut8-picokine-trade-elisa-kit-ek1990-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1990.pngHuman FUT8 ELISA Kit PicoKine®Human FUT8 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-gas6-picokine-trade-elisa-kit-ek1992-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1992.pngHuman GAS6 ELISA Kit PicoKine®Human GAS6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-myostatin-gdf8-picokine-trade-elisa-kit-ek1265-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1265_1.pngHuman Myostatin/GDF8 ELISA Kit PicoKine®Human Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-myostatin-gdf8-picokine-trade-elisa-kit-ek1266-boster.html2026-03-24T05:21:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1266.pngMouse Myostatin/GDF8 ELISA Kit PicoKine®Mouse Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-myostatin-gdf8-picokine-trade-elisa-kit-ek1267-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1267.pngRat Myostatin/GDF8 ELISA Kit PicoKine®Rat Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-sema3f-picokine-trade-elisa-kit-ek1734-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1734.jpgMouse SEMA3F/Semaphorin-3F ELISA Kit PicoKine®Mouse SEMA3F PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-sema3f-picokine-trade-elisa-kit-ek1735-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1735.pngRat SEMA3F/Semaphorin-3F ELISA Kit PicoKine®Rat SEMA3F PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-angptl6-picokine-trade-elisa-kit-ek1993-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1993.pngHuman ANGPTL6 ELISA Kit PicoKine®Human ANGPTL6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-scgb3a1-picokine-trade-elisa-kit-ek1994-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1994.pngRat SCGB3A1 ELISA Kit PicoKine®Rat SCGB3A1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-pzp-picokine-trade-elisa-kit-ek1873-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1873.pngHuman Pregnancy Zone Protein/PZP ELISA Kit PicoKine®Human PZP PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-trem-1-picokine-trade-elisa-kit-ek0844-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0844.pngHuman TREM-1 ELISA Kit PicoKine®Human TREM-1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cadherin-17-picokine-trade-elisa-kit-ek1998-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1998.jpgMouse LI-Cadherin-17 CDH17 ELISA Kit PicoKine®Mouse Cadherin-17 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-slpi-picokine-trade-elisa-kit-ek1996-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1996.pngMouse SLPI ELISA Kit PicoKine®Mouse SLPI PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-psg1-picokine-trade-elisa-kit-ek1997-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1997.jpgHuman PSG1 ELISA Kit PicoKine®Human PSG1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-nephrin-picokine-trade-elisa-kit-ek1999-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1999.pngHuman Nephrin ELISA Kit PicoKine®Human Nephrin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-il-17f-picokine-trade-elisa-kit-ek0795-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0795.pngHuman IL-17F/Interleukin-17F ELISA Kit PicoKine®Human IL-17F PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/products/primary-antibodies/anti-fabp2-i-fabp-picoband-trade-antibody-a02378-1-boster.html2026-03-24T05:21:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02378-1-fabp2-primary-antibodies-elisa-testing-1.jpgAnti-FABP2/I-FABP Antibody Sandwich ELISA - Recombinant mouse FABP2/I-FABP protein standard curve.<br>
Use in combination with reagents from Mouse FABP2/I-FABP ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1622).
https://www.bosterbio.com/products/primary-antibodies/anti-nap2-picoband-trade-antibody-a02736-1-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-NAP2/Ppbp AntibodyBoster Kit Box
https://www.bosterbio.com/products/primary-antibodies/anti-gsta1-gsta2-gsta3-gsta4-gsta5-antibody-picoband-a01462-2-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 AntibodyBoster Kit Box
https://www.bosterbio.com/products/primary-antibodies/anti-fgf21-picoband-trade-antibody-a00802-1-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00802-1-fgf21-primary-antibodies-elisa-testing-1.jpgAnti-FGF21 Antibody Sandwich ELISA - Recombinant human FGF21 protein standard curve.<br>
Use in combination with reagents from Human FGF21 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0994).
https://www.bosterbio.com/products/primary-antibodies/anti-he4-picoband-trade-antibody-a02685-5-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02685-5-he4-primary-antibodies-elisa-testing-1.jpgAnti-HE4/Wfdc2 Antibody Sandwich ELISA - Recombinant Rat HE4 protein standard curve.<br>
Use in combination with reagents from Rat HE4 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1624).
https://www.bosterbio.com/products/primary-antibodies/anti-lipocalin-2-ngal-picoband-trade-antibody-a00452-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00452-lipocalin-2-primary-antibodies-elisa-testing-1.jpgAnti-Lipocalin 2/LCN2 Antibody Sandwich ELISA - Recombinant mouse Lipocalin 2 protein standard curve.<br>
Use in combination with reagents from Mouse Lipocalin 2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0854).
https://www.bosterbio.com/products/primary-antibodies/anti-tnfrsf14-hvem-picoband-trade-antibody-a02298-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02298-tnfrsf14-primary-antibodies-elisa-testing-1.jpgAnti-TNFRSF14 Antibody Sandwich ELISA - Recombinant human TNFRSF14/HVEM protein standard curve.<br>
Use in combination with reagents from Human TNFRSF14/HVEM ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1226).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apobec3g-picoband-trade-antibody-monoclonal-m00708-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-wb-testing-1.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (M00708). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: Raji whole cell lysates, <br>
Lane 2: K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APOBEC3G antigen affinity purified monoclonal antibody (Catalog # M00708) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 46KD. The expected band size for APOBEC3G is at 46KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-ihc-testing-2.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708). <br>
APOBEC3G was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-ihc-testing-3.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708). <br>
APOBEC3G was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-ihc-testing-4.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) IHC analysis of APOBEC3G using anti-APOBEC3G antibody (M00708). <br>
APOBEC3G was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-fcm-testing-5.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) Flow Cytometry analysis of THP-1 cells using anti-APOBEC3G antibody (M00708).<br>Overlay histogram showing THP-1 cells stained with M00708 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-APOBEC3G Antibody (M00708,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00708-apobec3g-primary-antibodies-if-testing-6.jpgAnti-APOBEC3G Antibody Picoband® (monoclonal, 6C2) IF analysis of APOBEC3G using anti-APOBEC3G antibody (M00708). <br>
APOBEC3G was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-APOBEC3G Antibody (M00708) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-cyclophilin-b-picoband-trade-antibody-monoclonal-m03229-1-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-wb-testing-1.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: U-87MG whole cell lysates, <br>
Lane 3: HepG2 whole cell lysates, <br>
Lane 4: Caco-2 whole cell lysates, <br>
Lane 5: SW620 whole cell lysates, <br>
Lane 6: PANC-1 whole cell lysates, <br>
Lane 7: THP-1 whole cell lysates, <br>
Lane 8: HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin B antigen affinity purified monoclonal antibody (Catalog # M03229-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 21KD. The expected band size for Cyclophilin B is at 21KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-wb-testing-2.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) Western blot analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse spleen tissue lysates, <br>
Lane 4: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin B antigen affinity purified monoclonal antibody (Catalog # M03229-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclophilin B at approximately 21KD. The expected band size for Cyclophilin B is at 21KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-ihc-testing-3.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-ihc-testing-4.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-ihc-testing-5.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-ihc-testing-6.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-ihc-testing-7.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-if-testing-8.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (M03229-1). <br>
Cyclophilin B was detected in immunocytochemical section of U20S cell. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The cells were blocked with 10% goat serum. And then incubated with 2μg/ml mouse anti-Cyclophilin B Antibody (M03229-1) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Cy3 Conjugated Avidin (BA1037). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03229-1-cyclophilin_b-primary-antibodies-fcm-testing-9.jpgAnti-Cyclophilin B PPIB Antibody Picoband® (monoclonal, 11C11) Flow Cytometry analysis of U20S cells using anti-Cyclophilin B antibody (M03229-1).<br>Overlay histogram showing U20S cells stained with M03229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin B Antibody (M03229-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp-citrate-lyase-picoband-trade-antibody-monoclonal-m02372-1-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-wb-testing-1.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) Western blot analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: U-87MG whole cell lysates, <br>
Lane 3: HEK293 whole cell lysates, <br>
Lane 4: Caco-2 whole cell lysates, <br>
Lane 5: HL-60 whole cell lysates, <br>
Lane 6: Raji whole cell lysates, <br>
Lane 7: THP-1 whole cell lysates, <br>
Lane 8: PANC-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ATP citrate lyase antigen affinity purified monoclonal antibody (Catalog # M02372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ATP citrate lyase at approximately 121KD. The expected band size for ATP citrate lyase is at 121KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-wb-testing-2.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) Western blot analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates, <br>
Lane 2: rat testicular tissue lysates, <br>
Lane 3: rat kidney tissue lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: mouse lung tissue lysates, <br>
Lane 6: mouse testicular tissue lysates, <br>
Lane 7: mouse kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ATP citrate lyase antigen affinity purified monoclonal antibody (Catalog # M02372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ATP citrate lyase at approximately 121KD. The expected band size for ATP citrate lyase is at 121KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-ihc-testing-3.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) IHC analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
ATP citrate lyase was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP citrate lyase Antibody (M02372-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-ihc-testing-4.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) IHC analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
ATP citrate lyase was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP citrate lyase Antibody (M02372-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-ihc-testing-5.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) IHC analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
ATP citrate lyase was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP citrate lyase Antibody (M02372-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-ihc-testing-6.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) IHC analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
ATP citrate lyase was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP citrate lyase Antibody (M02372-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp_citrate_lyase-primary-antibodies-fcm-testing-7.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) Flow Cytometry analysis of A549 cells using anti-ATP citrate lyase antibody (M02372-1).<br>Overlay histogram showing A549 cells stained with M02372-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ATP citrate lyase Antibody (M02372-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-1-atp-citrate-lyase-primary-antibodies-if-testing-8.jpgAnti-ATP citrate lyase ACLY Antibody Picoband® (monoclonal, 5I2) IF analysis of ATP citrate lyase using anti-ATP citrate lyase antibody (M02372-1). <br>
ATP citrate lyase was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ATP citrate lyase Antibody (M02372-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-iii-col3a1-picoband-trade-antibody-monoclonal-m00788-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: Hela whole cell lysates, <br>
Lane 3: rat heart tissue lysates, <br>
Lane 4: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Collagen III/COL3A1 antigen affinity purified monoclonal antibody (Catalog # M00788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 180-190KD. The expected band size for Collagen III/COL3A1 is at 180-190KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-13659_2024_446_fig2_html.pngAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9)Scutellarin Improved Renal Fibrosis of the DN Mice. a Masson’s trichrome staining (× 200; scale bar = 50 µm) and immunohistochemistry staining for α-SMA and FN1 (× 200; scale bar = 100 µm) of the mice treated with vehicle, scutellarin or empagliflozin. b Representative western-blot images for α-SMA and Col3A1 of the mice. c , d Quantifications of the protein levels of α-SMA and Col3A1, respectively. β-Actin used as a loading control. All data are normalized to the STZ group and presented as the mean ± S.D.; n = 6 for each group, “n” stands for the number of animals; p vs. the model group (STZ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s13659-024-00446-y'>38656633</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-3.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-4.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-5.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of human lung cancerr tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-6.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-collagen_iiicol3a1-primary-antibodies-ihc-testing-7.jpgAnti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788). <br>
Collagen III/COL3A1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aif-picoband-trade-antibody-monoclonal-m01571-1-boster.html2026-03-16T09:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-wb-testing-1.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) Western blot analysis of AIF using anti-AIF antibody (M01571-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: A549 whole cell lysates, <br>
Lane 2: Raji whole cell lysates, <br>
Lane 3: PC-3 whole cell lysates, <br>
Lane 4: Hela whole cell lysates, <br>
Lane 5: Caco-2 whole cell lysates, <br>
Lane 6: HepG2 whole cell lysates, <br>
Lane 7: THP-1 whole cell lysates, <br>
Lane 8: PANC-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AIF antigen affinity purified monoclonal antibody (Catalog # M01571-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AIF at approximately 70KD. The expected band size for AIF is at 70KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-41419_2022_5279_fig3_html.pngAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5)HOXA5 overexpression attenuates the malignant behaviors of ECCA cells. A , B RT-qPCR and Western blot analyses validated HOXA5 overexpression, which reduced the mitosis-related gene expression in ECCA cells. HOXA5 overexpression did not alter the EMT-related gene expression, but increased AIFM1, and decreased BCL-2 expression in ECCA cells. C HOXA5 overexpression induced cell cycle arrest in G1/G0 phase. D , E HOXA5 overexpression inhibited the proliferation of ECCA cells. F HOXA5 overexpression decreased the tumor volumes in mice. G Immunohistochemistry analysis of HOXA5 and Ki67 expression in xenograft tumors from nude mice. Data are representative images from three separate experiments. * P < 0.05. ** P < 0.01. *** P < 0.001. Orange bar, 50 μm. Green bar, 20 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05279-6'>36167790</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-41419_2022_5279_fig5_html.pngAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5)MXD1 overexpression reduces the malignant behaviors of ECCA cells. A , B RT-qPCR and Western blot validated MXD1 overexpression in ECCA cells. B Western blot analysis of the expression of mitosis-related genes in ECCA cells. MXD1 overexpression failed to modulate not only the EMT-related gene expression, but also the expression of AIFM1 and BCL-2 in ECCA cells. C – E MXD1 overexpression attenuated the cell cycle C , proliferation D , E of ECCA cells. F MXD1 overexpression decreased the tumor volumes in mice. G Immunohistochemistry analysis of MXD1 and Ki67 expression in the xenograft tumors from nude mice. Data are representative images of each group from three separate experiments. * P < 0.05. ** P < 0.01. *** P < 0.001. Orange bar, 50 μm. Green bar, 20 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05279-6'>36167790</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-wb-testing-2_1.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) Western blot analysis of AIF using anti-AIF antibody (M01571-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat kidney tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse spleen lysates, <br>
Lane 4: mouse testicular tissue lysates, <br>
Lane 5: mouse kidney tissue lysates, <br>
Lane 6: RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AIF antigen affinity purified monoclonal antibody (Catalog # M01571-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AIF at approximately 70KD. The expected band size for AIF is at 70KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-ihc-testing-3.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) IHC analysis of AIF using anti-AIF antibody (M01571-1). <br>
AIF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-AIF Antibody (M01571-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-ihc-testing-4.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) IHC analysis of AIF using anti-AIF antibody (M01571-1). <br>
AIF was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-AIF Antibody (M01571-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-ihc-testing-5.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) IHC analysis of AIF using anti-AIF antibody (M01571-1). <br>
AIF was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-AIF Antibody (M01571-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-if-testing-8.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) IF analysis of AIF using anti-AIF antibody (M01571-1). <br>
AIF was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-AIF Antibody (M01571-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-if-testing-6.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) IF analysis of AIF using anti-AIF antibody (M01571-1). <br>
AIF was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-AIF Antibody (M01571-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-1-aif-primary-antibodies-fcm-testing-7.jpgAnti-AIF/AIFM1 Antibody Picoband® (monoclonal, 2I5) Flow Cytometry analysis of Raji cells using anti-AIF antibody (M01571-1).<br>Overlay histogram showing Raji cells stained with M01571-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AIF Antibody (M01571-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmi-picoband-trade-antibody-monoclonal-m02768-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-wb-testing-1.jpgAnti-NMI Antibody Picoband® (monoclonal, 2F3) Western blot analysis of NMI using anti-NMI antibody (M02768). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: K562 whole cell lysates, <br>
Lane 3: Hela whole cell lysates, <br>
Lane 4: A431 whole cell lysates, <br>
Lane 5: PC-3 whole cell lysates, <br>
Lane 6: Caco-2 whole cell lysates, <br>
Lane 7: HEK293 whole cell lysates, <br>
Lane 8: mouse intestine tissue lysates, <br>
Lane 9: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NMI antigen affinity purified monoclonal antibody (Catalog # M02768) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NMI at approximately 40KD. The expected band size for NMI is at 35KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-ihc-testing-2.jpgAnti-NMI Antibody Picoband® (monoclonal, 2F3) IHC analysis of NMI using anti-NMI antibody (M02768). <br>
NMI was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-ihc-testing-3.jpgAnti-NMI Antibody Picoband® (monoclonal, 2F3) IHC analysis of NMI using anti-NMI antibody (M02768). <br>
NMI was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-ihc-testing-4.jpgAnti-NMI Antibody Picoband® (monoclonal, 2F3) IHC analysis of NMI using anti-NMI antibody (M02768). <br>
NMI was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NMI Antibody (M02768) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-if-testing-5.jpgAnti-NMI Antibody Picoband® (monoclonal, 2F3) IF analysis of NMI using anti-NMI antibody (M02768). <br>
NMI was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-NMI Antibody (M02768) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-nmi-primary-antibodies-fcm-testing-6.pngAnti-NMI Antibody Picoband® (monoclonal, 2F3) Flow Cytometry analysis of A431 cells using anti- NMI antibody (M02768). <br>Overlay histogram showing A431 cells stained with M02768 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NMI Antibody (M02768, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gm130-picoband-trade-antibody-monoclonal-m05865-2-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-wb-testing-1.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) Western blot analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: Hela whole cell lysates, <br>
Lane 3: K562 whole cell lysates, <br>
Lane 4: Caco-2 whole cell lysates, <br>
Lane 5: A549 whole cell lysates, <br>
Lane 6: A431 whole cell lysates, <br>
Lane 7: HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GM130 antigen affinity purified monoclonal antibody (Catalog # M05865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130KD. The expected band size for GM130 is at 130KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-41531_2025_902_fig4_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)A glycosylation defect in GCase leads to significant retention in the Golgi apparatus, which subsequently results in LPC-induced abnormal accumulation of GlcCer. a , b Western blot experiments assessing the expression level of non-glycosylated GCase in MES23.5 dopaminergic neurons after treatment with glycosidic endonuclease Endo-H ( n = 3, Control vs Endo-H, t = 7.996, p = 0.0013). c , d Western blot analyses of non-glycosylated GCase expression in MES23.5 dopaminergic neurons following intervention with different doses of LPC ( n = 3, Control vs LPC5: p = 0.4962, ns, Control vs LPC10: p < 0.0001, Control vs LPC15: p = 0.0004). e , f Glycosylated/non-glycosylated GCase ratio in MES23.5 dopaminergic neurons analyzed via Western blotting after LPC treatment, CBE intervention, and ML198 administration ( n = 3, Control vs LPC: p < 0.0001, Control vs CBE: p < 0.0001, LPC vs LPC + ML198: p = 0.0030). g Immunofluorescence co-labeling images of GCase and GM130 in MES23.5 dopaminergic neurons post-LPC intervention. h – j Statistical analysis of changes in the co-labeling degree of GCase and GM130 ( n = 3, t = 4.331, p = 0.0123). k Immunofluorescence co-labeling image of GCase with LAMP1 in MES23.5 dopaminergic neurons after LPC intervention. l – n Statistical analysis of changes in the co-labeling degree of GCase and LAMP1 ( n = 3, t = 11.30, p = 0.0003). O Heatmap illustrating alterations in the abundance of the glucosylceramide family in MES23.5 dopaminergic neurons following LPC intervention analyzed by high-performance liquid chromatography-mass spectrometry. p – s Western blot analyses were conducted to assess α-Synuclein expression in MES23.5 dopaminergic neurons following modulation of glucosylceramide levels. ( n = 3, oligomers: Control vs LPC: p = 0.0004, Control vs CBE: p = 0.00002, Control vs ML198 + LPC: p = 0.0033; monomer: Control vs LPC: p = 0.0002, Control vs CBE: p < 0.00001, Control vs ML198 + LPC: p = 0.0023; oligomers/ monomer: Control vs LPC: p = 0.0052, Control vs CBE: p = 0.0007, LPC vs ML198 + LPC: p = 0.0428). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41531-025-00902-7'>40089519</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-41531_2025_902_fig6_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)GRASP65-mediated Golgi structural disruption by LPC leads to impaired glycosylation of GCase. a , b Western blot experiments assessing GRASP65 expression levels in MES23.5 dopaminergic neurons transfected with GRASP65 overexpression versus knockdown virus ( n = 3, Control vs GRASP65 OE: p = 0.0004, Control vs GRASP65 KD: p = 0.0020). c Fluorescence staining images depicting MES23.5 dopaminergic neurons transfected with GRASP65 overexpression versus knockdown virus and labeled with GM130 following LPC intervention. d Quantification of GM130 positive area ( n = 3, Control vs LPC: p = 0.0068, Control vs GRASP65 KD: p = 0.0073, Control vs LPC + GRASP65 OE: p > 0.9999, ns). e Transmission electron microscopic observation of morphological changes in the Golgi apparatus after LPC intervention in MES23.5 dopaminergic neurons. f , g Western blot analyses of glycosylate/non-glycosylated GCase expression in MES23.5 dopaminergic neurons following transfection with GRASP65 overexpression versus knockdown virus and LPC intervention ( n = 3, Control vs LPC: p < 0.0001, Control vs. GRASP65 KD: p < 0.0001, LPC vs LPC + GRASP65 OE: p < 0.0001). h Fluorescence staining images showing labeled α-Syn on MES23.5 dopaminergic neurons transfected with GRASP65 overexpression versus knockdown virus followed by LPC intervention. i Quantification of α-Syn fluorescence intensity (n = 3, Control vs. LPC: p < 0.0001, Control vs. GRASP65 KD: p = 0.0002, LPC vs. LPC + GRASP65 OE: p < 0.0001). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41531-025-00902-7'>40089519</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig1_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)After HSV-1 infection, change in microglial morphology and GM130, TLR3, and inflammatory cytokines. a Cell morphology and statistical results of BV2 cells at 6, 12, and 24 h after Mock and HSV-1 infection. HSV-1-infected BV2 cells with MOI = 1 and observed cell morphology under an inverted microscope (amoeba morphology is shown by a black arrow). The cell morphology statistics at each time point are obtained from three different visual fields, with a scale of 50 μm. b One-step growth curve of HSV-1 in BV2 cells. HSV-1 infection infects BV2 cells with MOI = 1. c , d Protein levels of GM130, TLR3, and HSV-1 are analyzed semiquantitatively by scanning a grayscale, and βactin is used as internal parameter correction. The expression levels of GM130, TLR3, and HSV-1 are homogenized at each time point compared to those of the uninfected group. Semiquantitative results of e are shown in f and g . h The secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 in the medium are detected by ELISA. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. ns, *, **, *** represent other groups vs. Mock group; ns, #, ##, ##, and ## represent the comparison between the two groups marked with a short horizontal line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-41531_2025_902_fig7_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)LPC activates caspase-3 via the GPR35-ERK signaling pathway, subsequently leading to the cleavage of GRASP65 and the consequent disruption of Golgi structure. a Volcano plot illustrating the top 50 differentially expressed mRNAs following LPC modeling, highlighting up- and down-regulated transcripts. b Volcano plots delineating the top 20 differentially expressed mRNAs identified through analysis of the GEO database GSE213100, indicating both up- and down-regulated genes. c Venn diagrams displaying the intersecting up- and down-regulated genes derived from both LPC modeling and GEO database analysis. d Heatmap visualizing the expression patterns of intersecting up-regulated genes. e Schematic representation illustrating the docking results of LPC and GPR35. f Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses conducted on the differentially expressed genes. g – i Western blot analyses of p-ERK expression levels following intervention with LPC, GPR35 agonist, and antagonist (n = 3, Control vs LPC: p = 0.0025, Control vs Pamoic acid: p = 0.0029, LPC vs LPC + CID2745687: p = 0.0029), and Cleaved-Caspase3 expression levels ( n = 3, Control vs. LPC: p < 0.0001, Control vs Pamoic acid: p < 0.0001, LPC vs LPC + CID2745687: p < 0.0001). j – l Western blot analyses of Cleaved-Caspase3 and GRASP65 expression levels post-intervention with LPC, Caspase3 agonist, and antagonist ( n = 3, Cleaved-Caspase3: Control vs LPC: p = 0.0016, Control vs Raptinal: p = 0.0431, LPC vs LPC + Z-DEVD-FMK: p = 0.0001, GRASP65: Control vs LPC: p < 0.0001, Control vs Raptinal: p < 0.0001, LPC vs LPC + Z-DEVD-FMK: p = 0.0252). m Immunofluorescence co-labeling plots depicting Cleaved-Caspase3 with GM130 post-LPC intervention. n Statistical correlation analysis of Cleaved-Caspase3 and Golgi area ( n = 12, r = 0.7271, p < 0.0001). 0 Statistical assessment of the co-localization degree of Cleaved-Caspase3 with GM130 ( n = 3, t = 4.431, p = 0.0114). p Quantification of GM130 positive area ( n = 3, t = 4.530, p = 0.0106). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41531-025-00902-7'>40089519</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig2_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Morphological changes of the Golgi apparatus (GA) at different time points after microglia infection with HSV-1. a Fluorescence intensity and GA structure of GM130 are observed by immunofluorescence, and IBA1 is used to label microglia cells. Re-stained the nucleus with DAPI (blue channel). Merge the above channels into the same field of view to produce a merged image. Scale, 10 μm. b Since the expression of P115 remained unchanged after infection with HSV-1 in BV2 cells, further fluorescence labeling of P115 is performed to observe the structural changes of GA. Re-stained the nucleus with DAPI (blue channel). Scale, 10 μm. In the infected group at different time points, the fluorescently labeled GA structure gradually became loose, from tightly stacked perinuclear to loosely distributed in the cytoplasm (white arrow). The representative images are obtained from three independently repeated experiments. Scale, 10 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig3_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Changes in knockdown GM130 in uninfected and HSV-1-infected BV2 cells. a , d Knockdown GM130 without infection and semiquantitative results of knockdown GM130 without infection. b , e Knockdown GM130 12 h after infection and semi-quantitative results of knockdown GM130 12 h after infection. c Effect of siRNA knockdown GM130 on viral replication. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. *, **, and *** represent the comparison with the NC group. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig4_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Structural changes in the Golgi apparatus (GA) after GM130 knockdown in microglia cells. a After GM130 knockdown in the uninfected and 12 h infected groups, the GA structure (GM130 marker) is observed by immunofluorescence, and IBA1 is used to label microglia cells. The GA fragmentation is shown in IF (white arrow). Immunostaining is performed for GM130 (green channel) and IBA1 (red channel) of transfected cells, and DAPI (blue channel) re-stained the nucleus. Scale, 10 μm. b The GA structure is observed by immunostaining for P115 (red channel) of transfected cells and DAPI (blue channel) re-stained nucleus. Scale, 10 μm. c Transmission electron microscopy images of the 12 h infection and GM130 knockdown groups showed GA disruption and swelling of the vesicle structure, from tightly packed (black arrow) to disordered swelling (red arrow). Quantification of Golgi cisternae lumen (µm) in mock- and HSV-1-infected cells and cells transfected with SiGM130. Data represent the average maximum luminal width of the Golgi cisternae from three independent experiments. Data are shown as mean ± SD and were analyzed using the Student’s t-test. Representative electron micrographs of the Golgi cisternae in mock- and HSV-1-infected cells and cells transfected with SiGM130. Representative images are obtained from three independent repeated experiments with scales of 1 μm and 0.5 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig5_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Changes in overexpression of GM130 in uninfected and HSV-1-infected BV2 cells. a , d Overexpress GM130 without infection and semiquantitative results of knockdown GM130 without infection. b , e Overexpress GM130 12 h after infection and semiquantitative results of overexpress GM130 12 h after infection. c Effect of Plasmid overexpress GM130 on viral replication. Data were obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. *, **, *** represent the comparison with the NC group. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig6_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Structural changes in the Golgi apparatus (GA) after GM130 overexpression in microglia cells. a 12 h was selected as the infection time point; 36 h after plasmid transfection, HSV-1 infected cells with MOI = 1, and 12 h after infection, GA structure (GM130 marker) is observed. Scale, 10 μm. b HSV-1 infected cells with MOI = 1 at 36 h after plasmid transfection, and GA structure (P115 label) is observed by immunofluorescence at 12 h after infection. Scale, 10 μm. The GM130 and P115 labeled GA structures gradually loosen 12 h after infection, from tightly stacked perinuclear to loosely distributed in the cytoplasm (white arrow). Fluorescently labeled GA structures after overexpression of GM130 partially regain a compact perinuclear distribution (red arrow). The representative images are obtained from three independently repeated experiments. Scale, 10 μm. c Transmission electron microscope images after overexpression of GM130 showed that GA improved the swelling and disintegration of GA caused by HSV-1 infection (red arrow) and reshaped the structure of GA (black arrow). d Quantification of Golgi cisternae lumen (µm) in group HSV-1 + NC and group HSV-1 + OEGM130. The representative images are obtained from three independently repeated experiments. Scale 1 μm, 0.5 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig7_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Effects of Poly (I:C) treatment of GM130 knockdown BV2 cells on TLR3 and inflammatory cytokines. a Protein levels of GM130, TLR3, and HSV-1 are treated with Poly (I:C), and βactin is used as the internal parameter correction. Protein levels in each group are homogenized compared to those of the uninfected group. b Semi-quantitative results of GM130. c Semi-quantitative results of TLR3. d Semi-quantitative results of HSV-1 gD. e Secretion levels of inflammatory cytokines IFN-β, TNF-α, and IL-6 after Poly (I:C) treatment. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig8_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Effects of TLR3i treatment of GM130 overexpressed BV2 cells on TLR3 and inflammatory cytokines. a Protein levels of GM130, TLR3, and HSV-1 after TLR3i treatment were adjusted with βactin as internal reference. The expression levels of the detected proteins in each group were compared with those of the non-infected group. b Semi-quantitative results of GM130. c Semi-quantitative results of TLR3. d Semi-quantitative results of HSV-1 gD. e Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with (TLR3i). Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig9_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Effect of berberine (BBR) combined with acyclovir (ACV) on GM130 on TLR3 and inflammatory cytokines. a Cellular activity of cells treated with different concentrations of BBR for 24 h. Selection of BBR at a concentration of 3 μM pretreated for 12 h followed by HSV-1 infection for 12 h, and collection of samples. Cells are pretreated with ACV (3 μM) for 12 h and infected with HSV-1 for 12 h. b Protein levels of GM130, TLR3, and HSV-1 treated with BBR and ACV were adjusted with βactin as the internal reference. The expression levels of the detected proteins in each group were compared with those of the uninfected group. c Semi-quantitative results of GM130. d Semi-quantitative results of TLR3. e Semi-quantitative results of HSV-1-gD. f – h Secretion levels of the inflammatory cytokines IFN-β, TNF-α, and IL-6 after treatment with BBR and ACV. i Viral titers are treated with BBR and ACV. Data are obtained through three independent replicates, expressed as X ± SD, and statistically analyzed between the two groups using a t-test. A comparison between the two groups is marked with a dashed line. ns, p > 0.05; *, #, p < 0.05; **, ##, p < 0.01; ***, ###, p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-12985_2024_2492_fig10_html.pngAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4)Structural changes of the Golgi apparatus (GA) in microglia after treatment with acyclovir (ACV) and berberine (BBR). ACV and BBR were pretreated for 12 h before infection, HSV-1 infected cells with MOI = 1, and GA structure (GM130 marker, P115 marker) is observed 12 h after infection. Scale, 10 μm. After ACV treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After BBR treatment, the fluorescently labeled GA structure of GM130 and P115 partially recovered compact perinuclear distribution. After ACV is combined with BBR, the fluorescently labeled GA structure of GM130 and P115 is further restored to a compact perinuclear distribution. The representative images are obtained from three independent repeated experiments. Scale, 10 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12985-024-02492-x'>39285274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-wb-testing-2.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) Western blot analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse brain tissue lysates, <br>
Lane 4: mouse liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GM130 antigen affinity purified monoclonal antibody (Catalog # M05865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130KD. The expected band size for GM130 is at 130KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-ihc-testing-3.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) IHC analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
GM130 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GM130 Antibody (M05865-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-ihc-testing-4.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) IHC analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
GM130 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GM130 Antibody (M05865-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-ihc-testing-5.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) IHC analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
GM130 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GM130 Antibody (M05865-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-if-testing-6.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) IF analysis of GM130 using anti-GM130 antibody (M05865-2). <br>
GM130 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-GM130 Antibody (M05865-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-2-gm130-primary-antibodies-fcm-testing-7_1.jpgAnti-GM130 GOLGA2 Antibody Picoband® (monoclonal, 6D4) Flow Cytometry analysis of A431 cells using anti-GM130 antibody (M05865-2).<br>
Overlay histogram showing A431 cells stained with M05865-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GM130 Antibody (M05865-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e-cadherin-picoband-trade-antibody-monoclonal-m00063-2-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-1_1.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) Western blot analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-E Cadherin antigen affinity purified polyclonal antibody (Catalog # M00063-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for E Cadherin at approximately 130KD. The expected band size for E Cadherin is at 97KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-12885_2019_6225_fig1_html.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)Expression of RVG, NDV, α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal markers proteins in infected BGC and SGC cells. a Western blot analysis of RVG, NDV, α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal proteins. b Immunofluorescence analysis of P-ERK. c Immunofluorescence analysis of EMT protein markers E-cadherin. BGC and SGC cells were infected with either rL-RVG, NDV and PBS for 24 h. *P < 0.5, **P < 0.01.(rL-RVG vs NDV,rL-RVG vs NDV and PBS groups, respectively, Bar = 25 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-019-6225-9'>31640627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-12885_2019_6225_fig3_html.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)Effects of rL-RVG and ACB pretreated SGC cells on the α7-nAChR, MEK/ERK signaling pathway, epithelial/mesenchymal proteins and cell migration. a Western blot analysis of α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal protein marker. b Cell migration was detected by wound healing and transwell assay. c Immunofluorescence analysis of P-ERK. d Immunofluorescence analysis of EMT proteins E-cadherin in infected SGC cells. *P < 0.5 ,**P < 0.01.(rL-RVG + ACB vs rL-RVG, NDV + ACB vs NDV, PBS + ACB vs PBS, respectively, Bar = 25 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-019-6225-9'>31640627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-12885_2019_6225_fig4_html.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)Effects of rL-RVG and MLA pretreated cells on α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal proteins and migration of cells. a Western blot analysis of the α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal protein markers. b Cell migration was detected by wound healing and transwell assay. c Immunofluorescence analysis of P-ERK. d Immunofluorescence analysis of EMT markers E-cadherin in infected SGC cells. *P < 0.5 ,**P < 0.01 (rL-RVG + MLA vs rL-RVG, NDV + MLA vs NDV, PBS + MLA vs PBS,Bar = 25 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-019-6225-9'>31640627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-12885_2019_6225_fig5_html.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)Effects of rL-RVG and si-RNA pretreated SGC cells on the α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal proteins and cell migration. a Western blot analysis of α7-nAChR, MEK/ERK signaling pathway and epithelial/mesenchymal proteins. b Cell migration was detected by wound healing and transwell assay. c Immunofluorescence analysis of P-ERK. d Immunofluorescence analysis of EMT proteins E-cadherin in infected SGC cells. *P < 0.5 ,**P < 0.01 (rL-RVG + si-RNA vs rL-RVG,NDV + si-RNA vs NDV, PBS + si-RNA vs PBS,Bar = 25 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-019-6225-9'>31640627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-12885_2019_6225_fig6_html.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2)Effects of rL-RVG and corynoxeine pretreated SGC cells on α7-nAChR, ERK and epithelial/mesenchymal proteins and cell migration. a Western blot analysis of α7-nAChR, P-ERK/ERK and epithelial/mesenchymal markers. b Cell migration of SGC cells was detected by wound healing and transwell assay. c Immunofluorescence analysis of P-ERK in SGC cells. d Immunofluorescence analysis of EMT protein E-cadherin in infected SGC cells. *P < 0.5 ,**P < 0.01 (rL-RVG + corynoxeine vs rL-RVG, NDV + corynoxeine vs NDV, PBS + corynoxeine vs PBS,Bar = 25 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-019-6225-9'>31640627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-e-cadherin-primary-antibodies-ihc-testing-2.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E Cadherin was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (M00063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-e-cadherin-primary-antibodies-ihc-testing-3.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E Cadherin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (M00063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-e-cadherin-primary-antibodies-ihc-testing-4.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E Cadherin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (M00063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-e-cadherin-primary-antibodies-ihc-testing-5_1.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IHC analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E Cadherin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-E Cadherin Antibody (M00063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-6.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) <br> COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031),were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-7.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IF analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E Cadherin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-E Cadherin Antibody (M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-8.jpgAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) IF analysis of E Cadherin using anti-E Cadherin antibody (M00063-2). <br>
E-cadherin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-E Cadherin Antibody (M00063-2) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00063-2-e-cadherin-primary-antibodies-fcm-testing-9.pngAnti-E Cadherin 1 CDH1 Antibody Picoband® (monoclonal, 9G2) Flow Cytometry analysis of A549 cells using anti- CA2 antibody (M00063-2). <br>Overlay histogram showing A549 cells stained with M00063-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CA2 Antibody (M00063-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat1-picoband-trade-antibody-monoclonal-m00036-2-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-wjg-13-4080-g002.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7)Tyr701 phosphorylated STAT1 in Huh7 cells induced by IFNα-2b (10 000 U/mL) (Western blot).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4205308/&orig_host=www.ncbi.nlm.nih.gov'>17696225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-wjg-13-4080-g003.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7)STAT1 or Phosphorylated STAT1 (Tyr701) in Huh7 cells stimulated by IFNα-2b (10 000 U/mL) for 30 min (FITC, × 400). A: STAT1; B: Tyr701.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4205308/&orig_host=www.ncbi.nlm.nih.gov'>17696225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-wjg-13-4080-g005.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7)Phosphorylated STAT1 from IFN induced Huh7 cells (FITC, × 400): A: P CNS5A -transfected; B: P RC/CMV -transfected; C: Non-transfected.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4205308/&orig_host=www.ncbi.nlm.nih.gov'>17696225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-wb-testing-1_1.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) Western blot analysis of STAT1 using anti-STAT1 antibody (M00036-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human placenta tissue lysates,<br>
Lane 3: human 22RV1 whole cell lysates.<br>
Lane 4: monkey COS-7 whole cell lysates,<br>
Lane 5: human SW620 whole cell lysates,<br>
Lane 6: human HepG2 whole cell lysates,<br>
Lane 7: human PANC-1 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STAT1 antigen affinity purified monoclonal antibody (Catalog # M00036-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-wjg-13-4080-g004.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7)STAT1 or phosphorylated STAT1 in cytoplasm and nuclei of Huh7 cells after 30 min IFN induced (FITC, × 400): A and D: P CNS5A -transfected; B and E: P RC/CMV -transfected; C and F: non-transfected.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4205308/&orig_host=www.ncbi.nlm.nih.gov'>17696225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-ihc-testing-2.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of STAT1 using anti STAT1 antibody (M00036-2). <br>
STAT1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STAT1 Antibody (M00036-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-ihc-testing-3.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of STAT1 using anti STAT1 antibody (M00036-2). <br>
STAT1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STAT1 Antibody (M00036-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-ihc-testing-4.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of STAT1 using anti STAT1 antibody (M00036-2). <br>
STAT1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STAT1 Antibody (M00036-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-ihc-testing-5.jpgAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) IHC analysis of STAT1 using anti STAT1 antibody (M00036-2). <br>
STAT1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-STAT1 Antibody (M00036-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-2-stat1-primary-antibodies-fcm-testing-6.pngAnti-STAT1 Antibody Picoband® (monoclonal, 12C7) Flow Cytometry analysis of A431 cells using anti- STAT1 antibody (M00036-2). <br>Overlay histogram showing A431 cells stained with M00036-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-STAT1 Antibody (M00036-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ednrb-picoband-trade-antibody-monoclonal-m01041-1-boster.html2026-03-24T05:21:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01041-1-ednrb-primary-antibodies-wb-testing-1.jpgAnti-EDNRB Antibody Picoband® (monoclonal, 15C3) Western blot analysis of EDNRB using anti-EDNRB antibody (M01041-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human SW620 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EDNRB antigen affinity purified monoclonal antibody (Catalog # M01041-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EDNRB at approximately 45KD. The expected band size for EDNRB is at 45KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a1-picoband-trade-antibody-monoclonal-m00163-1-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00163-1-slc2a1-primary-antibodies-wb-testing-1.jpgAnti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human placenta tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SLC2A1 antigen affinity purified monoclonal antibody (Catalog # M00163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00163-1-slc2a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) IHC analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1). <br>
SLC2A1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00163-1-slc2a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) IHC analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1). <br>
SLC2A1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00163-1-slc2a1-primary-antibodies-if-testing-4.jpgAnti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) IF analysis of SLC2A1 using anti-SLC2A1 antibody (M00163-1). <br>
SLC2A1 was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-SLC2A1 Antibody (M00163-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00163-1-slc2a1-primary-antibodies-fcm-testing-5_1.pngAnti-SLC2A1 Antibody Picoband® (monoclonal, 10C10) Flow Cytometry analysis of U20S cells using anti- SLC2A1 antibody (M00163-1). <br>Overlay histogram showing U20S cells stained with M00163-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SLC2A1 Antibody (M00163-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppt1-picoband-trade-antibody-monoclonal-m02690-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-ppt1-primary-antibodies-wb-testing-1.jpgAnti-PPT1 Antibody Picoband® (monoclonal, 10F3) Western blot analysis of PPT1 using anti-PPT1 antibody (M02690).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PPT1 antigen affinity purified monoclonal antibody (Catalog # M02690) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PPT1 at approximately 34KD. The expected band size for PPT1 is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-12935_2022_2508_fig1_html.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3)Role of PPT1 in HCC. A PPT1 mRNA expression in various normal human tissues and tumor tissues. B TCGA database-based comparison of PPT1 mRNA expression in HCC tissues (n = 371) and normal liver tissues (n = 50); *** P < 0.001. C Representative immunohistochemical results of PPT1 in patient-derived HCC tissue and normal liver tissue. Scale bar, 50 μm. D Expression of PPT1 in a panel of normal liver cell lines (L-O2 and MIHA) and HCC cell lines. On Western blot analysis, PPT1 was found to be preferentially expressed in HCC cell lines, Hep 3B and Hep 1-6. E Kaplan–Meier curves of overall survival (OS). High PPT1 expression was correlated with poor OS in HCC patients. F Cox proportional hazards regression model analysis of OS. G Gene set enrichment analysis (GSEA) using the TCGA dataset. PPT1 overexpression was significantly correlated with “KEGG_PATHWAYS_IN_CANCER”, “KEGG_APOPTOSIS”, “KEGG_REGULATION_OF_AUTOPHAGY”, and “KEGG_LYSOSOME” pathways. NES: normalized enrichmentscore <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-022-02508-y'>35277179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-12935_2022_2508_fig2_html.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3)Sorafenib induced autophagy and upregulated the expression of PPT1 in sorafenib-resistant HCC cells. A Western blot showing increase in LC3-II in HCC cells after treatment with sorafenib (10 µΜ, 24 h). B Photographs from transmission electron microscopy showing an increase in autophagosomes in HCC cells after treatment with sorafenib (10 µΜ, 24 h). Scale bar, 2 μm. C Fluorescence microscopic images showing punctate fluorescence from transfected mCherry-GFP-LC3 constructs in HCC cells treated with sorafenib (10 µΜ, 24 h); nuclei are labeled with Hoechst 33,258. Arrowheads indicate typical examples of co-localized particles of GFP and mCherry signal, while the arrow points to a typical example of a particle with an mCherry signal but without GFP signal. Scale bar, 10 μm. D IC50 values of sorafenib (48 h) for Hep 3B, Hep 3B-SR, Hep 1 – 6, and Hep 1-6-SR cells determined by CCK-8 assay. The data shown are from three independent experiments. E Confirmation of the upregulation of PPT1 protein in sorafenib-resistant cells derived from Hep 3B and Hep 1 – 6 by Western blot analysis <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-022-02508-y'>35277179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-12935_2022_2508_fig3_html.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3)PPT1 inhibitor DC661 inhibited autophagy by inhibiting lysosomes. A Confocal laser-scanning microscopy images of intracellular co-localization between PPT1 and lysosomes. LAMP-1 staining indicates the lysosomes, and Hoechst 33,258 indicates the nucleus. Co-localization is visualized by color and area overlap (red + green = yellow). Scale bar, 10 μm. B Fluorescence co-localization analysis according to ( A ) was performed by ImageJ software. C Immunofluorescence staining of PPT1 in HCC cells after treatment with DC661 (3 µM, 6 h). Scale bar, 20 μm. D Semiquantitative analysis of the mean fluorescence intensity (MFI) in the HCC cells according to ( C ) was performed by using ImageJ software (n = 6). Data represent mean ± SD; ** P < 0.01. E Fluorescence images of LysoTracker Green (lysosome probe) in HCC cells after treatment with DC661 (3 µM, 6 h). Hoechst 33,258 was used to stain the nucleus. Scale bar, 50 μm. F Semiquantitative analysis of the MFI of LysoTracker Green in HCC cells according to ( E ) was performed by ImageJ software (n = 6). Data represent mean ± SD; ** P < 0.01. G Western blot showing an increase in LC3-II and P62 in HCC cells treated with DC661 (3 µM, 6 h). H Western blot showing P62 degradation and LC3 lipidation in HCC cells treated with sorafenib and/or DC661. HCC cells were treated with or without 10 µM sorafenib in the presence or absence of 1 µM DC661 for 24 h <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-022-02508-y'>35277179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-12935_2022_2508_fig4_html.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3)Potential molecular mechanism of lysosomal membrane hyperpermeability induced by PPT1 inhibitor. A Fluorescence images of acridine orange staining in HCC cells treated with DC661 (3 µM, 6 h). Hoechst 33,258 staining marks the nuclei. Scale bar, 50 μm. B Gene correlation analysis using TCGA dataset. Heat map of the correlation between multiple genes and one gene. PPT1 expression was positively correlated with HSP70.1 and BMP expression in HCC (linear regression); ** P < 0.01. C Immunofluorescent staining of HSP70.1 in HCC cells treated with DC661 (3 µM, 6 h). Scale bar, 10 μm. D Semiquantitative analysis of the mean fluorescence intensity (MFI) in HCC cells according to ( C ) was performed by using ImageJ software (n = 6). Data represent mean ± SD; * P < 0.05. E Gene correlation analysis using TCGA dataset. PPT1 expression was positively correlated with HSF1 and calpain expression in HCC (linear regression); P < 0.001. F Gene correlation analysis using TCGA dataset. PPT1 expression was positively correlated with EP300 and mTOR expression in HCC (linear regression); P < 0.001. G Schematic illustration of a potential molecular mechanism of lysosomal membrane hyperpermeability induced by PPT1 inhibitor. HSE: heat shock element <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-022-02508-y'>35277179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-12935_2022_2508_fig6_html.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3)PPT1 inhibitor DC661-induced immunogenic cell death promoted the maturation of DCs and the activation of CD8 + T cells. A Immunofluorescent imaging of CRT expression on the cell surface of Hep 1-6 cells treated with DC661 (3 µM, 6 h). Nuclei are stained with Hoechst 33,258. Scale bars, 10 μm. B Immunofluorescent imaging of HMGB-1 release by Hep 1-6 cells treated with DC661 (3 µM, 6 h). Nuclei are stained with Hoechst 33,258. Scale bars, 10 μm. C ELISA detection of HMGB1 release into cell culture medium. Cells were incubated with DC661 at 3 µM for 6 h. Data represent mean ± SD; *** P < 0.001. D ELISA detection of ATP release into the cell culture medium. Cells were incubated with DC661 at 3 µM for 6 h. Data represent mean ± SD; *** P < 0.001. E , F Flow cytometric analysis (left) and quantification (right) of mature DC cells (CD11c + CD80 + CD86 + ) from the spleens of DC661- or vehicle-treated Hep 1-6 tumor-bearing mice. Data represent mean ± SD; *** P < 0.001. G–I The expression level of PPT1 was associated with the immune infiltration in the HCC tumor microenvironment. Scatter plots ( G ) and correlation diagrams ( H , I ) showing the difference of CD4 + T cells and macrophages infiltration level between PPT1-high and -low groups in TCGA-LIHC. ** P < 0.01; *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-022-02508-y'>35277179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-ppt1-primary-antibodies-ihc-testing-2.jpgAnti-PPT1 Antibody Picoband® (monoclonal, 10F3) IHC analysis of PPT1 using anti PPT1 antibody (M02690). <br>
PPT1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPT1 Antibody (M02690) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-ppt1-primary-antibodies-ihc-testing-3.jpgAnti-PPT1 Antibody Picoband® (monoclonal, 10F3) IHC analysis of PPT1 using anti PPT1 antibody (M02690). <br>
PPT1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPT1 Antibody (M02690) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-ppt1-primary-antibodies-ihc-testing-4.jpgAnti-PPT1 Antibody Picoband® (monoclonal, 10F3) IHC analysis of PPT1 using anti PPT1 antibody (M02690). <br>
PPT1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPT1 Antibody (M02690) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02690-ppt1-primary-antibodies-fcm-testing-5.pngAnti-PPT1 Antibody Picoband® (monoclonal, 10F3) Flow Cytometry analysis of THP-1 cells using anti- PPT1 antibody (M02690). <br>Overlay histogram showing THP-1 cells stained with M02690 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPT1 Antibody (M02690, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-med15-picoband-trade-antibody-monoclonal-m03568-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03568-med15-primary-antibodies-wb-testing-1.jpgAnti-MED15 Antibody Picoband® (monoclonal, 6F4) Western blot analysis of MED15 using anti-MED15 antibody (M03568).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates,<br>
Lane 4: human HL-60 whole cell lysates,<br>
Lane 5: human HepG2 whole cell lysates,<br>
Lane 6: human Hela whole cell lysates,<br>
Lane 7: human PC-3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MED15 antigen affinity purified monoclonal antibody (Catalog # M03568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MED15 at approximately 86KD. The expected band size for MED15 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03568-med15-primary-antibodies-if-testing-2.jpgAnti-MED15 Antibody Picoband® (monoclonal, 6F4) IF analysis of MED15 using anti-MED15 antibody (M03568). <br>
MED15 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MED15 Antibody (M03568) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03568-med15-primary-antibodies-fcm-testing-3.pngAnti-MED15 Antibody Picoband® (monoclonal, 6F4) Flow Cytometry analysis of U20S cells using anti- MED15 antibody (M03568). <br>Overlay histogram showing U20S cells stained with M03568 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MED15 Antibody (M03568, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/picokine-elisa-kits/human-clec1b-picokine-trade-elisa-kit-ek1921-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1921.pngHuman CLEC1B ELISA Kit PicoKine®Human CLEC1B PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-fcrl1-picokine-elisa-kit-ek1930-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1930.pngHuman FCRL1 ELISA Kit PicoKine®Human FCRL1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-tenascin-r-picokine-trade-elisa-kit-ek1931-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1931.pngHuman Tenascin-R ELISA Kit PicoKine®Human Tenascin-R PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-clec7a-picokine-trade-elisa-kit-ek1923-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1923.pngMouse CLEC7A ELISA Kit PicoKine®Mouse CLEC7A PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-osteoadherin-picokine-trade-elisa-kit-ek2017-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2017.pngHuman Osteoadherin/OSAD/OMD ELISA Kit PicoKine®Human Osteoadherin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-brevican-picokine-trade-elisa-kit-ek2019-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2019.pngHuman Brevican ELISA Kit PicoKine®Human Brevican PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-matrilin-2-picokine-trade-elisa-kit-ek2020-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2020.pngHuman Matrilin-2 ELISA Kit PicoKine®Human Matrilin-2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-matrilin-3-picokine-trade-elisa-kit-ek2022-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2022.jpgHuman Matrilin-3 ELISA Kit PicoKine®Human Matrilin-3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-smpd1-picokine-trade-elisa-kit-ek2025-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2025.pngHuman SMPD1 ELISA Kit PicoKine®Human SMPD1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-apoh-picokine-trade-elisa-kit-ek2027-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2027.jpgMouse APOH ELISA Kit PicoKine®Mouse APOH PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-osteoadherin-picokine-trade-elisa-kit-ek2018-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2018.pngMouse Osteoadherin/OSAD/OMD ELISA Kit PicoKine®Mouse Osteoadherin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-erap1-picokine-trade-elisa-kit-ek2028-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2028.jpgMouse ERAP1 ELISA Kit PicoKine®Mouse ERAP1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-matrilin-3-picokine-trade-elisa-kit-ek2023-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2023.pngMouse Matrilin-3 ELISA Kit PicoKine®Mouse Matrilin-3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-matrilin-3-picokine-trade-elisa-kit-ek2024-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2024.pngRat Matrilin-3 ELISA Kit PicoKine®Rat Matrilin-3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-matrilin-2-picokine-trade-elisa-kit-ek2021-boster.html2026-03-24T05:21:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2021.jpgMouse Matrilin-2 ELISA Kit PicoKine®Mouse Matrilin-2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-matrilin-4-picokine-trade-elisa-kit-ek2030-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2030.pngMouse Matrilin-4 ELISA Kit PicoKine®Mouse Matrilin-4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-matrilin-4-picokine-trade-elisa-kit-ek2031-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2031.pngRat Matrilin-4 ELISA Kit PicoKine®Rat Matrilin-4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-chit1-picokine-trade-elisa-kit-ek2032-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2032.jpgHuman Chitotriosidase/CHIT1 ELISA Kit PicoKine®Human CHIT1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-myeloblastin-picokine-trade-elisa-kit-ek2034-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2034.pngHuman Myeloblastin ELISA Kit PicoKine®Human Myeloblastin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-angptl7-picokine-trade-elisa-kit-ek2035-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2035.jpgHuman ANGPTL7 ELISA Kit PicoKine®Human ANGPTL7 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-tcn2-picokine-trade-elisa-kit-ek2038-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2038.pngHuman TCN2/Transcobalamin-2 ELISA Kit PicoKine®Human TCN2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-angptl2-picokine-trade-elisa-kit-ek2039-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2039.jpgHuman ANGPTL2 ELISA Kit PicoKine®Human ANGPTL2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-tpp1-picokine-trade-elisa-kit-ek2042-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2042.pngHuman TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine®Human TPP1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-chit1-picokine-trade-elisa-kit-ek2033-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2033.pngMouse CHIT1 ELISA Kit PicoKine®Mouse CHIT1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-afp-picokine-trade-elisa-kit-ek1661-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1661.jpgMouse AFP ELISA Kit PicoKine®Mouse AFP PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-ctrc-picokine-trade-elisa-kit-ek2001-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2001.pngHuman CTRC/Chymotrypsin-C ELISA Kit PicoKine®Human CTRC PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-ltbr-picokine-trade-elisa-kit-ek2002-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2002.pngHuman LTBR ELISA Kit PicoKine®Human LTBR PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-ltbr-picokine-trade-elisa-kit-ek2003-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2003.pngMouse LTBR ELISA Kit PicoKine®Mouse LTBR PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf21-dr6-picokine-trade-elisa-kit-ek2004-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2004.pngHuman TNFRSF21/DR6 ELISA Kit PicoKine®Human TNFRSF21/DR6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf22-picokine-trade-elisa-kit-ek2007-boster.html2026-03-24T05:21:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2007.pngMouse DcTRAILR2/TNFRSF22 ELISA Kit PicoKine®Mouse TNFRSF22 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf23-picokine-trade-elisa-kit-ek2008-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2008.pngMouse DcTRAILR1/TNFRSF23 ELISA Kit PicoKine®Mouse TNFRSF23 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf26-picokine-trade-elisa-kit-ek2009-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2009.pngMouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®Mouse TNFRSF26 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-sema4g-picokine-trade-elisa-kit-ek2006-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2006.pngHuman SEMA4G ELISA Kit PicoKine®Human SEMA4G PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-chi3l3-picokine-trade-elisa-kit-ek2010-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2010.pngMouse YM1/Chitinase 3-like 3/CHI3L3 ELISA Kit PicoKine®Mouse CHI3L3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-ly9-slamf3-cd229-picokine-trade-elisa-kit-ek2011-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2011.jpgMouse Ly9/SLAMF3/CD229 ELISA Kit PicoKine®Mouse Ly9/SLAMF3/CD229 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-slamf7-picokine-trade-elisa-kit-ek2012-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2012.pngMouse SLAMF7 ELISA Kit PicoKine®Mouse SLAMF7 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd39-entpd1-picokine-trade-elisa-kit-ek2013-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2013.pngMouse CD39/ENTPD1 ELISA Kit PicoKine®Mouse CD39/ENTPD1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-entpd2-picokine-trade-elisa-kit-ek2014-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2014.pngMouse ENTPD2 ELISA Kit PicoKine®Mouse ENTPD2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-midkine-picokine-trade-elisa-kit-ek2015-boster.html2026-04-01T05:01:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2015.jpgMouse Midkine ELISA Kit PicoKine®Mouse Midkine PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-midkine-picokine-trade-elisa-kit-ek2016-boster.html2026-03-24T05:21:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2016.pngRat Midkine ELISA Kit PicoKine®Rat Midkine PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/products/primary-antibodies/anti-araf-picoband-trade-antibody-a02061-2-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02061-2-araf-primary-antibodies-wb-testing-1.jpgAnti-ARAF Antibody Picoband® Western blot analysis of ARAF using anti-ARAF antibody (A02061-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: rat testis tissue lysates,<br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: mouse testis tissue lysates, <br>
Lane 6: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARAF antigen affinity purified polyclonal antibody (Catalog # A02061-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARAF at approximately 68 kDa. The expected band size for ARAF is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02061-2-araf-primary-antibodies-if-testing-2.jpgAnti-ARAF Antibody Picoband® IF analysis of ARAF using anti-ARAF antibody (A02061-2). <br>
ARAF was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARAF Antibody (A02061-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02061-2-araf-primary-antibodies-if-testing-3.jpgAnti-ARAF Antibody Picoband® IF analysis of ARAF using anti-ARAF antibody (A02061-2). <br>
ARAF was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARAF Antibody (A02061-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02061-2-araf-primary-antibodies-fcm-testing-4.jpgAnti-ARAF Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ARAF antibody (A02061-2). <br>Overlay histogram showing SiHa cells stained with A02061-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARAF Antibody (A02061-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-arid2-picoband-trade-antibody-a05064-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-fonc-13-1285508-g003.jpgAnti-ARID2 Antibody Picoband®Prognostic associated somatic mutated genes in FPHYP CRC cohort. (A) Univariable analyses of PFS concerning somatic gene mutations in FPHYP CRC tumors. (B) Kaplan-Meier curves for PFS between three genes( BRAF , ARID2 , and KMT2C ) combined MT and WT groups. (C–E) Kaplan-Meier curves for PFS based on BRAF (C) , ARID2 (D) , and KMT2C (E) mutation status. (F, G) Kaplan-Meier plots of PFS for CRC patients undergoing exclusive first-line chemotherapy (F) and chemotherapy combined with bevacizumab (G) , stratified by BRAF mutation status. PFS, progression-free survival; MT, mutation type; WT, wiled type.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1285508/full'>38023196</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-fonc-13-1285508-g004.jpgAnti-ARID2 Antibody Picoband®Prognostic associated somatic mutated genes in FPHYP CRC cohort. (A) Univariable analyses of OS concerning somatic gene mutations in FPHYP CRC tumors. (B) Kaplan-Meier curves for OS between three genes( BRAF , ARID2 , and KMT2C ) combined MT and WT groups. (C–E) Kaplan-Meier curves for PFS based on BRAF (C) , ARID2 (D) , and KMT2C (E) mutation status. OS, overall survival; MT, mutation type; WT, wiled type.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1285508/full'>38023196</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-fonc-13-1285508-g007.jpgAnti-ARID2 Antibody Picoband®Construction of a four-gene mutation signature prediction disease progression and prognosis in FPHYP cohort. (A, B) Univariate and multivariate analyses were performed to assess the impact of clinicopathological features, individual somatic gene mutations, and the four-gene mutation signature on PFS (A) and OS (B) in CRC. (C) The Kaplan-Meier survival analysis for PFS in CRC patients between the four-gene combined MT and WT groups based on BRAF , ARID2 , KMT2C , and GNAQ mutation status. (D) The Kaplan-Meier survival analysis for OS in CRC cases between the four-gene combined MT and WT groups based on BRAF , ARID2 , KMT2C , and GNAQ mutation status. (E, F) ROC curves for PFS (E) and OS (F) that dependent on time were generated to evaluate the prognostic model’s performance, which is based on the gene mutation status within the FPHYP cohort. PFS, progression-free survival; OS, overall survival; MT, mutation type; WT, wiled type; ROC, receiver operating characteristic.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1285508/full'>38023196</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-fonc-13-1285508-g010.jpgAnti-ARID2 Antibody Picoband®Immunohistochemical analysis of BRAF and ARID2 in CRC. (A, B) The BRAF expression original field was acquired from tissue sections (magnification, 200x) of the BRAF -MT (A) and BRAF -WT (B) groups. (C) Comparison of the IOD/Area value between BRAF -MT and BRAF -WT groups. (D, E) The BRAF expression original field was acquired from tissue sections (magnification, 200x) of stage I (D) and stage IV (E) groups. (F) Comparison of the IOD/Area value between stage I and IV groups. (G, H) The ARID2 expression original field was acquired from tissue sections (magnification, 200x) of the ARID2 -MT (G) and ARID2 -WT (H) groups. (I) Comparison of the IOD/Area value between ARID2 -MT and ARID2 -WT groups. (J, K) The ARID2 expression original field was acquired from tissue sections (magnification, 200x) of stage I (J) and stage IV (K) groups. (L) Comparison of the IOD/Area value between stage I and IV groups. MT, mutation type; WT, wiled type; IOD, cumulative optical density.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2023.1285508/full'>38023196</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-arid2-primary-antibodies-wb-testing-1.jpgAnti-ARID2 Antibody Picoband® Western blot analysis of ARID2 using anti-ARID2 antibody (A05064). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human HEL whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (A05064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05064-arid2-primary-antibodies-ip-testing-2.jpgAnti-ARID2 Antibody Picoband® Immunoprecipitating ARID2 in MCF-7 whole cell lysate.<br>
Western blot analysis of ARID2 using anti-ARID2 antibody (A05064).<br>
Lane 1: MCF-7 whole cell lysates (30ug),<br>
Lane 2: Rabbit control IgG instead of anti-ARID2 antibody in MCF-7 whole cell lysate,<br>
Lane 3: anti-ARID2 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (A05064) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ARID2 at approximately 245 kDa. The expected band size for ARID2 is at 197 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-arp2-3-subunit-1b-arpc1b-picoband-trade-antibody-a07192-2-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07192-2-arpc1b-primary-antibodies-wb-testing-1.jpgAnti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® Western blot analysis of ARPC1B using anti-ARPC1B antibody (A07192-2). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates,<br>
Lane 2: human U937 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: mouse spleen tissue lysates,<br>
Lane 7: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPC1B antigen affinity purified polyclonal antibody (A07192-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARPC1B at approximately 37 kDa. The expected band size for ARPC1B is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07192-2-arpc1b-primary-antibodies-ihc-testing-2.jpgAnti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® IHC analysis of ARPC1B using anti-ARPC1B antibody (A07192-2). <br>ARPC1B was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARPC1B Antibody (A07192-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07192-2-arpc1b-primary-antibodies-fcm-testing-3.jpgAnti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ARPC1B antibody (A07192-2). <br>
Overlay histogram showing U937 cells stained with A07192-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARPC1B Antibody (A07192-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07192-2-arpc1b-primary-antibodies-ip-testing-4.jpgAnti-ARP2/3 subunit 1B/ARPC1B Antibody Picoband® Immunoprecipitating (IP) ARPC1B in Hela whole cell lysate.<br>
Western blot analysis of ARPC1B using anti-ARPC1B antibody (A07192-2); <br>
Lane 1: Hela whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-ARPC1B antibody in Hela whole cell lysate;<br>
Lane 3: anti-ARPC1B antibody (2μg) + Hela whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARPC1B antigen affinity purified polyclonal antibody (A07192-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ARPC1B at approximately 37 kDa. The expected band size for ARPC1B is at 41 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-atp5f1-atp5mc1-picoband-trade-antibody-a09735-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-if-testing-1.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IF analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735). <br>
ATP5G1,2,3 was detected in paraffin-embedded section of human intestinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-10.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1-2-3-primary-antibodies-fc-testing-2.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband®2. Flow Cytometry analysis of HL-60 cells using anti-ATP5G1,2,3 antibody (A09735). <br>Overlay histogram showing HL-60 cells stained with A09735 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5G1,2,3 Antibody (A09735,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1-2-3-primary-antibodies-fc-testing-3.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband®3. Flow Cytometry analysis of PC-3 cells using anti-ATP5G1,2,3 antibody (A09735). <br>Overlay histogram showing HL-60 cells stained with A09735 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5G1,2,3 Antibody (A09735,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-4.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-5.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-6.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-7.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-8.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-ihc-testing-9.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
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ATP5G1,2,3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-wb-testing-11.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® Western blot analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEPG2 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human HL-60 whole cell lysates, <br>
Lane 5: human HEK293 whole cell lysates, <br>
Lane 6: human U937 whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates, <br>
Lane 8 :rat kidney tissue lysates, <br>
Lane 9: rat stomach tissue lysates, <br>
Lane 10: rat liver tissue lysates, <br>
Lane 11: mouse kidney tissue lysates, <br>
Lane 12: mouse stomach tissue lysates, <br>
Lane 13: mouse liver tissue lysates, <br>
Lane 14: mouse lung tissue lysates, <br>
Lane 15: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5G1,2,3 antigen affinity purified polyclonal antibody (Catalog # A09735) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5G1,2,3 at approximately 10-14KD. The expected band size for ATP5G1,2,3 is at 10-14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09735-atp5g1_2_3-primary-antibodies-if-testing-12.jpgAnti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband® IF analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735). <br>
ATP5G1,2,3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-bcl9l-picoband-trade-antibody-a05905-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05905-bcl9l-primary-antibodies-wb-testing-1.jpgAnti-BCL9L Antibody Picoband®Western blot analysis of BCL9L using anti-BCL9L antibody (A05905). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL9L antigen affinity purified polyclonal antibody (A05905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL9L at approximately 150-220 kDa. The expected band size for BCL9L is at 157 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05905-bcl9l-primary-antibodies-wb-testing-2.jpgAnti-BCL9L Antibody Picoband®Western blot analysis of BCL9L using anti-BCL9L antibody (A05905). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates,<br>
Lane 2: rat PC-12 whole cell lysates,<br>
Lane 3: mouse testis tissue lysates,<br>
Lane 4: mouse thymus tissue lysates,<br>
Lane 5: mouse HEPA1/6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL9L antigen affinity purified polyclonal antibody (A05905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL9L at approximately 150-220 kDa. The expected band size for BCL9L is at 157 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05905-bcl9l-primary-antibodies-ihc-testing-1.jpgAnti-BCL9L Antibody Picoband®IHC analysis of BCL9L using anti-BCL9L antibody (A05905). <br>BCL9L was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL9L Antibody (A05905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05905-bcl9l-primary-antibodies-fcm-testing-1.jpgAnti-BCL9L Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-BCL9L antibody (A05905). <br>
Overlay histogram showing MCF-7 cells stained with A05905 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL9L Antibody (A05905, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ccr2-picoband-trade-antibody-a00158-4-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-CCR2 Antibody Picoband®Boster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00158-4-CCR2-primary-antibodies-WB-testing-1.jpgAnti-CCR2 Antibody Picoband® Western blot analysis of CCR2 using anti-CCR2 antibody (A00158-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: rat spleen issue lysates, <br>
Lane 5: rat kidney issue lysates, <br>
Lane 6: mouse spleen issue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # A00158-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 45KD. The expected band size for CCR2 is at 42KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cd8-alpha-cd8a-picoband-trade-antibody-a02236-2-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02236-2-CD8A-primary-antibodies-IHC-testing-1.jpgAnti-CD8 alpha/Cd8a Antibody Picoband® IHC analysis of CD8A using anti-CD8A antibody (A02236-2).
CD8A was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8A Antibody (A02236-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02236-2-CD8A-primary-antibodies-IHC-testing-2.jpgAnti-CD8 alpha/Cd8a Antibody Picoband® IHC analysis of CD8A using anti-CD8A antibody (A02236-2).
<br>
CD8A was detected in paraffin-embedded section of rat thymus tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD8A Antibody (A02236-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02236-2-CD8A-primary-antibodies-WB-testing-3.jpgAnti-CD8 alpha/Cd8a Antibody Picoband® Western blot analysis of CD8A using anti-CD8A antibody (A02236-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat thymus issue lysates, <br>
Lane 2: rat thymus issue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8A antigen affinity purified polyclonal antibody (Catalog # A02236-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8A at approximately 26-35KD. The expected band size for CD8A is at 26KD.
https://www.bosterbio.com/products/primary-antibodies/anti-pan-cadherin-cdh4-picoband-trade-antibody-a07632-2-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07632-2-cdh4-primary-antibodies-wb-testing-1.jpgAnti-R-Cadherin-4 CDH4 Antibody Picoband® Western blot analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody (A07632-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates, <br>
Lane 2: human U87 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human CACO-2 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human HELA whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-R Cadherin/CDH4 antigen affinity purified polyclonal antibody (Catalog # A07632-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for R Cadherin/CDH4 at approximately 125KD. The expected band size for R Cadherin/CDH4 is at 125KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07632-2-cdh4-primary-antibodies-ihc-testing-2.jpgAnti-R-Cadherin-4 CDH4 Antibody Picoband® IHC analysis of R Cadherin/CDH4 using anti-R Cadherin/CDH4 antibody (A07632-2). <br>
R Cadherin/CDH4 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-R Cadherin/CDH4 Antibody (A07632-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07632-2-cdh4-primary-antibodies-fcm-testing-3.pngAnti-R-Cadherin-4 CDH4 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-R Cadherin/CDH4 antibody (A07632-2). <br>Overlay histogram showing U20S cells stained with A07632-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-R Cadherin/CDH4 Antibody (A07632-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-2b6-cyp2b6-picoband-trade-antibody-a00861-2-boster.html2026-03-24T05:23:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00861-2-CYP2B6-primary-antibodies-WB-testing-1.jpgAnti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® Western blot analysis of CYP2B6 using anti-CYP2B6 antibody (A00861-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Caco-2 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human HL-60 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates, <br>
Lane 6: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2B6 antigen affinity purified polyclonal antibody (Catalog # A00861-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2B6 at approximately 56KD. The expected band size for CYP2B6 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00861-2-cyp2b6-primary-antibodies-if-testing-2.jpgAnti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® IF analysis of Cytochrome P450 2B6/CYP2B6 using anti-Cytochrome P450 2B6/CYP2B6antibody (A00861-2). <br>
Cytochrome P450 2B6/CYP2B6was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Cytochrome P450 2B6/CYP2B6 Antibody (A00861-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00861-2-cyp2b6-primary-antibodies-fcm-testing-3.pngAnti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Cytochrome P450 2B6/CYP2B6 antibody (A00861-2). <br>Overlay histogram showing THP-1 cells stained with A00861-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 2B6/CYP2B6 Antibody (A00861-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ehd1-picoband-trade-antibody-a02168-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02168-1-ehd1-primary-antibodies-fc-testing-1.pngAnti-EHD1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-EHD1 antibody (A02168-1). <br> Overlay histogram showing U20S cells stained with A02168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD1 Antibody (A02168-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02168-1-ehd1-primary-antibodies-fc-testing-2.pngAnti-EHD1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-EHD1 antibody (A02168-1). <br> Overlay histogram showing U87 cells stained with A02168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD1 Antibody (A02168-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02168-1-ehd1-primary-antibodies-if-testing-3.jpgAnti-EHD1 Antibody Picoband® IF analysis of EHD1 using anti-EHD1 antibody (A02168-1). <br> EHD1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02168-1-EHD1-primary-antibodies-IHC-testing-1.jpgAnti-EHD1 Antibody Picoband® IHC analysis of EHD1 using anti-EHD1 antibody (A02168-1).<br>EHD1 was detected in paraffin-embedded section of human testis cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02168-1-EHD1-primary-antibodies-IHC-testing-2.jpgAnti-EHD1 Antibody Picoband® IHC analysis of EHD1 using anti-EHD1 antibody (A02168-1).<br>EHD1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02168-1-EHD1-primary-antibodies-IHC-testing-3.jpgAnti-EHD1 Antibody Picoband® IHC analysis of EHD1 using anti-EHD1 antibody (A02168-1).<br>EHD1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02168-1-EHD1-primary-antibodies-IHC-testing-4.jpgAnti-EHD1 Antibody Picoband® IHC analysis of EHD1 using anti-EHD1 antibody (A02168-1).<br>EHD1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02168-1-EHD1-primary-antibodies-IHC-testing-5.jpgAnti-EHD1 Antibody Picoband® IHC analysis of EHD1 using anti-EHD1 antibody (A02168-1).<br>EHD1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EHD1 Antibody (A02168-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02168-1-ehd1-primary-antibodies-wb-testing-9_1.jpgAnti-EHD1 Antibody Picoband® Western blot analysis of EHD1 using anti-EHD1 antibody (A02168-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human U87 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: rat testis tissue lysates, <br>
Lane 5: rat testis tissue lysates, <br>
Lane 6: rat kidney tissue lysates, <br>
Lane 7: rat ovary tissue lysates, <br>
Lane 8: mouse testis tissue lysates, <br>
Lane 9: mouse testis tissue lysates, <br>
Lane 10: mouse kidney tissue lysates, <br>
Lane 11: mouse ovary tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD1 antigen affinity purified polyclonal antibody (Catalog # A02168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD1 at approximately 61 kDa. The expected band size for EHD1 is at 61 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-gpd1l-picoband-trade-antibody-a07912-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07912-1-gpd1l-primary-antibodies-wb-testing-1.jpgAnti-GPD1L Antibody Picoband®Western blot analysis of GPD1L using anti-GPD1L antibody (A07912-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: rat heart tissue lysates,<br>
Lane 4: rat skeletal muscle tissue lysates,<br>
Lane 5: mouse heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPD1L antigen affinity purified polyclonal antibody (Catalog # A07912-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPD1L at approximately 35-40 kDa. The expected band size for GPD1L is at 38 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-herc5-picoband-trade-antibody-a05285-2-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05285-2-herc5-primary-antibodies-wb-testing-1.jpgAnti-HERC5 Antibody Picoband® Western blot analysis of HERC5 using anti-HERC5 antibody (A05285-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HERC5 antigen affinity purified polyclonal antibody (Catalog # A05285-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HERC5 at approximately 117 kDa. The expected band size for HERC5 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05285-2-herc5-primary-antibodies-if-testing-2_1.jpgAnti-HERC5 Antibody Picoband® IF analysis of HERC5 using anti-HERC5 antibody (A05285-2). <br>
HERC5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HERC5 Antibody (A05285-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-kif15-picoband-trade-antibody-a05983-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-wb-testing-1.jpgAnti-KIF15 Antibody Picoband® Western blot analysis of KIF15 using anti-KIF15 antibody (A05983-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates, <br>
Lane 2: human U20S whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF15 antigen affinity purified polyclonal antibody (Catalog # A05983-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF15 at approximately 150-160KD. The expected band size for KIF15 is at 150-160KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-ihc-testing-2.jpgAnti-KIF15 Antibody Picoband® IHC analysis of KIF15 using anti-KIF15 antibody (A05983-1). <br>
KIF15 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KIF15 Antibody (A05983-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-ihc-testing-3.jpgAnti-KIF15 Antibody Picoband® IHC analysis of KIF15 using anti-KIF15 antibody (A05983-1). <br>
KIF15 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KIF15 Antibody (A05983-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-if-testing-4.jpgAnti-KIF15 Antibody Picoband® IF analysis of KIF15 using anti-KIF15 antibody (A05983-1). <br>
KIF15 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-KIF15 Antibody (A05983-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-fcm-testing-5.pngAnti-KIF15 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-KIF15 antibody (A05983-1). <br>Overlay histogram showing SiHa cells stained with A05983-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIF15 Antibody (A05983-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05983-1-kif15-primary-antibodies-fcm-testing-6.pngAnti-KIF15 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-KIF15 antibody (A05983-1). <br>Overlay histogram showing U20S cells stained with A05983-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIF15 Antibody (A05983-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mpp1-kif20b-picoband-trade-antibody-a08340-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-MPP1/KIF20B Antibody Picoband®Boster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08340-KIF20B-primary-antibodies-WB-testing-1.jpgAnti-MPP1/KIF20B Antibody Picoband® Western blot analysis of KIF20B using anti-KIF20B antibody (A08340). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF20B antigen affinity purified polyclonal antibody (Catalog # A08340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF20B at approximately 211KD. The expected band size for KIF20B is at 211KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mek4-map2k4-picoband-trade-antibody-a01725-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01725-1-MAP2K4-primary-antibodies-WB-testing-1.jpgAnti-MEK4/MAP2K4 Antibody Picoband® Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: human MCF-7 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human 22RV1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01725-1-MAP2K4-primary-antibodies-WB-testing-2.jpgAnti-MEK4/MAP2K4 Antibody Picoband® Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: rat skeletal muscle tissue lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse kidney tissue lysates, <br>
Lane 6: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mtus1-picoband-trade-antibody-a04047-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04047-1-MTUS1-primary-antibodies-IF-testing-1.jpgAnti-MTUS1 Antibody Picoband® IF analysis of MTUS1 using anti-MTUS1 antibody (A04047-1). <br> MTUS1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MTUS1 Antibody (A04047-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04047-1-MTUS1-primary-antibodies-WB-testing-2.jpgAnti-MTUS1 Antibody Picoband® Western blot analysis of MTUS1 using anti-MTUS1 antibody (A04047-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTUS1 antigen affinity purified polyclonal antibody (Catalog # A04047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTUS1 at approximately 170KD. The expected band size for MTUS1 is at 141KD.
https://www.bosterbio.com/products/primary-antibodies/anti-muc13-picoband-trade-antibody-a02674-2-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02674-2-muc13-primary-antibodies-wb-testing-1.jpgAnti-MUC13 Antibody Picoband®Western blot analysis of MUC13 using anti-MUC13 antibody (A02674-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Caco-2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: rat stomach tissue lysates,<br>
Lane 5: mouse stomach tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC13 antigen affinity purified polyclonal antibody (A02674-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MUC13 at approximately 75, 110-120 kDa. The expected band size for MUC13 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02674-2-muc13-primary-antibodies-ihc-testing-1.jpgAnti-MUC13 Antibody Picoband®IHC analysis of MUC13 using anti-MUC13 antibody (A02674-2). <br>MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC13 Antibody (A02674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02674-2-muc13-primary-antibodies-ihc-testing-2.jpgAnti-MUC13 Antibody Picoband®IHC analysis of MUC13 using anti-MUC13 antibody (A02674-2). <br>MUC13 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC13 Antibody (A02674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02674-2-muc13-primary-antibodies-if-testing-1.jpgAnti-MUC13 Antibody Picoband®IF analysis of AMUC13 using anti-MUC13 antibody (A02674-2). <br>
MUC13 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MUC13 Antibody (A02674-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-nalp2-nlrp2-picoband-trade-antibody-a04819-2-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04819-2-NLRP2-primary-antibodies-WB-testing-1.jpgAnti-NALP2/NLRP2 Antibody Picoband® Western blot analysis of NLRP2 using anti-NLRP2 antibody (A04819-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP2 antigen affinity purified polyclonal antibody (Catalog # A04819-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP2 at approximately 121KD. The expected band size for NLRP2 is at 121KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04819-2-NLRP2-primary-antibodies-WB-testing-2.jpgAnti-NALP2/NLRP2 Antibody Picoband® Western blot analysis of NLRP2 using anti-NLRP2 antibody (A04819-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP2 antigen affinity purified polyclonal antibody (Catalog # A04819-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP2 at approximately 121KD. The expected band size for NLRP2 is at 121KD.
https://www.bosterbio.com/products/primary-antibodies/anti-onecut2-picoband-trade-antibody-a09652-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09652-1-ONECUT2-primary-antibodies-WB-testing-1.jpgAnti-ONECUT2 Antibody Picoband® Western blot analysis of ONECUT2 using anti-ONECUT2 antibody (A09652-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT2 antigen affinity purified polyclonal antibody (Catalog # A09652-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT2 at approximately 54KD. The expected band size for ONECUT2 is at 54KD.
https://www.bosterbio.com/products/primary-antibodies/anti-plasminogen-plg-picoband-trade-antibody-a00702-1-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00702-1-plg-primary-antibodies-wb-testing-1.jpgAnti-Plasminogen/PLG Antibody Picoband® Western blot analysis of PLG using anti-PLG antibody (A00702-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
Lane 4: mouse spleen tissue lysates. <br>
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLG antigen affinity purified polyclonal antibody (Catalog # A00702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLG at approximately 90-100 kDa. The expected band size for PLG is at 90-100 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-ppa2-picoband-trade-antibody-a06587-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-wb-testing-1.jpgAnti-PPA2 Antibody Picoband® Western blot analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: mouse heart tissue lysates,<br>
Lane 6: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPA2 antigen affinity purified polyclonal antibody (Catalog # A06587) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPA2 at approximately 35 kDa. The expected band size for PPA2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-2.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-3.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-4.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-5.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-6.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-ihc-testing-7.jpgAnti-PPA2 Antibody Picoband® IHC analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06587-ppa2-primary-antibodies-if-testing-8.jpgAnti-PPA2 Antibody Picoband® IF analysis of PPA2 using anti-PPA2 antibody (A06587). <br>
PPA2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPA2 Antibody (A06587) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-pyroxd1-picoband-trade-antibody-a17214-3-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-IHC-testing-1.jpgAnti-PYROXD1 Antibody Picoband® IHC analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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PYROXD1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PYROXD1 Antibody (A17214-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-IHC-testing-2.jpgAnti-PYROXD1 Antibody Picoband® IHC analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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PYROXD1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PYROXD1 Antibody (A17214-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-IHC-testing-3.jpgAnti-PYROXD1 Antibody Picoband® IHC analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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PYROXD1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PYROXD1 Antibody (A17214-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-IHC-testing-4.jpgAnti-PYROXD1 Antibody Picoband® IHC analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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PYROXD1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PYROXD1 Antibody (A17214-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-IHC-testing-5.jpgAnti-PYROXD1 Antibody Picoband® IHC analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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PYROXD1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PYROXD1 Antibody (A17214-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A17214-3-PYROXD1-primary-antibodies-WB-testing-6.jpgAnti-PYROXD1 Antibody Picoband® Western blot analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-3).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human placenta tissue lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human HL-60 whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: rat kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYROXD1 antigen affinity purified polyclonal antibody (Catalog # A17214-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYROXD1 at approximately 50-56KD. The expected band size for PYROXD1 is at 50KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rab3gap2-picoband-trade-antibody-a07244-3-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-IHC-testing-1.jpgAnti-RAB3GAP2 Antibody Picoband® IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3).
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RAB3GAP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07244-3-rab3gap2-primary-antibodies-ihc-testing-6.jpgAnti-RAB3GAP2 Antibody Picoband®IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3). <br>RAB3GAP2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-IHC-testing-2.jpgAnti-RAB3GAP2 Antibody Picoband® IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3).
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RAB3GAP2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-IHC-testing-3.jpgAnti-RAB3GAP2 Antibody Picoband® IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3).
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RAB3GAP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-IHC-testing-4.jpgAnti-RAB3GAP2 Antibody Picoband® IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3).
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RAB3GAP2 was detected in paraffin-embedded section of rat intestine tissues.<br> Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-IHC-testing-5.jpgAnti-RAB3GAP2 Antibody Picoband® IHC analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3).
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RAB3GAP2 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RAB3GAP2 Antibody (A07244-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07244-3-RAB3GAP2-primary-antibodies-FC-testing-6.jpgAnti-RAB3GAP2 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-RAB3GAP2 antibody (A07244-3).
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Overlay histogram showing CACO-2 cells stained with A07244-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB3GAP2 Antibody (A07244-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07244-3-rab3gap2-primary-antibodies-wb-testing-7.jpgAnti-RAB3GAP2 Antibody Picoband® Western blot analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HL-60 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: human Raji whole cell lysates, <br> Lane 5: human Hela whole cell lysates, <br> Lane 6: human HepG2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3GAP2 antigen affinity purified polyclonal antibody (Catalog # A07244-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB3GAP2 at approximately 156KD. The expected band size for RAB3GAP2 is at 156KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07244-3-rab3gap2-primary-antibodies-wb-testing-8.jpgAnti-RAB3GAP2 Antibody Picoband® Western blot analysis of RAB3GAP2 using anti-RAB3GAP2 antibody (A07244-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysates, <br> Lane 2: mouse kidney tissue lysates, <br> Lane 3: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3GAP2 antigen affinity purified polyclonal antibody (Catalog # A07244-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB3GAP2 at approximately 156KD. The expected band size for RAB3GAP2 is at 156KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rbms3-picoband-trade-antibody-a12416-3-boster.html2026-03-24T05:23:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12416-3-RBMS3-primary-antibodies-IF-testing-1.jpgAnti-RBMS3 Antibody Picoband® IF analysis of RBMS3 using anti-RBMS3 antibody (A12416-3). <br> RBMS3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RBMS3 Antibody (A12416-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12416-3-RBMS3-primary-antibodies-IHC-testing-2.jpgAnti-RBMS3 Antibody Picoband® IHC analysis of RBMS3 using anti-RBMS3 antibody (A12416-3).
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RBMS3 was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBMS3 Antibody (A12416-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12416-3-RBMS3-primary-antibodies-IHC-testing-3.jpgAnti-RBMS3 Antibody Picoband® IHC analysis of RBMS3 using anti-RBMS3 antibody (A12416-3).
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RBMS3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBMS3 Antibody (A12416-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A12416-3-RBMS3-primary-antibodies-IHC-testing-4.jpgAnti-RBMS3 Antibody Picoband® IHC analysis of RBMS3 using anti-RBMS3 antibody (A12416-3).
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RBMS3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBMS3 Antibody (A12416-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12416-3-rbms3-primary-antibodies-wb-testing-5.jpgAnti-RBMS3 Antibody Picoband® Western blot analysis of RBMS3 using anti-RBMS3 antibody (A12416-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U2OS whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human U-87MG whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human Hela whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS3 antigen affinity purified polyclonal antibody (Catalog # A12416-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMS3 at approximately 55KD. The expected band size for RBMS3 is at 48-55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12416-3-rbms3-primary-antibodies-wb-testing-6.jpgAnti-RBMS3 Antibody Picoband® Western blot analysis of RBMS3 using anti-RBMS3 antibody (A12416-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: mouse testicular tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS3 antigen affinity purified polyclonal antibody (Catalog # A12416-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMS3 at approximately 55KD. The expected band size for RBMS3 is at 48-55KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf123-picoband-trade-antibody-a09642-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-IHC-testing-1.jpgAnti-RNF123 Antibody Picoband® IHC analysis of RNF123 using anti-RNF123 antibody (A09642-1).
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RNF123 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF123 Antibody (A09642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-IHC-testing-2.jpgAnti-RNF123 Antibody Picoband® IHC analysis of RNF123 using anti-RNF123 antibody (A09642-1).
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RNF123 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF123 Antibody (A09642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-IHC-testing-3.jpgAnti-RNF123 Antibody Picoband® IHC analysis of RNF123 using anti-RNF123 antibody (A09642-1).
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RNF123 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF123 Antibody (A09642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-IHC-testing-4.jpgAnti-RNF123 Antibody Picoband® IHC analysis of RNF123 using anti-RNF123 antibody (A09642-1).
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RNF123 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF123 Antibody (A09642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-IHC-testing-5.jpgAnti-RNF123 Antibody Picoband® IHC analysis of RNF123 using anti-RNF123 antibody (A09642-1).
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RNF123 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF123 Antibody (A09642-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-WB-testing-6.jpgAnti-RNF123 Antibody Picoband® Western blot analysis of RNF123 using anti-RNF123 antibody (A09642-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human THP-1 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human U-87MG whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF123 antigen affinity purified polyclonal antibody (Catalog # A09642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF123 at approximately 149KD. The expected band size for RNF123 is at 149KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09642-1-RNF123-primary-antibodies-WB-testing-7.jpgAnti-RNF123 Antibody Picoband® Western blot analysis of RNF123 using anti-RNF123 antibody (A09642-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular tissue lysates, <br>
Lane 2: mouse testicular tissue lysates, <br>
Lane 3: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF123 antigen affinity purified polyclonal antibody (Catalog # A09642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF123 at approximately 149KD. The expected band size for RNF123 is at 149KD.
https://www.bosterbio.com/products/primary-antibodies/anti-syntenin-sdcbp-picoband-trade-antibody-a02475-2-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02475-2-SDCBP-primary-antibodies-WB-testing-1.jpgAnti-Syntenin/SDCBP Antibody Picoband® Western blot analysis of SDCBP using anti-SDCBP antibody (A02475-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCBP antigen affinity purified polyclonal antibody (Catalog # A02475-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCBP at approximately 32KD. The expected band size for SDCBP is at 32KD.
https://www.bosterbio.com/products/primary-antibodies/anti-semaphorin-3e-sema3e-picoband-trade-antibody-a06937-2-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06937-2-SEMA3E-primary-antibodies-WB-testing-1.jpgAnti-Semaphorin 3E/SEMA3E Antibody Picoband® Western blot analysis of SEMA3E using anti-SEMA3E antibody (A06937-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3E antigen affinity purified polyclonal antibody (Catalog # A06937-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3E at approximately 89KD. The expected band size for SEMA3E is at 89KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06937-2-SEMA3E-primary-antibodies-WB-testing-2.jpgAnti-Semaphorin 3E/SEMA3E Antibody Picoband® Western blot analysis of SEMA3E using anti-SEMA3E antibody (A06937-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3E antigen affinity purified polyclonal antibody (Catalog # A06937-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3E at approximately 89KD. The expected band size for SEMA3E is at 89KD.
https://www.bosterbio.com/products/primary-antibodies/anti-glut6-slc2a6-picoband-trade-antibody-a11862-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11862-1-SLC2A6-primary-antibodies-WB-testing-1.jpgAnti-GLUT6/SLC2A6 Antibody Picoband® Western blot analysis of SLC2A6 using anti-SLC2A6 antibody (A11862-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human PC-3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A6 antigen affinity purified polyclonal antibody (Catalog # A11862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A6 at approximately 55-60KD. The expected band size for SLC2A6 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A11862-1-SLC2A6-primary-antibodies-WB-testing-2.jpgAnti-GLUT6/SLC2A6 Antibody Picoband® Western blot analysis of SLC2A6 using anti-SLC2A6 antibody (A11862-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: rat lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A6 antigen affinity purified polyclonal antibody (Catalog # A11862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A6 at approximately 55-60KD. The expected band size for SLC2A6 is at 55KD.
https://www.bosterbio.com/products/primary-antibodies/anti-sglt2-slc5a2-picoband-trade-antibody-a03748-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-slc5a2-primary-antibodies-wb-testing-1.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates, <br> Lane 2: human THP-1 whole cell lysates, <br> Lane 3: rat kidney tissue lysates, <br> Lane 4: rat spleen tissue lysates, <br> Lane 5: rat lung tissue lysates, <br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse spleen tissue lysates, <br> Lane 8: mouse lung tissue lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-fphar-12-741087-g005.jpgAnti-SGLT2/SLC5A2 Antibody Picoband®High-salt diet alters SGLT2 and Na+/K + -ATPase expression in renal tubules of DKD mice. (A) Expression of SGLT2 gene in renal tubules ( n = 6 per group). (B) Paraffin-embedded renal sections were stained with SGLT2, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (C) Histopathological assessment of SGLT2, ATP1A1 and ATP1B1 proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; ## p < 0.01 and ### p < 0.001 vs. DM group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.741087/full'>34987387</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-fphar-12-741087-g006.jpgAnti-SGLT2/SLC5A2 Antibody Picoband®High salt alters SGLT2 and Na+/K + -ATPase expression in HG-treated HK-2. (A) Intracellular glucose concentration. (B,C) Expression of SGLT2 in HK-2 in HK-2 cultured with HG and 15 mM NaCl. (D) Expression of NKAIN4, ATP1A1, ATP1B1 and ATP1B3 genes in HK-2 after exposure to HG and different concentrations of NaCl. (E,F) Expression of ATP1A1 protein in HK-2 with HG and 15 mM NaCl cultured. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. Control group; # p < 0.05 and ## p < 0.01 vs. HG group. (G) Expression of NKAIN4 and fatty acid metabolism related genes were detected in HK-2 treated with Digoxin. * p < 0.05 and *** p < 0.001 vs. DM + DMSO group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. HG + NaCl + DMSO group. All data are mean ± SEM, n = 3 per group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.741087/full'>34987387</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-fphar-12-741087-g007.jpgAnti-SGLT2/SLC5A2 Antibody Picoband®High-salt diet attenuates gene and protein expression in diabetic mice treated with dapagliflozin. (A) The detection of ACR ( n = 6 per group). (B) Expression of α Sma, Col-1 and Slc5a2 genes in renal tubules after dapagliflozin treatment ( n = 6 per group). (C) Paraffin-embedded renal sections were stained with FN and SGLT2 antibodies (magnification, 400×, bar = 20 μm). (D) Histopathological assessment of FN and SGLT2 proteins ( n = 4 per group). Expression of genes involved in fatty acid metabolism (E) and mitochondrial biosynthesis (H) in renal tubules after dapagliflozin treatment ( n = 6 per group). (F,I) Paraffin-embedded renal sections were stained with CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1 antibodies (magnification, 400×, bar = 20 μm). (G,J) Histopathological assessment of CPT1A, ACOX1, FABP4, FASN, ATP1A1 and ATP1B1proteins ( n = 4 per group). All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DM + Saline group; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. DM + Dapa group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.741087/full'>34987387</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-fphar-12-741087-g008.jpgAnti-SGLT2/SLC5A2 Antibody Picoband®High salt attenuates gene and protein expression in HG-cultured HK-2 treated with dapagliflozin. Expression of fibrosis-related genes (A) and Na+/K + -ATPase-related genes (B) in HG-treated HK-2 after exposure to different concentrations of dapagliflozin. n = 4 per group. (C,D) Expression of SGLT2, FN and ATP1A1 proteins in HG-treated HK-2 after exposure to 5uM and 10uM dapagliflozin. n = 3 per group. (E) Expression of fatty acid metabolism-related genes. n = 3 per group. (F,G) Expression of FN, ATP1A1 and CPT1A proteins in HK-2 co-cultured with HG, 15 mM NaCl and 5 uM dapagliflozin. n = 3 per group. All data are mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. HG + DMSO group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.741087/full'>34987387</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-slc5a2-primary-antibodies-ihc-testing-2.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). <br> SLC5A2 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-slc5a2-primary-antibodies-ihc-testing-3.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). <br> SLC5A2 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03748-1-SLC5A2-primary-antibodies-IHC-testing-3.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).<br>SLC5A2 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03748-1-slc5a2-primary-antibodies-fc-testing-5.pngAnti-SGLT2/SLC5A2 Antibody Picoband®5. Flow Cytometry analysis of HepG2 cells using anti-SLC5A2 antibody (A03748-1). <br> Overlay histogram showing HepG2 cells stained with A03748-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A2 Antibody (A03748-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03748-1-SLC5A2-primary-antibodies-WB-testing-6.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates, <br>
Lane 2: human THP-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03748-1-SLC5A2-primary-antibodies-WB-testing-7.jpgAnti-SGLT2/SLC5A2 Antibody Picoband® Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat kidney tissue lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: rat lung tissue lysates, <br>
Lane 4: mouse kidney tissue lysates, <br>
Lane 5: mouse spleen tissue lysates, <br>
Lane 6: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ebp50-nherf-1-slc9a3r1-picoband-trade-antibody-a02427-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-1.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
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SLC9A3R1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-2.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
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SLC9A3R1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-3.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
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SLC9A3R1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-4.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
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SLC9A3R1 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02427-1-SLC9A3R1-primary-antibodies-IHC-testing-5.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® IHC analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1).
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SLC9A3R1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A3R1 Antibody (A02427-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02427-1-ebp50-primary-antibodies-wb-testing-6.jpgAnti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband® Western blot analysis of SLC9A3R1 using anti-SLC9A3R1 antibody (A02427-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysate, <br> Lane 2: human HepG2 whole cell lysate, <br> Lane 3: human Caco-2 whole cell lysate, <br> Lane 4: human U-937 whole cell lysate, <br> Lane 5: human Hela whole cell lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A3R1 antigen affinity purified polyclonal antibody (Catalog # A02427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A3R1 at approximately 50KD. The expected band size for SLC9A3R1 is at 39KD.
https://www.bosterbio.com/products/primary-antibodies/anti-kcc2-slc12a5-picoband-trade-antibody-a04629-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-1.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04629-1-slc12a5-primary-antibodies-ihc-testing-8.jpgAnti-KCC2/SLC12A5 Antibody Picoband®IHC analysis of KCC2/SLC12A5 using anti-KCC2/SLC12A5 antibody (A04629-1). <br>KCC2/SLC12A5 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCC2/SLC12A5 Antibody (A04629-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-2.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-3.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-4.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-5.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-IHC-testing-6.jpgAnti-KCC2/SLC12A5 Antibody Picoband® IHC analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1).
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SLC12A5 was detected in paraffin-embedded section of human sarcoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC12A5 Antibody (A04629-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04629-1-SLC12A5-primary-antibodies-WB-testing-7.jpgAnti-KCC2/SLC12A5 Antibody Picoband® Western blot analysis of SLC12A5 using anti-SLC12A5 antibody (A04629-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A5 antigen affinity purified polyclonal antibody (Catalog # A04629-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A5 at approximately 126KD. The expected band size for SLC12A5 is at 126KD.
https://www.bosterbio.com/products/primary-antibodies/anti-smarcad1-picoband-trade-antibody-a06049-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-IHC-testing-1.jpgAnti-SMARCAD1 Antibody Picoband® IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).
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SMARCAD1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-IHC-testing-2.jpgAnti-SMARCAD1 Antibody Picoband® IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).
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SMARCAD1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-IHC-testing-3.jpgAnti-SMARCAD1 Antibody Picoband® IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).
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SMARCAD1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-IHC-testing-4.jpgAnti-SMARCAD1 Antibody Picoband® IHC analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).
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SMARCAD1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-WB-testing-5.jpgAnti-SMARCAD1 Antibody Picoband® Western blot analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human U-87MG whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human THP-1 whole cell lysates, <br>
Lane 7: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCAD1 antigen affinity purified polyclonal antibody (Catalog # A06049-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCAD1 at approximately 150KD. The expected band size for SMARCAD1 is at 117KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06049-1-SMARCAD1-primary-antibodies-WB-testing-6.jpgAnti-SMARCAD1 Antibody Picoband® Western blot analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular tissue lysates, <br>
Lane 2: mouse thymus tissue lysates, <br>
Lane 3: mouse lung tissue lysates, <br>
Lane 4: mouse testicular tissue lysates, <br>
Lane 5: mouse kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCAD1 antigen affinity purified polyclonal antibody (Catalog # A06049-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCAD1 at approximately 150KD. The expected band size for SMARCAD1 is at 117KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06049-1-smarcad1-primary-antibodies-if-testing-7.jpgAnti-SMARCAD1 Antibody Picoband® IF analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1). <br>
SMARCAD1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SMARCAD1 Antibody (A06049-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06049-1-smarcad1-primary-antibodies-fcm-testing-8.jpgAnti-SMARCAD1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SMARCAD1 antibody (A06049-1). <br>Overlay histogram showing SiHa cells stained with A06049-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCAD1 Antibody (A06049-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06049-1-smarcad1-primary-antibodies-ip-testing-9.jpgAnti-SMARCAD1 Antibody Picoband® Immunoprecipitating SMARCAD1 in K562 whole cell lysate.<br>Western blot analysis of SMARCAD1 using anti-SMARCAD1 antibody (A06049-1).<br>Lane 1: K562 whole cell lysates (30ug)<br>Lane 2: Rabbit control IgG instead of anti-SMARCAD1 antibody in K562 whole cell lysate.<br>Lane 3: anti-SMARCAD1 antibody (2μg) + K562 whole cell lysate (500μg)<br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMARCAD1 antigen affinity purified polyclonal antibody (A06049-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SMARCAD1 at approximately 150 kDa. The expected band size for SMARCAD1 is at 117 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-trpc5-picoband-trade-antibody-a02701-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02701-TRPC5-primary-antibodies-WB-testing-1.jpgAnti-TRPC5 Antibody Picoband® Western blot analysis of TRPC5 using anti-TRPC5 antibody (A02701). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.
https://www.bosterbio.com/products/primary-antibodies/anti-wnt4-picoband-trade-antibody-a00879-boster.html2026-03-27T05:07:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00879-wnt4-primary-antibodies-wb-testing-1.jpgAnti-WNT4 Antibody Picoband®Western blot analysis of WNT4 using anti-WNT4 antibody (A00879). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT4 antigen affinity purified polyclonal antibody (A00879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WNT4 at approximately 45-50 kDa. The expected band size for WNT4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00879-wnt4-primary-antibodies-ihc-testing-1.jpgAnti-WNT4 Antibody Picoband®IHC analysis of WNT4 using anti-WNT4 antibody (A00879). <br>WNT4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WNT4 Antibody (A00879) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00879-wnt4-primary-antibodies-ihc-testing-2.jpgAnti-WNT4 Antibody Picoband®IHC analysis of WNT4 using anti-WNT4 antibody (A00879). <br>WNT4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WNT4 Antibody (A00879) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00879-wnt4-primary-antibodies-ihc-testing-3.jpgAnti-WNT4 Antibody Picoband®IHC analysis of WNT4 using anti-WNT4 antibody (A00879). <br>WNT4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WNT4 Antibody (A00879) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igg1-rabbit-monoclonal-antibody-clone-rm106-m04575-2-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-2-WB-test-result-image-1.jpgAnti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106
Western blot of nonreduced(-) and reduced(+)
mouse IgG1 (20ng/lane), using 0.2ug/mL of clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-2-ELISA-test-result-image-2.jpgAnti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106
ELISA of mouse immunoglobulins shows RM106
reacts to the Fc region of mouse IgG1; no cross
reactivity with IgG2a, IgG3, IgM, IgA, IgE, human
IgG, or rat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM106 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04575-2-elisa-test-result-image-3.jpgAnti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone#RM106ELISA of mouse immunoglobulins shows RM106
reacts to the Fc region of mouse IgG1; no cross
reactivity with IgG2a, IgG3, IgM, IgA, IgE, human
IgG, or rat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM106 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igg2a-kappa-rabbit-monoclonal-antibody-clone-rm107-m30946-1-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M30946-1-WB-test-result-image-1.jpgAnti-Mouse IgG2a kappa Rabbit Monoclonal Antibody, Clone#RM107 Western Blotting result<br>Western blot of nonreduced(-) and reduced(+) mouse IgG2a kappa(20 ng/lane), using 0.2ug/mL of clone RM107. This antibody only reacted to nonreduced Mouse IgG2a kappahttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M30946-1-ELISA-test-result-image-2.jpgAnti-Mouse IgG2a kappa Rabbit Monoclonal Antibody, Clone#RM107 ELISA result showing specificity<br>ELISA of mouse immunoglobulins shows RM107 reacted to the Fab region of mouse IgG2a; no cross reactivity with IgG2a kappa, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M30946-1-ELISA-test-result-image-3.jpgAnti-Mouse IgG2a kappa Rabbit Monoclonal Antibody, Clone#RM107 ELISA result showing specificity<br>A titer ELISA of mouse IgG2a kappa. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM107 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igg2b-rabbit-monoclonal-antibody-clone-rm108-m33092-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M33092-WB-test-result-image-1.jpgAnti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 Western Blotting result<br>Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of clone RM108. This antibody reacted to nonreduced IgG2b (~150 kDa) much stronger than the reduced Gamma 2b(~50 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M33092-ELISA-test-result-image-2.jpgAnti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 ELISA result showing specificity<br>ELISA of mouse immunoglobulins shows RM108 reacted to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M33092-ELISA-test-result-image-3.jpgAnti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 ELISA result showing specificity<br>A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igm-rabbit-monoclonal-antibody-clone-rm109-m07469-2-boster.html2026-03-24T05:23:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-2-ELISA-test-result-image-1.jpgAnti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109 ELISA result showing specificity<br>A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-2-ELISA-test-result-image-2.jpgAnti-Mouse IgM Ighm Rabbit Monoclonal Antibody, Clone#RM109 ELISA result showing specificity<br>ELISA of mouse immunoglobulins shows RM109 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM109 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-ig-kappa-light-chain-rabbit-monoclonal-antibody-clone-rm103-m05470-2-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-2-WB-test-result-image-1_1.jpgAnti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 Western Blotting result<br>Western blot of nonreduced(-) and reduced(+) mouse IgG1 and IgG1 (20ng/lane), using 0.2ug/mL of clone RM103. This antibody reacted to nonreduced IgG1 kappa (~150 kDa), and slightly react to reduced kappa light chain (~25 kDa)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-2-ELISA-test-result-image-2.jpgAnti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 ELISA result showing specificity<br>ELISA of mouse immunoglobulins shows RM103 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-2-ELISA-test-result-image-3_1.jpgAnti-Mouse Ig Kappa Light Chain Igkc Rabbit Monoclonal Antibody, Clone#RM103 ELISA result showing specificity<br>A titer ELISA of mouse IgG1 Kappa. The plate was coated with different amounts of mouse IgG1 Kappa. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-actin-rabbit-monoclonal-antibody-clone-rm112-m01263-2-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01263-2-WB-test-result-image-1.jpgAnti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 Western Blotting result<br>Western blot of A431 cells, using Clone RM112 at 1/1000 dilution. A -Actin band showed at the predicted MW (42 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-2-12957_2022_2722_fig2_html.pngAnti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112A ASCL2 expressed and B quantificated in different tissues. C IHC for ASCL2 (× 100). D ASCL2 expressed and E quantificated in different cell lines. Protein levels were normalized to β-actin (tumor vs. para-tumor, and MGC803 vs. other cells, n = 6, * P < 0.05) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-022-02722-y'>36008864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01263-2-ICC-test-result-image-2.pngAnti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 ICC result<br>Immunocytochemical staining of HeLa cells, using anti--Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue)
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-tubulin-rabbit-monoclonal-antibody-clone-rm113-m08382-2-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08382-2-WB-test-result-image-1.jpgAnti-alpha -Tubulin TUBA1B Rabbit Monoclonal Antibody, Clone#RM113 Western Blotting result<br>Western blot of A431 cells, using Clone RM113 at 1/1000 dilution. A -Tubulin band showed at the predicted MW (~50 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08382-2-ICC-test-result-image-2.pngAnti-alpha -Tubulin TUBA1B Rabbit Monoclonal Antibody, Clone#RM113 ICC result<br>Immunocytochemical staining of HeLa cells, using Anti--Tubulin Clone RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-gapdh-rabbit-monoclonal-antibody-clone-rm114-m00227-5-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00227-5-WB-test-result-image-1.jpgAnti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 Western Blotting result<br>Western blot of A431 cells, using Clone RM114 at 1/1000 dilution. A GAPDH band showed at the predicted MW (~36 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00227-5-ICC-test-result-image-2.pngAnti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 ICC result<br>Immunocytochemical staining of HeLa cells, using anti-GAPDH Clone RM114 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-gamma-heavy-chain-rabbit-monoclonal-antibody-clone-rm116-m04575-4-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-IHC-test-result-image-1.jpgAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 IHC result<br>Immunohistochemistry of human tonsil using anti-gamma heavy chain antibody RM116.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-ELISA-test-result-image-2.pngAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM116 reacted to the y1, y2, y3, y4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-ELISA-test-result-image-3.pngAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 ELISA result showing specificity<br>A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-ELISA-test-result-image-4.pngAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 ELISA result showing specificity<br>Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-ELISA-test-result-image-5.pngAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 ELISA result showing specificity<br>Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-4-ELISA-test-result-image-6.pngAnti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 ELISA result showing specificity<br>Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igg1-rabbit-monoclonal-antibody-clone-rm117-m04575-5-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-ELISA-test-result-image-1.pngAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 ELISA result showing specificity<br>Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-ELISA-test-result-image-2.pngAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 ELISA result showing specificity<br>Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains (k+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidinhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-ELISA-test-result-image-3.pngAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-ELISA-test-result-image-4.pngAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 ELISA result showing specificity<br>A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-ELISA-test-result-image-5.pngAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 ELISA result showing specificity<br>ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-5-IHC-test-result-image-6.jpgAnti-Human IgG1 IGHG1 Rabbit Monoclonal Antibody, Clone#RM117 IHC result<br>Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody RM117 at a 1:15,000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igg2-rabbit-monoclonal-antibody-clone-rm118-m07865-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07865-ELISA-test-result-image-1.pngAnti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 ELISA result showing specificity<br>Detection of human IgG2 in monkey serum. Sandwich ELISA using RM118 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07865-ELISA-test-result-image-2.pngAnti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 ELISA result showing specificity<br>Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidinhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07865-ELISA-test-result-image-3.pngAnti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM118 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07865-ELISA-test-result-image-4.pngAnti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 ELISA result showing specificity<br>A titer ELISA using RM118. The plate was coated with different amounts of human IgG2. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07865-ELISA-test-result-image-5.pngAnti-Human IgG2 IGHG2 Rabbit Monoclonal Antibody, Clone#RM118 ELISA result showing specificity<br>ELISA showing RM118 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM118 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igg3-rabbit-monoclonal-antibody-clone-rm119-m08374-1-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-ELISA-test-result-image-1.pngAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 ELISA result showing specificity<br>Detection of human IgG3 in monkey serum. Sandwich ELISA using RM119 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-ELISA-test-result-image-2.pngAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 ELISA result showing specificity<br>Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-ELISA-test-result-image-3.pngAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM119 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-ELISA-test-result-image-4.pngAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 ELISA result showing specificity<br>A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-ELISA-test-result-image-5.pngAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 ELISA result showing specificity<br>ELISA showing RM119 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM119 and a monkey IgG binding antibody (positive control) was used as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-IHC-test-result-image-6.jpgAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG3 rabbit monoclonal antibody (Clone RM119).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08374-1-IHC-test-result-image-7.jpgAnti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 IHC result<br>Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igg4-rabbit-monoclonal-antibody-clone-rm120-m11385-2-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-WB-test-result-image-1.pngAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 Western Blotting result<br>Western Blot of human, mouse, rat, and goat IgG shows RM120 reacted to human IgG4 both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and mouse, rat, or goat IgG.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-IHC-test-result-image-2.jpgAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 IHC result<br>Immunohistochemistry of human lymphoid tissue using anti-human IgG4 antibody RM120.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-ELISA-test-result-image-3.pngAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 ELISA result showing specificity<br>Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-ELISA-test-result-image-4.pngAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 ELISA result showing specificity<br>Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains ( + ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-ELISA-test-result-image-5.pngAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 ELISA result showing specificity<br>A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-ELISA-test-result-image-6.pngAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 ELISA result showing specificity<br>ELISA showing RM120 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM120 and a monkey IgG binding antibody (positive control) was used as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-2-IHC-test-result-image-7.jpgAnti-Human IgG4 IGHG4 Rabbit Monoclonal Antibody, Clone#RM120 IHC result<br>Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG4 antibody RM120 at a 1:50,000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igm-rabbit-monoclonal-antibody-clone-rm121-m07469-3-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-3-ELISA-test-result-image-1.pngAnti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 ELISA result showing specificity<br>Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-3-ELISA-test-result-image-2.pngAnti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 ELISA result showing specificity<br>Sandwich ELISA using RM121 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-3-ELISA-test-result-image-3.pngAnti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM121 only reacted to human IgM. No cross reactivity with IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07469-3-ELISA-test-result-image-4.pngAnti-Human IgM IGHM Rabbit Monoclonal Antibody, Clone#RM121 ELISA result showing specificity<br>A titer ELISA using RM121. The plate was coated with different amounts of human IgM. A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ige-rabbit-monoclonal-antibody-clone-rm122-m10533-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M10533-ELISA-test-result-image-1..pngAnti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 ELISA result showing specificity<br>Sandwich ELISA using RM122 as the capture antibody (25ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M10533-ELISA-test-result-image-2..pngAnti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM122 reacted to the IgE from human myeloma plasma and the IgE from human plasma. No cross reactivity with human IgG, IgM, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M10533-ELISA-test-result-image-3..pngAnti-Human IgE IGHE Rabbit Monoclonal Antibody, Clone#RM122 ELISA result showing specificity<br>A titer ELISA using RM122. The plate was coated with different amounts of human IgE (from plasma). A serial dilution of RM122 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igd-rabbit-monoclonal-antibody-clone-rm123-m05183-1-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05183-1-IHC-test-result-image-1.pngAnti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 IHC result<br>Immunohistochemistry of human lymphoid tissue using anti-human IgD antibody RM123https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05183-1-ELISA-test-result-image-2..pngAnti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 ELISA result showing specificity<br>Sandwich ELISA using RM123 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05183-1-ELISA-test-result-image-3..pngAnti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM123 reacted to the IgD from human plasma and the IgD from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05183-1-ELISA-test-result-image-4..pngAnti-Human IgD IGHD Rabbit Monoclonal Antibody, Clone#RM123 ELISA result showing specificity<br>A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-iga1-rabbit-monoclonal-antibody-clone-rm124-m07514-2-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-2-IHC-test-result-image-1.jpgAnti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 IHC result<br>Immunohistochemistry of human tonsil tissue using anti-human IgA1 antibody RM124https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-2-ELISA-test-result-image-2.pngAnti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 ELISA result showing specificity<br>Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-2-ELISA-test-result-image-3.pngAnti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 ELISA result showing specificity<br>Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-2-ELISA-test-result-image-4.pngAnti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM124 only reacted to human IgA1. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-2-ELISA-test-result-image-5.pngAnti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 ELISA result showing specificity<br>A titer ELISA using RM124. The plate was coated with different amounts of human IgA1. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-iga2-rabbit-monoclonal-antibody-clone-rm125-m11271-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11271-IHC-test-result-image-1.jpgAnti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 IHC result<br>Immunohistochemistry of human tonsil tissue using anti-human IgA2 antibody RM125https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11271-ELISA-test-result-image-2.pngAnti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 ELISA result showing specificity<br>Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidinhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11271-ELISA-test-result-image-3.pngAnti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 ELISA result showing specificity<br>Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidinhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11271-ELISA-test-result-image-4.pngAnti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM125 only reacted to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11271-ELISA-test-result-image-5.pngAnti-Human IgA2 IGHA2 Rabbit Monoclonal Antibody, Clone#RM125 ELISA result showing specificity<br>A titer ELISA using RM125. The plate was coated with different amounts of human IgA2. A serial dilution of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-kappa-light-chain-rabbit-monoclonal-antibody-clone-rm126-m05470-3-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-3-IHC-test-result-image-1.jpgAnti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig kappa light chain rabbit monoclonal antibody (Clone RM126).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-3-ELISA-test-result-image-2.pngAnti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 ELISA result showing specificity<br>ELISA showing RM126 reacts only to the kappa light chain of human immunoglobulins, and not to lambda light chain, mouse IgG, rat IgG, or goat IgGhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-3-ELISA-test-result-image-3.pngAnti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 ELISA result showing specificity<br>A titer ELISA using RM126. The plate was coated with different amounts of human IgG4. A serial dilution of RM126 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-3-ELISA-test-result-image-4.pngAnti-Human Kappa Light Chain IGKC Rabbit Monoclonal Antibody, Clone#RM126 ELISA result showing specificity<br>ELISA showing RM126 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM126 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-lambda-light-chain-rabbit-monoclonal-antibody-clone-rm127-m12334-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12334-IHC-test-result-image-1.jpgAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonal antibody (Clone RM127).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12334-ELISA-test-result-image-2.pngAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM127 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12334-ELISA-test-result-image-3.pngAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 ELISA result showing specificity<br>A titer ELISA using RM127. The plate was coated with different amounts of human IgG3. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12334-ELISA-test-result-image-4.pngAnti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone#RM127 ELISA result showing specificity<br>ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-iga-rabbit-monoclonal-antibody-clone-rm128-m07514-1-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-1-IHC-test-result-image-1.pngAnti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 IHC result<br>Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody RM128https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-1-ELISA-test-result-image-2.pngAnti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 ELISA result showing specificity<br>Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-1-ELISA-test-result-image-3.pngAnti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 ELISA result showing specificity<br>Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-1-ELISA-test-result-image-4.pngAnti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 ELISA result showing specificity<br>ELISA of human immunoglobulins shows RM128 reacted to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07514-1-ELISA-test-result-image-5.pngAnti-Human IgA IGHA1 Rabbit Monoclonal Antibody, Clone#RM128 ELISA result showing specificity<br>A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys14-rabbit-monoclonal-antibody-clone-rm130-m06819-1-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-1-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM130 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 14 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-1-Specificity-test-result-image-2..pngAnti-Acetyl-Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM130 Specificity Test result<br>RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-1-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM130 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys14) Rabbit mAb RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-1-ChIP-test-result-image-4.pngAnti-Acetyl-Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM130 ChIP result<br>ChIP performed on HeLa cells using H3K14ac antibody (RM130, 5ug). Real-time PCR was performed using primers specific to the gene indicated.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-1-Specificity-test-result-image-5..pngAnti-Acetyl-Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM130 Specificity Test result<br>The specific binding of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys4-rabbit-monoclonal-antibody-clone-rm135-m06819-4-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-4-WB-test-result-image-1.pngAnti-Dimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM135 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM135 at 0.025 ug/mL, showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-4-Specificity-test-result-image-2..pngAnti-Dimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM135 Specificity Test result<br>RM135 specifically reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1) or trimethylated Lysine 4 (K4me3), or other methylations in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-4-ICC-test-result-image-3.pngAnti-Dimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM135 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Dimethyl-Histone H3 (Lys4) Rabbit mAb RM135 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trimethyl-histone-h3-lys4-rabbit-monoclonal-antibody-clone-rm137-m06819-5-boster.html2026-03-24T05:23:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-5-WB-test-result-image-1.pngAnti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM137 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-5-Specificity-test-result-image-2.pngAnti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM137 Specificity Test result<br>RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-5-Dot-Blot-test-result-image-3.pngAnti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM137 Dot Blot result<br>A Peptide dotblot shows RM137 only reacts to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-monomethyl-histone-h3-lys4-rabbit-monoclonal-antibody-clone-rm140-m06819-3-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-3-WB-test-result-image-1.pngAnti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM140 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-3-Specificity-test-result-image-2..pngAnti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140 Specificity Test result<br>RM140 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-3-ICC-test-result-image-3.pngAnti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140 ICC result<br>Immunocytochemistry of HeLa cells, using Monomethyl-Histone H3 (Lys4) Rabbit mAb RM140 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-3-ChIP-test-result-image-4.pngAnti-Monomethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM140 ChIP result<br>ChIP performed on HeLa cells using H3K4me1 antibody (RM140, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys36-rabbit-monoclonal-antibody-clone-rm141-m06819-6-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-6-WB-test-result-image-1.pngAnti-Dimethyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM141 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 (K36me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-6-Specificity-test-result-image-2.pngAnti-Dimethyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM141 Specificity Test result<br>RM141 specifically reacts to Histone H3 dimethylated at Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-monomethyl-histone-h3-lys79-rabbit-monoclonal-antibody-clone-rm147-m06819-10-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-10-WB-test-result-image-1.pngAnti-Monomethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM147 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM147 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 79 (K79me1) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-10-Specificity-test-result-image-2.pngAnti-Monomethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM147 Specificity Test result<br>RM147 specifically reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated Lysine 79 (K79me2) or trimethylated Lysine 79 (K79me3), or other methylations in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-10-ChIP-test-result-image-3.pngAnti-Monomethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM147 ChIP result<br>ChIP performed on HeLa cells using H3K79me1 antibody (RM147, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys36-rabbit-monoclonal-antibody-clone-rm154-m06819-8-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-8-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM154 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 36 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-8-Specificity-test-result-image-2..pngAnti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 Specificity Test result<br>RM154 specifically reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-8-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM154 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys36) Rabbit mAb RM154 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trimethyl-histone-h3-lys36-rabbit-monoclonal-antibody-clone-rm155-m06819-7-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-7-WB-test-result-image-1.pngAnti-Trimethyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM155 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2 ug/mL, showed a band of histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-7-Specificity-test-result-image-2.pngAnti-Trimethyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM155 Specificity Test result<br>RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-7-ChIP-test-result-image-3.pngAnti-Trimethyl-Histone H3 (Lys36) H3F3A Rabbit Monoclonal Antibody, Clone#RM155 ChIP result<br>ChIP performed on HeLa cells using H3K36me3 antibody (RM155, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys79-rabbit-monoclonal-antibody-clone-rm156-m06819-9-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-9-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM156 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM156 at 1 ug/mL, showed a band of Histone H3 acetylated at Lysine 79 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-9-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM156 Specificity Test result<br>RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-9-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM156 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys79) Rabbit mAb RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-9-ELISA-test-result-image-4.pngAnti-Acetyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM156 ELISA result showing specificity<br>Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 ug/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 ug/mL) as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-9-ChIP-test-result-image-5.pngAnti-Acetyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM156 ChIP result<br>ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K79Ac antibody (RM156, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trimethyl-histone-h3-lys79-rabbit-monoclonal-antibody-clone-rm157-m06819-19-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-19-WB-test-result-image-1.pngAnti-Trimethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM157 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 79 (K79me3) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-19-Specificity-test-result-image-2.pngAnti-Trimethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM157 Specificity Test result<br>RM157 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-19-Dot-Blot-test-result-image-3.pngAnti-Trimethyl-Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM157 Dot Blot result<br>A Peptide dotblot shows RM157 only reacts to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity with nonmodified Lysine 79 (H3K79Ctrl), monomethylated Lysine 79 (K79me1) or dimethylated Lysine 79 (K79me2).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys9-rabbit-monoclonal-antibody-clone-rm161-m06819-11-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM161 at 0.25 ug/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 Specificity Test result<br>RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-IHC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 IHC result<br>Immunohistochemistry staining of HepG2 cell using anti-Acetyl-Histone H3 (Lys9) antibody, RM161.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-ICC-test-result-image-4.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-ELISA-test-result-image-5.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 ELISA result showing specificity<br>Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 ug/mL) as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-11-ChIP-test-result-image-6.pngAnti-Acetyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM161 ChIP result<br>ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys18-rabbit-monoclonal-antibody-clone-rm166-m06819-12-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM166 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 18 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 Specificity Test result<br>RM166 specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-IHC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 IHC result<br>Immunohistochemistry staining of HepG2 cells using anti-Acetyl-Histone H3 (Lys18) antibody, RM166.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-ICC-test-result-image-4.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys18) Rabbit mAb RM166(red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-ELISA-test-result-image-5.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 ELISA result showing specificity<br>Sandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K18ac (RM166, 2 ug/mL) as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-12-ChIP-test-result-image-6.pngAnti-Acetyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM166 ChIP result<br>ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K18ac antibody (RM166, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys27-rabbit-monoclonal-antibody-clone-rm172-m06819-13-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-13-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM172 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM172 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 27 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-13-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM172 Specificity Test result<br>RM172 specifically reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-13-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM172 ICC result<br>Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys27) Rabbit mAb RM172 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h3-pan-rabbit-monoclonal-antibody-clone-rm188-m06819-28-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-28-WB-test-result-image-1.pngAnti-Histone H3 pan H3F3A Rabbit Monoclonal Antibody, Clone#RM188 Western Blotting result<br>Western Blot of recombinant Histone H3.3 and Histone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM188 at 0.025 ug/mL, showed a band of recombinant H3.1 and H3.3 proteins, and total histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-28-ICC-test-result-image-2.pngAnti-Histone H3 pan H3F3A Rabbit Monoclonal Antibody, Clone#RM188 ICC result<br>Immunocytochemistry of HeLa cells, using Histone H3 Rabbit mAb RM188 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-28-ELISA-test-result-image-3.pngAnti-Histone H3 pan H3F3A Rabbit Monoclonal Antibody, Clone#RM188 ELISA result showing specificity<br>Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161, 1 ug/mL) as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-28-ELISA-test-result-image-4.pngAnti-Histone H3 pan H3F3A Rabbit Monoclonal Antibody, Clone#RM188 ELISA result showing specificity<br>Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 ug/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 ug/mL) as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-28-Specificity-test-result-image-5.pngAnti-Histone H3 pan H3F3A Rabbit Monoclonal Antibody, Clone#RM188 Specificity Test result<br>RM188 reacts to both Histone H3.1 and Histone H3.3.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h3-3-rabbit-monoclonal-antibody-clone-rm190-m06819-29-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-29-WB-test-result-image-1.pngAnti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody, Clone#RM190 Western Blotting result<br>Western Blot of HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-29-Specificity-test-result-image-2.pngAnti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody, Clone#RM190 Specificity Test result<br>RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys9-rabbit-monoclonal-antibody-clone-rm151-m06819-14-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-14-WB-test-result-image-1.pngAnti-Dimethyl Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM151 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0. 5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-14-Specificity-test-result-image-2.pngAnti-Dimethyl Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM151 Specificity Test result<br>RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-14-ICC-test-result-image-3.pngAnti-Dimethyl Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM151 ICC result<br>Immunocytochemistry of HeLa cells, using Dimethyl-Histone H3 (Lys9) Rabbit mAb RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-14-ChIP-test-result-image-4.pngAnti-Dimethyl Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM151 ChIP result<br>ChIP performed on HeLa cells using H3K9me2 antibody (RM151, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys14-rabbit-monoclonal-antibody-clone-rm165-m06819-15-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-15-WB-test-result-image-1.pngAnti-Dimethyl Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM165 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM165 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 14 (K14me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-15-Specificity-test-result-image-2.pngAnti-Dimethyl Histone H3 (Lys14) H3F3A Rabbit Monoclonal Antibody, Clone#RM165 Specificity Test result<br>RM165 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with non-modified Lysine 14 (H3k14), monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trimethyl-histone-h3-lys27-rabbit-monoclonal-antibody-clone-rm175-m06819-18-boster.html2026-03-24T05:23:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-18-WB-test-result-image-1.pngAnti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 ug/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-18-Specificity-test-result-image-2.pngAnti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 Specificity Test result<br>RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-18-IHC-test-result-image-3.pngAnti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 IHC result<br>Immunohistochemistry staining of HepG2 cell using anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h3-lys4-rabbit-monoclonal-antibody-clone-rm149-m06819-16-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-16-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM149 Western Blotting result<br>Western Blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate, using RM149 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 4 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-16-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM149 Specificity Test result<br>RM149 specifically reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-16-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM149 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys4) Rabbit mAb RM149 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-monomethyl-histone-h3-lys9-rabbit-monoclonal-antibody-clone-rm150-m06819-17-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-17-WB-test-result-image-1.pngAnti-Monomethyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM150 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 9 (K4me1) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-17-Specificity-test-result-image-2.pngAnti-Monomethyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM150 Specificity Test result<br>RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-17-ICC-test-result-image-3.pngAnti-Monomethyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM150 ICC result<br>Immunocytochemistry of HeLa cells treated withsodium butyrate, using Monomethyl-Histone H3(Lys9) Rabbit mAb RM150 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-17-ICC-test-result-image-4.pngAnti-Monomethyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM150 ICC result<br>Immunocytochemistry of HeLa cells treated withsodium butyrate, using Monomethyl-Histone H3(Lys9) Rabbit mAb RM150 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys79-rabbit-monoclonal-antibody-clone-rm181-m06819-23-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-23-WB-test-result-image-1.pngAnti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-23-Specificity-test-result-image-2.pngAnti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 Specificity Test result<br>RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-23-IHC-test-result-image-3.pngAnti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 IHC result<br>Immunohistochemistry staining of HepG2 cell using anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-23-ChIP-test-result-image-4.pngAnti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 ChIP result<br>ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h3-pser10-rabbit-monoclonal-antibody-clone-rm163-p06819-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-ICC-test-result-image-1.pngAnti-Phospho-Histone H3 (pSer10) H3F3A Rabbit Monoclonal Antibody, Clone#RM163 ICC result<br>Immunocytochemistry of HeLa cells using Phospho-Histone H3(Ser10) Rabbit mAb RM163 (red). Actin filaments have beenlabeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-ICC-test-result-image-2.pngAnti-Phospho-Histone H3 (pSer10) H3F3A Rabbit Monoclonal Antibody, Clone#RM163 ICC result<br>Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Ser10) Rabbit mAb RM163 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h3-thr3-rabbit-monoclonal-antibody-clone-rm159-p06819-1-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-1-ICC-test-result-image-1.pngAnti-Phospho-Histone H3 (Thr3) H3F3A Rabbit Monoclonal Antibody, Clone#RM159 ICC result<br>Immunocytochemistry of HeLa cells using Phospho-Histone H3(Thr3) Rabbit mAb RM159 (red). Actin filaments have beenlabeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-1-ICC-test-result-image-2.pngAnti-Phospho-Histone H3 (Thr3) H3F3A Rabbit Monoclonal Antibody, Clone#RM159 ICC result<br>Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-1-ICC-test-result-image-3.pngAnti-Phospho-Histone H3 (Thr3) H3F3A Rabbit Monoclonal Antibody, Clone#RM159 ICC result<br>Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h3-thr11-rabbit-monoclonal-antibody-clone-rm164-p06819-3-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-3-WB-test-result-image-1.pngAnti-Phospho-Histone H3 (Thr11) H3F3A Rabbit Monoclonal Antibody, Clone#RM164 Western Blotting result<br>Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM164 at 0.5 ug/mL, showed a band of Histone H3 phosphorylated at threonine 11 in HeLa cells.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys18-rabbit-monoclonal-antibody-clone-rm168-m06819-20-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-20-WB-test-result-image-1.pngAnti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-20-Specificity-test-result-image-2.pngAnti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 Specificity Test result<br>RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-20-Dot-Blot-test-result-image-3.pngAnti-Dimethyl Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM168 Dot Blot result<br>A Peptide dotblot shows RM168 only reacts to Histone H3 dimethyl-Lysine 18 (K18me2). No cross reactivity with nonmodified Lysine 18 (K18Ctrl), monomethylated Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2), or dimethylated Lysine 36 (K36me2).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-monomethyl-histone-h3-lys18-rabbit-monoclonal-antibody-clone-rm167-m06819-21-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-21-WB-test-result-image-1.pngAnti-Monomethyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM167 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM167 at 1 ug/mL, showed a band of histone H3 monomethy-lated at Lysine 18 (K18me1) in HeLa cells. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-21-WB-test-result-image-2.pngAnti-Monomethyl-Histone H3 (Lys18) H3F3A Rabbit Monoclonal Antibody, Clone#RM167 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM167 at 1 ug/mL, showed a band of histone H3 monomethy-lated at Lysine 18 (K18me1) in HeLa cells.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dimethyl-histone-h3-lys23-rabbit-monoclonal-antibody-clone-rm171-m06819-22-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-22-WB-test-result-image-1.pngAnti-Dimethyl Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM171 Western Blotting result<br>Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM171 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 23 (K23me2) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-22-Specificity-test-result-image-2.pngAnti-Dimethyl Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM171 Specificity Test result<br>RM171 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-22-Dot-Blot-test-result-image-3.pngAnti-Dimethyl Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM171 Dot Blot result<br>A Peptide dotblot shows RM171 only reacts to Histone H3 dimethyl-Lysine 23 (K23me2). No cross reactivity with nonmodified Lysine 23 (K23Ctrl), monomethylated Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-acetyl-histone-h3-lys56-rabbit-monoclonal-antibody-clone-rm179-m06819-24-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-24-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM179 Western Blotting result<br>Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant histone H3.3, using RM179 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 56 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-24-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM179 Specificity Test result<br>RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-24-IHC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM179 IHC result<br>Immunohistochemistry staining of HepG2 cell using anti-Acetyl-Histone H3 (Lys56) antibody, RM179.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-24-ICC-test-result-image-4.pngAnti-Acetyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM179 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h4-ser1-rabbit-monoclonal-antibody-clone-rm194-p14495-boster.html2026-03-24T05:23:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/1/P14495-Specificity-test-result-image-1.pngAnti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 Specificity Test result<br>RM194 specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/1/P14495-ICC-test-result-image-2.pngAnti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/1/P14495-ICC-test-result-image-3.pngAnti-Phospho-Histone H4 (Ser1) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM194 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-monomethyl-histone-h4-arg3-rabbit-monoclonal-antibody-clone-rm195-m14495-5-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-5-WB-test-result-image-1.pngAnti-Monomethyl-Histone H4 (Arg3) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM195 Western Blotting result<br>Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-5-Specificity-test-result-image-2.pngAnti-Monomethyl-Histone H4 (Arg3) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM195 Specificity Test result<br>RM195 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-5-ICC-test-result-image-3.pngAnti-Monomethyl-Histone H4 (Arg3) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM195 ICC result<br>Immunocytochemical staining of HeLa cells using anti-Monomethyl-Histone H4 (Arg3) Antibody (Clone RM195) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h4-lys5-rabbit-monoclonal-antibody-clone-rm199-m14495-6-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-6-WB-test-result-image-1.pngAnti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 Western Blotting result<br>Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, and (2) recombinant Histone H4. Using RM199 at 0.2 ug/mL, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-6-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 Specificity Test result<br>RM199 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-6-ICC-test-result-image-3.pngAnti-Acetyl-Histone H4 (Lys5) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM199 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (Clone RM199) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h4-lys8-rabbit-monoclonal-antibody-clone-rm201-m14495-7-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-7-WB-test-result-image-1.pngAnti-Acetyl-Histone H4 (Lys8) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM201 Western Blotting result<br>Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using RM201 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-7-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H4 (Lys8) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM201 Specificity Test result<br>RM201 specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-7-ICC-test-result-image-3.pngAnti-Acetyl-Histone H4 (Lys8) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM201 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody (Clone RM201) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h4-lys16-rabbit-monoclonal-antibody-clone-rm204-m14495-8-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-8-WB-test-result-image-1.pngAnti-Acetyl-Histone H4 (Lys16) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM204 Western Blotting result<br>Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-8-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H4 (Lys16) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM204 Specificity Test result<br>RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h4-lys20-rabbit-monoclonal-antibody-clone-rm205-m14495-9-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-9-WB-test-result-image-1.pngAnti-Acetyl-Histone H4 (Lys20) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM205 Western Blotting result<br>Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM205 at 1 ug/mL, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-9-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H4 (Lys20) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM205 Specificity Test result<br>RM205 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-9-ICC-test-result-image-3.pngAnti-Acetyl-Histone H4 (Lys20) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM205 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody (Clone RM205) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-histone-h3-unmodified-lys4-rabbit-monoclonal-antibody-clone-rm186-m08865-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08865-WB-test-result-image-1.pngAnti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 Western Blotting result<br>Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08865-ICC-test-result-image-2.pngAnti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 ICC result<br>Immunocytochemical staining of HeLa cells using Anti-Histone H3 (Unmodified Lys4) (Clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08865-Specificity-test-result-image-3.pngAnti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone#RM186 Specificity Test result<br>RM186 specifically recognizes Histone H3 that is unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-acetyl-histone-h3-lys23-rabbit-monoclonal-antibody-clone-rm169-m06819-25-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-25-WB-test-result-image-1.pngAnti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 Western Blotting result<br>Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant histone H3.3 (3), using RM169 at 1 ug/mL, showed a band of histone H3 acetylated at Lysine 23 in treated HeLa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-25-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 Specificity Test result<br>RM169 specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-25-ICC-test-result-image-3.pngAnti-Acetyl-Histone H3 (Lys23) H3F3A Rabbit Monoclonal Antibody, Clone#RM169 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Lys23) (Clone RM169) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h4-lys12-rabbit-monoclonal-antibody-clone-rm202-m14495-10-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-10-WB-test-result-image-1.pngAnti-Acetyl-Histone H4 (Lys12) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM202 Western Blotting result<br>Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using RM202 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-10-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H4 (Lys12) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM202 Specificity Test result<br>RM202 specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 12 (K126 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-10-ICC-test-result-image-3.pngAnti-Acetyl-Histone H4 (Lys12) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM202 ICC result<br>Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody (Clone RM202) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h4-rabbit-monoclonal-antibody-clone-rm212-m14495-12-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-12-ICC-test-result-image-1.pngAnti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody, Clone#RM212 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Histone H4 Rabbit mAb RM212 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-12-ICC-test-result-image-2.pngAnti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody, Clone#RM212 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Histone H4 Rabbit mAb RM212 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-12-ICC-test-result-image-3.pngAnti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody, Clone#RM212 ICC result<br>Immunocytochemistry of HeLa cells, using Histone H4 Rabbit mAb RM212 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h2ax-rabbit-monoclonal-antibody-clone-rm214-m00241-2-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00241-2-WB-test-result-image-1.pngAnti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 Western Blotting result<br>Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00241-2-ICC-test-result-image-2.pngAnti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 ICC result<br>Immunocytochemistry of HeLa cells, using Histone H2AX Rabbit mAb RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h2az-rabbit-monoclonal-antibody-clone-rm215-m04816-2-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-2-WB-test-result-image-1.pngAnti-Histone H2AZ H2AFZ Rabbit Monoclonal Antibody, Clone#RM215 Western Blotting result<br>Western Blot of HeLa, HEK293, A375, SK-MEL-2 and A431 whole cell lysates, using RM215 at 0.5 ug/mL, showed the endogenous Histone H2AZ HeLa, HEK293, A375, SK-MEL-2 and A431 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-2-ICC-test-result-image-2.pngAnti-Histone H2AZ H2AFZ Rabbit Monoclonal Antibody, Clone#RM215 ICC result<br>Immunocytochemistry of HeLa cells, using Histone H2AZ Rabbit mAb RM215 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h2a-h4-ser1-rabbit-monoclonal-antibody-clone-rm216-p00241-boster.html2026-03-24T05:24:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00241-ICC-test-result-image-1.pngAnti-Phospho-Histone H2A/H4 (Ser1) H2AFX Rabbit Monoclonal Antibody, Clone#RM216 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00241-ICC-test-result-image-2.pngAnti-Phospho-Histone H2A/H4 (Ser1) H2AFX Rabbit Monoclonal Antibody, Clone#RM216 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00241-ICC-test-result-image-3.pngAnti-Phospho-Histone H2A/H4 (Ser1) H2AFX Rabbit Monoclonal Antibody, Clone#RM216 ICC result<br>Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-phospho-histone-h3-thr6-rabbit-monoclonal-antibody-clone-rm160-p06819-2-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-2-WB-test-result-image-1.pngAnti-Phospho-Histone H3 (Thr6) H3F3A Rabbit Monoclonal Antibody, Clone#RM160 Western Blotting result<br>Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM160 at 0.1 ug/mL, showed a band of Histone H3 phospho-rylated at threonine 6 in HeLa cells.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-monomethyl-histone-h3-lys56-rabbit-monoclonal-antibody-clone-rm180-m06819-26-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-26-WB-test-result-image-1.pngAnti-Monomethyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM180 Western Blotting result<br>Western Blot of acid extracts of HeLa cells (1) and recombinant histone H3.3 (2), using RM180 at 1 ug/mL, showed a band of histone H3 monomethylated at Lysine 56 (K56me1) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-26-Specificity-test-result-image-2.pngAnti-Monomethyl-Histone H3 (Lys56) H3F3A Rabbit Monoclonal Antibody, Clone#RM180 Specificity Test result<br>RM180 specifically reacts to Histone H3 monomethylated at Lysine 56 (K56me1). No cross reactivity with nonmodified Lysine 56 or other methylations in histone H3.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trimethyl-histone-h4-lys20-rabbit-monoclonal-antibody-clone-rm208-m14495-11-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-11-Specificity-test-result-image-1.pngAnti-Trimethyl-Histone H4 (Lys20) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM208 Specificity Test result<br>RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M14495-11-Specificity-test-result-image-2.pngAnti-Trimethyl-Histone H4 (Lys20) HIST1H4A Rabbit Monoclonal Antibody, Clone#RM208 Specificity Test result<br>RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-trimethyl-phospho-histone-h3-lys9-ser10-rabbit-monoclonal-antibody-clone-rm162-p06819-4-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-4-WB-test-result-image-1.pngAnti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) H3F3A Rabbit Monoclonal Antibody, Clone#RM162 Western Blotting result<br>Western Blot of acid extracts of HeLa cells (1) and recombinant histone H3.3 (2), using RM162 at 0. 01 ug/mL, showed a band of histone H3 modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p) in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P06819-4-Specificity-test-result-image-2.pngAnti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) H3F3A Rabbit Monoclonal Antibody, Clone#RM162 Specificity Test result<br>RM162 specifically reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p). No cross reactivity with non-modified Lysine 9/ Serine 10, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igg2a-rabbit-monoclonal-antibody-clone-rm219-m30946-2-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M30946-2-WB-test-result-image-1.pngAnti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219Western blot of nonreduced(-) and reduced(+)
mouse IgG2a, using 0.5ug/mL of clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/3/M30946-2-ELISA-test-result-image-2.pngAnti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219ELISA of mouse immunoglobulins shows RM219
reacts to the Fc region of mouse IgG2a; no cross
reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30946-2-elisa-test-result-image-3.pngAnti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-igg4-fc-rabbit-monoclonal-antibody-clone-rm217-m11385-3-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-3-Specificity-test-result-image-1.pngAnti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 Specificity Test result<br>RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-3-ELISA-test-result-image-2.pngAnti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 ELISA result showing specificity<br>Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-3-ELISA-test-result-image-3.pngAnti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 ELISA result showing specificity<br>Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11385-3-ELISA-test-result-image-4.pngAnti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 ELISA result showing specificity<br>A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h2a-z-lys4-rabbit-monoclonal-antibody-clone-rm221-m04816-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-Specificity-test-result-image-1.pngAnti-Acetyl-Histone H2A.Z (Lys4) H2AFZ Rabbit Monoclonal Antibody, Clone#RM221 Specificity Test result<br>RM221 specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-ICC-test-result-image-2.pngAnti-Acetyl-Histone H2A.Z (Lys4) H2AFZ Rabbit Monoclonal Antibody, Clone#RM221 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone RM221) (red). Actin filaments have been labeled with fluorescein phalloidin (green). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-ICC-test-result-image-3.pngAnti-Acetyl-Histone H2A.Z (Lys4) H2AFZ Rabbit Monoclonal Antibody, Clone#RM221 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone RM221) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h2a-z-lys7-rabbit-monoclonal-antibody-clone-rm222-m04816-1-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-1-Specificity-test-result-image-1.pngAnti-Acetyl-Histone H2A.Z (Lys7) H2AFZ Rabbit Monoclonal Antibody, Clone#RM222 Specificity Test result<br>RM222 specifically reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-1-ICC-test-result-image-2.pngAnti-Acetyl-Histone H2A.Z (Lys7) H2AFZ Rabbit Monoclonal Antibody, Clone#RM222 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys7) Rabbit Monoclonal Antibody (clone RM222) (red). Actin filaments have been labeled with fluorescein phalloidin (green). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04816-1-ICC-test-result-image-3.pngAnti-Acetyl-Histone H2A.Z (Lys7) H2AFZ Rabbit Monoclonal Antibody, Clone#RM222 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys7) Rabbit Monoclonal Antibody (clone RM222) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-5-rabbit-monoclonal-antibody-clone-rm226-m00398-3-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00398-3-WB-test-result-image-1.pngAnti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 Western Blotting result<br>Western Blot of A431 cell lysates using RM226 using Anti-CK-5 rabbit monoclonal antibody (Clone RM226) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00398-3-IHC-test-result-image-2.pngAnti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00398-3-IHC-test-result-image-3.pngAnti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-her2-rabbit-monoclonal-antibody-clone-rm228-m00010-2-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00010-2-WB-test-result-image-1.pngAnti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 Western Blotting result<br>Western Blot of MCF-7, PC3, Jurkat, and A375 cell lysates using RM228, showed a band of Her2 expressed only in MCF-7 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00010-2-IHC-test-result-image-2.pngAnti-HER2 Rabbit Monoclonal Antibody, Clone#RM228 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h2a-rabbit-monoclonal-antibody-clone-rm225-m16777-4-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M16777-4-WB-test-result-image-1.pngAnti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225 Western Blotting result<br>Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM225 at 0.5 ug/mLhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M16777-4-ICC-test-result-image-2.pngAnti-Histone H2A HIST1H2AB Rabbit Monoclonal Antibody, Clone#RM225 ICC result<br>Immunocytochemistry of HeLa cells, using Histone H2A Rabbit mAb RM225 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h2b-rabbit-monoclonal-antibody-clone-rm230-m08989-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08989-WB-test-result-image-1.pngAnti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230 Western Blotting result<br>Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mLhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08989-ICC-test-result-image-2.pngAnti-Histone H2B Rabbit Monoclonal Antibody, Clone#RM230 ICC result<br>Immunocytochemical staining of HeLa cells, using Histone H2B Rabbit mAb RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-histone-h2b-lys20-rabbit-monoclonal-antibody-clone-rm235-m12718-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12718-WB-test-result-image-1.pngAnti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 Western Blotting result<br>Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM235 at 0.5 ug/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12718-Specificity-test-result-image-2.pngAnti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 Specificity Test result<br>RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M12718-ICC-test-result-image-3.pngAnti-Acetyl-Histone H2B (Lys20) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM235 ICC result<br>Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (Clone RM235) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-histone-h2b-ser14-rabbit-monoclonal-antibody-clone-rm238-p12718-1-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/1/P12718-1-WB-test-result-image-1.pngAnti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238 Western Blotting result<br>Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/1/P12718-1-ICC-test-result-image-2.pngAnti-Phospho-Histone H2B (Ser14) HIST1H2BB Rabbit Monoclonal Antibody, Clone#RM238 ICC result<br>Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acetyl-coa-carboxylase-rabbit-monoclonal-antibody-clone-rm232-m01802-1-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01802-1-WB-test-result-image-1.pngAnti-Acetyl-CoA Carboxylase ACACA Rabbit Monoclonal Antibody, Clone#RM232 Western Blotting result<br>Western Blot of A431 cell lysates using Anti-Acetyl CoA Carboxylase 1 (RM232) at a 1:1,000 dilution, showed a band of Acetyl CoA Carboxylase 1 (~260 kDa) expressed in A431 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01802-1-IHC-test-result-image-2.pngAnti-Acetyl-CoA Carboxylase ACACA Rabbit Monoclonal Antibody, Clone#RM232 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human heart tissue sections using Anti-Acetyl CoA Carboxylase 1 RM232 at a 1:300 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-rsk1-thr359-ser363-rabbit-monoclonal-antibody-clone-rm233-p01058-3-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P01058-3-WB-test-result-image-1.pngAnti-Phospho-Rsk1 (Thr359/Ser363) RPS6KA1 Rabbit Monoclonal Antibody, Clone#RM233 Western Blotting result<br>Western Blot of A431 cells treated (+) or nontreated (-) with EGF, using Anti-phospho-Rsk1 (Thr359/Ser363) RM233 at a 1:1000 dilution, showed a band of phosphorylated Rsk1 only in EGF treated A431 cells .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P01058-3-IHC-test-result-image-2.pngAnti-Phospho-Rsk1 (Thr359/Ser363) RPS6KA1 Rabbit Monoclonal Antibody, Clone#RM233 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human cerebral cortex tissue sections using Anti-phospho-Rsk1 (Thr359/Ser363) RM233 at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-core-histone-macro-h2a-1-h2afy-rabbit-monoclonal-antibody-clone-rm248-m04635-1-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04635-1-WB-test-result-image-1.pngAnti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 Western Blotting result<br>Western Blot of acid extracts from K562 cells, using RM248 at 1 ug/mLhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04635-1-ICC-test-result-image-2.pngAnti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 ICC result<br>Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hif-1-alpha-hif1a-rabbit-monoclonal-antibody-clone-rm242-m00013-1-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00013-1-WB-test-result-image-1.pngAnti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242 Western Blotting result<br>Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt(II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expression was induced by CoCl2 in Jurkat cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00013-1-IHC-test-result-image-2.pngAnti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d1-ccnd1-rabbit-monoclonal-antibody-clone-rm241-m00149-3-boster.html2026-03-24T05:24:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00149-3-WB-test-result-image-1.pngAnti-Cyclin D1 (CCND1) Rabbit Monoclonal Antibody, Clone#RM241 Western Blotting result<br>Western Blot of HeLa cell lysates using Anti-Cyclin D1 RM241 at a 1:1000 dilution, showed a band of Cyclin D1 (~34 kDa) expressed in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00149-3-IHC-test-result-image-2.pngAnti-Cyclin D1 (CCND1) Rabbit Monoclonal Antibody, Clone#RM241 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Cyclin D1 RM241 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e-cadherin-cdh1-rabbit-monoclonal-antibody-clone-rm244-m00063-3-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00063-3-WB-test-result-image-1.pngAnti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244 Western Blotting result<br>Western Blot of MCF-7 cell lysates using Anti-E-cadherin RM244 at a 1:1000 dilution, showed a band of E-cadherin (~120 kDa) expressed in MCF-7 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00063-3-IHC-test-result-image-2.pngAnti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-E-cadherin RM244 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmin-desm-rabbit-monoclonal-antibody-clone-rm234-m01948-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-2-WB-test-result-image-1.pngAnti-Desmin (DESM) Rabbit Monoclonal Antibody, Clone#RM234 Western Blotting result<br>Western Blot of mouse heart tissue lysates using Anti-Desmin RM234 at a 1:1000 dilution, showed a band of Desmin (~54 kDa) expressed in mouse heart.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-2-IHC-test-result-image-2.pngAnti-Desmin (DESM) Rabbit Monoclonal Antibody, Clone#RM234 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human heart tissue sections using Anti-Desmin RM234 at a 1:4000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p38-mapk-phospho-pt180-py182-rabbit-monoclonal-antibody-clone-rm243-p00176-1-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00176-1-WB-test-result-image-1.pngAnti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243
Western Blot of HeLa cell lysates, treated or non-
treated with anisomycin using Anti-Phospho-p38
MAPK (Thr180/Tyr182) RM243 at a 1:2000 dilution,
showed a band of Phospho-p38 MAPK (~38 kDa)
in anisomycin-treated HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00176-1-IHC-test-result-image-2.pngAnti-p38 MAPK Phospho (pT180/pY182) MAPK14 Rabbit Monoclonal Antibody, Clone#RM243Immunohistochemical staining of formalin fixed and
paraffin embedded human breast cancer tissue
sections using Anti-Phospho-p38 MAPK (Thr180/
Tyr182) RM243 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p38-mapk-rabbit-monoclonal-antibody-clone-rm245-m00176-3-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00176-3-WB-test-result-image-1.pngAnti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-p38 MAPK RM245 at a 1:1000 dilution, showed a band of p38 MAPK (~38 kDa) expressed in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00176-3-IHC-test-result-image-2.pngAnti-p38 MAPK MAPK14 Rabbit Monoclonal Antibody, Clone#RM245 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-p38 MAPK RM245 at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfap-rabbit-monoclonal-antibody-clone-rm246-m00213-7-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00213-7-WB-test-result-image-1.pngAnti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 Western Blotting result<br>Western Blot of mouse brain tissue lysate, using Anti-GFAP RM246 at a 1:2500 dilution, showed GFAP (~50 kDa) expression in mouse brain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00213-7-IHC-test-result-image-2.pngAnti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-GFAP RM246 at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd31-pecam-1-rabbit-monoclonal-antibody-clone-rm247-m01513-3-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01513-3-WB-test-result-image-1.pngAnti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 Western Blotting result<br>Western Blot of Jurkat cell lysate, using Anti-CD31 RM247 at a 1:1000 dilution, showed CD31 (~130 kDa) expression in Jurkat cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01513-3-IHC-test-result-image-2.pngAnti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD31 RM247 at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd146-rabbit-monoclonal-antibody-clone-rm249-m01683-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01683-2-WB-test-result-image-1.pngAnti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 Western Blotting result<br>Western Blot of A375 cell lysate, using Anti-CD146 RM249 at a 1:1000 dilution, showed CD146 (~110 kDa) expression in A375 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01683-2-IHC-test-result-image-2.pngAnti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD146 RM249 at a 1:400 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-3-rabbit-monoclonal-antibody-clone-rm250-m00334-8-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00334-8-WB-test-result-image-1.pngAnti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 Western Blotting result<br>Western Blot of Jurkat cell lysate, untreated or treated with etoposide, using Anti-Caspase-3 RM250 at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00334-8-IHC-test-result-image-2.pngAnti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Caspase-3 RM250 at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-akt-ser473-rabbit-monoclonal-antibody-clone-rm251-p00024-6-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00024-6-WB-test-result-image-1.pngAnti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251 Western Blotting result<br>Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00024-6-IHC-test-result-image-2.pngAnti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251 at a 1:400 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt1-rabbit-monoclonal-antibody-clone-rm252-m00024-3-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-3-WB-test-result-image-1.pngAnti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252 Western Blotting result<br>Western Blot of 293 cell lysate, using Anti-Akt1 RM252 at a 1:1000 dilution, showed Akt1 (~56 kDa) expression in 293 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-3-IHC-test-result-image-2.pngAnti-Akt1 Rabbit Monoclonal Antibody, Clone#RM252 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Akt1 RM252 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acta2-rabbit-monoclonal-antibody-clone-rm253-m01072-4-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01072-4-WB-test-result-image-1.pngAnti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 Western Blotting result<br>Western Blot of mouse heart tissue lysate, using Anti-alpha smooth muscle Actin RM253 at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01072-4-IHC-test-result-image-2.pngAnti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human heart tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01072-4-IHC-test-result-image-3.pngAnti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ar-n-terminal-rabbit-monoclonal-antibody-clone-rm254-m00542-4-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00542-4-WB-test-result-image-1.pngAnti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 Western Blotting result<br>Western Blot analysis of 22RV1 whole cell lysates, using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254), showed full length androgen receptor and splice variants expressed in 22RV1 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00542-4-IHC-test-result-image-2.pngAnti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254) at a 1:2500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00542-4-IHC-test-result-image-3.pngAnti-AR (N-terminal) Rabbit Monoclonal Antibody, Clone#RM254 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cells sections using anti-Androgen Receptor (N-term) rabbit monoclonal antibody (Clone RM254) at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-paxillin-rabbit-monoclonal-antibody-clone-rm256-m01033-1-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01033-1-WB-test-result-image-1.pngAnti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256 Western Blotting result<br>Western Blot of HeLa cell lysate, using anti-Paxillin rabbit monoclonal Antibody Clone RM256 at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01033-1-ICC-test-result-image-2.pngAnti-Paxillin PXN Rabbit Monoclonal Antibody, Clone#RM256 ICC result<br>Immunocytochemical staining of HeLa cells, using anti-Paxillin rabbit monoclonal Antibody Clone RM256 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-cardiac-actin-actc1-rabbit-monoclonal-antibody-clone-rm257-m03744-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03744-WB-test-result-image-1.pngAnti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 Western Blotting result<br>Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03744-WB-test-result-image-2.pngAnti-Alpha Cardiac Actin (ACTC1) Rabbit Monoclonal Antibody, Clone#RM257 Western Blotting result<br>Western Blot of mouse heart tissue lysate, usingAnti-alpha cardiac actin RM257 at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synaptophysin-rabbit-monoclonal-antibody-clone-rm258-m05049-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05049-2-WB-test-result-image-1.pngAnti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258 Western Blotting result<br>Western Blot analysis of HeLa whole cell lysates, using anti-Synaptophysin rabbit monoclonal antibody (Clone RM258), showed Synaptophysin (~38 kDa) expression in HeLa cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05049-2-IHC-test-result-image-2.pngAnti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue sections using anti-Synaptophysin rabbit monoclonal antibody (Clone RM258) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-n-cadherin-cdh2-rabbit-monoclonal-antibody-clone-rm259-m01577-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01577-2-WB-test-result-image-1.pngAnti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody, Clone#RM259 Western Blotting result<br>Western Blot analysis of HeLa whole cell lysates, using anti-N-cadherin rabbit monoclonal antibody (Clone RM259), showed N-cadherin expression in HeLa cells.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-stat3-tyr705-rabbit-monoclonal-antibody-clone-rm261-p00007-3-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00007-3-WB-test-result-image-1.pngAnti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261 Western Blotting result<br>Western Blot of A431 cell lysates, nontreated (-) or treated (+) with EGF, using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:1000 dilution, showed Phospho-Stat3 (Tyr705) in EGF-treated A431 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00007-3-IHC-test-result-image-2.pngAnti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody, Clone#RM261 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Stat3 (Tyr705) Rabbit Monoclonal Antibody (clone RM261) at a 1:10,000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calponin-1-rabbit-monoclonal-antibody-clone-rm262-m08065-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08065-2-WB-test-result-image-1.pngAnti-Calponin-1 CNN1 Rabbit Monoclonal Antibody, Clone#RM262 Western Blotting result<br>Western Blot analysis of mouse heart tissue lysates, using anti-Calponin-1 rabbit monoclonal antibody (Clone RM262), shows Calponin-1 expression in mouse heart tissue.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08065-2-ICC-test-result-image-2.pngAnti-Calponin-1 CNN1 Rabbit Monoclonal Antibody, Clone#RM262 ICC result<br>Immunocytochemistry of HeLa cells, using Calponin-1 Rabbit Monoclonal Antibody (Clone RM262) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p16ink4a-cdkn2a-rabbit-monoclonal-antibody-clone-rm267-m00016-1-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00016-1-WB-test-result-image-1.pngAnti-p16INK4a (CDKN2A) Rabbit Monoclonal Antibody, Clone#RM267 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-p16INK4a Rabbit Monoclonal Antibody (Clone RM267) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00016-1-IHC-test-result-image-2.pngAnti-p16INK4a (CDKN2A) Rabbit Monoclonal Antibody, Clone#RM267 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Colon cancer tissue sections using Anti-p16INK4a Rabbit Monoclonal Antibody (Clone RM267) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd44-rabbit-monoclonal-antibody-clone-rm264-m00052-2-boster.html2026-03-24T05:24:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00052-2-WB-test-result-image-1.pngAnti-CD44 Rabbit Monoclonal Antibody, Clone#RM264 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-CD44 Rabbit Monoclonal Antibody (Clone RM264) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00052-2-IHC-test-result-image-2.pngAnti-CD44 Rabbit Monoclonal Antibody, Clone#RM264 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD44 Rabbit Monoclonal Antibody (Clone RM264) at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pten-rabbit-monoclonal-antibody-clone-rm265-m00006-3-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00006-3-WB-test-result-image-1.pngAnti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 Western Blotting result<br>Western Blot of HeLa and 293 cells lysates using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00006-3-IHC-test-result-image-2.pngAnti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck8-cytokeratin-8-rabbit-monoclonal-antibody-clone-rm266-m01421-4-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01421-4-WB-test-result-image-1.pngAnti-CK8 (Cytokeratin-8) KRT8 Rabbit Monoclonal Antibody, Clone#RM266 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-CK-8 Rabbit Monoclonal Antibody (Clone RM266) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01421-4-IHC-test-result-image-2.pngAnti-CK8 (Cytokeratin-8) KRT8 Rabbit Monoclonal Antibody, Clone#RM266 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK-8 Rabbit Monoclonal Antibody (Clone RM266) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-4-itga4-rabbit-monoclonal-antibody-clone-rm268-m00468-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00468-1-WB-test-result-image-1.pngAnti-Integrin alpha 4 (ITGA4) Rabbit Monoclonal Antibody, Clone#RM268 Western Blotting result<br>Western Blot of Jurkat cells lysates using Anti-Integrin alpha 4 Rabbit Monoclonal Antibody (Clone RM268) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00468-1-IHC-test-result-image-2.pngAnti-Integrin alpha 4 (ITGA4) Rabbit Monoclonal Antibody, Clone#RM268 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue sections using Anti-Integrin alpha 4 Rabbit Monoclonal Antibody (Clone RM268) at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-egfr-y1173-rabbit-monoclonal-antibody-clone-rm269-p00023-5-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00023-5-WB-test-result-image-1.pngAnti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 Western Blotting result<br>Western Blot of A431 cell nontreated (-) or treated (+) with EGF, using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:500 dilution, showed Phospho-EGFR (Tyr1173) in EGF-treated A431 cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P00023-5-IHC-test-result-image-2.pngAnti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody, Clone#RM269 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-EGFR (Tyr1173) Rabbit Monoclonal Antibody (clone RM269) at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-acetyl-coa-carboxylase-s79-acc1-rabbit-monoclonal-antibody-clone-rm270-p01802-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P01802-WB-test-result-image-1.pngAnti-Phospho Acetyl CoA Carboxylase (S79) (ACC1) ACACA Rabbit Monoclonal Antibody, Clone#RM270 Western Blotting result<br>Western Blot of A431 cell lysates, nontreated (-) or treated (+) with Lambda Protein Phosphatase (PP), using Anti-Phospho-Acetyl CoA Carboxylase (Ser79) Rabbit Monoclonal Antibody (Clone RM269) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P01802-IHC-test-result-image-2.pngAnti-Phospho Acetyl CoA Carboxylase (S79) (ACC1) ACACA Rabbit Monoclonal Antibody, Clone#RM270 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Phospho-Acetyl CoA Carboxylase (Ser79) Rabbit Monoclonal Antibody (clone RM270) at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smac-diablo-rabbit-monoclonal-antibody-clone-rm271-m03790-2-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03790-2-WB-test-result-image-1.pngAnti-Smac/Diablo Rabbit Monoclonal Antibody, Clone#RM271 Western Blotting result<br>Western Blot of HeLa and MCF-7 cells lysates using Anti-Smac/Diablo Rabbit Monoclonal Antibody (Clone RM271) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03790-2-IHC-test-result-image-2.pngAnti-Smac/Diablo Rabbit Monoclonal Antibody, Clone#RM271 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smac/Diablo Rabbit Monoclonal Antibody (Clone RM271) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd20-rabbit-monoclonal-antibody-clone-rm272-m03780-2-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03780-2-WB-test-result-image-1.pngAnti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272 Western Blotting result<br>Western Blot of Raji cell lysates using Anti-CD20 Rabbit Monoclonal Antibody (Clone RM272) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03780-2-IHC-test-result-image-2.pngAnti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using Anti-CD20 Rabbit Monoclonal Antibody (Clone RM272) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p65-rabbit-monoclonal-antibody-clone-rm273-m00284-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00284-1-WB-test-result-image-1.pngAnti-NFkB p65 RELA Rabbit Monoclonal Antibody, Clone#RM273Western Blot of MCF-7 cells lysates using Anti-NFkB p65
Rabbit Monoclonal Antibody (Clone RM273) at a 1:500
dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00284-1-IHC-test-result-image-2.pngAnti-NFkB p65 RELA Rabbit Monoclonal Antibody, Clone#RM273Immunohistochemical staining of formalin fixed and
paraffin embedded human breast cancer tissue
sections using Anti-NFkB p65 Rabbit Monoclonal
Antibody (Clone RM273) at a 1:1250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mtor-rabbit-monoclonal-antibody-clone-rm274-m00003-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00003-1-WB-test-result-image-1.pngAnti-mTOR Rabbit Monoclonal Antibody, Clone#RM274 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-mTOR Rabbit Monoclonal Antibody (Clone RM274) at a 1:1500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00003-1-IHC-test-result-image-2.pngAnti-mTOR Rabbit Monoclonal Antibody, Clone#RM274 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-mTOR Rabbit Monoclonal Antibody (Clone RM274) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-jam1-rabbit-monoclonal-antibody-clone-rm275-m02068-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02068-1-WB-test-result-image-1.pngAnti-JAM1 Rabbit Monoclonal Antibody, Clone#RM275 Western Blotting result<br>Western Blot of HEK293 cells lysates using Anti-JAM1 Rabbit Monoclonal Antibody (Clone RM275) at a 1:250 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02068-1-IHC-test-result-image-2.pngAnti-JAM1 Rabbit Monoclonal Antibody, Clone#RM275 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using Anti-JAM1 Rabbit Monoclonal Antibody (Clone RM275) at a 1:10000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-catenin-1-ctnnb1-rabbit-monoclonal-antibody-clone-rm276-m00004-3-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00004-3-WB-test-result-image-1.pngAnti-Beta-Catenin 1 (CTNNB1) Rabbit Monoclonal Antibody, Clone#RM276 Western Blotting result<br>Western Blot of HeLa and 293 cells lysates using Anti--Catenin Rabbit Monoclonal Antibody (Clone RM276) at a 1:400 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00004-3-IHC-test-result-image-2.pngAnti-Beta-Catenin 1 (CTNNB1) Rabbit Monoclonal Antibody, Clone#RM276 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti--Catenin Rabbit Monoclonal Antibody (Clone RM276) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad4-rabbit-monoclonal-antibody-clone-rm277-m00074-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00074-1-WB-test-result-image-1.pngAnti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 Western Blotting result<br>Western Blot of Jurkat and IMR32 cells lysates using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00074-1-IHC-test-result-image-2.pngAnti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aurora-b-aurkb-rabbit-monoclonal-antibody-clone-rm278-m00762-4-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00762-4-WB-test-result-image-1.pngAnti-Aurora-B (AURKB) Rabbit Monoclonal Antibody, Clone#RM278 Western Blotting result<br>Western Blot of HeLa cells nontreated (-) or treated (+) with Nocodazole (NOC), using Anti-Aurora B Rabbit Monoclonal Antibody (Clone RM278) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00762-4-IHC-test-result-image-2.pngAnti-Aurora-B (AURKB) Rabbit Monoclonal Antibody, Clone#RM278 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using Anti-Aurora B Rabbit Monoclonal Antibody (Clone RM278) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck18-cytokeratin-18-rabbit-monoclonal-antibody-clone-rm279-m01357-3-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01357-3-WB-test-result-image-1.pngAnti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-CK18 Rabbit Monoclonal Antibody (Clone RM279) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01357-3-IHC-test-result-image-2.pngAnti-CK18 (Cytokeratin-18) KRT18 Rabbit Monoclonal Antibody, Clone#RM279 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK18 Rabbit Monoclonal Antibody (Clone RM279) at a 1:4000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neurofilament-l-nf-l-rabbit-monoclonal-antibody-clone-rm280-m02482-5-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02482-5-WB-test-result-image-1.pngAnti-Neurofilament-L (NF-L) NEFL Rabbit Monoclonal Antibody, Clone#RM280 Western Blotting result<br>Western Blot of human brain tissue lysates using Anti-Neurofilament-L (NF-L) Rabbit Monoclonal Antibody (Clone RM280) at a 1:2500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02482-5-IHC-test-result-image-2.pngAnti-Neurofilament-L (NF-L) NEFL Rabbit Monoclonal Antibody, Clone#RM280 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-Neurofilament-L (NF-L) Rabbit Monoclonal Antibody (Clone RM280) at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-b1-rabbit-monoclonal-antibody-clone-rm281-m00745-4-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00745-4-WB-test-result-image-1.pngAnti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 Western Blotting result<br>Western Blot of Jurkat cell lysates using Anti-Cyclin B1 Rabbit Monoclonal Antibody (clone RM281) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00745-4-IHC-test-result-image-2.pngAnti-Cyclin B1 Rabbit Monoclonal Antibody, Clone#RM281 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Cyclin B1 Rabbit Monoclonal Antibody (clone RM281) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck20-cytokeratin-20-rabbit-monoclonal-antibody-clone-rm283-m02828-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02828-1-WB-test-result-image-1.pngAnti-CK20 (Cytokeratin-20) KRT20 Rabbit Monoclonal Antibody, Clone#RM283 Western Blotting result<br>Western Blot of A431 cells lysates using Anti-CK20 Rabbit Monoclonal Antibody (Clone RM283) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02828-1-IHC-test-result-image-2.pngAnti-CK20 (Cytokeratin-20) KRT20 Rabbit Monoclonal Antibody, Clone#RM283 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue sections using Anti-CK20 Rabbit Monoclonal Antibody (Clone RM283) at a 1:250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck7-cytokeratin-7-rabbit-monoclonal-antibody-clone-rm284-m02416-3-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02416-3-WB-test-result-image-1.pngAnti-CK7 (Cytokeratin-7) KRT7 Rabbit Monoclonal Antibody, Clone#RM284 Western Blotting result<br>Western Blot of HeLa cells lysates using Anti-CK7 Rabbit Monoclonal Antibody (Clone RM284) at a 1:2500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02416-3-IHC-test-result-image-2.pngAnti-CK7 (Cytokeratin-7) KRT7 Rabbit Monoclonal Antibody, Clone#RM284 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CK7 Rabbit Monoclonal Antibody (Clone RM284) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd45-rabbit-monoclonal-antibody-clone-rm291-m00555-2-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00555-2-WB-test-result-image-1.pngAnti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291 Western Blotting result<br>Western Blot of Jurkat cell lysates using anti-CD45 rabbit monoclonal antibody (clone RM291) at a 1:2,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00555-2-IHC-test-result-image-2.pngAnti-CD45 PTPRC Rabbit Monoclonal Antibody, Clone#RM291 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using anti-CD45 rabbit monoclonal antibody (clone RM291) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd29-rabbit-monoclonal-antibody-clone-rm285-m00772-3-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00772-3-WB-test-result-image-1.pngAnti-CD29 ITGB1 Rabbit Monoclonal Antibody, Clone#RM285 Western Blotting result<br>Western Blot of HeLa cell lysates using anti-CD29 rabbit monoclonal antibody (clone RM285) at a 1:4000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00772-3-IHC-test-result-image-2.pngAnti-CD29 ITGB1 Rabbit Monoclonal Antibody, Clone#RM285 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using anti-CD29 rabbit monoclonal antibody (clone RM285) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00772-3-IHC-test-result-image-3.pngAnti-CD29 ITGB1 Rabbit Monoclonal Antibody, Clone#RM285 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue sections using anti-CD29 rabbit monoclonal antibody (clone RM285) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-actinin-4-rabbit-monoclonal-antibody-clone-rm287-m01975-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01975-1-WB-test-result-image-1.pngAnti-Alpha Actinin-4 ACTN4 Rabbit Monoclonal Antibody, Clone#RM287 Western Blotting result<br>Western Blot of HeLa cells lysates using anti-alpha-Actinin-4 rabbit monoclonal antibody (clone RM287) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01975-1-IHC-test-result-image-2.pngAnti-Alpha Actinin-4 ACTN4 Rabbit Monoclonal Antibody, Clone#RM287 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-alpha-Actinin-4 rabbit monoclonal antibody (clone RM287) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psd-95-rabbit-monoclonal-antibody-clone-rm288-m02128-1-boster.html2026-03-24T05:24:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02128-1-WB-test-result-image-1.pngAnti-PSD-95 DLG4 Rabbit Monoclonal Antibody, Clone#RM288 Western Blotting result<br>Western Blot of mouse brain lysates using anti-PSD-95 rabbit monoclonal antibody (clone RM288) at a 1:8,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02128-1-IHC-test-result-image-2.pngAnti-PSD-95 DLG4 Rabbit Monoclonal Antibody, Clone#RM288 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using anti-PSD-95 rabbit monoclonal antibody (clone RM288) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vimentin-rabbit-monoclonal-antibody-clone-rm289-m00235-6-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00235-6-WB-test-result-image-1.pngAnti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289 Western Blotting result<br>Western Blot of HeLa lysates using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:400 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00235-6-IHC-test-result-image-2.pngAnti-Vimentin Rabbit Monoclonal Antibody, Clone#RM289 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd11b-integrin-alpha-m-rabbit-monoclonal-antibody-clone-rm290-m00144-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00144-2-WB-test-result-image-1.pngAnti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290 Western Blotting result<br>Western Blot of HL60 cell lysates, nontreated (-) or treated (+) with DMSO using anti-CD11b rabbit monoclonal antibody (clone RM290) at a 1:400 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00144-2-IHC-test-result-image-2.pngAnti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using anti-CD11b rabbit monoclonal antibody (clone RM290) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-er-alpha-rabbit-monoclonal-antibody-clone-rm292-m00057-3-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00057-3-WB-test-result-image-1.pngAnti-ER alpha ESR1 Rabbit Monoclonal Antibody, Clone#RM292 Western Blotting result<br>Western Blot of MCF-7 cell lysates using anti-ER-alpha rabbit monoclonal antibody (clone RM292) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00057-3-IHC-test-result-image-2.pngAnti-ER alpha ESR1 Rabbit Monoclonal Antibody, Clone#RM292 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-ER-alpha rabbit monoclonal antibody (clone RM292) at a 1:100 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lc3b-rabbit-monoclonal-antibody-clone-rm293-m01524-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01524-1-WB-test-result-image-1.pngAnti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 Western Blotting result<br>Western Blot of cell lysates of HeLa untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal antibody (clone RM293) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01524-1-IHC-test-result-image-2.pngAnti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using anti-LC3B rabbit monoclonal antibody (clone RM293) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01524-1-ICC-test-result-image-3.pngAnti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Clone#RM293 ICC result<br>Immunocytochemical staining of HeLa cells untreated or treated with chloroquine (CHQ), using anti-LC3B rabbit monoclonal Antibody Clone RM293 (red) at a 1:200 dilution. Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egf-receptor-rabbit-monoclonal-antibody-clone-rm294-m00023-3-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00023-3-WB-test-result-image-1.pngAnti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294 Western Blotting result<br>Western Blot of HeLa cell lysates using anti-EGF Receptor rabbit monoclonal antibody (clone RM294) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00023-3-IHC-test-result-image-2.pngAnti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-EGF Receptor rabbit monoclonal antibody (clone RM294) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-phospho-ampa-receptor-glur-1-ser845-rabbit-monoclonal-antibody-clone-rm296-p02677-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P02677-2-WB-test-result-image-1.pngAnti-Phospho-AMPA Receptor (GluR 1) (Ser845) GRIA1 Rabbit Monoclonal Antibody, Clone#RM296 Western Blotting result<br>Western Blot of mouse brain tissue lysate, non-treated (-) or dephosphorylated (+) with Lambda Protein Phosphatase (PP), using Anti-Phospho-AMPA Receptor (GluR 1) (Ser845) rabbit monoclonal antibody (clone RM296) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/0/P02677-2-IHC-test-result-image-2.pngAnti-Phospho-AMPA Receptor (GluR 1) (Ser845) GRIA1 Rabbit Monoclonal Antibody, Clone#RM296 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using Anti-Phospho-AMPA Receptor (GluR 1) (Ser845) rabbit monoclonal antibody (clone RM296) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd79a-c-term-rabbit-monoclonal-antibody-clone-rm297-m01047-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01047-2-WB-test-result-image-1.pngAnti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297 Western Blotting result<br>Western Blot of Raji cells lysate using anti-CD79a rabbit monoclonal antibody (Clone RM297) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01047-2-IHC-test-result-image-2.pngAnti-CD79a (C-term) Rabbit Monoclonal Antibody, Clone#RM297 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD79a rabbit monoclonal antibody (Clone RM297) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif-2-alpha-ps51-rabbit-monoclonal-antibody-clone-rm298-m04387-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04387-WB-test-result-image-1.pngAnti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 Western Blotting result<br>Western Blot of HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04387-IHC-test-result-image-2.pngAnti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04387-IHC-test-result-image-3.pngAnti-eIF-2 alpha (pS51) EIF2S1 Rabbit Monoclonal Antibody, Clone#RM298 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-phospho-eIF-2 (Ser51) rabbit monoclonal antibody (clone RM298) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-kappa-b-p105-p50-nfkb-rabbit-monoclonal-antibody-clone-rm299-m00283-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00283-1-WB-test-result-image-1.pngAnti-NF-kappa-B p105/p50 (NFkB) NFKB1 Rabbit Monoclonal Antibody, Clone#RM299 Western Blotting result<br>Western Blot of HeLa cells lysate using anti-NF-kappa-B p105/p50 rabbit monoclonal antibody (clone RM299) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00283-1-IHC-test-result-image-2.pngAnti-NF-kappa-B p105/p50 (NFkB) NFKB1 Rabbit Monoclonal Antibody, Clone#RM299 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-NF-kappa-B p105/p50 rabbit monoclonal antibody (clone RM299) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd34-c-term-rabbit-monoclonal-antibody-clone-rm300-m00885-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00885-2-WB-test-result-image-1.pngAnti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 Western Blotting result<br>Western Blot of mouse spleen tissue lysate using anti-CD34 rabbit monoclonal antibody (Clone RM300) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00885-2-IHC-test-result-image-2.pngAnti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-CD34 rabbit monoclonal antibody (Clone RM300) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ampk-alpha-1-ampka1-5-amp-activated-protein-kinase-catalytic-subunit-alpha-1-rabbit-monoclonal-antibody-clone-rm301-m00994-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00994-1-WB-test-result-image-1.pngAnti-AMPK alpha-1 (AMPKA1) (5'-AMP-activated protein kinase catalytic subunit alpha-1) PRKAA1 Rabbit Monoclonal Antibody, Clone#RM301 Western Blotting result<br>Western Blot of HeLa cells lysate using anti-AMPK alpha-1 rabbit monoclonal antibody (clone RM301) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00994-1-IHC-test-result-image-2.pngAnti-AMPK alpha-1 (AMPKA1) (5'-AMP-activated protein kinase catalytic subunit alpha-1) PRKAA1 Rabbit Monoclonal Antibody, Clone#RM301 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using anti-AMPK alpha-1 rabbit monoclonal antibody (clone RM301) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p27kip1-cyclin-dependent-kinase-inhibitor-1b-rabbit-monoclonal-antibody-clone-rm302-m00173-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00173-2-WB-test-result-image-1.pngAnti-p27Kip1 (Cyclin-dependent kinase inhibitor 1B) CDKN1B Rabbit Monoclonal Antibody, Clone#RM302 Western Blotting result<br>Western Blot of MCF-7 cells lysate using anti-p27Kip1 rabbit monoclonal antibody (clone RM3.2) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00173-2-IHC-test-result-image-2.pngAnti-p27Kip1 (Cyclin-dependent kinase inhibitor 1B) CDKN1B Rabbit Monoclonal Antibody, Clone#RM302 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-p27Kip1 rabbit monoclonal antibody (clone RM302) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd140b-c-term-pdgfr-beta-platelet-derived-growth-factor-receptor-beta-rabbit-monoclonal-antibody-clone-rm303-m00096-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00096-2-WB-test-result-image-1.pngAnti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303 Western Blotting result<br>Western Blot of human brain tissue lysate using anti-CD140b rabbit monoclonal antibody (Clone RM303) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00096-2-IHC-test-result-image-2.pngAnti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-CD140b rabbit monoclonal antibody (Clone RM303) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100-beta-rabbit-monoclonal-antibody-clone-rm304-m00979-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00979-2-WB-test-result-image-1.pngAnti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 Western Blotting result<br>Western Blot of human brain tissue lysate using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00979-2-IHC-test-result-image-2.pngAnti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00979-2-IHC-test-result-image-3.pngAnti-S100-beta Rabbit Monoclonal Antibody, Clone#RM304 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-S100B rabbit monoclonal antibody (Clone RM304) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myd88-rabbit-monoclonal-antibody-clone-rm306-m00025-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00025-1-WB-test-result-image-1.pngAnti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306 Western Blotting result<br>Western Blot of K562 cells lysate using anti-MyD88 rabbit monoclonal antibody (Clone RM306) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00025-1-IHC-test-result-image-2.pngAnti-MYD88 Rabbit Monoclonal Antibody, Clone#RM306 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-MyD88 rabbit monoclonal antibody (Clone RM306) at a 1:250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-braf-rabbit-monoclonal-antibody-clone-rm308-m00075-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00075-2-WB-test-result-image-1.pngAnti-BRAF Rabbit Monoclonal Antibody, Clone#RM308 Western Blotting result<br>Western Blot of K562 cells lysate using anti-B-raf rabbit monoclonal antibody (Clone RM308) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00075-2-IHC-test-result-image-2.pngAnti-BRAF Rabbit Monoclonal Antibody, Clone#RM308 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-B-raf rabbit monoclonal antibody (Clone RM308) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pd-1-cd279-rabbit-monoclonal-antibody-clone-rm309-m00178-2-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00178-2-WB-test-result-image-1.pngAnti-PD-1 (CD279) Rabbit Monoclonal Antibody, Clone#RM309 Western Blotting result<br>Western Blot the lysates of Jurkat cells non-treated (-) or treated (+) with PHA and PMA, using anti-PD-1 (CD279) rabbit monoclonal antibody (Clone RM309) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00178-2-IHC-test-result-image-2.pngAnti-PD-1 (CD279) Rabbit Monoclonal Antibody, Clone#RM309 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-PD-1 rabbit monoclonal antibody (Clone RM309) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag-1l-rabbit-monoclonal-antibody-clone-rm310-m02423-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-WB-test-result-image-1.pngAnti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 Western Blotting result<br>Western Blot of HeLa cell lysate, using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-IHC-test-result-image-2.pngAnti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-IHC-test-result-image-3.pngAnti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded 22RV1 cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-IHC-test-result-image-4.pngAnti-BAG-1L Rabbit Monoclonal Antibody, Clone#RM310 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded BAG-1L overexpressing LNCaP cell section using anti-BAG-1L rabbit monoclonal antibody (Clone RM310) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neun-fox3-rbfox3-rabbit-monoclonal-antibody-clone-rm312-m11954-4-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11954-4-WB-test-result-image-1.pngAnti-NeuN (Fox3, RBFOX3) Rabbit Monoclonal Antibody, Clone#RM312 Western Blotting result<br>Western Blot of human brain tissue lysate using anti-NeuN rabbit monoclonal antibody (Clone RM312) at a 1:1,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/1/M11954-4-IHC-test-result-image-2.pngAnti-NeuN (Fox3, RBFOX3) Rabbit Monoclonal Antibody, Clone#RM312 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-NeuN rabbit monoclonal antibody (Clone RM312) at a 1:1,000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ox40-cd134-rabbit-monoclonal-antibody-clone-rm313-m02495-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02495-1-WB-test-result-image-1.pngAnti-OX40 (CD134) Rabbit Monoclonal Antibody, Clone#RM313 Western Blotting result<br>Western Blot of human spleen tissue lysate using anti-OX40 (CD134) rabbit monoclonal antibody (Clone RM313) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02495-1-IHC-test-result-image-2.pngAnti-OX40 (CD134) Rabbit Monoclonal Antibody, Clone#RM313 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-OX40 (CD134) rabbit monoclonal antibody (Clone RM313) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd5-rabbit-monoclonal-antibody-clone-rm314-m02591-1-boster.html2026-03-24T05:24:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02591-1-WB-test-result-image-1.pngAnti-CD5 Rabbit Monoclonal Antibody, Clone#RM314 Western Blotting result<br>Western Blot of Jurkat cells lysate using anti-CD5 rabbit monoclonal antibody (Clone RM314) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02591-1-IHC-test-result-image-2.pngAnti-CD5 Rabbit Monoclonal Antibody, Clone#RM314 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD5 rabbit monoclonal antibody (Clone RM314) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd56-rabbit-monoclonal-antibody-clone-rm315-m00184-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00184-2-WB-test-result-image-1.pngAnti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315 Western Blotting result<br>Western Blot of human brain tissue lysate using anti-CD56 rabbit monoclonal antibody (Clone RM315) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00184-2-IHC-test-result-image-2.pngAnti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-CD56 rabbit monoclonal antibody (Clone RM315) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt-ph-domain-rabbit-monoclonal-antibody-clone-rm316-m00024-4-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-4-WB-test-result-image-1.pngAnti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316 Western Blotting result<br>Western Blot of 293 cells lysate using anti-AKT (PH domain) rabbit monoclonal antibody (Clone RM316) at a 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-4-IHC-test-result-image-2.pngAnti-AKT (PH domain) AKT1 Rabbit Monoclonal Antibody, Clone#RM316 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-AKT (PH domain) rabbit monoclonal antibody (Clone RM316) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plap-rabbit-monoclonal-antibody-clone-rm317-m01718-1-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01718-1-WB-test-result-image-1.pngAnti-PLAP Rabbit Monoclonal Antibody, Clone#RM317 Western Blotting result<br>Western Blot of A431 cells lysate using anti-PLAP rabbit monoclonal antibody (Clone RM317) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01718-1-IHC-test-result-image-2.pngAnti-PLAP Rabbit Monoclonal Antibody, Clone#RM317 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human placenta tissue section using anti-PLAP rabbit monoclonal antibody (Clone RM317) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-acetyl-alpha-tubulin-lys40-rabbit-monoclonal-antibody-clone-rm318-m08382-3-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08382-3-WB-test-result-image-1.pngAnti-Acetyl-alpha-Tubulin (Lys40) TUBA1B Rabbit Monoclonal Antibody, Clone#RM318 Western Blotting result<br>Western Blot analysis of HeLa cell lysates non-treated (-) or treated (+) with Trichostatin A (TSA), using anti-Acetyl-alpha-Tubulin (Lys40) rabbit monoclonal antibody (Clone RM318) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M08382-3-ICC-test-result-image-2.pngAnti-Acetyl-alpha-Tubulin (Lys40) TUBA1B Rabbit Monoclonal Antibody, Clone#RM318 ICC result<br>Immunocytochemistry of HeLa cells non-treated or treated with Trichostatin A (TSA), using anti-Acetyl-alpha-Tubulin (Lys40) rabbit monoclonal antibody (Clone RM318) at a 1:5000 dilution (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei with DAPI (blue).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pd-l1-cd274-b7-h1-rabbit-monoclonal-antibody-clone-rm320-m00109-1-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00109-1-WB-test-result-image-1.pngAnti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320Western Blot of lysates H1975, DU145, and
LNCaP cells, using anti-PD-L1 rabbit monoclonal
antibody (Clone RM320) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00109-1-ihc-test-result-image-2.pngAnti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320Immunohistochemical staining of formalin fixed
and paraffin embedded human lung cancer tissue
section using anti-PD-L1 rabbit monoclonal antibody
(Clone RM320) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00109-2-ihc-test-result-image-3.pngAnti-PD-L1 (CD274) (B7-H1) Rabbit Monoclonal Antibody, Clone#RM320Immunohistochemical staining of formalin fixed
and paraffin embedded human tonsil tissue section
using anti-PD-L1 rabbit monoclonal antibody (Clone
RM320) at a 1:250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psa-prostate-specific-antigen-rabbit-monoclonal-antibody-clone-rm323-m01505-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01505-2-WB-test-result-image-1.pngAnti-PSA (Prostate Specific Antigen) Rabbit Monoclonal Antibody, Clone#RM323 Western Blotting result<br>Western Blot of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non-reduced (-) or reduced (+) conditions, using anti-PSA rabbit monoclonal antibody (Clone RM323) at a 1:2,500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01505-2-IHC-test-result-image-2.pngAnti-PSA (Prostate Specific Antigen) Rabbit Monoclonal Antibody, Clone#RM323 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using anti-PSA rabbit monoclonal antibody (Clone RM323) at a 1:1,000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calretinin-calb2-rabbit-monoclonal-antibody-clone-rm324-m04255-5-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04255-5-WB-test-result-image-1.pngAnti-Calretinin (CALB2) Rabbit Monoclonal Antibody, Clone#RM324 Western Blotting result<br>Western Blot of A431 cell lysate using anti-Calretinin rabbit monoclonal antibody (Clone RM324) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04255-5-IHC-test-result-image-2.pngAnti-Calretinin (CALB2) Rabbit Monoclonal Antibody, Clone#RM324 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-Calretinin rabbit monoclonal antibody (Clone RM324) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-1-rabbit-monoclonal-antibody-clone-rm325-m00179-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00179-2-WB-test-result-image-1.pngAnti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:40,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00179-2-IHC-test-result-image-2.pngAnti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human liver tissue section using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cea-cd66e-rabbit-monoclonal-antibody-clone-rm326-m00356-3-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00356-3-WB-test-result-image-1.pngAnti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326 Western Blotting result<br>Western Blot of MCF-7 cell lysate using anti-CEA rabbit monoclonal antibody (Clone RM326) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00356-3-IHC-test-result-image-2.pngAnti-CEA (CD66e) Rabbit Monoclonal Antibody, Clone#RM326 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using anti-CEA rabbit monoclonal antibody (Clone RM326) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psma-rabbit-monoclonal-antibody-clone-rm327-m02846-1-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02846-1-WB-test-result-image-1.pngAnti-PSMA FOLH1 Rabbit Monoclonal Antibody, Clone#RM327 Western Blotting result<br>Western Blot of LNCaP cell lysate using anti-PSMA rabbit monoclonal antibody (Clone RM327) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02846-1-IHC-test-result-image-2.pngAnti-PSMA FOLH1 Rabbit Monoclonal Antibody, Clone#RM327 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human prostate cancer tissue section using anti-PSMA rabbit monoclonal antibody (Clone RM327) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck-14-cytokeratin-14-rabbit-monoclonal-antibody-clone-rm328-m01432-3-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01432-3-WB-test-result-image-1.pngAnti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328 Western Blotting result<br>Western Blot of A431 cell lysate using anti-CK-14 rabbit monoclonal antibody (Clone RM328) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01432-3-IHC-test-result-image-2.pngAnti-CK-14 (Cytokeratin-14) KRT14 Rabbit Monoclonal Antibody, Clone#RM328 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-14 rabbit monoclonal antibody (Clone RM328) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hcg-human-chorionic-gonadotropin-rabbit-monoclonal-antibody-clone-rm330-m00739-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00739-2-WB-test-result-image-1.pngAnti-hCG (Human Chorionic Gonadotropin) CGA Rabbit Monoclonal Antibody, Clone#RM330 Western Blotting result<br>Western Blot of hCG protein purified from human urine using anti-hCG rabbit monoclonal antibody (Clone RM330) at a 1:20,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00739-2-IHC-test-result-image-2.pngAnti-hCG (Human Chorionic Gonadotropin) CGA Rabbit Monoclonal Antibody, Clone#RM330 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human placenta tissue section using anti-hCG rabbit monoclonal antibody (Clone RM330) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pax-5-paired-box-protein-rabbit-monoclonal-antibody-clone-rm331-m00669-1-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-1-WB-test-result-image-1.pngAnti-PAX-5 (paired box protein) Rabbit Monoclonal Antibody, Clone#RM331 Western Blotting result<br>Western Blot of Raji cell lysate using anti-Paired box protein Pax-5 rabbit monoclonal antibody (Clone RM331) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-1-IHC-test-result-image-2.pngAnti-PAX-5 (paired box protein) Rabbit Monoclonal Antibody, Clone#RM331 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-Paired box protein Pax-5 rabbit monoclonal antibody (Clone RM331) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd19-rabbit-monoclonal-antibody-clone-rm332-m00154-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00154-2-WB-test-result-image-1.pngAnti-CD19 Rabbit Monoclonal Antibody, Clone#RM332 Western Blotting result<br>Western Blot of Raji cell lysate using anti-CD19 rabbit monoclonal antibody (Clone RM332) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00154-2-IHC-test-result-image-2.pngAnti-CD19 Rabbit Monoclonal Antibody, Clone#RM332 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-CD19 rabbit monoclonal antibody (Clone RM332) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mart1-rabbit-monoclonal-antibody-clone-rm333-m02033-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02033-2-WB-test-result-image-1.pngAnti-MART1 MLANA Rabbit Monoclonal Antibody, Clone#RM333 Western Blotting result<br>Western Blot of SK-MEL-2 cell lysate using anti-MART1 rabbit monoclonal antibody (Clone RM333) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02033-2-IHC-test-result-image-2.pngAnti-MART1 MLANA Rabbit Monoclonal Antibody, Clone#RM333 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human melanoma tissue section using anti-MART1 rabbit monoclonal antibody (Clone RM333) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-surfactant-protein-a-sp-a-rabbit-monoclonal-antibody-clone-rm334-m04967-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04967-WB-test-result-image-1.pngAnti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334 Western Blotting result<br>Western Blot of human lung tissue lysate using anti-Surfactant protein A (SP-A) rabbit monoclonal antibody (Clone RM334) at a 1:10,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04967-IHC-test-result-image-2.pngAnti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-Surfactant protein A (SP-A) rabbit monoclonal antibody (Clone RM334) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt-e17k-mutant-rabbit-monoclonal-antibody-clone-rm336-m00024-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-2-WB-test-result-image-1.pngAnti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 Western Blotting result<br>Western Blot analysis of a cell lysate mixture of untransfected 293T and 293T transfected with a DNA construct encoding the Akt E17K mutant, using anti-Akt E17K (clone RM336) (0.1ug/mL) or anti-Akt (clone RM252) rabbit monoclonal antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00024-2-IHC-test-result-image-2.pngAnti-Akt (E17K Mutant) AKT1 Rabbit Monoclonal Antibody, Clone#RM336 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding the Akt E17K mutation or Histone H3.3, stained with anti-Akt E17K rabbit monoclonal antibody (clone RM336) at 0.2ug/mL.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd276-b7-h3-rabbit-monoclonal-antibody-clone-rm335-m02220-1-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02220-1-WB-test-result-image-1.pngAnti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 Western Blotting result<br>Western Blot of LNCaP cell lysate using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:10,000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02220-1-IHC-test-result-image-2.pngAnti-CD276 (B7-H3) Rabbit Monoclonal Antibody, Clone#RM335 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-CD276 (B7-H3) rabbit monoclonal antibody (Clone RM335) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd10-rabbit-monoclonal-antibody-clone-rm337-m04065-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04065-2-WB-test-result-image-1.pngAnti-CD10 MME Rabbit Monoclonal Antibody, Clone#RM337 Western Blotting result<br>Western Blot of Raji cell lysate using anti-CD10 rabbit monoclonal antibody (Clone RM337) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04065-2-IHC-test-result-image-2.pngAnti-CD10 MME Rabbit Monoclonal Antibody, Clone#RM337 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Kidney tissue section using anti-CD10 rabbit monoclonal antibody (Clone RM337) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04065-2-IHC-test-result-image-3.pngAnti-CD10 MME Rabbit Monoclonal Antibody, Clone#RM337 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-CD10 rabbit monoclonal antibody (Clone RM337) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-crotonyl-histone-h3-lys9-rabbit-monoclonal-antibody-clone-rm339-m06819-27-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-27-WB-test-result-image-1.pngAnti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339 Western Blotting result<br>Western Blot using Anti-Histone H3K9cr Rabbit Monoclonal Antibody RM339 against H3K9cr [Crotonyl-Histone H3 (Lys9)]. Anti-Histone H3 and anti-G6PDH were used as controls. A crotonylation inducing metabolite was used to increase the H3K9cr signal.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-27-Dot-Blot-test-result-image-2.pngAnti-Crotonyl-Histone H3 (Lys9) H3F3A Rabbit Monoclonal Antibody, Clone#RM339 Dot Blot result<br>A Peptide dotblot showing Anti-Histone H3K9cr Rabbit Monoclonal Antibody RM339 reacts specifically to Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl), and RM339
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-trimethyl-histone-h3-lys4-rabbit-monoclonal-antibody-clone-rm340-m06819-2-boster.html2026-03-24T05:24:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-2-ELISA-test-result-image-1.pngAnti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340 ELISA result showing specificity<br>ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified), using anti-H3K4me3 rabbit monoclonal antibody clone RM340 (10 g/mL). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M06819-2-ChIP-test-result-image-2.pngAnti-Trimethyl-Histone H3 (Lys4) H3F3A Rabbit Monoclonal Antibody, Clone#RM340 ChIP result<br>SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit monoclonal antibody clone RM340. Antibody (3 g at 17 g/mL) was tested in native ChIP with 3 g HEK-293 chromatin (~1x106 cells). Specificity (left Y-axis
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ck-5-ck-6-cytokeratin-5-cytokeratin-6-rabbit-monoclonal-antibody-clone-rm341-m00398-4-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00398-4-WB-test-result-image-1.pngAnti-CK-5/CK-6 (Cytokeratin 5/ Cytokeratin 6) Rabbit Monoclonal Antibody, Clone#RM341 Western Blotting result<br>Western Blot of A431 cell lysates using RM341 using anti-CK-5/CK-6 rabbit monoclonal antibody (Clone RM341) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00398-4-IHC-test-result-image-2.pngAnti-CK-5/CK-6 (Cytokeratin 5/ Cytokeratin 6) Rabbit Monoclonal Antibody, Clone#RM341 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-5/CK-6 rabbit monoclonal antibody (Clone RM341) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd3e-rabbit-monoclonal-antibody-clone-rm344-m02675-3-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02675-3-WB-test-result-image-1.pngAnti-CD3E Rabbit Monoclonal Antibody, Clone#RM344 Western Blotting result<br>Western Blot of Jurkat cell lysate using anti-CD3e rabbit monoclonal antibody (Clone RM344) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02675-3-IHC-test-result-image-2.pngAnti-CD3E Rabbit Monoclonal Antibody, Clone#RM344 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-CD3e rabbit monoclonal antibody (Clone RM344) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd4-rabbit-monoclonal-antibody-clone-rm345-m00344-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00344-1-WB-test-result-image-1.pngAnti-CD4 Rabbit Monoclonal Antibody, Clone#RM345 Western Blotting result<br>Western Blot of Jurkat cell lysate using anti-CD4 rabbit monoclonal antibody (Clone RM345) at a 1:2500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00344-1-IHC-test-result-image-2.pngAnti-CD4 Rabbit Monoclonal Antibody, Clone#RM345 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-CD4 rabbit monoclonal antibody (Clone RM345) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-spastin-rabbit-monoclonal-antibody-clone-rm346-m00521-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00521-WB-test-result-image-1.pngAnti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 Western Blotting result<br>Western Blot of lysate from HeLa cells, transfected with Control siRNA (1) or Spastin siRNA, using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00521-IHC-test-result-image-2.pngAnti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Brain tissue section using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00521-IHC-test-result-image-3.pngAnti-Spastin Rabbit Monoclonal Antibody, Clone#RM346 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Tonsil tissue section using anti-Spastin rabbit monoclonal antibody (Clone RM346) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gst3-gst-pi-rabbit-monoclonal-antibody-clone-rm347-m00394-2-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00394-2-WB-test-result-image-1.pngAnti-GST3/GST pi GSTP1 Rabbit Monoclonal Antibody, Clone#RM347 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-GSTP1 rabbit monoclonal antibody (Clone RM347) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00394-2-IHC-test-result-image-2.pngAnti-GST3/GST pi GSTP1 Rabbit Monoclonal Antibody, Clone#RM347 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Brain tissue section using anti-GSTP1 rabbit monoclonal antibody (Clone RM347) at a 1:20000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00394-2-IHC-test-result-image-3.pngAnti-GST3/GST pi GSTP1 Rabbit Monoclonal Antibody, Clone#RM347 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Liver tissue section using anti-GSTP1 rabbit monoclonal antibody (Clone RM347) at a 1:20000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cox-2-rabbit-monoclonal-antibody-clone-rm348-m00084-2-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00084-2-WB-test-result-image-1.pngAnti-COX-2 PTGS2 Rabbit Monoclonal Antibody, Clone#RM348 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-COX-2 rabbit monoclonal antibody (Clone RM348) at a 1:20000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00084-2-IHC-test-result-image-2.pngAnti-COX-2 PTGS2 Rabbit Monoclonal Antibody, Clone#RM348 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-COX-2 rabbit monoclonal antibody (Clone RM348) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p504s-amacr-rabbit-monoclonal-antibody-clone-rm349-m02217-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02217-1-WB-test-result-image-1.pngAnti-p504s/AMACR Rabbit Monoclonal Antibody, Clone#RM349 Western Blotting result<br>Western Blot of LNCaP cell lysate using anti-p504s/AMACR rabbit monoclonal antibody (Clone RM349) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02217-1-IHC-test-result-image-2.pngAnti-p504s/AMACR Rabbit Monoclonal Antibody, Clone#RM349 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-p504s/AMACR rabbit monoclonal antibody (Clone RM349) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stathmin-stmn1-rabbit-monoclonal-antibody-clone-rm350-m01194-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01194-1-WB-test-result-image-1.pngAnti-Stathmin (STMN1) Rabbit Monoclonal Antibody, Clone#RM350 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-Stathmin rabbit monoclonal antibody (Clone RM350) at a 1:5000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01194-1-IHC-test-result-image-2.pngAnti-Stathmin (STMN1) Rabbit Monoclonal Antibody, Clone#RM350 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-Stathmin rabbit monoclonal antibody (Clone RM350) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-17-ck-17-rabbit-monoclonal-antibody-clone-rm351-m02101-3-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-3-WB-test-result-image-1.pngAnti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-CK-17 rabbit monoclonal antibody (Clone RM351) at a 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-3-IHC-test-result-image-2.pngAnti-Cytokeratin 17 (CK-17) KRT19 Rabbit Monoclonal Antibody, Clone#RM351 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-17 rabbit monoclonal antibody (Clone RM351) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mum1-irf4-rabbit-monoclonal-antibody-clone-rm352-m00401-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00401-1-WB-test-result-image-1.pngAnti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 Western Blotting result<br>Western Blot of Raji cell lysate using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00401-1-IHC-test-result-image-2.pngAnti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:250 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00401-1-IHC-test-result-image-3.pngAnti-MUM1/IRF4 Rabbit Monoclonal Antibody, Clone#RM352 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human melanoma tissue section using anti-MUM1/IRF4 rabbit monoclonal antibody (Clone RM352) at a 1:250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p120-catenin-rabbit-monoclonal-antibody-clone-rm353-m02333-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02333-1-WB-test-result-image-1.pngAnti-p120-catenin Rabbit Monoclonal Antibody, Clone#RM353 Western Blotting result<br>Western Blot of A431 cell lysate using anti-p120 catenin rabbit monoclonal antibody (Clone RM353) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02333-1-IHC-test-result-image-2.pngAnti-p120-catenin Rabbit Monoclonal Antibody, Clone#RM353 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-p120 catenin rabbit monoclonal antibody (Clone RM353) at a 1:5000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd5-rabbit-monoclonal-antibody-clone-rm354-m02591-2-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02591-2-WB-test-result-image-1.pngAnti-CD5 Rabbit Monoclonal Antibody, Clone#RM354 Western Blotting result<br>Western Blot of Jurkat cells lysate using anti-CD5 rabbit monoclonal antibody (Clone RM354) at a 1:25000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02591-2-IHC-test-result-image-2.pngAnti-CD5 Rabbit Monoclonal Antibody, Clone#RM354 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD5 rabbit monoclonal antibody (Clone RM354) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-4-ck-4-rabbit-monoclonal-antibody-clone-rm355-m07410-2-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07410-2-WB-test-result-image-1.pngAnti-Cytokeratin 4 (CK-4) Rabbit Monoclonal Antibody, Clone#RM355 Western Blotting result<br>Western Blot of A431 cell lysate using anti-CK-4 rabbit monoclonal antibody (Clone RM355) at a 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M07410-2-IHC-test-result-image-2.pngAnti-Cytokeratin 4 (CK-4) Rabbit Monoclonal Antibody, Clone#RM355 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-4 rabbit monoclonal antibody (Clone RM355) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag-1-rabbit-monoclonal-antibody-clone-rm356-m02423-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-1-WB-test-result-image-1.pngAnti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356 Western Blotting result<br>Western Blot of HeLa cell lysate using anti-BAG-1 rabbit monoclonal antibody (Clone RM356) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02423-1-IHC-test-result-image-2.pngAnti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-BAG-1 rabbit monoclonal antibody (Clone RM356) at a 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pr-progesterone-receptor-rabbit-monoclonal-antibody-clone-rm357-m00541-3-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00541-3-WB-test-result-image-1.pngAnti-PR (Progesterone Receptor) PGR Rabbit Monoclonal Antibody, Clone#RM357 Western Blotting result<br>Western Blot of T47D cell lysate using anti-Progesterone Receptor (PR) rabbit monoclonal antibody (clone RM357) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00541-3-IHC-test-result-image-2.pngAnti-PR (Progesterone Receptor) PGR Rabbit Monoclonal Antibody, Clone#RM357 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-Progesterone Receptor (PR) rabbit monoclonal antibody (clone RM357) at a 1:100 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd117-c-kit-rabbit-monoclonal-antibody-clone-rm359-m01335-2-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01335-2-WB-test-result-image-1.pngAnti-CD117/c-Kit Rabbit Monoclonal Antibody, Clone#RM359 Western Blotting result<br>Western Blot of human placenta tissue lysate using anti-CD117/c-Kit rabbit monoclonal antibody (Clone RM359) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01335-2-IHC-test-result-image-2.pngAnti-CD117/c-Kit Rabbit Monoclonal Antibody, Clone#RM359 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-CD117/c-Kit rabbit monoclonal antibody (Clone RM359) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01335-2-IHC-test-result-image-3.pngAnti-CD117/c-Kit Rabbit Monoclonal Antibody, Clone#RM359 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD117/c-Kit rabbit monoclonal antibody (Clone RM359) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ki67-rabbit-monoclonal-antibody-clone-rm360-m00254-5-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00254-5-IHC-test-result-image-1.pngAnti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections, using anti-Ki-67 Rabbit Monoclonal Antibody (Clone RM360) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alk-rabbit-monoclonal-antibody-clone-rm361-m00301-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00301-1-WB-test-result-image-1.pngAnti-ALK Rabbit Monoclonal Antibody, Clone#RM361 Western Blotting result<br>Western Blot of NSCLC cell line H2228 (Cell Lysate) expressing EML4-ALK variant 3, using anti-ALK rabbit monoclonal antibody (Clone RM361) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00301-1-IHC-test-result-image-2.pngAnti-ALK Rabbit Monoclonal Antibody, Clone#RM361 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded NCI-H2228 cells (expressing EML4-ALK variant 3) and Daudi cells (ALK negative), stained with anti-ALK rabbit monoclonal antibody clone RM361.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00301-1-IHC-test-result-image-3.pngAnti-ALK Rabbit Monoclonal Antibody, Clone#RM361 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-ALK rabbit monoclonal antibody (Clone RM361) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-19-ck-19-rabbit-monoclonal-antibody-clone-rm364-m02101-4-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-4-WB-test-result-image-1.pngAnti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364 Western Blotting result<br>Western Blot of MCF-7 cell lysate using anti-CK-19 rabbit monoclonal antibody (Clone RM364) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02101-4-IHC-test-result-image-2.pngAnti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-CK-19 rabbit monoclonal antibody (Clone RM364) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-satb2-rabbit-monoclonal-antibody-clone-rm365-m02588-1-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02588-1-WB-test-result-image-1.pngAnti-SATB2 Rabbit Monoclonal Antibody, Clone#RM365 Western Blotting result<br>Western Blot of K562 cell lysate using anti-SATB2 rabbit monoclonal antibody (Clone RM365) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02588-1-IHC-test-result-image-2.pngAnti-SATB2 Rabbit Monoclonal Antibody, Clone#RM365 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-SATB2 rabbit monoclonal antibody (Clone RM365) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-napsin-a-rabbit-monoclonal-antibody-clone-rm366-m05685-3-boster.html2026-03-24T05:24:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05685-3-WB-test-result-image-1.pngAnti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366 Western Blotting result<br>Western Blot of human lung tissue lysate using anti-Napsin-A rabbit monoclonal antibody (Clone RM366) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05685-3-WB-test-result-image-2.pngAnti-Napsin-A NAPSA Rabbit Monoclonal Antibody, Clone#RM366 Western Blotting result<br>Western Blot of human lung tissue lysate using anti-Napsin-A rabbit monoclonal antibody (Clone RM366) at a 1:2000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syntaxin-1a-rabbit-monoclonal-antibody-clone-rm367-m01961-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01961-1-WB-test-result-image-1.pngAnti-Syntaxin-1A Rabbit Monoclonal Antibody, Clone#RM367 Western Blotting result<br>Western Blot of mouse brain tissue lysate using anti-Syntaxin-1A rabbit monoclonal antibody (Clone RM367) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01961-1-IHC-test-result-image-2.pngAnti-Syntaxin-1A Rabbit Monoclonal Antibody, Clone#RM367 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-Syntaxin-1A rabbit monoclonal antibody (Clone RM367) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tpo-thyroid-peroxidase-rabbit-monoclonal-antibody-clone-rm368-m00320-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00320-1-WB-test-result-image-1.pngAnti-TPO (Thyroid Peroxidase) Rabbit Monoclonal Antibody, Clone#RM368 Western Blotting result<br>Western Blot of TT cell lysate using anti-TPO rabbit monoclonal antibody (Clone RM368) at a 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00320-1-IHC-test-result-image-2.pngAnti-TPO (Thyroid Peroxidase) Rabbit Monoclonal Antibody, Clone#RM368 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid tissue section using anti-TPO rabbit monoclonal antibody (Clone RM368) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myod1-rabbit-monoclonal-antibody-clone-rm369-m00964-2-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00964-2-WB-test-result-image-1.pngAnti-MyoD1 Rabbit Monoclonal Antibody, Clone#RM369 Western Blotting result<br>Western Blot of mouse skeletal muscle tissue lysate using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00964-2-IHC-test-result-image-2.pngAnti-MyoD1 Rabbit Monoclonal Antibody, Clone#RM369 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human Rhabdomyosarcoma section using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp-b-surfactant-protein-b-rabbit-monoclonal-antibody-clone-rm370-m03441-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03441-WB-test-result-image-1.pngAnti-SP-B (Surfactant protein B) Rabbit Monoclonal Antibody, Clone#RM370 Western Blotting result<br>Western Blot of human lung tissue lysate using anti-SP-B rabbit monoclonal antibody (Clone RM370) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03441-IHC-test-result-image-2.pngAnti-SP-B (Surfactant protein B) Rabbit Monoclonal Antibody, Clone#RM370 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-SP-B rabbit monoclonal antibody (Clone RM370) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd163-rabbit-monoclonal-antibody-clone-rm371-m00812-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00812-1-WB-test-result-image-1.pngAnti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 Western Blotting result<br>Western Blot of human placenta tissue lysate using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00812-1-IHC-test-result-image-2.pngAnti-CD163 Rabbit Monoclonal Antibody, Clone#RM371 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human spleen tissue section using anti-CD163 rabbit monoclonal antibody (Clone RM371) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd21-rabbit-monoclonal-antibody-clone-rm372-m01632-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01632-1-WB-test-result-image-1.pngAnti-CD21 CR2 Rabbit Monoclonal Antibody, Clone#RM372 Western Blotting result<br>Western Blot of Daudi lysate using anti-CD21 rabbit monoclonal antibody (Clone RM372) at a 1:400 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01632-1-IHC-test-result-image-2.pngAnti-CD21 CR2 Rabbit Monoclonal Antibody, Clone#RM372 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CD21 rabbit monoclonal antibody (Clone RM372) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ttf1-thyroid-transcription-factor-1-rabbit-monoclonal-antibody-clone-rm373-m01322-2-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01322-2-WB-test-result-image-1.pngAnti-TTF1 (Thyroid transcription factor 1) NKX2-1 Rabbit Monoclonal Antibody, Clone#RM373 Western Blotting result<br>Western Blot of TT cell lysate using anti-TTF1 rabbit monoclonal antibody (Clone RM373) at a 1:20000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01322-2-IHC-test-result-image-2.pngAnti-TTF1 (Thyroid transcription factor 1) NKX2-1 Rabbit Monoclonal Antibody, Clone#RM373 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-TTF1 rabbit monoclonal antibody (Clone RM373) at a 1:1000 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-fos-rabbit-monoclonal-antibody-clone-rm374-m00297-5-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00297-5-WB-test-result-image-1.pngAnti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 Western Blotting result<br>Western Blot of HeLa cell lysates: non-treated (-) or treated (+) with TPA, using anti-TTF1 rabbit monoclonal antibody (Clone RM374) at a 1:5000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00297-5-IHC-test-result-image-2.pngAnti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid cancer tissue section using anti-c-Fos rabbit monoclonal antibody (Clone RM374) at a 1:1250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-msh2-rabbit-monoclonal-antibody-clone-rm375-m00140-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00140-1-WB-test-result-image-1.pngAnti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375 Western Blotting result<br>Western Blot of HeLa lysate using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00140-1-IHC-test-result-image-2.pngAnti-MSH2 Rabbit Monoclonal Antibody, Clone#RM375 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH2 rabbit monoclonal antibody (Clone RM375) at a 1:100 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-msh6-rabbit-monoclonal-antibody-clone-rm376-m00553-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00553-1-WB-test-result-image-1.pngAnti-MSH6 Rabbit Monoclonal Antibody, Clone#RM376 Western Blotting result<br>Western Blot of 293 lysate using anti-MSH6 rabbit monoclonal antibody (Clone RM376) at a 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00553-1-IHC-test-result-image-2.pngAnti-MSH6 Rabbit Monoclonal Antibody, Clone#RM376 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue section using anti-MSH6 rabbit monoclonal antibody (Clone RM376) at a 1:100 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arginase-1-arg1-rabbit-monoclonal-antibody-clone-rm377-m01106-3-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01106-3-WB-test-result-image-1.pngAnti-Arginase-1 (ARG1) Rabbit Monoclonal Antibody, Clone#RM377 Western Blotting result<br>Western Blot of human liver tissue lysate using anti-ARG1 rabbit monoclonal antibody (Clone RM377) at a 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01106-3-IHC-test-result-image-2.pngAnti-Arginase-1 (ARG1) Rabbit Monoclonal Antibody, Clone#RM377 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human liver cancer tissue section using anti-ARG1 rabbit monoclonal antibody (Clone RM377) at a 1:1250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bob-1-obf-1-rabbit-monoclonal-antibody-clone-rm378-m04431-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04431-1-WB-test-result-image-1.pngAnti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 Western Blotting result<br>Western Blot of Daudi cells lysate using anti-BOB-1/OBF-1 rabbit monoclonal antibody (Clone RM378) at a 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04431-1-IHC-test-result-image-2.pngAnti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-BOB-1/OBF-1 rabbit monoclonal antibody (Clone RM378) at a 1:1250 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-terminal-deoxynucleotidyl-transferase-tdt-rabbit-monoclonal-antibody-clone-rm379-m05546-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05546-1-WB-test-result-image-1.pngAnti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 Western Blotting result<br>Western Blot of Jurkat cells lysate using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:2000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05546-1-IHC-test-result-image-2.pngAnti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human thymus tissue section using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:200 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-matrix-metalloproteinase-12-mmp-12-rabbit-monoclonal-antibody-clone-rm381-m01520-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01520-1-WB-test-result-image-1.pngAnti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381 Western Blotting result<br>Western Blot of HEK 293 cells lysate using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01520-1-IHC-test-result-image-2.pngAnti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM381 IHC result<br>Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-MMP-12 rabbit monoclonal antibody (Clone RM381) at a 1:2500 dilution.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ig-light-chains-rabbit-monoclonal-antibody-clone-rm129-m05470-4-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-4-ELISA-test-result-image-1.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 ELISA result showing specificity<br>ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-4-ELISA-test-result-image-2.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 ELISA result showing specificity<br>ELISA shows that clone RM129 reacts only to the kappa and lambda light chains in all human immunoglobulins, and does not react to mouse IgG, rat IgG, or goat IgG. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-4-ELISA-test-result-image-3.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 ELISA result showing specificity<br>A titer ELISA using RM129. The plate was coated with different amounts of human IgG4. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05470-4-ELISA-test-result-image-4.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 ELISA result showing specificity<br>A titer ELISA using RM129. The plate was coated with different amounts of human IgG3. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ig-light-chains-rabbit-monoclonal-antibody-biotin-conjugated-clone-rm129-b05470-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/B/0/B05470-ELISA-test-result-image-1.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 ELISA result showing specificity<br>Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/B/0/B05470-ELISA-test-result-image-2.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 ELISA result showing specificity<br>ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/B/0/B05470-ELISA-test-result-image-4.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 ELISA result showing specificity<br>ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/B/0/B05470-ELISA-test-result-image-5.pngAnti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody Biotin Conjugated, Clone#RM129 ELISA result showing specificity<br>A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-igg-goat-monoclonal-antibody-clone-rmg07-m04575-3-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-3-ELISA-test-result-image-1.pngAnti-Mouse IgG goat monoclonal antibody, Clone#RMG07 ELISA result showing specificity<br>A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RMG07 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04575-3-ELISA-test-result-image-2.pngAnti-Mouse IgG goat monoclonal antibody, Clone#RMG07 ELISA result showing specificity<br>A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RMG07 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
https://www.bosterbio.com/products/primary-antibodies/anti-mint3-apba3-picoband-trade-antibody-a07396-2-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07396-2-apba3-primary-antibodies-wb-testing-1.jpgAnti-Mint3/APBA3 Antibody Picoband® Western blot analysis of APBA3 using anti-APBA3 antibody (A07396-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBA3 antigen affinity purified polyclonal antibody (Catalog # A07396-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBA3 at approximately 69 kDa. The expected band size for APBA3 is at 61 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-liver-arginase-arg1-picoband-trade-antibody-a01106-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01106-41598_2024_76563_fig4_html.pngAnti-liver Arginase/ARG1 Antibody Picoband®Effects of co-culture of trophoblasts and macrophages treated with hypoxia on cellular functions. A. THP-1-derived macrophages (lower chamber) were co-cultured with HTR-8/Svneo cells (upper chamber). B. The levels of IL-1α, IL-12, and TNF-α in cell supernatants were detected by ELISA. C. Identification of the macrophage phenotype by flow cytometry. D. Identification of the macrophage phenotype by western blot analysis of phenotype markers: iNOS and CD68 for the M1-phenotype; Arg1 and CD206 for the M2-phenotype. Furthermore, THP-1-derived macrophages were treated with the supernatants of hypoxia-pretreated or non-treated HTR-8/Svneo cells E. The levels of IL-1α, IL-12, and TNF-α in cell supernatants were detected by ELISA. F. Relative expression of miR-141-3p in macrophages. G . Identification of the phenotypes of macrophages by flow cytometry. H. Identification of the phenotypes of macrophages by western blot analysis: iNOS and CD68 for the M1-phenotype; Arg1 and CD206 for the M2-phenotype. HTR-8/Svneo: HTR-8/Svneo cells cultured alone; THP-1: THP-1-derived macrophages cultured alone; HTR-8/Svneo + THP-1: HTR-8/Svneo cells co-cultured with THP-1-derived macrophages; Hyo-HTR-8/Svneo + THP-1: hypoxia-treated HTR-8/Svneo cells co-cultured with THP-1-derived macrophages; HTR-8/Svneo supernatant + THP-1: THP-1-derived macrophages treated with supernatant from HTR-8/Svneo cells; Hypo-HTR-8/Svneo supernatant + THP-1: THP-1-derived macrophages treated with supernatant from hypoxia-treated HTR-8/Svneo cells. ns: no significance; * p < 0.05, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-76563-y'>39424901</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01106-arg1-primary-antibodies-wb-testing-1.jpgAnti-liver Arginase/ARG1 Antibody Picoband® Western blot analysis of ARG1 using anti-ARG1 antibody (A01106). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCCP tissue lysates, <br>
Lane 2: monkey liver tissue lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: mouse liver tissue lysates, <br>
Lane 5: rat testis tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 37 kDa. The expected band size for ARG1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01106-arg1-primary-antibodies-ihc-testing-2.jpgAnti-liver Arginase/ARG1 Antibody Picoband® IHC analysis of ARG1 using anti-ARG1 antibody (A01106). <br>
ARG1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARG1 Antibody (A01106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01106-arg1-primary-antibodies-ihc-testing-3.jpgAnti-liver Arginase/ARG1 Antibody Picoband® IHC analysis of ARG1 using anti-ARG1 antibody (A01106). <br>
ARG1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARG1 Antibody (A01106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01106-arg1-primary-antibodies-ihc-testing-4.jpgAnti-liver Arginase/ARG1 Antibody Picoband® IHC analysis of ARG1 using anti-ARG1 antibody (A01106). <br>
ARG1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARG1 Antibody (A01106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd160-picoband-trade-antibody-a06168-1-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06168-1-CD160-primary-antibodies-WB-testing-1.jpgAnti-CD160 Antibody Picoband® Western blot analysis of CD160 using anti-CD160 antibody (A06168-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD160 antigen affinity purified polyclonal antibody (Catalog # A06168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD160 at approximately 27KD. The expected band size for CD160 is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06168-1-CD160-primary-antibodies-WB-testing-2.jpgAnti-CD160 Antibody Picoband® Western blot analysis of CD160 using anti-CD160 antibody (A06168-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD160 antigen affinity purified polyclonal antibody (Catalog # A06168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD160 at approximately 27KD. The expected band size for CD160 is at 20KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cdc42-picoband-trade-antibody-a00119-boster.html2026-03-24T05:24:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-ihc-testing-1.jpgAnti-CDC42 Antibody Picoband® IHC analysis of CDC42 using anti-CDC42 antibody (A00119). <br>
CDC42 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-ihc-testing-2.jpgAnti-CDC42 Antibody Picoband® IHC analysis of CDC42 using anti-CDC42 antibody (A00119). <br>
CDC42 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-ihc-testing-3.jpgAnti-CDC42 Antibody Picoband® IHC analysis of CDC42 using anti-CDC42 antibody (A00119). <br>
CDC42 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-ihc-testing-4.jpgAnti-CDC42 Antibody Picoband® IHC analysis of CDC42 using anti-CDC42 antibody (A00119). <br>
CDC42 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-wb-testing-5.jpgAnti-CDC42 Antibody Picoband® Western blot analysis of CDC42 using anti-CDC42 antibody (A00119). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human U937 whole cell lysates, <br>
Lane 3: human HL-60 whole cell lysates, <br>
Lane 4: human U20S whole cell lysates, <br>
Lane 5: human U87 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates, <br>
Lane 8: rat thymus tissue lysates, <br>
Lane 9: rat lung tissue lysates, <br>
Lane 10: mouse lung tissue lysates, <br>
Lane 11: mouse intestines tissue lysates, <br>
Lane 12: mouse SP2/0 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (Catalog # A00119) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42 at approximately 21KD. The expected band size for CDC42 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-fcm-testing-6.pngAnti-CDC42 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CDC42 antibody (A00119). <br>Overlay histogram showing SiHa cells stained with A00119 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC42 Antibody (A00119, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-cdc42-primary-antibodies-fcm-testing-7.pngAnti-CDC42 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-CDC42 antibody (A00119). <br>Overlay histogram showing U20S cells stained with A00119 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC42 Antibody (A00119, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cdk5-picoband-trade-antibody-a00511-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00511-1-cdk5-primary-antibodies-wb-testing-1.jpgAnti-CDK5 Antibody Picoband® Western blot analysis of CDK5 using anti-CDK5 antibody (A00511-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 antigen affinity purified polyclonal antibody (Catalog # A00511-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK5 at approximately 33 kDa. The expected band size for CDK5 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00511-1-cdk5-primary-antibodies-ihc-testing-7.jpgAnti-CDK5 Antibody Picoband®IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1). <br>CDK5 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00511-1-CDK5-primary-antibodies-IHC-testing-2.jpgAnti-CDK5 Antibody Picoband® IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1).<br>CDK5 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00511-1-CDK5-primary-antibodies-IHC-testing-3.jpgAnti-CDK5 Antibody Picoband® IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1).<br>CDK5 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00511-1-CDK5-primary-antibodies-IHC-testing-4.jpgAnti-CDK5 Antibody Picoband® IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1).<br>CDK5 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00511-1-CDK5-primary-antibodies-IHC-testing-5.jpgAnti-CDK5 Antibody Picoband® IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1).<br>CDK5 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00511-1-CDK5-primary-antibodies-IHC-testing-6.jpgAnti-CDK5 Antibody Picoband® IHC analysis of CDK5 using anti-CDK5 antibody (A00511-1).<br>CDK5 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK5 Antibody (A00511-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-ch-tog-ckap5-picoband-trade-antibody-a05324-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-fcm-testing-7.jpgAnti-ch TOG/CKAP5 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ch TOG/CKAP5 antibody (A05324). <br>Overlay histogram showing U937 cells stained with A05324 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ch TOG/CKAP5 Antibody (A05324, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-wb-testing-1.jpgAnti-ch TOG/CKAP5 Antibody Picoband® Western blot analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human U87 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ch TOG/CKAP5 antigen affinity purified polyclonal antibody (Catalog # A05324) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ch TOG/CKAP5 at approximately 225 kDa. The expected band size for ch TOG/CKAP5 is at 225 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-ihc-testing-2.jpgAnti-ch TOG/CKAP5 Antibody Picoband® IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
ch TOG/CKAP5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ch TOG/CKAP5 Antibody (A05324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-ihc-testing-3.jpgAnti-ch TOG/CKAP5 Antibody Picoband® IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
ch TOG/CKAP5 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ch TOG/CKAP5 Antibody (A05324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-ihc-testing-4.jpgAnti-ch TOG/CKAP5 Antibody Picoband® IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
ch TOG/CKAP5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ch TOG/CKAP5 Antibody (A05324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-ihc-testing-5.jpgAnti-ch TOG/CKAP5 Antibody Picoband® IHC analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
ch TOG/CKAP5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ch TOG/CKAP5 Antibody (A05324) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05324-ckap5-primary-antibodies-if-testing-6.jpgAnti-ch TOG/CKAP5 Antibody Picoband® IF analysis of ch TOG/CKAP5 using anti-ch TOG/CKAP5 antibody (A05324). <br>
ch TOG/CKAP5 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ch TOG/CKAP5 Antibody (A05324) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-crb1-picoband-trade-antibody-a01499-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-IHC-testing-1.jpgAnti-CRB1 Antibody Picoband® IHC analysis of CRB1 using anti-CRB1 antibody (A01499-1).<br>CRB1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRB1 Antibody (A01499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-IHC-testing-2.jpgAnti-CRB1 Antibody Picoband® IHC analysis of CRB1 using anti-CRB1 antibody (A01499-1).<br>CRB1 was detected in paraffin-embedded section of human testis cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRB1 Antibody (A01499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-IHC-testing-3.jpgAnti-CRB1 Antibody Picoband® IHC analysis of CRB1 using anti-CRB1 antibody (A01499-1).<br>CRB1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRB1 Antibody (A01499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-IHC-testing-4.jpgAnti-CRB1 Antibody Picoband® IHC analysis of CRB1 using anti-CRB1 antibody (A01499-1).<br>CRB1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRB1 Antibody (A01499-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-FC-testing-5.jpgAnti-CRB1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-CRB1 antibody (A01499-1).<br>Overlay histogram showing U87 cells stained with A01499-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRB1 Antibody (A01499-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01499-1-CRB1-primary-antibodies-WB-testing-6.jpgAnti-CRB1 Antibody Picoband® Western blot analysis of CRB1 using anti-CRB1 antibody (A01499-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRB1 antigen affinity purified polyclonal antibody (Catalog # A01499-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRB1 at approximately 154KD. The expected band size for CRB1 is at 154KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-2a6-cyp2a6-picoband-trade-antibody-a00947-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00947-2-CYP2A6-primary-antibodies-WB-testing-1.jpgAnti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband® Western blot analysis of CYP2A6 using anti-CYP2A6 antibody (A00947-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat RH35 whole cell lysates, <br>
Lane 2: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2A6 antigen affinity purified polyclonal antibody (Catalog # A00947-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2A6 at approximately 57KD. The expected band size for CYP2A6 is at 57KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-2c19-cyp2c19-antibody-a02102-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02102-2-CYP2C19-primary-antibodies-IHC-testing-1.jpgAnti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband® IHC analysis of CYP2C19 using anti-CYP2C19 antibody (A02102-2).<br>CYP2C19 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2C19 Antibody (A02102-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02102-2-CYP2C19-primary-antibodies-IHC-testing-2.jpgAnti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband® IHC analysis of CYP2C19 using anti-CYP2C19 antibody (A02102-2).<br>CYP2C19 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2C19 Antibody (A02102-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02102-2-CYP2C19-primary-antibodies-IHC-testing-3.jpgAnti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband® IHC analysis of CYP2C19 using anti-CYP2C19 antibody (A02102-2).<br>CYP2C19 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2C19 Antibody (A02102-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02102-2-CYP2C19-primary-antibodies-WB-testing-4.jpgAnti-Cytochrome p450 2C19/CYP2C19 Antibody Picoband® Western blot analysis of CYP2C19 using anti-CYP2C19 antibody (A02102-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2C19 antigen affinity purified polyclonal antibody (Catalog # A02102-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2C19 at approximately 56KD. The expected band size for CYP2C19 is at 56KD.
https://www.bosterbio.com/products/primary-antibodies/anti-dazap1-picoband-trade-antibody-a07947-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07947-1-dazap1-primary-antibodies-wb-testing-1.jpgAnti-DAZAP1 Antibody Picoband® Western blot analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human AGS whole cell lysates,<br>
Lane 2: human GES-1 whole cell lysates,<br>
Lane 3: human HGC-27 whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: human Jurkat whole cell lysates,<br>
Lane 6: human 293T whole cell lysates,<br>
Lane 7: rat PC-12 whole cell lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZAP1 antigen affinity purified polyclonal antibody (Catalog # A07947-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZAP1 at approximately 43 kDa. The expected band size for DAZAP1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07947-1-dazap1-primary-antibodies-ihc-testing-4.jpgAnti-DAZAP1 Antibody Picoband®IHC analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1). <br>DAZAP1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAZAP1 Antibody (A07947-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07947-1-DAZAP1-primary-antibodies-IHC-testing-2.jpgAnti-DAZAP1 Antibody Picoband® IHC analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1).<br>DAZAP1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZAP1 Antibody (A07947-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07947-1-dazap1-primary-antibodies-ihc-testing-3.jpgAnti-DAZAP1 Antibody Picoband® IHC analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1).<br>DAZAP1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZAP1 Antibody (A07947-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-kat9-elp3-picoband-trade-antibody-a02833-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02833-1-ELP3-primary-antibodies-WB-testing-1.jpgAnti-KAT9/ELP3 Antibody Picoband® Western blot analysis of ELP3 using anti-ELP3 antibody (A02833-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
Lane 6: human U-87MG whole cell lysates, <br>
Lane 7: human Caco-2 whole cell lysates, <br>
Lane 8: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELP3 antigen affinity purified polyclonal antibody (Catalog # A02833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELP3 at approximately 62KD. The expected band size for ELP3 is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02833-1-ELP3-primary-antibodies-WB-testing-2.jpgAnti-KAT9/ELP3 Antibody Picoband® Western blot analysis of ELP3 using anti-ELP3 antibody (A02833-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse kidney tissue lysates, <br>
Lane 2: mouse lung tissue lysates, <br>
Lane 3: mouse NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELP3 antigen affinity purified polyclonal antibody (Catalog # A02833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELP3 at approximately 62KD. The expected band size for ELP3 is at 62KD.
https://www.bosterbio.com/products/primary-antibodies/anti-epo-antibody-a00484-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00484-2-EPO-primary-antibodies-WB-testing-1.jpgAnti-Erythropoietin EPO Antibody Picoband® Western blot analysis of EPO using anti-EPO antibody (A00484-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
Lane 4: mouse kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 29KD. The expected band size for EPO is at 21-34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00484-2-EPO-primary-antibodies-WB-testing-2.jpgAnti-Erythropoietin EPO Antibody Picoband® Western blot analysis of EPO using anti-EPO antibody (A00484-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 29KD. The expected band size for EPO is at 21-34KD.
https://www.bosterbio.com/products/primary-antibodies/anti-kmt6-ezh2-picoband-trade-antibody-a00050-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00050-1-ezh2-primary-antibodies-wb-testing-1.jpgAnti-KMT6/EZH2 Antibody Picoband® Western blot analysis of KMT6/EZH2 using anti-KMT6/EZH2 antibody (A00050-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: mouse spleen tissue lysates,<br>
Lane 7: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT6/EZH2 antigen affinity purified polyclonal antibody (Catalog # A00050-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT6/EZH2 at approximately 98 kDa. The expected band size for KMT6/EZH2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00050-1-ezh2-primary-antibodies-fcm-testing-2.jpgAnti-KMT6/EZH2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-KMT6/EZH2 antibody (A00050-1). <br>
Overlay histogram showing K562 cells stained with A00050-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KMT6/EZH2 Antibody (A00050-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00050-1-sci2022-9993393.005.jpgAnti-KMT6/EZH2 Antibody Picoband®HOTTIP regulates EZH2 to inhibit PTEN expression. Note: (a) catRAPID predicted binding region of HOTTIP and EZH2. (b) FISH analyzed the localization of EZH2 and HOTTIP in IR-K562 cells. (c) RIP-PCR were used to test the interaction between EZH2 and HOTTIP. ∗ p < 0.05 vs. IgG. (d) RT-qPCR and Western blot were used to measure EZH2 mRNA and protein level after knocking down HOTTIP. (e) Western blot analysis was used to measure EZH2 in BM-MNCs of CML patients. Right panel, bar chart of protein densitometric analysis. ∗∗∗ p < 0.001. RT-qPCR was used to detect EZH2 level in BM-MNCs of CML patients. Normalized to GAPDH. ∗∗∗ p < 0.001 vs. NC. Western blot analysis was used to measure EZH2 protein level in IR-K562 and K562 cells. Right panel, bar chart of protein densitometric analysis. ∗∗∗ p < 0.001; RT-qPCR was used to detect EZH2 mRNA level in CML cell lines (K562 and IR-K562). Normalized to GAPDH. ∗∗∗ P < 0.001 vs. NC. (f) RT-qPCR and Western blot were used to detect EZH2 and PTEN mRNA and protein levels after transfecting with specific sh-con or sh-EZH2. ∗∗∗ P < 0.001 vs. DMSO.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9477575/&orig_host=www.ncbi.nlm.nih.gov'>36117724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00050-1-sci2022-9993393.006.jpgAnti-KMT6/EZH2 Antibody Picoband®HOTTIP work through EZH2 to participate in drug resistance. Note: (a) IR-K562 cells were transfected with specific sh-HOTTIP or both sh-HOTTIP+sh-EZH2. The CCK-8 analysis was used to detect cell proliferation. (b) Cell apoptosis was detected by flow cytometry using Annexin V/APC/PI. The right panel shows the apoptosis rate from three independent experiments. (c) IR-K562 cells were prepared with different Imatinib concentrations for 48 h. The CCK-8 analysis was used to detect cell inhibition. (d) WB was used to detect protein levels.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9477575/&orig_host=www.ncbi.nlm.nih.gov'>36117724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00050-1-kmt6-ezh2-primary-antibodies-ip-testing-3.jpgAnti-KMT6/EZH2 Antibody Picoband® Immunoprecipitating KMT6/EZH2 in MCF-7 whole cell lysate.<br>
Western blot analysis of KMT6/EZH2 using anti-KMT6/EZH2 antibody (A00050-1).<br>
Lane 1: MCF-7 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-KMT6/EZH2 antibody in MCF-7 whole cell lysate;<br>
Lane 3: anti-KMT6/EZH2 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KMT6/EZH2 antigen affinity purified polyclonal antibody (A00050-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for KMT6/EZH2 at approximately 98 kDa. The expected band size for KMT6/EZH2 is at 85 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-frzb-picoband-trade-antibody-a03539-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03539-2-FRZB_SFRP3_-primary-antibodies-WB-testing-1.jpgAnti-FRZB Antibody Picoband® Western blot analysis of FRZB(SFRP3) using anti-FRZB(SFRP3) antibody (A03539-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: rat heart tissue lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse kidney tissue lysates, <br>
Lane 6: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRZB(SFRP3) antigen affinity purified polyclonal antibody (Catalog # A03539-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRZB(SFRP3) at approximately 36KD. The expected band size for FRZB(SFRP3) is at 36KD.
https://www.bosterbio.com/products/primary-antibodies/anti-gli2-antibody-a00701-5-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-IHC-testing-1.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® IHC analysis of GLI2 using anti-GLI2 antibody (A00701-5).<br>GLI2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLI2 Antibody (A00701-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-5-12935_2021_1775_fig2_html.pngAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband®ROC1 regulation of tumor cell growth through hedgehog signaling. a , b Western blot. Stable ROC1-overexpressed and transient ROC1 siRNA-transfected 5637 ( a ) and T24 ( b ) cells were grown and subjected to western blot analysis of cyclin D1 and Cdc25c expression. c , d qRT-PCR. Stable ROC1-overexpressed and transient ROC1 siRNA-transfected 5637 and T24 cells were grown and subjected to qRT-PCR analysis of Gli1 and PTCH1. e , f Western blot. Transient ROC1 siRNA-transfected 5637 cells were treated with SAG ( e ), stable ROC1-overexpressed T24 cells were treated with the hedgehog signaling pathway inhibitor GDC0449 ( f ), and then the cells were subjected to western blot analysis of Gli1 and Gli2. Bars, SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-01775-5'>33499884</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-5-12935_2021_1775_fig3_html.pngAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband®ROC1 modulation of SUFU protein levels. a , b Western blot. Expression of SUFU protein in 5637 ( a ) and T24 ( b ) cells after transfection with siROC1 or plasmid-ROC1 for 48, 72, 96, and 120 h. c qPCR. Bladder cancer 5637 cells were cotransfected with siRNA targeting ROC1 and SUFU and then subjected to qRT-PCR analysis of Gli1 and PTCH1 mRNA. d Western blot. Bladder cancer 5637 cells were cotransfected with siRNA targeting ROC1 and SUFU and then subjected to western blot analysis of Gli2 and SUFU protein. e , f Immunoprecipitation. Immunoprecipitation of SUFU from 5637 cells ( e ) and T24 cells ( f ) that were transfected with either siROC1 or pROC1. Nonspecific rabbit immunoglobulin G (IgG) was used as a negative control. Cell lysates were subjected to western blot analysis. Bars, SEM; * P < 0.05, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-01775-5'>33499884</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-5-12935_2021_1775_fig4_html.pngAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband®ROC1 regulation of SUFU ubiquitination for degradation. a , b Western blot. ROC1-knocked down and ROC1-overexpressed 5637 ( a ) and T24 ( b ) cells were treated with cycloheximide (CHX) for the indicated time points and then subjected to western blot analysis of SUFU protein. The graph shows the quantified data of the western blots depicted in the bottom panel. c , d Coimmunoprecipitation. Detection of ubiquitylated SUFU in 5637 ( c ) and T24 ( d ) cells cotransfected with HA-tagged ubiquitin (Ub) along with either siROC1 or pROC1 by western blot analysis of the cell lysates. Immunoprecipitation of HA antibody of nonspecific rabbit immunoglobulin G (IgG) was used as a negative control. e , f Western blot. 5637 ( e ) and T24 ( f ) cells were treated with the CRL inhibitor MLN4924 at different concentrations and then subjected to western blot analysis of Gli2, cyclin D1, Cdc25c, and SUFU proteins <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-01775-5'>33499884</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00701-5-12935_2021_1775_fig5_html.pngAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband®ROC1 expression in human bladder cancer tissues. a The expression of ROC1, SUFU, and Gli2 proteins was immunohistochemically analyzed in human bladder cancer tissues. Representative immunohistochemical images of low-grade or high-grade cancer are shown. b Association of ROC1, SUFU, and Gli2 expression with the clinicopathological grade. The expression levels were divided into two categories (low vs. high) according to their immunoreactivity scores and were associated with the cancer pathological grade (low-grade vs. high-grade) by using the χ 2 test. Scale bar, 50 µm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-01775-5'>33499884</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-IHC-testing-2.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® IHC analysis of GLI2 using anti-GLI2 antibody (A00701-5).<br>GLI2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLI2 Antibody (A00701-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-IHC-testing-3.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® IHC analysis of GLI2 using anti-GLI2 antibody (A00701-5).<br>GLI2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLI2 Antibody (A00701-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-IHC-testing-4.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® IHC analysis of GLI2 using anti-GLI2 antibody (A00701-5).<br>GLI2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLI2 Antibody (A00701-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-WB-testing-5.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® Western blot analysis of GLI2 using anti-GLI2 antibody (A00701-5). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (Catalog # A00701-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI2 at approximately 210KD. The expected band size for GLI2 is at 168KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00701-5-GLI2-primary-antibodies-WB-testing-6.jpgAnti-Zinc finger protein GLI2 GLI2 Antibody Picoband® Western blot analysis of GLI2 using anti-GLI2 antibody (A00701-5). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: mouse thymus tissue lysates, <br>
Lane 3: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (Catalog # A00701-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI2 at approximately 210KD. The expected band size for GLI2 is at 168KD.
https://www.bosterbio.com/products/primary-antibodies/anti-glycophorin-a-gypa-picoband-trade-antibody-a02184-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02184-2-GYPA-primary-antibodies-IHC-testing-1.jpgAnti-Glycophorin A/GYPA Antibody Picoband® IHC analysis of GYPA using anti-GYPA antibody (A02184-2).<br>GYPA was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GYPA Antibody (A02184-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02184-2-GYPA-primary-antibodies-IHC-testing-2.jpgAnti-Glycophorin A/GYPA Antibody Picoband® IHC analysis of GYPA using anti-GYPA antibody (A02184-2).GYPA was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GYPA Antibody (A02184-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02184-2-GYPA-primary-antibodies-IHC-testing-3.jpgAnti-Glycophorin A/GYPA Antibody Picoband® IHC analysis of GYPA using anti-GYPA antibody (A02184-2).GYPA was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GYPA Antibody (A02184-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02184-2-GYPA-primary-antibodies-WB-testing-4.jpgAnti-Glycophorin A/GYPA Antibody Picoband® Western blot analysis of GYPA using anti-GYPA antibody (A02184-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human MDA-MB-453 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYPA antigen affinity purified polyclonal antibody (Catalog # A02184-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GYPA at approximately 38KD. The expected band size for GYPA is at 16KD.
https://www.bosterbio.com/products/primary-antibodies/anti-histone-h2a-x-h2afx-picoband-trade-antibody-a00241-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-1-h2afx-primary-antibodies-wb-testing-1.jpgAnti-Histone H2A.X/H2AFX Antibody Picoband® Western blot analysis of H2AFX using anti-H2AFX antibody (A00241-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates,<br>
Lane 4: rat PC-12 whole cell lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified polyclonal antibody (Catalog # A00241-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-1-h2afx-primary-antibodies-ihc-testing-2.jpgAnti-Histone H2A.X/H2AFX Antibody Picoband® IHC analysis of H2AFX using anti-H2AFX antibody (A00241-1). <br>
H2AFX was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-H2AFX Antibody (A00241-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-1-h2afx-primary-antibodies-ihc-testing-3.jpgAnti-Histone H2A.X/H2AFX Antibody Picoband® IHC analysis of H2AFX using anti-H2AFX antibody (A00241-1). <br>
H2AFX was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-H2AFX Antibody (A00241-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-1-h2afx-primary-antibodies-ip-testing-1.jpgAnti-Histone H2A.X/H2AFX Antibody Picoband®Immunoprecipitating (IP) H2AFX in 293T whole cell lysate.<br>
Western blot analysis of H2AFX using anti-H2AFX antibody (A00241-1); <br>
Lane 1: 293T whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-H2AFX antibody in 293T whole cell lysate;<br>
Lane 3: anti-H2AFX antibody (2μg) + 293T whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-H2AFX antigen affinity purified polyclonal antibody (A00241-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-1-h2afx-primary-antibodies-fcm-testing-4.jpgAnti-Histone H2A.X/H2AFX Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-H2AFX antibody (A00241-1). <br>
Overlay histogram showing PC-3 cells stained with A00241-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-H2AFX Antibody (A00241-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hdac3-picoband-trade-antibody-a00839-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00839-hdac3-primary-antibodies-wb-testing-1_1.jpgAnti-HDAC3 Antibody Picoband® Western blot analysis of HDAC3 using anti-HDAC3 antibody (A00839). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human MOLT-4 whole cell lysates, <br>
Lane 5: human MCF-7 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates, <br>
Lane 8: rat thymus tissue lysates, <br>
Lane 9: rat C6 whole cell lysates, <br>
Lane 10: mouse ANA-1 whole cell lysates, <br>
Lane 11: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (Catalog # A00839) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00839-hdac3-primary-antibodies-fcm-testing-2.jpgAnti-HDAC3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-HDAC3 antibody (A00839). <br>Overlay histogram showing MCF-7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00839-hdac3-primary-antibodies-fcm-testing-3.jpgAnti-HDAC3 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-HDAC3 antibody (A00839). <br>Overlay histogram showing RAW264.7 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00839-hdac3-primary-antibodies-fcm-testing-4.jpgAnti-HDAC3 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-HDAC3 antibody (A00839). <br>Overlay histogram showing RH35 cells stained with A00839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (A00839, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-il-31-picoband-trade-antibody-a08339-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08339-1-IL31-primary-antibodies-IHC-testing-1.jpgAnti-IL-31 Antibody Picoband® IHC analysis of IL31 using anti-IL31 antibody (A08339-1).<br>IL31 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08339-1-IL31-primary-antibodies-IHC-testing-2.jpgAnti-IL-31 Antibody Picoband® IHC analysis of IL31 using anti-IL31 antibody (A08339-1).<br>IL31 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08339-1-IL31-primary-antibodies-IHC-testing-3.jpgAnti-IL-31 Antibody Picoband® IHC analysis of IL31 using anti-IL31 antibody (A08339-1).<br>IL31 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL31 Antibody (A08339-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08339-1-IL31-primary-antibodies-WB-testing-4.jpgAnti-IL-31 Antibody Picoband® Western blot analysis of IL31 using anti-IL31 antibody (A08339-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL31 antigen affinity purified polyclonal antibody (Catalog # A08339-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL31 at approximately 29KD. The expected band size for IL31 is at 18KD.
https://www.bosterbio.com/products/primary-antibodies/anti-integrin-alpha-6-itga6-picoband-trade-antibody-a01693-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01693-itga6-primary-antibodies-wb-testing-1.jpgAnti-Integrin alpha 6/ITGA6 Antibody Picoband® Western blot analysis of ITGA6 using anti-ITGA6 antibody (A01693). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: rat kidney tissue lysates,<br>
Lane 4: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 antigen affinity purified polyclonal antibody (Catalog # A01693) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA6 at approximately 127 kDa. The expected band size for ITGA6 is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01693-itga6-primary-antibodies-fcm-testing-2.jpgAnti-Integrin alpha 6/ITGA6 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ITGA6 antibody (A01693). <br>
Overlay histogram showing HepG2 cells stained with A01693 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGA6 Antibody (A01693, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-jak3-picoband-trade-antibody-a02598-3-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02598-3-JAK3-primary-antibodies-WB-testing-1.jpgAnti-JAK3 Antibody Picoband® Western blot analysis of JAK3 using anti-JAK3 antibody (A02598-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human PC-3 whole cell lysates<br>Lane 2: human A549 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAK3 antigen affinity purified polyclonal antibody (Catalog # A02598-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAK3 at approximately 125KD. The expected band size for JAK3 is at 125KD.
https://www.bosterbio.com/products/primary-antibodies/anti-kcnh2-antibody-a00781-1-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00781-1-KCNH2-primary-antibodies-WB-testing-1.jpgAnti-KCNH2 Antibody Picoband® Western blot analysis of KCNH2 using anti-KCNH2 antibody (A00781-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysates<br>Lane 2: human U2OS whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH2 at approximately 150KD. The expected band size for KCNH2 is at 127KD.
https://www.bosterbio.com/products/primary-antibodies/anti-lamin-b-receptor-lbr-picoband-trade-antibody-a01238-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01238-2-LBR-primary-antibodies-WB-testing-1_2.jpgAnti-Lamin B Receptor/LBR Antibody Picoband® Western blot analysis of LBR using anti-LBR antibody (A01238-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates<br>Lane 2: human HL-60 whole cell lysates<br>Lane 3: human K562 whole cell lysates<br>Lane 4: human THP-1 whole cell lysates<br>Lane 5: human HEK293 whole cell lysates<br>Lane 6: human U2OS whole cell lysates<br>Lane 7: human U-937 whole cell lysates<br>Lane 8: human Caco-2 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (Catalog # A01238-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBR at approximately 65-71KD. The expected band size for LBR is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01238-2-LBR-primary-antibodies-WB-testing-2_2.jpgAnti-Lamin B Receptor/LBR Antibody Picoband® Western blot analysis of LBR using anti-LBR antibody (A01238-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat thymus tissue lysates<br>Lane 2: rat spleen tissue lysates<br>Lane 3: rat testicular tissue lysates<br>Lane 4: mouse thymus tissue lysates<br>Lane 5: mouse stomach tissue lysates<br>Lane 6: mouse testicular tissue lysates<br>Lane 7: mouse SP20 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBR antigen affinity purified polyclonal antibody (Catalog # A01238-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBR at approximately 65-71KD. The expected band size for LBR is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01238-2-LBR-primary-antibodies-IHC-testing-3_2.jpgAnti-Lamin B Receptor/LBR Antibody Picoband® IHC analysis of LBR using anti-LBR antibody (A01238-2).<br>LBR was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LBR Antibody (A01238-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01238-2-lbr-primary-antibodies-if-testing-4.jpgAnti-Lamin B Receptor/LBR Antibody Picoband® IF analysis of LBR using anti-LBR antibody (A01238-2). <br>
LBR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LBR Antibody (A01238-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01238-2-lbr-primary-antibodies-fcm-testing-5.jpgAnti-Lamin B Receptor/LBR Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-LBR antibody (A01238-2). <br>Overlay histogram showing U20S cells stained with A01238-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LBR Antibody (A01238-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lmtk3-picoband-trade-antibody-a08432-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08432-2-LMTK3-primary-antibodies-WB-testing-1.jpgAnti-LMTK3 Antibody Picoband® Western blot analysis of LMTK3 using anti-LMTK3 antibody (A08432-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HEK293 whole cell lysates<br>Lane 2: human PC-3 whole cell lysates<br>Lane 3: human U2OS whole cell lysates<br>Lane 4: mouse thymus tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMTK3 antigen affinity purified polyclonal antibody (Catalog # A08432-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMTK3 at approximately 153KD. The expected band size for LMTK3 is at 153KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08432-2-LMTK3-primary-antibodies-IHC-testing-2.jpgAnti-LMTK3 Antibody Picoband® IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).<br>LMTK3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08432-2-LMTK3-primary-antibodies-IHC-testing-3.jpgAnti-LMTK3 Antibody Picoband® IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).<br>LMTK3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-lonp1-lon-picoband-trade-antibody-a03808-2-boster.html2026-03-24T05:24:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03808-2-LONP1-primary-antibodies-WB-testing-1.jpgAnti-LONP1/Lon Antibody Picoband® Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human K562 whole cell lysates<br>Lane 2: human U-87MG whole cell lysates<br>Lane 3: human PC-3 whole cell lysates<br>Lane 4: human A549 whole cell lysates<br>Lane 5: mouse liver tissue lysates<br>Lane 6: mouse kidney tissue lysates<br>Lane 7: mouse SP20 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (Catalog # A03808-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LONP1 at approximately 106KD. The expected band size for LONP1 is at 106KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03808-2-lonp1-primary-antibodies-wb-testing-2.jpgAnti-LONP1/Lon Antibody Picoband® Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human U87 whole cell lysates, <br>
Lane 3: human HEL whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (A03808-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LONP1 at approximately 106 kDa. The expected band size for LONP1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03808-2-LONP1-primary-antibodies-IHC-testing-3.jpgAnti-LONP1/Lon Antibody Picoband® IHC analysis of LONP1 using anti-LONP1 antibody (A03808-2).<br>LONP1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LONP1 Antibody (A03808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03808-2-LONP1-primary-antibodies-IHC-testing-4.jpgAnti-LONP1/Lon Antibody Picoband® IHC analysis of LONP1 using anti-LONP1 antibody (A03808-2).<br>LONP1 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LONP1 Antibody (A03808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03808-2-lonp1-primary-antibodies-ihc-testing-5.jpgAnti-LONP1/Lon Antibody Picoband® IHC analysis of LONP1 using anti-LONP1 antibody (A03808-2).<br>LONP1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LONP1 Antibody (A03808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03808-2-lonp1-primary-antibodies-if-testing-6.jpgAnti-LONP1/Lon Antibody Picoband® IF analysis of LONP1 using anti-LONP1 antibody (A03808-2). <br>
LONP1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LONP1 Antibody (A03808-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03808-2-lonp1-primary-antibodies-ip-testing-1.jpgAnti-LONP1/Lon Antibody Picoband®Immunoprecipitating (IP) LONP1 in K562 whole cell lysate.<br>
Western blot analysis of LONP1 using anti-LONP1 antibody (A03808-2); <br>
Lane 1: K562 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-LONP1 antibody in K562 whole cell lysate;<br>
Lane 3: anti-LONP1 antibody (2μg) + K562 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LONP1 antigen affinity purified polyclonal antibody (A03808-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for LONP1 at approximately 106 kDa. The expected band size for LONP1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03808-2-lonp1-primary-antibodies-fcm-testing-7.jpgAnti-LONP1/Lon Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-LONP1 antibody (A03808-2).<br>
Overlay histogram showing HL-60 cells stained with A03808-2 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LONP1 Antibody (A03808-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-loricrin-lor-picoband-trade-antibody-a05290-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05290-1-LOR-primary-antibodies-WB-testing-1.jpgAnti-Loricrin/LOR Antibody Picoband® Western blot analysis of LOR using anti-LOR antibody (A05290-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: human U-87MG whole cell lysates<br>Lane 2: human K562 whole cell lysates<br>Lane 3: human U-937 whole cell lysates<br>Lane 4: human THP-1 whole cell lysates<br>Lane 5: rat heart tissue lysates<br>Lane 6: rat small intestine tissue lysates<br>Lane 7: mouse heart tissue lysates<br>Lane 8: mouse small intestine tissue lysates<br>Lane 9: mouse Neuro-2a whole cell lysates<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOR antigen affinity purified polyclonal antibody (Catalog # A05290-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOR at approximately 26KD. The expected band size for LOR is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05290-1-LOR-primary-antibodies-IHC-testing-2.jpgAnti-Loricrin/LOR Antibody Picoband® IHC analysis of LOR using anti-LOR antibody (A05290-1).<br>LOR was detected in paraffin-embedded section of human sarcoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05290-1-LOR-primary-antibodies-IHC-testing-3.jpgAnti-Loricrin/LOR Antibody Picoband® IHC analysis of LOR using anti-LOR antibody (A05290-1).<br>LOR was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05290-1-LOR-primary-antibodies-IHC-testing-4.jpgAnti-Loricrin/LOR Antibody Picoband® IHC analysis of LOR using anti-LOR antibody (A05290-1).<br>LOR was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LOR Antibody (A05290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-mecp2-picoband-trade-antibody-a00047-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-wb-testing-1.jpgAnti-MECP2 Antibody Picoband®Western blot analysis of MECP2 using anti-MECP2 antibody (A00047). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human U2OS whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat C6 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse Neuro-2a whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MECP2 antigen affinity purified polyclonal antibody (A00047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MECP2 at approximately 75 kDa. The expected band size for MECP2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-ihc-testing-1.jpgAnti-MECP2 Antibody Picoband®IHC analysis of MECP2 using anti-MECP2 antibody (A00047). <br>MECP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MECP2 Antibody (A00047) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-ihc-testing-2.jpgAnti-MECP2 Antibody Picoband®IHC analysis of MECP2 using anti-MECP2 antibody (A00047). <br>MECP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MECP2 Antibody (A00047) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-if-testing-1.jpgAnti-MECP2 Antibody Picoband®IF analysis of MECP2 using anti-MECP2 antibody (A00047) and anti-Alpha Tubulin antibody (M03989-3). <br>MECP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MECP2 Antibody (A00047) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-if-testing-2.jpgAnti-MECP2 Antibody Picoband®IF analysis of MECP2 using anti-MECP2 antibody (A00047). <br>
MECP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MECP2 Antibody (A00047) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-if-testing-3.jpgAnti-MECP2 Antibody Picoband®IF analysis of MECP2 using anti-MECP2 antibody (A00047). <br>
MECP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MECP2 Antibody (A00047) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-if-testing-4.jpgAnti-MECP2 Antibody Picoband®IF analysis of MECP2 using anti-MECP2 antibody (A00047). <br>
MECP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MECP2 Antibody (A00047) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00047-mecp2-primary-antibodies-fcm-testing-1.jpgAnti-MECP2 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-MECP2 antibody (A00047). <br>
Overlay histogram showing 293T cells stained with A00047 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MECP2 Antibody (A00047, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mid1-picoband-trade-antibody-a01774-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01774-1-MID1-primary-antibodies-WB-testing-1.jpgAnti-MID1 Antibody Picoband® Western blot analysis of MID1 using anti-MID1 antibody (A01774-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HEK29 whole cell lysates<br>Lane 2: human Hela whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MID1 antigen affinity purified polyclonal antibody (Catalog # A01774-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MID1 at approximately 75KD. The expected band size for MID1 is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01774-1-mid1-primary-antibodies-wb-testing-2.jpgAnti-MID1 Antibody Picoband®Western blot analysis of MID1 using anti-MID1 antibody (A01774-1).<br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br>
Lane 1: mouse head tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MID1 antigen affinity purified polyclonal antibody (A01774-1) at 0.5 μg/ml overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MID1 at approximately 75 kDa.The expected band size for MID1 is at 75 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01774-1-MID1-primary-antibodies-IHC-testing-2.jpgAnti-MID1 Antibody Picoband® IHC analysis of MID1 using anti-MID1 antibody (A01774-1).<br>MID1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MID1 Antibody (A01774-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01774-1-MID1-primary-antibodies-IHC-testing-3.jpgAnti-MID1 Antibody Picoband® IHC analysis of MID1 using anti-MID1 antibody (A01774-1).<br>MID1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MID1 Antibody (A01774-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01774-1-mid1-primary-antibodies-if-testing-4.jpgAnti-MID1 Antibody Picoband® IF analysis of MID1 using anti-MID1 antibody (A01774-1). <br>
MID1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MID1 Antibody (A01774-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01774-1-mid1-primary-antibodies-if-testing-1.jpgAnti-MID1 Antibody Picoband®IF analysis of MID1 using anti-MID1 antibody (A01774-1).
MID1 was detected in a paraffin-embedded section of mouse head tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti-MID1 Antibody (A01774-1) at 10 μg/ml overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01774-1-mid1-primary-antibodies-fcm-testing-5.jpgAnti-MID1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-MID1 antibody (A01774-1).<br>Overlay histogram showing A431 cells stained with A01774-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MID1 Antibody (A01774-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mt-nd4-picoband-trade-antibody-a04180-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04180-1-MT-ND4-primary-antibodies-WB-testing-1.jpgAnti-MT-ND4 Antibody Picoband® Western blot analysis of MT-ND4 using anti-MT-ND4 antibody (A04180-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human THP-1 whole cell lysates<br>Lane 4: human Caco-2 whole cell lysates<br>Lane 5: human U2OS whole cell lysates<br>Lane 6: human A549 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND4 antigen affinity purified polyclonal antibody (Catalog # A04180-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-ND4 at approximately 36KD. The expected band size for MT-ND4 is at 52KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mtor-mtor-picoband-trade-antibody-a00003-2-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00003-2-mtor-primary-antibodies-wb-testing-1.jpgAnti-mTOR/MTOR Antibody Picoband® Western blot analysis of MTOR using anti-MTOR antibody (A00003-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: monkey COS-7 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human Jurkat whole cell ltsates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR antigen affinity purified polyclonal antibody (Catalog # A00003-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00003-2-MTOR-primary-antibodies-IHC-testing-2.jpgAnti-mTOR/MTOR Antibody Picoband® IHC analysis of MTOR using anti-MTOR antibody (A00003-2).<br>MTOR was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-muc20-picoband-trade-antibody-a07372-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07372-MUC20-primary-antibodies-WB-testing-1.jpgAnti-MUC20 Antibody Picoband® Western blot analysis of MUC20 using anti-MUC20 antibody (A07372). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HEK293 whole cell lysates<br>Lane 2: human A549 whole cell lysates<br>Lane 3: uman HL-60 whole cell lysates<br>Lane 4: uman K562 whole cell lysates<br>Lane 5: rat kidney tissue lysates<br>Lane 6: mouse kidney tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC20 antigen affinity purified polyclonal antibody (Catalog # A07372) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC20 at approximately 75KD. The expected band size for MUC20 is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07372-MUC20-primary-antibodies-IHC-testing-2.jpgAnti-MUC20 Antibody Picoband® IHC analysis of MUC20 using anti-MUC20 antibody (A07372).<br>MUC20 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUC20 Antibody (A07372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07372-MUC20-primary-antibodies-IHC-testing-3.jpgAnti-MUC20 Antibody Picoband® IHC analysis of MUC20 using anti-MUC20 antibody (A07372).<br>MUC20 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUC20 Antibody (A07372) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-enos-nos3-picoband-trade-antibody-a01604-2-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01604-2-NOS3-primary-antibodies-WB-testing-1.jpgAnti-eNOS/NOS3 Antibody Picoband® Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates<br>Lane 2: human K562 whole cell lysates<br>Lane 3: human HepG2 whole cell lysates<br>Lane 4: human THP-1 whole cell lysates<br>Lane 5: rat kidney tissue lysates<br>Lane 6: mouse kidne tissue lysates<br>Lane 7: mouse small intestine tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01604-2-NOS3-primary-antibodies-IHC-testing-2.jpgAnti-eNOS/NOS3 Antibody Picoband® IHC analysis of NOS3 using anti-NOS3 antibody (A01604-2).<br>NOS3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOS3 Antibody (A01604-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01604-2-NOS3-primary-antibodies-IHC-testing-3.jpgAnti-eNOS/NOS3 Antibody Picoband® IHC analysis of NOS3 using anti-NOS3 antibody (A01604-2).<br>NOS3 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOS3 Antibody (A01604-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01604-2-NOS3-primary-antibodies-IHC-testing-4.jpgAnti-eNOS/NOS3 Antibody Picoband® IHC analysis of NOS3 using anti-NOS3 antibody (A01604-2).<br>NOS3 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOS3 Antibody (A01604-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01604-2-NOS3-primary-antibodies-FC-testing-5.jpgAnti-eNOS/NOS3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-NOS3 antibody (A01604-2).<br>Overlay histogram showing HepG2 cells stained with A01604-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOS3 Antibody (A01604-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gpcr-gpr7-npbwr1-picoband-trade-antibody-a08247-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08247-1-NPBWR1-primary-antibodies-WB-testing-1.jpgAnti-GPCR GPR7/NPBWR1 Antibody Picoband® Western blot analysis of NPBWR1 using anti-NPBWR1 antibody (A08247-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissues lysates<br>Lane 2: human U-937 whole cell lysates<br>Lane 3: rat heart tissue lysates<br>Lane 4: mouse heart tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPBWR1 antigen affinity purified polyclonal antibody (Catalog # A08247-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPBWR1 at approximately 43KD. The expected band size for NPBWR1 is at 36KD.
https://www.bosterbio.com/products/primary-antibodies/anti-olfm4-picoband-trade-antibody-a04094-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04094-1-OLMF4-primary-antibodies-WB-testing-1.jpgAnti-OLFM4 Antibody Picoband® Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates<br>Lane 2: human U2OS whole cell lysates<br>Lane 3: human U-87MG whole cell lysates<br>Lane 4: human HL-60 whole cell lysates<br>Lane 5: human K562 whole cell lysates<br>Lane 6: human THP-1 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04094-1-OLMF4-primary-antibodies-WB-testing-2.jpgAnti-OLFM4 Antibody Picoband® Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat spleen tissue lysates<br>Lane 2: rat kidney tissue lysates<br>Lane 3: mouse kidney tissue lysates<br>Lane 4: mouse lung tissue lysates<br>Lane 5: mouse SP20 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04094-1-OLMF4-primary-antibodies-IHC-testing-3.jpgAnti-OLFM4 Antibody Picoband® IHC analysis of OLMF4 using anti-OLMF4 antibody (A04094-1).<br>OLMF4 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OLMF4 Antibody (A04094-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04094-1-OLMF4-primary-antibodies-IHC-testing-4.jpgAnti-OLFM4 Antibody Picoband® IHC analysis of OLMF4 using anti-OLMF4 antibody (A04094-1).<br>OLMF4 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OLMF4 Antibody (A04094-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-pef1-picoband-trade-antibody-a09591-2-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-WB-testing-1.jpgAnti-PEF1 Antibody Picoband® Western blot analysis of PEF1 using anti-PEF1 antibody (A09591-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HEK293 whole cell lysates<br>Lane 2: human placenta tissue lysates<br>Lane 3: human PC-3 whole cell lysates<br>Lane 4: human U-87MG whole cell lysates<br>Lane 5: human U-937 whole cell lysates<br>Lane 6: human HepG2 whole cell lysates<br>Lane 7: human A431 whole cell lysates<br>Lane 8: human U2OS whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEF1 antigen affinity purified polyclonal antibody (Catalog # A09591-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEF1 at approximately 30KD. The expected band size for PEF1 is at 30KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-WB-testing-2.jpgAnti-PEF1 Antibody Picoband® Western blot analysis of PEF1 using anti-PEF1 antibody (A09591-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat lung tissues lysates<br>Lane 2: rat testicular tissue lysates<br>Lane 3: mouse lung tissue lysates<br>Lane 4: mouse testicular tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEF1 antigen affinity purified polyclonal antibody (Catalog # A09591-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEF1 at approximately 30KD. The expected band size for PEF1 is at 30KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-IHC-testing-3.jpgAnti-PEF1 Antibody Picoband® IHC analysis of PEF1 using anti-PEF1 antibody (A09591-2).<br>PEF1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PEF1 Antibody (A09591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-IHC-testing-4.jpgAnti-PEF1 Antibody Picoband® IHC analysis of PEF1 using anti-PEF1 antibody (A09591-2).<br>PEF1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PEF1 Antibody (A09591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-IHC-testing-5.jpgAnti-PEF1 Antibody Picoband® IHC analysis of PEF1 using anti-PEF1 antibody (A09591-2).<br>PEF1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PEF1 Antibody (A09591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A09591-2-PEF1-primary-antibodies-IHC-testing-6.jpgAnti-PEF1 Antibody Picoband® IHC analysis of PEF1 using anti-PEF1 antibody (A09591-2).<br>PEF1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PEF1 Antibody (A09591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-bhc80-phf21a-antibody-a08507-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-WB-testing-1.jpgAnti-BHC80/PHF21A Antibody Picoband® Western blot analysis of PHF21A using anti-PHF21A antibody (A08507-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates<br>Lane 2: rat brain tissue lysates<br>Lane 3: mouse brain tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF21A antigen affinity purified polyclonal antibody (Catalog # A08507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF21A at approximately 75-95KD. The expected band size for PHF21A is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-ihc-testing-10_1.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1). <br> PHF21A was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IF-testing-2.jpgAnti-BHC80/PHF21A Antibody Picoband® IF analysis of PHF21A using anti-PHF21A antibody (A08507-1) <br>PHF21A was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IF-testing-3.jpgAnti-BHC80/PHF21A Antibody Picoband® IF analysis of PHF21A using anti-PHF21A antibody (A08507-1) <br>PHF21A was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-4.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-5.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-6.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human endometrial carcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-7.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-8.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08507-1-PHF21A-primary-antibodies-IHC-testing-9.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1).<br>PHF21A was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-ihc-testing-11_1.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1). <br> PHF21A was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-ihc-testing-12_1.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1). <br> PHF21A was detected in paraffin-embedded section of human sarcoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-ihc-testing-13_1.jpgAnti-BHC80/PHF21A Antibody Picoband® IHC analysis of PHF21A using anti-PHF21A antibody (A08507-1). <br> PHF21A was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHF21A Antibody (A08507-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-fc-testing-14_1.jpgAnti-BHC80/PHF21A Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-PHF21A antibody (A08507-1). <br> Overlay histogram showing HeLa cells stained with A08507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHF21A Antibody (A08507-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08507-1-phf21a-primary-antibodies-fc-testing-15_1.jpgAnti-BHC80/PHF21A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PHF21A antibody (A08507-1). <br> Overlay histogram showing SiHa cells stained with A08507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHF21A Antibody (A08507-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ret-picoband-trade-antibody-a00293-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-WB-testing-1.jpgAnti-RET Antibody Picoband® Western blot analysis of RET using anti-RET antibody (A00293-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human THP-1 whole cell lysates<br>Lane 2: human HL-60 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-2.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-3.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-4.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-5.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-6.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00293-1-RET-primary-antibodies-IHC-testing-7.jpgAnti-RET Antibody Picoband® IHC analysis of RET using anti-RET antibody (A00293-1).<br>RET was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RET Antibody (A00293-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf31-hoip-picoband-trade-antibody-a04457-3-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04457-3-RNF31-primary-antibodies-WB-testing-1.jpgAnti-RNF31/HOIP Antibody Picoband® Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissues lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human PC-3 whole cell lysates<br>Lane 4: human A549 whole cell lysates<br>Lane 5: human U2OS whole cell lysates<br>Lane 6: human Caco-2 whole cell lysates<br>Lane 7: human Hela whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf169-picoband-trade-antibody-a10407-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10407-1-rnf169-primary-antibodies-wb-testing-1.jpgAnti-RNF169 Antibody Picoband® Western blot analysis of RNF169 using anti-RNF169 antibody (A10407-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates,<br>
Lane 2: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF169 antigen affinity purified polyclonal antibody (Catalog # A10407-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF169 at approximately 90 kDa. The expected band size for RNF169 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A10407-1-RNF169-primary-antibodies-IHC-testing-2.jpgAnti-RNF169 Antibody Picoband® IHC analysis of RNF169 using anti-RNF169 antibody (A10407-1).<br>RNF169 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF169 Antibody (A10407-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A10407-1-RNF169-primary-antibodies-IHC-testing-3.jpgAnti-RNF169 Antibody Picoband® IHC analysis of RNF169 using anti-RNF169 antibody (A10407-1).<br>RNF169 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF169 Antibody (A10407-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A10407-1-RNF169-primary-antibodies-IHC-testing-4.jpgAnti-RNF169 Antibody Picoband® IHC analysis of RNF169 using anti-RNF169 antibody (A10407-1).<br>RNF169 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF169 Antibody (A10407-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A10407-1-RNF169-primary-antibodies-IHC-testing-5.jpgAnti-RNF169 Antibody Picoband® IHC analysis of RNF169 using anti-RNF169 antibody (A10407-1).<br>RNF169 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF169 Antibody (A10407-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-robo1-picoband-trade-antibody-a01530-2-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-2-robo1-primary-antibodies-wb-testing-1.jpgAnti-ROBO1 Antibody Picoband® Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human 293T whole cell lysates, <br>
Lane 4: human Jurkat whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01530-2-robo1-primary-antibodies-fcm-testing-2.jpgAnti-ROBO1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ROBO1 antibody (A01530-2). <br>
Overlay histogram showing A549 cells stained with A01530-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROBO1 Antibody (A01530-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-semaphorin-3b-sema3b-picoband-trade-antibody-a06559-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06559-1-SEMA3B-primary-antibodies-WB-testing-1.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® Western blot analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: human placenta tissue lysates<br>Lane 2: human U2OS whole cell lysates<br>Lane 3: human A549 whole cell lysates<br>Lane 4: human U-87MG whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3B antigen affinity purified polyclonal antibody (Catalog # A06559-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3B at approximately 83KD. The expected band size for SEMA3B is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06559-1-SEMA3B-primary-antibodies-WB-testing-2.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® Western blot analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat thymus tissues lysates<br>Lane 2: rat kidney tissue lysates<br>Lane 3: mouse thymus tissue lysates<br>Lane 4: mouse lung tissue lysates<br>Lane 5: mouse small intestine tissue lysates<br>Lane 6: mouse kidney tissue lysates<br>Lane 7: mouse HEPA1-6 whole cel lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3B antigen affinity purified polyclonal antibody (Catalog # A06559-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3B at approximately 83KD. The expected band size for SEMA3B is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-3.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-4.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-5.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-6.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-7.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-ihc-testing-8.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IHC analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06559-1-sema3b-primary-antibodies-if-testing-9.jpgAnti-Semaphorin 3B/SEMA3B Antibody Picoband® IF analysis of SEMA3B using anti-SEMA3B antibody (A06559-1). <br>
SEMA3B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SEMA3B Antibody (A06559-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-semaphorin-3c-sema3c-picoband-trade-antibody-a06444-1-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A06444-1-SEMA3C-primary-antibodies-WB-testing-1.jpgAnti-Semaphorin 3c/SEMA3C Antibody Picoband® Western blot analysis of SEMA3C using anti-SEMA3C antibody (A06444-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissues lysates<br>Lane 2: human A549 whole cell lysates<br>Lane 3: human U2OS whole cell lysates<br>Lane 4: rat liver tissue lysates<br>Lane 5: mouse lung tissue lysates<br>Lane 6: mouse kidney tissue lysates<br>Lane 7: mouse liver tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3C antigen affinity purified polyclonal antibody (Catalog # A06444-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3C at approximately 85KD. The expected band size for SEMA3C is at 85KD.
https://www.bosterbio.com/products/primary-antibodies/anti-nramp1-slc11a1-picoband-trade-antibody-a02547-3-boster.html2026-03-24T05:24:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02547-3-slc11a1-primary-antibodies-wb-testing-1.jpgAnti-NRAMP1/SLC11A1 Antibody Picoband®Western blot analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: mouse Hepa1/6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC11A1 antigen affinity purified polyclonal antibody (A02547-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC11A1 at approximately 120 kDa. The expected band size for SLC11A1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02547-3-SLC11A1-primary-antibodies-IHC-testing-2.jpgAnti-NRAMP1/SLC11A1 Antibody Picoband® IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).<br>SLC11A1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02547-3-SLC11A1-primary-antibodies-IHC-testing-3.jpgAnti-NRAMP1/SLC11A1 Antibody Picoband® IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).<br>SLC11A1 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02547-3-SLC11A1-primary-antibodies-IHC-testing-4.jpgAnti-NRAMP1/SLC11A1 Antibody Picoband® IHC analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3).<br>SLC11A1 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02547-3-slc11a1-primary-antibodies-if-testing-5.jpgAnti-NRAMP1/SLC11A1 Antibody Picoband® IF analysis of SLC11A1 using anti-SLC11A1 antibody (A02547-3). <br>
SLC11A1 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC11A1 Antibody (A02547-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-smad2-antibody-a00090-1-boster.html2026-03-24T05:24:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00090-1-SMAD2-primary-antibodies-WB-testing-1.jpgAnti-SMAD2 Antibody Picoband® Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates<br>Lane 2: rat liver tissue lysates<br>Lane 3: mouse testicular tissue lysates<br>Lane 4: mouse heart tissue lysates<br>Lane 5: mouse lung tissue lysates<br>Lane 6: mouse lung tissue lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-thnov11p7110g003.jpgAnti-SMAD2 Antibody Picoband®SRPX2 is elevated in fibroblasts in a TGF-β/SMADs manner. A : Western blot analysis of SRPX2 expression in HPFs following different dose of TGF-β1 induction for 24 h. B : Results for time-course Western blot analysis of SRPX2 expression in HPFs following TGF-β1 (10 ng/ml). C : Results for co-immunostaining of SRPX2 and p-SMAD2/3 in HPFs following TGF-β1 induction for 1h (up), lung sections of pulmonary fibrosis mice (middle), and lung sections from IPF patients (down). The nuclei were stained blue by DAPI, and the images were taken under original magnification ×400. D-E : Western blot (D) and RT-PCR (E) analysis of SRPX2 expression in HPFs pre-treated with SB431542 treatment following TGF-β1 induction. F-G : Western blot (F) and RT-PCR (G) analysis of SRPX2 expression in HPFs pre-treated with SIS3-HCL following TGF-β1 induction. The data are represented as the mean ± SEM of three independent experiments. ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8171094/&orig_host=www.ncbi.nlm.nih.gov'>34093874</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-thnov11p7110g004.jpgAnti-SMAD2 Antibody Picoband®SRPX2 regulated TGF-β/SMADs signaling pathways by AP1 and SMAD7. A: Results for Western blot analysis of p-SMAD2, SMAD2, p-SMAD3 and SMAD3 expression in HPFs following TGF-β1 stimulation. B-C : Western blot (B) and RT-PCR (C) analysis of SMAD7 expression in HPFs following TGF-β1 induction. D : Expression of AP1 in HPFs after TGF-β1 stimulation. E : Western blot results for analysis of the levels of P-SMAD2, P-SMAD3 and SMAD7 in HPFs pre-treated with T-5224 (an inhibitor for AP-1) treatment following TGF-β1 induction. The data are represented as the mean ± SEM of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8171094/&orig_host=www.ncbi.nlm.nih.gov'>34093874</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-thnov11p7110g007.jpgAnti-SMAD2 Antibody Picoband®Srpx2 promoted FMT in BLM-induced pulmonary fibrosis. A : Western blot analysis of Fibronectin, Col1a1, α-SMA and Srpx2 expression in mice after BLM induction with Scrambled or Srpx2 siRNA-loaded liposomes. B : Representative images of immunostaining of Fibronectin, Col1a1 and α-SMA in the mice lung sections. The nuclei were stained blue by DAPI, and the images were taken under original magnification ×400. C : Western blot analysis of p-Smad2, p-Smad3 and Smad7 expression in mice after BLM induction. D : RT-PCR analysis of AP-1 expression in mice in each group. Six mice were included in each study group. The data are represented as the mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8171094/&orig_host=www.ncbi.nlm.nih.gov'>34093874</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-thnov11p7110g008.jpgAnti-SMAD2 Antibody Picoband®A diagram for mechanisms underlying SRPX2 regulation of pulmonary fibrosis. Specifically, TGF-β enhanced SRPX2 expression in a TGFβR1 and SMAD3-dependent manner. Subsequently, SRPX2 inhibited the expression of AP-1, by which it blunt SMAD7 expression and accelerate the phosphorylation of SMAD2/3. The activated TGF-β/SMADs signaling would promote the FMT and pulmonary fibrosis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8171094/&orig_host=www.ncbi.nlm.nih.gov'>34093874</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00090-1-SMAD2-primary-antibodies-IHC-testing-2.jpgAnti-SMAD2 Antibody Picoband® IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1).<br>SMAD2 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00090-1-SMAD2-primary-antibodies-IHC-testing-3.jpgAnti-SMAD2 Antibody Picoband® IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1).<br>SMAD2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00090-1-SMAD2-primary-antibodies-IHC-testing-4.jpgAnti-SMAD2 Antibody Picoband® IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1).<br>SMAD2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00090-1-SMAD2-primary-antibodies-IHC-testing-5.jpgAnti-SMAD2 Antibody Picoband® IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1).<br>SMAD2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-smad2-primary-antibodies-if-testing-6.jpgAnti-SMAD2 Antibody Picoband® IF analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). <br>
SMAD2 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00090-1-smad2-primary-antibodies-fcm-testing-7.pngAnti-SMAD2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SMAD2 antibody (A00090-1). <br>Overlay histogram showing K562 cells stained with A00090-1 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMAD2 Antibody (A00090-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sox9-picoband-trade-antibody-a00177-2-boster.html2026-03-24T05:24:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-wb-testing-1.jpgAnti-SOX9 Antibody Picoband® Western blot analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates<br> Lane 2: human PC-3 whole cell lysates <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified polyclonal antibody (Catalog # A00177-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70KD. The expected band size for SOX9 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-jcmm-28-e70140-g001.jpgAnti-SOX9 Antibody Picoband®Hoxc10 exists in mesodermal derived callus. The immunofluorescence of Sox9 and CD44 in the mandible homotopic grafting (A), femoral heterotopic grafting (B) and femoral homotopic grafting(C). Sox9 represents cartilage (red), CD44 is a BMSCs marker (green), and DAPI marks the nucleus. (Scale bars, 500 μm) (D–F) represent the immunofluorescence of CD105 in mandible homotopic grafting, femoral heterotopic grafting, and femoral homotopic grafting, respectively. The white dotted line shows the edge of the femoral graft and the mandibular graft, and the middle of the dotted line is the callus. (Scale bars, 200 μm) (G) Localization of Hoxc10 at callus in femoral heterotopic grafting. (Scale bars, 100 μm; Scale bars, 20 μm) The data are presented as the mean ± SD ( n = 6). * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11493555/'>39434203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-jcmm-28-e70140-g006.jpgAnti-SOX9 Antibody Picoband®Hoxc10 is retained in L/M‐BMSCs in vitro. (A) Schematic of Transwell co‐culture model of L‐BMSCs and M‐BMSCs. (B) Schematic of the limb bones and mandibles from different embryonic origins. The mandible is of neural crest origin (blue) and the limb bone is of mesodermal origin (orange) (C) qPCR verified the gene expression levels of Sox9 and Col2a1 before and after co‐culture of L‐BMSCs and M‐BMSCs. (D) The proliferation, osteogenic and chondrogenic genes of femoral homotopic grafting, femoral heterotopic grafting and mandibles homotopic grafting. (E) After 21 days of chondrogenic induction in the upper layer cells of Transwell model before and after co cultivation with L‐BMSCs and M‐BMSCs, blue stained proteoglycans were observed using Alcian blue. (F) Quantitative analysis of Alizarin blue staining before and after co culture of L‐BMSCs and M‐BMSCs. The data are presented as the mean ± SD ( n = 3). * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11493555/'>39434203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-jcmm-28-e70140-g004.jpgAnti-SOX9 Antibody Picoband®Hoxc10 is positively correlated with cartilage. n (C) q‐PCR validated the expression levels of Sox9 gene after overexpression and knockout of Hoxc10. (D) q‐PCR validated the expression levels of the Col2a1 gene after overexpression and knockout of Hoxc10. (E) q‐PCR validated the expression levels of Aggrecan gene after overexpression and knockout of Hoxc10. (F) The proteoglycan of BMSCs after overexpression and knockdown Hoxc10 was observed by Alcian blue staining 21 days after chondrogenic induction. (G) Quantitative analysis of Alcian blue staining. (H) Schematic diagram of co‐culture of L‐BMSCs and M‐BMSCs with and without Hoxc10 knockout. (I) Col2a1 gene expression in L‐BMSCs after Hoxc10 knockout and co‐culture with M‐BMSCs compared to control. (J) ChIP experiment of Sox9 and Hoxc10 protein binding. The data are presented as the mean ± SD ( n = 3).* p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11493555/'>39434203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-12951_2023_2050_fig1_html.pngAnti-SOX9 Antibody Picoband®LRRK2-IN-1 suppresses the IL-1β-induced inflammation and catabolism and induces anabolism without causing the inhibition of chondrocyte viability. A Schematic diagram of cell treatment and experimental procedures. B Cell viability assessed by CCK8 assay. No obvious inhibition of chondrocyte proliferation was observed when treated with 0.5, 1.0, 2.5, and 5.0 µM LRRK2-IN-1 for 24 h. Data represent mean ± SD; N = 6/group; one-way ANOVA; ns, not significant. C Western blot analyses of the protein levels of anabolic, catabolic, inflammatory factors in the IL-1β-induced chondrocytes treated with 0.5, 1.0, 2.5, and 5.0 µM LRRK2-IN-1 for 24 h. LRRK2-IN-1 suppressed MMP3, MMP13, iNOS, and COX2 and induced COL2 and SOX9 in a dose-dependent manner. D Quantitative analyses of the western blot results. Data represent mean ± SD; N = 3/group; *P < 0.05; **P < 0.01 by one-way ANOVA. E Immunofluorescence of iNOS, MMP13, and aggrecan expression in the IL-1β-induced chondrocytes treated with 5.0 µM LRRK2-IN-1 for 24 h. Scar bar: 400 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02050-7'>37605203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-12951_2023_2050_fig3_html.pngAnti-SOX9 Antibody Picoband®W-Exo-L@GelMA exhibits a strong chondrocyte-targeting effect and a pronounced action on promoting anabolism and suppressing catabolism and inflammation without causing the inhibition of chondrocyte viability. A Cell viability assessed by CCK8 assay. No obvious cytotoxicity on chondrocytes was observed when treated with W-Exo-L@GelMA loaded with 0.5, 1.0, 2.5, and 5.0 µM LRRK2-IN-1 for 48 h. Data represent mean ± SD; N = 6/group; one-way ANOVA; ns, not significant. B Immunofluorescence of Dil-labeled exosomes. The uptake of exosomes was observed in the chondrocytes when treated with Exo-L, Exo-L@GelMA or W-Exo-L@GelMA for 48 h. Dil was used for labeling exosomes (red), DAPI to label nuclei (blue), and Phalloidin to label the cytoskeleton (green). Scar bar: 200 μm. C Western blot analyses of the protein levels of anabolic, catabolic, and inflammatory factors in the IL-1β-induced chondrocytes treated with W-Exo-L@GelMA loaded with 0.5, 1.0, 2.5, and 5.0 µM LRRK2-IN-1 for 48 h. W-Exo-L@GelMA promoted COL2 and SOX9 and inhibited iNOS, COX2, MMP3, and MMP13 protein levels in a dose-dependent manner. D Quantitative analysis of the western blot results. Data represent mean ± SD; N = 3/group; *P < 0.05; **P < 0.01 by one-way ANOVA <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02050-7'>37605203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-13287_2019_1404_fig4_html.pngAnti-SOX9 Antibody Picoband®Antioxidants preserve ADSC cell stemness and multidirectional differentiation potential during long-term in vitro expansion. After treatment with 10 μM GSH or melatonin, the ADSCs cultured for passage 3 (P3), passage 6 (P6), and passage 9 (P9) were used in the following analysis. a Osteogenesis differentiation of passaged ADSCs (Alizarin Red S staining; scale bar, 50 μm). b Adipogenesis differentiation of passaged ADSCs (Oil Red O staining; scale bar, 50 μm). c Western blot analysis for RUNX-2 in osteogenic cells. d Western blot analysis for perilipin A in adipogenic cells. e Chondrogenesis differentiation of passaged ADSCs (Alcian blue staining; scale bar, 50 μm). f Western blot analysis for SOX-9 in chondrogenic cells. g Western blot analysis for SOX-2, OCT-4, and β-actin in ADSCs. ADSCs, adipose tissue-derived stem cells; GSH, reduced glutathione <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1404-9'>31623678</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-2.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-3.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-4.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-5.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-6.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-7.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-8.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00177-2-sox9-primary-antibodies-ihc-testing-9.jpgAnti-SOX9 Antibody Picoband® IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2). <br> SOX9 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-sox15-picoband-trade-antibody-a07722-1-boster.html2026-03-24T05:24:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-WB-testing-1.jpgAnti-SOX15 Antibody Picoband® Western blot analysis of SOX15 using anti-SOX15 antibody (A07722-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A431 whole cell lysates<br>Lane 2: human U-87MG whole cell lysates<br>Lane 3: human HEK293 whole cell lysates<br>Lane 4: human K562 whole cell lysates<br>Lane 5: human U2OS whole cell lysates<br>Lane 6: rat thymus tissue lysates<br>Lane 7: mouse SP20 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX15 antigen affinity purified polyclonal antibody (Catalog # A07722-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX15 at approximately 29KD. The expected band size for SOX15 is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-IF-testing-2.jpgAnti-SOX15 Antibody Picoband® IF analysis of SOX15 using anti-SOX15 antibody (A07722-1). <br> SOX15 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SOX15 Antibody (A07722-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-IHC-testing-3.jpgAnti-SOX15 Antibody Picoband® IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).<br>SOX15 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX15 Antibody (A07722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-IHC-testing-4.jpgAnti-SOX15 Antibody Picoband® IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).<br>SOX15 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX15 Antibody (A07722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-IHC-testing-5.jpgAnti-SOX15 Antibody Picoband® IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).<br>SOX15 was detected in paraffin-embedded section of human testis cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX15 Antibody (A07722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A07722-1-SOX15-primary-antibodies-IHC-testing-6.jpgAnti-SOX15 Antibody Picoband® IHC analysis of SOX15 using anti-SOX15 antibody (A07722-1).<br>SOX15 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX15 Antibody (A07722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-tdrd3-picoband-trade-antibody-a08978-boster.html2026-03-24T05:24:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08978-tdrd3-primary-antibodies-wb-testing-1.jpgAnti-TDRD3 Antibody Picoband® Western blot analysis of TDRD3 using anti-TDRD3 antibody (A08978). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HL-60 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human PC-3 whole cell lysates, <br> Lane 5: human HEK293 whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human A431 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD3 antigen affinity purified polyclonal antibody (Catalog # A08978) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for TDRD3 at approximately 65-73KD. The expected band size for TDRD3 is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08978-TDRD3-primary-antibodies-WB-testing-2.jpgAnti-TDRD3 Antibody Picoband® Western blot analysis of TDRD3 using anti-TDRD3 antibody (A08978). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissues lysates<br>Lane 2: rat liver tissue lysates<br>Lane 3: mouse brain tissue lysates<br>Lane 4: mouse lung tissue lysates<br>Lane 5: mouse liver tissue lysates<br>Lane 6: mouse NIH3T3 whole cell lysates <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD3 antigen affinity purified polyclonal antibody (Catalog # A08978) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDRD3 at approximately 65-73KD. The expected band size for TDRD3 is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08978-tdrd3-primary-antibodies-ihc-testing-3.jpgAnti-TDRD3 Antibody Picoband® IHC analysis of TDRD3 using anti-TDRD3 antibody (A08978). <br> TDRD3 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08978-tdrd3-primary-antibodies-ihc-testing-4.jpgAnti-TDRD3 Antibody Picoband® IHC analysis of TDRD3 using anti-TDRD3 antibody (A08978). <br> TDRD3 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08978-tdrd3-primary-antibodies-ihc-testing-5.jpgAnti-TDRD3 Antibody Picoband® IHC analysis of TDRD3 using anti-TDRD3 antibody (A08978). <br> TDRD3 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08978-tdrd3-primary-antibodies-if-testing-6.jpgAnti-TDRD3 Antibody Picoband® IF analysis of TDRD3 using anti-TDRD3 antibody (A08978) <br> TDRD3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-TDRD3 Antibody (A08978) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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https://www.bosterbio.com/picokine-elisa-kits/human-il-17rd-picokine-elisa-kit-ek2083-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2083.pngHuman IL-17RD ELISA Kit PicoKine®Human IL-17RD PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-fgfr4-picokine-elisa-kit-ek2084-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2084.pngMouse FGFR4 ELISA Kit PicoKine®Mouse FGFR4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/8-isoprostane-elisa-kit-ek7123-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7123-elisa-image-1.jpg8-Isoprostane ELISA kitPlate Setup: Each plate must contain a minimum of three blank wells (BL), three maximum binding wells (BO), and a six point standard curve (S1-S6). Each sample should be assayed in triplicate. A suggested plate format is shown:https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7123-elisa-image-2.jpg8-Isoprostane ELISA kitThe data shown here is an example of typical results obtained using 8-isoprostane ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time.
https://www.bosterbio.com/picokine-elisa-kits/prostaglandin-e2-pge2-elisa-kit-ek7124-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7124-elisa-image-1.jpgProstaglandin E2 PGE2 ELISA kitPlate Setup: Each plate must contain a minimum of three blank wells (BL), three maximum binding wells (BO), and a six point standard curve (S1-S6). Each sample should be assayed in triplicate. A suggested plate format is shown https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7124-elisa-image-2_1.jpgProstaglandin E2 PGE2 ELISA kitThe data shown here is an example of typical results obtained using the Boster PGE2 ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.059 BO wells = 1.728
https://www.bosterbio.com/picokine-elisa-kits/bisphenol-s-bps-elisa-kit-ek7125-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7125-elisa-image.jpgBisphenol S (BPS) ELISA KitThe data shown here is an example of typical results obtained using the Boster BPS ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.045 BO wells = 1.957
https://www.bosterbio.com/picokine-elisa-kits/beta-glucuronidase-20-hete-elisa-kit-ek7126-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7126-elisa-image.jpgBeta-Glucuronidase 20-HETE ELISA KitThe data shown here is an example of typical results obtained using 20-HETE ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.063 BO wells = 1.667
https://www.bosterbio.com/picokine-elisa-kits/14-15-dhet-elisa-kit-ek7127-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7127-elisa-image.jpg14,15-DHET ELISA KitThe data shown here is an example of typical results obtained using 14,15-DHET ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.059 BO wells = 2.525
https://www.bosterbio.com/picokine-elisa-kits/bisphenol-a-bpa-elisa-kit-ek7128-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7128-elisa-image.jpgBisphenol A (BPA) ELISA kitThe data shown here is an example of typical results obtained using BPA ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.052 BO wells = 2.870
https://www.bosterbio.com/picokine-elisa-kits/11-12-eet-dhet-elisa-kit-ek7129-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek7129-elisa-image.jpg11,12-EET/DHET ELISA KitThe data shown here is an example of typical results obtained using the 11,12-DHET ELISA kit. These results are only a guideline, and should not be used to determine values from your samples. The user must run their own standard curve every time. BL wells = 0.066 BO wells = 1.337
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-angiopoietin-1-picokine-reg-quick-elisa-kit-fek0559-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0559.pngHuman Angiopoietin-1 PicoKine® Quick ELISA KitHuman Angiopoietin-1 PicoKine Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-adiponectin-picokine-trade-quick-elisa-kit-fek0595-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0595_2.pngHuman Adiponectin PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-adiponectin-picokine-trade-quick-elisa-kit-fek0596-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0596_1.pngMouse Adiponectin PicoKine® Quick ELISA KitMouse Adiponectin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-adiponectin-picokine-trade-quick-elisa-kit-fek1327-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1327.pngRat Adiponectin PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-se-selectin-picokine-trade-quick-elisa-kit-fek0502-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0502_1-ELISA-mouse-se-selectin-picokine-elisa-kit.pngMouse sE-Selectin PicoKine® Quick ELISA KitMouse sE-Selectin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-se-selectin-picokine-trade-quick-elisa-kit-fek0936-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0936-2-ELISA-rat-se-selectin-picokine-elisa-kit.jpgRat sE-Selectin PicoKine® Quick ELISA KitRat sE-Selectin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-angiostatin-k1-3-picokine-trade-quick-elisa-kit-fek0905-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0905_1.pngHuman Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA KitHuman Angiostatin K1-3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-p-selectin-picokine-trade-quick-elisa-kit-fek0505-boster.html2026-03-24T05:24:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0505_1.pngHuman P-Selectin / CD62P PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-p-selectin-picokine-trade-quick-elisa-kit-fek0506-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0506.pngMouse P-Selectin / CD62P PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl5-ena-78-picokine-trade-quick-elisa-kit-fek0919-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0919_1.pngMouse CXCL5/LIX/ENA-78/GCP 2 PicoKine® Quick ELISA KitMouse CXCL5 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-aggrecan-picokine-trade-quick-elisa-kit-fek0909-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0909_1.pngHuman Aggrecan/ACAN PicoKine® Quick ELISA KitHuman Aggrecan PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-scgb1a1-uteroglobin-picokine-trade-quick-elisa-kit-fek1636-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1636.pngRat SCGB1A1/uteroglobin PicoKine® Quick ELISA KitRat SCGB1A1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd5l-picokine-trade-quick-elisa-kit-fek1413-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1413.pngHuman CD5L/CT-2 PicoKine® Quick ELISA KitHuman CD5L PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd5l-picokine-trade-quick-elisa-kit-fek1414-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1414.pngMouse CD5L/CT-2 PicoKine® Quick ELISA KitMouse CD5L PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-resistin-picokine-trade-quick-elisa-kit-fek0581-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0581_1.pngHuman Resistin PicoKine® Quick ELISA KitHuman Resistin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-resistin-picokine-trade-quick-elisa-kit-fek0582-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0582-ELISA-mouse-resistin-picokine-elisa-kit.pngMouse Resistin PicoKine® Quick ELISA KitMouse Resistin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-il-2-picokine-trade-quick-elisa-kit-fek0398-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0398.pngMouse IL-2/Interleukin-2 PicoKine® Quick ELISA KitMouse IL-2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-gdnf-picokine-trade-quick-elisa-kit-fek0362-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0362_1.pngHuman GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA KitHuman GDNF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-gdnf-picokine-trade-quick-elisa-kit-fek0363-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0363_1.pngRat GDNF PicoKine® Quick ELISA KitRat GDNF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-gdnf-picokine-trade-quick-elisa-kit-fek0935-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0935.pngMouse GDNF PicoKine® Quick ELISA KitMouse GDNF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-gm-csf-picokine-trade-quick-elisa-kit-fek0364-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0364_1.pngHuman GM-CSF PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-gm-csf-picokine-trade-quick-elisa-kit-fek0365-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0365.pngMouse GM-CSF PicoKine® Quick ELISA KitMouse GM-CSF PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-plat-tpa-picokine-trade-quick-elisa-kit-fek0897-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0897.pngHuman PLAT/TPA PicoKine® Quick ELISA KitHuman PLAT/TPA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-vegf-picokine-trade-quick-elisa-kit-fek0541-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0541_1.pngMouse VEGF PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-il-15-picokine-trade-quick-elisa-kit-fek0426-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0426.pngHuman IL-15/Interleukin-15 PicoKine® Quick ELISA KitHuman IL-15 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-clusterin-picokine-trade-quick-elisa-kit-fek0914-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0914.pngHuman Clusterin PicoKine® Quick ELISA KitHuman Clusterin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-timp-1-picokine-trade-quick-elisa-kit-fek0520-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0520_1_1.pngHuman TIMP-1 PicoKine® Quick ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-timp-2-picokine-trade-quick-elisa-kit-fek0522-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0522-2-ELISA-human-timp-2-picokine-elisa-kit.jpgHuman TIMP-2 PicoKine® Quick ELISA KitHuman TIMP-2 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-epcam-picokine-trade-quick-elisa-kit-fek0755-boster.html2026-03-24T05:24:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0755_2-ELISA-human-epcam-trop1-picokine-elisa-kit.pngHuman EPCAM/Trop1 PicoKine® Quick ELISA KitHuman EPCAM PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-opg-picokine-trade-quick-elisa-kit-fek0481-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0481.pngMouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine® Quick ELISA KitMouse OPG (TNFRSF11B) PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-fibronectin-picokine-trade-quick-elisa-kit-fek0351-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0351.pngMouse Fibronectin PicoKine® Quick ELISA KitMouse <a href="https://www.bosterbio.com/human-fibronectin-picokine-trade-elisa-kit-ek0349-boster.html" style="color:#EA8D28">Fibronectin PicoKine</a> ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-fibronectin-picokine-trade-quick-elisa-kit-fek0350-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0350.pngRat Fibronectin PicoKine® Quick ELISA KitRat Fibronectin PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd163-picokine-trade-quick-elisa-kit-fek0715-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0663.pngHuman CD163/M130 PicoKine® Quick ELISA KitHuman CD163 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-tgfbr3-picokine-trade-quick-elisa-kit-fek1383-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1383_1.pngHuman TGFBR3/Tgf Beta Riii PicoKine® Quick ELISA KitHuman TGFBR3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-il-5-picokine-trade-quick-elisa-kit-fek0407-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0407.pngHuman IL-5/Interleukin-5 PicoKine® Quick ELISA KitHuman IL-5 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-tim-3-havcr2-picokine-quick-elisa-kit-fek1644-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/K/EK1644-TIM-3-elisa-kit.jpgHuman TIM-3/HAVCR2 PicoKine® Quick ELISA KitHuman TIM-3 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-epo-picokine-trade-quick-elisa-kit-fek1351-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1351.pngRat EPO/Erythropoietin PicoKine® Quick ELISA KitRat EPO PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-il1rl1-st2-picokine-trade-quick-elisa-kit-fek1116-boster.html2026-03-24T05:24:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1116.pngHuman IL-1 RL1 / ST2 PicoKine® Quick ELISA KitHuman IL1RL1/ST2 PicoKine ELISA Kit standard curve
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https://www.bosterbio.com/products/cell-based-elisa-kits/keratin-14-colorimetric-cell-based-elisa-ekc1610-boster.html2026-03-24T05:24:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKeratin 14 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1610-keratin-14-colorimetric-cell-based-elisa-wb-testing-1.jpgKeratin 14 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/presenilin-1-colorimetric-cell-based-elisa-ekc1612-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPresenilin 1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1612-presenilin-1-colorimetric-cell-based-elisa-wb-testing-1.jpgPresenilin 1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse heart cells
https://www.bosterbio.com/products/cell-based-elisa-kits/xrcc6-colorimetric-cell-based-elisa-ekc1613-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngXRCC6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1613-xrcc6-colorimetric-cell-based-elisa-wb-testing-1.jpgXRCC6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/jmy-colorimetric-cell-based-elisa-ekc1614-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngJMY Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1614-jmy-colorimetric-cell-based-elisa-wb-testing-1.jpgJMY Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nkx6-3-colorimetric-cell-based-elisa-ekc1615-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNKX6.3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1615-nkx6-3-colorimetric-cell-based-elisa-wb-testing-1.jpgNKX6.3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562/COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pdlim1-colorimetric-cell-based-elisa-ekc1616-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPDLIM1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1616-pdlim1-colorimetric-cell-based-elisa-wb-testing-1.jpgPDLIM1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205/293/HepG2/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/taf4-colorimetric-cell-based-elisa-ekc1617-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTAF4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1617-taf4-colorimetric-cell-based-elisa-wb-testing-1.jpgTAF4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lhr2a-colorimetric-cell-based-elisa-ekc1618-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLHR2A Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1618-lhr2a-colorimetric-cell-based-elisa-wb-testing-1.jpgLHR2A Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/trim38-colorimetric-cell-based-elisa-ekc1619-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRIM38 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1619-trim38-colorimetric-cell-based-elisa-wb-testing-1.jpgTRIM38 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO/RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dlgp1-colorimetric-cell-based-elisa-ekc1620-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDLGP1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1620-dlgp1-colorimetric-cell-based-elisa-wb-testing-1.jpgDLGP1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tp53i11-colorimetric-cell-based-elisa-ekc1621-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTP53I11 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1621-tp53i11-colorimetric-cell-based-elisa-wb-testing-1.jpgTP53I11 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ovol1-colorimetric-cell-based-elisa-ekc1622-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngOVOL1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1622-ovol1-colorimetric-cell-based-elisa-wb-testing-1.jpgOVOL1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562/A549 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/klf11-colorimetric-cell-based-elisa-ekc1625-boster.html2026-03-24T05:24:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKLF11 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1625-klf11-colorimetric-cell-based-elisa-wb-testing-1.jpgKLF11 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/Jurkat/HuvEc cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/trim24-colorimetric-cell-based-elisa-ekc1627-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRIM24 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1627-trim24-colorimetric-cell-based-elisa-wb-testing-1.jpgTRIM24 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tri18-colorimetric-cell-based-elisa-ekc1628-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRI18 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1628-tri18-colorimetric-cell-based-elisa-wb-testing-1.jpgTRI18 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/gsc2-colorimetric-cell-based-elisa-ekc1629-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGSC2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1629-gsc2-colorimetric-cell-based-elisa-wb-testing-1.jpgGSC2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/HepG2/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/clock-colorimetric-cell-based-elisa-ekc1630-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngClock Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1630-clock-colorimetric-cell-based-elisa-wb-testing-1.jpgClock Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/m3k13-colorimetric-cell-based-elisa-ekc1631-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngM3K13 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1631-m3k13-colorimetric-cell-based-elisa-wb-testing-1.jpgM3K13 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HuvEc/HepG2 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/akap10-colorimetric-cell-based-elisa-ekc1633-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP10 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1633-akap10-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP10 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse brain cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akap8-colorimetric-cell-based-elisa-ekc1634-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP8 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1634-akap8-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP8 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse heart/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mast3-colorimetric-cell-based-elisa-ekc1635-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAST3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1635-mast3-colorimetric-cell-based-elisa-wb-testing-1.jpgMAST3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/zeb2-colorimetric-cell-based-elisa-ekc1636-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZEB2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1636-zeb2-colorimetric-cell-based-elisa-wb-testing-1.jpgZEB2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dlx3-colorimetric-cell-based-elisa-ekc1637-boster.html2026-03-24T05:24:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDLX3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1637-dlx3-colorimetric-cell-based-elisa-wb-testing-1.jpgDLX3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/zic1-2-3-colorimetric-cell-based-elisa-ekc1638-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZIC1/2/3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1638-zic1-2-3-colorimetric-cell-based-elisa-wb-testing-1.jpgZIC1/2/3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hnrnp-q-colorimetric-cell-based-elisa-ekc1639-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pnghnRNP Q Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1639-hnrnp-q-colorimetric-cell-based-elisa-wb-testing-1.jpghnRNP Q Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HuvEc/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sdcg1-colorimetric-cell-based-elisa-ekc1640-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSDCG1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1640-sdcg1-colorimetric-cell-based-elisa-wb-testing-1.jpgSDCG1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lmx1b-colorimetric-cell-based-elisa-ekc1641-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLMX1B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1641-lmx1b-colorimetric-cell-based-elisa-wb-testing-1.jpgLMX1B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/293/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/musculin-colorimetric-cell-based-elisa-ekc1642-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMusculin Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1642-musculin-colorimetric-cell-based-elisa-wb-testing-1.jpgMusculin Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dyskerin-colorimetric-cell-based-elisa-ekc1643-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDyskerin Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1643-dyskerin-colorimetric-cell-based-elisa-wb-testing-1.jpgDyskerin Colorimetric Cell-Based ELISAWestern blot analysis of extracts from JurKat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pfdn1-colorimetric-cell-based-elisa-ekc1644-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPFDN1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1644-pfdn1-colorimetric-cell-based-elisa-wb-testing-1.jpgPFDN1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tbx10-colorimetric-cell-based-elisa-ekc1645-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTBX10 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1645-tbx10-colorimetric-cell-based-elisa-wb-testing-1.jpgTBX10 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/trim3-colorimetric-cell-based-elisa-ekc1646-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRIM3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1646-trim3-colorimetric-cell-based-elisa-wb-testing-1.jpgTRIM3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lat3-colorimetric-cell-based-elisa-ekc1647-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLAT3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1647-lat3-colorimetric-cell-based-elisa-wb-testing-1.jpgLAT3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tacc1-colorimetric-cell-based-elisa-ekc1648-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTACC1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1648-tacc1-colorimetric-cell-based-elisa-wb-testing-1.jpgTACC1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/maf-colorimetric-cell-based-elisa-ekc1649-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMaf Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1649-maf-colorimetric-cell-based-elisa-wb-testing-1.jpgMaf Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/HepG2/HeLa/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ada2l-colorimetric-cell-based-elisa-ekc1650-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADA2L Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1650-ada2l-colorimetric-cell-based-elisa-wb-testing-1.jpgADA2L Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/taf5l-colorimetric-cell-based-elisa-ekc1651-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTAF5L Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1651-taf5l-colorimetric-cell-based-elisa-wb-testing-1.jpgTAF5L Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29/COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/bcar3-colorimetric-cell-based-elisa-ekc1652-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBCAR3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1652-bcar3-colorimetric-cell-based-elisa-wb-testing-1.jpgBCAR3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tall-2-colorimetric-cell-based-elisa-ekc1653-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTALL-2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1653-tall-2-colorimetric-cell-based-elisa-wb-testing-1.jpgTALL-2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tusc2-colorimetric-cell-based-elisa-ekc1654-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTUSC2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1654-tusc2-colorimetric-cell-based-elisa-wb-testing-1.jpgTUSC2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dgki-colorimetric-cell-based-elisa-ekc1655-boster.html2026-03-24T05:24:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDGKI Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1655-dgki-colorimetric-cell-based-elisa-wb-testing-1.jpgDGKI Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fir-colorimetric-cell-based-elisa-ekc1656-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFIR Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1656-fir-colorimetric-cell-based-elisa-wb-testing-1.jpgFIR Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/alx3-colorimetric-cell-based-elisa-ekc1657-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngALX3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1657-alx3-colorimetric-cell-based-elisa-wb-testing-1.jpgALX3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnfsf15-colorimetric-cell-based-elisa-ekc1658-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNFSF15 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1658-tnfsf15-colorimetric-cell-based-elisa-wb-testing-1.jpgTNFSF15 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf265-colorimetric-cell-based-elisa-ekc1659-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF265 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1659-znf265-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF265 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/med26-colorimetric-cell-based-elisa-ekc1660-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMED26 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1660-med26-colorimetric-cell-based-elisa-wb-testing-1.jpgMED26 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/six6-colorimetric-cell-based-elisa-ekc1661-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSIX6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1661-six6-colorimetric-cell-based-elisa-wb-testing-1.jpgSIX6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cbx6-colorimetric-cell-based-elisa-ekc1662-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBX6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1662-cbx6-colorimetric-cell-based-elisa-wb-testing-1.jpgCBX6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/gas41-colorimetric-cell-based-elisa-ekc1663-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGAS41 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1663-gas41-colorimetric-cell-based-elisa-wb-testing-1.jpgGAS41 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse lung cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tbx15-18-colorimetric-cell-based-elisa-ekc1664-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTBX15/18 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1664-tbx15-18-colorimetric-cell-based-elisa-wb-testing-1.jpgTBX15/18 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mbd3-colorimetric-cell-based-elisa-ekc1665-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMBD3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1665-mbd3-colorimetric-cell-based-elisa-wb-testing-1.jpgMBD3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pttg1-colorimetric-cell-based-elisa-ekc1666-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPTTG1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1666-pttg1-colorimetric-cell-based-elisa-wb-testing-1.jpgPTTG1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/Jurkat/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/actl6a-colorimetric-cell-based-elisa-ekc1667-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngACTL6A Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1667-actl6a-colorimetric-cell-based-elisa-wb-testing-1.jpgACTL6A Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/thrb-colorimetric-cell-based-elisa-ekc1668-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTHRB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1668-thrb-colorimetric-cell-based-elisa-wb-testing-1.jpgTHRB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/aldoa-colorimetric-cell-based-elisa-ekc1669-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngALDOA Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1669-aldoa-colorimetric-cell-based-elisa-wb-testing-1.jpgALDOA Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549/HeLa/HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fes-colorimetric-cell-based-elisa-ekc1670-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFES Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1670-fes-colorimetric-cell-based-elisa-wb-testing-1.jpgFES Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hexb-colorimetric-cell-based-elisa-ekc1671-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHEXB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1671-hexb-colorimetric-cell-based-elisa-wb-testing-1.jpgHEXB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnr16-colorimetric-cell-based-elisa-ekc1672-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNR16 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1672-tnr16-colorimetric-cell-based-elisa-ekc1672-wb-testing-1.jpgTNR16 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dna-polymerase-alpha-colorimetric-cell-based-elisa-ekc1673-boster.html2026-03-24T05:24:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDNA Polymerase alpha Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1673-dna-polymerase-alpha-colorimetric-cell-based-elisa-wb-testing-1.jpgDNA Polymerase alpha Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/293/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hkr1-colorimetric-cell-based-elisa-ekc1674-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHKR1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1674-hkr1-colorimetric-cell-based-elisa-wb-testing-1.jpgHKR1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nr2f6-colorimetric-cell-based-elisa-ekc1675-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNR2F6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1675-nr2f6-colorimetric-cell-based-elisa-wb-testing-1.jpgNR2F6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HuvEc/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/kap0-colorimetric-cell-based-elisa-ekc1676-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKAP0 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1676-kap0-colorimetric-cell-based-elisa-wb-testing-1.jpgKAP0 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/glu2b-colorimetric-cell-based-elisa-ekc1677-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGLU2B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1677-glu2b-colorimetric-cell-based-elisa-wb-testing-1.jpgGLU2B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/293/HuvEC cells
https://www.bosterbio.com/products/cell-based-elisa-kits/irf2-colorimetric-cell-based-elisa-ekc1678-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRF2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1678-irf2-colorimetric-cell-based-elisa-wb-testing-1.jpgIRF2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf24-colorimetric-cell-based-elisa-ekc1679-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF24 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1679-znf24-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF24 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hoxb9-colorimetric-cell-based-elisa-ekc1680-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHOXB9 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1680-hoxb9-colorimetric-cell-based-elisa-wb-testing-1.jpgHOXB9 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ckmt2-colorimetric-cell-based-elisa-ekc1681-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKMT2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1681-ckmt2-colorimetric-cell-based-elisa-wb-testing-1.jpgCKMT2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/kapc-a-b-colorimetric-cell-based-elisa-ekc1682-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKAPC A/B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1682-kapc-a-b-colorimetric-cell-based-elisa-wb-testing-1.jpgKAPC A/B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cebpb-colorimetric-cell-based-elisa-ekc1683-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCEBPB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1683-cebpb-colorimetric-cell-based-elisa-wb-testing-1.jpgCEBPB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/spi1-colorimetric-cell-based-elisa-ekc1684-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSPI1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1684-spi1-colorimetric-cell-based-elisa-wb-testing-1.jpgSPI1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/egr1-2-colorimetric-cell-based-elisa-ekc1685-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGR1/2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1685-egr1-2-colorimetric-cell-based-elisa-wb-testing-1.jpgEGR1/2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adrenergic-receptor-alpha-2c-colorimetric-cell-based-elisa-ekc1686-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAdrenergic Receptor alpha-2C Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1686-adrenergic-receptor-alpha-2c-colorimetric-cell-based-elisa-wb-testing-1.jpgAdrenergic Receptor alpha-2C Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tspan8-colorimetric-cell-based-elisa-ekc1687-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTSPAN8 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1687-tspan8-colorimetric-cell-based-elisa-wb-testing-1.jpgTSPAN8 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnf-receptor-ii-colorimetric-cell-based-elisa-ekc1688-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNF Receptor II Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1688-tnf-receptor-ii-colorimetric-cell-based-elisa-wb-testing-1.jpgTNF Receptor II Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7/HT-29/LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdc2l1-colorimetric-cell-based-elisa-ekc1689-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDC2L1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1689-cdc2l1-colorimetric-cell-based-elisa-wb-testing-1.jpgCDC2L1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/crp1-colorimetric-cell-based-elisa-ekc1690-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCRP1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1690-crp1-colorimetric-cell-based-elisa-wb-testing-1.jpgCRP1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/COLO/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/kapcb-colorimetric-cell-based-elisa-ekc1691-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKAPCB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1691-kapcb-colorimetric-cell-based-elisa-wb-testing-1.jpgKAPCB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/myf6-colorimetric-cell-based-elisa-ekc1692-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMYF6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1692-myf6-colorimetric-cell-based-elisa-wb-testing-1.jpgMYF6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ip3ka-colorimetric-cell-based-elisa-ekc1693-boster.html2026-03-24T05:24:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIP3KA Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1693-ip3ka-colorimetric-cell-based-elisa-wb-testing-1.jpgIP3KA Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dgka-colorimetric-cell-based-elisa-ekc1694-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDGKA Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1694-dgka-colorimetric-cell-based-elisa-wb-testing-1.jpgDGKA Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akap5-colorimetric-cell-based-elisa-ekc1695-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1695-akap5-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/HeLa/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/arbk1-colorimetric-cell-based-elisa-ekc1696-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngARBK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1696-arbk1-colorimetric-cell-based-elisa-wb-testing-1.jpgARBK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nfyb-colorimetric-cell-based-elisa-ekc1697-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNFYB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1697-nfyb-colorimetric-cell-based-elisa-wb-testing-1.jpgNFYB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mark3-colorimetric-cell-based-elisa-ekc1698-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMARK3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1698-mark3-colorimetric-cell-based-elisa-wb-testing-1.jpgMARK3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/arnt-colorimetric-cell-based-elisa-ekc1699-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngARNT Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1699-arnt-colorimetric-cell-based-elisa-wb-testing-1.jpgARNT Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/apex1-colorimetric-cell-based-elisa-ekc1700-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAPEX1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1700-apex1-colorimetric-cell-based-elisa-wb-testing-1.jpgAPEX1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/calr-colorimetric-cell-based-elisa-ekc1701-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCALR Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1701-calr-colorimetric-cell-based-elisa-wb-testing-1.jpgCALR Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/p42-mapk-colorimetric-cell-based-elisa-ekc1702-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp42 MAPK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1702-p42-mapk-colorimetric-cell-based-elisa-wb-testing-1.jpgp42 MAPK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ercc5-colorimetric-cell-based-elisa-ekc1703-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngERCC5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1703-ercc5-colorimetric-cell-based-elisa-wb-testing-1.jpgERCC5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ltk-colorimetric-cell-based-elisa-ekc1704-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLTK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1704-ltk-colorimetric-cell-based-elisa-wb-testing-1.jpgLTK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ppif-colorimetric-cell-based-elisa-ekc1705-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPPIF Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1705-ppif-colorimetric-cell-based-elisa-wb-testing-1.jpgPPIF Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/HeLa/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hoxa7-colorimetric-cell-based-elisa-ekc1706-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHOXA7 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1706-hoxa7-colorimetric-cell-based-elisa-wb-testing-1.jpgHOXA7 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/kap1-colorimetric-cell-based-elisa-ekc1707-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKAP1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1707-kap1-colorimetric-cell-based-elisa-wb-testing-1.jpgKAP1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/elf1-colorimetric-cell-based-elisa-ekc1708-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngELF1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1708-elf1-colorimetric-cell-based-elisa-wb-testing-1.jpgELF1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/grk5-colorimetric-cell-based-elisa-ekc1709-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRK5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1709-grk5-colorimetric-cell-based-elisa-wb-testing-1.jpgGRK5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/recoverin-colorimetric-cell-based-elisa-ekc1710-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRecoverin Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1710-recoverin-colorimetric-cell-based-elisa-wb-testing-1.jpgRecoverin Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hoxd12-colorimetric-cell-based-elisa-ekc1711-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHOXD12 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1711-hoxd12-colorimetric-cell-based-elisa-wb-testing-1.jpgHOXD12 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/HucEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/actinin-alpha-2-3-colorimetric-cell-based-elisa-ekc1712-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngActinin alpha-2/3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1712-actinin-alpha-2-3-colorimetric-cell-based-elisa-wb-testing-1.jpgActinin alpha-2/3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/HeLa/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cd147-colorimetric-cell-based-elisa-ekc1713-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD147 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1713-cd147-colorimetric-cell-based-elisa-wb-testing-1.jpgCD147 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/grk3-colorimetric-cell-based-elisa-ekc1714-boster.html2026-03-24T05:24:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRK3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1714-grk3-colorimetric-cell-based-elisa-wb-testing-1.jpgGRK3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sox6-colorimetric-cell-based-elisa-ekc1715-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSOX6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1715-sox6-colorimetric-cell-based-elisa-wb-testing-1.jpgSOX6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/acv1b-colorimetric-cell-based-elisa-ekc1716-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngACV1B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1716-acv1b-colorimetric-cell-based-elisa-wb-testing-1.jpgACV1B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/mouse liver cells
https://www.bosterbio.com/products/cell-based-elisa-kits/acvl1-colorimetric-cell-based-elisa-ekc1717-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngACVL1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1717-acvl1-colorimetric-cell-based-elisa-wb-testing-1.jpgACVL1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/etv6-colorimetric-cell-based-elisa-ekc1718-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngETV6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1718-etv6-colorimetric-cell-based-elisa-wb-testing-1.jpgETV6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/bud31-colorimetric-cell-based-elisa-ekc1719-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBUD31 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1719-bud31-colorimetric-cell-based-elisa-wb-testing-1.jpgBUD31 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hnf4-alpha-gamma-colorimetric-cell-based-elisa-ekc1720-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHNF4 alpha/gamma Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1720-hnf4-alpha-gamma-colorimetric-cell-based-elisa-wb-testing-1.jpgHNF4 alpha/gamma Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/HeLa/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnfsf9-colorimetric-cell-based-elisa-ekc1721-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNFSF9 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1721-tnfsf9-colorimetric-cell-based-elisa-wb-testing-1.jpgTNFSF9 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/frk-colorimetric-cell-based-elisa-ekc1722-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFRK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1722-frk-colorimetric-cell-based-elisa-wb-testing-1.jpgFRK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cbx5-colorimetric-cell-based-elisa-ekc1723-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBX5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1723-cbx5-colorimetric-cell-based-elisa-wb-testing-1.jpgCBX5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/A549/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/id4-colorimetric-cell-based-elisa-ekc1725-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngID4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1725-id4-colorimetric-cell-based-elisa-wb-testing-1.jpgID4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tisd-colorimetric-cell-based-elisa-ekc1726-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTISD Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1726-tisd-colorimetric-cell-based-elisa-wb-testing-1.jpgTISD Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cki-alpha-colorimetric-cell-based-elisa-ekc1727-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKI-alpha Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1727-cki-alpha-colorimetric-cell-based-elisa-wb-testing-1.jpgCKI-alpha Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hoxa1-colorimetric-cell-based-elisa-ekc1728-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHOXA1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1728-hoxa1-colorimetric-cell-based-elisa-wb-testing-1.jpgHOXA1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/COLO/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cki-epsilon-colorimetric-cell-based-elisa-ekc1729-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKI-epsilon Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1729-cki-epsilon-colorimetric-cell-based-elisa-wb-testing-1.jpgCKI-epsilon Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cebpd-e-colorimetric-cell-based-elisa-ekc1730-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCEBPD/E Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1730-cebpd-e-colorimetric-cell-based-elisa-wb-testing-1.jpgCEBPD/E Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO/RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/clk2-colorimetric-cell-based-elisa-ekc1731-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCLK2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1731-clk2-colorimetric-cell-based-elisa-wb-testing-1.jpgCLK2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hint1-colorimetric-cell-based-elisa-ekc1732-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHINT1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1732-hint1-colorimetric-cell-based-elisa-wb-testing-1.jpgHINT1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/meox2-colorimetric-cell-based-elisa-ekc1733-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMEOX2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1733-meox2-colorimetric-cell-based-elisa-wb-testing-1.jpgMEOX2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/er81-colorimetric-cell-based-elisa-ekc1734-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngER81 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1734-er81-colorimetric-cell-based-elisa-wb-testing-1.jpgER81 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/galk1-colorimetric-cell-based-elisa-ekc1735-boster.html2026-03-24T05:24:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGALK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1735-galk1-colorimetric-cell-based-elisa-wb-testing-1.jpgGALK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/bmx-colorimetric-cell-based-elisa-ekc1736-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBMX Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1736-bmx-colorimetric-cell-based-elisa-wb-testing-1.jpgBMX Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mat1-colorimetric-cell-based-elisa-ekc1737-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAT1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1737-mat1-colorimetric-cell-based-elisa-wb-testing-1.jpgMAT1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hnrnp-m-colorimetric-cell-based-elisa-ekc1738-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pnghnRNP M Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1738-hnrnp-m-colorimetric-cell-based-elisa-wb-testing-1.jpghnRNP M Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ncbp2-colorimetric-cell-based-elisa-ekc1739-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNCBP2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1739-ncbp2-colorimetric-cell-based-elisa-wb-testing-1.jpgNCBP2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dgke-colorimetric-cell-based-elisa-ekc1740-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDGKE Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1740-dgke-colorimetric-cell-based-elisa-wb-testing-1.jpgDGKE Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/map2k6-colorimetric-cell-based-elisa-ekc1741-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP2K6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1741-map2k6-colorimetric-cell-based-elisa-wb-testing-1.jpgMAP2K6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tf2a1-colorimetric-cell-based-elisa-ekc1742-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTF2A1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1742-tf2a1-colorimetric-cell-based-elisa-wb-testing-1.jpgTF2A1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf134-colorimetric-cell-based-elisa-ekc1743-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF134 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1743-znf134-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF134 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/rbm5-colorimetric-cell-based-elisa-ekc1744-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRBM5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1744-rbm5-colorimetric-cell-based-elisa-wb-testing-1.jpgRBM5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hexokinase-3-colorimetric-cell-based-elisa-ekc1745-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHexokinase-3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1745-hexokinase-3-colorimetric-cell-based-elisa-wb-testing-1.jpgHexokinase-3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/efna3-colorimetric-cell-based-elisa-ekc1746-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEFNA3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1746-efna3-colorimetric-cell-based-elisa-wb-testing-1.jpgEFNA3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/efna5-colorimetric-cell-based-elisa-ekc1747-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEFNA5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1747-efna5-colorimetric-cell-based-elisa-wb-testing-1.jpgEFNA5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dgkq-colorimetric-cell-based-elisa-ekc1748-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDGKQ Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1748-dgkq-colorimetric-cell-based-elisa-wb-testing-1.jpgDGKQ Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/limk1-colorimetric-cell-based-elisa-ekc1749-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLIMK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1749-limk1-colorimetric-cell-based-elisa-wb-testing-1.jpgLIMK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pttg-colorimetric-cell-based-elisa-ekc1750-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPTTG Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1750-pttg-colorimetric-cell-based-elisa-wb-testing-1.jpgPTTG Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/prkag1-2-3-colorimetric-cell-based-elisa-ekc1751-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPRKAG1/2/3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1751-prkag1-2-3-colorimetric-cell-based-elisa-wb-testing-1.jpgPRKAG1/2/3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/af10-colorimetric-cell-based-elisa-ekc1752-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAF10 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1752-af10-colorimetric-cell-based-elisa-wb-testing-1.jpgAF10 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ell-colorimetric-cell-based-elisa-ekc1753-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngELL Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1753-ell-colorimetric-cell-based-elisa-wb-testing-1.jpgELL Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hnrnp-h-colorimetric-cell-based-elisa-ekc1754-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pnghnRNP H Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1754-hnrnp-h-colorimetric-cell-based-elisa-wb-testing-1.jpghnRNP H Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/293/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dlx5-colorimetric-cell-based-elisa-ekc1755-boster.html2026-03-24T05:24:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDLX5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1755-dlx5-colorimetric-cell-based-elisa-wb-testing-1.jpgDLX5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/293/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sox8-9-17-18-colorimetric-cell-based-elisa-ekc1756-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSOX8/9/17/18 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1756-sox8-9-17-18-colorimetric-cell-based-elisa-wb-testing-1.jpgSOX8/9/17/18 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tmprss3-colorimetric-cell-based-elisa-ekc1757-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTMPRSS3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1757-tmprss3-colorimetric-cell-based-elisa-wb-testing-1.jpgTMPRSS3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cks1-colorimetric-cell-based-elisa-ekc1758-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKS1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1758-cks1-colorimetric-cell-based-elisa-wb-testing-1.jpgCKS1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cops2-colorimetric-cell-based-elisa-ekc1759-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCOPS2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1759-cops2-colorimetric-cell-based-elisa-wb-testing-1.jpgCOPS2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pitx1-colorimetric-cell-based-elisa-ekc1760-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPITX1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1760-pitx1-colorimetric-cell-based-elisa-wb-testing-1.jpgPITX1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cki-gamma2-colorimetric-cell-based-elisa-ekc1761-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKI-gamma2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1761-cki-gamma2-colorimetric-cell-based-elisa-wb-testing-1.jpgCKI-gamma2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/293 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/st5-colorimetric-cell-based-elisa-ekc1763-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngST5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1763-st5-colorimetric-cell-based-elisa-wb-testing-1.jpgST5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adarb1-colorimetric-cell-based-elisa-ekc1764-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADARB1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1764-adarb1-colorimetric-cell-based-elisa-wb-testing-1.jpgADARB1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ssbp2-colorimetric-cell-based-elisa-ekc1765-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSSBP2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1765-ssbp2-colorimetric-cell-based-elisa-wb-testing-1.jpgSSBP2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/foxk1-colorimetric-cell-based-elisa-ekc1766-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1766-foxk1-colorimetric-cell-based-elisa-wb-testing-1.jpgFOXK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdkl1-colorimetric-cell-based-elisa-ekc1767-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDKL1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1767-cdkl1-colorimetric-cell-based-elisa-wb-testing-1.jpgCDKL1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/k6pp-colorimetric-cell-based-elisa-ekc1768-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngK6PP Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1768-k6pp-colorimetric-cell-based-elisa-wb-testing-1.jpgK6PP Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549/COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mef2b-colorimetric-cell-based-elisa-ekc1769-boster.html2026-03-24T05:24:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMEF2B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1769-mef2b-colorimetric-cell-based-elisa-wb-testing-1.jpgMEF2B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sp2-colorimetric-cell-based-elisa-ekc1770-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSP2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1770-sp2-colorimetric-cell-based-elisa-wb-testing-1.jpgSP2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/crem-colorimetric-cell-based-elisa-ekc1771-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCREM Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1771-crem-colorimetric-cell-based-elisa-wb-testing-1.jpgCREM Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mevalonate-kinase-colorimetric-cell-based-elisa-ekc1772-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMevalonate Kinase Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1772-mevalonate-kinase-colorimetric-cell-based-elisa-wb-testing-1.jpgMevalonate Kinase Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cebpz-colorimetric-cell-based-elisa-ekc1773-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCEBPZ Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1773-cebpz-colorimetric-cell-based-elisa-wb-testing-1.jpgCEBPZ Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hsf2-colorimetric-cell-based-elisa-ekc1774-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHSF2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1774-hsf2-colorimetric-cell-based-elisa-wb-testing-1.jpgHSF2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO/A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tle4-colorimetric-cell-based-elisa-ekc1775-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTLE4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1775-tle4-colorimetric-cell-based-elisa-wb-testing-1.jpgTLE4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/293/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mtssb-colorimetric-cell-based-elisa-ekc1776-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMtSSB Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1776-mtssb-colorimetric-cell-based-elisa-wb-testing-1.jpgMtSSB Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cstf1-colorimetric-cell-based-elisa-ekc1777-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCSTF1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1777-cstf1-colorimetric-cell-based-elisa-wb-testing-1.jpgCSTF1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sry-colorimetric-cell-based-elisa-ekc1778-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSRY Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1778-sry-colorimetric-cell-based-elisa-wb-testing-1.jpgSRY Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnfc-colorimetric-cell-based-elisa-ekc1779-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNFC Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1779-tnfc-colorimetric-cell-based-elisa-wb-testing-1.jpgTNFC Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sfrs4-colorimetric-cell-based-elisa-ekc1780-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSFRS4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1780-sfrs4-colorimetric-cell-based-elisa-wb-testing-1.jpgSFRS4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dmpk-colorimetric-cell-based-elisa-ekc1781-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDMPK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1781-dmpk-colorimetric-cell-based-elisa-wb-testing-1.jpgDMPK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ncbp1-colorimetric-cell-based-elisa-ekc1782-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNCBP1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1782-ncbp1-colorimetric-cell-based-elisa-wb-testing-1.jpgNCBP1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29/LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mn1-colorimetric-cell-based-elisa-ekc1783-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMN1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1783-mn1-colorimetric-cell-based-elisa-wb-testing-1.jpgMN1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tef-colorimetric-cell-based-elisa-ekc1784-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTEF Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1784-tef-colorimetric-cell-based-elisa-wb-testing-1.jpgTEF Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/rat brain cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akap13-colorimetric-cell-based-elisa-ekc1785-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP13 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1785-akap13-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP13 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nuclear-factor-1-colorimetric-cell-based-elisa-ekc1786-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNuclear Factor 1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1786-nuclear-factor-1-colorimetric-cell-based-elisa-wb-testing-1.jpgNuclear Factor 1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from rat brain/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/elavl2-colorimetric-cell-based-elisa-ekc1787-boster.html2026-03-24T05:24:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngELAVL2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1787-elavl2-colorimetric-cell-based-elisa-wb-testing-1.jpgELAVL2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/foxl1-colorimetric-cell-based-elisa-ekc1788-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXL1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1788-foxl1-colorimetric-cell-based-elisa-wb-testing-1.jpgFOXL1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7/A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dusp4-colorimetric-cell-based-elisa-ekc1789-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDUSP4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1789-dusp4-colorimetric-cell-based-elisa-wb-testing-1.jpgDUSP4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7/A549/COS7/NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/psmd2-colorimetric-cell-based-elisa-ekc1790-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPSMD2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1790-psmd2-colorimetric-cell-based-elisa-wb-testing-1.jpgPSMD2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tbx2-colorimetric-cell-based-elisa-ekc1791-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTBX2 Colorimetric Cell-Based ELISABoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/cgk-2-colorimetric-cell-based-elisa-ekc1792-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCGK 2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1792-cgk-2-colorimetric-cell-based-elisa-wb-testing-1.jpgCGK 2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdk5r2-colorimetric-cell-based-elisa-ekc1793-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK5R2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1793-cdk5r2-colorimetric-cell-based-elisa-wb-testing-1.jpgCDK5R2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mta1-colorimetric-cell-based-elisa-ekc1794-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMTA1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1794-mta1-colorimetric-cell-based-elisa-wb-testing-1.jpgMTA1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/jip2-colorimetric-cell-based-elisa-ekc1795-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngJIP2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1795-jip2-colorimetric-cell-based-elisa-wb-testing-1.jpgJIP2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse brain cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tcof1-colorimetric-cell-based-elisa-ekc1796-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTCOF1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1796-tcof1-colorimetric-cell-based-elisa-wb-testing-1.jpgTCOF1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/293/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tusc3-colorimetric-cell-based-elisa-ekc1797-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTUSC3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1797-tusc3-colorimetric-cell-based-elisa-wb-testing-1.jpgTUSC3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/HeLa cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/serc3-colorimetric-cell-based-elisa-ekc1800-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSERC3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1800-serc3-colorimetric-cell-based-elisa-wb-testing-1.jpgSERC3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/camk2-beta-gamma-colorimetric-cell-based-elisa-ekc1801-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaMK2 beta/gamma Colorimetric Cell-Based ELISABoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/itpk1-colorimetric-cell-based-elisa-ekc1802-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngITPK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1802-itpk1-colorimetric-cell-based-elisa-wb-testing-1.jpgITPK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tra-2-alpha-colorimetric-cell-based-elisa-ekc1803-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRA-2 alpha Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1803-tra-2-alpha-colorimetric-cell-based-elisa-wb-testing-1.jpgTRA-2 alpha Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dyr1a-colorimetric-cell-based-elisa-ekc1804-boster.html2026-03-24T05:24:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDYR1A Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1804-dyr1a-colorimetric-cell-based-elisa-wb-testing-1.jpgDYR1A Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tf2h2-colorimetric-cell-based-elisa-ekc1805-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTF2H2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1805-tf2h2-colorimetric-cell-based-elisa-wb-testing-1.jpgTF2H2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/git2-colorimetric-cell-based-elisa-ekc1806-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGIT2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1806-git2-colorimetric-cell-based-elisa-wb-testing-1.jpgGIT2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf147-colorimetric-cell-based-elisa-ekc1807-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF147 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1807-znf147-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF147 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/glucokinase-regulator-colorimetric-cell-based-elisa-ekc1808-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGlucokinase Regulator Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1808-glucokinase-regulator-colorimetric-cell-based-elisa-wb-testing-1.jpgGlucokinase Regulator Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse heart/rat heart cells
https://www.bosterbio.com/products/cell-based-elisa-kits/gk3-colorimetric-cell-based-elisa-ekc1809-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGK3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1809-gk3-colorimetric-cell-based-elisa-wb-testing-1.jpgGK3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/gk2-colorimetric-cell-based-elisa-ekc1810-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGK2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1810-gk2-colorimetric-cell-based-elisa-wb-testing-1.jpgGK2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/293/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/melk-colorimetric-cell-based-elisa-ekc1811-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMELK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1811-melk-colorimetric-cell-based-elisa-wb-testing-1.jpgMELK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf638-colorimetric-cell-based-elisa-ekc1812-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF638 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1812-znf638-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF638 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nr1i3-colorimetric-cell-based-elisa-ekc1813-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNR1I3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1813-nr1i3-colorimetric-cell-based-elisa-wb-testing-1.jpgNR1I3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fakd3-colorimetric-cell-based-elisa-ekc1814-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAKD3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1814-fakd3-colorimetric-cell-based-elisa-wb-testing-1.jpgFAKD3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/wtap-colorimetric-cell-based-elisa-ekc1815-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngWTAP Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1815-wtap-colorimetric-cell-based-elisa-wb-testing-1.jpgWTAP Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/brd3-colorimetric-cell-based-elisa-ekc1816-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBRD3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1816-brd3-colorimetric-cell-based-elisa-wb-testing-1.jpgBRD3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdk5r1-colorimetric-cell-based-elisa-ekc1817-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK5R1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1817-cdk5r1-colorimetric-cell-based-elisa-wb-testing-1.jpgCDK5R1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from rat brain cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdk10-colorimetric-cell-based-elisa-ekc1818-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK10 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1818-cdk10-colorimetric-cell-based-elisa-wb-testing-1.jpgCDK10 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/irf4-colorimetric-cell-based-elisa-ekc1819-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRF4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1819-irf4-colorimetric-cell-based-elisa-wb-testing-1.jpgIRF4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ankrd1-colorimetric-cell-based-elisa-ekc1820-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngANKRD1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1820-ankrd1-colorimetric-cell-based-elisa-wb-testing-1.jpgANKRD1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/surf1-colorimetric-cell-based-elisa-ekc1821-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSURF1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1821-surf1-colorimetric-cell-based-elisa-wb-testing-1.jpgSURF1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ssxt-colorimetric-cell-based-elisa-ekc1822-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSSXT Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1822-ssxt-colorimetric-cell-based-elisa-wb-testing-1.jpgSSXT Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/taf1a-colorimetric-cell-based-elisa-ekc1823-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTAF1A Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1823-taf1a-colorimetric-cell-based-elisa-wb-testing-1.jpgTAF1A Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tsn-colorimetric-cell-based-elisa-ekc1824-boster.html2026-03-24T05:24:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTSN Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1824-tsn-colorimetric-cell-based-elisa-wb-testing-1.jpgTSN Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/trip13-colorimetric-cell-based-elisa-ekc1825-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRIP13 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1825-trip13-colorimetric-cell-based-elisa-wb-testing-1.jpgTRIP13 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO/NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/med1-colorimetric-cell-based-elisa-ekc1826-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMED1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1826-med1-colorimetric-cell-based-elisa-wb-testing-1.jpgMED1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/rhoh-colorimetric-cell-based-elisa-ekc1827-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRhoH Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1827-rhoh-colorimetric-cell-based-elisa-wb-testing-1.jpgRhoH Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf174-colorimetric-cell-based-elisa-ekc1828-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF174 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1828-znf174-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF174 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tsc22d1-colorimetric-cell-based-elisa-ekc1829-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTSC22D1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1829-tsc22d1-colorimetric-cell-based-elisa-wb-testing-1.jpgTSC22D1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse liver/rat liver cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nab2-colorimetric-cell-based-elisa-ekc1830-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNAB2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1830-nab2-colorimetric-cell-based-elisa-wb-testing-1.jpgNAB2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29/RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ogr-1-colorimetric-cell-based-elisa-ekc1831-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngOGR-1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1831-ogr-1-colorimetric-cell-based-elisa-wb-testing-1.jpgOGR-1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ndf2-colorimetric-cell-based-elisa-ekc1832-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNDF2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1832-ndf2-colorimetric-cell-based-elisa-wb-testing-1.jpgNDF2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/COLO/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/smad1-5-9-colorimetric-cell-based-elisa-ekc1833-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSmad1/5/9 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1833-smad1-5-9-colorimetric-cell-based-elisa-wb-testing-1.jpgSmad1/5/9 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/vps72-colorimetric-cell-based-elisa-ekc1834-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVPS72 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1834-vps72-colorimetric-cell-based-elisa-wb-testing-1.jpgVPS72 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7/NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mapk3-colorimetric-cell-based-elisa-ekc1835-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAPK3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1835-mapk3-colorimetric-cell-based-elisa-wb-testing-1.jpgMAPK3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nfil3-colorimetric-cell-based-elisa-ekc1836-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNFIL3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1836-nfil3-colorimetric-cell-based-elisa-wb-testing-1.jpgNFIL3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdkn3-colorimetric-cell-based-elisa-ekc1837-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDKN3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1837-cdkn3-colorimetric-cell-based-elisa-wb-testing-1.jpgCDKN3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf187-colorimetric-cell-based-elisa-ekc1838-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF187 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1838-znf187-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF187 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dgkd-colorimetric-cell-based-elisa-ekc1839-boster.html2026-03-24T05:24:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDGKD Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1839-dgkd-colorimetric-cell-based-elisa-wb-testing-1.jpgDGKD Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pfkfb1-4-colorimetric-cell-based-elisa-ekc1840-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPFKFB1/4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1840-pfkfb1-4-colorimetric-cell-based-elisa-wb-testing-1.jpgPFKFB1/4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HepG2/COLO205/293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dok7-colorimetric-cell-based-elisa-ekc1841-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDOK7 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1841-dok7-colorimetric-cell-based-elisa-wb-testing-1.jpgDOK7 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from mouse brain cells
https://www.bosterbio.com/products/cell-based-elisa-kits/aak1-colorimetric-cell-based-elisa-ekc1842-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAAK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1842-aak1-colorimetric-cell-based-elisa-wb-testing-1.jpgAAK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dak-colorimetric-cell-based-elisa-ekc1843-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDAK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1843-dak-colorimetric-cell-based-elisa-wb-testing-1.jpgDAK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562/A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adck5-colorimetric-cell-based-elisa-ekc1844-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADCK5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1844-adck5-colorimetric-cell-based-elisa-wb-testing-1.jpgADCK5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/293/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cerkl-colorimetric-cell-based-elisa-ekc1845-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCERKL Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1845-cerkl-colorimetric-cell-based-elisa-wb-testing-1.jpgCERKL Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/plch-colorimetric-cell-based-elisa-ekc1846-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPLCH Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1846-plch-colorimetric-cell-based-elisa-wb-testing-1.jpgPLCH Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells/COLO205 cells/HeLa cells/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/qorx-colorimetric-cell-based-elisa-ekc1847-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngQORX Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1847-qorx-colorimetric-cell-based-elisa-wb-testing-1.jpgQORX Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fakd1-colorimetric-cell-based-elisa-ekc1848-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAKD1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1848-fakd1-colorimetric-cell-based-elisa-wb-testing-1.jpgFAKD1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sgol1-colorimetric-cell-based-elisa-ekc1849-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSGOL1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1849-sgol1-colorimetric-cell-based-elisa-wb-testing-1.jpgSGOL1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cdkl4-colorimetric-cell-based-elisa-ekc1850-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDKL4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1850-cdkl4-colorimetric-cell-based-elisa-wb-testing-1.jpgCDKL4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/rbm26-colorimetric-cell-based-elisa-ekc1851-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRBM26 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1851-rbm26-colorimetric-cell-based-elisa-wb-testing-1.jpgRBM26 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/map3kl4-colorimetric-cell-based-elisa-ekc1852-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP3KL4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1852-map3kl4-colorimetric-cell-based-elisa-wb-testing-1.jpgMAP3KL4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ttc23-colorimetric-cell-based-elisa-ekc1853-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTTC23 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1853-ttc23-colorimetric-cell-based-elisa-wb-testing-1.jpgTTC23 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stea3-colorimetric-cell-based-elisa-ekc1854-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTEA3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1854-stea3-colorimetric-cell-based-elisa-wb-testing-1.jpgSTEA3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ldoc1l-colorimetric-cell-based-elisa-ekc1855-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLDOC1L Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1855-ldoc1l-colorimetric-cell-based-elisa-wb-testing-1.jpgLDOC1L Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/camk1-beta-colorimetric-cell-based-elisa-ekc1856-boster.html2026-03-24T05:25:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaMK1-beta Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1856-camk1-beta-colorimetric-cell-based-elisa-wb-testing-1.jpgCaMK1-beta Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ksr2-colorimetric-cell-based-elisa-ekc1857-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKSR2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1857-ksr2-colorimetric-cell-based-elisa-wb-testing-1.jpgKSR2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mol2c-colorimetric-cell-based-elisa-ekc1858-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMOL2C Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1858-mol2c-colorimetric-cell-based-elisa-wb-testing-1.jpgMOL2C Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/armcx2-colorimetric-cell-based-elisa-ekc1859-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngARMCX2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1859-armcx2-colorimetric-cell-based-elisa-wb-testing-1.jpgARMCX2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/maea-colorimetric-cell-based-elisa-ekc1860-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAEA Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1860-maea-colorimetric-cell-based-elisa-wb-testing-1.jpgMAEA Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adck2-colorimetric-cell-based-elisa-ekc1861-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADCK2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1861-adck2-colorimetric-cell-based-elisa-wb-testing-1.jpgADCK2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mobkl2b-colorimetric-cell-based-elisa-ekc1862-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMOBKL2B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1862-mobkl2b-colorimetric-cell-based-elisa-wb-testing-1.jpgMOBKL2B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adck1-colorimetric-cell-based-elisa-ekc1863-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADCK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1863-adck1-colorimetric-cell-based-elisa-wb-testing-1.jpgADCK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549/RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akap14-colorimetric-cell-based-elisa-ekc1864-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP14 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1864-akap14-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP14 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/K562/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/hormad1-colorimetric-cell-based-elisa-ekc1865-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHORMAD1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1865-hormad1-colorimetric-cell-based-elisa-wb-testing-1.jpgHORMAD1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HuvEc/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/siah1-colorimetric-cell-based-elisa-ekc1866-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSIAH1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1866-siah1-colorimetric-cell-based-elisa-wb-testing-1.jpgSIAH1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/trim59-colorimetric-cell-based-elisa-ekc1867-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTRIM59 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1867-trim59-colorimetric-cell-based-elisa-wb-testing-1.jpgTRIM59 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lmtk2-colorimetric-cell-based-elisa-ekc1868-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLMTK2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1868-lmtk2-colorimetric-cell-based-elisa-wb-testing-1.jpgLMTK2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ccrk-colorimetric-cell-based-elisa-ekc1869-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCCRK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1869-ccrk-colorimetric-cell-based-elisa-wb-testing-1.jpgCCRK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tbc1d3-colorimetric-cell-based-elisa-ekc1870-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTBC1D3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1870-tbc1d3-colorimetric-cell-based-elisa-wb-testing-1.jpgTBC1D3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/map4k6-colorimetric-cell-based-elisa-ekc1871-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP4K6 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1871-map4k6-colorimetric-cell-based-elisa-wb-testing-1.jpgMAP4K6 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dclk2-colorimetric-cell-based-elisa-ekc1872-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDCLK2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1872-dclk2-colorimetric-cell-based-elisa-wb-testing-1.jpgDCLK2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/rgag1-colorimetric-cell-based-elisa-ekc1873-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRGAG1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1873-rgag1-colorimetric-cell-based-elisa-wb-testing-1.jpgRGAG1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf397-colorimetric-cell-based-elisa-ekc1874-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF397 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1874-znf397-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF397 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ankk1-colorimetric-cell-based-elisa-ekc1875-boster.html2026-03-24T05:25:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngANKK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1875-ankk1-colorimetric-cell-based-elisa-wb-testing-1.jpgANKK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ipmk-colorimetric-cell-based-elisa-ekc1876-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIPMK Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1876-ipmk-colorimetric-cell-based-elisa-wb-testing-1.jpgIPMK Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29/COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ppp1r14c-colorimetric-cell-based-elisa-ekc1877-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPPP1R14C Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1877-ppp1r14c-colorimetric-cell-based-elisa-wb-testing-1.jpgPPP1R14C Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lmo3-colorimetric-cell-based-elisa-ekc1878-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLMO3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1878-lmo3-colorimetric-cell-based-elisa-wb-testing-1.jpgLMO3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/smrc2-colorimetric-cell-based-elisa-ekc1879-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSMRC2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1879-smrc2-colorimetric-cell-based-elisa-wb-testing-1.jpgSMRC2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf596-colorimetric-cell-based-elisa-ekc1880-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF596 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1880-znf596-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF596 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/brsk1-colorimetric-cell-based-elisa-ekc1881-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBRSK1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1881-brsk1-colorimetric-cell-based-elisa-wb-testing-1.jpgBRSK1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/es8l1-colorimetric-cell-based-elisa-ekc1882-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngES8L1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1882-es8l1-colorimetric-cell-based-elisa-wb-testing-1.jpgES8L1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/grk7-colorimetric-cell-based-elisa-ekc1883-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRK7 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1883-grk7-colorimetric-cell-based-elisa-wb-testing-1.jpgGRK7 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO205/Jurkat/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tf3c2-colorimetric-cell-based-elisa-ekc1884-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTF3C2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1884-tf3c2-colorimetric-cell-based-elisa-wb-testing-1.jpgTF3C2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2/COLO205/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf785-colorimetric-cell-based-elisa-ekc1885-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF785 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1885-znf785-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF785 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/madd-colorimetric-cell-based-elisa-ekc1886-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMADD Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1886-madd-colorimetric-cell-based-elisa-wb-testing-1.jpgMADD Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO/HeLa/Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/sept1-colorimetric-cell-based-elisa-ekc1887-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSEPT1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1887-sept1-colorimetric-cell-based-elisa-wb-testing-1.jpgSEPT1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/smg7-colorimetric-cell-based-elisa-ekc1888-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSMG7 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1888-smg7-colorimetric-cell-based-elisa-wb-testing-1.jpgSMG7 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat/293/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akap1-colorimetric-cell-based-elisa-ekc1889-boster.html2026-03-24T05:25:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKAP1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1889-akap1-colorimetric-cell-based-elisa-wb-testing-1.jpgAKAP1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/requ-colorimetric-cell-based-elisa-ekc1890-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngREQU Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1890-requ-colorimetric-cell-based-elisa-wb-testing-1.jpgREQU Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cops5-colorimetric-cell-based-elisa-ekc1891-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCOPS5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1891-cops5-colorimetric-cell-based-elisa-wb-testing-1.jpgCOPS5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293/K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/foxj1-colorimetric-cell-based-elisa-ekc1892-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXJ1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1892-foxj1-colorimetric-cell-based-elisa-wb-testing-1.jpgFOXJ1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dlx4-colorimetric-cell-based-elisa-ekc1893-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDLX4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1893-dlx4-colorimetric-cell-based-elisa-wb-testing-1.jpgDLX4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tf3b-colorimetric-cell-based-elisa-ekc1894-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTF3B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1894-tf3b-colorimetric-cell-based-elisa-wb-testing-1.jpgTF3B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Jurkat cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/isl2-colorimetric-cell-based-elisa-ekc1896-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngISL2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1896-isl2-colorimetric-cell-based-elisa-wb-testing-1.jpgISL2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adck4-colorimetric-cell-based-elisa-ekc1897-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADCK4 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1897-adck4-colorimetric-cell-based-elisa-wb-testing-1.jpgADCK4 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ip3kc-colorimetric-cell-based-elisa-ekc1898-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIP3KC Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1898-ip3kc-colorimetric-cell-based-elisa-wb-testing-1.jpgIP3KC Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/mastl-colorimetric-cell-based-elisa-ekc1899-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMASTL Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1899-mastl-colorimetric-cell-based-elisa-wb-testing-1.jpgMASTL Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pgbd1-colorimetric-cell-based-elisa-ekc1900-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPGBD1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1900-pgbd1-colorimetric-cell-based-elisa-wb-testing-1.jpgPGBD1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/znf668-colorimetric-cell-based-elisa-ekc1901-boster.html2026-03-24T05:25:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZNF668 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1901-znf668-colorimetric-cell-based-elisa-wb-testing-1.jpgZNF668 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fam84b-colorimetric-cell-based-elisa-ekc1902-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAM84B Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1902-fam84b-colorimetric-cell-based-elisa-wb-testing-1.jpgFAM84B Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/rps6kc1-colorimetric-cell-based-elisa-ekc1903-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRPS6KC1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1903-rps6kc1-colorimetric-cell-based-elisa-wb-testing-1.jpgRPS6KC1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/foxb1-2-colorimetric-cell-based-elisa-ekc1904-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXB1/2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1904-foxb1-2-colorimetric-cell-based-elisa-wb-testing-1.jpgFOXB1/2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-17a1-colorimetric-cell-based-elisa-ekc1905-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 17A1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1905-cytochrome-p450-17a1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 17A1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat/A549/HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-21a2-colorimetric-cell-based-elisa-ekc1906-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 21A2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1906-cytochrome-p450-21a2-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 21A2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-24a1-colorimetric-cell-based-elisa-ekc1907-boster.html2026-03-24T05:25:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 24A1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1907-cytochrome-p450-24a1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 24A1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from JurKat cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-2s1-colorimetric-cell-based-elisa-ekc1916-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 2S1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1916-cytochrome-p450-2s1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 2S1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HuvEc/COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-2u1-colorimetric-cell-based-elisa-ekc1917-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 2U1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1917-cytochrome-p450-2u1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 2U1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa/Lovo cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-39a1-colorimetric-cell-based-elisa-ekc1919-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 39A1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1919-cytochrome-p450-39a1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 39A1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO/HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-3a4-5-colorimetric-cell-based-elisa-ekc1920-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 3A4/5 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1920-cytochrome-p450-3a4-5-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 3A4/5 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-4b1-colorimetric-cell-based-elisa-ekc1921-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 4B1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1921-cytochrome-p450-4b1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 4B1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from COLO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-7b1-colorimetric-cell-based-elisa-ekc1922-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 7B1 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1922-cytochrome-p450-7b1-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 7B1 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cytochrome-p450-3a7-colorimetric-cell-based-elisa-ekc1923-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCytochrome P450 3A7 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1923-cytochrome-p450-3a7-colorimetric-cell-based-elisa-wb-testing-1.jpgCytochrome P450 3A7 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from LOVO cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cbp-colorimetric-cell-based-elisa-ekc1924-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBP Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1924-cbp-colorimetric-cell-based-elisa-wb-testing-1.jpgCBP Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HT-29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/adrb2-colorimetric-cell-based-elisa-ekc1925-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADRB2 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1925-adrb2-colorimetric-cell-based-elisa-wb-testing-1.jpgADRB2 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/afx-colorimetric-cell-based-elisa-ekc1926-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAFX Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1926-afx-colorimetric-cell-based-elisa-wb-testing-1.jpgAFX Colorimetric Cell-Based ELISAWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-2-3-colorimetric-cell-based-elisa-ekc1927-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAKT1/2/3 Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1927-akt1-2-3-colorimetric-cell-based-elisa-wb-testing-1.jpgAKT1/2/3 Colorimetric Cell-Based ELISAWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/atpase-colorimetric-cell-based-elisa-ekc1928-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATPase Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1928-atpase-colorimetric-cell-based-elisa-wb-testing-1.jpgATPase Colorimetric Cell-Based ELISAWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/enos-colorimetric-cell-based-elisa-ekc1929-boster.html2026-03-24T05:25:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngeNOS Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1929-enos-colorimetric-cell-based-elisa-ekc1929-wb-testing-1.jpgeNOS Colorimetric Cell-Based ELISAWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fkhr-colorimetric-cell-based-elisa-ekc1930-boster.html2026-03-24T05:25:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFKHR Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1930-fkhr-colorimetric-cell-based-elisa-wb-testing-1.jpgFKHR Colorimetric Cell-Based ELISAWestern blot analysis of extracts from HeLa cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/synuclein-pan-colorimetric-cell-based-elisa-ekc1932-boster.html2026-03-24T05:25:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSynuclein-pan Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1932-synuclein-pan-colorimetric-cell-based-elisa-wb-testing-1.jpgSynuclein-pan Colorimetric Cell-Based ELISAWestern blot analysis of extracts from rat heart cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/add1-2-phospho-ser726-colorimetric-cell-based-elisa-kit-ekc1935-boster.html2026-03-24T05:25:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngADD1/2 (Phospho-Ser726) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1935-add1-2-phospho-ser726-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgADD1/2 (Phospho-Ser726) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Forskolin 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/ampkalpha-phospho-thr172-colorimetric-cell-based-elisa-kit-ekc1936-boster.html2026-03-24T05:25:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAMPKalpha (Phospho-Thr172) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1936-ampkalpha-phospho-thr172-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAMPKalpha (Phospho-Thr172) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/amyloid-beta-a4-phospho-thr743-668-colorimetric-cell-based-elisa-kit-ekc1937-boster.html2026-03-24T05:25:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAmyloid beta A4 (Phospho-Thr743/668) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1937-amyloid-beta-a4-phospho-thr743-668-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAmyloid beta A4 (Phospho-Thr743/668) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/darpp-32-phospho-thr75-colorimetric-cell-based-elisa-kit-ekc1938-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1938-darpp-32-phospho-thr75-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDARPP-32 (Phospho-Thr75) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-thr678-colorimetric-cell-based-elisa-kit-ekc1939-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Thr678) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1939-egfr-phospho-thr678-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Thr678) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-thr693-colorimetric-cell-based-elisa-kit-ekc1940-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Thr693) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1940-egfr-phospho-thr693-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Thr693) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A431 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ephrin-b1-b2-phospho-tyr330-colorimetric-cell-based-elisa-kit-ekc1941-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEphrin B1/B2 (Phospho-Tyr330) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1941-ephrin-b1-b2-phospho-tyr330-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEphrin B1/B2 (Phospho-Tyr330) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with TNF-a 20ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/hsp90b-phospho-ser254-colorimetric-cell-based-elisa-kit-ekc1943-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1943-hsp90b-phospho-ser254-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHSP90B (Phospho-Ser254) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/keratin-18-phospho-ser33-colorimetric-cell-based-elisa-kit-ekc1946-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKeratin 18 (Phospho-Ser33) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1946-keratin-18-phospho-ser33-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgKeratin 18 (Phospho-Ser33) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/mef2a-phospho-ser408-colorimetric-cell-based-elisa-kit-ekc1949-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMEF2A (Phospho-Ser408) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1949-mef2a-phospho-ser408-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMEF2A (Phospho-Ser408) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/myc-phospho-ser62-colorimetric-cell-based-elisa-kit-ekc1950-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMyc (Phospho-Ser62) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1950-myc-phospho-ser62-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMyc (Phospho-Ser62) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Forskolin 40nM 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/shc-phospho-tyr349-colorimetric-cell-based-elisa-kit-ekc1953-boster.html2026-03-24T05:25:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngShc (Phospho-Tyr349) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1953-shc-phospho-tyr349-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgShc (Phospho-Tyr349) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/plcg1-phospho-tyr783-colorimetric-cell-based-elisa-kit-ekc1994-boster.html2026-03-24T05:25:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPLCG1 (Phospho-Tyr783) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1994-plcg1-phospho-tyr783-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPLCG1 (Phospho-Tyr783) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/polr2a-phospho-ser1619-colorimetric-cell-based-elisa-kit-ekc1996-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPOLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1996-polr2a-phospho-ser1619-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPOLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/rad17-phospho-ser645-colorimetric-cell-based-elisa-kit-ekc1999-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRAD17 (Phospho-Ser645) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc1999-rad17-phospho-ser645-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRAD17 (Phospho-Ser645) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/separase-phospho-ser801-colorimetric-cell-based-elisa-kit-ekc2000-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2000-separase-phospho-ser801-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSEPARASE (Phospho-Ser801) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/sgk-phospho-ser422-colorimetric-cell-based-elisa-kit-ekc2001-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSGK (Phospho-Ser422) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2001-sgk-phospho-ser422-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSGK (Phospho-Ser422) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/srf-phospho-ser99-colorimetric-cell-based-elisa-kit-ekc2002-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSRF (Phospho-Ser99) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2002-srf-phospho-ser99-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSRF (Phospho-Ser99) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from LOVO cells treated with Serum 10% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/stat2-phospho-tyr690-colorimetric-cell-based-elisa-kit-ekc2003-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT2 (Phospho-Tyr690) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2003-stat2-phospho-tyr690-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT2 (Phospho-Tyr690) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with IFN 2500U/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/syk-phospho-tyr348-colorimetric-cell-based-elisa-kit-ekc2005-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSYK (Phospho-Tyr348) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2005-syk-phospho-tyr348-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSYK (Phospho-Tyr348) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/syk-phospho-tyr525-colorimetric-cell-based-elisa-kit-ekc2006-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSYK (Phospho-Tyr525) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2006-syk-phospho-tyr525-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSYK (Phospho-Tyr525) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/wnk1-phospho-thr58-colorimetric-cell-based-elisa-kit-ekc2007-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngWNK1 (Phospho-Thr58) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2007-wnk1-phospho-thr58-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgWNK1 (Phospho-Thr58) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/vasp-phospho-ser157-colorimetric-cell-based-elisa-kit-ekc2008-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVASP (Phospho-Ser157) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2008-vasp-phospho-ser157-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVASP (Phospho-Ser157) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with forskolin 40 muM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/vav1-phospho-tyr174-colorimetric-cell-based-elisa-kit-ekc2009-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVAV1 (Phospho-Tyr174) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2009-vav1-phospho-tyr174-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVAV1 (Phospho-Tyr174) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/irs-1-phospho-ser307-colorimetric-cell-based-elisa-kit-ekc2010-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRS-1 (Phospho-Ser307) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2010-irs-1-phospho-ser307-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRS-1 (Phospho-Ser307) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nf-kappab-p65-phospho-ser529-colorimetric-cell-based-elisa-kit-ekc2011-boster.html2026-03-24T05:25:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNF-kappaB p65 (Phospho-Ser529) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2011-nf-kappab-p65-phospho-ser529-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNF-kappaB p65 (Phospho-Ser529) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa and Jurkat cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/atf1-phospho-ser63-colorimetric-cell-based-elisa-kit-ekc2019-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATF1 (Phospho-Ser63) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2019-atf1-phospho-ser63-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgATF1 (Phospho-Ser63) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with Insulin 0.01U/ML 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/csk-phospho-ser364-colorimetric-cell-based-elisa-kit-ekc2023-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCsk (Phospho-Ser364) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2023-csk-phospho-ser364-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCsk (Phospho-Ser364) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/cyclin-d3-phospho-thr283-colorimetric-cell-based-elisa-kit-ekc2025-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCyclin D3 (Phospho-Thr283) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2025-cyclin-d3-phospho-thr283-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCyclin D3 (Phospho-Thr283) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with UV 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/daxx-phospho-ser668-colorimetric-cell-based-elisa-kit-ekc2026-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDaxx (Phospho-Ser668) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2026-daxx-phospho-ser668-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDaxx (Phospho-Ser668) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PBS 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/opioid-receptor-delta-phospho-ser363-colorimetric-cell-based-elisa-kit-ekc2027-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngOpioid Receptor-delta (Phospho-Ser363) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2027-opioid-receptor-delta-phospho-ser363-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgOpioid Receptor-delta (Phospho-Ser363) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with TSA 400nM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/dynamin-1-phospho-ser774-colorimetric-cell-based-elisa-kit-ekc2028-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDynamin-1 (Phospho-Ser774) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2028-dynamin-1-phospho-ser774-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDynamin-1 (Phospho-Ser774) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/e2f1-phospho-thr433-colorimetric-cell-based-elisa-kit-ekc2029-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngE2F1 (Phospho-Thr433) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2029-e2f1-phospho-thr433-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgE2F1 (Phospho-Thr433) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Etoposide 25uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/fadd-phospho-ser194-colorimetric-cell-based-elisa-kit-ekc2030-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFADD (Phospho-Ser194) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2030-fadd-phospho-ser194-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFADD (Phospho-Ser194) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Paclitaxel 1uM 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-tyr576-colorimetric-cell-based-elisa-kit-ekc2031-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Tyr576) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2031-fak-phospho-tyr576-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Tyr576) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fancd2-phospho-ser222-colorimetric-cell-based-elisa-kit-ekc2032-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFANCD2 (Phospho-Ser222) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2032-fancd2-phospho-ser222-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFANCD2 (Phospho-Ser222) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with Calyculin A 50ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fos-phospho-ser362-colorimetric-cell-based-elisa-kit-ekc2033-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFos (Phospho-Ser362) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2033-fos-phospho-ser362-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFos (Phospho-Ser362) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with forskolin 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fyn-phospho-tyr530-colorimetric-cell-based-elisa-kit-ekc2034-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFyn (Phospho-Tyr530) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2034-fyn-phospho-tyr530-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFyn (Phospho-Tyr530) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with H2O2 100uM 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/glycogen-synthase-phospho-ser645-colorimetric-cell-based-elisa-kit-ekc2035-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGlycogen Synthase (Phospho-Ser645) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2035-glycogen-synthase-phospho-ser645-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgGlycogen Synthase (Phospho-Ser645) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/gr-phospho-ser226-colorimetric-cell-based-elisa-kit-ekc2036-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGR (Phospho-Ser226) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2036-gr-phospho-ser226-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgGR (Phospho-Ser226) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/histone-h3-phospho-thr3-colorimetric-cell-based-elisa-kit-ekc2037-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHistone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2037-histone-h3-phospho-thr3-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHistone H3 (Phospho-Thr3) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with Serum 20% 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/h3-phospho-ser28-colorhistone-imetric-cell-based-elisa-kit-ekc2038-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHistone H3 (Phospho-Ser28) ColorHistone imetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2038-h3-phospho-ser28-colorhistone-imetric-cell-based-elisa-kit-wb-testing-1.jpgHistone H3 (Phospho-Ser28) ColorHistone imetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with Serum 20% 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/hdac5-phospho-ser259-colorimetric-cell-based-elisa-kit-ekc2039-boster.html2026-03-24T05:25:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHDAC5 (Phospho-Ser259) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/hsl-phospho-ser552-colorimetric-cell-based-elisa-kit-ekc2040-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHSL (Phospho-Ser552) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2040-hsl-phospho-ser552-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHSL (Phospho-Ser552) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/ikk-alpha-beta-phospho-ser180-181-colorimetric-cell-based-elisa-kit-ekc2042-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIKK-alpha/beta (Phospho-Ser180/181) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2042-ikk-alpha-beta-phospho-ser180-181-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIKK-alpha/beta (Phospho-Ser180/181) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with TNF 20ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/ikk-alpha-phospho-ser176-ikk-beta-phospho-ser177-colorimetric-cell-based-elisa-kit-ekc2043-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2043-ikk-alpha-phospho-ser176-ikk-beta-phospho-ser177-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/ikk-beta-phospho-tyr188-colorimetric-cell-based-elisa-kit-ekc2044-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIKK-beta (Phospho-Tyr188) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2044-ikk-beta-phospho-tyr188-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIKK-beta (Phospho-Tyr188) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ikk-gamma-phospho-ser31-colorimetric-cell-based-elisa-kit-ekc2045-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIKK-gamma (Phospho-Ser31) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2045-ikk-gamma-phospho-ser31-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIKK-gamma (Phospho-Ser31) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with TNF-a 20ng/ml 5'
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https://www.bosterbio.com/products/cell-based-elisa-kits/irs-1-phospho-ser1101-colorimetric-cell-based-elisa-kit-ekc2048-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRS-1 (Phospho-Ser1101) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2048-irs-1-phospho-ser1101-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRS-1 (Phospho-Ser1101) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with Calyculin A 50ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/irs-1-phospho-ser612-colorimetric-cell-based-elisa-kit-ekc2049-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRS-1 (Phospho-Ser612) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2049-irs-1-phospho-ser612-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRS-1 (Phospho-Ser612) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with insulin 0.01U/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/ku80-xrcc5-phospho-thr714-colorimetric-cell-based-elisa-kit-ekc2051-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKu80/XRCC5 (Phospho-Thr714) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2051-ku80-xrcc5-phospho-thr714-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgKu80/XRCC5 (Phospho-Thr714) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-s1-phospho-thr246-colorimetric-cell-based-elisa-kit-ekc2052-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 S1 (Phospho-Thr246) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2052-akt1-s1-phospho-thr246-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 S1 (Phospho-Thr246) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PDGF 50ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/arrestin-1-phospho-ser412-colorimetric-cell-based-elisa-kit-ekc2053-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngArrestin 1 (Phospho-Ser412) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2053-arrestin-1-phospho-ser412-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgArrestin 1 (Phospho-Ser412) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with Etoposide 25uM 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/atp1a1-phospho-ser16-colorimetric-cell-based-elisa-kit-ekc2054-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATP1A1 (Phospho-Ser16) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2054-atp1a1-phospho-ser16-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgATP1A1 (Phospho-Ser16) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/aura-phospho-thr288-colorimetric-cell-based-elisa-kit-ekc2055-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAurA (Phospho-Thr288) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2055-aura-phospho-thr288-colorimetric-cell-based-elisa-kit-ekc2055-wb-testing-1.jpgAurA (Phospho-Thr288) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/bcl2-phospho-ser70-colorimetric-cell-based-elisa-kit-ekc2056-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBCL2 (Phospho-Ser70) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/caldesmon-phospho-ser789-colorimetric-cell-based-elisa-kit-ekc2057-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaldesmon (Phospho-Ser789) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2057-caldesmon-phospho-ser789-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaldesmon (Phospho-Ser789) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/calnexin-phospho-ser583-colorimetric-cell-based-elisa-kit-ekc2058-boster.html2026-03-24T05:25:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCalnexin (Phospho-Ser583) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2058-calnexin-phospho-ser583-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCalnexin (Phospho-Ser583) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/cbl-phospho-tyr774-colorimetric-cell-based-elisa-kit-ekc2059-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBL (Phospho-Tyr774) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2059-cbl-phospho-tyr774-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCBL (Phospho-Tyr774) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/cbl-phospho-tyr700-colorimetric-cell-based-elisa-kit-ekc2060-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBL (Phospho-Tyr700) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2060-cbl-phospho-tyr700-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCBL (Phospho-Tyr700) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Na3VO4 0.3nM
https://www.bosterbio.com/products/cell-based-elisa-kits/cbl-phospho-tyr674-colorimetric-cell-based-elisa-kit-ekc2061-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCBL (Phospho-Tyr674) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2061-cbl-phospho-tyr674-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCBL (Phospho-Tyr674) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Na2VO3 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/cd3-zeta-phospho-tyr142-colorimetric-cell-based-elisa-kit-ekc2062-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD3 zeta (Phospho-Tyr142) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2062-cd3-zeta-phospho-tyr142-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCD3 zeta (Phospho-Tyr142) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/cdk1-cdc2-phospho-thr14-colorimetric-cell-based-elisa-kit-ekc2063-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK1/CDC2 (Phospho-Thr14) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2063-cdk1-cdc2-phospho-thr14-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDK1/CDC2 (Phospho-Thr14) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Forskolin 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/creb-phospho-ser121-colorimetric-cell-based-elisa-kit-ekc2064-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCREB (Phospho-Ser121) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2064-creb-phospho-ser121-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCREB (Phospho-Ser121) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Etoposide 25uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-tyr1016-colorimetric-cell-based-elisa-kit-ekc2065-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Tyr1016) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2065-egfr-phospho-tyr1016-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Tyr1016) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with Serum 20% 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/estrogen-receptor-alpha-phospho-tyr537-colorimetric-cell-based-elisa-kit-ekc2066-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEstrogen Receptor-alpha (Phospho-Tyr537) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2066-estrogen-receptor-alpha-phospho-tyr537-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEstrogen Receptor-alpha (Phospho-Tyr537) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ets1-phospho-thr38-colorimetric-cell-based-elisa-kit-ekc2067-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngETS1 (Phospho-Thr38) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2067-ets1-phospho-thr38-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgETS1 (Phospho-Thr38) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-tyr407-colorimetric-cell-based-elisa-kit-ekc2068-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Tyr407) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2068-fak-phospho-tyr407-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Tyr407) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fgfr1-phospho-tyr766-colorimetric-cell-based-elisa-kit-ekc2069-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFGFR1 (Phospho-Tyr766) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2069-fgfr1-phospho-tyr766-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFGFR1 (Phospho-Tyr766) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fgfr1-phospho-tyr654-colorimetric-cell-based-elisa-kit-ekc2070-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2070-fgfr1-phospho-tyr654-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFGFR1 (Phospho-Tyr654) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/flt3-phospho-tyr599-colorimetric-cell-based-elisa-kit-ekc2071-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFLT3 (Phospho-Tyr599) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2071-flt3-phospho-tyr599-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFLT3 (Phospho-Tyr599) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/foxo1a-phospho-ser329-colorimetric-cell-based-elisa-kit-ekc2072-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXO1A (Phospho-Ser329) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2072-foxo1a-phospho-ser329-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFOXO1A (Phospho-Ser329) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/grk2-phospho-ser29-colorimetric-cell-based-elisa-kit-ekc2073-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRK2 (Phospho-Ser29) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2073-grk2-phospho-ser29-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgGRK2 (Phospho-Ser29) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/ikk-beta-phospho-tyr199-colorimetric-cell-based-elisa-kit-ekc2074-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIKK-beta (Phospho-Tyr199) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2074-ikk-beta-phospho-tyr199-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIKK-beta (Phospho-Tyr199) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/integrin-beta1-phospho-thr789-colorimetric-cell-based-elisa-kit-ekc2075-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIntegrin beta1 (Phospho-Thr789) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2075-integrin-beta1-phospho-thr789-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIntegrin beta1 (Phospho-Thr789) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Ca2+ 40uM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/ir-phospho-tyr1355-colorimetric-cell-based-elisa-kit-ekc2076-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIR (Phospho-Tyr1355) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2076-ir-phospho-tyr1355-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIR (Phospho-Tyr1355) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Heat shock
https://www.bosterbio.com/products/cell-based-elisa-kits/ir-phospho-tyr1361-colorimetric-cell-based-elisa-kit-ekc2077-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIR (Phospho-Tyr1361) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2077-ir-phospho-tyr1361-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIR (Phospho-Tyr1361) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Heat shock
https://www.bosterbio.com/products/cell-based-elisa-kits/irf-3-phospho-ser385-colorimetric-cell-based-elisa-kit-ekc2078-boster.html2026-03-24T05:25:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRF-3 (Phospho-Ser385) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2078-irf-3-phospho-ser385-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRF-3 (Phospho-Ser385) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with INSULIN 0.01U/ML 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/irs-1-phospho-ser323-colorimetric-cell-based-elisa-kit-ekc2079-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRS-1 (Phospho-Ser323) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2079-irs-1-phospho-ser323-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRS-1 (Phospho-Ser323) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with Serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/jak2-phospho-tyr570-colorimetric-cell-based-elisa-kit-ekc2080-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngJAK2 (Phospho-Tyr570) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2080-jak2-phospho-tyr570-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgJAK2 (Phospho-Tyr570) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with etoposide 25uM 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/nfat3-phospho-ser676-colorimetric-cell-based-elisa-kit-ekc2091-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNFAT3 (Phospho-Ser676) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2091-nfat3-phospho-ser676-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNFAT3 (Phospho-Ser676) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Ca2+ 40uM 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/nmdar2b-phospho-tyr1474-colorimetric-cell-based-elisa-kit-ekc2093-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNMDAR2B (Phospho-Tyr1474) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2093-nmdar2b-phospho-tyr1474-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNMDAR2B (Phospho-Tyr1474) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/nnos-phospho-ser852-colorimetric-cell-based-elisa-kit-ekc2094-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngnNOS (Phospho-Ser852) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2094-nnos-phospho-ser852-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgnNOS (Phospho-Ser852) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/p53-phospho-ser20-colorimetric-cell-based-elisa-kit-ekc2095-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp53 (Phospho-Ser20) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2095-p53-phospho-ser20-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp53 (Phospho-Ser20) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/p70-s6-kinase-phospho-thr229-colorimetric-cell-based-elisa-kit-ekc2096-boster.html2026-03-24T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp70 S6 Kinase (Phospho-Thr229) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2096-p70-s6-kinase-phospho-thr229-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp70 S6 Kinase (Phospho-Thr229) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/pka-alpha-beta-cat-phospho-thr197-colorimetric-cell-based-elisa-kit-ekc2107-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKA alpha/beta CAT (Phospho-Thr197) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2107-pka-alpha-beta-cat-phospho-thr197-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKA alpha/beta CAT (Phospho-Thr197) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
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https://www.bosterbio.com/products/cell-based-elisa-kits/plcg2-phospho-tyr1217-colorimetric-cell-based-elisa-kit-ekc2109-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPLCG2 (Phospho-Tyr1217) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2109-plcg2-phospho-tyr1217-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPLCG2 (Phospho-Tyr1217) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pp2a-alpha-phospho-tyr307-colorimetric-cell-based-elisa-kit-ekc2110-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2110-pp2a-alpha-phospho-tyr307-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPP2A-alpha (Phospho-Tyr307) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ppar-gamma-phospho-ser112-colorimetric-cell-based-elisa-kit-ekc2111-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPPAR-gamma (Phospho-Ser112) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2111-ppar-gamma-phospho-ser112-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPPAR-gamma (Phospho-Ser112) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with Paclitaxel 1uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/progesterone-receptor-phospho-ser294-colorimetric-cell-based-elisa-kit-ekc2112-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngProgesterone Receptor (Phospho-Ser294) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2112-progesterone-receptor-phospho-ser294-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgProgesterone Receptor (Phospho-Ser294) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Etoposide 25uM 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/progesterone-receptor-phospho-ser400-colorimetric-cell-based-elisa-kit-ekc2113-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngProgesterone Receptor (Phospho-Ser400) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2113-progesterone-receptor-phospho-ser400-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgProgesterone Receptor (Phospho-Ser400) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with heat shock
https://www.bosterbio.com/products/cell-based-elisa-kits/rabphilin-3a-phospho-ser237-colorimetric-cell-based-elisa-kit-ekc2114-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabphilin 3A (Phospho-Ser237) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2114-rabphilin-3a-phospho-ser237-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRabphilin 3A (Phospho-Ser237) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells treated with Calyculin 100nM 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/c-raf-phospho-tyr341-colorimetric-cell-based-elisa-kit-ekc2115-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngC-RAF (Phospho-Tyr341) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2115-c-raf-phospho-tyr341-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgC-RAF (Phospho-Tyr341) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with Paclitaxel 1uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/c-raf-phospho-ser621-colorimetric-cell-based-elisa-kit-ekc2116-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngC-RAF (Phospho-Ser621) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2116-c-raf-phospho-ser621-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgC-RAF (Phospho-Ser621) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with UV 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/ret-phospho-tyr1015-colorimetric-cell-based-elisa-kit-ekc2117-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRet (Phospho-Tyr1015) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2117-ret-phospho-tyr1015-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRet (Phospho-Tyr1015) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/s6-ribosomal-protein-phospho-ser240-colorimetric-cell-based-elisa-kit-ekc2118-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngS6 Ribosomal Protein (Phospho-Ser240) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2118-s6-ribosomal-protein-phospho-ser240-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgS6 Ribosomal Protein (Phospho-Ser240) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml 2'
https://www.bosterbio.com/products/cell-based-elisa-kits/sf1-phospho-ser82-colorimetric-cell-based-elisa-kit-ekc2119-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSF1 (Phospho-Ser82) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2119-sf1-phospho-ser82-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSF1 (Phospho-Ser82) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with anisomycin 25ug/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/smc1-phospho-ser966-colorimetric-cell-based-elisa-kit-ekc2120-boster.html2026-03-24T05:25:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSMC1 (Phospho-Ser966) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2120-smc1-phospho-ser966-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSMC1 (Phospho-Ser966) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with etoposide 24uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/sox-9-phospho-ser181-colorimetric-cell-based-elisa-kit-ekc2121-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSOX-9 (Phospho-Ser181) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2121-sox-9-phospho-ser181-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSOX-9 (Phospho-Ser181) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PBS 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/sp1-phospho-thr453-colorimetric-cell-based-elisa-kit-ekc2122-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSP1 (Phospho-Thr453) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2122-sp1-phospho-thr453-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSP1 (Phospho-Thr453) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/survivin-phospho-thr34-colorimetric-cell-based-elisa-kit-ekc2123-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSurvivin (Phospho-Thr34) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2123-survivin-phospho-thr34-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSurvivin (Phospho-Thr34) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with EGF 100ug/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/syk-phospho-tyr323-colorimetric-cell-based-elisa-kit-ekc2124-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSYK (Phospho-Tyr323) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2124-syk-phospho-tyr323-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSYK (Phospho-Tyr323) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/synapsin1-phospho-ser62-colorimetric-cell-based-elisa-kit-ekc2125-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSynapsin1 (Phospho-Ser62) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2125-synapsin1-phospho-ser62-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSynapsin1 (Phospho-Ser62) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Anisomycin 25ug/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/tebp-phospho-ser113-colorimetric-cell-based-elisa-kit-ekc2127-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTEBP (Phospho-Ser113) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2127-tebp-phospho-ser113-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTEBP (Phospho-Ser113) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/tk-phospho-ser13-colorimetric-cell-based-elisa-kit-ekc2128-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTK (Phospho-Ser13) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2128-tk-phospho-ser13-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTK (Phospho-Ser13) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with paclitaxel 1uM 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/tnni3-phospho-thr142-colorimetric-cell-based-elisa-kit-ekc2130-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNNI3 (Phospho-Thr142) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2130-tnni3-phospho-thr142-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTNNI3 (Phospho-Thr142) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse heart
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https://www.bosterbio.com/products/cell-based-elisa-kits/vegfr2-phospho-tyr1059-colorimetric-cell-based-elisa-kit-ekc2133-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVEGFR2 (Phospho-Tyr1059) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2133-vegfr2-phospho-tyr1059-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVEGFR2 (Phospho-Tyr1059) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/wasp-phospho-tyr290-colorimetric-cell-based-elisa-kit-ekc2134-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngWASP (Phospho-Tyr290) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2134-wasp-phospho-tyr290-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgWASP (Phospho-Tyr290) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/wave1-phospho-tyr125-colorimetric-cell-based-elisa-kit-ekc2135-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngWAVE1 (Phospho-Tyr125) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2135-wave1-phospho-tyr125-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgWAVE1 (Phospho-Tyr125) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/zap-70-phospho-tyr292-colorimetric-cell-based-elisa-kit-ekc2136-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2136-zap-70-phospho-tyr292-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/zap-70-phospho-tyr315-colorimetric-cell-based-elisa-kit-ekc2137-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2137-zap-70-phospho-tyr315-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with Ca+ 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/ack1-phospho-tyr284-colorimetric-cell-based-elisa-kit-ekc2138-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngACK1 (Phospho-Tyr284) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2138-ack1-phospho-tyr284-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgACK1 (Phospho-Tyr284) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-phospho-tyr474-colorimetric-cell-based-elisa-kit-ekc2139-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 (Phospho-Tyr474) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2139-akt1-phospho-tyr474-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 (Phospho-Tyr474) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-phospho-ser246-colorimetric-cell-based-elisa-kit-ekc2140-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 (Phospho-Ser246) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2140-akt1-phospho-ser246-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 (Phospho-Ser246) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Etoposide 25uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-phospho-thr72-colorimetric-cell-based-elisa-kit-ekc2141-boster.html2026-03-24T05:25:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 (Phospho-Thr72) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2141-akt1-phospho-thr72-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 (Phospho-Thr72) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with TNF-a 20ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/alk-phospho-tyr1507-colorimetric-cell-based-elisa-kit-ekc2142-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngALK (Phospho-Tyr1507) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2142-alk-phospho-tyr1507-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgALK (Phospho-Tyr1507) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with anisomycin 25ug/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/alk-phospho-tyr1604-colorimetric-cell-based-elisa-kit-ekc2143-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngALK (Phospho-Tyr1604) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2143-alk-phospho-tyr1604-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgALK (Phospho-Tyr1604) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/atp-citrate-lyase-phospho-ser454-colorimetric-cell-based-elisa-kit-ekc2144-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATP-Citrate Lyase (Phospho-Ser454) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2144-atp-citrate-lyase-phospho-ser454-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgATP-Citrate Lyase (Phospho-Ser454) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with Calyculin 50nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/blnk-phospho-tyr96-colorimetric-cell-based-elisa-kit-ekc2145-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBLNK (Phospho-Tyr96) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2145-blnk-phospho-tyr96-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBLNK (Phospho-Tyr96) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/abl1-phospho-tyr245-colorimetric-cell-based-elisa-kit-ekc2146-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngABL1 (Phospho-Tyr245) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2146-abl1-phospho-tyr245-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgABL1 (Phospho-Tyr245) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Insulin 0.01U/ml 15
https://www.bosterbio.com/products/cell-based-elisa-kits/cd32-phospho-tyr292-colorimetric-cell-based-elisa-kit-ekc2147-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD32 (Phospho-Tyr292) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2147-cd32-phospho-tyr292-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCD32 (Phospho-Tyr292) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/cdc25c-phospho-thr48-colorimetric-cell-based-elisa-kit-ekc2148-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDC25C (Phospho-Thr48) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2148-cdc25c-phospho-thr48-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDC25C (Phospho-Thr48) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/chk1-phospho-ser296-colorimetric-cell-based-elisa-kit-ekc2149-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk1 (Phospho-Ser296) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2149-chk1-phospho-ser296-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk1 (Phospho-Ser296) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/c-raf-phospho-ser296-colorimetric-cell-based-elisa-kit-ekc2150-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngC-RAF (Phospho-Ser296) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2150-c-raf-phospho-ser296-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgC-RAF (Phospho-Ser296) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/eif4b-phospho-ser422-colorimetric-cell-based-elisa-kit-ekc2152-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngeIF4B (Phospho-Ser422) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2152-eif4b-phospho-ser422-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgeIF4B (Phospho-Ser422) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/etk-phospho-tyr40-colorimetric-cell-based-elisa-kit-ekc2153-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2153-etk-phospho-tyr40-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgETK (Phospho-Tyr40) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/irf-3-phospho-ser396-colorimetric-cell-based-elisa-kit-ekc2154-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRF-3 (Phospho-Ser396) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2154-irf-3-phospho-ser396-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRF-3 (Phospho-Ser396) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/mek1-phospho-ser298-colorimetric-cell-based-elisa-kit-ekc2160-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMEK1 (Phospho-Ser298) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2160-mek1-phospho-ser298-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMEK1 (Phospho-Ser298) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with PDGF 50ng/ml 20'
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https://www.bosterbio.com/products/cell-based-elisa-kits/myosin-regulatory-light-chain-2-phospho-ser18-colorimetric-cell-based-elisa-kit-ekc2164-boster.html2026-03-24T05:25:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMyosin regulatory light chain 2 (Phospho-Ser18) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2164-myosin-regulatory-light-chain-2-phospho-ser18-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMyosin regulatory light chain 2 (Phospho-Ser18) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from LOVO cells treated with H2O2 100uM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/nmdar1-phospho-ser896-colorimetric-cell-based-elisa-kit-ekc2165-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNMDAR1 (Phospho-Ser896) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2165-nmdar1-phospho-ser896-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNMDAR1 (Phospho-Ser896) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/npm-phospho-thr199-colorimetric-cell-based-elisa-kit-ekc2166-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNPM (Phospho-Thr199) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2166-npm-phospho-thr199-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNPM (Phospho-Thr199) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/bcar1-phospho-tyr165-colorimetric-cell-based-elisa-kit-ekc2167-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBCAR1 (Phospho-Tyr165) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2167-bcar1-phospho-tyr165-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBCAR1 (Phospho-Tyr165) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/bcar1-phospho-tyr249-colorimetric-cell-based-elisa-kit-ekc2168-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBCAR1 (Phospho-Tyr249) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2168-bcar1-phospho-tyr249-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBCAR1 (Phospho-Tyr249) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/p63-phospho-ser455-colorimetric-cell-based-elisa-kit-ekc2169-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp63 (Phospho-Ser455) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2169-p63-phospho-ser455-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp63 (Phospho-Ser455) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TNF 2500U/ML 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pak2-phospho-ser20-colorimetric-cell-based-elisa-kit-ekc2170-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPAK2 (Phospho-Ser20) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2170-pak2-phospho-ser20-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPAK2 (Phospho-Ser20) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Sorbitol 0.4M 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pbk-topk-phospho-thr9-colorimetric-cell-based-elisa-kit-ekc2171-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPBK/TOPK (Phospho-Thr9) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2171-pbk-topk-phospho-thr9-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPBK/TOPK (Phospho-Thr9) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with UV 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pea-15-phospho-ser104-colorimetric-cell-based-elisa-kit-ekc2172-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPEA-15 (Phospho-Ser104) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2172-pea-15-phospho-ser104-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPEA-15 (Phospho-Ser104) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with TNF 20ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/pim-1-phospho-tyr309-colorimetric-cell-based-elisa-kit-ekc2173-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPim-1 (Phospho-Tyr309) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2173-pim-1-phospho-tyr309-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPim-1 (Phospho-Tyr309) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-alpha-phospho-thr638-colorimetric-cell-based-elisa-kit-ekc2174-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2174-pkc-alpha-phospho-thr638-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC alpha (Phospho-Thr638) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-delta-phospho-thr505-colorimetric-cell-based-elisa-kit-ekc2175-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC delta (Phospho-Thr505) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2175-pkc-delta-phospho-thr505-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC delta (Phospho-Thr505) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-theta-phospho-thr538-colorimetric-cell-based-elisa-kit-ekc2176-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC theta (Phospho-Thr538) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2176-pkc-theta-phospho-thr538-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC theta (Phospho-Thr538) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/plcbeta3-phospho-ser537-colorimetric-cell-based-elisa-kit-ekc2177-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPLCbeta3 (Phospho-Ser537) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2177-plcbeta3-phospho-ser537-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPLCbeta3 (Phospho-Ser537) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/sgk-phospho-ser78-colorimetric-cell-based-elisa-kit-ekc2178-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSGK (Phospho-Ser78) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2178-sgk-phospho-ser78-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSGK (Phospho-Ser78) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/ship1-phospho-tyr1021-colorimetric-cell-based-elisa-kit-ekc2179-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSHIP1 (Phospho-Tyr1021) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2179-ship1-phospho-tyr1021-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSHIP1 (Phospho-Tyr1021) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with TNF 200NG/ML 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/yap-phospho-ser127-colorimetric-cell-based-elisa-kit-ekc2180-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngYAP (Phospho-Ser127) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2180-yap-phospho-ser127-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgYAP (Phospho-Ser127) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with HU 2nM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/14-3-3-theta-tau-phospho-ser232-colorimetric-cell-based-elisa-kit-ekc2181-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.png14-3-3 theta/tau (Phospho-Ser232) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2181-14-3-3-theta-tau-phospho-ser232-colorimetric-cell-based-elisa-kit-wb-testing-1.jpg14-3-3 theta/tau (Phospho-Ser232) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/14-3-3-zeta-delta-phospho-thr232-colorimetric-cell-based-elisa-kit-ekc2182-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.png14-3-3 zeta/delta (Phospho-Thr232) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2182-14-3-3-zeta-delta-phospho-thr232-colorimetric-cell-based-elisa-kit-wb-testing-1.jpg14-3-3 zeta/delta (Phospho-Thr232) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/53bp1-phospho-ser6-colorimetric-cell-based-elisa-kit-ekc2183-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.png53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2183-53bp1-phospho-ser6-colorimetric-cell-based-elisa-kit-wb-testing-1.jpg53BP1 (Phospho-Ser6) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with insulin 0.01U/ML 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pfkfb2-phospho-ser483-colorimetric-cell-based-elisa-kit-ekc2184-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPFKFB2 (Phospho-Ser483) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2184-pfkfb2-phospho-ser483-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPFKFB2 (Phospho-Ser483) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Heat shock
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https://www.bosterbio.com/products/cell-based-elisa-kits/ahr-phospho-ser36-colorimetric-cell-based-elisa-kit-ekc2186-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAhR (Phospho-Ser36) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2186-ahr-phospho-ser36-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAhR (Phospho-Ser36) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/alox5-phospho-ser271-colorimetric-cell-based-elisa-kit-ekc2187-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2187-alox5-phospho-ser271-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgALOX5 (Phospho-Ser271) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/a-raf-phospho-tyr302-colorimetric-cell-based-elisa-kit-ekc2188-boster.html2026-03-24T05:25:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngA-RAF (Phospho-Tyr302) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2188-a-raf-phospho-tyr302-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgA-RAF (Phospho-Tyr302) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/b-raf-phospho-ser602-colorimetric-cell-based-elisa-kit-ekc2192-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngB-RAF (Phospho-Ser602) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2192-b-raf-phospho-ser602-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgB-RAF (Phospho-Ser602) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/breast-tumor-kinase-phospho-tyr447-colorimetric-cell-based-elisa-kit-ekc2193-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBreast Tumor Kinase (Phospho-Tyr447) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2193-breast-tumor-kinase-phospho-tyr447-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBreast Tumor Kinase (Phospho-Tyr447) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/chk2-phospho-thr383-colorimetric-cell-based-elisa-kit-ekc2194-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk2 (Phospho-Thr383) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2194-chk2-phospho-thr383-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk2 (Phospho-Thr383) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/etk-phospho-tyr566-colorimetric-cell-based-elisa-kit-ekc2195-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngETK (Phospho-Tyr566) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2195-etk-phospho-tyr566-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgETK (Phospho-Tyr566) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/phospho-gab1-tyr659-colorimetric-cell-based-elisa-kit-ekc2196-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPhospho-GAB1 (Tyr659) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2196-phospho-gab1-tyr659-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPhospho-GAB1 (Tyr659) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/hdac1-phospho-ser421-colorimetric-cell-based-elisa-kit-ekc2197-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHDAC1 (Phospho-Ser421) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2197-hdac1-phospho-ser421-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHDAC1 (Phospho-Ser421) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/p38-mapk-phospho-tyr322-colorimetric-cell-based-elisa-kit-ekc2198-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp38 MAPK (Phospho-Tyr322) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2198-p38-mapk-phospho-tyr322-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp38 MAPK (Phospho-Tyr322) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/p38-mapk-phospho-thr179-tyr181-colorimetric-cell-based-elisa-kit-ekc2199-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp38 MAPK (Phospho-Thr179+Tyr181) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-alpha-phospho-tyr657-colorimetric-cell-based-elisa-kit-ekc2200-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC alpha (Phospho-Tyr657) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2200-pkc-alpha-phospho-tyr657-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC alpha (Phospho-Tyr657) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-delta-phospho-tyr52-colorimetric-cell-based-elisa-kit-ekc2201-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC delta (Phospho-Tyr52) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2201-pkc-delta-phospho-tyr52-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC delta (Phospho-Tyr52) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-epsilon-phospho-ser729-colorimetric-cell-based-elisa-kit-ekc2202-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC epsilon (Phospho-Ser729) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2202-pkc-epsilon-phospho-ser729-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC epsilon (Phospho-Ser729) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-theta-phospho-ser695-colorimetric-cell-based-elisa-kit-ekc2203-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC theta (Phospho-Ser695) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2203-pkc-theta-phospho-ser695-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC theta (Phospho-Ser695) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkc-zeta-phospho-thr560-colorimetric-cell-based-elisa-kit-ekc2204-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKC zeta (Phospho-Thr560) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2204-pkc-zeta-phospho-thr560-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKC zeta (Phospho-Thr560) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkd1-pkc-mu-phospho-ser205-colorimetric-cell-based-elisa-kit-ekc2205-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKD1/PKC mu (Phospho-Ser205) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2205-pkd1-pkc-mu-phospho-ser205-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKD1/PKC mu (Phospho-Ser205) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with Anisomycin 25ug/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pkd1-pkc-mu-phospho-tyr463-colorimetric-cell-based-elisa-kit-ekc2206-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKD1/PKC mu (Phospho-Tyr463) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2206-pkd1-pkc-mu-phospho-tyr463-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKD1/PKC mu (Phospho-Tyr463) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pkd2-phospho-ser876-colorimetric-cell-based-elisa-kit-ekc2207-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKD2 (Phospho-Ser876) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2207-pkd2-phospho-ser876-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKD2 (Phospho-Ser876) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with PMA 250ng/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/ptp1b-phospho-ser50-colorimetric-cell-based-elisa-kit-ekc2208-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPTP1B (Phospho-Ser50) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2208-ptp1b-phospho-ser50-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPTP1B (Phospho-Ser50) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/retinoblastoma-phospho-ser811-colorimetric-cell-based-elisa-kit-ekc2209-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRetinoblastoma (Phospho-Ser811) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2209-retinoblastoma-phospho-ser811-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRetinoblastoma (Phospho-Ser811) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tip60-phospho-ser86-colorimetric-cell-based-elisa-kit-ekc2210-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTIP60 (Phospho-Ser86) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2210-tip60-phospho-ser86-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTIP60 (Phospho-Ser86) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tnni3-phospho-ser43-colorimetric-cell-based-elisa-kit-ekc2211-boster.html2026-03-24T05:25:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTNNI3 (Phospho-Ser43) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2211-tnni3-phospho-ser43-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTNNI3 (Phospho-Ser43) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-phospho-tyr326-colorimetric-cell-based-elisa-kit-ekc2212-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 (Phospho-Tyr326) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2212-akt1-phospho-tyr326-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 (Phospho-Tyr326) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse liver
https://www.bosterbio.com/products/cell-based-elisa-kits/apc-phospho-ser2054-colorimetric-cell-based-elisa-kit-ekc2213-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAPC (Phospho-Ser2054) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2213-apc-phospho-ser2054-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAPC (Phospho-Ser2054) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/atf2-phospho-ser480-colorimetric-cell-based-elisa-kit-ekc2214-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATF2 (Phospho-Ser480) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2214-atf2-phospho-ser480-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgATF2 (Phospho-Ser480) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with Anisomycin 25ug/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/bad-phospho-ser134-colorimetric-cell-based-elisa-kit-ekc2216-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBAD (Phospho-Ser134) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2216-bad-phospho-ser134-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBAD (Phospho-Ser134) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse liver
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https://www.bosterbio.com/products/cell-based-elisa-kits/camk4-phospho-thr196-200-colorimetric-cell-based-elisa-kit-ekc2223-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaMK4 (Phospho-Thr196/200) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2223-camk4-phospho-thr196-200-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaMK4 (Phospho-Thr196/200) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with H2O2 100uM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/caspase-9-phospho-tyr153-colorimetric-cell-based-elisa-kit-ekc2224-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaspase 9 (Phospho-Tyr153) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2224-caspase-9-phospho-tyr153-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaspase 9 (Phospho-Tyr153) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COLO205 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/caspase-9-phospho-ser144-colorimetric-cell-based-elisa-kit-ekc2225-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaspase 9 (Phospho-Ser144) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2225-caspase-9-phospho-ser144-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaspase 9 (Phospho-Ser144) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/caspase-9-phospho-ser196-colorimetric-cell-based-elisa-kit-ekc2226-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaspase 9 (Phospho-Ser196) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2226-caspase-9-phospho-ser196-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaspase 9 (Phospho-Ser196) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Calyculin 50nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/caspase-3-phospho-ser150-colorimetric-cell-based-elisa-kit-ekc2227-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCaspase 3 (Phospho-Ser150) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2227-caspase-3-phospho-ser150-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCaspase 3 (Phospho-Ser150) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with Etoposide 25uM 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/catenin-beta-phospho-tyr654-colorimetric-cell-based-elisa-kit-ekc2228-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCatenin-beta (Phospho-Tyr654) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2228-catenin-beta-phospho-tyr654-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCatenin-beta (Phospho-Tyr654) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cd226-phospho-ser329-colorimetric-cell-based-elisa-kit-ekc2229-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD226 (Phospho-Ser329) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2229-cd226-phospho-ser329-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCD226 (Phospho-Ser329) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cd5-phospho-tyr453-colorimetric-cell-based-elisa-kit-ekc2230-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD5 (Phospho-Tyr453) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2230-cd5-phospho-tyr453-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCD5 (Phospho-Tyr453) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/cd71-tfr-phospho-ser24-colorimetric-cell-based-elisa-kit-ekc2231-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCD71/TfR (Phospho-Ser24) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2231-cd71-tfr-phospho-ser24-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCD71/TfR (Phospho-Ser24) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/cdc25a-phospho-ser124-colorimetric-cell-based-elisa-kit-ekc2232-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDC25A (Phospho-Ser124) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2232-cdc25a-phospho-ser124-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDC25A (Phospho-Ser124) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/cdc25b-phospho-ser353-colorimetric-cell-based-elisa-kit-ekc2233-boster.html2026-03-24T05:25:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDC25B (Phospho-Ser353) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2233-cdc25b-phospho-ser353-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDC25B (Phospho-Ser353) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with etoposide 25uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/cdk7-phospho-thr170-colorimetric-cell-based-elisa-kit-ekc2234-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK7 (Phospho-Thr170) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2234-cdk7-phospho-thr170-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDK7 (Phospho-Thr170) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Calyculin A 50nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/chk1-phospho-ser286-colorimetric-cell-based-elisa-kit-ekc2235-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk1 (Phospho-Ser286) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2235-chk1-phospho-ser286-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk1 (Phospho-Ser286) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Na3VO4 0.3uM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/chk1-phospho-ser301-colorimetric-cell-based-elisa-kit-ekc2236-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk1 (Phospho-Ser301) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2236-chk1-phospho-ser301-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk1 (Phospho-Ser301) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ckii-beta-phospho-ser209-colorimetric-cell-based-elisa-kit-ekc2237-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCKII-beta (Phospho-Ser209) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/csfr-phospho-tyr561-colorimetric-cell-based-elisa-kit-ekc2238-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2238-csfr-phospho-tyr561-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/cxcr4-phospho-ser339-colorimetric-cell-based-elisa-kit-ekc2239-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCXCR4 (Phospho-Ser339) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2239-cxcr4-phospho-ser339-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCXCR4 (Phospho-Ser339) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with etoposide 25uM 24H
https://www.bosterbio.com/products/cell-based-elisa-kits/cyclin-h-phospho-thr315-colorimetric-cell-based-elisa-kit-ekc2240-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCyclin H (Phospho-Thr315) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2240-cyclin-h-phospho-thr315-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCyclin H (Phospho-Thr315) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/keratin-18-phospho-ser52-colorimetric-cell-based-elisa-kit-ekc2241-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKeratin 18 (Phospho-Ser52) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2241-keratin-18-phospho-ser52-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgKeratin 18 (Phospho-Ser52) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/keratin-8-phospho-ser432-colorimetric-cell-based-elisa-kit-ekc2242-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngKeratin 8 (Phospho-Ser432) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2242-keratin-8-phospho-ser432-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgKeratin 8 (Phospho-Ser432) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/catenin-delta1-phospho-tyr228-colorimetric-cell-based-elisa-kit-ekc2243-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCatenin-delta1 (Phospho-Tyr228) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2243-catenin-delta1-phospho-tyr228-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCatenin-delta1 (Phospho-Tyr228) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/cryab-phospho-ser19-colorimetric-cell-based-elisa-kit-ekc2245-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCRYAB (Phospho-Ser19) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2245-cryab-phospho-ser19-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCRYAB (Phospho-Ser19) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h
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https://www.bosterbio.com/products/cell-based-elisa-kits/dapk3-phospho-thr265-colorimetric-cell-based-elisa-kit-ekc2247-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDAPK3 (Phospho-Thr265) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2247-dapk3-phospho-thr265-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDAPK3 (Phospho-Thr265) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/dapp1-phospho-tyr139-colorimetric-cell-based-elisa-kit-ekc2248-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2248-dapp1-phospho-tyr139-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Insulin 0.01U/ml 2'
https://www.bosterbio.com/products/cell-based-elisa-kits/dematin-phospho-ser403-colorimetric-cell-based-elisa-kit-ekc2249-boster.html2026-03-24T05:25:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDematin (Phospho-Ser403) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2249-dematin-phospho-ser403-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDematin (Phospho-Ser403) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Insulin 0.01U/ml 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/hrs-phospho-tyr334-colorimetric-cell-based-elisa-kit-ekc2293-boster.html2026-03-24T05:25:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRS (Phospho-Tyr334) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2293-hrs-phospho-tyr334-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHRS (Phospho-Tyr334) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/rad52-phospho-tyr104-colorimetric-cell-based-elisa-kit-ekc2310-boster.html2026-03-24T05:25:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRAD52 (Phospho-Tyr104) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2310-rad52-phospho-tyr104-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRAD52 (Phospho-Tyr104) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with H2O2 100uM 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/cdk5-phospho-tyr15-colorimetric-cell-based-elisa-kit-ekc2315-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDK5 (Phospho-Tyr15) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2315-cdk5-phospho-tyr15-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDK5 (Phospho-Tyr15) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30' and 293 cells treated with H2O2 100u
https://www.bosterbio.com/products/cell-based-elisa-kits/enos-phospho-ser615-colorimetric-cell-based-elisa-kit-ekc2316-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngeNOS (Phospho-Ser615) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2316-enos-phospho-ser615-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgeNOS (Phospho-Ser615) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with EGF 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/foxo4-phospho-thr451-colorimetric-cell-based-elisa-kit-ekc2317-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXO4 (Phospho-Thr451) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2317-foxo4-phospho-thr451-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFOXO4 (Phospho-Thr451) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with EGF 200ng/ml 5'
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https://www.bosterbio.com/products/cell-based-elisa-kits/mtor-phospho-thr2446-colorimetric-cell-based-elisa-kit-ekc2319-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngmTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2319-mtor-phospho-thr2446-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgmTOR (Phospho-Thr2446) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/nek9-phospho-thr210-colorimetric-cell-based-elisa-kit-ekc2320-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNEK9 (Phospho-Thr210) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2320-nek9-phospho-thr210-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNEK9 (Phospho-Thr210) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/p47-phox-phospho-ser304-colorimetric-cell-based-elisa-kit-ekc2321-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp47 phox (Phospho-Ser304) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2321-p47-phox-phospho-ser304-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp47 phox (Phospho-Ser304) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 15' and A549 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/nipa-phospho-ser354-colorimetric-cell-based-elisa-kit-ekc2322-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNIPA (Phospho-Ser354) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2322-nipa-phospho-ser354-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNIPA (Phospho-Ser354) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with HU 2nM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/npm-phospho-thr234-colorimetric-cell-based-elisa-kit-ekc2323-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNPM (Phospho-Thr234) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2323-npm-phospho-thr234-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNPM (Phospho-Thr234) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h
https://www.bosterbio.com/products/cell-based-elisa-kits/p47-phox-phospho-ser359-colorimetric-cell-based-elisa-kit-ekc2324-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp47 phox (Phospho-Ser359) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2324-p47-phox-phospho-ser359-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp47 phox (Phospho-Ser359) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h
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https://www.bosterbio.com/products/cell-based-elisa-kits/pdcd4-phospho-ser457-colorimetric-cell-based-elisa-kit-ekc2326-boster.html2026-03-24T05:25:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPDCD4 (Phospho-Ser457) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2326-pdcd4-phospho-ser457-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPDCD4 (Phospho-Ser457) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with serum 20% 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/ampk1-phospho-ser485-colorimetric-cell-based-elisa-kit-ekc2349-boster.html2026-03-24T05:25:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAMPK1 (Phospho-Ser485) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2349-ampk1-phospho-ser485-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAMPK1 (Phospho-Ser485) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with heat shock
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https://www.bosterbio.com/products/cell-based-elisa-kits/bcr-phospho-tyr177-colorimetric-cell-based-elisa-kit-ekc2365-boster.html2026-03-24T05:25:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBcr (Phospho-Tyr177) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2365-bcr-phospho-tyr177-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBcr (Phospho-Tyr177) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/chk1-phospho-ser280-colorimetric-cell-based-elisa-kit-ekc2371-boster.html2026-03-24T05:25:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk1 (Phospho-Ser280) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2371-chk1-phospho-ser280-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk1 (Phospho-Ser280) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Hu 2nM 24hours
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https://www.bosterbio.com/products/cell-based-elisa-kits/chk2-phospho-ser516-colorimetric-cell-based-elisa-kit-ekc2373-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk2 (Phospho-Ser516) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2373-chk2-phospho-ser516-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk2 (Phospho-Ser516) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with UV
https://www.bosterbio.com/products/cell-based-elisa-kits/chk2-phospho-thr68-colorimetric-cell-based-elisa-kit-ekc2374-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngChk2 (Phospho-Thr68) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2374-chk2-phospho-thr68-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgChk2 (Phospho-Thr68) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV
https://www.bosterbio.com/products/cell-based-elisa-kits/c-jun-phospho-ser63-colorimetric-cell-based-elisa-kit-ekc2375-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngc-Jun (Phospho-Ser63) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2375-c-jun-phospho-ser63-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgc-Jun (Phospho-Ser63) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with UV
https://www.bosterbio.com/products/cell-based-elisa-kits/c-jun-phospho-ser73-colorimetric-cell-based-elisa-kit-ekc2376-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngc-Jun (Phospho-Ser73) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2376-c-jun-phospho-ser73-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgc-Jun (Phospho-Ser73) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 5' and K562 cells treated with TNF 20ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/cofilin-phospho-ser3-colorimetric-cell-based-elisa-kit-ekc2377-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCofilin (Phospho-Ser3) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2377-cofilin-phospho-ser3-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCofilin (Phospho-Ser3) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with paclitaxel 1uM 24hours
https://www.bosterbio.com/products/cell-based-elisa-kits/connexin-43-phospho-ser367-colorimetric-cell-based-elisa-kit-ekc2378-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngConnexin 43 (Phospho-Ser367) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2378-connexin-43-phospho-ser367-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgConnexin 43 (Phospho-Ser367) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with PMA 200ng/ml 10'
https://www.bosterbio.com/products/cell-based-elisa-kits/cortactin-phospho-tyr421-colorimetric-cell-based-elisa-kit-ekc2379-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCortactin (Phospho-Tyr421) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2379-cortactin-phospho-tyr421-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCortactin (Phospho-Tyr421) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with H2O2
https://www.bosterbio.com/products/cell-based-elisa-kits/cortactin-phospho-tyr466-colorimetric-cell-based-elisa-kit-ekc2380-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCortactin (Phospho-Tyr466) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2380-cortactin-phospho-tyr466-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCortactin (Phospho-Tyr466) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/dab1-phospho-tyr232-colorimetric-cell-based-elisa-kit-ekc2383-boster.html2026-03-24T05:25:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDab1 (Phospho-Tyr232) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2383-dab1-phospho-tyr232-colorimetric-cell-based-elisa-kit-ekc2383-wb-testing-1.jpgDab1 (Phospho-Tyr232) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from LOVO cellshttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/e/cell-based-anti-gapdh-control.pngDab1 (Phospho-Tyr232) Colorimetric Cell-Based ELISA Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/a/dab1-phospho-antibody-boster-ekc2383.pngDab1 (Phospho-Tyr232) Colorimetric Cell-Based ELISA Kit
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https://www.bosterbio.com/products/cell-based-elisa-kits/elk1-phospho-ser389-colorimetric-cell-based-elisa-kit-ekc2396-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngElk1 (Phospho-Ser389) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2396-elk1-phospho-ser389-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgElk1 (Phospho-Ser389) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with UV 15' and HeLa cells treated with paclitaxel 1uM 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/estrogen-receptor-alpha-phospho-ser167-colorimetric-cell-based-elisa-kit-ekc2404-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEstrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2404-estrogen-receptor-alpha-phospho-ser167-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEstrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from MCF7 cells treated with EGF
https://www.bosterbio.com/products/cell-based-elisa-kits/ezrin-phospho-tyr353-colorimetric-cell-based-elisa-kit-ekc2405-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEzrin (Phospho-Tyr353) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2405-ezrin-phospho-tyr353-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEzrin (Phospho-Tyr353) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A431 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-tyr397-colorimetric-cell-based-elisa-kit-ekc2406-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2406-fak-phospho-tyr397-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Tyr397) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with Ca2+ 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-tyr861-colorimetric-cell-based-elisa-kit-ekc2407-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Tyr861) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2407-fak-phospho-tyr861-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Tyr861) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-tyr925-colorimetric-cell-based-elisa-kit-ekc2408-boster.html2026-03-24T05:25:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Tyr925) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2408-fak-phospho-tyr925-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Tyr925) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat and HepG2 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/integrin-beta3-phospho-tyr773-colorimetric-cell-based-elisa-kit-ekc2435-boster.html2026-03-24T05:25:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIntegrin beta3 (Phospho-Tyr773) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2435-integrin-beta3-phospho-tyr773-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIntegrin beta3 (Phospho-Tyr773) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HL-60 cells treated with H2O2
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https://www.bosterbio.com/products/cell-based-elisa-kits/relb-phospho-ser573-colorimetric-cell-based-elisa-kit-ekc2505-boster.html2026-03-24T05:25:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRelB (Phospho-Ser573) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2505-relb-phospho-ser573-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRelB (Phospho-Ser573) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from LOVO cells and RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/ret-phospho-tyr1062-colorimetric-cell-based-elisa-kit-ekc2506-boster.html2026-03-24T05:25:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRet (Phospho-Tyr1062) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2506-ret-phospho-tyr1062-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRet (Phospho-Tyr1062) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/smc1-phospho-ser957-colorimetric-cell-based-elisa-kit-ekc2510-boster.html2026-03-24T05:25:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSMC1 (Phospho-Ser957) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2510-smc1-phospho-ser957-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSMC1 (Phospho-Ser957) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with EGF 200ng/ml 5'/HeLa cells treated with EGF 200ng/ml 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/src-phospho-tyr529-colorimetric-cell-based-elisa-kit-ekc2512-boster.html2026-03-24T05:25:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSrc (Phospho-Tyr529) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2512-src-phospho-tyr529-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSrc (Phospho-Tyr529) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with PMA 125ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/stat1-phospho-tyr701-colorimetric-cell-based-elisa-kit-ekc2514-boster.html2026-03-24T05:25:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT1 (Phospho-Tyr701) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2514-stat1-phospho-tyr701-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT1 (Phospho-Tyr701) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stat3-phospho-ser727-colorimetric-cell-based-elisa-kit-ekc2515-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT3 (Phospho-Ser727) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2515-stat3-phospho-ser727-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT3 (Phospho-Ser727) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stat3-phospho-tyr705-colorimetric-cell-based-elisa-kit-ekc2516-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2516-stat3-phospho-tyr705-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stat5a-phospho-ser780-colorimetric-cell-based-elisa-kit-ekc2517-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT5A (Phospho-Ser780) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2517-stat5a-phospho-ser780-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT5A (Phospho-Ser780) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stat5a-b-phospho-tyr694-colorimetric-cell-based-elisa-kit-ekc2518-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT5A/B (Phospho-Tyr694) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2518-stat5a-b-phospho-tyr694-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT5A/B (Phospho-Tyr694) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with EGF
https://www.bosterbio.com/products/cell-based-elisa-kits/stat6-phospho-thr645-colorimetric-cell-based-elisa-kit-ekc2519-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSTAT6 (Phospho-Thr645) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2519-stat6-phospho-thr645-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSTAT6 (Phospho-Thr645) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with IL-4
https://www.bosterbio.com/products/cell-based-elisa-kits/stathmin-1-phospho-ser15-colorimetric-cell-based-elisa-kit-ekc2521-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngStathmin 1 (Phospho-Ser15) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2521-stathmin-1-phospho-ser15-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgStathmin 1 (Phospho-Ser15) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/stathmin-1-phospho-ser24-colorimetric-cell-based-elisa-kit-ekc2522-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngStathmin 1 (Phospho-Ser24) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2522-stathmin-1-phospho-ser24-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgStathmin 1 (Phospho-Ser24) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with PMA 1ng/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/stathmin-1-phospho-ser37-colorimetric-cell-based-elisa-kit-ekc2523-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngStathmin 1 (Phospho-Ser37) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2523-stathmin-1-phospho-ser37-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgStathmin 1 (Phospho-Ser37) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with nocodazole
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https://www.bosterbio.com/products/cell-based-elisa-kits/synuclein-alpha-phospho-tyr133-colorimetric-cell-based-elisa-kit-ekc2525-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSynuclein-alpha (Phospho-Tyr133) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2525-synuclein-alpha-phospho-tyr133-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSynuclein-alpha (Phospho-Tyr133) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with Etoposide 25 muM 60'
https://www.bosterbio.com/products/cell-based-elisa-kits/synuclein-alpha-phospho-tyr136-colorimetric-cell-based-elisa-kit-ekc2526-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSynuclein-alpha (Phospho-Tyr136) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2526-synuclein-alpha-phospho-tyr136-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSynuclein-alpha (Phospho-Tyr136) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser214-colorimetric-cell-based-elisa-kit-ekc2527-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser214) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2527-tau-phospho-ser214-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser214) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser235-colorimetric-cell-based-elisa-kit-ekc2528-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser235) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2528-tau-phospho-ser235-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser235) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser262-colorimetric-cell-based-elisa-kit-ekc2529-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser262) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2529-tau-phospho-ser262-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser262) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser396-colorimetric-cell-based-elisa-kit-ekc2531-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser396) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2531-tau-phospho-ser396-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser396) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser404-colorimetric-cell-based-elisa-kit-ekc2532-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser404) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2532-tau-phospho-ser404-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser404) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with UV
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser422-colorimetric-cell-based-elisa-kit-ekc2533-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser422) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2533-tau-phospho-ser422-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser422) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-thr181-colorimetric-cell-based-elisa-kit-ekc2534-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Thr181) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2534-tau-phospho-thr181-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Thr181) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-thr205-colorimetric-cell-based-elisa-kit-ekc2535-boster.html2026-03-24T05:25:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Thr205) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2535-tau-phospho-thr205-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Thr205) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells and 293 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-thr231-colorimetric-cell-based-elisa-kit-ekc2536-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Thr231) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2536-tau-phospho-thr231-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Thr231) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from MDA-MB-435 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/vasp-phospho-ser238-colorimetric-cell-based-elisa-kit-ekc2537-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVASP (Phospho-Ser238) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2537-vasp-phospho-ser238-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVASP (Phospho-Ser238) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/vegfr2-phospho-tyr1175-colorimetric-cell-based-elisa-kit-ekc2538-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVEGFR2 (Phospho-Tyr1175) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2538-vegfr2-phospho-tyr1175-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVEGFR2 (Phospho-Tyr1175) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells and NIH/3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/vegfr2-phospho-tyr1214-colorimetric-cell-based-elisa-kit-ekc2539-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVEGFR2 (Phospho-Tyr1214) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2539-vegfr2-phospho-tyr1214-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVEGFR2 (Phospho-Tyr1214) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/zap-70-phospho-tyr493-colorimetric-cell-based-elisa-kit-ekc2540-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngZAP-70 (Phospho-Tyr493) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2540-zap-70-phospho-tyr493-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgZAP-70 (Phospho-Tyr493) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells
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https://www.bosterbio.com/products/cell-based-elisa-kits/catenin-beta-phospho-tyr489-colorimetric-cell-based-elisa-kit-ekc2542-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCatenin-beta (Phospho-Tyr489) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2542-catenin-beta-phospho-tyr489-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCatenin-beta (Phospho-Tyr489) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/cdc25a-phospho-ser178-colorimetric-cell-based-elisa-kit-ekc2543-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2543-cdc25a-phospho-ser178-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCDC25A (Phospho-Ser178) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from A549 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/creb-phospho-thr100-colorimetric-cell-based-elisa-kit-ekc2544-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCREB (Phospho-Thr100) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2544-creb-phospho-thr100-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCREB (Phospho-Thr100) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HuvEc cells treated with etoposide 25uM 24H
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-ser695-colorimetric-cell-based-elisa-kit-ekc2545-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2545-egfr-phospho-ser695-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TSA 400nM 24H
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-ser1026-colorimetric-cell-based-elisa-kit-ekc2546-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Ser1026) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2546-egfr-phospho-ser1026-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Ser1026) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with TSA 400nM 24H
https://www.bosterbio.com/products/cell-based-elisa-kits/egfr-phospho-ser1071-colorimetric-cell-based-elisa-kit-ekc2547-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEGFR (Phospho-Ser1071) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2547-egfr-phospho-ser1071-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEGFR (Phospho-Ser1071) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/ezrin-phospho-tyr478-colorimetric-cell-based-elisa-kit-ekc2548-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngEzrin (Phospho-Tyr478) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2548-ezrin-phospho-tyr478-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgEzrin (Phospho-Tyr478) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Na3VO4 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/fak-phospho-ser843-colorimetric-cell-based-elisa-kit-ekc2549-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFAK (Phospho-Ser843) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2549-fak-phospho-ser843-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFAK (Phospho-Ser843) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 20'
https://www.bosterbio.com/products/cell-based-elisa-kits/foxo4-phospho-ser262-colorimetric-cell-based-elisa-kit-ekc2550-boster.html2026-03-24T05:25:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOXO4 (Phospho-Ser262) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2550-foxo4-phospho-ser262-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFOXO4 (Phospho-Ser262) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/her2-phospho-tyr1112-colorimetric-cell-based-elisa-kit-ekc2551-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHER2 (Phospho-Tyr1112) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2551-her2-phospho-tyr1112-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHER2 (Phospho-Tyr1112) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 20'
https://www.bosterbio.com/products/cell-based-elisa-kits/hsf1-phospho-ser121-colorimetric-cell-based-elisa-kit-ekc2552-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHSF1 (Phospho-Ser121) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2552-hsf1-phospho-ser121-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHSF1 (Phospho-Ser121) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with TNF 2500U/ML 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-thr534-217-colorimetric-cell-based-elisa-kit-ekc2553-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Thr534/217) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2553-tau-phospho-thr534-217-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Thr534/217) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from rat brain
https://www.bosterbio.com/products/cell-based-elisa-kits/trk-a-phospho-tyr701-colorimetric-cell-based-elisa-kit-ekc2554-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTrk A (Phospho-Tyr701) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2554-trk-a-phospho-tyr701-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTrk A (Phospho-Tyr701) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
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https://www.bosterbio.com/products/cell-based-elisa-kits/abl1-phospho-thr735-colorimetric-cell-based-elisa-kit-ekc2556-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngABL1 (Phospho-Thr735) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2556-abl1-phospho-thr735-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgABL1 (Phospho-Thr735) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30'
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https://www.bosterbio.com/products/cell-based-elisa-kits/fer-phospho-tyr402-colorimetric-cell-based-elisa-kit-ekc2561-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFER (Phospho-Tyr402) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2561-fer-phospho-tyr402-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFER (Phospho-Tyr402) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with starved 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/flt3-phospho-tyr969-colorimetric-cell-based-elisa-kit-ekc2563-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFLT3 (Phospho-Tyr969) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2563-flt3-phospho-tyr969-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFLT3 (Phospho-Tyr969) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3mM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/ick-phospho-tyr159-colorimetric-cell-based-elisa-kit-ekc2564-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngICK (Phospho-Tyr159) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2564-ick-phospho-tyr159-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgICK (Phospho-Tyr159) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with starved 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/map3k7-phospho-thr187-colorimetric-cell-based-elisa-kit-ekc2566-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP3K7 (Phospho-Thr187) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2566-map3k7-phospho-thr187-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMAP3K7 (Phospho-Thr187) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with heat shock
https://www.bosterbio.com/products/cell-based-elisa-kits/map3k7-phospho-ser439-colorimetric-cell-based-elisa-kit-ekc2567-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP3K7 (Phospho-Ser439) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2567-map3k7-phospho-ser439-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMAP3K7 (Phospho-Ser439) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/mkk3-phospho-thr222-colorimetric-cell-based-elisa-kit-ekc2568-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMKK3 (Phospho-Thr222) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2568-mkk3-phospho-thr222-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMKK3 (Phospho-Thr222) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/map2k7-phospho-ser271-colorimetric-cell-based-elisa-kit-ekc2569-boster.html2026-03-24T05:25:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP2K7 (Phospho-Ser271) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2569-map2k7-phospho-ser271-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMAP2K7 (Phospho-Ser271) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with insulin 0.01U/ml 15' and NIH-3T3 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/map2k7-phospho-thr275-colorimetric-cell-based-elisa-kit-ekc2570-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAP2K7 (Phospho-Thr275) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2570-map2k7-phospho-thr275-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMAP2K7 (Phospho-Thr275) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with calyculinA 50ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/mnk1-phospho-thr255-colorimetric-cell-based-elisa-kit-ekc2571-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMNK1 (Phospho-Thr255) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2571-mnk1-phospho-thr255-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMNK1 (Phospho-Thr255) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Adriamycin 0.5ug/ml 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/msk1-phospho-ser212-colorimetric-cell-based-elisa-kit-ekc2572-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMSK1 (Phospho-Ser212) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2572-msk1-phospho-ser212-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMSK1 (Phospho-Ser212) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/p70-s6-kinase-beta-phospho-ser423-colorimetric-cell-based-elisa-kit-ekc2573-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp70 S6 Kinase beta (Phospho-Ser423) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2573-p70-s6-kinase-beta-phospho-ser423-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp70 S6 Kinase beta (Phospho-Ser423) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with EGF 200ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/pak1-phospho-ser204-colorimetric-cell-based-elisa-kit-ekc2574-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPAK1 (Phospho-Ser204) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2574-pak1-phospho-ser204-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPAK1 (Phospho-Ser204) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/pak2-phospho-ser192-colorimetric-cell-based-elisa-kit-ekc2575-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPAK2 (Phospho-Ser192) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2575-pak2-phospho-ser192-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPAK2 (Phospho-Ser192) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/pak2-phospho-ser141-colorimetric-cell-based-elisa-kit-ekc2576-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPAK2 (Phospho-Ser141) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2576-pak2-phospho-ser141-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPAK2 (Phospho-Ser141) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Adriamycin 0.5uM 24h/Jurkat cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pak3-phospho-ser154-colorimetric-cell-based-elisa-kit-ekc2577-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPAK3 (Phospho-Ser154) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2577-pak3-phospho-ser154-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPAK3 (Phospho-Ser154) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from rat heart
https://www.bosterbio.com/products/cell-based-elisa-kits/pkcb-phospho-ser661-colorimetric-cell-based-elisa-kit-ekc2578-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKCB (Phospho-Ser661) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2578-pkcb-phospho-ser661-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKCB (Phospho-Ser661) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with heat shock
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https://www.bosterbio.com/products/cell-based-elisa-kits/pkr-phospho-thr258-colorimetric-cell-based-elisa-kit-ekc2580-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPKR (Phospho-Thr258) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2580-pkr-phospho-thr258-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPKR (Phospho-Thr258) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with starved 24h
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https://www.bosterbio.com/products/cell-based-elisa-kits/ripk2-phospho-ser176-colorimetric-cell-based-elisa-kit-ekc2582-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2582-ripk2-phospho-ser176-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRIPK2 (Phospho-Ser176) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with UV 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/blnk-phospho-tyr84-colorimetric-cell-based-elisa-kit-ekc2589-boster.html2026-03-24T05:25:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBLNK (Phospho-Tyr84) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2589-blnk-phospho-tyr84-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBLNK (Phospho-Tyr84) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/irs-1-phospho-tyr896-colorimetric-cell-based-elisa-kit-ekc2590-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIRS-1 (Phospho-Tyr896) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2590-irs-1-phospho-tyr896-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIRS-1 (Phospho-Tyr896) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3mM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/top2a-phospho-thr1343-colorimetric-cell-based-elisa-kit-ekc2591-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTOP2A (Phospho-Thr1343) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2591-top2a-phospho-thr1343-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTOP2A (Phospho-Thr1343) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with Ca2+ 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/fos-phospho-thr232-colorimetric-cell-based-elisa-kit-ekc2592-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFOS (Phospho-Thr232) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2592-fos-phospho-thr232-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFOS (Phospho-Thr232) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/dynamin-1-phospho-ser778-colorimetric-cell-based-elisa-kit-ekc2593-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngDynamin-1 (Phospho-Ser778) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2593-dynamin-1-phospho-ser778-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgDynamin-1 (Phospho-Ser778) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/bl-cam-phospho-tyr807-colorimetric-cell-based-elisa-kit-ekc2594-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBL-CAM (Phospho-Tyr807) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2594-bl-cam-phospho-tyr807-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBL-CAM (Phospho-Tyr807) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Na3VO4 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/hp1gamma-phospho-ser93-colorimetric-cell-based-elisa-kit-ekc2595-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHP1gamma (Phospho-Ser93) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2595-hp1gamma-phospho-ser93-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHP1gamma (Phospho-Ser93) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with forskolin 40nM 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/src-phospho-tyr216-colorimetric-cell-based-elisa-kit-ekc2596-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSrc (Phospho-Tyr216) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2596-src-phospho-tyr216-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSrc (Phospho-Tyr216) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/frs2-phospho-tyr436-colorimetric-cell-based-elisa-kit-ekc2597-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFRS2 (Phospho-Tyr436) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2597-frs2-phospho-tyr436-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFRS2 (Phospho-Tyr436) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COS7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/grb2-phospho-ser159-colorimetric-cell-based-elisa-kit-ekc2598-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRB2 (Phospho-Ser159) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2598-grb2-phospho-ser159-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgGRB2 (Phospho-Ser159) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/nmdar2b-phospho-tyr1336-colorimetric-cell-based-elisa-kit-ekc2599-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNMDAR2B (Phospho-Tyr1336) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2599-nmdar2b-phospho-tyr1336-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNMDAR2B (Phospho-Tyr1336) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with TNF 20ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/pyk2-phospho-tyr579-colorimetric-cell-based-elisa-kit-ekc2600-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPYK2 (Phospho-Tyr579) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2600-pyk2-phospho-tyr579-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPYK2 (Phospho-Tyr579) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/wwox-phospho-tyr33-colorimetric-cell-based-elisa-kit-ekc2601-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngWWOX (Phospho-Tyr33) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2601-wwox-phospho-tyr33-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgWWOX (Phospho-Tyr33) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/grk2-phospho-ser685-colorimetric-cell-based-elisa-kit-ekc2602-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGRK2 (Phospho-Ser685) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2602-grk2-phospho-ser685-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgGRK2 (Phospho-Ser685) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with insulin 0.01U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/aire-phospho-ser156-colorimetric-cell-based-elisa-kit-ekc2603-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAIRE (Phospho-Ser156) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2603-aire-phospho-ser156-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAIRE (Phospho-Ser156) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with Hu 2nM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/aml1-phospho-ser435-colorimetric-cell-based-elisa-kit-ekc2604-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAML1 (Phospho-Ser435) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2604-aml1-phospho-ser435-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAML1 (Phospho-Ser435) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/atrip-phospho-ser224-colorimetric-cell-based-elisa-kit-ekc2605-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngATRIP (Phospho-Ser224) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2605-atrip-phospho-ser224-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgATRIP (Phospho-Ser224) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/b-raf-phospho-thr753-colorimetric-cell-based-elisa-kit-ekc2606-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngB-Raf (Phospho-Thr753) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2606-b-raf-phospho-thr753-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgB-Raf (Phospho-Thr753) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/brca1-phospho-ser1457-colorimetric-cell-based-elisa-kit-ekc2607-boster.html2026-03-24T05:25:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2607-brca1-phospho-ser1457-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgBRCA1 (Phospho-Ser1457) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from 293 cells treated with epo 20U/ml 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/claudin-3-phospho-tyr219-colorimetric-cell-based-elisa-kit-ekc2608-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngClaudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2608-claudin-3-phospho-tyr219-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgClaudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COLO205 cells treated with EGF 200ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/claudin-6-phospho-tyr219-colorimetric-cell-based-elisa-kit-ekc2609-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngClaudin 6 (Phospho-Tyr219) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2609-claudin-6-phospho-tyr219-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgClaudin 6 (Phospho-Tyr219) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells treated with UV 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/claudin-7-phospho-tyr210-colorimetric-cell-based-elisa-kit-ekc2610-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngClaudin 7 (Phospho-Tyr210) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2610-claudin-7-phospho-tyr210-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgClaudin 7 (Phospho-Tyr210) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from rat liver
https://www.bosterbio.com/products/cell-based-elisa-kits/crmp2-phospho-thr509-colorimetric-cell-based-elisa-kit-ekc2611-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCRMP2 (Phospho-Thr509) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2611-crmp2-phospho-thr509-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCRMP2 (Phospho-Thr509) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with heat shock
https://www.bosterbio.com/products/cell-based-elisa-kits/ctbp1-phospho-ser422-colorimetric-cell-based-elisa-kit-ekc2612-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngCtBP1 (Phospho-Ser422) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2612-ctbp1-phospho-ser422-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgCtBP1 (Phospho-Ser422) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with TNF 20ng/ml 30' and Jurkat cells treated with UV 15'
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https://www.bosterbio.com/products/cell-based-elisa-kits/mapkapk5-phospho-thr182-colorimetric-cell-based-elisa-kit-ekc2617-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMAPKAPK5 (Phospho-Thr182) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2617-mapkapk5-phospho-thr182-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMAPKAPK5 (Phospho-Thr182) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with Na3VO4 0.3nM 40'
https://www.bosterbio.com/products/cell-based-elisa-kits/mef2c-phospho-ser396-colorimetric-cell-based-elisa-kit-ekc2618-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMEF2C (Phospho-Ser396) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2618-mef2c-phospho-ser396-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMEF2C (Phospho-Ser396) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/mkp-1-2-phospho-ser296-318-colorimetric-cell-based-elisa-kit-ekc2619-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMKP-1/2 (Phospho-Ser296/318) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/mnk1-phospho-thr385-colorimetric-cell-based-elisa-kit-ekc2620-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMNK1 (Phospho-Thr385) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2620-mnk1-phospho-thr385-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgMNK1 (Phospho-Thr385) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from COLO205 cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/nudc-phospho-ser326-colorimetric-cell-based-elisa-kit-ekc2621-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNudC (Phospho-Ser326) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2621-nudc-phospho-ser326-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNudC (Phospho-Ser326) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from rat brain
https://www.bosterbio.com/products/cell-based-elisa-kits/psen1-phospho-ser357-colorimetric-cell-based-elisa-kit-ekc2622-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngPSEN1 (Phospho-Ser357) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2622-psen1-phospho-ser357-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgPSEN1 (Phospho-Ser357) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells treated with UV 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/rfa2-phospho-ser33-colorimetric-cell-based-elisa-kit-ekc2623-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRFA2 (Phospho-Ser33) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2623-rfa2-phospho-ser33-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRFA2 (Phospho-Ser33) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with Adriamycin 0.5ug/ml 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/rfwd2-phospho-ser387-colorimetric-cell-based-elisa-kit-ekc2624-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRFWD2 (Phospho-Ser387) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2624-rfwd2-phospho-ser387-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgRFWD2 (Phospho-Ser387) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/s6-ribosomal-protein-phospho-ser235-ser236-colorimetric-cell-based-elisa-kit-ekc2625-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngS6 Ribosomal Protein (Phospho-Ser235+Ser236) Colorimetric Cell-Based ELISA KitBoster Kit Box
https://www.bosterbio.com/products/cell-based-elisa-kits/srebp-1-phospho-ser439-colorimetric-cell-based-elisa-kit-ekc2626-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSREBP-1 (Phospho-Ser439) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2626-srebp-1-phospho-ser439-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgSREBP-1 (Phospho-Ser439) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with TNF 20ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/tif-ia-phospho-ser649-colorimetric-cell-based-elisa-kit-ekc2627-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTIF-IA (Phospho-Ser649) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2627-tif-ia-phospho-ser649-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTIF-IA (Phospho-Ser649) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/tob1-phospho-ser164-colorimetric-cell-based-elisa-kit-ekc2628-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTOB1 (Phospho-Ser164) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2628-tob1-phospho-ser164-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTOB1 (Phospho-Ser164) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HT29 cells treated with serum 20% 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/top2a-phospho-ser1525-colorimetric-cell-based-elisa-kit-ekc2629-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTOP2A (Phospho-Ser1525) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2629-top2a-phospho-ser1525-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTOP2A (Phospho-Ser1525) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from RAW264.7 cells treated with TNF 20ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/vcp-phospho-ser352-colorimetric-cell-based-elisa-kit-ekc2630-boster.html2026-03-24T05:25:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngVCP (Phospho-Ser352) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2630-vcp-phospho-ser352-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgVCP (Phospho-Ser352) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from NIH-3T3 cells treated with starved 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/her3-phospho-tyr1197-colorimetric-cell-based-elisa-kit-ekc2631-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHER3 (Phospho-Tyr1197) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2631-her3-phospho-tyr1197-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHER3 (Phospho-Tyr1197) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HepG2 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/lamin-a-c-phospho-ser22-colorimetric-cell-based-elisa-kit-ekc2633-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngLamin A/C (Phospho-Ser22) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2633-lamin-a-c-phospho-ser22-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgLamin A/C (Phospho-Ser22) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with paclitaxel 1uM 24h
https://www.bosterbio.com/products/cell-based-elisa-kits/p63-phospho-ser395-colorimetric-cell-based-elisa-kit-ekc2634-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngp63 (Phospho-Ser395) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2634-p63-phospho-ser395-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgp63 (Phospho-Ser395) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from LOVO cells treated with nocodazole 1ug/ml 18h
https://www.bosterbio.com/products/cell-based-elisa-kits/akt1-phospho-thr308-colorimetric-cell-based-elisa-kit-ekc2635-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAkt1 (Phospho-Thr308) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2635-akt1-phospho-thr308-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgAkt1 (Phospho-Thr308) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 5'
https://www.bosterbio.com/products/cell-based-elisa-kits/fadd-phospho-ser191-colorimetric-cell-based-elisa-kit-ekc2636-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngFADD (Phospho-Ser191) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2636-fadd-phospho-ser191-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgFADD (Phospho-Ser191) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with PMA 125ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/nmdar2b-phospho-ser1303-colorimetric-cell-based-elisa-kit-ekc2637-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngNMDAR2B (Phospho-Ser1303) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2637-nmdar2b-phospho-ser1303-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgNMDAR2B (Phospho-Ser1303) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from Jurkat cells treated with TNF 20ng/ml 30'
https://www.bosterbio.com/products/cell-based-elisa-kits/hdac7a-phospho-ser155-colorimetric-cell-based-elisa-kit-ekc2638-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHDAC7A (Phospho-Ser155) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2638-hdac7a-phospho-ser155-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgHDAC7A (Phospho-Ser155) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells
https://www.bosterbio.com/products/cell-based-elisa-kits/integrin-beta1-phospho-tyr795-colorimetric-cell-based-elisa-kit-ekc2639-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngIntegrin beta1 (Phospho-Tyr795) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2639-integrin-beta1-phospho-tyr795-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgIntegrin beta1 (Phospho-Tyr795) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from HeLa cells treated with UV 15'
https://www.bosterbio.com/products/cell-based-elisa-kits/tau-phospho-ser516-199-colorimetric-cell-based-elisa-kit-ekc2640-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngTau (Phospho-Ser516/199) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2640-tau-phospho-ser516-199-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgTau (Phospho-Ser516/199) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from mouse brain
https://www.bosterbio.com/products/cell-based-elisa-kits/c-raf-phospho-ser642-colorimetric-cell-based-elisa-kit-ekc2641-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngC-RAF (Phospho-Ser642) Colorimetric Cell-Based ELISA KitBoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2641-c-raf-phospho-ser642-colorimetric-cell-based-elisa-kit-wb-testing-1.jpgC-RAF (Phospho-Ser642) Colorimetric Cell-Based ELISA KitWestern blot analysis of extracts from K562 and HuvEc cells treated with PMA
https://www.bosterbio.com/products/abl-phospho-tyr393-412-colorimetric-cell-based-elisa-ekc2642-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAbl (Phospho-Tyr393/412) Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2642-abl-phospho-tyr393-412-colorimetric-cell-based-elisa-wb-testing-1.jpgAbl (Phospho-Tyr393/412) Colorimetric Cell-Based ELISAWestern blot analysis of lysates from RAW264.7 cells
https://www.bosterbio.com/products/cell-based-elisa-kits/abl-phospho-tyr412-colorimetric-cell-based-elisa-kit-ekc2643-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAbl (Phospho-Tyr412) Colorimetric Cell-Based ELISABoster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ekc2643-abl-phospho-tyr412-colorimetric-cell-based-elisa-wb-testing-1.jpgAbl (Phospho-Tyr412) Colorimetric Cell-Based ELISAWestern blot analysis of lysates from COS7 cells treated with Adriamycin 0.5ug/ml 24h
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-brg1-picoband-trade-antibody-monoclonal-m00223-1-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-wb-testing-1.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) Western blot analysis of BRG1 using anti-BRG1 antibody (M00223-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Raji whole cell lysates;<br>
Lane 2: human K562 whole cell lysates;<br>
Lane 3: human HL-60 whole cell lysates;<br>
Lane 4: human Caco-2 whole cell lysates;<br>
Lane 5: human Hela whole cell lysates;<br>
Lane 6: human HepG2 whole cell lysates;<br>
Lane 7: rat brain tissue lysates;<br>
Lane 8: mouse brain tissue lysates;<br>
Lane 9: mouse lung tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BRG1 antigen affinity purified monoclonal antibody (Catalog # M00223-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BRG1 at approximately 181KD. The expected band size for BRG1 is at 181KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-ihc-testing-2.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) IHC analysis of BRG1 using anti-BRG1 antibody (M00223-1). <br>
BRG1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BRG1 Antibody (M00223-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-ihc-testing-3.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) IHC analysis of BRG1 using anti-BRG1 antibody (M00223-1). <br>
BRG1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BRG1 Antibody (M00223-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-ihc-testing-4.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) IHC analysis of BRG1 using anti-BRG1 antibody (M00223-1). <br>
BRG1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BRG1 Antibody (M00223-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-ihc-testing-5.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) IHC analysis of BRG1 using anti-BRG1 antibody (M00223-1). <br>
BRG1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BRG1 Antibody (M00223-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-1-brg1-primary-antibodies-fcm-testing-6.jpgAnti-BRG1 SMARCA4 Antibody Picoband® (monoclonal, 3F4) Flow Cytometry analysis of U20S cells using anti- BRG1 antibody (M00223-1). <br>Overlay histogram showing U20S cells stained with M00223-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- BRG1 Antibody (M00223-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adk-picoband-trade-antibody-monoclonal-m02193-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-adk-primary-antibodies-wb-testing-1.jpgAnti-ADK Antibody Picoband® (monoclonal, 7F4) Western blot analysis of ADK using anti-ADK antibody (M02193). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human SW620 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADK antigen affinity purified monoclonal antibody (Catalog # M02193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ADK at approximately 45KD. The expected band size for ADK is at 45KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-adk-primary-antibodies-ihc-testing-2.jpgAnti-ADK Antibody Picoband® (monoclonal, 7F4) IHC analysis of ADK using anti-ADK antibody (M02193). <br>
ADK was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADK Antibody (M02193) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-adk-primary-antibodies-ihc-testing-3.jpgAnti-ADK Antibody Picoband® (monoclonal, 7F4) IHC analysis of ADK using anti-ADK antibody (M02193). <br>
ADK was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADK Antibody (M02193) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-adk-primary-antibodies-ihc-testing-4.jpgAnti-ADK Antibody Picoband® (monoclonal, 7F4) IHC analysis of ADK using anti-ADK antibody (M02193). <br>
ADK was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ADK Antibody (M02193) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-adk-primary-antibodies-fcm-testing-5.pngAnti-ADK Antibody Picoband® (monoclonal, 7F4) Flow Cytometry analysis of PC-3 cells using anti- ADK antibody (M02193). <br>Overlay histogram showing PC-3 cells stained with M02193 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ADK Antibody (M02193, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cox-iv-picoband-trade-antibody-monoclonal-m05442-1-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-cox_iv-primary-antibodies-wb-testing-1.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11) Western blot analysis of COX IV using anti-COX IV antibody (M05442-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEPG2 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human T47D whole cell lysates, <br>
Lane 5: human CACO-2 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates, <br>
Lane 8: rat brain tissue lysates, <br>
Lane 9: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-COX IV antigen affinity purified monoclonal antibody (Catalog # M05442-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-fsn3-13-e70973-g006.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11)The results of western blot showed that protein expression levels of Cox4I1, Uqcr11, Atp5g1, and Atp5d were significantly lower in the Ctrl group than in the HFHS–POF group. (* p < 0.05 vs. Ctrl group; ** p < 0.01 vs. Ctrl group; t ‐test, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12445113/'>40979580</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-cox_iv-primary-antibodies-ihc-testing-2.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11) IHC analysis of COX IV using anti-COX IV antibody (M05442-1). <br>
COX IV was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COX IV Antibody (M05442-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-cox_iv-primary-antibodies-ihc-testing-3.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11) IHC analysis of COX IV using anti-COX IV antibody (M05442-1). <br>
COX IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COX IV Antibody (M05442-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-cox_iv-primary-antibodies-ihc-testing-4.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11) IHC analysis of COX IV using anti-COX IV antibody (M05442-1). <br>
COX IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COX IV Antibody (M05442-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-1-cox_iv-primary-antibodies-fcm-testing-5_1.jpgAnti-COX IV COX4I1 Antibody Picoband® (monoclonal, 4G11) Flow Cytometry analysis of U937 cells using anti- COX IV antibody (M05442-1). <br>
Overlay histogram showing U937 cells stained with M05442-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COX IV Antibody (M05442-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-his-tag-picoband-trade-antibody-monoclonal-m30975-1-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30975-1-his-tag-primary-antibodies-wb-testing-1.jpgAnti-His Tag Antibody Picoband® (monoclonal, 19b10) Western blot analysis of His Tag using anti-His Tag antibody (M30975-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br>
Lane 1: recombinant mouse His Tag protein 10ng, <br>
Lane 1: recombinant mouse His Tag protein 5ng, <br>
Lane 1: recombinant mouse His Tag protein 2.5ng, <br>
Lane 1: recombinant mouse His Tag protein 1.25ng. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # M30975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30975-1-fmicb-14-1335658-g001.jpgAnti-His Tag Antibody Picoband® (monoclonal, 19b10)Purification and identification of rLP78. (A) SDS-PAGE analysis of the purification of rLP78 by Ni + column. Lane M, protein molecular weight marker; Lane 1, the supernatant of the whole bacterial lysate; Lane 2, elution with 50 mM of imidazole; Lane 3, elution with 500 mM of imidazole. (B) Western blotting analysis of the purified rLP78 using anti-His-tag monoclonal antibody. (C) Western blotting analysis of the purified rLP78 using mouse anti- M. synoviae polyclonal antibodies.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10803467/&orig_host=www.ncbi.nlm.nih.gov'>38264482</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30975-1-fmicb-14-1335658-g006.jpgAnti-His Tag Antibody Picoband® (monoclonal, 19b10)Activity of rLP78 to adhere to membrane proteins of DF-1 cells. Microtiter ELISA plates were coated with the extracted membrane protein, cytosolic protein or BSA. (A) Different concentrations of rLP78 were added to individual wells. Bound proteins were detected using a mouse anti-His-tag monoclonal antibody. (B) Adherence of rLP78 (100 μg/mL) to membrane proteins of DF-1 cells was inhibited by mouse anti-LP78 serum and further detected using goat anti-mouse IgG. Bars represent the mean ± standard deviation of the OD 450nm values of samples in triplicate. The differences were compared between each group. The same letter indicates no obvious difference ( p > 0.05); different letters indicate a significant difference ( p < 0.05).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10803467/&orig_host=www.ncbi.nlm.nih.gov'>38264482</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30975-1-fmicb-14-1335658-g008.jpgAnti-His Tag Antibody Picoband® (monoclonal, 19b10)Binding ability of rLP78 to human fibronectin (hFn) and human plasminogen (hPlg). The binding ability of rLP78 to hFn (A) or hPlg (B) was identified by ELISA. The plates were coated with 5 μg/mL of hFn or hPlg. Different concentrations of rLP78 or BSA were added to individual wells. Bound proteins were detected using a mouse anti-His-tag monoclonal antibody. Bars represent the mean ± standard deviation of the OD values of samples in triplicate. The differences were compared between each group. The same letter indicates no obvious difference ( p > 0.05); different letters indicate a significant difference ( p < 0.01). Binding ability of rLP78 to hFn (C) or hPlg (D) was confirmed by western blotting. Bound hFn was determined by rabbit anti-hFn antibody or rabbit anti-plasminogen antibody. BSA was chosen as negative control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10803467/&orig_host=www.ncbi.nlm.nih.gov'>38264482</a></b>
https://www.bosterbio.com/picokine-elisa-kits/rat-serpin-a12-picokine-elisa-kit-ek1741-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1741.pngRat Serpin A12 ELISA Kit PicoKine®Rat Serpin A12 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-pam-picokine-elisa-kit-ek1765-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1765.pngHuman PAM ELISA Kit PicoKine®Human PAM PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-tinagl1-picokine-elisa-kit-ek1766-boster.html2026-03-24T05:25:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1766.pngHuman TINAGL1 ELISA Kit PicoKine®Human TINAGL1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl17-picokine-elisa-kit-ek1768-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1768.pngMouse CXCL17 ELISA Kit PicoKine®Mouse CXCL17 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-il-1racp-il-1r3-il1rap-picokine-elisa-kit-ek1769-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1769.jpgHuman IL-1RAcP/IL-1R3/IL1RAP ELISA Kit PicoKine®Human IL-1RAcP/IL-1R3/IL1RAP PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-contactin-1-picokine-elisa-kit-ek1773-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1773.pngRat Contactin-1 ELISA Kit PicoKine®Rat Contactin-1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-contactin-2-picokine-elisa-kit-ek1774-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1774.jpgHuman Contactin-2 ELISA Kit PicoKine®Human Contactin-2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-contactin-4-picokine-elisa-kit-ek1780-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1780.jpgHuman Contactin-4 ELISA Kit PicoKine®Human Contactin-4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-contactin-4-picokine-elisa-kit-ek1781-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1781.jpgMouse Contactin-4 ELISA Kit PicoKine®Mouse Contactin-4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-contactin-5-picokine-elisa-kit-ek1782-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1782.jpgHuman Contactin-5 ELISA Kit PicoKine®Human Contactin-5 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-contactin-6-picokine-elisa-kit-ek1783-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1783.pngMouse Contactin-6 ELISA Kit PicoKine®Mouse Contactin-6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-epha4-picokine-elisa-kit-ek1799-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1799.pngMouse EphA4 ELISA Kit PicoKine®Mouse EphA4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-epha4-picokine-elisa-kit-ek1800-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1800.pngRat EphA4 ELISA Kit PicoKine®Rat EphA4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd155-pvr-picokine-elisa-kit-ek1831-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1831.pngHuman CD155/PVR/Poliovirus receptor ELISA Kit PicoKine®Human CD155/PVR PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-sez6l-picokine-elisa-kit-ek1832-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1832.pngRat SEZ6L ELISA Kit PicoKine®Rat SEZ6L PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-ca12-picokine-elisa-kit-ek1833-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1833_1.pngMouse CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®Mouse CA12 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-spock1-testican-1-picokine-elisa-kit-ek1848-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1848.pngHuman SPOCK1/Testican-1 ELISA Kit PicoKine®Human SPOCK1/Testican-1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-omentin-intelectin-1-picokine-elisa-kit-ek1849-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1849.pngHuman Omentin/intelectin-1 ELISA Kit PicoKine®Human Omentin/intelectin-1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd200r1-picokine-elisa-kit-ek1850-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1850.pngHuman CD200R1 ELISA Kit PicoKine®Human CD200R1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-edar-picokine-elisa-kit-ek1851-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1851.pngHuman EDAR ELISA Kit PicoKine®Human EDAR PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-brorin-picokine-elisa-kit-ek1852-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1852.pngHuman Brorin ELISA Kit PicoKine®Human Brorin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-sectm1a-picokine-elisa-kit-ek1853-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1853.pngMouse SECTM1A ELISA Kit PicoKine®Mouse SECTM1A PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-pdgf-cc-picokine-elisa-kit-ek1854-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1854.pngMouse PDGF-CC ELISA Kit PicoKine®Mouse PDGF-CC PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-pdgf-cc-picokine-elisa-kit-ek1855-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1855.pngRat PDGF-CC ELISA Kit PicoKine®Rat PDGF-CC PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-eda-picokine-trade-elisa-kit-ek1856-boster.html2026-03-24T05:25:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1856.jpgHuman EDA ELISA Kit PicoKine®Human EDA PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-slit2-picokine-elisa-kit-ek1860-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1860.pngMouse SLIT2 ELISA Kit PicoKine®Mouse SLIT2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-sez6l-picokine-elisa-kit-ek1863-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1863.jpgMouse SEZ6L ELISA Kit PicoKine®Mouse SEZ6L PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd300b-picokine-elisa-kit-ek1865-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1865.pngMouse CD300b ELISA Kit PicoKine®Mouse CD300b PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-slitrk1-picokine-elisa-kit-ek1866-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1866.pngHuman SLITRK1 ELISA Kit PicoKine®Human SLITRK1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-slitrk6-picokine-elisa-kit-ek1868-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1868.pngHuman SLITRK6 ELISA Kit PicoKine®Human SLITRK6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-akr1c3-picokine-elisa-kit-ek1869-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1869.pngHuman AKR1C3 ELISA Kit PicoKine®Human AKR1C3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-akr1c4-picokine-elisa-kit-ek1870-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1870.pngHuman AKR1C4 ELISA Kit PicoKine®Human AKR1C4 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-podocalyxin-picokine-elisa-kit-ek1899-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1899.pngMouse Podocalyxin ELISA Kit PicoKine®Mouse Podocalyxin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-creg1-picokine-elisa-kit-ek1950-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1950.pngMouse CREG1 ELISA Kit PicoKine®Mouse CREG1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-renalase-picokine-elisa-kit-ek1951-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1951.pngHuman Renalase ELISA Kit PicoKine®Human Renalase PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-gif-picokine-elisa-kit-ek1952-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1952.pngHuman GIF ELISA Kit PicoKine®Human GIF PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-chordin-picokine-elisa-kit-ek2086-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2086.pngRat Chordin ELISA Kit PicoKine®Rat Chordin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-pth1r-picokine-elisa-kit-ek2087-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2087.pngHuman PTH1R ELISA Kit PicoKine®Human PTH1R PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-crtac1-picokine-elisa-kit-ek2088-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2088.jpgHuman CRTAC1 ELISA Kit PicoKine®Human CRTAC1 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd99-picokine-elisa-kit-ek2089-boster.html2026-03-24T05:25:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2089.jpgMouse CD99 ELISA Kit PicoKine®Mouse CD99 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cd99l2-picokine-elisa-kit-ek2090-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2090.jpgHuman CD99L2 ELISA Kit PicoKine®Human CD99L2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd99l2-picokine-elisa-kit-ek2091-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2091.jpgMouse CD99L2 ELISA Kit PicoKine®Mouse CD99L2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-il4r-picokine-elisa-kit-ek2092-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2092.jpgHuman IL4R ELISA Kit PicoKine®Human IL4R PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-il4r-picokine-elisa-kit-ek2093-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2093.pngMouse IL4R ELISA Kit PicoKine®Mouse IL4R PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cadherin-4-r-cadherin-picokine-elisa-kit-ek2094-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2094.pngMouse R-Cadherin-4 Cdh4 ELISA Kit PicoKine®Mouse Cadherin-4/R-Cadherin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-cadherin-4-r-cadherin-picokine-elisa-kit-ek2095-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2095.pngRat R-Cadherin-4 Cdh4 ELISA Kit PicoKine®Rat Cadherin-4/R-Cadherin PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cadherin-6-picokine-elisa-kit-ek2096-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2096.pngHuman Cadherin-6/CDH6 ELISA Kit PicoKine®Human Cadherin-6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/mouse-cadherin-6-picokine-elisa-kit-ek2097-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2097.pngMouse Cadherin-6/CDH6 ELISA Kit PicoKine®Mouse Cadherin-6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-cadherin-6-picokine-elisa-kit-ek2098-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2098.pngRat Cadherin-6/CDH6 ELISA Kit PicoKine®Rat Cadherin-6 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-cadherin-15-picokine-elisa-kit-ek2099-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2099.pngHuman M-Cadherin-15 CDH15 ELISA Kit PicoKine®Human Cadherin-15 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-legumain-picokine-elisa-kit-ek2100-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2100.pngRat Legumain ELISA Kit PicoKine®Rat Legumain PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-epo-picokine-elisa-kit-ek0332-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0332.jpgHuman Erythropoietin/EPO ELISA Kit PicoKine®Human EPO PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-flt3-flk2-picokine-elisa-kit-ek0352-boster.html2026-04-01T05:01:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0352.pngHuman FLT3/FLK2 ELISA Kit PicoKine®Human FLT3/FLK2 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/rat-flt-3-ligand-picokine-elisa-kit-ek2101-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2101.pngRat Flt-3 ligand ELISA Kit PicoKine®Rat Flt-3 ligand PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/picokine-elisa-kits/human-flt4-vegfr3-picokine-elisa-kit-ek0545-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0545.pngHuman FLT4/VEGFR3 ELISA Kit PicoKine®Human FLT4/VEGFR3 PicoKine ELISA Kit Standard Curve
https://www.bosterbio.com/products/primary-antibodies/anti-areb6-zeb1-picoband-trade-antibody-a00548-2-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00548-2-zeb1-primary-antibodies-wb-testing-1.jpgAnti-AREB6/ZEB1 Antibody Picoband® Western blot analysis of ZEB1 using anti-ZEB1 antibody (A00548-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human HeLa whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZEB1 antigen affinity purified polyclonal antibody (Catalog # A00548-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZEB1 at approximately 200 kDa. The expected band size for ZEB1 is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00548-2-ZEB1-primary-antibodies-IHC-testing-2.jpgAnti-AREB6/ZEB1 Antibody Picoband® IHC analysis of ZEB1 using anti-ZEB1 antibody (A00548-2).
<br>
ZEB1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZEB1 Antibody (A00548-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00548-2-zeb1-primary-antibodies-ihc-testing-3.jpgAnti-AREB6/ZEB1 Antibody Picoband® IHC analysis of ZEB1 using anti-ZEB1 antibody (A00548-2).
<br>
ZEB1 was detected in paraffin-embedded section of human melanoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZEB1 Antibody (A00548-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00548-2-ZEB1-primary-antibodies-IF-testing-4.jpgAnti-AREB6/ZEB1 Antibody Picoband® IF analysis of ZEB1 using anti-ZEB1 antibody (A00548-2) <br>
ZEB1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-ZEB1 Antibody (A00548-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00548-2-zeb1-primary-antibodies-fc-testing-5.jpgAnti-AREB6/ZEB1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-ZEB1 antibody (A00548-2).
<br>
Overlay histogram showing K562 cells stained with A00548-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB1 Antibody (A00548-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/acetylcholinesterase-activity-assay-kit-100-assays-ar4001-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar4001-acetylcholinesterase.jpgAcetylcholinesterase Activity Assay Kit - 100 Assays
https://www.bosterbio.com/catalog/product/view/id/551552026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar4002-alamarblue-cell-viability-assay-reagent.jpgAlamarBlue Cell Viability Assay Reagent
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-1-activity-assay-kit-ar4003-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_4.pngCaspase-1 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-2-activity-assay-kit-ar4004-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_4_1.pngCaspase-2 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-3-7-activity-assay-kit-ar4005-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_5.pngCaspase-3, 7 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-4-activity-assay-kit-ar4006-boster.html2026-03-24T05:25:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_1_1.pngCaspase-4 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-8-activity-assay-kit-ar4007-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_2_1.pngCaspase-8 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-9-activity-assay-kit-ar4008-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_3_1.pngCaspase-9 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-6-activity-assay-kit-ar4009-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_4_2.pngCaspase-6 Activity Assay Kit
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/caspase-10-activity-assay-kit-ar4010-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_5_1.pngCaspase-10 Activity Assay Kit
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cofilin-2-cfl2-picoband-trade-antibody-monoclonal-m04773-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-WB-testing-1.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) Western blot analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates<br>
Lane 2: human U2OS whole cell lysates<br>
Lane 3: human HepG2 whole cell lysates<br>
Lane 4: human T-47D whole cell lysates<br>
Lane 5: human Raji whole cell lysates<br>
Lane 6: human placenta tissue lysates<br>
Lane 7: human A549 whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cofilin-2 antigen affinity purified monoclonal antibody (Catalog # M04773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cofilin-2 at approximately 19KD. The expected band size for Cofilin-2 is at 19KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-WB-testing-2.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) Western blot analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: rat heart tissue lysates<br>
Lane 2: rat liver tissue lysates<br>
Lane 3: rat kidney tissue lysates<br>
Lane 4: rat brain tissue lysates<br>
Lane 5: mouse heart tissue lysates<br>
Lane 6: mouse liver tissue lysates<br>
Lane 7: mouse kidney tissue lysates<br>
Lane 8: mouse brain tissue lysates<br>
Lane 9: mouse NIH/3T3 whole cell lysates
<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cofilin-2 antigen affinity purified monoclonal antibody (Catalog # M04773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cofilin-2 at approximately 19KD. The expected band size for Cofilin-2 is at 19KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-IHC-testing-3.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) IHC analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).
<br>
Cofilin-2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cofilin-2 Antibody (M04773) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-IHC-testing-4.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) IHC analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).
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Cofilin-2 was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cofilin-2 Antibody (M04773) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-IHC-testing-5.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) IHC analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).
<br>
Cofilin-2 was detected in paraffin-embedded section of mouse skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cofilin-2 Antibody (M04773) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-IHC-testing-6.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) IHC analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773).
<br>
Cofilin-2 was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cofilin-2 Antibody (M04773) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-IF-testing-7.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) IF analysis of Cofilin-2 using anti-Cofilin-2 antibody (M04773). <br> Cofilin-2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Cofilin-2 Antibody (M04773) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-FC-testing-8.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) Flow Cytometry analysis of A549 cells using anti-Cofilin-2 antibody (M04773).<br>Overlay histogram showing A549 cells stained with M04773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cofilin-2 Antibody (M04773,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04773-Cofilin-2-primary-antibodies-FC-testing-9.jpgAnti-Cofilin 2/CFL2 Antibody Picoband® (monoclonal, 8C13) Flow Cytometry analysis of SiHa cells using anti-Cofilin-2 antibody (M04773).<br>Overlay histogram showing SiHa cells stained with M04773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cofilin-2 Antibody (M04773,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk1-picoband-trade-antibody-monoclonal-m00209-6-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-WB-testing-1.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) Western blot analysis of CDK1 using anti-CDK1 antibody (M00209-6).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human HEK293 whole cell lysates<br>
Lane 2: human A549 whole cell lysates<br>
Lane 3: human HepG2 whole cell lysates<br>
Lane 4: human THP-1 whole cell lysates<br>
Lane 5: human PANC-1 whole cell lysates<br>
Lane 6: human SW620 whole cell lysates<br>
Lane 7: rat RH35 whole cell lysates<br>
Lane 8: mouse NIH/3T3 whole cell lysates
<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK1 antigen affinity purified monoclonal antibody (Catalog # M00209-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34KD. The expected band size for CDK1 is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-IHC-testing-2.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) IHC analysis of CDK1 using anti-CDK1 antibody (M00209-6).
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CDK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CDK1 Antibody (M00209-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-IHC-testing-3.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) IHC analysis of CDK1 using anti-CDK1 antibody (M00209-6).
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CDK1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CDK1 Antibody (M00209-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-IHC-testing-4.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) IHC analysis of CDK1 using anti-CDK1 antibody (M00209-6).
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CDK1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CDK1 Antibody (M00209-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-IHC-testing-5.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) IHC analysis of CDK1 using anti-CDK1 antibody (M00209-6).
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CDK1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CDK1 Antibody (M00209-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-IHC-testing-6.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) IHC analysis of CDK1 using anti-CDK1 antibody (M00209-6).
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CDK1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CDK1 Antibody (M00209-6) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-FC-testing-7.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) Flow Cytometry analysis of PC-3 cells using anti-CDK1 antibody (M00209-6).<br>Overlay histogram showing PC-3 cells stained with M00209-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CDK1 Antibody (M00209-6,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00209-6-CDK1-primary-antibodies-FC-testing-8.jpgAnti-CDK1 Antibody Picoband® (monoclonal, 2G11) Flow Cytometry analysis of U20S cells using anti-CDK1 antibody (M00209-6).<br>Overlay histogram showing U20S cells stained with M00209-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CDK1 Antibody (M00209-6,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bak-bak1-picoband-trade-antibody-monoclonal-m01163-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01163-1-bak1-primary-antibodies-wb-testing-1.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) Western blot analysis of BAK/BAK1 using anti-BAK/BAK1 antibody (M01163-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human A431 whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human MCF-7 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAK/BAK1 antigen affinity purified monoclonal antibody (Catalog # M01163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BAK/BAK1 at approximately 25 kDa. The expected band size for BAK/BAK1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-2.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
<br>
BAK1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-3.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
<br>
BAK1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-4.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
<br>
BAK1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-5.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
<br>
BAK1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-6.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
<br>
BAK1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IHC-testing-7.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IHC analysis of BAK1 using anti-BAK1 antibody (M01163-1).
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BAK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-IF-testing-8.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) IF analysis of BAK1 using anti-BAK1 antibody (M01163-1). <br> BAK1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-BAK1 Antibody (M01163-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01163-1-BAK1-primary-antibodies-FC-testing-9.jpgAnti-BAK/BAK1 Antibody Picoband® (monoclonal, 4C2) Flow Cytometry analysis of PC-3 cells using anti-BAK1 antibody (M01163-1).<br>Overlay histogram showing PC-3 cells stained with M01163-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAK1 Antibody (M01163-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp27-hspb1-picoband-trade-antibody-monoclonal-m00676-5-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00676-5-hsp27-primary-antibodies-wb-testing-1.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) Western blot analysis of HSP27 using anti-HSP27 antibody (M00676-5). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates， <br>
Lane 2: human CACO-2 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP27 antigen affinity purified monoclonal antibody (Catalog # M00676-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP27 at approximately 27 kDa. The expected band size for HSP27 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00676-5-HSP27-primary-antibodies-IHC-testing-2.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) IHC analysis of HSP27 using anti-HSP27 antibody (M00676-5).
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HSP27 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP27 Antibody (M00676-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00676-5-HSP27-primary-antibodies-IHC-testing-3.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) IHC analysis of HSP27 using anti-HSP27 antibody (M00676-5).
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HSP27 was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP27 Antibody (M00676-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00676-5-HSP27-primary-antibodies-IHC-testing-4.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) IHC analysis of HSP27 using anti-HSP27 antibody (M00676-5).
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HSP27 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP27 Antibody (M00676-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00676-5-hsp27-primary-antibodies-if-testing-5.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) IF analysis of HSP27 using anti- HSP27 antibody (M00676-5). <br>
HSP27 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- HSP27 Antibody (M00676-5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00676-5-hsp27-primary-antibodies-fc-testing-6.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) Flow Cytometry analysis of A549 cells using anti-HSP27 antibody (M00676-5).<br>Overlay histogram showing A549 cells stained with M00676-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSP27 Antibody (M00676-5,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00676-5-hsp27-primary-antibodies-fc-testing-7.jpgAnti-Hsp27/HSPB1 Antibody Picoband® (monoclonal, 3H3) Flow Cytometry analysis of U20S cells using anti-HSP27 antibody (M00676-5).<br>Overlay histogram showing U20S cells stained with M00676-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSP27 Antibody (M00676-5,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sae2-uba2-picoband-trade-antibody-monoclonal-m03816-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03816-1-UBA2-primary-antibodies-WB-testing-1.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) Western blot analysis of UBA2 using anti-UBA2 antibody (M03816-1).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human K562 whole cell lysates<br>
Lane 2: human Raji whole cell lysates<br>
Lane 3: human THP-1 whole cell lysates<br>
Lane 4: human SW579 whole cell lysates<br>
Lane 5: human HepG2 whole cell lysates<br>
Lane 6: human CCRF-CEM whole cell lysates<br>
Lane 7: rat PC-12 whole cell lysates<br>
Lane 8: mouse RAW246.7 whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-UBA2 antigen affinity purified monoclonal antibody (Catalog # M03816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for UBA2 at approximately 90KD. The expected band size for UBA2 is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M03816-1-UBA2-primary-antibodies-FC-testing-2.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5H11) Flow Cytometry analysis of A431 cells using anti-UBA2 antibody (M03816-1).<br>Overlay histogram showing A431 cells stained with M03816-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-UBA2 Antibody (M03816-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppcs-picoband-trade-antibody-monoclonal-m05567-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-WB-testing-1.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) Western blot analysis of PPCS using anti-PPCS antibody (M05567).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human A375 whole cell lysates<br>
Lane 2: human COLO-320 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PPCS antigen affinity purified monoclonal antibody (Catalog # M05567) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PPCS at approximately 34KD. The expected band size for PPCS is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-IHC-testing-2.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) IHC analysis of PPCS using anti-PPCS antibody (M05567).
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PPCS was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPCS Antibody (M05567) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-IHC-testing-3.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) IHC analysis of PPCS using anti-PPCS antibody (M05567).
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PPCS was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPCS Antibody (M05567) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-IHC-testing-4.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) IHC analysis of PPCS using anti-PPCS antibody (M05567).
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PPCS was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPCS Antibody (M05567) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-IHC-testing-5.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) IHC analysis of PPCS using anti-PPCS antibody (M05567).
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PPCS was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PPCS Antibody (M05567) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-FC-testing-6.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) Flow Cytometry analysis of PC-3 cells using anti-PPCS antibody (M05567).<br>Overlay histogram showing PC-3 cells stained with M05567 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPCS Antibody (M05567,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M05567-PPCS-primary-antibodies-FC-testing-7.jpgAnti-PPCS Antibody Picoband® (monoclonal, 7G13) Flow Cytometry analysis of SiHa cells using anti-PPCS antibody (M05567).<br>Overlay histogram showing SiHa cells stained with M05567 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PPCS Antibody (M05567,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irs1-picoband-trade-antibody-monoclonal-m00268-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-WB-testing-1.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) Western blot analysis of IRS1 using anti-IRS1 antibody (M00268-1).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human A549 whole cell lysates<br>
Lane 2: human T-47D whole cell lysates<br>
Lane 3: human Caco-2 whole cell lysates<br>
Lane 4: human SW620 whole cell lysates<br>
Lane 5: human Hela whole cell lysates<br>
Lane 6: human Raji whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IRS1 antigen affinity purified monoclonal antibody (Catalog # M00268-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IRS1 at approximately 160-180KD. The expected band size for IRS1 is at 130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-IHC-testing-2.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) IHC analysis of IRS1 using anti-IRS1 antibody (M00268-1).
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IRS1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-IRS1 Antibody (M00268-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-IHC-testing-3.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) IHC analysis of IRS1 using anti-IRS1 antibody (M00268-1).
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IRS1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-IRS1 Antibody (M00268-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-IHC-testing-4.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) IHC analysis of IRS1 using anti-IRS1 antibody (M00268-1).
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IRS1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-IRS1 Antibody (M00268-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-FC-testing-5.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) Flow Cytometry analysis of PC-3 cells using anti-IRS1 antibody (M00268-1).<br>Overlay histogram showing PC-3 cells stained with M00268-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRS1 Antibody (M00268-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00268-1-IRS1-primary-antibodies-FC-testing-6.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) Flow Cytometry analysis of U20S cells using anti-IRS1 antibody (M00268-1).<br>Overlay histogram showing U20S cells stained with M00268-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRS1 Antibody (M00268-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00268-1-irs1-primary-antibodies-if-testing-7.jpgAnti-IRS1 Antibody Picoband® (monoclonal, 10I3) IF analysis of IRS1 using anti-IRS1 antibody (M00268-1). <br>
IRS1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-IRS1 Antibody (M00268-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-hsp60-hspd1-picoband-trade-antibody-monoclonal-m01280-3-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-WB-testing-1.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Caco-2 whole cell lysates<br>
Lane 2: human A549 whole cell lysates<br>
Lane 3: human THP-1 whole cell lysates<br>
Lane 4: human SW620 whole cell lysates<br>
Lane 5: human U-937 whole cell lysates<br>
Lane 6: human HepG2 whole cell lysates<br>
Lane 7: rat RH35 whole cell lysates<br>
Lane 8: mouse RAW246.7 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPD1 antigen affinity purified monoclonal antibody (Catalog # M01280-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPD1 at approximately 60KD. The expected band size for HSPD1 is at 60KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-3-12953_2023_213_fig2_html.pngAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2)The differential abundance of HSP90AB1, HSPD1 and HSPA13 in villi tissues was verified by Western blot. A The representative of Western blot analysis to verify selected differentially expressed proteins HSP90AB1, HSPD1 and HSPA13 in the villi tissue of embryo from EMA ( n = 6) and control( n = 6). B The scatter plot of HSP90AB1 abundance in the villi tissue of embryo from EMA ( n = 22) vs control ( n = 22) ( P < 0.01). C The scatter plot of HSPD1 abundance in the villi tissue of embryo from EMA ( n = 22) vs control ( n = 22) ( P < 0.01). D The scatter plot of HSPA13 abundance in the villi tissue of embryo from EMA ( n = 22) vs control ( n = 22) ( P < 0.01) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12953-023-00213-w'>37587463</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-3-12953_2023_213_fig3_html.pngAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2)A , B , C Immunohistochemical staining for HSP90AB1, HSPD1, and HSPA13 observed in the cytoplasm of syncytiotrophoblast and cytotrophoblast cells (× 200, × 400). Villus from 22 patients with EMA showed lower; D - F Quantitative scoring results of IHC analyses w shown as box plots <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12953-023-00213-w'>37587463</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-IHC-testing-2.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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HSPD1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-IHC-testing-3.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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HSPD1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-IHC-testing-4.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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HSPD1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-IHC-testing-5.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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HSPD1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-IHC-testing-6.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-3).
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HSPD1 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPD1 Antibody (M01280-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-FC-testing-7.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) Flow Cytometry analysis of A431 cells using anti-HSPD1 antibody (M01280-3).<br>Overlay histogram showing A431 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPD1 Antibody (M01280-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01280-3-HSPD1-primary-antibodies-FC-testing-8.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) Flow Cytometry analysis of HepG2 cells using anti-HSPD1 antibody (M01280-3).<br>Overlay histogram showing HepG2 cells stained with M01280-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPD1 Antibody (M01280-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-3-hspd1-primary-antibody-if-testing-9.jpgAnti-Hsp60/HSPD1 Antibody Picoband® (monoclonal, 6G2) IF analysis of Hsp60/HSPD1 using anti-Hsp60/HSPD1 antibody (M01280-3). <br>
Hsp60/HSPD1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-Hsp60/HSPD1 Antibody (M01280-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdhb-picoband-trade-antibody-monoclonal-m01090-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-wb-testing-1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) Western blot analysis of SDHB using anti-SDHB antibody (M01090-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SDHB antigen affinity purified monoclonal antibody (Catalog # M01090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29KD. The expected band size for SDHB is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-ihc-testing-2_1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-1).
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SDHB was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-ihc-testing-3_1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-1).
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SDHB was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-ihc-testing-4_1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-1).
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SDHB was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-ihc-testing-5_1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-1).
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SDHB was detected in section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SDHB Antibody (M01090-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-ihc-testing-6_1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-1).
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SDHB was detected in section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SDHB Antibody (M01090-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-1-sdhb-primary-antibodies-fc-testing-7.jpgAnti-SDHB Antibody Picoband® (monoclonal, 2I3) Flow Cytometry analysis of A549 cells using anti-SDHB antibody (M01090-1).<br>Overlay histogram showing A549 cells stained with M01090-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SDHB Antibody (M01090-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plk1-picoband-trade-antibody-monoclonal-m00182-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00182-PLK1-primary-antibodies-WB-testing-1.jpgAnti-PLK1 Antibody Picoband® (monoclonal, 6D11) Western blot analysis of PLK1 using anti-PLK1 antibody (M00182).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates<br>
Lane 2: monkey COS-7 whole cell lysates<br>
Lane 3: human Raji whole cell lysates<br>
Lane 4: human U2OS whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PLK1 antigen affinity purified monoclonal antibody (Catalog # M00182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68KD. The expected band size for PLK1 is at 68KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmgb1-picoband-trade-antibody-monoclonal-m00066-2-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-WB-testing-1.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) Western blot analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human HepG2 whole cell lysates<br>
Lane 2: human CCRF-CEM whole cell lysates<br>
Lane 3: monkey COS-7 whole cell lysates<br>
Lane 4: human SW620 whole cell lysates<br>
Lane 5: human THP-1 whole cell lysates<br>
Lane 6: rat PC-12 whole cell lysates<br>
Lane 7: rat RH35 whole cell lysates<br>
Lane 8: mouse NIH/3T3 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HMGB1 antigen affinity purified monoclonal antibody (Catalog # M00066-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HMGB1 at approximately 25KD. The expected band size for HMGB1 is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-ihc-testing-10.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2). <br> HMGB1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-wb-testing-2.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) Western blot analysis of HMGB1 using anti-HMGB1 antibody (M00066-2). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse RAW264.7- WT whole cell lysates,<br>
Lane 2: mouse RAW264.7-Hmgb1 KO whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with mouse anti-HMGB1 antigen affinity purified monoclonal antibody (M00066-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1051) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMGB1 at approximately 25 kDa. The expected band size for HMGB1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-3.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-4.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-5.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-6.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-7.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-8.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00066-2-HMGB1-primary-antibodies-IHC-testing-9.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-ihc-testing-11.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2). <br> HMGB1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-ihc-testing-12.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2). <br> HMGB1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-ihc-testing-13.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2). <br> HMGB1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-ihc-testing-14.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) IHC analysis of HMGB1 using anti-HMGB1 antibody (M00066-2).
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HMGB1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB1 Antibody (M00066-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-2-hmgb1-primary-antibodies-fc-testing-15.jpgAnti-HMGB1 Antibody Picoband® (monoclonal, 5H3) Flow Cytometry analysis of SiHa cells using anti-HMGB1 antibody (M00066-2). <br> Overlay histogram showing SiHa cells stained with M00066-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HMGB1 Antibody (M00066-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sqstm1-p62-picoband-trade-antibody-monoclonal-m00300-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-1-sqstm1-primary-antibodies-wb-testing-1.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: human SiHa whole cell lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: rat RH35 whole cell lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates,<br>
Lane 9: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SQSTM1 antigen affinity purified monoclonal antibody (Catalog # M00300-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62 kDa. The expected band size for SQSTM1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-1-ijbsv18p1878g006.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11)MiR-197-3p downregulating HSPA5 modulates autophagy to control X-ray sensitivity. (A) The expression of autophagy related proteins in transfected cells was detected by Western Blot. (B, C) The changes of autophagosomes after transfection with GFP-LC3 were detected. (D) Western Blot experiments verified that miR-197-3p regulated autophagy by targeting HSPA5. (E) The expression of HSPA5 and autophagy related protein LC3B in transfected cells was detected by cell immunofluorescence. (F) Immunofluorescence showed that tumors infected with LV-miR-197-3p expressed HSPA5 and LC3B in animal tissue. (G) Western Blot experiment showed the expression of LC3BI/LC3BII and HSPA5 in animal tumor protein. (H) Immunohistochemical results showed the expression of HSPA5, Ki-67, BCL2, LC3B and P62.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8935226/&orig_host=www.ncbi.nlm.nih.gov'>35342334</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-1-sqstm1-primary-antibodies-ihc-testing-2.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IHC analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1).<br>
SQSTM1 was detected in section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00300-1-SQSTM1-primary-antibodies-IHC-testing-3.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IHC analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1).
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SQSTM1 was detected in section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00300-1-SQSTM1-primary-antibodies-IHC-testing-4.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IHC analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1).
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SQSTM1 was detected in section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00300-1-SQSTM1-primary-antibodies-IHC-testing-5.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IHC analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1).
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SQSTM1 was detected in section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00300-1-SQSTM1-primary-antibodies-IHC-testing-6.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IHC analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1).
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SQSTM1 was detected in section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-1-sqstm1-primary-antibodies-if-testing-7.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IF analysis of SQSTM1 using anti- SQSTM1 antibody (M00300-1). <br>
SQSTM1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- SQSTM1 Antibody (M00300-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00300-1-SQSTM1-primary-antibodies-IF-testing-8.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) IF analysis of SQSTM1 using anti-SQSTM1 antibody (M00300-1). <br> SQSTM1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-SQSTM1 Antibody (M00300-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-1-sqstm1-primary-antibodies-fc-testing-9.jpgAnti-SQSTM1/p62 Antibody Picoband® (monoclonal, 3H11) Flow Cytometry analysis of PC-3 cells using anti-SQSTM1 antibody (M00300-1). <br> Overlay histogram showing PC-3 cells stained with M00300-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SQSTM1 Antibody (M00300-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/protein-and-enzyme-activity-assays/protease-activity-assay-kit-ar4011-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_6.pngProtease Activity Assay Kit
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab27a-picoband-trade-antibody-monoclonal-m01608-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-WB-testing-1.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) Western blot analysis of RAB27A using anti-RAB27A antibody (M01608). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human K562 whole cell lysates<br>
Lane 2: human HepG2 whole cell lysates<br>
Lane 3: human THP-1 whole cell lysates<br>
Lane 4: human HT1080 whole cell lysates<br>
Lane 5: human SW620 whole cell lysates<br>
Lane 6: human PANC-1 whole cell lysates<br>
Lane 7: rat RH35 whole cell lysates<br>
Lane 8: mouse NIH/3T3 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB27A antigen affinity purified monoclonal antibody (Catalog # M01608) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB27A at approximately 27KD. The expected band size for RAB27A is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-IHC-testing-2.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
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RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-IHC-testing-3.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
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RAB27A was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-IHC-testing-4.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
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RAB27A was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-IHC-testing-5.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
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RAB27A was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-IHC-testing-6.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IHC analysis of RAB27A using anti-RAB27A antibody (M01608).
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RAB27A was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RAB27A Antibody (M01608) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-FC-testing-7.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) Flow Cytometry analysis of K562 cells using anti-RAB27A antibody (M01608).
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Overlay histogram showing K562 cells stained with M01608 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB27A Antibody (M01608,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01608-RAB27A-primary-antibodies-FC-testing-8.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) Flow Cytometry analysis of U87 cells using anti-RAB27A antibody (M01608).
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Overlay histogram showing U87 cells stained with M01608 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB27A Antibody (M01608,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01608-rab27a-primary-antibodies-if-testing-9.jpgAnti-RAB27A Antibody Picoband® (monoclonal, 2F5) IF analysis of RAB27A using anti-RAB27A antibody (M01608). <br>
RAB27A was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RAB27A Antibody (M01608) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp105-hsph1-picoband-trade-antibody-monoclonal-m04168-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-WB-testing-1.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) Western blot analysis of HSPH1 using anti-HSPH1 antibody (M04168-1).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Caco-2 whole cell lysates<br>
Lane 2: human K562 whole cell lysates<br>
Lane 3: human A549 whole cell lysates<br>
Lane 4: human HepG2 whole cell lysates<br>
Lane 5: human PANC-1 whole cell lysates<br>
Lane 6: human SGC-7901 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPH1 antigen affinity purified monoclonal antibody (Catalog # M04168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPH1 at approximately 105KD. The expected band size for HSPH1 is at 105KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-IHC-testing-2.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) IHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1).
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HSPH1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPH1 Antibody (M04168-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-IHC-testing-3.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) IHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1).
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HSPH1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPH1 Antibody (M04168-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-IHC-testing-4.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) IHC analysis of HSPH1 using anti-HSPH1 antibody (M04168-1).
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HSPH1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSPH1 Antibody (M04168-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-IF-testing-5.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) IF analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). <br> HSPH1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-HSPH1 Antibody (M04168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-FC-testing-6.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) Flow Cytometry analysis of A431 cells using anti-HSPH1 antibody (M04168-1).<br>Overlay histogram showing A431 cells stained with M04168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPH1 Antibody (M04168-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04168-1-HSPH1-primary-antibodies-FC-testing-7.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) Flow Cytometry analysis of HepG2 cells using anti-HSPH1 antibody (M04168-1).<br>Overlay histogram showing HepG2 cells stained with M04168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HSPH1 Antibody (M04168-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04168-1-hsph1-primary-antibodies-if-testing-8.jpgAnti-Hsp105/HSPH1 Antibody Picoband® (monoclonal, 3D10) IF analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). <br>
HSPH1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-HSPH1 Antibody (M04168-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ran-picoband-trade-antibody-monoclonal-m00204-1-boster.html2026-03-24T05:25:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-1-ran-primary-antibodies-wb-testing-1_1.jpgAnti-Ran Antibody Picoband® (monoclonal, 5D5)Western blot analysis of Ran using anti-Ran antibody (M00204-1).
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Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human A375 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>
Lane 4: human Jurkat whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse testis tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ran antigen affinity purified monoclonal antibody (Catalog # M00204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Ran at approximately 24 kDa. The expected band size for Ran is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-1-ran-primary-antibodies-fc-testing-3.pngAnti-Ran Antibody Picoband® (monoclonal, 5D5) Flow Cytometry analysis of PC-3 cells using anti-Ran antibody (M00204-1). <br> Overlay histogram showing PC-3 cells stained with M00204-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ran Antibody (M00204-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00204-1-ran-primary-antibodies-if-testing-4.jpgAnti-Ran Antibody Picoband® (monoclonal, 5D5) IF analysis of Ran using anti-Ran antibody (M00204-1). <br> Ran was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Ran Antibody (M00204-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pax5-picoband-trade-antibody-monoclonal-m00669-2-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-WB-testing-1.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) Western blot analysis of PAX5 using anti-PAX5 antibody (M00669-2).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Raji whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PAX5 antigen affinity purified monoclonal antibody (Catalog # M00669-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45KD. The expected band size for PAX5 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-IHC-testing-2.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) IHC analysis of PAX5 using anti-PAX5 antibody (M00669-2).
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PAX5 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PAX5 Antibody (M00669-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-IHC-testing-3.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) IHC analysis of PAX5 using anti-PAX5 antibody (M00669-2).
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PAX5 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PAX5 Antibody (M00669-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-IHC-testing-4.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) IHC analysis of PAX5 using anti-PAX5 antibody (M00669-2).
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PAX5 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PAX5 Antibody (M00669-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-IHC-testing-5.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) IHC analysis of PAX5 using anti-PAX5 antibody (M00669-2).
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PAX5 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PAX5 Antibody (M00669-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00669-2-PAX5-primary-antibodies-IF-testing-6.jpgAnti-PAX5 Antibody Picoband® (monoclonal, 6I4) IF analysis of PAX5 using anti-PAX5 antibody (M00669-2). <br> PAX5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PAX5 Antibody (M00669-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cope-picoband-trade-antibody-monoclonal-m04544-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-WB-testing-1.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) Western blot analysis of COPE using anti-COPE antibody (M04544).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human HEK293 whole cell lysates<br>
Lane 2: human HepG2 whole cell lysates<br>
Lane 3: human placenta tissue lysates<br>
Lane 4: human Caco-2 whole cell lysates<br>
Lane 5: human A549 whole cell lysates<br>
Lane 6: human PANC-1 whole cell lysates<br>
Lane 7: human SW579 whole cell lysates <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-COPE antigen affinity purified monoclonal antibody (Catalog # M04544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04544-cope-primary-antibodies-if-testing-10.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IF analysis of COPE using anti-COPE antibody (M04544). <br>
COPE was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-COPE Antibody (M04544) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-2.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-3.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-4.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-5.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-6.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-IHC-testing-7.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) IHC analysis of COPE using anti-COPE antibody (M04544).
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COPE was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COPE Antibody (M04544) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-FC-testing-8.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) Flow Cytometry analysis of A431 cells using anti-COPE antibody (M04544).<br>Overlay histogram showing A431 cells stained with M04544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COPE Antibody (M04544,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M04544-COPE-primary-antibodies-FC-testing-9.jpgAnti-COPE Antibody Picoband® (monoclonal, 9B6) Flow Cytometry analysis of HepG2 cells using anti-COPE antibody (M04544).<br>Overlay histogram showing HepG2 cells stained with M04544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COPE Antibody (M04544,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdhb-picoband-trade-antibody-monoclonal-m01090-2-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-2-sdhb-primary-antibodies-wb-testing-1.jpgAnti-SDHB Antibody Picoband® (monoclonal, 11I3) Western blot analysis of SDHB using anti-SDHB antibody (M01090-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SDHB antigen affinity purified monoclonal antibody (Catalog # M01090-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29KD. The expected band size for SDHB is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-2-sdhb-primary-antibodies-ihc-testing-2.jpgAnti-SDHB Antibody Picoband® (monoclonal, 11I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-2). <br> SDHB was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-2-sdhb-primary-antibodies-ihc-testing-3.jpgAnti-SDHB Antibody Picoband® (monoclonal, 11I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-2). <br> SDHB was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-2-sdhb-primary-antibodies-ihc-testing-4.jpgAnti-SDHB Antibody Picoband® (monoclonal, 11I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-2). <br> SDHB was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-2-sdhb-primary-antibodies-ihc-testing-5.jpgAnti-SDHB Antibody Picoband® (monoclonal, 11I3) IHC analysis of SDHB using anti-SDHB antibody (M01090-2). <br> SDHB was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SDHB Antibody (M01090-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrf1-picoband-trade-antibody-monoclonal-m01129-1-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-wb-testing-1.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) Western blot analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: human SH-SY5Y whole cell lysates, <br>
Lane 6: human THP-1 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NRF1 antigen affinity purified monoclonal antibody (Catalog # M01129-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NRF1 at approximately 54-70 kDa. The expected band size for NRF1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-IHC-testing-2.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1).
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NRF1 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-IHC-testing-3.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1).
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NRF1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-IHC-testing-4.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1).
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NRF1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-IHC-testing-5.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1).
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NRF1 was detected in paraffin-embedded section of human testis cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-IHC-testing-6.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1).
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NRF1 was detected in paraffin-embedded section of human testis cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-7.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti- NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-8.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti- NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01129-1-NRF1-primary-antibodies-FC-testing-9.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) Flow Cytometry analysis of PC-3 cells using anti-NRF1 antibody (M01129-1).<br>Overlay histogram showing PC-3 cells stained with M01129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NRF1 Antibody (M01129-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-if-testing-10.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IF analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-11.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-12.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-13.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-14.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-15.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-16.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-17.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-18.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-19.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-20.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01129-1-nrf1-primary-antibodies-ihc-testing-21.jpgAnti-NRF1 Antibody Picoband® (monoclonal, 2G4) IHC analysis of NRF1 using anti-NRF1 antibody (M01129-1). <br>
NRF1 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NRF1 Antibody (M01129-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmin-picoband-trade-antibody-monoclonal-m01948-3-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-WB-testing-1.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) Western blot analysis of Desmin using anti-Desmin antibody (M01948-3).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: rat heart tissue lysates<br>
Lane 2: rat skeletal muscle tissue lysates<br>
Lane 3: mouse heart tissue lysates<br>
Lane 4: mouse skeletal muscle tissue lysates<br>
Lane 5: human K562 whole cell lysates<br>
Lane 6: rat liver tissue lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54KD. The expected band size for Desmin is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-IHC-testing-2.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) IHC analysis of Desmin using anti-Desmin antibody (M01948-3).<br>
Desmin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Desmin Antibody (M01948-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-IHC-testing-3.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) IHC analysis of Desmin using anti-Desmin antibody (M01948-3).
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Desmin was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Desmin Antibody (M01948-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-IHC-testing-4.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) IHC analysis of Desmin using anti-Desmin antibody (M01948-3).
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Desmin was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Desmin Antibody (M01948-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-IHC-testing-5.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) IHC analysis of Desmin using anti-Desmin antibody (M01948-3).
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Desmin was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Desmin Antibody (M01948-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-IHC-testing-6.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) IHC analysis of Desmin using anti-Desmin antibody (M01948-3).
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Desmin was detected in paraffin-embedded section of mouse skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Desmin Antibody (M01948-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01948-3-Desmin-primary-antibodies-FC-testing-7.jpgAnti-Desmin Antibody Picoband® (monoclonal, 2B5) Flow Cytometry analysis of THP-1 cells using anti-Desmin antibody (M01948-3).<br>Overlay histogram showing THP-1 cells stained with M01948-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Desmin Antibody (M01948-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd43-spn-picoband-trade-antibody-monoclonal-m01296-1-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-WB-testing-1.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) Western blot analysis of CD43 using anti-CD43 antibody (M01296-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human K562 whole cell lysates<br>
Lane 2: human HL-60 whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD43 antigen affinity purified monoclonal antibody (Catalog # M01296-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD43 at approximately 115KD. The expected band size for CD43 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-IHC-testing-2.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) IHC analysis of CD43 using anti-CD43 antibody (M01296-1).
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CD43 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD43 Antibody (M01296-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-IHC-testing-3.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) IHC analysis of CD43 using anti-CD43 antibody (M01296-1).
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CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD43 Antibody (M01296-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-IHC-testing-4.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) IHC analysis of CD43 using anti-CD43 antibody (M01296-1).
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CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD43 Antibody (M01296-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-IHC-testing-5.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) IHC analysis of CD43 using anti-CD43 antibody (M01296-1).
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CD43 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD43 Antibody (M01296-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-IHC-testing-6.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) IHC analysis of CD43 using anti-CD43 antibody (M01296-1).
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CD43 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD43 Antibody (M01296-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M01296-1-CD43-primary-antibodies-FC-testing-7.jpgAnti-CD43/SPN Antibody Picoband® (monoclonal, 4I3)7. Flow Cytometry analysis of human PBMC cells using anti-CD43 antibody (M01296-1).<br>Overlay histogram showing human PBMC cells stained with M01296-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD43 Antibody (M01296-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-comt-picoband-trade-antibody-monoclonal-m00464-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-WB-testing-1.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) Western blot analysis of COMT using anti-COMT antibody (M00464).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human HepG2 whole cell lysates<br>
Lane 2: human K562 whole cell lysates<br>
Lane 3: human THP-1 whole cell lysates<br>
Lane 4: human HEK293 whole cell lysates<br>
Lane 5: human A549 whole cell lysates<br>
Lane 6: human Caco-2 whole cell lysates<br>
Lane 7: rat RH35 whole cell lysates<br>
Lane 8: mouse Neuro-2a whole cell lysates
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-COMT antigen affinity purified monoclonal antibody (Catalog # M00464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for COMT at approximately 29KD. The expected band size for COMT is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-2.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-3.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-4.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-5.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-6.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-7.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IHC-testing-8.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IHC analysis of COMT using anti-COMT antibody (M00464).
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COMT was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-COMT Antibody (M00464) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M00464-COMT-primary-antibodies-IF-testing-9.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IF analysis of COMT using anti-COMT antibody (M00464). <br> COMT was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-COMT Antibody (M00464) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00464-comt-primary-antibodies-fc-testing-10.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) Flow Cytometry analysis of K562 cells using anti-COMT antibody (M00464). <br> Overlay histogram showing K562 cells stained with M00464 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COMT Antibody (M00464,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00464-comt-primary-antibodies-fc-testing-11.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) Flow Cytometry analysis of U87 cells using anti-COMT antibody (M00464). <br> Overlay histogram showing U87 cells stained with M00464 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-COMT Antibody (M00464,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00464-comt-primary-antibodies-if-testing-12.jpgAnti-COMT Antibody Picoband® (monoclonal, 15C10) IF analysis of COMT using anti-COMT antibody (M00464). <br>
COMT was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-COMT Antibody (M00464) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-abcb5-picoband-trade-antibody-a02979-1-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02979-1-ABCB5-primary-antibodies-IHC-testing-1.jpgAnti-ABCB5 Antibody Picoband® IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979-1).
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ABCB5 was detected in paraffin-embedded section of human melanoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB5 Antibody (A02979-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02979-1-ABCB5-primary-antibodies-IHC-testing-2.jpgAnti-ABCB5 Antibody Picoband® IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979-1).
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ABCB5 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB5 Antibody (A02979-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02979-1-ABCB5-primary-antibodies-IHC-testing-3.jpgAnti-ABCB5 Antibody Picoband® IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979-1).
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ABCB5 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB5 Antibody (A02979-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-1-abcb5-primary-antibodies-ihc-testing-4.jpgAnti-ABCB5 Antibody Picoband®IHC analysis of ABCB5 using anti-ABCB5 antibody (A02979-1).<br>
ABCB5 was detected in a paraffin-embedded section of melanoma. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 5％ BSA. The tissue section was then incubated with rabbit anti-ABCB5 Antibody (A02979-1) at 5 μg/ml overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02979-1-ABCB5-primary-antibodies-FC-testing-4.jpgAnti-ABCB5 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ABCB5 antibody (A02979-1). <br> Overlay histogram showing HL-60 cells stained with A02979-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB5 Antibody (A02979-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-1-abcb5-primary-antibodies-if-testing-5.jpgAnti-ABCB5 Antibody Picoband® IF analysis of ABCB5 using anti-ABCB5 antibody (A02979-1). <br>
ABCB5 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ABCB5 Antibody (A02979-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02979-1-abcb5-primary-antibodies-wb-testing-6.jpgAnti-ABCB5 Antibody Picoband® Western blot analysis of ABCB5 using anti-ABCB5 antibody (A02979-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB5 antigen affinity purified polyclonal antibody (Catalog # A02979-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB5 at approximately 110KD. The expected band size for ABCB5 is at 110KD.
https://www.bosterbio.com/products/primary-antibodies/anti-aldh1a1-antibody-a01392-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-wb-testing-1.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br>
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.<br>
Lane 3: human HepG2 whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: rat liver tissue lysates,<br>
Lane 6: rat kidney tissue lysates,<br>
Lane 7: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified polyclonal antibody (Catalog # A01392) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01392-ALDH1A1-primary-antibodies-IHC-testing-2.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392).<br>
ALDH1A1 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01392-ALDH1A1-primary-antibodies-IHC-testing-3.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392).
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ALDH1A1 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-ihc-testing-4.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392).
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ALDH1A1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-ihc-testing-5.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392).
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ALDH1A1 was detected in paraffin-embedded section of mouse gaster tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01392-ALDH1A1-primary-antibodies-FC-testing-9.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®9. Flow Cytometry analysis of HL-60 cells using anti-ALDH1A1 antibody (A01392). <br> Overlay histogram showing HL-60 cells stained with A01392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (A01392,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-ihc-testing-6.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392).
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ALDH1A1 was detected in paraffin-embedded section of rat gaster tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-fc-testing-10.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband®10. Flow Cytometry analysis of HepG2 cells using anti-ALDH1A1 antibody (A01392). <br> Overlay histogram showing HepG2 cells stained with A01392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (A01392,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01392-ALDH1A1-primary-antibodies-IF-testing-7.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IF analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). <br> ALDH1A1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01392-aldh1a1-primary-antibodies-if-testing-8.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband® IF analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). <br> ALDH1A1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH1A1 Antibody (A01392) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-dcc-picoband-trade-antibody-a00560-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00560-DCC-primary-antibodies-WB-testing-7.jpgAnti-DCC Antibody Picoband® Western blot analysis of DCC using anti-DCC antibody (A00560).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human A431 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates.
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After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCC antigen affinity purified polyclonal antibody (Catalog # A00560) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCC at approximately 180KD. The expected band size for DCC is at 158KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00560-dcc-primary-antibodies-ihc-testing-5.jpgAnti-DCC Antibody Picoband®IHC analysis of DCC using anti-DCC antibody (A00560). <br>DCC was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00560-DCC-primary-antibodies-IHC-testing-1.jpgAnti-DCC Antibody Picoband® IHC analysis of DCC using anti-DCC antibody (A00560).
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DCC was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00560-DCC-primary-antibodies-IHC-testing-2.jpgAnti-DCC Antibody Picoband® IHC analysis of DCC using anti-DCC antibody (A00560).
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DCC was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00560-DCC-primary-antibodies-IHC-testing-3.jpgAnti-DCC Antibody Picoband® IHC analysis of DCC using anti-DCC antibody (A00560).
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DCC was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00560-DCC-primary-antibodies-IHC-testing-4.jpgAnti-DCC Antibody Picoband® IHC analysis of DCC using anti-DCC antibody (A00560).
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DCC was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DCC Antibody (A00560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-eif4a1-picoband-trade-antibody-a03922-2-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-ihc-testing-1.jpgAnti-EIF4A1/2/3 Antibody Picoband® IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
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EIF4A was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-wb-testing-10_1.jpgAnti-EIF4A1/2/3 Antibody Picoband® Western blot analysis of EIF4A(A1,2,3) using anti-EIF4A(A1,2,3) antibody (A03922-2 ). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human A431 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human A549 whole cell lysates, <br>
Lane 7: human U87 whole cell lysates, <br>
Lane 8: human K562 whole cell lysates,<br>
Lane 9: rat PC-12 whole cell lysates, <br>
Lane 10: mouse spleen tissue lysates, <br>
Lane 11: mouse thymus tissue lysates, <br>
Lane 12: mouse lung tissue lysates,<br>
Lane 13: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A(A1,2,3) antigen affinity purified polyclonal antibody (Catalog # A03922-2 ) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4A(A1,2,3) at approximately 46KD. The expected band size for EIF4A(A1,2,3) is at 46KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-ihc-testing-2.jpgAnti-EIF4A1/2/3 Antibody Picoband® IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
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EIF4A was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-ihc-testing-3.jpgAnti-EIF4A1/2/3 Antibody Picoband® IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
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EIF4A was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-ihc-testing-4.jpgAnti-EIF4A1/2/3 Antibody Picoband® IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
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EIF4A was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-ihc-testing-5.jpgAnti-EIF4A1/2/3 Antibody Picoband® IHC analysis of EIF4A using anti-EIF4A antibody (A03922-2).
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EIF4A was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-if-testing-6.jpgAnti-EIF4A1/2/3 Antibody Picoband® IF analysis of EIF4A using anti-EIF4A antibody (A03922-2). <br>
EIF4A was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-if-testing-7.jpgAnti-EIF4A1/2/3 Antibody Picoband® IF analysis of EIF4A using anti-EIF4A antibody (A03922-2). <br>
EIF4A was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EIF4A Antibody (A03922-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-fc-testing-8.jpgAnti-EIF4A1/2/3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-EIF4A antibody (A03922-2).<br>Overlay histogram showing HepG2 cells stained with A03922-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4A Antibody (A03922-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-2-eif4a-primary-antibodies-fc-testing-9.jpgAnti-EIF4A1/2/3 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-EIF4A antibody (A03922-2).<br>Overlay histogram showing U87 cells stained with A03922-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4A Antibody (A03922-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-eif4g1-picoband-trade-antibody-a01351-2-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IHC-testing-1.jpgAnti-EIF4G1 Antibody Picoband® IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2).
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EIF4G1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IHC-testing-2.jpgAnti-EIF4G1 Antibody Picoband® IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2).
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EIF4G1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IHC-testing-3.jpgAnti-EIF4G1 Antibody Picoband® IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2).
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EIF4G1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IHC-testing-4.jpgAnti-EIF4G1 Antibody Picoband® IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2).
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EIF4G1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IHC-testing-5.jpgAnti-EIF4G1 Antibody Picoband® IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2).
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EIF4G1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-IF-testing-6.jpgAnti-EIF4G1 Antibody Picoband® IF analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2). <br> EIF4G1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EIF4G1 Antibody (A01351-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-FC-testing-7.jpgAnti-EIF4G1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-EIF4G1 antibody (A01351-2). <br> Overlay histogram showing HepG2 cells stained with A01351-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4G1 Antibody (A01351-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01351-2-EIF4G1-primary-antibodies-WB-testing-8.jpgAnti-EIF4G1 Antibody Picoband® Western blot analysis of EIF4G1 using anti-EIF4G1 antibody (A01351-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human SW620 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4G1 antigen affinity purified polyclonal antibody (Catalog # A01351-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4G1 at approximately 250KD. The expected band size for EIF4G1 is at 175KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fibronectin-fn1-picoband-trade-antibody-a00564-1-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00564-1-fn1-primary-antibodies-wb-testing-1.jpgAnti-Fibronectin/FN1 Antibody Picoband® Western blot analysis of FN1 using anti-FN1 antibody (A00564-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br>
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 220-400 kDa. The expected band size for FN1 is at 272 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00564-1-fn1-primary-antibodies-fc-testing-4_1.jpgAnti-Fibronectin/FN1 Antibody Picoband®4. Flow Cytometry analysis of CACO-2 cells using anti-FN1 antibody (A00564-1). <br> Overlay histogram showing CACO-2 cells stained with A00564-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00564-1-fn1-primary-antibodies-ihc-testing-2.jpgAnti-Fibronectin/FN1 Antibody Picoband® IHC analysis of FN1 using anti-FN1 antibody (A00564-1). <br>
FN1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FN1 Antibody (A00564-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00564-1-fn1-primary-antibodies-fc-testing-5_1.jpgAnti-Fibronectin/FN1 Antibody Picoband®5. Flow Cytometry analysis of HeLa cells using anti-FN1 antibody (A00564-1). <br> Overlay histogram showing HeLa cells stained with A00564-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FN1 Antibody (A00564-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00564-1-fn1-primary-antibodies-ihc-testing-3.jpgAnti-Fibronectin/FN1 Antibody Picoband® IHC analysis of FN1 using anti-FN1 antibody (A00564-1). <br>
FN1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FN1 Antibody (A00564-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-connexin-43-gja1-picoband-trade-antibody-a00599-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-13046_2019_1142_fig1_html.pngAnti-Connexin 43/GJA1 Antibody Picoband®Expression and distribution of Cx32, Cx26 and Cx43 in patients with HCC. a. The protein expression levels of Cx32, Cx26 and Cx43 were determined by western blot analysis. β-actin was used as the loading control. b. The expression of Cx32 was correlated with increased TNM stages, as revealed by western blot analysis. β-actin was used as the loading control. c . Statistical analysis of the relative expression levels of Cxs in HCC tissues, peritumoral tissues, and normal liver tissues. ** , P < 0.01; ## , P < 0.01. d. Statistical analysis of the relative expression levels of Cx32 in peritumoral tissues and HCC tissues with different TNM stages. * , P < 0.05. e. Representative IHC staining of Cx32, Cx26 and Cx43 protein in normal liver tissues (left panels), peritumoral tissues (middle panels) and HCC tissues (right panels) (400×). Scale bars: 50 μm. f. Representative IHC staining of Cx32 in normal liver tissues, cirrhotic tissues and early and advanced HCC tissues (400×). Scale bars: 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-fphar-10-00175-g004.jpgAnti-Connexin 43/GJA1 Antibody Picoband®Trimetazidine suppressed EE-induced change in the expression of connexin 43 (CX43) in myocardial tissues of rats. The mRNA and protein level of CX43 was determined by real-time PCR (A) , and western blot (B) . The expression and distribution of CX43 in myocardial tissues were observed by immunofluorescence staining (C) . Scale bar is 50 μm. Each value is shown as mean ± SD ( n = 6). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, versus the indicated group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00175/full'>30890937</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-gja1-primary-antibodies-wb-testing-1_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband®Western blot analysis of GJA1 using anti-GJA1 antibody (A00599). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human U251 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse heart tissue lysates,<br>
Lane 7: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA1 antigen affinity purified polyclonal antibody (A00599) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43 kDa. The expected band size for GJA1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-gja1-primary-antibodies-ihc-testing-1.jpgAnti-Connexin 43/GJA1 Antibody Picoband®IHC analysis of GJA1 using anti-GJA1 antibody (A00599). <br>GJA1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (A00599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-gja1-primary-antibodies-ihc-testing-2_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband®IHC analysis of GJA1 using anti-GJA1 antibody (A00599). <br>GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (A00599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-gja1-primary-antibodies-ihc-testing-3_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband®IHC analysis of GJA1 using anti-GJA1 antibody (A00599). <br>GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (A00599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00599-gja1-primary-antibodies-ihc-testing-4_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband®IHC analysis of GJA1 using anti-GJA1 antibody (A00599). <br>GJA1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (A00599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-glypican-3-gpc3-picoband-trade-antibody-a01922-2-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01922-2-gpc3-primary-antibodies-wb-testing-1.jpgAnti-Glypican 3/GPC3 Antibody Picoband® Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01922-2-gpc3-primary-antibodies-fcm-testing-2.jpgAnti-Glypican 3/GPC3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Glypican 3/GPC3 antibody (A01922-2). <br>Overlay histogram showing HepG2 cells stained with A01922-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Glypican 3/GPC3 Antibody (A01922-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hexokinase-1-hk1-picoband-trade-antibody-a01504-1-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-wb-testing-1.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® Western blot analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human U20S whole cell lysates, <br>
Lane 3: human Hek293 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat heart tissue lysates, <br>
Lane 7: rat lung tissue lysates, <br>
Lane 8: mouse brain tissue lysates, <br>
Lane 9: mouse heart tissue lysates, <br>
Lane 10: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hexokinase 1/HK1 antigen affinity purified polyclonal antibody (Catalog # A01504-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hexokinase 1/HK1 at approximately 102KD. The expected band size for Hexokinase 1/HK1 is at 102KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-ihc-testing-2.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-ihc-testing-3.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-ihc-testing-4.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-ihc-testing-5.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01504-1-hk1-primary-antibodies-if-testing-6.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® IF analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (A01504-1). <br>
Hexokinase 1/HK1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hexokinase 1/HK1 Antibody (A01504-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ifnar2-picoband-trade-antibody-a02056-3-boster.html2026-03-24T05:25:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-IHC-testing-1.jpgAnti-IFNAR2 Antibody Picoband® IHC analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3).
<br>
IFNAR2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNAR2 Antibody (A02056-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-IHC-testing-2.jpgAnti-IFNAR2 Antibody Picoband® IHC analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3).
<br>
IFNAR2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNAR2 Antibody (A02056-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-IHC-testing-3.jpgAnti-IFNAR2 Antibody Picoband® IHC analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3).
<br>
IFNAR2 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNAR2 Antibody (A02056-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-IHC-testing-4.jpgAnti-IFNAR2 Antibody Picoband® IHC analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3).
<br>
IFNAR2 was detected in paraffin-embedded section of rat ntestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNAR2 Antibody (A02056-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-IF-testing-5.jpgAnti-IFNAR2 Antibody Picoband® IF analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3). <br> IFNAR2 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IFNAR2 Antibody (A02056-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-FC-testing-6.jpgAnti-IFNAR2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-IFNAR2 antibody (A02056-3). <br> Overlay histogram showing A549 cells stained with A02056-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFNAR2 Antibody (A02056-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02056-3-IFNAR2-primary-antibodies-WB-testing-7.jpgAnti-IFNAR2 Antibody Picoband® Western blot analysis of IFNAR2 using anti-IFNAR2 antibody (A02056-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNAR2 antigen affinity purified polyclonal antibody (Catalog # A02056-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNAR2 at approximately 65KD. The expected band size for IFNAR2 is at 58-65KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mmp1-picoband-trade-antibody-a00733-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00733-1-mmp1-primary-antibodies-wb-testing-1_1_1.jpgAnti-MMP1 Antibody Picoband®Western blot analysis of MMP1 using anti-MMP1 antibody (A00733-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human U2OS whole cell lysates,<br>
Lane 5: human MCF-7 whole cell lysates,<br>
Lane 6: rat RH35 whole cell lysates,<br>
Lane 7: mouse HEPA1-6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (A00733-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54 kDa. The expected band size for MMP1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00733-1-mmp1-primary-antibodies-ihc-testing-2.jpgAnti-MMP1 Antibody Picoband® IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
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MMP1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00733-1-mmp1-primary-antibodies-ihc-testing-3.jpgAnti-MMP1 Antibody Picoband® IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
<br>
MMP1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00733-1-MMP1-primary-antibodies-IHC-testing-4.jpgAnti-MMP1 Antibody Picoband® IHC analysis of MMP1 using anti-MMP1 antibody (A00733-1).
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MMP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00733-1-mmp1-primary-antibodies-if-testing-5.jpgAnti-MMP1 Antibody Picoband® IF analysis of MMP1 using anti-MMP1 antibody (A00733-1). <br>
MMP1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MMP1 Antibody (A00733-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00733-1-mmp1-primary-antibodies-fcm-testing-6.jpgAnti-MMP1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-MMP1 antibody (A00733-1). <br>Overlay histogram showing PC-3 cells stained with A00733-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP1 Antibody (A00733-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mttp-picoband-trade-antibody-a01715-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01715-1-mttp-primary-antibodies-wb-testing-1.jpgAnti-MTTP Antibody Picoband® Western blot analysis of MTTP using anti-MTTP antibody (A01715-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEPG2 whole cell lysates, <br>
Lane 2: monkey liver tissue lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: rat small intestine tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTTP antigen affinity purified polyclonal antibody (Catalog # A01715-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTTP at approximately 99KD. The expected band size for MTTP is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01715-1-mttp-primary-antibodies-ihc-testing-2.jpgAnti-MTTP Antibody Picoband® IHC analysis of MTTP using anti-MTTP antibody (A01715-1). <br>
MTTP was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MTTP Antibody (A01715-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01715-1-mttp-primary-antibodies-ihc-testing-3.jpgAnti-MTTP Antibody Picoband® IHC analysis of MTTP using anti-MTTP antibody (A01715-1). <br>
MTTP was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MTTP Antibody (A01715-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01715-1-mttp-primary-antibodies-if-testing-4.jpgAnti-MTTP Antibody Picoband® IF analysis of MTTP using anti-MTTP antibody (A01715-1). <br>
MTTP was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MTTP Antibody (A01715-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01715-1-mttp-primary-antibodies-fcm-testing-5.pngAnti-MTTP Antibody Picoband® Flow Cytometry analysis of HEPG2 cells using anti-MTTP antibody (A01715-1). <br>
Overlay histogram showing HEPG2 cells stained with A01715-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTTP Antibody (A01715-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-nucleolin-ncl-picoband-trade-antibody-a00228-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-wb-testing-1.jpgAnti-Nucleolin/NCL Antibody Picoband® Western blot analysis of NCL using anti-NCL antibody (A00228-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: monkey COS-7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCL antigen affinity purified polyclonal antibody (Catalog # A00228-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCL at approximately 100-110 kDa. The expected band size for NCL is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-ihc-testing-2.jpgAnti-Nucleolin/NCL Antibody Picoband® IHC analysis of NCL using anti-NCL antibody (A00228-1). <br>
NCL was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCL Antibody (A00228-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g001.jpgAnti-Nucleolin/NCL Antibody Picoband®Annotation Results of scRNA-seq for LUAD. (A) Sample origin of the single-cell data, 12 samples were identified without batch effect. (B) Transcript counts in the single-cell dataset. (C) Clustering results of the single-cell data, totally 21 clusters were presented. (D) Cell type annotation based on marker gene expression, including T cells, monocyte-macrophages, NK cells, epithelial cells, B cells, fibroblasts, mast cells, endothelial cells, conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs). (E) Expression profiles of representative markers for ten distinct cell types. (F) Proportion of each cell type across samples. (G) Total number of cells for each identified cell type. (H) Transcript counts per cell type, reflecting transcriptional activity at the single-cell level.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g003.jpgAnti-Nucleolin/NCL Antibody Picoband®Single-cell communication networks. (A) Incoming communication patterns of target cells, showing pathways to which each cell type responds. (B) Outgoing communication patterns of secreting cells, illustrating the pathways through which cells send signals, MIF, MK and CXCL pathway exhibit high activity. (C) Network diagram showing the strength of intercellular communication, with connections between various cell types. (D) Scatter plot comparing outgoing and incoming communication strengths across cell populations, with bubble size indicating the number of interactions, malignant cells have higher strength of intercellular communication. (E) Chord diagram depicting communication via the MK pathway between different cell types. (F) Ligand-receptor interaction probabilities within the MK pathway between malignant and other cell types. Dot size represents significance (P-value), and color represents communication probability highlighting the MDK-NCL signaling pathway. (G) Violin plots of MK pathway gene expression levels across cell types, showing gene activity variations, MDK has advancer expression level in malignant cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g004.jpgAnti-Nucleolin/NCL Antibody Picoband®Spatial transcriptomics and MDK-NCL signal communication. (A) Niche clustering in spatial transcriptomics samples, identifying distinct ecological zones. (B) Spatial expression of representative markers in key regions: MUC1 (tumor region), LYZ (immune region), COL14A1 (stromal region), and SFTPC (normal region). (C) Violin plots displaying the expression of MUC1, LYZ, COL14A1, and SFTPC across different niches. (D) MCPcounter analysis showing the infiltration of six cell types (e.g., endothelial cells, fibroblasts, immune lineages) across spatial regions. (E) Spatial niche classification, distinguishing tumor, immune-stromal, and normal regions. (F) MDK-NCL ligand-receptor interaction analysis, spatially mapping MDK ligands, NCL receptors, and their binding regions.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g005.jpgAnti-Nucleolin/NCL Antibody Picoband®Single-cell pseudotime analysis. (A) Pseudotime trajectory analysis showing the 6 differentiation states of cells. (B) Subtype classification of malignant cells along the pseudotime trajectory. (C) Pseudotime scores mapped along the differentiation trajectory. (D) UMAP plot visualizing pseudotime scores across individual cells. (E) Box plots comparing pseudotime scores across different malignant cell clusters, cluster 0, 1, and 5 had higher pseudotime scores. (F) UMAP plot of differentiation states, with colors representing distinct states. (G) Stacked bar plots showing the proportion of differentiation states within each malignant cell cluster, cluster 0, 1, and 5 have larger proportion of state 6. (H) Expression dynamics of MK pathway genes (e.g., MDK, NCL, ITG genes) along the pseudotime trajectory, highlighting gene expression changes during differentiation, MDK and NCL express more in the later time.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g006.jpgAnti-Nucleolin/NCL Antibody Picoband®Association of MDK-NCL with the immune microenvironment. (A) Boxplot shows the expression levels of MDK and NCL genes in tumor and control groups, it exhibit higher activity in tumor group. (B) MDK-NCL enrichment scores in tumor and control groups. (C) Relative mRNA expression levels of MDK and NCL in tumor and control groups from in-house data. (D) Relative protein expression levels of MDK and NCL in tumor and control groups from in-house data. (E) Comparison of MDK protein expression levels between tumor and control groups. (F) Comparison of NCL protein expression levels between tumor and control groups. (G) Correlation of MDK and NCL expression with ImmuneScore, StromalScore, ESTIMATEScore, and TumorPurity. (H) Scatter plots depicting the relationship between MDK and NCL expression and immune-related scores (ImmuneScore, StromalScore, ESTIMATEScore) as well as TumorPurity. (I) Comparison of immune cell infiltration scores across high and low MDK-NCL expression groups for 28 immune cell types. *P < 0.05, **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-fimmu-16-1546382-g007.jpgAnti-Nucleolin/NCL Antibody Picoband®Association of MDK-NCL with immunotherapy response. (A) Comparison of tumor mutation burden (TMB) between high and low MDK-NCL expression groups. (B) Comparison of microsatellite instability (MSI) between high and low MDK-NCL groups. (C) Comparison of dysfunction scores between high and low MDK-NCL groups. (D) Comparison of exclusion scores between high and low MDK-NCL groups. (E) Expression of immunogenic cell death (ICD)-related genes in high and low MDK-NCL groups. (F) Expression levels of CTLA4 and PD1 in high and low MDK-NCL groups. (G) Comparison of immune checkpoint gene expression between high and low MDK-NCL expression groups. *P < 0.05, **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12089103/'>40396179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-ihc-testing-3.jpgAnti-Nucleolin/NCL Antibody Picoband® IHC analysis of NCL using anti-NCL antibody (A00228-1). <br>
NCL was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCL Antibody (A00228-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-ihc-testing-4.jpgAnti-Nucleolin/NCL Antibody Picoband® IHC analysis of NCL using anti-NCL antibody (A00228-1). <br>
NCL was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCL Antibody (A00228-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-ihc-testing-5.jpgAnti-Nucleolin/NCL Antibody Picoband® IF analysis of NCL using anti-NCL antibody (A00228-1) and anti-Tubulin beta antibody (M05613-4). <br>
NCL was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NCL Antibody (A00228-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00228-1-ncl-primary-antibodies-fcm-testing-6_1.jpgAnti-Nucleolin/NCL Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-NCL antibody (A00228-1). <br>Overlay histogram showing HL-60 cells stained with A00228-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCL Antibody (A00228-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nf2-merlin-picoband-trade-antibody-a00279-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-wb-testing-1.jpgAnti-NF2/Merlin Antibody Picoband® Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human U251 whole cell lysates,<br>
Lane 4: rat kidney tissue lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse HEPA1-6 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-fc-testing-7.jpgAnti-NF2/Merlin Antibody Picoband®7. Flow Cytometry analysis of A549 cells using anti-NF2 antibody (A00279-2). <br> Overlay histogram showing A549 cells stained with A00279-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF2 Antibody (A00279-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-fc-testing-8.jpgAnti-NF2/Merlin Antibody Picoband®8. Flow Cytometry analysis of HL-60 cells using anti-NF2 antibody (A00279-2). <br> Overlay histogram showing HL-60 cells stained with A00279-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF2 Antibody (A00279-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-ihc-testing-2.jpgAnti-NF2/Merlin Antibody Picoband® IHC analysis of NF2 using anti-NF2 antibody (A00279-2).
<br>
NF2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NF2 Antibody (A00279-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-ihc-testing-3_1.jpgAnti-NF2/Merlin Antibody Picoband® IHC analysis of NF2 using anti-NF2 antibody (A00279-2).
<br>
NF2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NF2 Antibody (A00279-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-ihc-testing-4.jpgAnti-NF2/Merlin Antibody Picoband® IHC analysis of NF2 using anti-NF2 antibody (A00279-2).
<br>
NF2 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NF2 Antibody (A00279-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-ihc-testing-5.jpgAnti-NF2/Merlin Antibody Picoband® IHC analysis of NF2 using anti-NF2 antibody (A00279-2).
<br>
NF2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NF2 Antibody (A00279-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00279-2-nf2-primary-antibodies-if-testing-6.jpgAnti-NF2/Merlin Antibody Picoband® IF analysis of NF2 using anti-NF2 antibody (A00279-2) <br> NF2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NF2 Antibody (A00279-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-nfat2-nfatc1-picoband-trade-antibody-a00340-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00340-2-nfatc1-primary-antibodies-wb-testing-1.jpgAnti-NFAT2/NFATC1 Antibody Picoband® Western blot analysis of NFAT2/NFATC1 using anti-NFAT2/NFATC1 antibody (A00340-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates, <br>
Lane 2: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2/NFATC1 antigen affinity purified polyclonal antibody (Catalog # A00340-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT2/NFATC1 at approximately 120KD. The expected band size for NFAT2/NFATC1 is at 101KD.
https://www.bosterbio.com/products/primary-antibodies/anti-notch2-picoband-trade-antibody-a00518-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-wb-testing-1.jpgAnti-NOTCH2 Antibody Picoband® Western blot analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: human SH-SY5Y whole cell lysates,<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH2 antigen affinity purified polyclonal antibody (Catalog # A00518-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOTCH2 at approximately 110 kDa, 300 kDa. The expected band size for NOTCH2 is at 265 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-ihc-testing-2.jpgAnti-NOTCH2 Antibody Picoband® IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
NOTCH2 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOTCH2 Antibody (A00518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-ihc-testing-3.jpgAnti-NOTCH2 Antibody Picoband® IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
NOTCH2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOTCH2 Antibody (A00518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-ihc-testing-4.jpgAnti-NOTCH2 Antibody Picoband® IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
NOTCH2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOTCH2 Antibody (A00518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-ihc-testing-5.jpgAnti-NOTCH2 Antibody Picoband® IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
NOTCH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOTCH2 Antibody (A00518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-ihc-testing-6.jpgAnti-NOTCH2 Antibody Picoband® IHC analysis of NOTCH2 using anti-NOTCH2 antibody (A00518-2). <br>
NOTCH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOTCH2 Antibody (A00518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00518-2-notch2-primary-antibodies-fcm-testing-7.jpgAnti-NOTCH2 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-NOTCH2 antibody (A00518-2). <br>
Overlay histogram showing SH-SY5Y cells stained with A00518-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOTCH2 Antibody (A00518-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-orai1-antibody-a00909-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00909-orai1-primary-antibodies-wb-testing-1.jpgAnti-Orai1 Antibody Picoband® Western blot analysis of ORAI1 using anti-ORAI1 antibody (A00909). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A375 whole cell lysates, <br> Lane 2: human Jurka whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORAI1 antigen affinity purified polyclonal antibody (Catalog # A00909) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORAI1 at approximately 39KD. The expected band size for ORAI1 is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00909-orai1-primary-antibodies-fc-testing-2.pngAnti-Orai1 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-ORAI1 antibody (A00909). <br> Overlay histogram showing CACO-2 cells stained with A00909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORAI1 Antibody (A00909,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00909-orai1-primary-antibodies-fc-testing-3.pngAnti-Orai1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ORAI1 antibody (A00909). <br> Overlay histogram showing A549 cells stained with A00909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORAI1 Antibody (A00909,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-oct4-pou5f1-antibody-a00174-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00174-1-pou5f1-primary-antibodies-wb-testing-1.jpgAnti-Oct4/POU5F1 Antibody Picoband® Western blot analysis of POU5F1 using anti-POU5F1 antibody (A00174-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POU5F1 at approximately 45 kDa. The expected band size for POU5F1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00174-1-pou5f1-primary-antibodies-fcm-testing-2.jpgAnti-Oct4/POU5F1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-POU5F1 antibody (A00174-1). <br>
Overlay histogram showing A431 cells stained with A00174-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (A00174-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pkc-delta-prkcd-picoband-trade-antibody-a00822-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-1.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00822-1-prkcd-primary-antibodies-wb-testing-10_1_1.jpgAnti-PKC delta/PRKCD Antibody Picoband® Western blot analysis of PRKCD using anti-PRKCD antibody (A00822-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain issue lysates, <br> Lane 2: mouse brain issue lysates, <br> Lane 3: mouse thymus issue lysates, <br> Lane 4: mouse lung issue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCD antigen affinity purified polyclonal antibody (Catalog # A00822-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCD at approximately 77KD. The expected band size for PRKCD is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-2.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-3.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-4.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-5.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IHC-testing-6.jpgAnti-PKC delta/PRKCD Antibody Picoband® IHC analysis of PRKCD using anti-PRKCD antibody (A00822-1).
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PRKCD was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-IF-testing-7.jpgAnti-PKC delta/PRKCD Antibody Picoband® IF analysis of PRKCD using anti-PRKCD antibody (A00822-1). <br> PRKCD was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-FC-testing-8.jpgAnti-PKC delta/PRKCD Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-PRKCD antibody (A00822-1). <br> Overlay histogram showing CACO-2 cells stained with A00822-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKCD Antibody (A00822-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00822-1-PRKCD-primary-antibodies-FC-testing-9.jpgAnti-PKC delta/PRKCD Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PRKCD antibody (A00822-1). <br> Overlay histogram showing THP-1 cells stained with A00822-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKCD Antibody (A00822-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00822-1-prkcd-primary-antibodies-wb-testing-11.jpgAnti-PKC delta/PRKCD Antibody Picoband® Western blot analysis of PRKCD using anti-PRKCD antibody (A00822-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta issue lysates, <br> Lane 2: human THP-1 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human HEK293 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCD antigen affinity purified polyclonal antibody (Catalog # A00822-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCD at approximately 77KD. The expected band size for PRKCD is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00822-1-prkcd-primary-antibodies-if-testing-12.jpgAnti-PKC delta/PRKCD Antibody Picoband® IF analysis of PRKCD using anti-PRKCD antibody (A00822-1). <br>
PRKCD was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PRKCD Antibody (A00822-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ptbp1-picoband-trade-antibody-a01798-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-wb-testing-1.jpgAnti-PTBP1 Antibody Picoband® Western blot analysis of PTBP1 using anti-PTBP1 antibody (A01798). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTBP1 antigen affinity purified polyclonal antibody (Catalog # A01798) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTBP1 at approximately 57 kDa. The expected band size for PTBP1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-2.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-3.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-4.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-5.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-6.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-ihc-testing-7.jpgAnti-PTBP1 Antibody Picoband® IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
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PTBP1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-if-testing-8.jpgAnti-PTBP1 Antibody Picoband® IF analysis of PTBP1 using anti-PTBP1 antibody (A01798). <br>
PTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PTBP1 Antibody (A01798) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-fc-testing-9.jpgAnti-PTBP1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PTBP1 antibody (A01798).<br>Overlay histogram showing 293T cells stained with A01798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTBP1 Antibody (A01798,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01798-ptbp1-primary-antibodies-wb-testing-10.jpgAnti-PTBP1 Antibody Picoband® Western blot analysis of PTBP1 using anti-PTBP1 antibody (A01798). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates, <br>
Lane 2: human RT4 whole cell lysates, <br>
Lane 3: human SiHa whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTBP1 antigen affinity purified polyclonal antibody (A01798) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PTBP1 at approximately 57 kDa. The expected band size for PTBP1 is at 57 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-ptbp2-picoband-trade-antibody-a05020-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-IHC-testing-1.jpgAnti-PTBP2 Antibody Picoband® IHC analysis of PTBP2 using anti-PTBP2 antibody (A05020-2).
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PTBP2 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP2 Antibody (A05020-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-IHC-testing-2.jpgAnti-PTBP2 Antibody Picoband® IHC analysis of PTBP2 using anti-PTBP2 antibody (A05020-2).
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PTBP2 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP2 Antibody (A05020-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-IHC-testing-3.jpgAnti-PTBP2 Antibody Picoband® IHC analysis of PTBP2 using anti-PTBP2 antibody (A05020-2).
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PTBP2 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP2 Antibody (A05020-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-IHC-testing-4.jpgAnti-PTBP2 Antibody Picoband® IHC analysis of PTBP2 using anti-PTBP2 antibody (A05020-2).
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PTBP2 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTBP2 Antibody (A05020-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-IF-testing-5.jpgAnti-PTBP2 Antibody Picoband® IF analysis of PTBP2 using anti-PTBP2 antibody (A05020-2). <br> PTBP2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PTBP2 Antibody (A05020-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-FC-testing-6.jpgAnti-PTBP2 Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-PTBP2 antibody (A05020-2). <br> Overlay histogram showing HeLa cells stained with A05020-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTBP2 Antibody (A05020-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-FC-testing-7.jpgAnti-PTBP2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PTBP2 antibody (A05020-2). <br> Overlay histogram showing THP-1 cells stained with A05020-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTBP2 Antibody (A05020-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-WB-testing-8.jpgAnti-PTBP2 Antibody Picoband® Western blot analysis of PTBP2 using anti-PTBP2 antibody (A05020-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human MDA-MB-453 whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human U-937 whole cell lysates, <br>
Lane 6: human HEK293 whole cell lysates, <br>
Lane 7: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTBP2 antigen affinity purified polyclonal antibody (Catalog # A05020-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTBP2 at approximately 57KD. The expected band size for PTBP2 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05020-2-PTBP2-primary-antibodies-WB-testing-9.jpgAnti-PTBP2 Antibody Picoband® Western blot analysis of PTBP2 using anti-PTBP2 antibody (A05020-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular issue lysates, <br>
Lane 2: mouse testicular issue lysates, <br>
Lane 3: mouse thymus issue lysates, <br>
Lane 4: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTBP2 antigen affinity purified polyclonal antibody (Catalog # A05020-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTBP2 at approximately 57KD. The expected band size for PTBP2 is at 57KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fox2-rbm9-rbfox2-picoband-trade-antibody-a05389-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-wb-testing-1.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® Western blot analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates,<br>
Lane 2: human Caco-2 whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human U87 whole cell lysates,<br>
Lane 5: rat C6 whole cell lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBFOX2 antigen affinity purified polyclonal antibody (A05389-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBFOX2 at approximately 60 kDa. The expected band size for RBFOX2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-ihc-testing-2_1.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>RBFOX2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-ihc-testing-3_1.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>RBFOX2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-ihc-testing-4_1.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>RBFOX2 was detected in a paraffin-embedded section of rat brain cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-ihc-testing-5.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>RBFOX2 was detected in a paraffin-embedded section of rat hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-if-testing-6.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IF analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>
RBFOX2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-if-testing-7.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IF analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1). <br>
RBFOX2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBFOX2 Antibody (A05389-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-1-rbfox2-primary-antibodies-if-testing-8.jpgAnti-Fox2/RBM9/RBFOX2 Antibody Picoband® IF analysis of RBFOX2 using anti-RBFOX2 antibody (A05389-1) and anti-Tubulin Alpha antibody (M03989-3).<br>
RBFOX2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBFOX2 Antibody (A05389-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-sirt1-picoband-trade-antibody-a00018-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00018-1-sirt1-primary-antibodies-wb-testing-1.jpgAnti-SIRT1 Antibody Picoband® Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (Catalog # A00018-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00018-1-sirt1-primary-antibodies-wb-testing-2.jpgAnti-SIRT1 Antibody Picoband® Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SiHa whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (Catalog # A00018-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00018-1-sirt1-primary-antibodies-if-testing-3.jpgAnti-SIRT1 Antibody Picoband® IF analysis of SIRT1 using anti-SIRT1 antibody (A00018-1) and anti-Beta Tubulin antibody (M01857-3).<br>
SIRT1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIRT1 Antibody (A00018-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-smarca2-brm-picoband-trade-antibody-a01888-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01888-2-SMARCA2-primary-antibodies-FC-testing-1.jpgAnti-SMARCA2/BRM Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-SMARCA2 antibody (A01888-2). <br> Overlay histogram showing HeLa cells stained with A01888-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA2 Antibody (A01888-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01888-2-SMARCA2-primary-antibodies-FC-testing-2.jpgAnti-SMARCA2/BRM Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SMARCA2 antibody (A01888-2). <br> Overlay histogram showing THP-1 cells stained with A01888-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA2 Antibody (A01888-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01888-2-SMARCA2-primary-antibodies-WB-testing-3.jpgAnti-SMARCA2/BRM Antibody Picoband® Western blot analysis of SMARCA2 using anti-SMARCA2 antibody (A01888-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular issue lysates, <br>
Lane 2: mouse testicula issue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA2 antigen affinity purified polyclonal antibody (Catalog # A01888-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCA2 at approximately 210KD. The expected band size for SMARCA2 is at 181KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01888-2-SMARCA2-primary-antibodies-WB-testing-4.jpgAnti-SMARCA2/BRM Antibody Picoband® Western blot analysis of SMARCA2 using anti-SMARCA2 antibody (A01888-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates, <br>
Lane 2: human THP-1 whole cell lysates, <br>
Lane 3: human A549whole cell lysates, <br>
Lane 4: human U2OS whole cell lysates, <br>
Lane 5: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA2 antigen affinity purified polyclonal antibody (Catalog # A01888-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCA2 at approximately 210KD. The expected band size for SMARCA2 is at 181KD.
https://www.bosterbio.com/products/primary-antibodies/anti-smox-picoband-trade-antibody-a05519-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05519-1-SMOX-primary-antibodies-WB-testing-1.jpgAnti-SMOX Antibody Picoband® Western blot analysis of SMOX using anti-SMOX antibody (A05519-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05519-1-SMOX-primary-antibodies-WB-testing-2.jpgAnti-SMOX Antibody Picoband® Western blot analysis of SMOX using anti-SMOX antibody (A05519-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain issue lysates, <br>
Lane 2: rat heart issue lysates, <br>
Lane 3: mouse brain issue lysates, <br>
Lane 4: mouse NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-1-smox-primary-antibodies-fcm-testing-3.pngAnti-SMOX Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SMOX antibody (A05519-1). <br>Overlay histogram showing PC-3 cells stained with A05519-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOX Antibody (A05519-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-smurf2-picoband-trade-antibody-a02585-1-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02585-1-SMURF2-primary-antibodies-WB-testing-1.jpgAnti-SMURF2 Antibody Picoband® Western blot analysis of SMURF2 using anti-SMURF2 antibody (A02585-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # A02585-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02585-1-SMURF2-primary-antibodies-WB-testing-2.jpgAnti-SMURF2 Antibody Picoband® Western blot analysis of SMURF2 using anti-SMURF2 antibody (A02585-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular issue lysates, <br>
Lane 2: rat pancreas issue lysates, <br>
Lane 3: rat stomach issue lysates, <br>
Lane 4: mouse testicular issue lysates, <br>
Lane 5: mouse pancreas issue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # A02585-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.
https://www.bosterbio.com/products/primary-antibodies/anti-syk-picoband-trade-antibody-a00490-3-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-wb-testing-1.jpgAnti-SYK Antibody Picoband®Western blot analysis of SYK using anti-SYK antibody (A00490-3). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human RT4 whole cell lysates,<br>
Lane 5: rat spleen tissue lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYK antigen affinity purified polyclonal antibody (A00490-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYK at approximately 72 kDa. The expected band size for SYK is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-wb-testing-2.pngAnti-SYK Antibody Picoband®Western blot analysis of SYK using anti-SYK antibody (A00490-3). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse HT22 whole cell lysates,<br>
Lane 2: low concentration drug treamented-mouse HT22 whole cell lysates,<br>
Lane 3: medium concentration drug treamented-mouse HT22 whole cell lysates,<br>
Lane 4: High concentration drug treamented-mouse HT22 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYK antigen affinity purified polyclonal antibody (A00490-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYK at approximately 72 kDa. The expected band size for SYK is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-ihc-testing-1.jpgAnti-SYK Antibody Picoband®IHC analysis of SYK using anti-SYK antibody (A00490-3). <br>SYK was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYK Antibody (A00490-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-ihc-testing-2.jpgAnti-SYK Antibody Picoband®IHC analysis of SYK using anti-SYK antibody (A00490-3). <br>SYK was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYK Antibody (A00490-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-ihc-testing-3.jpgAnti-SYK Antibody Picoband®IHC analysis of SYK using anti-SYK antibody (A00490-3). <br>SYK was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYK Antibody (A00490-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00490-3-syk-primary-antibodies-fcm-testing-1.jpgAnti-SYK Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SYK antibody (A00490-3). <br>
Overlay histogram showing RT4 cells stained with A00490-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tie2-tek-picoband-trade-antibody-a01274-2-boster.html2026-03-24T05:25:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-1.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
<br>
TEK was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-WB-testing-10.jpgAnti-TIE2/TEK Antibody Picoband® Western blot analysis of TEK using anti-TEK antibody (A01274-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse lung tissue lysates, <br>
Lane 2: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-2.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
<br>
TEK was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-3.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
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TEK was detected in paraffin-embedded section of human ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-4.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
<br>
TEK was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-5.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
<br>
TEK was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IHC-testing-6.jpgAnti-TIE2/TEK Antibody Picoband® IHC analysis of TEK using anti-TEK antibody (A01274-2).
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TEK was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-IF-testing-7.jpgAnti-TIE2/TEK Antibody Picoband® IF analysis of TEK using anti-TEK antibody (A01274-2). <br> TEK was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TEK Antibody (A01274-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-FC-testing-8.jpgAnti-TIE2/TEK Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-TEK antibody (A01274-2). <br> Overlay histogram showing HeLa cells stained with A01274-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TEK Antibody (A01274-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01274-2-TEK-primary-antibodies-WB-testing-9.jpgAnti-TIE2/TEK Antibody Picoband® Western blot analysis of TEK using anti-TEK antibody (A01274-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: human HL-60 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.
https://www.bosterbio.com/products/primary-antibodies/anti-baff-tnfsf13b-picoband-trade-antibody-a01257-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01257-TNFSF13B-primary-antibodies-FC-testing-1.jpgAnti-BAFF/TNFSF13B Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-TNFSF13B antibody (A01257). <br> Overlay histogram showing HL-60 cells stained with A01257 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFSF13B Antibody (A01257,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01257-TNFSF13B-primary-antibodies-FC-testing-2.jpgAnti-BAFF/TNFSF13B Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TNFSF13B antibody (A01257). <br> Overlay histogram showing U87 cells stained with A01257 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFSF13B Antibody (A01257,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01257-TNFSF13B-primary-antibodies-WB-testing-3.jpgAnti-BAFF/TNFSF13B Antibody Picoband® Western blot analysis of TNFSF13B using anti-TNFSF13B antibody (A01257). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U-937 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human PANC-1 whole cell lysates, <br>
Lane 4: human CCRF-CEM whole cell lysates, <br>
Lane 5: human MDA-MB-231 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF13B antigen affinity purified polyclonal antibody (Catalog # A01257) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFSF13B at approximately 34KD. The expected band size for TNFSF13B is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01257-TNFSF13B-primary-antibodies-WB-testing-4.jpgAnti-BAFF/TNFSF13B Antibody Picoband® Western blot analysis of TNFSF13B using anti-TNFSF13B antibody (A01257). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testicular issue lysates, <br>
Lane 2: mouse testicular issue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF13B antigen affinity purified polyclonal antibody (Catalog # A01257) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFSF13B at approximately 34KD. The expected band size for TNFSF13B is at 34KD.
https://www.bosterbio.com/products/primary-antibodies/anti-troponin-i-fast-skeletal-muscle-tnni2-picoband-trade-antibody-a07355-2-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-wb-testing-1.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® Western blot analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates, <br> Lane 2: mouse skeletal muscle tissue lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI2 antigen affinity purified polyclonal antibody (Catalog # A07355-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI2 at approximately 24KD. The expected band size for TNNI2 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-ihc-testing-2.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® IHC analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br> TNNI2 was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNNI2 Antibody (A07355-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-ihc-testing-3.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® IHC analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br> TNNI2 was detected in paraffin-embedded section of mouse skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNNI2 Antibody (A07355-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-ihc-testing-4.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® IHC analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br> TNNI2 was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNNI2 Antibody (A07355-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-if-testing-5.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® IF analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br>
TNNI2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TNNI2 Antibody (A07355-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07355-2-tnni2-primary-antibodies-if-testing-6.jpgAnti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® IF analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). <br>
TNNI2 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TNNI2 Antibody (A07355-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-tnnt3-picoband-trade-antibody-a05885-2-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05885-2-tnnt3-primary-antibodies-wb-testing-1.jpgAnti-TNNT3 Antibody Picoband®Western blot analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: rat skeletal muscle issue lysates,<br>
Lane 2: rat skeletal muscle issue lysates,<br>
Lane 3: mouse skeletal muscle issue lysates,<br>
Lane 4: mouse skeletal muscle issue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNT3 antigen affinity purified polyclonal antibody (A05885-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNNT3 at approximately 37 kDa. The expected band size for TNNT3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05885-2-tnnt3-primary-antibodies-ihc-testing-1.jpgAnti-TNNT3 Antibody Picoband®IHC analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). <br>TNNT3 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNT3 Antibody (A05885-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05885-2-tnnt3-primary-antibodies-ihc-testing-2.jpgAnti-TNNT3 Antibody Picoband®IHC analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). <br>TNNT3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNT3 Antibody (A05885-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05885-2-tnnt3-primary-antibodies-ihc-testing-3.jpgAnti-TNNT3 Antibody Picoband®IHC analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). <br>TNNT3 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNT3 Antibody (A05885-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-trpv3-picoband-trade-antibody-a03874-1-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03874-1-TRPV3-primary-antibodies-IHC-testing-1.jpgAnti-TRPV3 Antibody Picoband® IHC analysis of TRPV3 using anti-TRPV3 antibody (A03874-1).
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TRPV3 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV3 Antibody (A03874-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03874-1-TRPV3-primary-antibodies-IHC-testing-2.jpgAnti-TRPV3 Antibody Picoband® IHC analysis of TRPV3 using anti-TRPV3 antibody (A03874-1).
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TRPV3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV3 Antibody (A03874-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03874-1-TRPV3-primary-antibodies-FC-testing-3.jpgAnti-TRPV3 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-TRPV3 antibody (A03874-1). <br> Overlay histogram showing A431 cells stained with A03874-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV3 Antibody (A03874-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03874-1-TRPV3-primary-antibodies-FC-testing-4.jpgAnti-TRPV3 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-TRPV3 antibody (A03874-1). <br> Overlay histogram showing CACO-2 cells stained with A03874-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV3 Antibody (A03874-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03874-1-TRPV3-primary-antibodies-WB-testing-5.jpgAnti-TRPV3 Antibody Picoband® Western blot analysis of TRPV3 using anti-TRPV3 antibody (A03874-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human MDA-MB-453 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV3 antigen affinity purified polyclonal antibody (Catalog # A03874-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV3 at approximately 100KD. The expected band size for TRPV3 is at 91KD.
https://www.bosterbio.com/products/primary-antibodies/anti-trpv5-picoband-trade-antibody-a03218-1-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-IHC-testing-1.jpgAnti-TRPV5 Antibody Picoband® IHC analysis of TRPV5 using anti-TRPV5 antibody (A03218-1).
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TRPV5 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (A03218-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-IHC-testing-2.jpgAnti-TRPV5 Antibody Picoband® IHC analysis of TRPV5 using anti-TRPV5 antibody (A03218-1).
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TRPV5 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (A03218-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-IHC-testing-3.jpgAnti-TRPV5 Antibody Picoband® IHC analysis of TRPV5 using anti-TRPV5 antibody (A03218-1).
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TRPV5 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPV5 Antibody (A03218-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-FC-testing-4.jpgAnti-TRPV5 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TRPV5 antibody (A03218-1). <br> Overlay histogram showing HepG2 cells stained with A03218-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (A03218-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-FC-testing-5.jpgAnti-TRPV5 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TRPV5 antibody (A03218-1). <br> Overlay histogram showing U87 cells stained with A03218-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV5 Antibody (A03218-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03218-1-TRPV5-primary-antibodies-WB-testing-6.jpgAnti-TRPV5 Antibody Picoband® Western blot analysis of TRPV5 using anti-TRPV5 antibody (A03218-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: monkey COS-7 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # A03218-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83KD. The expected band size for TRPV5 is at 83KD.
https://www.bosterbio.com/products/primary-antibodies/anti-thioredoxin-2-txn2-antibody-a04586-1-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-1.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
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TXN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04586-1-txn2-primary-antibodies-ihc-testing-10.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1). <br> TXN2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-2.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
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TXN2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-3.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
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TXN2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-4.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
<br>
TXN2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-5.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
<br>
TXN2 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-6.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
<br>
TXN2 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-7.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
<br>
TXN2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-8.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
<br>
TXN2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A04586-1-TXN2-primary-antibodies-IHC-testing-9.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IHC analysis of TXN2 using anti-TXN2 antibody (A04586-1).
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TXN2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04586-1-txn2-primary-antibodies-if-testing-11.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® IF analysis of TXN2 using anti-TXN2 antibody (A04586-1). <br> TXN2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TXN2 Antibody (A04586-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04586-1-txn2-primary-antibodies-fc-testing-12.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TXN2 antibody (A04586-1). <br> Overlay histogram showing THP-1 cells stained with A04586-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXN2 Antibody (A04586-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04586-1-txn2-primary-antibodies-fc-testing-13.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TXN2 antibody (A04586-1). <br> Overlay histogram showing U87 cells stained with A04586-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXN2 Antibody (A04586-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04586-1-txn2-primary-antibodies-wb-testing-14.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® Western blot analysis of TXN2 using anti-TXN2 antibody (A04586-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat heart tissue lysates,<br>
Lane 6: rat kidney tissue lysates,<br>
Lane 7: mouse heart tissue lysates,<br>
Lane 8: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXN2 antigen affinity purified polyclonal antibody (Catalog # A04586-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXN2 at approximately 14 kDa. The expected band size for TXN2 is at 14 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-b7h4-vtcn1-picoband-trade-antibody-a02821-3-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02821-3-vtcn1-primary-antibodies-wb-testing-1.jpgAnti-B7H4/VTCN1 Antibody Picoband® Western blot analysis of VTCN1 using anti-VTCN1 antibody (A02821-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: mouse liver tissue lysates,<br>
Lane 7: moue liver tissue lysates,<br>
Lane 8: mouse HEPA1-6 whole cell lysates,<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VTCN1 antigen affinity purified polyclonal antibody (Catalog # A02821-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VTCN1 at approximately 75 kDa. The expected band size for VTCN1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02821-3-vtcn1-primary-antibodies-ihc-testing-2.jpgAnti-B7H4/VTCN1 Antibody Picoband® IHC analysis of VTCN1 using anti-VTCN1 antibody (A02821-3). <br>
VTCN1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VTCN1 Antibody (A02821-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02821-3-vtcn1-primary-antibodies-if-testing-3.jpgAnti-B7H4/VTCN1 Antibody Picoband® IF analysis of VTCN1 using anti-VTCN1 antibody (A02821-3). <br>
VTCN1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-VTCN1 Antibody (A02821-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02821-3-vtcn1-primary-antibodies-fcm-testing-4.jpgAnti-B7H4/VTCN1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-VTCN1 antibody (A02821-3).<br>Overlay histogram showing U20S cells stained with A02821-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VTCN1 Antibody (A02821-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tnf-alpha-picoband-trade-antibody-a00002-4-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00002-4-tnf-alpha-primary-antibodies-wb-testing-1.jpgAnti-TNF alpha Antibody Picoband® Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-4). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates, <br> Lane 2: mouse thymus tissue lysates, <br> Lane 3: mouse liver tissue lysates, <br> Lane 4: mouse lung tissue lysates, <br> Lane 5: mouse kidney tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-decorin-dcn-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0749-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0749.pngHuman Decorin / DCN ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Decorin EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-g-csf-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0360-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0360.pngHuman G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human G-CSF EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-gm-csf-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0364-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0364.pngHuman GM-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human GM-CSF EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-ifn-gamma-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0373-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0373.pngHuman IFN Gamma ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IFN gamma EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-10-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0416-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0416.pngHuman IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-10 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-12-p70-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0421-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0421.pngHuman IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-12(p70) EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-2-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0397-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0397.pngHuman IL-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-2 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-3-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0402-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0402.pngHuman IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-3 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/human-il-4-ez-set-elisa-kit-ez0404-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0404.pngHuman IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-4 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-5-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0407-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0407.pngHuman IL-5 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-5 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-6-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0410-boster.html2026-03-24T05:25:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0410.pngHuman IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-6 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-7-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0779-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0779.pngHuman IL-7 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-7 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-se-selectin-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0502-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0502.pngMouse sE-Selectin ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse sE-Selectin EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-leptin-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0437-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0437.pngHuman Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human Leptin EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/rat-il-4-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0406-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0406.pngRat IL-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Rat IL-4 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-il-8-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0413-boster.html2026-02-04T06:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0413.pngHuman IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human IL-8 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-6-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0411-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0411.pngMouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-6 EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-bdnf-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0307-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/E/Z/EZ0307.pngHuman BDNF EZ-Set™ ELISA Kit (DIY Antibody Pairs)Human BDNF EZ Set ELISA Kit standard curvehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0307.pngHuman BDNF EZ-Set™ ELISA Kit (DIY Antibody Pairs)Human BDNF EZ-Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-bdnf-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0309-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0309.pngMouse BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse BDNF EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/rat-bdnf-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0308-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0308.pngRat BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs)Rat BDNF EZ Set ELISA Kit standard curve
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-mouse-cytokine-stripwells-15-plex-mek1002-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Mouse Cytokine - Stripwells (15-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-mouse-cytokine-inflammation-13-plex-mek1003-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Mouse Cytokine - Inflammation (13-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-rat-cytokine-inflammation-9-plex-mek1004-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Rat Cytokine - Inflammation (9-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-cytokine-screen-16-plex-mek1005-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Cytokine - Screen (16-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-mouse-cytokine-screen-15-plex-mek1006-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Mouse Cytokine - Screen (15-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-cytokine-hs-screen-15-plex-mek1007-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Cytokine HS Screen (15-Plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-cytokine-inflammation-9-plex-mek1008-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Cytokine - Inflammation Panel 1 (9-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-cytokine-panel-1-6-plex-mek1010-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Cytokine Panel 1 (6-Plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-mouse-cytokine-panel-1-6-plex-mek1011-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Mouse Cytokine Panel 1 (6-Plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-mouse-cytokine-panel-2-4-plex-mek1016-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Mouse Cytokine Panel 2 (4-Plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-porcine-cytokine-array-4-plex-mek1018-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Porcine Cytokine Array (4-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-chemokine-9-plex-mek1019-boster.html2026-03-24T05:25:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Chemokine (9-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-chemokine-4-plex-mek1020-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Chemokine (4-Plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-angiogenesis-9-plex-mek1021-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Angiogenesis (9-plex)
https://www.bosterbio.com/products/multiplex-elisa-kit-for-human-female-hormone-8-plex-mek1022-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Female Hormone (6-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-mmp-6-plex-mek1023-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human MMP (6-plex)
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-micronutrient-array-7-plex-mek1024-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMultiplex ELISA Kit For Human Micronutrient Array (7-plex)
https://www.bosterbio.com/products/all-secondary-antibodies/dylight-reg-594-conjugated-goat-anti-mouse-igg-ba1141-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1141_1.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro594 ConjugatedDDX5 was detected in paraffin-embedded sections of mouse intestine tissues using mouse anti-DDX5 Antigen Affinity purified monoclonal antibody (Catalog # M00670-1). Fluoro594 Conjugated Goat Anti-Mouse IgG (BA1141) was used to detect the primary antibody.
https://www.bosterbio.com/products/all-secondary-antibodies/dylight-reg-594-conjugated-goat-anti-rabbit-igg-ba1142-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1142_1.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 ConjugatedABCB5 was detected in immunocytochemical section of A431 cells using rabbit anti-ABCB5 Antigen Affinity purified polyclonal antibody (Catalog # A02979-1). Fluoro594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used to detect the primary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1142-1_1.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 ConjugatedFibrillarin/FBL was detected in immunocytochemical section of A549 cells using rabbit anti-Fibrillarin/FBL Antigen Affinity purified polyclonal antibody (Catalog # A03178-1). Fluoro594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used to detect the primary antibody.
https://www.bosterbio.com/products/primary-antibodies/anti-aldolase-aldoa-picoband-trade-antibody-a05022-3-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-3-aldoa-primary-antibodies-wb-testing-1.jpgAnti-Aldolase/ALDOA Antibody Picoband® Western blot analysis of Aldolase/ALDOA using anti-Aldolase/ALDOA antibody (A05022-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: rat thymus tissue lysates, <br>
Lane 4: mouse thymus tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase/ALDOA antigen affinity purified polyclonal antibody (Catalog # A05022-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aldolase/ALDOA at approximately 39 kDa. The expected band size for Aldolase/ALDOA is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-3-aldoa-primary-antibodies-ihc-testing-2.jpgAnti-Aldolase/ALDOA Antibody Picoband® IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). <br>ALDOA was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05022-3-ALDOA-primary-antibodies-IHC-testing-3.jpgAnti-Aldolase/ALDOA Antibody Picoband® IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). <br>ALDOA was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05022-3-ALDOA-primary-antibodies-IHC-testing-4.jpgAnti-Aldolase/ALDOA Antibody Picoband® IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). <br>ALDOA was detected in paraffin-embedded section of mouse heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05022-3-ALDOA-primary-antibodies-IHC-testing-5.jpgAnti-Aldolase/ALDOA Antibody Picoband® IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). <br>ALDOA was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05022-3-ALDOA-primary-antibodies-IHC-testing-6.jpgAnti-Aldolase/ALDOA Antibody Picoband® IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). <br>ALDOA was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05022-3-aldoa-primary-antibodies-if-testing-7.jpgAnti-Aldolase/ALDOA Antibody Picoband® IF analysis of Aldolase/ALDOA using anti- Aldolase/ALDOA antibody (A05022-3). <br>
Aldolase/ALDOA was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- Aldolase/ALDOA Antibody (A05022-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A05022-3-ALDOA-primary-antibodies-FC-testing-8.pngAnti-Aldolase/ALDOA Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-ALDOA antibody (A05022-3). <br>Overlay histogram showing U20S cells stained with A05022-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDOA Antibody (A05022-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-aldolase-aldob-picoband-trade-antibody-a03893-2-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-wb-testing-1.jpgAnti-Aldolase/ALDOB Antibody Picoband® Western blot analysis of ALDOB using anti-ALDOB antibody (A03893-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: rat liver tissue lysates, <br>
Lane 7: mouse liver tissue lysates, <br>
Lane 8: monkey liver tissue lysates, <br>
Lane 9: monkey kidney tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOB antigen affinity purified polyclonal antibody (Catalog # A03893-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDOB at approximately 39KD. The expected band size for ALDOB is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-ihc-testing-2.jpgAnti-Aldolase/ALDOB Antibody Picoband® IHC analysis of ALDOB using anti-ALDOB antibody (A03893-2).<br>
ALDOB was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOB Antibody (A03893-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-ihc-testing-3.jpgAnti-Aldolase/ALDOB Antibody Picoband® IHC analysis of ALDOB using anti-ALDOB antibody (A03893-2).<br>
ALDOB was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDOB Antibody (A03893-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-fc-testing-4.jpgAnti-Aldolase/ALDOB Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ALDOB antibody (A03893-2). <br>Overlay histogram showing SiHa cells stained with A03893-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDOB Antibody (A03893-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-alob-primary-antibodies-if-testing-5.jpgAnti-Aldolase/ALDOB Antibody Picoband® IF analysis of Aldolase/ALDOB using anti-Aldolase/ALDOB antibody (A03893-2).<br>
Aldolase/ALDOB was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Aldolase/ALDOB Antibody (A03893-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-if-testing-6.jpgAnti-Aldolase/ALDOB Antibody Picoband® IF analysis of ALDOB using anti-ALDOB antibody (A03893-2). <br>
ALDOB was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDOB Antibody (A03893-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03893-2-aldob-primary-antibodies-if-testing-7.jpgAnti-Aldolase/ALDOB Antibody Picoband® IF analysis of ALDOB using anti-ALDOB antibody (A03893-2). <br>
ALDOB was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDOB Antibody (A03893-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-apolipoprotein-l1-apol1-picoband-trade-antibody-a01841-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01841-1-APOL1-primary-antibodies-WB-testing-1.jpgAnti-Apolipoprotein L1/APOL1 Antibody Picoband® Western blot analysis of APOL1 using anti-APOL1 antibody (A01841-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human plasma tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOL1 antigen affinity purified polyclonal antibody (Catalog # A01841-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOL1 at approximately 37KD. The expected band size for APOL1 is at 44KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01841-1-apol1-primary-antibodies-ihc-testing-2.jpgAnti-Apolipoprotein L1/APOL1 Antibody Picoband® IHC analysis of APOL1 using anti-APOL1 antibody (A01841-1). <br> APOL1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APOL1 Antibody (A01841-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01841-1-apol1-primary-antibodies-ihc-testing-3.jpgAnti-Apolipoprotein L1/APOL1 Antibody Picoband® IHC analysis of APOL1 using anti-APOL1 antibody (A01841-1). <br> APOL1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APOL1 Antibody (A01841-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-bmal1-arntl-picoband-trade-antibody-a00260-3-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00260-3-arntl-primary-antibodies-wb-testing-1.jpgAnti-BMAL1/ARNTL Antibody Picoband® Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates, <br>
Lane 2: human HT1080 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat C6 whole cell lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse heart tissue lysates, <br>
Lane 9: mouse Neuro-2a whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-asic1-picoband-trade-antibody-a02332-2-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02332-2-ASIC1-primary-antibodies-WB-testing-1.jpgAnti-ASIC1 Antibody Picoband® Western blot analysis of ASIC1 using anti-ASIC1 antibody (A02332-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC1 antigen affinity purified polyclonal antibody (Catalog # A02332-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC1 at approximately 60KD. The expected band size for ASIC1 is at 60KD.
https://www.bosterbio.com/products/primary-antibodies/anti-atrx-picoband-trade-antibody-a00203-2-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00203-2-atrx-primary-antibodies-wb-testing-1.jpgAnti-ATRX Antibody Picoband®Western blot analysis of ATRX using anti-ATRX antibody (A00203-2). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human MOLT4 whole cell lysates,<br>
Lane 4: rat C6 whole cell lysates,<br>
Lane 5: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (A00203-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATRX at approximately 300 kDa. The expected band size for ATRX is at 283 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00203-2-atrx-primary-antibodies-ihc-testing-1.jpgAnti-ATRX Antibody Picoband®IHC analysis of ATRX using anti-ATRX antibody (A00203-2). <br>ATRX was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00203-2-atrx-primary-antibodies-ihc-testing-2.jpgAnti-ATRX Antibody Picoband®IHC analysis of ATRX using anti-ATRX antibody (A00203-2). <br>ATRX was detected in a paraffin-embedded section of human prostate tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATRX Antibody (A00203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-avpr1a-v1ar-picoband-trade-antibody-a03279-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03279-AVPR1A-primary-antibodies-WB-testing-1.jpgAnti-AVPR1A/V1aR Antibody Picoband® Western blot analysis of AVPR1A using anti-AVPR1A antibody (A03279). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human A431 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AVPR1A antigen affinity purified polyclonal antibody (Catalog # A03279) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AVPR1A at approximately 60KD. The expected band size for AVPR1A is at 47KD.
https://www.bosterbio.com/products/primary-antibodies/anti-bubr1-bub1b-picoband-trade-antibody-a01564-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-wb-testing-1.jpgAnti-BubR1/BUB1B Antibody Picoband® Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human Hek293 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human HL-60 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BubR1/BUB1B antigen affinity purified polyclonal antibody (Catalog # A01564-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 120-130KD. The expected band size for BubR1/BUB1B is at 120-130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-ihc-testing-10.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
BubR1/BUB1B was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BubR1/BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g001.jpgAnti-BubR1/BUB1B Antibody Picoband®Pan-cancer analysis of BUB1B expression. ( A ) Differential expression of BUB1B between tumor and normal tissues in pan-cancer analysis. ( B , C ) Expression of BUB1B in various cancer cell lines and tissues. ( D,E ) Cellular localization of BUB1B from U-251MG and U2OS. ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g002.jpgAnti-BubR1/BUB1B Antibody Picoband®BUB1B expression correlates with overall survival time (OS). GEPIA2 analyses of the association between BUB1B expression and OS in ( A ) ACC, ( B ) KIRC, ( C ) KIRP, ( D ) LGG, ( E ) LIHC, ( F ) LUAD, ( G ) MESO, ( H ) PAAD, ( I ) SARC, and ( J ) THYM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g003.jpgAnti-BubR1/BUB1B Antibody Picoband®Correlation between BUB1B expression and mutations in various cancer types. ( A ) Landscape of BUB1B mutation in 32 cancer types, ( B ) the subtypes and distributions of BUB1B somatic mutations, ( C , D ) Spearman correlation analysis for TMB, MSI, and BUB1B gene expression. In the figure, the horizontal axis represents the correlation coefficient between the genes and TMB, and the vertical axis represents the different tumors. The size of the dots in the figure represents the correlation coefficient, and the different colors represent the significance of the p value. The bluer the color in the diagram, the smaller the p value.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g004.jpgAnti-BubR1/BUB1B Antibody Picoband®The association between BUB1B expression and immune cell infiltration. ( A , B ) BUB1B expression is positively associated with MDSC infiltration in pan-cancer. ( C , D ) BUB1B expression is positively negative with NKT cell infiltration in pan-cancer. ( E – H ) BUB1B expression is negative associated with the infiltration level of B cells, macrophage cells, CD4+ T cells, and CD8+ T cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g005.jpgAnti-BubR1/BUB1B Antibody Picoband®The enrichment analysis of BUB1B co-expression genes in LUAD. ( A ) The BUB1B co-expression genes in LUAD. ( B , C ) The top 50 genes positively and negatively correlated to BUB1B. ( D , E ) GO and KEGG analysis of BUB1B co-expression genes in the LUAD cohort.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g006.jpgAnti-BubR1/BUB1B Antibody Picoband®Function of BUB1B in LUAD determined using the CancerSEA database. ( A ) Analysis from the CancerSEA database at single-cell resolution indicated that BUB1B was primarily involved in cell cycle, proliferation, DNA damage, DNA repair, invasion, inflammation, quiescence. ( B , C ) Functional relevance in LUAD, BUB1B expression was significantly positively correlated with cell cycle, proliferation, DNA damage, DNA repair, invasion, and was negatively correlated with inflammation, quiescence. The experiments were repeated three times. (* p < 0.05, ** p < 0.01, *** p < 0.001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-ijms-26-02061-g007.jpgAnti-BubR1/BUB1B Antibody Picoband®Cellular functions of BUB1B. ( A , B ) Western blot and RT-qPCR detection after siRNA-mediated knockdown of BUB1B in A549 cells. ( C ) CCK-8 assay results showing the decrease in the viability of A549 cells upon the knockdown of BUB1B. ( D , E ) Wound healing test. ( F , G ) Transwell assay results showing the decrease in the migration in A549 cells upon the knockdown of BUB1B. ( H , I ) Western blot results showing the decrease in the EMT progression in A549 cells upon the knockdown of BUB1B. All experiments were repeated three times with three replicates for each repeat. * p < 0.05, ** p < 0.01, and *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11899737/'>40076684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01564-1-BUB1B-primary-antibodies-IHC-testing-2.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BUB1B using anti-BUB1B antibody (A01564-1). <br>BUB1B was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01564-1-BUB1B-primary-antibodies-IHC-testing-3.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BUB1B using anti-BUB1B antibody (A01564-1). <br>BUB1B was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01564-1-BUB1B-primary-antibodies-IHC-testing-4.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BUB1B using anti-BUB1B antibody (A01564-1). <br>BUB1B was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01564-1-BUB1B-primary-antibodies-IHC-testing-5.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BUB1B using anti-BUB1B antibody (A01564-1). <br>BUB1B was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01564-1-BUB1B-primary-antibodies-FC-testing-6.pngAnti-BubR1/BUB1B Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Calpain 2 antibody (A03492). <br>Overlay histogram showing U87 cells stained with A03492 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpain 2 Antibody (A03492, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-if-testing-7.jpgAnti-BubR1/BUB1B Antibody Picoband® IF analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
BubR1/BUB1B was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-BubR1/BUB1B Antibody (A01564-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-ihc-testing-8.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
BubR1/BUB1B was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BubR1/BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-ihc-testing-9.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BubR1/BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01564-1-bub1b-primary-antibodies-ihc-testing-11.jpgAnti-BubR1/BUB1B Antibody Picoband® IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (A01564-1). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BubR1/BUB1B Antibody (A01564-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-calreticulin-calr-picoband-trade-antibody-a00894-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-WB-testing-1.jpgAnti-Calreticulin/CALR Antibody Picoband® Western blot analysis of CALR using anti-CALR antibody (A00894-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates, <br>Lane 2: human U-87MG whole cell lysates, <br>Lane 3: human U-937 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates, <br>Lane 5: human THP-1 whole cell lysates, <br>Lane 6: human T-47D whole cell lysates, <br>Lane 7: human PC-3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-IHC-testing-2.jpgAnti-Calreticulin/CALR Antibody Picoband® IHC analysis of CALR using anti-CALR antibody (A00894-1). <br>CALR was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-IHC-testing-3.jpgAnti-Calreticulin/CALR Antibody Picoband® IHC analysis of CALR using anti-CALR antibody (A00894-1). <br>CALR was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-IHC-testing-4.jpgAnti-Calreticulin/CALR Antibody Picoband® IHC analysis of CALR using anti-CALR antibody (A00894-1). <br>CALR was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-IHC-testing-5.jpgAnti-Calreticulin/CALR Antibody Picoband® IHC analysis of CALR using anti-CALR antibody (A00894-1). <br>CALR was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-IHC-testing-6.jpgAnti-Calreticulin/CALR Antibody Picoband® IHC analysis of CALR using anti-CALR antibody (A00894-1). <br>CALR was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CALR Antibody (A00894-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00894-1-CALR-primary-antibodies-FC-testing-7.pngAnti-Calreticulin/CALR Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CALR antibody (A00894-1). <br>Overlay histogram showing HepG2 cells stained with A00894-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CALR Antibody (A00894-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cebp-alpha-cebpa-picoband-trade-antibody-a00386-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00386-1-fcell-12-1503481-g004.jpgAnti-CEBP Alpha/CEBPA Antibody Picoband®ANXA1 mediated PPARγ-CEBPα pathway to regulate osteoclast differentiation (A) The mRNA level of PPARγ in RAW264.7 cultured with siANXA1-EVs. (B) The mRNA level of CEBPα in RAW264.7 cultured with siANXA1-EVs. (C) The protein level of PPARγ and CEBPα in RAW264.7 cultured with siANXA1-EVs. (D) Quantitative analysis of PPARγ protein expression. (E) Quantitative analysis of CEBPα protein expression. (F) Schematic illustration of PPARγ inhibited RAW264.7 and DFSC-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) PPARγ inhibited RAW264.7 construction. (J) The mRNA level of CEBPα in PPARγ inhibited RAW264.7. (K) The protein level of PPARγ and CEBPα in PPARγ inhibited RAW264.7. (L) Quantitative analysis of PPARγ protein expression. (M) Quantitative analysis of CEBPα protein expression. (N) The mRNA level of ACP5 , CTSK and CFOS in PPARγ inhibited RAW264.7. (O) The protein level of ACP5, CTSK and CFOS in PPARγ inhibited RAW264.7. (P) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00386-1-fcell-12-1503481-g005.jpgAnti-CEBP Alpha/CEBPA Antibody Picoband®DFSCs-EVs/ANXA1 regulating tooth eruption by affecting osteoclast differentiation. (A) Representative micro-CT images of detecting tooth eruption distance. (B) Analysis of tooth eruption distance based on micro-CT. (C) Representative H&E staining images of the first mandibular molar area. (D) Analysis of tooth eruption distance based on H&E staining. (E) Representative images of TRAP staining. (F) Quantitative analysis of TRAP-positive area. (G) Representative immunohistochemistry staining images of PPARγ expression in the first mandibular molar area. (H) Quantitative analysis of PPARγ expression in the first mandibular molar area. (I) Representative immunohistochemistry staining images of CEBPα expression in the first mandibular molar area. (J) Quantitative analysis of CEBPα expression in the first mandibular molar area. ns, not significant. Scale bar = 1 mm ** p < 0. 01. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00386-1-cebp-primary-antibody-fcm-testing-3.pngAnti-CEBP Alpha/CEBPA Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-CEBP Alpha/CEBPA antibody (A00386-1). <br>Overlay histogram showing CACO-2 cells stained with A00386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Alpha/CEBPA Antibody (A00386-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00386-1-cebp-primary-antibody-wb-testing-1.jpgAnti-CEBP Alpha/CEBPA Antibody Picoband® Western blot analysis of CEBP using anti-CEBP antibody (A00386-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human U-937 whole cell lysates,<br>
Lane 3: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP antigen affinity purified polyclonal antibody (Catalog # A00386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP at approximately 42 kDa. The expected band size for CEBP is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00386-1-cebp-primary-antibody-if-testing-2.jpgAnti-CEBP Alpha/CEBPA Antibody Picoband® IF analysis of CEBP Alpha/CEBPA using anti- CEBP Alpha/CEBPA antibody (A00386-1). <br>
CEBP Alpha/CEBPA was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CEBP Alpha/CEBPA Antibody (A00386-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cetp-picoband-trade-antibody-a00309-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00309-1-cetp-primary-antibodies-wb-testing-1.jpgAnti-CETP Antibody Picoband® Western blot analysis of CETP using anti-CETP antibody (A00309-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HCCT tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: rat liver tissue lysates, <br>
Lane 5: rat PC-12 whole cell lysates, <br>
Lane 6: mouse liver tissue lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CETP antigen affinity purified polyclonal antibody (Catalog # A00309-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CETP at approximately 75 kDa. The expected band size for CETP is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-2.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-3.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-4.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-5.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-6.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-IHC-testing-7.jpgAnti-CETP Antibody Picoband® IHC analysis of CETP using anti-CETP antibody (A00309-1). <br>CETP was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CETP Antibody (A00309-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00309-1-CETP-primary-antibodies-FC-testing-8.pngAnti-CETP Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CETP antibody (A00309-1). <br>Overlay histogram showing HepG2 cells stained with A00309-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CETP Antibody (A00309-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd35-cr1-picoband-trade-antibody-a01022-1-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01022-1-cd35-primary-antibodies-wb-testing-1.jpgAnti-CD35/CR1 Antibody Picoband® Western blot analysis of CD35 using anti-CD35 antibody (A01022-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human TF1 whole cell lysates, <br>
Lane 2: human TF1 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD35 antigen affinity purified polyclonal antibody (Catalog # A01022-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD35 at approximately 250 kDa. The expected band size for CD35 is at 224 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01022-1-cd35-primary-antibodies-ihc-testing-2.jpgAnti-CD35/CR1 Antibody Picoband® IHC analysis of CD35 using anti-CD35 antibody (A01022-1). <br>
CD35 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD35 Antibody (A01022-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01022-1-cd35-primary-antibodies-ihc-testing-3.jpgAnti-CD35/CR1 Antibody Picoband® IHC analysis of CD35 using anti-CD35 antibody (A01022-1). <br>
CD35 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD35 Antibody (A01022-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01022-1-cd35-primary-antibodies-ihc-testing-4.jpgAnti-CD35/CR1 Antibody Picoband® IHC analysis of CD35 using anti-CD35 antibody (A01022-1). <br>
CD35 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD35 Antibody (A01022-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-torc1-crtc1-picoband-trade-antibody-a01951-2-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01951-2-crtc1-primary-antibodies-wb-testing-1_1.jpgAnti-TORC1/CRTC1 Antibody Picoband®Western blot analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human MCF-7 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human U251 whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat C6 whole cell lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1/CRTC1 antigen affinity purified polyclonal antibody (Catalog # A01951-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TORC1/CRTC1 at approximately 78 kDa. The expected band size for TORC1/CRTC1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01951-2-crtc1-primary-antibodies-if-testing-1.jpgAnti-TORC1/CRTC1 Antibody Picoband®IF analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody (A01951-2) and anti-Tubulin Alpha antibody (M03989-3).<br>
TORC1/CRTC1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TORC1/CRTC1 Antibody (A01951-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01951-2-crtc1-primary-antibodies-fcm-testing-1.jpgAnti-TORC1/CRTC1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TORC1/CRTC1 antibody (A01951-2). <br>
Overlay histogram showing MCF-7 cells stained with A01951-2 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TORC1/CRTC1 Antibody (A01951-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-2e1-cyp2e1-picoband-trade-antibody-a00672-boster.html2026-03-24T05:25:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-WB-testing-1.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates, <br>Lane 2: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # A00672) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00672-41065_2025_529_fig3_html.pngAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®( A ) Serum NAD + levels. ( B ) Serum NADH levels. C ) The NAD + /NADH ratio. ( D - H ) Western blots of CYP2E1, SIRT1, NAMPT, and NMNAT, with GAPDH as an internal control ( n = 5). ( E - H ) Image J was used to quantify the proteins CYP2E1, SIRT1, NAMPT, and NMNAT <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s41065-025-00529-x'>40819082</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-IHC-testing-2.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® IHC analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). <br>CYP2E1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (A00672) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-IHC-testing-3.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® IHC analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). <br>CYP2E1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (A00672) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-IHC-testing-4.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® IHC analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). <br>CYP2E1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (A00672) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-IHC-testing-5.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® IHC analysis of CYP2E1 using anti-CYP2E1 antibody (A00672). <br>CYP2E1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (A00672) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00672-CYP2E1-primary-antibodies-FC-testing-6.pngAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CYP2E1 antibody (A00672). <br>Overlay histogram showing SiHa cells stained with A00672 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP2E1 Antibody (A00672,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-dopamine-beta-hydroxylase-dbh-picoband-trade-antibody-a01110-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01110-dbh-primary-antibodies-wb-testing-1.jpgAnti-Dopamine beta Hydroxylase/DBH Antibody Picoband® Western blot analysis of DBH using anti-DBH antibody (A01110). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SH-SY5Y whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human U87 whole cell lysates, <br>
Lane 4: human U20S whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat C6 whole cell lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse Neuro-2a whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBH antigen affinity purified polyclonal antibody (Catalog # A01110) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for DBH at approximately 75 kDa. The expected band size for DBH is at 70 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-mck10-nep-ddr1-picoband-trade-antibody-a00905-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-WB-testing-1.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates, <br>Lane 2: human A549 whole cell lysates, <br>Lane 3: human K562 whole cell lysates, <br>Lane 4: human HepG2 whole cell lysates, <br>Lane 5: human PC-3 whole cell lysates, <br>Lane 6: human THP-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-WB-testing-2.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat lung tissue lysates, <br>Lane 3: rat testicular tissue lysates, <br>Lane 4: mouse brain tissue lysates, <br>Lane 5: mouse lung tissue lysates, <br>Lane 6: mouse testicular tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-IHC-testing-3.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IHC analysis of DDR1 using anti-DDR1 antibody (A00905). <br>DDR1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-IHC-testing-4.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IHC analysis of DDR1 using anti-DDR1 antibody (A00905). <br>DDR1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-IHC-testing-5.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IHC analysis of DDR1 using anti-DDR1 antibody (A00905). <br>DDR1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-IHC-testing-6.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IHC analysis of DDR1 using anti-DDR1 antibody (A00905). <br>DDR1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-IHC-testing-7.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IHC analysis of DDR1 using anti-DDR1 antibody (A00905). <br>DDR1 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00905-DDR1-primary-antibodies-FC-testing-8.pngAnti-MCK10/NEP/DDR1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-DDR1 antibody (A00905). <br>Overlay histogram showing A431 cells stained with A00905 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDR1 Antibody (A00905,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00905-ddr1-primary-antibodies-if-testing-9.jpgAnti-MCK10/NEP/DDR1 Antibody Picoband® IF analysis of DDR1 using anti-DDR1 antibody (A00905). <br>
DDR1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDR1 Antibody (A00905) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ddx5-picoband-trade-antibody-a00670-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00670-ddx5-primary-antibodies-wb-testing-1.jpgAnti-DDX5 Antibody Picoband® Western blot analysis of DDX5 using anti-DDX5 antibody (A00670). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX5 antigen affinity purified polyclonal antibody (Catalog # A00670) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX5 at approximately 69 kDa. The expected band size for DDX5 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00670-ddx5-primary-antibodies-ihc-testing-2.jpgAnti-DDX5 Antibody Picoband® IHC analysis of DDX5 using anti-DDX5 antibody (A00670). <br>DDX5 was detected in paraffin-embedded section of mouse small intestine tissues . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00670-DDX5-primary-antibodies-IHC-testing-3.jpgAnti-DDX5 Antibody Picoband® IHC analysis of DDX5 using anti-DDX5 antibody (A00670). <br>DDX5 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00670-DDX5-primary-antibodies-IHC-testing-4.jpgAnti-DDX5 Antibody Picoband® IHC analysis of DDX5 using anti-DDX5 antibody (A00670). <br>DDX5 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00670-DDX5-primary-antibodies-IHC-testing-5.jpgAnti-DDX5 Antibody Picoband® IHC analysis of DDX5 using anti-DDX5 antibody (A00670). <br>DDX5 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00670-DDX5-primary-antibodies-IHC-testing-6.jpgAnti-DDX5 Antibody Picoband® IHC analysis of DDX5 using anti-DDX5 antibody (A00670). <br>DDX5 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00670-ddx5-primary-antibodies-if-testing-7.jpgAnti-DDX5 Antibody Picoband® IF analysis of DDX5 using anti-DDX5 antibody (A00670). <br>
DDX5 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDX5 Antibody (A00670) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00670-ddx5-primary-antibodies-fc-testing-8.jpgAnti-DDX5 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-DDX5 antibody (A00670). <br>Overlay histogram showing HL-60 cells stained with A00670 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX5 Antibody (A00670,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00670-ddx5-primary-antibodies-ip-testing-9.jpgAnti-DDX5 Antibody Picoband® Immunoprecipitating DDX5 in A431 whole cell lysate .<br>Western blot analysis of DDX5 using anti-DDX5 antibody (A00670).<br>Lane 1: A431 whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-DDX5 antibody in A431 whole cell lysate, <br>Lane 3: anti-DDX5 antibody (2μg) + A431 whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX5 antigen affinity purified polyclonal antibody (A00670) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for DDX5 at approximately 69 kDa. The expected band size for DDX5 is at 69 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-exonuclease-1-exo1-picoband-trade-antibody-a00536-2-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-2-exo1-primary-antibodies-wb-testing-1.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband® Western blot analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exonuclease 1/EXO1 antigen affinity purified polyclonal antibody (Catalog # A00536-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exonuclease 1/EXO1 at approximately 120 kDa. The expected band size for Exonuclease 1/EXO1 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-2-exo1-primary-antibodies-ihc-testing-2.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband® IHC analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-2). <br>
Exonuclease 1/EXO1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Exonuclease 1/EXO1 Antibody (A00536-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-2-exo1-primary-antibodies-ihc-testing-3.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband® IHC analysis of Exonuclease 1/EXO1 using anti-Exonuclease 1/EXO1 antibody (A00536-2). <br>
Exonuclease 1/EXO1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Exonuclease 1/EXO1 Antibody (A00536-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-2-exo1-primary-antibodies-fcm-testing-4.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Exonuclease 1/EXO1 antibody (A00536-2). <br>Overlay histogram showing THP-1 cells stained with A00536-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Exonuclease 1/EXO1 Antibody (A00536-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-filamin-a-flna-picoband-trade-antibody-a00502-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-WB-testing-1.jpgAnti-Filamin A/FLNA Antibody Picoband® Western blot analysis of FLNA using anti-FLNA antibody (A00502-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates, <br>Lane 2: human U2OS whole cell lysates, <br>Lane 3: human Hela whole cell lysates, <br>Lane 4: human PC-3 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLNA antigen affinity purified polyclonal antibody (Catalog # A00502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLNA at approximately 281KD. The expected band size for FLNA is at 281KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-WB-testing-2.jpgAnti-Filamin A/FLNA Antibody Picoband® Western blot analysis of FLNA using anti-FLNA antibody (A00502-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat ovarian tissue lysates, <br>Lane 2: mouse lung tissue lysates, <br>Lane 3: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLNA antigen affinity purified polyclonal antibody (Catalog # A00502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLNA at approximately 281KD. The expected band size for FLNA is at 281KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-3.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-4.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-5.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-6.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-7.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00502-1-FLNA-primary-antibodies-IHC-testing-8.jpgAnti-Filamin A/FLNA Antibody Picoband® IHC analysis of FLNA using anti-FLNA antibody (A00502-1). <br>FLNA was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLNA Antibody (A00502-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00502-1-flna-primary-antibodies-fcm-testing-9.jpgAnti-Filamin A/FLNA Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-FLNA antibody (A00502-1). <br>
Overlay histogram showing U87 cells stained with A00502-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLNA Antibody (A00502-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00502-1-flna-primary-antibodies-if-testing-10.jpgAnti-Filamin A/FLNA Antibody Picoband® IF analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody (A00502-1). <br>
Filamin A/FLNA was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Filamin A/FLNA Antibody (A00502-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-foxc1-picoband-trade-antibody-a00962-2-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00962-2-foxc1-primary-antibodies-wb-testing-1_1.jpgAnti-FOXC1 Antibody Picoband® Western blot analysis of FOXC1 using anti-FOXC1 antibody (A00962-2). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXC1 antigen affinity purified polyclonal antibody (A00962-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FOXC1 at approximately 75 kDa. The expected band size for FOXC1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00962-2-foxc1-primary-antibodies-if-testing-2.jpgAnti-FOXC1 Antibody Picoband® IF analysis of FOXC1 using anti-FOXC1 antibody (A00962-2) and anti-Tubulin Alpha antibody (M03989-3).<br>
FOXC1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXC1 Antibody (A00962-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-metabotropic-glutamate-receptor-2-grm2-antibody-a06123-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06123-1-gca-primary-antibodies-wb-testing-1.jpgAnti-Metabotropic glutamate receptor 2/GRM2 Antibody Picoband® Western blot analysis of GRM2 using anti-GRM2 antibody (A06123-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM2 antigen affinity purified polyclonal antibody (Catalog # A06123-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM2 at approximately 100kDa and >200 kDa. The expected band size for GRM2 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06123-1-grm2-primary-antibodies-ihc-testing-2.jpgAnti-Metabotropic glutamate receptor 2/GRM2 Antibody Picoband® IHC analysis of GRM2 using anti-GRM2 antibody (A06123-1). <br>
GRM2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRM2 Antibody (A06123-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06123-1-grm2-primary-antibodies-fc-testing-3.pngAnti-Metabotropic glutamate receptor 2/GRM2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-GRM2 antibody (A06123-1). <br>Overlay histogram showing HepG2 cells stained with A06123-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRM2 Antibody (A06123-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-metabotropic-glutamate-receptor-5-grm5-antibody-a01338-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01338-1-GRM5-primary-antibodies-WB-testing-1.jpgAnti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® Western blot analysis of GRM5 using anti-GRM5 antibody (A01338-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: mouse brain tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM5 at approximately 150KD. The expected band size for GRM5 is at 132KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01338-1-GRM5-primary-antibodies-FC-testing-2.pngAnti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-GRM5 antibody (A01338-1). <br>Overlay histogram showing U937 cells stained with A01338-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRM5 Antibody (A01338-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-beta-glucuronidase-gusb-picoband-trade-antibody-a02234-1-boster.html2026-04-03T05:00:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02234-1-gusb-primary-antibodies-wb-testing-1_1.jpgAnti-beta glucuronidase/GUSB Antibody Picoband® Western blot analysis of GUSB using anti-GUSB antibody (A02234-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUSB antigen affinity purified polyclonal antibody (Catalog # A02234-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GUSB at approximately 82KD. The expected band size for GUSB is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02234-1-gusb-primary-antibodies-ihc-testing-1.jpgAnti-beta glucuronidase/GUSB Antibody Picoband®IHC analysis of GUSB using anti-GUSB antibody (A02234-1). <br>
GUSB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GUSB Antibody (A02234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-1-GUSB-primary-antibodies-FC-testing-2.pngAnti-beta glucuronidase/GUSB Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-GUSB antibody (A02234-1). <br>Overlay histogram showing A431 cells stained with A02234-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GUSB Antibody (A02234-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02234-1-gusb-primary-antibodies-if-testing-3.jpgAnti-beta glucuronidase/GUSB Antibody Picoband® IF analysis of GUSB using anti-GUSB antibody (A02234-1). <br>
GUSB was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GUSB Antibody (A02234-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-beta-glucuronidase-gusb-picoband-trade-antibody-a02234-2-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-2-GUSB-primary-antibodies-WB-testing-1.jpgAnti-beta glucuronidase/Gusb Antibody Picoband® Western blot analysis of GUSB using anti-GUSB antibody (A02234-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates, <br>Lane 2: mouse liver tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUSB antigen affinity purified polyclonal antibody (Catalog # A02234-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GUSB at approximately 75KD. The expected band size for GUSB is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-2-GUSB-primary-antibodies-IHC-testing-2.jpgAnti-beta glucuronidase/Gusb Antibody Picoband® IHC analysis of GUSB using anti-GUSB antibody (A02234-2). <br>GUSB was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GUSB Antibody (A02234-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-2-GUSB-primary-antibodies-IHC-testing-3.jpgAnti-beta glucuronidase/Gusb Antibody Picoband® IHC analysis of GUSB using anti-GUSB antibody (A02234-2). <br>GUSB was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GUSB Antibody (A02234-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-2-GUSB-primary-antibodies-IHC-testing-4.jpgAnti-beta glucuronidase/Gusb Antibody Picoband® IHC analysis of GUSB using anti-GUSB antibody (A02234-2). <br>GUSB was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GUSB Antibody (A02234-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A02234-2-GUSB-primary-antibodies-IHC-testing-5.jpgAnti-beta glucuronidase/Gusb Antibody Picoband® IHC analysis of GUSB using anti-GUSB antibody (A02234-2). <br>GUSB was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GUSB Antibody (A02234-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-histone-h1-0-h1f0-picoband-trade-antibody-a08821-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08821-1-h1f0-primary-antibodies-wb-testing-1.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® Western blot analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (A08821-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human Caco-2 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H1.0/H1F0 antigen affinity purified polyclonal antibody (Catalog # A08821-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H1.0/H1F0 at approximately 24 kDa. The expected band size for Histone H1.0/H1F0 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08821-1-h1f0-primary-antibodies-ihc-testing-2.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br> H1F0 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-3.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of mouse small intestine tissues . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-4.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of mouse small intestine tissues . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-5.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-6.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-7.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-8.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-9.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). <br>H1F0 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-10.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). H1F0 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08821-1-H1F0-primary-antibodies-IHC-testing-11.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). H1F0 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08821-1-h1f0-primary-antibodies-fcm-testing-13_1.pngAnti-Histone H1.0/H1F0 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Histone H1.0/H1F0 antibody (A08821-1). <br>Overlay histogram showing U20S cells stained with A08821-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H1.0/H1F0 Antibody (A08821-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08821-1-h1f0-primary-antibodies-if-testing-12.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® IF analysis of Histone H1.0/H1F0 using anti- Histone H1.0/H1F0 antibody (A08821-1). <br>
Histone H1.0/H1F0 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- Histone H1.0/H1F0 Antibody (A08821-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-hif-1-alpha-hif1a-picoband-trade-antibody-a00013-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00013-1-HIF1A-primary-antibodies-WB-testing-1_1.jpgAnti-HIF-1 alpha/HIF1A Antibody Picoband® Western blot analysis of HIF1A using anti-HIF1A antibody (A00013-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123KD. The expected band size for HIF1A is at 93KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00013-1-41419_2020_2781_fig6_html.pngAnti-HIF-1 alpha/HIF1A Antibody Picoband®Upregulation of antioxidant and proproliferative genes during heart regeneration is impaired in the injury Δ113p53 M/M mutant hearts. a – f Relative mRNA expression of Δ113p53 ( a ), gpx1a ( b ), sesn2 ( c ), hif1al2 ( d ), jak2a ( e ) and pim2 ( f ) in the Δ113p53 +/+ and Δ113p53 M/M hearts at sham and 14 dpa. The total RNA was extracted from a pool of at least 10 hearts in each group. g Western blot was performed to analyse the induction of zebrafish Hif1α in different samples as indicated. The Δ113p53 +/+ and Δ113p53 M/M mutant zebrafish with heart resection were treated with DPI from 3 to 7 dpa. Total protein was isolated from four hearts/treatment at 7 dpa and subjected to western blot analysis. Gapdh was used as the protein loading control. The experiments were repeated independently for at least three times with similar results. Statistical analysis was performed on relevant data using Student’s two-tailed t test in GraphPad Prism 5. The p values were represented by n.s. and asterisks. n.s., p > 0.05. * p < 0.05. ** p < 0.01. *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02781-7'>32703938</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-hla-dr-hla-dra-picoband-trade-antibody-a01195-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-wb-testing-1.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: human Daudi whole cell lysates, <br>
Lane 3: human Ramos whole cell lysates, <br>
Lane 4: human HMy2.CIR whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified polyclonal antibody (Catalog # A01195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 37 kDa. The expected band size for HLA-DRA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-ihc-testing-2.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). <br>HLA-DRA was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-DRA Antibody (A01195) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-ihc-testing-3.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). <br>HLA-DRA was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-DRA Antibody (A01195) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-ihc-testing-4.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).<br>
HLA-DRA was detected in paraffin-embedded section of human duodenal adenocarcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-DRA Antibody (A01195) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-ihc-testing-5.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).<br>
HLA-DRA was detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-DRA Antibody (A01195) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-ihc-testing-6.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).<br>
HLA-DRA was detected in paraffin-embedded section of human seminoma testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-DRA Antibody (A01195) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-fc-testing-7.pngAnti-HLA-DR/HLA-DRA Antibody Picoband®7. Flow Cytometry analysis of human PBMC cells using anti-HLA-DRA antibody (A01195). <br>Overlay histogram showing human PBMC cells stained with A01195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLA-DRA Antibody (A01195,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01195-hla-dra-primary-antibodies-fc-testing-8_1.pngAnti-HLA-DR/HLA-DRA Antibody Picoband® Flow Cytometry analysis of SW620 cells using anti-HLA-DRA antibody (A01195). <br>Overlay histogram showing SW620 cells stained with A01195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLA-DRA Antibody (A01195,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-iqgap1-picoband-trade-antibody-a01603-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-fcm-testing-6.jpgAnti-IQGAP1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti- IQGAP1 antibody (A01603-1). <br>Overlay histogram showing A431 cells stained with A01603-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- IQGAP1 Antibody (A01603-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-wb-testing-1.jpgAnti-IQGAP1 Antibody Picoband® Western blot analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: human SW620 whole cell lysates,<br>
Lane 3: human U20S whole cell lysates,<br>
Lane 4: human HEK293 whole cell lysates,<br>
Lane 5: human K562 whole cell lysates,<br>
Lane 6: human HepG2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP1 antigen affinity purified polyclonal antibody (Catalog # A01603-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP1 at approximately 195 kDa. The expected band size for IQGAP1 is at 189 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-ihc-testing-2.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). <br>
IQGAP1 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IQGAP1 Antibody (A01603-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-ihc-testing-3.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). <br>
IQGAP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IQGAP1 Antibody (A01603-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-ihc-testing-4.jpgAnti-IQGAP1 Antibody Picoband® IHC analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). <br>
IQGAP1 was detected in a paraffin-embedded section of human gallbladder squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IQGAP1 Antibody (A01603-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01603-1-iqgap1-primary-antibodies-if-testing-5.jpgAnti-IQGAP1 Antibody Picoband® IF analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). <br>
IQGAP1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-IQGAP1 Antibody (A01603-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-irak-1-irak1-picoband-trade-antibody-a01021-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-1-irak1-primary-antibodies-wb-testing-1.jpgAnti-IRAK-1/IRAK1 Antibody Picoband® Western blot analysis of IRAK1 using anti-IRAK1 antibody (A01021-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: rat liver tissue lysates,<br>
Lane 3: rat kidney tissue lysates,<br>
Lane 4: mouse liver tissue lysates,<br>
Lane 5: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK1 antigen affinity purified polyclonal antibody (Catalog # A01021-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRAK1 at approximately 77 kDa. The expected band size for IRAK1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-1-irak1-primary-antibodies-ihc-testing-2.jpgAnti-IRAK-1/IRAK1 Antibody Picoband® IHC analysis of IRAK1 using anti-IRAK1 antibody (A01021-1). <br>IRAK1 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRAK1 Antibody (A01021-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01021-1-IRAK1-primary-antibodies-IHC-testing-3.jpgAnti-IRAK-1/IRAK1 Antibody Picoband® IHC analysis of IRAK1 using anti-IRAK1 antibody (A01021-1). <br>IRAK1 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRAK1 Antibody (A01021-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01021-1-IRAK1-primary-antibodies-FC-testing-5.pngAnti-IRAK-1/IRAK1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-IRAK1 antibody (A01021-1). <br>Overlay histogram showing A549 cells stained with A01021-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRAK1 Antibody (A01021-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-1-irak1-primary-antibodies-if-testing-4.jpgAnti-IRAK-1/IRAK1 Antibody Picoband® IF analysis of IRAK1 using anti- IRAK1 antibody (A01021-1). <br>
IRAK1 was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-IRAK1 Antibody (A01021-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-c-kit-kit-picoband-trade-antibody-a01335-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01335-1-kit-primary-antibodies-wb-testing-1.jpgAnti-c-Kit/Kit Antibody Picoband® Western blot analysis of Kit using anti-Kit antibody (A01335-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human A549 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kit antigen affinity purified polyclonal antibody (Catalog # A01335-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kit at approximately 145 kDa. The expected band size for Kit is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01335-1-kit-primary-antibodies-ihc-testing-2.jpgAnti-c-Kit/Kit Antibody Picoband® IHC analysis of Kit using anti-Kit antibody (A01335-1). <br> Kit was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Kit Antibody (A01335-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01335-1-kit-primary-antibodies-ihc-testing-3.jpgAnti-c-Kit/Kit Antibody Picoband® IHC analysis of Kit using anti-Kit antibody (A01335-1). <br> Kit was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Kit Antibody (A01335-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-lpp-antibody-a01240-2-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-wb-testing-1_1.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® Western blot analysis of LPP using anti-LPP antibody (A01240-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human Hek293 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: rat ovary tissue lysates, <br>
Lane 6: rat heart tissue lysates, <br>
Lane 7: mouse ovary tissue lysates, <br>
Lane 8: mouse heart tissue lysates, <br>
Lane 9: mouse ANA-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # A01240-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 75KD. The expected band size for LPP is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-ihc-testing-2.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IHC analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-ihc-testing-3.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IHC analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-ihc-testing-4.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IHC analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-ihc-testing-5.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IHC analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-ihc-testing-6.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IHC analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-if-testing-7_1.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband® IF analysis of LPP using anti-LPP antibody (A01240-2). <br>
LPP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-LPP Antibody (A01240-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01240-2-lpp-primary-antibodies-fcm-testing-8.pngAnti-Lipoma-preferred partner LPP Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-LPP antibody (A01240-2). <br>Overlay histogram showing A431 cells stained with A01240-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LPP Antibody (A01240-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lrtomt-antibody-a13092-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-WB-testing-1.jpgAnti-LRTOMT Antibody Picoband® Western blot analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates, <br>Lane 2: human MCF-7 whole cell lysates, <br>Lane 3: human Hela whole cell lysates, <br>Lane 4: human Caco-2 whole cell lysates, <br>Lane 5: human K562 whole cell lysates, <br>Lane 6: human U20S whole cell lysates, <br>Lane 7: human THP-1 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRTOMT antigen affinity purified polyclonal antibody (Catalog # A13092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRTOMT at approximately 28KD. The expected band size for LRTOMT is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-WB-testing-2.jpgAnti-LRTOMT Antibody Picoband® Western blot analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat ovarian tissue lysates, <br>Lane 3: rat heart tissue lysates, <br>Lane 4: rat lung tissue lysates, <br>Lane 5: mouse brain tissue lysates, <br>Lane 6: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRTOMT antigen affinity purified polyclonal antibody (Catalog # A13092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRTOMT at approximately 28KD. The expected band size for LRTOMT is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-IHC-testing-3.jpgAnti-LRTOMT Antibody Picoband® IHC analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>LRTOMT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRTOMT Antibody (A13092-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-IHC-testing-4.jpgAnti-LRTOMT Antibody Picoband® IHC analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>LRTOMT was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRTOMT Antibody (A13092-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-IHC-testing-5.jpgAnti-LRTOMT Antibody Picoband® IHC analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>LRTOMT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRTOMT Antibody (A13092-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-IHC-testing-6.jpgAnti-LRTOMT Antibody Picoband® IHC analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>LRTOMT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRTOMT Antibody (A13092-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/1/A13092-1-LRTOMT-primary-antibodies-IHC-testing-7.jpgAnti-LRTOMT Antibody Picoband® IHC analysis of LRTOMT using anti-LRTOMT antibody (A13092-1). <br>LRTOMT was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRTOMT Antibody (A13092-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-mcm2-picoband-trade-antibody-a00374-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00374-1-mcm2-primary-antibodies-wb-testing-1.jpgAnti-MCM2 Antibody Picoband®Western blot analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human THP-1 whole cell lysates,<br>
Lane 4: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (A00374-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125 kDa. The expected band size for MCM2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00374-1-mcm2-primary-antibodies-wb-testing-2.jpgAnti-MCM2 Antibody Picoband®Western blot analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse NIH/3T3 whole cell lysates,<br>
Lane 2: mouse RAW264.7 whole cell lysates,<br>
Lane 3: mouse HEPA1-6 muscle tissue lysates,<br>
Lane 4: rat spleen tissue lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: mouse SP2/0 lysates,<br>
Lane 7: rat PC-12 whole cell lysates,<br>
Lane 8: rat RH35 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (A00374-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125 kDa. The expected band size for MCM2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-2.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-3.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of rat Lymphatic nodules tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-4.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of rat intestines tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-5.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-6.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-IHC-testing-7.jpgAnti-MCM2 Antibody Picoband® IHC analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br>MCM2 was detected in paraffin-embedded section of mouse Lymphatic nodules tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00374-1-mcm2-primary-antibodies-icc_if-testing-8.jpgAnti-MCM2 Antibody Picoband® IF analysis of MCM2 using anti-MCM2 antibody (A00374-1). <br> MCM2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM2 Antibody (A00374-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00374-1-MCM2-primary-antibodies-FC-testing-9.pngAnti-MCM2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-MCM2 antibody (A00374-1). <br>Overlay histogram showing HL-60 cells stained with A00374-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM2 Antibody (A00374-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-moesin-msn-picoband-trade-antibody-a00766-1-boster.html2026-03-24T05:25:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00766-1-MSN-primary-antibodies-WB-testing-1.jpgAnti-Moesin/MSN Antibody Picoband® Western blot analysis of MSN using anti-MSN antibody (A00766-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human MDA-MB-453 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00766-1-MSN-primary-antibodies-WB-testing-2.jpgAnti-Moesin/MSN Antibody Picoband® Western blot analysis of MSN using anti-MSN antibody (A00766-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat heart tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: rat spleen tissue lysates, <br>
Lane 5: mouse heart tissue lysates, <br>
Lane 6: mouse kidney tissue lysates, <br>
Lane 7: mouse liver tissue lysates, <br>
Lane 8: mouse spleen tissue lysates, <br>
Lane 9: mouse NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00766-1-msn-primary-antibodies-ihc-testing-3.jpgAnti-Moesin/MSN Antibody Picoband® IHC analysis of MSN using anti-MSN antibody (A00766-1). <br>
MSN was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MSN Antibody (A00766-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00766-1-msn-primary-antibodies-ihc-testing-4.jpgAnti-Moesin/MSN Antibody Picoband® IHC analysis of MSN using anti-MSN antibody (A00766-1). <br>
MSN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed inEDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MSN Antibody (A00766-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00766-1-msn-primary-antibodies-if-testing-5.jpgAnti-Moesin/MSN Antibody Picoband® IF analysis of MSN using anti-MSN antibody (A00766-1). <br>
MSN was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-MSN Antibody (A00766-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00766-1-msn-primary-antibodies-fcm-testing-6.pngAnti-Moesin/MSN Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-MSN antibody (A00766-1). <br>Overlay histogram showing U937 cells stained with A00766-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSN Antibody (A00766-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mtr-picoband-trade-antibody-a01140-2-boster.html2026-04-03T05:00:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-WB-testing-1.jpgAnti-MTR Antibody Picoband® Western blot analysis of MTR using anti-MTR antibody (A01140-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates, <br>Lane 2: human K562 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTR antigen affinity purified polyclonal antibody (Catalog # A01140-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTR at approximately 140KD. The expected band size for MTR is at 118KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-IHC-testing-2.jpgAnti-MTR Antibody Picoband® IHC analysis of MTR using anti-MTR antibody (A01140-2). <br>MTR was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTR Antibody (A01140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-IHC-testing-3.jpgAnti-MTR Antibody Picoband® IHC analysis of MTR using anti-MTR antibody (A01140-2). <br>MTR was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTR Antibody (A01140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-IHC-testing-4.jpgAnti-MTR Antibody Picoband® IHC analysis of MTR using anti-MTR antibody (A01140-2). <br>MTR was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTR Antibody (A01140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-IHC-testing-5.jpgAnti-MTR Antibody Picoband® IHC analysis of MTR using anti-MTR antibody (A01140-2). <br>MTR was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTR Antibody (A01140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-IHC-testing-6.jpgAnti-MTR Antibody Picoband® IHC analysis of MTR using anti-MTR antibody (A01140-2). <br>MTR was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTR Antibody (A01140-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01140-2-MTR-primary-antibodies-FC-testing-7.pngAnti-MTR Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-MTR antibody (A01140-2). <br>Overlay histogram showing SiHa cells stained with A01140-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTR Antibody (A01140-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ncam1-picoband-trade-antibody-a00184-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00184-ncam1-primary-antibodies-wb-testing-1.jpgAnti-NCAM1 Antibody Picoband®Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (A00184) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 120-200 kDa. The expected band size for NCAM1 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00184-NCAM1-primary-antibodies-FC-testing-2.pngAnti-NCAM1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-NCAM1 antibody (A00184). <br>Overlay histogram showing U937 cells stained with A00184 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCAM1 Antibody (A00184,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pxr-nr1i2-picoband-trade-antibody-a01133-3-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-WB-testing-1.jpgAnti-PXR/NR1I2 Antibody Picoband® Western blot analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HepG2 whole cell lysates, <br>Lane 2: human THP-1 whole cell lysates, <br>Lane 3: human HL-60 whole cell lysates, <br>Lane 4: human K562 whole cell lysates, <br>Lane 5: human A431 whole cell lysates, <br>Lane 6: human A549 whole cell lysates, <br>Lane 7: human Hela whole cell lysates, <br>Lane 8: human Caco-2 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1I2 antigen affinity purified polyclonal antibody (Catalog # A01133-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1I2 at approximately 50KD. The expected band size for NR1I2 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-WB-testing-2.jpgAnti-PXR/NR1I2 Antibody Picoband® Western blot analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat liver tissue lysates, <br>Lane 2: mouse liver tissue lysates, <br>Lane 3: mouse thymus tissue lysates, <br>Lane 4: mouse testicular tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1I2 antigen affinity purified polyclonal antibody (Catalog # A01133-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1I2 at approximately 50KD. The expected band size for NR1I2 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-IHC-testing-3.jpgAnti-PXR/NR1I2 Antibody Picoband® IHC analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). <br>NR1I2 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NR1I2 Antibody (A01133-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-IHC-testing-4.jpgAnti-PXR/NR1I2 Antibody Picoband® IHC analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). <br>NR1I2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NR1I2 Antibody (A01133-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-IHC-testing-5.jpgAnti-PXR/NR1I2 Antibody Picoband® IHC analysis of NR1I2 using anti-NR1I2 antibody (A01133-3). <br>NR1I2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NR1I2 Antibody (A01133-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01133-3-NR1I2-primary-antibodies-FC-testing-6.pngAnti-PXR/NR1I2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-NR1I2 antibody (A01133-3). <br>Overlay histogram showing HepG2 cells stained with A01133-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1I2 Antibody (A01133-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pcna-picoband-trade-antibody-a00125-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-13046_2019_1041_fig5_html.pngAnti-PCNA Antibody Picoband®CircSETD3 inhibits the growth of HCC through thecircSETD3/miR-421/MAPK14 pathway. a and b Both qRT-PCR and IHC showed MAPK14 was significantly downregulated in HCC tissues compared with matched non-tumorous tissues. c and d MAPK14 negatively correlated with miR-421 whereas positively correlated with circSETD3 in HCC tissues. e Schematic of MAPK14 wild-type (WT) and mutant (Mut) luciferase reporter vectors. f The relative luciferase activities were analyzed in 293 T cells co-transfected with miR-421 mimics or miR-mimics-NC and WT or Mut luciferase reporter vectors. g MiR-421 inhibitor up-regulated MAPK14 and down-regulated cyclinD1 and PCNA in Hep3B cells. MiR-421 mimics down-regulated MAPK14 and up-regulated cyclinD1 and PCNA in Huh7 cells. h CircSETD3 letivirus up-regulated MAPK14 and down-regulated cyclinD1 and PCNA in Huh7 cells, this effect could be reversed by co-transfected with miR-421 mimics. i circSETD3 siRNA down-regulated MAPK14 and up-regulated cyclinD1 and PCNA in Hep3B cells, this effect can be reversed by co-transfected with miR-421 inhibitors. HCC, hepatocellular carcinoma; qRT-PCR, quantitative reverse transcription polymerase chain reaction; in, inhibitors; mi, mimics; IHC, immunohistochemistry. ***P < 0.001. Error bars indicate SD <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1041-2'>30795787</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-13046_2019_1041_fig6_html.pngAnti-PCNA Antibody Picoband®CircSETD3 stably maintained in xenograft tumor models and inhibit tumor growth by targeting MAPK14. a and b Smaller tumor size and lower tumor weight were observed in circSETD3-overexpressing group. c Over-expressed circSETD3 could stably maintained in xenograft tumor models. d The expression of MAPK14 was increased, Ki-67, PCNA and Cyclin D1 were decreased in circSETD3-overexpressing group when compared with control group. **P < 0.01, ***P < 0.001. Error bars indicate SD <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1041-2'>30795787</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-13046_2018_922_fig7_html.pngAnti-PCNA Antibody Picoband®Effects of each treatment on the growth of human OS xenograft tumors. Nude mice bearing 143 human OS xenograft tumors were treated with CAP (20 mg/kg) and DDP (4 mg/kg) alone or in combination. The volumes of the xenograft tumors were measured at the indicated time points ( a ). The weight of each nude was measured at the indicated time points ( b ). After the last treatment, the mice were sacrificed, and representative images of the subcutaneous tumor xenografts in the nude mice and the morphology of the tumors are presented ( c ). Tumors were collected, and the tumor weights were measured and compared ( d ). Xenograft tumors were sectioned and stained with PCNA and Ki67 via IHC ( e ). Statistical analyses of the expression of PCNA and Ki67 in different groups ( f ). Tumors were sectioned and stained with H&E, and representative histopathological images are presented ( g ). Effects of the different treatments on renal histology. Representative histological profiles of kidneys after the different treatments were detected by H&E staining ( h ). Effects of the different treatments on BUN and creatinine levels in mice ( i ). The quantitative data are shown as the mean ± SD of 5 independent experiments; * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. the control (CAP-, DDP-) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-018-0922-0'>30326933</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-wb-testing-1.jpgAnti-PCNA Antibody Picoband® Western blot analysis of PCNA using anti-PCNA antibody (A00125). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human MCF-7 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-2.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-3.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-4.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-5.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human papillary thyroid carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-6.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-7.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-ihc-testing-8.jpgAnti-PCNA Antibody Picoband® IHC analysis of PCNA using anti-PCNA antibody (A00125). <br>
PCNA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCNA Antibody (A00125) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-if-testing-9.jpgAnti-PCNA Antibody Picoband® IF analysis of PCNA using anti-PCNA antibody (A00125) and anti-Beta Tubulin antibody (M01857-3).<br>
PCNA was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PCNA Antibody (A00125) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00125-pcna-primary-antibodies-fcm-testing-10.jpgAnti-PCNA Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PCNA antibody (A00125). <br>
Overlay histogram showing 293T cells stained with A00125 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCNA Antibody (A00125, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-pcsk9-picoband-trade-antibody-a00085-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00085-1-pcsk9-primary-antibodies-wb-testing-1.jpgAnti-PCSK9 Antibody Picoband® Western blot analysis of PCSK9 using anti-PCSK9 antibody (A00085-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human CACO-2 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: human Jurkat whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # A00085-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa, 80 kDa (precursor). The expected band size for PCSK9 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00085-1-pcsk9-primary-antibodies-ihc-testing-2.jpgAnti-PCSK9 Antibody Picoband® IHC analysis of PCSK9 using anti PCSK9 antibody (A00085-1). <br>
PCSK9 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PCSK9 Antibody (A00085-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00085-1-pcsk9-primary-antibodies-elisa-testing-3.jpgAnti-PCSK9 Antibody Picoband® Sandwich ELISA - Recombinant human PCSK9 protein standard curve.<br>
Use in combination with reagents from Human PCSK9 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1147).
https://www.bosterbio.com/products/primary-antibodies/anti-pitx2-rgs-picoband-trade-antibody-a01636-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01636-1-PITX2-primary-antibodies-WB-testing-1.jpgAnti-PITX2/RGS Antibody Picoband® Western blot analysis of PITX2 using anti-PITX2 antibody (A01636-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Caco-2 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
Lane 6: human U-87MG whole cell lysates, <br>
Lane 7: rat heart tissue lysates, <br>
Lane 8: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITX2 antigen affinity purified polyclonal antibody (Catalog # A01636-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITX2 at approximately 35KD. The expected band size for PITX2 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01636-1-pitx2-primary-antibodies-fcm-testing-2.pngAnti-PITX2/RGS Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PITX2 antibody (A01636-1).<br>Overlay histogram showing 293T cells stained with A01636-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITX2 Antibody (A01636-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-polycystin2-pkd2-picoband-trade-antibody-a00630-3-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00630-3-PKD2-primary-antibodies-WB-testing-1.jpgAnti-Polycystin 2/PKD2 Antibody Picoband® Western blot analysis of PKD2 using anti-PKD2 antibody (A00630-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human U-87MG whole cell lysates, <br>Lane 2: rat spleen tissue lysates, <br>Lane 3: mouse thymus tissue lysates, <br>Lane 4: mouse lung tissue lysates, <br>Lane 5: mouse SP20 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKD2 antigen affinity purified polyclonal antibody (Catalog # A00630-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKD2 at approximately 110KD. The expected band size for PKD2 is at 110KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00630-3-PKD2-primary-antibodies-IHC-testing-2.jpgAnti-Polycystin 2/PKD2 Antibody Picoband® IHC analysis of PKD2 using anti-PKD2 antibody (A00630-3). <br>PKD2 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKD2 Antibody (A00630-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00630-3-PKD2-primary-antibodies-FC-testing-3.pngAnti-Polycystin 2/PKD2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-PKD2 antibody (A00630-3). <br>Overlay histogram showing A549 cells stained with A00630-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKD2 Antibody (A00630-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-prmt1-picoband-trade-antibody-a01417-3-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01417-3-PRMT1-primary-antibodies-WB-testing-1.jpgAnti-PRMT1 Antibody Picoband® Western blot analysis of PRMT1 using anti-PRMT1 antibody (A01417-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HEK293 whole cell lysates, <br>Lane 2: human A549 whole cell lysates, <br>Lane 3: human Caco-2 whole cell lysates, <br>Lane 4: human PC-3 whole cell lysates, <br>Lane 5: human K562 whole cell lysates, <br>Lane 6: human Hela whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT1 antigen affinity purified polyclonal antibody (Catalog # A01417-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT1 at approximately 42-45KD. The expected band size for PRMT1 is at 42-45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01417-3-PRMT1-primary-antibodies-IHC-testing-2.jpgAnti-PRMT1 Antibody Picoband® IHC analysis of PRMT1 using anti-PRMT1 antibody (A01417-3). <br>PRMT1 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRMT1 Antibody (A01417-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01417-3-prmt1-primary-antibodies-fcm-testing-3.pngAnti-PRMT1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PRMT1 antibody (A01417-3). <br>
Overlay histogram showing 293T cells stained with A01417-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT1 Antibody (A01417-3, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-prmt5-antibody-a00635-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00635-1-PRMT5-primary-antibodies-WB-testing-1.jpgAnti-PRMT5 Antibody Picoband® Western blot analysis of PRMT5 using anti-PRMT5 antibody (A00635-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HL-60 whole cell lysates, <br>Lane 2: rat thymus tissue lysates, <br>Lane 3: mouse kidney tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified polyclonal antibody (Catalog # A00635-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 72KD. The expected band size for PRMT5 is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00635-1-PRMT5-primary-antibodies-FC-testing-2.pngAnti-PRMT5 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PRMT5 antibody (A00635-1). <br>Overlay histogram showing 293T cells stained with A00635-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT5 Antibody (A00635-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-p23-ptges3-picoband-trade-antibody-a04136-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04136-1-ptges3-primary-antibodies-wb-testing-1.jpgAnti-p23/PTGES3 Antibody Picoband® Western blot analysis of PTGES3 using anti-PTGES3 antibody (A04136-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HEPG2 whole cell lysates, <br>
Lane 4: human COLO-320 whole cell lysates, <br>
Lane 5: human Raji whole cell lysates, <br>
Lane 6: human HEK293 whole cell lysates, <br>
Lane 7: human K562 whole cell lysates, <br>
Lane 8: human THP-1 whole cell lysates, <br>
Lane 9: rat PC-12 whole cell lysates, <br>
Lane 10: mouse NIH/3T3 whole cell lysates, <br>
Lane 11: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES3 antigen affinity purified polyclonal antibody (Catalog # A04136-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES3 at approximately 23KD. The expected band size for PTGES3 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04136-1-ptges3-primary-antibodies-fcm-testing-2.pngAnti-p23/PTGES3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-PTGES3 antibody (A04136-1).<br>Overlay histogram showing A549 cells stained with A04136-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGES3 Antibody (A04136-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04136-1-ptges3-primary-antibodies-if-testing-3.jpgAnti-p23/PTGES3 Antibody Picoband® IF analysis of PTGES3 using anti-PTGES3 antibody (A04136-1). <br>
PTGES3 was detected in immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PTGES3 Antibody (A04136-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-radixin-rdx-picoband-trade-antibody-a01926-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01926-1-RDX-primary-antibodies-WB-testing-1.jpgAnti-Radixin/RDX Antibody Picoband® Western blot analysis of RDX using anti-RDX antibody (A01926-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human PC-3 whole cell lysates, <br>
Lane 7: human HepG2 whole cell lysates, <br>
Lane 8: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDX antigen affinity purified polyclonal antibody (Catalog # A01926-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDX at approximately 88KD. The expected band size for RDX is at 69KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01926-1-RDX-primary-antibodies-WB-testing-2.jpgAnti-Radixin/RDX Antibody Picoband® Western blot analysis of RDX using anti-RDX antibody (A01926-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: mouse lung tissue lysates, <br>
Lane 4: mouse liver tissue lysates, <br>
Lane 5: mouse brain tissue lysates, <br>
Lane 6: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDX antigen affinity purified polyclonal antibody (Catalog # A01926-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDX at approximately 88KD. The expected band size for RDX is at 69KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01926-1-rdx-primary-antibodies-if-testing-3.jpgAnti-Radixin/RDX Antibody Picoband® IF analysis of RDX using anti-RDX antibody (A01926-1). <br>
RDX was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RDX Antibody (A01926-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01926-1-rdx-primary-antibodies-fc-testing-4.jpgAnti-Radixin/RDX Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RDX antibody (A01926-1). <br>Overlay histogram showing K562 cells stained with A01926-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RDX Antibody (A01926-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rpl13a-picoband-trade-antibody-a03571-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03571-1-rpl13a-primary-antibodies-wb-testing-1.jpgAnti-RPL13A Antibody Picoband® Western blot analysis of RPL13A using anti-RPL13A antibody (A03571-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human RT4 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human 293T whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL13A antigen affinity purified polyclonal antibody (Catalog # A03571-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL13A at approximately 24 kDa. The expected band size for RPL13A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03571-1-RPL13A-primary-antibodies-IHC-testing-2.jpgAnti-RPL13A Antibody Picoband® IHC analysis of RPL13A using anti-RPL13A antibody (A03571-1). <br>RPL13A was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPL13A Antibody (A03571-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03571-1-RPL13A-primary-antibodies-IHC-testing-3.jpgAnti-RPL13A Antibody Picoband® IHC analysis of RPL13A using anti-RPL13A antibody (A03571-1). <br>RPL13A was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPL13A Antibody (A03571-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03571-1-RPL13A-primary-antibodies-IHC-testing-4.jpgAnti-RPL13A Antibody Picoband® IHC analysis of RPL13A using anti-RPL13A antibody (A03571-1). <br>RPL13A was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPL13A Antibody (A03571-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-skp1-picoband-trade-antibody-a00476-2-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-2-skp1-primary-antibodies-wb-testing-1.jpgAnti-SKP1 Antibody Picoband® Western blot analysis of SKP1 using anti-SKP1 antibody (A00476-2). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human T47D whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: rat RH35 whole cell lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 7: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP1 antigen affinity purified polyclonal antibody (A00476-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKP1 at approximately 19 kDa. The expected band size for SKP1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-2-skp1-primary-antibodies-ihc-testing-3.jpgAnti-SKP1 Antibody Picoband®IHC analysis of SKP1 using anti-SKP1 antibody (A00476-2). <br>SKP1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKP1 Antibody (A00476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-2-skp1-primary-antibodies-ihc-testing-2.jpgAnti-SKP1 Antibody Picoband® IHC analysis of SKP1 using anti-SKP1 antibody (A00476-2). <br>
SKP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKP1 Antibody (A00476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-2-skp1-primary-antibodies-if-testing-3.jpgAnti-SKP1 Antibody Picoband® IF analysis of SKP1 using anti-SKP1 antibody (A00476-2) and anti-Tubulin Alpha antibody (M03989-3).<br>
SKP1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SKP1 Antibody (A00476-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00476-2-skp1-primary-antibodies-fcm-testing-4.jpgAnti-SKP1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SKP1 antibody (A00476-2). <br>
Overlay histogram showing PC-3 cells stained with A00476-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKP1 Antibody (A00476-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-suz12-picoband-trade-antibody-a00583-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00583-1-SUZ12-primary-antibodies-WB-testing-1.jpgAnti-SUZ12 Antibody Picoband® Western blot analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human HL-60 whole cell lysates, <br>Lane 2: rat C6 whole cell lysates, <br>Lane 3: mouse SP20 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12 antigen affinity purified polyclonal antibody (Catalog # A00583-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUZ12 at approximately 90KD. The expected band size for SUZ12 is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00583-1-SUZ12-primary-antibodies-IHC-testing-2.jpgAnti-SUZ12 Antibody Picoband® IHC analysis of SUZ12 using anti-SUZ12 antibody (A00583-1). <br>SUZ12 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SUZ12 Antibody (A00583-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00583-1-suz12-primary-antibodies-if-testing-3.jpgAnti-SUZ12 Antibody Picoband® IF analysis of SUZ12 using anti-SUZ12 antibody (A00583-1) and anti-Beta Tubulin antibody (M01857-3).<br>
SUZ12 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/mL rabbit anti-SUZ12 Antibody (A00583-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-tafazzin-taz-picoband-trade-antibody-a00933-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00933-taz-primary-antibodies-wb-testing-1.jpgAnti-Tafazzin/TAZ Antibody Picoband®Western blot analysis of TAZ using anti-TAZ antibody (A00933). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAZ antigen affinity purified polyclonal antibody (A00933) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAZ at approximately 33 kDa. The expected band size for TAZ is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00933-taz-primary-antibodies-ihc-testing-1.jpgAnti-Tafazzin/TAZ Antibody Picoband®IHC analysis of TAZ using anti-TAZ antibody (A00933). <br>
TAZ was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAZ Antibody (A00933) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd90-thy1-picoband-trade-antibody-a01818-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01818-1-thy1-primary-antibodies-wb-testing-1_1.jpgAnti-CD90/Thy1 Antibody Picoband®Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1). <br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: rat thymus tissue lysates, <br>
Lane 4: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01818-1-thy1-primary-antibodies-ihc-testing-1.jpgAnti-CD90/Thy1 Antibody Picoband®IHC analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1). <br>
CD90/Thy1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD90/Thy1 Antibody (A01818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-timp1-picoband-trade-antibody-a00561-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00561-1-Timp-1-primary-antibodies-WB-testing-1.jpgAnti-TIMP1 Antibody Picoband® Western blot analysis of Timp-1 using anti-Timp-1 antibody (A00561-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human SK-OV-3 whole cell lysates, <br>
Lane 2: human 22RV1 whole cell lysates, <br>
Lane 3: rat ovarian tissue lysates, <br>
Lane 4: mouse ovarian tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Timp-1 antigen affinity purified polyclonal antibody (Catalog # A00561-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Timp-1 at approximately 26KD. The expected band size for Timp-1 is at 23KD.
https://www.bosterbio.com/products/primary-antibodies/anti-osteoprotegerin-tnfrsf11b-picoband-trade-antibody-a00863-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-wb-testing-1.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® Western blot analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates,<br>
Lane 2: monkey COS-7 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF11B antigen affinity purified polyclonal antibody (Catalog # A00863) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF11B at approximately 55 kDa. The expected band size for TNFRSF11B is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-fnut-12-1591070-g004.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband®Effects of WGE on bone OPG, RANKL and Runx2 proteins and mRNA expression in STZ-induced DM rats. Immunohistochemical staining of OPG, RANKL and Runx2 proteins (Top) (400×); (A–C) Percentage of positive staining of OPG, RANKL, and Runx2 protein; (D–F) OPG, RANKL , and Runx2 mRNA expression level. Values are the mean ± SD ( n = 10). ## p < 0.01 vs. NC group; ** p < 0.01 vs. DM group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1591070/full'>40529421</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-2.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-3.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human endometrial carcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-4.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-5.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-6.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-7.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-8.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863).
TNFRSF11B was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00863-TNFRSF11B-primary-antibodies-IHC-testing-9.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863).
TNFRSF11B was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-10.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-11.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-12.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-13.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-16.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human Cholangiocarcinoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-17.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-15.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-18.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-19.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-14.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-20.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-21.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-22.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br> TNFRSF11B was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-fc-testing-24.pngAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TNFRSF11B antibody (A00863). <br>Overlay histogram showing U20S cells stained with A00863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF11B Antibody (A00863,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00863-tnfrsf11b-primary-antibodies-ihc-testing-23.jpgAnti-Osteoprotegerin/TNFRSF11B Antibody Picoband® IHC analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). <br>
TNFRSF11B was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFRSF11B Antibody (A00863) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-topoisomerase-ii-alpha-top2a-picoband-trade-antibody-a00953-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00953-TOP2A-primary-antibodies-WB-testing-1.jpgAnti-Topoisomerase II alpha/TOP2A Antibody Picoband® Western blot analysis of TOP2A using anti-TOP2A antibody (A00953). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human A549 whole cell lysates, <br>Lane 2: human U2OS whole cell lysates, <br>Lane 3: human HEK293 whole cell lysates, <br>Lane 4: human K562 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP2A antigen affinity purified polyclonal antibody (Catalog # A00953) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOP2A at approximately 174KD. The expected band size for TOP2A is at 174KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00953-TOP2A-primary-antibodies-IHC-testing-2.jpgAnti-Topoisomerase II alpha/TOP2A Antibody Picoband® IHC analysis of TOP2A using anti-TOP2A antibody (A00953). <br>TOP2A was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TOP2A Antibody (A00953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00953-TOP2A-primary-antibodies-IHC-testing-3.jpgAnti-Topoisomerase II alpha/TOP2A Antibody Picoband® IHC analysis of TOP2A using anti-TOP2A antibody (A00953). <br> TOP2A was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TOP2A Antibody (A00953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A00953-TOP2A-primary-antibodies-FC-testing-4.pngAnti-Topoisomerase II alpha/TOP2A Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TOP2A antibody (A00953). <br>Overlay histogram showing 293T cells stained with A00953 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOP2A Antibody (A00953,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00953-top2a-primary-antibodies-if-testing-5.jpgAnti-Topoisomerase II alpha/TOP2A Antibody Picoband® IF analysis of TOP2A using anti-TOP2A antibody (A00953). <br>
TOP2A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TOP2A Antibody (A00953) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ube2i-ubc9-picoband-trade-antibody-a02295-1-boster.html2026-03-24T05:25:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-wb-testing-1_1_1.jpgAnti-UBE2I/UBC9 Antibody Picoband® Western blot analysis of UBC9/UBE2I using anti-UBC9/UBE2I antibody (A02295-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: rat kidney tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC9/UBE2I antigen affinity purified polyclonal antibody (A02295-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC9/UBE2I at approximately 18 kDa. The expected band size for UBC9/UBE2I is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-2.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-3.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-4.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-5.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-6.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-7.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-ihc-testing-8.jpgAnti-UBE2I/UBC9 Antibody Picoband® IHC analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-if-testing-9.jpgAnti-UBE2I/UBC9 Antibody Picoband® IF analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (A02295-1). <br>
UBE2I/UBC9 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-UBE2I/UBC9 Antibody (A02295-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02295-1-ube2i-primary-antibodies-fcm-testing-10.pngAnti-UBE2I/UBC9 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-UBE2I/UBC9 antibody (A02295-1). <br>Overlay histogram showing A431 cells stained with A02295-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2I/UBC9 Antibody (A02295-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mvp-picoband-trade-antibody-monoclonal-m00642-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-mvp-primary-antibodies-wb-testing-1.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) Western blot analysis of MVP using anti-MVP antibody (M00642-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 tissue lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: human Hela whole cell lysates, <br>
Lane 6: human SW620 whole cell lysates. <br>
Lane 7: rat RH35 whole cell lysates. <br>
Lane 8: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MVP antigen affinity purified polyclonal antibody (Catalog # M00642-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MVP at approximately 100-110KD. The expected band size for MVP is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-mvp-primary-antibodies-if-testing-10.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IF analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-MVP Antibody (M00642-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-2.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-3.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-4.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12)
IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-5.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-6.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-ihc-testing-7.jpgAnti-MVP Antibody Picoband® (monoclonal, 8B12) IHC analysis of MVP using anti-MVP antibody (M00642-1). <br>
MVP was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-MVP Antibody (M00642-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-fc-testing-8.pngAnti-MVP Antibody Picoband® (monoclonal, 8B12) Flow Cytometry analysis of A549 cells using anti-MVP antibody (A00043-1). <br>Overlay histogram showing A549 cells stained with A00043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MVP Antibody (A00043-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00642-1-_mvp_-primary-antibodies-fc-testing-9.pngAnti-MVP Antibody Picoband® (monoclonal, 8B12) Flow Cytometry analysis of A431 cells using anti-MVP antibody (A00043-1). <br>Overlay histogram showing A431 cells stained with A00043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MVP Antibody (A00043-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh2-picoband-trade-antibody-monoclonal-m00546-1-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-1-aldh2-primary-antibodies-wb-testing-1.jpgAnti-ALDH2 Antibody Picoband® (monoclonal, 5G7) Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 tissue lysates, <br>
Lane 2: human placenta whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human SHG-44 whole cell lysates, <br>
Lane 5: human THP-1 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ALDH2 antigen affinity purified polyclonal antibody (Catalog # M00546-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ALDH2 at approximately 56KD. The expected band size for ALDH2 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-1-aldh2-primary-antibodies-wb-testing-2.jpgAnti-ALDH2 Antibody Picoband® (monoclonal, 5G7)
Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat kidney whole cell lysates, <br>
Lane 3: rat heart whole cell lysates, <br>
Lane 4: mouse liver whole cell lysates, <br>
Lane 5: mouse kidney whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ALDH2 antigen affinity purified polyclonal antibody (Catalog # M00546-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ALDH2 at approximately 56KD. The expected band size for ALDH2 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-1-aldh2-primary-antibodies-fc-testing-3.pngAnti-ALDH2 Antibody Picoband® (monoclonal, 5G7) Flow Cytometry analysis of A549 cells using anti-ALDH2 antibody (M00546-1). <br>Overlay histogram showing A549 cells stained with M00546-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ALDH2 Antibody (M00546-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-1-aldh2-primary-antibodies-if-testing-4.jpgAnti-ALDH2 Antibody Picoband® (monoclonal, 5G7) IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-1). <br>
ALDH2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ALDH2 Antibody (M00546-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd79a-picoband-trade-antibody-monoclonal-m01047-3-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01047-3-cd79a-primary-antibodies-wb-testing-1.jpgAnti-CD79a Antibody Picoband® (monoclonal, 4G4) Western blot analysis of CD79A using anti ZO-1 antibody (M01047-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Raji tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD79A antigen affinity purified polyclonal antibody (Catalog # M01047-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD79A at approximately 44KD. The expected band size for CD79A is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01047-3-cd79a-primary-antibodies-ihc-testing-2.jpgAnti-CD79a Antibody Picoband® (monoclonal, 4G4) IHC analysis of CD79A using anti-CD79A antibody (M01047-3). <br>
CD79A was detected in paraffin-embedded section of human tonsil cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79A Antibody (M01047-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tjp1-picoband-trade-antibody-monoclonal-m00860-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-tjp1-primary-antibodies-wb-testing-1.jpgAnti-TJP1 Antibody Picoband® (monoclonal, 3E12) Western blot analysis of TJP1 using anti-TJP1 antibody (M00860). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates, <br>
Lane 2: human U20S whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human T47D whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TJP1 antigen affinity purified monoclonal antibody (Catalog # M00860) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 195 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-zo-1-primary-antibodies-ihc-testing-2.jpgAnti-TJP1 Antibody Picoband® (monoclonal, 3E12) IHC analysis of TJP1 using anti-TJP1 antibody (M00860). <br>
TJP1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-TJP1 Antibody (M00860) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogenhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-zo-1-primary-antibodies-ihc-testing-3.jpgAnti-TJP1 Antibody Picoband® (monoclonal, 3E12) IHC analysis of TJP1 using anti-TJP1 antibody (M00860). <br>
TJP1 was detected in paraffin-embedded section of human kidney cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-TJP1 Antibody (M00860) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-zo-1-primary-antibodies-fc-testing-4.pngAnti-TJP1 Antibody Picoband® (monoclonal, 3E12) Flow Cytometry analysis of hela cells using anti-TJP1 antibody (M00860). <br>Overlay histogram showing hela cells stained with M00860 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TJP1 Antibody (M00860, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-tjp1-primary-antibodies-if-testing-5.jpgAnti-TJP1 Antibody Picoband® (monoclonal, 3E12) IF analysis of TJP1 using anti-TJP1 antibody (M00860). <br>
TJP1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-TJP1 Antibody (M00860) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-homer3-picoband-trade-antibody-monoclonal-m09145-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09145-homer3-primary-antibodies-wb-testing-1.jpgAnti-HOMER3 Antibody Picoband® (monoclonal, 9G13) Western blot analysis of HOMER3 using anti-HOMER3 antibody (M09145). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U-87MG tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IL-32 antigen affinity purified polyclonal antibody (Catalog # M09145) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HOMER3 at approximately 45KD. The expected band size for HOMER3 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09145-homer3-primary-antibodies-fc-testing-2.pngAnti-HOMER3 Antibody Picoband® (monoclonal, 9G13) Flow Cytometry analysis of U937 cells using anti-HOMER3 antibody M09145). <br>Overlay histogram showing U937 cells stained with M09145 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HOMER3 Antibody (M09145, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tuberin-picoband-trade-antibody-monoclonal-m00229-1-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00229-1-tsc2-primary-antibodies-wb-testing-1_1.jpgAnti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) Western blot analysis of TSC2 using anti-TSC2 antibody (M00229-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 tissue lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TSC2 antigen affinity purified polyclonal antibody (Catalog # M00229-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TSC2 at approximately 280KD. The expected band size for TSC2 is at 201KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00229-1-tuberin-primary-antibodies-ihc-testing-2.jpgAnti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) IHC analysis of TSC2 using anti-TSC2 antibody (M00229-1). <br>
TSC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-TSC2 Antibody (M00229-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00229-1-tuberin-primary-antibodies-fcm-testing-3.jpgAnti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) Flow Cytometry analysis of A549 cells using anti-TSC2 antibody (M00229-1). <br>Overlay histogram showing A549 cells stained with M00229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TSC2 Antibody (M00229-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mttfa-picoband-trade-antibody-monoclonal-m01119-1-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-1-tfam-primary-antibodies-wb-testing-1.jpgAnti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) Western blot analysis of TFAM using anti ZO-1 antibody (M01119-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TFAM antigen affinity purified polyclonal antibody (Catalog # M01119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TFAM at approximately 40KD. The expected band size for TFAM is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-1-tfam-primary-antibodies-ihc-testing-2.jpgAnti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) IHC analysis of TFAM using anti-TFAM antibody (M01119-1). <br>
TFAM was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-TFAM Antibody (M01119-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-1-tfam-primary-antibodies-ihc-testing-3.jpgAnti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) IHC analysis of TFAM using anti-TFAM antibody (M01119-1). <br>
TFAM was detected in paraffin-embedded section of human Breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-TFAM Antibody (M01119-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-1-tfam-primary-antibodies-fc-testing-4.pngAnti-mtTFA TFAM Antibody Picoband® (monoclonal, 4D9) Flow Cytometry analysis of CACO-2 cells using anti-FAM antibody (M01119-1). <br>Overlay histogram showing CACO-2 cells stained with M01119-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FAM Antibody (M01119-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dck-picoband-trade-antibody-monoclonal-m01655-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01655-dck-primary-antibodies-wb-testing-1.jpgAnti-DCK Antibody Picoband® (monoclonal, 3G10) Western blot analysis of DCK using anti ZO-1 antibody (M01655). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human Jurkat whole cell lysates, <br>
Lane 5: human Raji whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DCK antigen affinity purified polyclonal antibody (Catalog # M01655) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DCK at approximately 30KD. The expected band size for DCK is at 30KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01655-dck-primary-antibodies-ihc-testing-2.jpgAnti-DCK Antibody Picoband® (monoclonal, 3G10) IHC analysis of DCK using anti-DCK antibody (M01655). <br>
DCK was detected in paraffin-embedded section of human tonsil cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DCK Antibody (M01655) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01655-dck-primary-antibodies-if-testing-3.jpgAnti-DCK Antibody Picoband® (monoclonal, 3G10) IF analysis of DCK using anti-DCK antibody (M01655). <br>
DCK was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-DCK Antibody (M01655) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01655-dck-primary-antibodies-fc-testing-4.pngAnti-DCK Antibody Picoband® (monoclonal, 3G10) Flow Cytometry analysis of 293T cells using anti-DCK antibody (M01655). <br>Overlay histogram showing 293T cells stained with M01655 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-DCK Antibody (M01655, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-clpx-picoband-trade-antibody-monoclonal-m00978-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00978-clpx-primary-antibodies-wb-testing-1.jpgAnti-CLPX Antibody Picoband® (monoclonal, 11C11) Western blot analysis of CLPX using anti ZO-1 antibody (M00978). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Raji tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CLPX antigen affinity purified polyclonal antibody (Catalog # M00978) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CLPX at approximately 69KD. The expected band size for CLPX is at 69KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00978-clpx-primary-antibodies-if-testing-2.jpgAnti-CLPX Antibody Picoband® (monoclonal, 11C11) IF analysis of CLPX using anti-CLPX antibody (M00978). <br>
CLPX was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CLPX Antibody (M00978) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-tubulin-picoband-trade-antibody-monoclonal-m05613-4-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin-beta-primary-antibodies-wb-testing-1.jpgAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat lung tissue lysates,<br>
Lane 6: mouse brain tissue lysates,<br>
Lane 6: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tubulin beta antigen affinity purified monoclonal antibody (Catalog # M05613-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tubulin beta at approximately 55 kDa. The expected band size for Tubulin beta is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin-beta-primary-antibodies-if-testing-2.jpgAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) IF analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4) and anti-FOSB antibody (A01569-1). <br>
Tubulin beta was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Tubulin beta Antibody (M05613-4) and rabbit anti-FOSB antibody (A01569-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) and DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin_beta-primary-antibodies-if-testing-3.jpgAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) IF analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4). <br>
Tubulin beta was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin_beta-primary-antibodies-if-testing-4.jpgAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) IF analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4). <br>
Tubulin beta was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin_beta-primary-antibodies-fc-testing-5.pngAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) Flow Cytometry analysis of U937 cells using anti-Tubulin beta antibody M05613-4). <br>Overlay histogram showing U937 cells stained with M05613-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Tubulin beta Antibody (M05613-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin_beta-primary-antibodies-fc-testing-6.pngAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) Flow Cytometry analysis of HEPA1-6 cells using anti-Tubulin beta antibody M05613-4). <br>Overlay histogram showing HEPA1-6 cells stained with M05613-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Tubulin beta Antibody (M05613-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-4-tubulin_beta-primary-antibodies-wb-testing-7.jpgAnti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (M05613-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat lung tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tubulin beta antigen affinity purified monoclonal antibody (M05613-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for Tubulin beta at approximately 50 kDa. The expected band size for Tubulin beta is at 50 kDa.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd5-picoband-trade-antibody-monoclonal-m00480-2-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00480-2-cd5-primary-antibodies-wb-testing-1.jpgAnti-CD5 Antibody Picoband® (monoclonal, 4E2) Western blot analysis of CD5 using anti-CD5 antibody (M00480-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Jurkat tissue lysates, <br>
Lane 2: human CCRM-CEM whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD5 antigen affinity purified polyclonal antibody (Catalog # M00480-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00480-2-cd5-primary-antibodies-ihc-testing-2.jpgAnti-CD5 Antibody Picoband® (monoclonal, 4E2)
IHC analysis of CD5 using anti-CD5 antibody (M00480-2). <br>
CD5 was detected in paraffin-embedded section of human tonsil cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD5 Antibody (M00480-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd22-picoband-trade-antibody-monoclonal-m01572-2-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01572-2-cd22-primary-antibodies-wb-testing-1.jpgAnti-CD22 Antibody Picoband® (monoclonal, 5E7) Western blot analysis of CD22 using anti ZO-1 antibody (M01572-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Raji tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD22 antigen affinity purified polyclonal antibody (Catalog # M01572-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rp2-picoband-trade-antibody-monoclonal-m01923boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01923-rp2-primary-antibodies-wb-testing-1.jpgAnti-RP2 Antibody Picoband® (monoclonal, 3D7) Western blot analysis of RP2 using anti ZO-1 antibody (M01923). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 tissue lysates, <br>
Lane 2: human Jurkat, whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RP2 antigen affinity purified polyclonal antibody (Catalog # M01923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01923-rp2-primary-antibodies-if-testing-2.jpgAnti-RP2 Antibody Picoband® (monoclonal, 3D7) IF analysis of RP2 using anti-RP2 antibody (M01923). <br>
RP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RP2 Antibody (M01923) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01923-rp2-primary-antibodies-fc-testing-3.pngAnti-RP2 Antibody Picoband® (monoclonal, 3D7) Flow Cytometry analysis of 293T cells using anti-RP2 antibody M01923). <br>Overlay histogram showing 293T cells stained with M01923 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RP2 Antibody (M01923, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il32-picoband-trade-antibody-monoclonal-m03286-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03286-il32-primary-antibodies-elisa-testing-1.jpgAnti-IL32 Antibody (monoclonal, 2I2) Sandwich ELISA - Recombinant human IL32 protein standard curve.<br>
Use in combination with reagents from Human IL32 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1599).
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mpi-picoband-trade-antibody-monoclonal-m00175-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00175-mpi-primary-antibodies-wb-testing-1_2.jpgAnti-MPI Antibody Picoband® (monoclonal, 11I4) Western blot analysis of MPI using anti-MPI antibody (M00175). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Caco-2 whole cell lysates<br>
Lane 2: human Hela whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MPI antigen affinity purified monoclonal antibody (Catalog # M00175) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MPI at approximately 47KD. The expected band size for MPI is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00175-mpi-primary-antibodies-if-testing-2.jpgAnti-MPI Antibody Picoband® (monoclonal, 11I4) IF analysis of MPI using anti-MPI antibody (M00175). <br>
MPI was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-MPI Antibody (M00175) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il6-picoband-trade-antibody-monoclonal-m00102-2-boster.html2026-03-24T05:25:53+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sae2-uba2-picoband-trade-antibody-monoclonal-m03816-2-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03816-2-uba2-primary-antibodies-wb-testing-1.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5B13) Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2antibody (M03816-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Raji whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human SW579 whole cell lysates, <br>
Lane 5: human CCRF-CEM whole cell lysates, <br>
Lane 6: rat PC-12 whole cell lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SAE2/UBA2 antigen affinity purified monoclonal antibody (Catalog # M03816-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 90KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03816-2-uba2-primary-antibodies-ihc-testing-2.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5B13) IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (M03816-2). <br>
SAE2/UBA2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SAE2/UBA2 Antibody (M03816-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03816-2-uba2-primary-antibodies-ihc-testing-3.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5B13) IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (M03816-2). <br>
SAE2/UBA2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SAE2/UBA2 Antibody (M03816-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03816-2-uba2-primary-antibodies-ihc-testing-4.jpgAnti-SAE2/UBA2 Antibody Picoband® (monoclonal, 5B13) IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (M03816-2). <br>
SAE2/UBA2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SAE2/UBA2 Antibody (M03816-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/picokine-elisa-kits/human-aplp1-picokine-elisa-kit-ek2103-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2103.pngHuman APLP1 ELISA Kit PicoKine®Human APLP1 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/picokine-elisa-kits/human-qpct-picokine-elisa-kit-ek2104-boster.html2026-03-24T05:25:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2104.pngHuman QPCT ELISA Kit PicoKine®Human QPCT PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/human-pcolce-picokine-elisa-kit-ek2105-boster.html2026-03-25T05:23:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2105.pngHuman PCOLCE ELISA Kit PicoKine®Human PCOLCE PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/mouse-pcolce-picokine-elisa-kit-ek2106-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2106.pngMouse PCOLCE ELISA Kit PicoKine®Mouse PCOLCE PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/mouse-klkb1-picokine-elisa-kit-ek2107-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2107_1.pngMouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®Mouse KLKB1 PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/human-antxr2-picokine-elisa-kit-ek2108-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2108.pngHuman ANTXR2 ELISA Kit PicoKine®Human ANTXR2 PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/human-cant1-picokine-elisa-kit-ek2109-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2109.pngHuman CANT1 ELISA Kit PicoKine®Human CANT1 PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/human-prelp-picokine-elisa-kit-ek2110-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2110.jpgHuman PRELP/Prolargin ELISA Kit PicoKine®Human PRELP PicoKine ELISA Kit
https://www.bosterbio.com/picokine-elisa-kits/mouse-cd44-picokine-trade-elisa-kit-ek1419-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1419.jpgMouse CD44 ELISA Kit PicoKine®Mouse CD44 PicoKine ELISA Kit standard curve
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-eif4e-antibody-dz00135-boster.html2026-03-24T05:25:54+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-f8-antibody-dz00367-boster.html2026-03-24T05:25:54+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-cheerio-filamin-antibody-dz00502-boster.html2026-03-24T05:25:54+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/anti-abcg8-antibody-a01482-2-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01482-2-ABCG8-primary-antibodies-WB-testing-1_1.jpgAnti-ABCG8 Antibody Picoband® Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates<br>
Lane 2: rat small intestine tissue lysates<br>
Lane 3: mouse liver tissue lysates<br>
Lane 4: rat RH35 whole cell lysates <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (Catalog # A01482-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG8 at approximately 76KD. The expected band size for ABCG8 is at 76KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01482-2-ABCG8-primary-antibodies-FC-testing-2.jpgAnti-ABCG8 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ABCG8 antibody (A01482-2).<br>Overlay histogram showing HepG2 cells stained with A01482-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG8 Antibody (A01482-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A01482-2-ABCG8-primary-antibodies-FC-testing-3.jpgAnti-ABCG8 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-ABCG8 antibody (A01482-2).<br>Overlay histogram showing RH35 cells stained with A01482-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCG8 Antibody (A01482-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01482-2-abcg8-primary-antibodies-if-testing-4.jpgAnti-ABCG8 Antibody Picoband® IF analysis of ABCG8 using anti-ABCG8 antibody (A01482-2). <br>
ABCG8 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ABCG8 Antibody (A01482-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-acetyl-coenzyme-a-carboxylase-acabc-picoband-trade-antibody-a03668-2-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03668-2-ACACB-primary-antibodies-WB-testing-1_1.jpgAnti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® Western blot analysis of ACACB using anti-ACACB antibody (A03668-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat skeletal muscle tissue lysates<br>Lane 2: mouse skeletal muscle tissue lysates<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACB antigen affinity purified polyclonal antibody (Catalog # A03668-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACB at approximately 277KD. The expected band size for ACACB is at 277KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03668-2-gr6.jpgAnti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband®Expression of the lipid metabolism regulator PPARβ and downstream effectors (CPT1b, FAS, and ACC2). Protein levels in obese rats under hypoxic conditions, with hypoxic training, and with regulated miR-122 expression were determined using Western blot analysis. OE, obese rats with miR-122 overexpression and hypoxic training; IE, obese rats with miR-122 depletion and hypoxic training; CE, obese rats with hypoxic training only; H, obese sedentary rats without regulation of miR-122 expression. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 vs rats in group H; # p < 0.05, ## p < 0.01 vs rats in group CE; & p < 0.05, && p < 0.01 vs rats in group OE (1-way analysis of variance). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10906430/&orig_host=www.ncbi.nlm.nih.gov'>38434053</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03668-2-acacb-primary-antibodies-ihc-testing-2_1.jpgAnti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® IHC analysis of ACACB using anti-ACACB antibody (A03668-2).<br> ACACB was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ACACB Antibody (A03668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A03668-2-ACACB-primary-antibodies-FC-testing-3_1.jpgAnti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ACACB antibody (A03668-2).<br> Overlay histogram showing HL-60 cells stained with A03668-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACACB Antibody (A03668-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-facl4-acsl4-picoband-trade-antibody-a04372-2-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-facl4-primary-antibodies-ihc-testing-4.jpgAnti-FACL4/ACSL4 Antibody Picoband®IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (A04372-2). <br>ACSL4/FACL4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACSL4/FACL4 Antibody (A04372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-wb-testing-1.jpgAnti-FACL4/ACSL4 Antibody Picoband® Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-ihc-testing-2.jpgAnti-FACL4/ACSL4 Antibody Picoband® IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). <br>
FACL4/ACSL4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FACL4/ACSL4 Antibody (A04372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-ihc-testing-3.jpgAnti-FACL4/ACSL4 Antibody Picoband® IHC analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). <br>
FACL4/ACSL4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FACL4/ACSL4 Antibody (A04372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-if-testing-4.jpgAnti-FACL4/ACSL4 Antibody Picoband® IF analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-2). <br>
FACL4/ACSL4 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FACL4/ACSL4 Antibody (A04372-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-fcm-testing-5.jpgAnti-FACL4/ACSL4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-FACL4/ACSL4 antibody (A04372-2). <br>
Overlay histogram showing HepG2 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FACL4/ACSL4 Antibody (A04372-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-2-acsl4-primary-antibodies-fcm-testing-6_1.jpgAnti-FACL4/ACSL4 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-FACL4/ACSL4 antibody (A04372-2). <br>
Overlay histogram showing U87 cells stained with A04372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FACL4/ACSL4 Antibody (A04372-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-acetyl-coenzyme-a-carboxylase-acabc-picoband-trade-antibody-a02381-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-wb-testing-1.jpgAnti-APPL/APPL1 Antibody Picoband® Western blot analysis of APPL1 using anti-APPL1 antibody (A02381). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPL1 antigen affinity purified polyclonal antibody (Catalog # A02381) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPL1 at approximately 85 kDa. The expected band size for APPL1 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-fc-testing-6.pngAnti-APPL/APPL1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-APPL1 antibody (A02381). <br>Overlay histogram showing A549 cells stained with A02381 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APPL1 Antibody (A02381, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-ihc-testing-2.jpgAnti-APPL/APPL1 Antibody Picoband® IHC analysis of APPL1 using anti-APPL1 antibody (A02381).<br>
APPL1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APPL1 Antibody (A02381) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-ihc-testing-3.jpgAnti-APPL/APPL1 Antibody Picoband® IHC analysis of APPL1 using anti-APPL1 antibody (A02381).<br>
APPL1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APPL1 Antibody (A02381) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-ihc-testing-4.jpgAnti-APPL/APPL1 Antibody Picoband® IHC analysis of APPL1 using anti-APPL1 antibody (A02381).<br>
APPL1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APPL1 Antibody (A02381) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02381-appl1-primary-antibodies-if-testing-5.jpgAnti-APPL/APPL1 Antibody Picoband® IF analysis of APPL1 using anti-APPL1 antibody (A02381). <br>
APPL1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-APPL1 Antibody (A02381) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-acetyl-coenzyme-a-carboxylase-acabc-picoband-trade-antibody-a00456-2-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00456-2-b2m-primary-antibodies-wb-testing-1.jpgAnti-beta 2 Microglobulin/B2m Antibody Picoband® Western blot analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: rat spleen tissue lysates, <br>
Lane 4: rat stomach tissue lysates, <br>
Lane 5: rat small intestine tissue lysates, <br>
Lane 6: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta 2 Microglobulin/B2m antigen affinity purified polyclonal antibody (Catalog # A00456-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta 2 Microglobulin/B2m at approximately 13KD. The expected band size for Beta 2 Microglobulin/B2m is at 13KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00456-2-b2m-primary-antibodies-if-testing-2.jpgAnti-beta 2 Microglobulin/B2m Antibody Picoband® IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2). <br>
Beta 2 Microglobulin/B2m was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00456-2-b2m-primary-antibodies-if-testing-3.jpgAnti-beta 2 Microglobulin/B2m Antibody Picoband® IF analysis of Beta 2 Microglobulin/B2m using anti-Beta 2 Microglobulin/B2m antibody (A00456-2). <br>
Beta 2 Microglobulin/B2m was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00456-2-b2m-primary-antibodies-fcm-testing-4.pngAnti-beta 2 Microglobulin/B2m Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-Beta 2 Microglobulin/B2m antibody (A00456-2). <br>Overlay histogram showing RH35 cells stained with A00456-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta 2 Microglobulin/B2m Antibody (A00456-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-c9orf72-antibody-picoband-a00419-1-boster.html2026-03-24T05:25:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00419-1-c9orf72-primary-antibodies-wb-testing-1.jpgAnti-C9ORF72 Antibody Picoband® Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human U2OS whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: human Hela whole cell lysates, <br> Lane 6: human A431 whole cell lysates, <br> Lane 7: human A549 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00419-1-c9orf72-primary-antibodies-wb-testing-2.jpgAnti-C9ORF72 Antibody Picoband® Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat C6 whole cell lysates, <br> Lane 4: mouse liver tissue lysates, <br> Lane 5: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00419-1-c9orf72-primary-antibodies-fc-testing-3.pngAnti-C9ORF72 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-C9ORF72 antibody (A00419-1). <br> Overlay histogram showing U20S cells stained with A00419-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C9ORF72 Antibody (A00419-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ca12-picoband-trade-antibody-a04063-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04063-1-ca12-primary-antibodies-wb-testing-1_1.jpgAnti-CA12 Antibody Picoband® Western blot analysis of CA12 using anti-CA12 antibody (A04063-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA12 antigen affinity purified polyclonal antibody (Catalog # A04063-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA12 at approximately 45 kDa. The expected band size for CA12 is at 39 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-acetyl-coenzyme-a-carboxylase-acabc-picoband-trade-antibody-a00323-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00323-cd1d-primary-antibodies-wb-testing-1.jpgAnti-CD1D Antibody Picoband® Western blot analysis of CD1D using anti-CD1D antibody (A00323). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: human THP-1 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1D antigen affinity purified polyclonal antibody (Catalog # A00323) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1D at approximately 55KD. The expected band size for CD1D is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00323-cd1d-primary-antibodies-fc-testing-2.pngAnti-CD1D Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-CD1D antibody (A00323). <br> Overlay histogram showing U20S cells stained with A00323 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD1D Antibody (A00323, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd3-epsilon-cd3e-antibody-picoband-a02765-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-1-cd3e-primary-antibodies-wb-testing-1_1.jpgAnti-CD3 epsilon/Cd3e Antibody Picoband® Western blot analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates,<br>
Lane 2: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon/Cd3e antigen affinity purified polyclonal antibody (Catalog # A02765-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 epsilon/Cd3e at approximately 23 kDa. The expected band size for CD3 epsilon/Cd3e is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-1-cd3e-primary-antibodies-fcm-testing-5.jpgAnti-CD3 epsilon/Cd3e Antibody Picoband® Flow Cytometry analysis of mouse spleen tissues using anti-CD3 epsilon/Cd3e antibody (A02765-1). <br>Overlay histogram showing mouse spleen tissues stained with A02765-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD3 epsilon/Cd3e Antibody (A02765-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-1-cd3e-primary-antibodies-ihc-testing-2_1.jpgAnti-CD3 epsilon/Cd3e Antibody Picoband® IHC analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). <br>
CD3 epsilon/Cd3e was detected in a paraffin-embedded section of mouse lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD3 epsilon/Cd3e Antibody (A02765-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-1-cd3e-primary-antibodies-ihc-testing-3_1.jpgAnti-CD3 epsilon/Cd3e Antibody Picoband® IHC analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). <br>
CD3 epsilon/Cd3e was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD3 epsilon/Cd3e Antibody (A02765-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-1-cd3e-primary-antibodies-ihc-testing-4.jpgAnti-CD3 epsilon/Cd3e Antibody Picoband® IHC analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). <br>
CD3 epsilon/Cd3e was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD3 epsilon/Cd3e Antibody (A02765-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd5-picoband-trade-antibody-a00480-3-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-3-cd5-primary-antibodies-wb-testing-1_1.jpgAnti-CD5 Antibody Picoband® Western blot analysis of CD5 using anti-CD5 antibody (A00480-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates,<br>
Lane 2: rat thymus tissue lysates,<br>
Lane 3: mouse spleen tissue lysates,<br>
Lane 4: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody (Catalog # A00480-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD5 at approximately 70 kDa. The expected band size for CD5 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-3-cd5-primary-antibodies-ihc-testing-2_1.jpgAnti-CD5 Antibody Picoband® IHC analysis of CD5 using anti-CD5 antibody (A00480-3). <br>
CD5 was detected in a paraffin-embedded section of mouse lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD5 Antibody (A00480-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-3-cd5-primary-antibodies-ihc-testing-3_1.jpgAnti-CD5 Antibody Picoband® IHC analysis of CD5 using anti-CD5 antibody (A00480-3). <br>
CD5 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD5 Antibody (A00480-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-3-cd5-primary-antibodies-ihc-testing-4.jpgAnti-CD5 Antibody Picoband® IHC analysis of CD5 using anti-CD5 antibody (A00480-3). <br>
CD5 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD5 Antibody (A00480-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00480-3-cd5-primary-antibodies-ihc-testing-5.jpgAnti-CD5 Antibody Picoband® IHC analysis of CD5 using anti-CD5 antibody (A00480-3). <br>
CD5 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD5 Antibody (A00480-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-2b4-cd244-picoband-trade-antibody-a02527-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02527-1-cd244-primary-antibodies-wb-testing-1_1.jpgAnti-2B4/Cd244 Antibody Picoband®Western blot analysis of 2B4/Cd244 using anti-2B4/Cd244 antibody (A02527-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse spleen tissue lysates, <br>
Lane 4: mouse lung tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-2B4/Cd244 antigen affinity purified polyclonal antibody (A02527-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for 2B4/Cd244 at approximately 65-75 kDa. The expected band size for 2B4/Cd244 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02527-1-cd244-primary-antibodies-ihc-testing-1.jpgAnti-2B4/Cd244 Antibody Picoband®IHC analysis of 2B4/Cd244 using anti-2B4/Cd244 antibody (A02527-1). <br>
2B4/Cd244 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-2B4/Cd244 Antibody (A02527-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-2b4-cd244-picoband-trade-antibody-a02527-2-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02527-2-cd244-primary-antibodies-wb-testing-1.jpgAnti-2B4/Cd244 Antibody Picoband® Western blot analysis of Cd244 using anti-Cd244 antibody (A02527-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates, <br> Lane 2: rat spleen tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd244 antigen affinity purified polyclonal antibody (Catalog # A02527-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd244 at approximately 50KD. The expected band size for Cd244 is at 42KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ceacam5-picoband-trade-antibody-a00356-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-wb-testing-1.jpgAnti-CEACAM5 Antibody Picoband® Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human SW620 whole cell lysates, <br> Lane 2: human Caco-2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-2.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-3.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-4.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-5.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-6.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-7.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-8.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-9.jpgAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-ceacam5-primary-antibodies-ihc-testing-10.pngAnti-CEACAM5 Antibody Picoband® IHC analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). <br> CEACAM5 was detected in paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-CEACAM5 Antibody (A00356) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cpt2-cpt1-picoband-trade-antibody-a02112-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02112-1-cpt2-primary-antibodies-wb-testing-1_1.jpgAnti-CPT2/CPT1 Antibody Picoband®Western blot analysis of CPT2/CPT1 using anti-CPT2/CPT1 antibody (A02112-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: human HEL whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: human SiHa whole cell lysates,<br>
Lane 5: rat small intestine tissue lysates,<br>
Lane 6: rat kidney tissue lysates,<br>
Lane 7: mouse small intestine tissue lysates,<br>
Lane 8: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT2/CPT1 antigen affinity purified polyclonal antibody (A02112-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CPT2/CPT1 at approximately 74 kDa. The expected band size for CPT2/CPT1 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02112-1-cpt2-primary-antibodies-ihc-testing-1.jpgAnti-CPT2/CPT1 Antibody Picoband®IHC analysis of CPT2/CPT1 using anti-CPT2/CPT1 antibody (A02112-1). <br>CPT2/CPT1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPT2/CPT1 Antibody (A02112-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02112-1-cpt2-primary-antibodies-fcm-testing-1.jpgAnti-CPT2/CPT1 Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-CPT2/CPT1 antibody (A02112-1). <br>
Overlay histogram showing SiHa cells stained with A02112-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CPT2/CPT1 Antibody (A02112-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ddx1-picoband-trade-antibody-a03727-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-wb-testing-1.jpgAnti-DDX1 Antibody Picoband®Western blot analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human CACO-2 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human RT4 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX1 antigen affinity purified polyclonal antibody (Catalog # A03727-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX1 at approximately 88-90 kDa. The expected band size for DDX1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-2.jpgAnti-DDX1 Antibody Picoband® IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br> DDX1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-7.jpgAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>DDX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-3_1.jpgAnti-DDX1 Antibody Picoband® IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br> DDX1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-4_1.jpgAnti-DDX1 Antibody Picoband® IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br> DDX1 was detected in paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-5_1.jpgAnti-DDX1 Antibody Picoband® IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br> DDX1 was detected in paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-5.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-6.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-7.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-8.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-9.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-10.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-11.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ihc-testing-12.pngAnti-DDX1 Antibody Picoband®IHC analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-DDX1 Antibody (A03727-1) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-if-testing-6.jpgAnti-DDX1 Antibody Picoband® IF analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br> DDX1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-if-testing-2.pngAnti-DDX1 Antibody Picoband®IF analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-if-testing-3.pngAnti-DDX1 Antibody Picoband®IF analysis of DDX1 using anti-DDX1 antibody (A03727-1). <br>
DDX1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (A03727-1) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-ip-testing-1.jpgAnti-DDX1 Antibody Picoband®Immunoprecipitating (IP) DDX1 in SH-SY5Y whole cell lysate.<br>
Western blot analysis of DDX1 using anti-DDX1 antibody (A03727-1); <br>
Lane 1: SH-SY5Y whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-DDX1 antibody in SH-SY5Y whole cell lysate;<br>
Lane 3: anti-DDX1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX1 antigen affinity purified polyclonal antibody (A03727-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for DDX1 at approximately 88 kDa. The expected band size for DDX1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03727-1-ddx1-primary-antibodies-fc-testing-7.pngAnti-DDX1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-DDX1 antibody (A03727-1). <br> Overlay histogram showing SiHa cells stained with A03727-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX1 Antibody (A03727-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ddx4-mvh-antibody-picoband-a02448-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-wb-testing-1_1.jpgAnti-DDX4/MVH Antibody Picoband® Western blot analysis of DDX4 using anti-DDX4 antibody (A02448). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates,<br>
Lane 2: mouse testis tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX4 antigen affinity purified polyclonal antibody (Catalog # A02448) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX4 at approximately 79 kDa. The expected band size for DDX4 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-ihc-testing-4.jpgAnti-DDX4/MVH Antibody Picoband®IHC analysis of DDX4 using anti-DDX4 antibody (A02448). <br>DDX4 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-ihc-testing-2.jpgAnti-DDX4/MVH Antibody Picoband® IHC analysis of DDX4 using anti-DDX4 antibody (A02448). <br> DDX4 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-ihc-testing-3.jpgAnti-DDX4/MVH Antibody Picoband® IHC analysis of DDX4 using anti-DDX4 antibody (A02448). <br> DDX4 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-fc-testing-4.pngAnti-DDX4/MVH Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-DDX4 antibody (A02448). <br> Overlay histogram showing PC-3 cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-fc-testing-5.pngAnti-DDX4/MVH Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-DDX4 antibody (A02448). <br> Overlay histogram showing SiHa cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-if-testing-6.jpgAnti-DDX4/MVH Antibody Picoband® IF analysis of DDX4 using anti-DDX4 antibody (A02448). <br>
DDX4 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-if-testing-7.jpgAnti-DDX4/MVH Antibody Picoband® IF analysis of DDX4 using anti-DDX4 antibody (A02448). <br>
DDX4 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02448-ddx4-primary-antibodies-if-testing-8.jpgAnti-DDX4/MVH Antibody Picoband® IF analysis of DDX4 using anti-DDX4 antibody (A02448). <br>
DDX4 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX4 Antibody (A02448) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-dhx15-prp43-picoband-trade-antibody-a08140-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-wb-testing-1.jpgAnti-DHX15/prp43 Antibody Picoband® Western blot analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human 293T whole cell lysates, <br>
Lane 3: human CACO-2 whole cell lysates, <br>
Lane 4: human HEL whole cell lysates, <br>
Lane 5: human RT4 whole cell lysates, <br>
Lane 6: human A549 whole cell lysates, <br>
Lane 7: human A431 whole cell lysates, <br>
Lane 8: human U251 whole cell lysates, <br>
Lane 9: rat C6 whole cell lysates, <br>
Lane 10: mouse thymus tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX15/prp43 antigen affinity purified polyclonal antibody (Catalog # A08140-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX15/prp43 at approximately 91 kDa. The expected band size for DHX15/prp43 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-ihc-testing-2_1.jpgAnti-DHX15/prp43 Antibody Picoband® IHC analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). <br>
DHX15/prp43 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX15/prp43 Antibody (A08140-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-ihc-testing-3_1.jpgAnti-DHX15/prp43 Antibody Picoband® IHC analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). <br>
DHX15/prp43 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX15/prp43 Antibody (A08140-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-ihc-testing-4_1.jpgAnti-DHX15/prp43 Antibody Picoband® IHC analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). <br>
DHX15/prp43 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX15/prp43 Antibody (A08140-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-ihc-testing-5_1.jpgAnti-DHX15/prp43 Antibody Picoband® IHC analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1). <br>
DHX15/prp43 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX15/prp43 Antibody (A08140-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-if-testing-6.jpgAnti-DHX15/prp43 Antibody Picoband® IF analysis of DHX15/prp43 using anti-DHX15/prp43 antibody (A08140-1) and anti-Beta Tubulin antibody (M01857-3).<br>
DHX15/prp43 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX15/prp43 Antibody (A08140-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08140-1-dhx15-primary-antibodies-fcm-testing-7.jpgAnti-DHX15/prp43 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-DHX15/prp43 antibody (A08140-1). <br>
Overlay histogram showing Hela cells stained with A08140-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX15/prp43 Antibody (A08140-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-desmoglein-2-dsg2-picoband-trade-antibody-a02035-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-wb-testing-1_1.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® Western blot analysis of DSG2 using anti-DSG2 antibody (A02035). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSG2 antigen affinity purified polyclonal antibody (Catalog # A02035) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DSG2 at approximately 160 kDa. The expected band size for DSG2 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-ihc-testing-2.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® IHC analysis of DSG2 using anti-DSG2 antibody (A02035). <br> DSG2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-ihc-testing-3.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® IHC analysis of DSG2 using anti-DSG2 antibody (A02035). <br> DSG2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-ihc-testing-4.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® IHC analysis of DSG2 using anti-DSG2 antibody (A02035). <br> DSG2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-fc-testing-5.pngAnti-Desmoglein 2/DSG2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-DSG2 antibody (A02035). <br> Overlay histogram showing RH35 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-fc-testing-6.pngAnti-Desmoglein 2/DSG2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-DSG2 antibody (A02035). <br> Overlay histogram showing U87 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-fc-testing-7.pngAnti-Desmoglein 2/DSG2 Antibody Picoband® Flow Cytometry analysis of HEPA 1-6 cells using anti-DSG2 antibody (A02035). <br> Overlay histogram showing HEPA 1-6 cells stained with A02035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (A02035, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02035-dsg2-primary-antibodies-if-testing-8.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® IF analysis of DSG2 using anti-DSG2 antibody (A02035). <br>
DSG2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DSG2 Antibody (A02035) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-fen1-picoband-trade-antibody-a01484-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-wb-testing-1.jpgAnti-FEN1 Antibody Picoband® Western blot analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Daudi whole cell lysates,<br>
Lane 3: human U20S whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse SP2/0 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FEN1 antigen affinity purified polyclonal antibody (Catalog # A01484-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FEN1 at approximately 43 kDa. The expected band size for FEN1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-ihc-testing-2.jpgAnti-FEN1 Antibody Picoband® IHC analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-ihc-testing-3.jpgAnti-FEN1 Antibody Picoband® IHC analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-ihc-testing-4.jpgAnti-FEN1 Antibody Picoband® IHC analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-ihc-testing-5.jpgAnti-FEN1 Antibody Picoband® IHC analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-ihc-testing-6.jpgAnti-FEN1 Antibody Picoband® IHC analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-if-testing-7.jpgAnti-FEN1 Antibody Picoband® IF analysis of FEN1 using anti-FEN1 antibody (A01484-1) and anti-Beta Tubulin antibody (M01857-3).<br>
FEN1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FEN1 Antibody (A01484-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-if-testing-8.jpgAnti-FEN1 Antibody Picoband® IF analysis of FEN1 using anti-FEN1 antibody (A01484-1). <br>
FEN1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FEN1 Antibody (A01484-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01484-1-fen1-primary-antibodies-fcm-testing-9.jpgAnti-FEN1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-FEN1 antibody (A01484-1). <br>
Overlay histogram showing K562 cells stained with A01484-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FEN1 Antibody (A01484-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-fhl-picoband-trade-antibody-a01258-1-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-1-fhl-primary-antibodies-wb-testing-1_1.jpgAnti-FHL FHL1 Antibody Picoband® Western blot analysis of FHL using anti-FHL antibody (A01258-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-1-fhl-primary-antibodies-ihc-testing-2_1.jpgAnti-FHL FHL1 Antibody Picoband® IHC analysis of FHL using anti-FHL antibody (A01258-1). <br> FHL was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FHL Antibody (A01258-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-1-fhl-primary-antibodies-ihc-testing-3_1.jpgAnti-FHL FHL1 Antibody Picoband® IHC analysis of FHL using anti-FHL antibody (A01258-1). <br> FHL was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FHL Antibody (A01258-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-1-fhl-primary-antibodies-if-testing-4_1.jpgAnti-FHL FHL1 Antibody Picoband® IF analysis of FHL using anti-FHL antibody (A01258-1). <br> FHL was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FHL Antibody (A01258-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-1-fhl-primary-antibodies-fc-testing-5_1.pngAnti-FHL FHL1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-FHL antibody (A01258-1). <br> Overlay histogram showing PC-3 cells stained with A01258-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FHL Antibody (A01258-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fhl-picoband-trade-antibody-a01258-2-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-2-fhl-primary-antibodies-wb-testing-1_1.jpgAnti-FHL Fhl1 Antibody Picoband® Western blot analysis of FHL using anti-FHL antibody (A01258-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: mouse skeletal muscle tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01258-2-fhl-primary-antibodies-fc-testing-2_1.pngAnti-FHL Fhl1 Antibody Picoband® Flow Cytometry analysis of Ana-1 cells using anti-FHL antibody (A01258-2). <br> Overlay histogram showing Ana-1 cells stained with A01258-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FHL Antibody (A01258-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fascin-2-fscn2-picoband-trade-antibody-a07840-2-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07840-2-fscn2-primary-antibodies-wb-testing-1_1.jpgAnti-Fascin 2/FSCN2 Antibody Picoband® Western blot analysis of FSCN2 using anti-FSCN2 antibody (A07840-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human U-87MG whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: rat spleen tissue lysates, <br> Lane 5: rat ovary tissue lysates, <br> Lane 6: mouse lung tissue lysates, <br> Lane 7: mouse ovary tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN2 antigen affinity purified polyclonal antibody (Catalog # A07840-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSCN2 at approximately 60KD. The expected band size for FSCN2 is at 55KD.
https://www.bosterbio.com/products/primary-antibodies/anti-glb1-beta-galactosidase-antibody-picoband-a01829-2-boster.html2026-03-24T05:25:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-ijms-26-02564-g003.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband®RPSA and GLB1 proteomic expression from CPTAC dataset. The proteomic expression levels of RPSA and GLB1 (β-galactosidase) were analyzed in 56 colorectal tumors (in red) and their matched normal mucosa samples (in blue) from the CPTAC dataset (Series #3, ) and expressed as the logarithmic difference in protein abundance, measured by mass spectrometry. ( A ) Unshared log 2 ratio expression of RPSA (upper panel) and GLB1 (lower panel). ( B ) Expression of RPSA (upper panels) and GLB1 (lower panels) based on transcriptomic subtypes associated with CMS1 (microsatellite instability and immune infiltration), CMS2 (WNT and MYC signaling activation), CMS3 (KRAS mutation and metabolic deregulation), CMS4 (TGFβ activation in EMT and ECM remodeling). Data are expressed as the mean ± SEM. Statistical test: paired Wilcoxon test (ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11942345/'>40141206</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-ijms-26-02564-g004.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband®Expression of RPSA, GLB1, and GLB1 variant 67EBP transcripts in CRC tissues. Expression levels of RPSA , GLB1, and GLB1 variant 67EBP were evaluated at the transcript levels in 25 CRC samples and corresponding resection margins from the biobank collection (Series #2, ) using quantitative RT-PCR. ( A ) Expression of RPSA . ( B ) Expression of GLB1 using primer set #1 targeting both β-galactosidase and 67EBP mRNA. ( C ) Expression of GLB1 using primer set #2 targeting exons 2–5 specific to the mRNA splice variant of 67EBP . ( D ) Expression of GLB1 using primer set #3 targeting exons 5–7 specific to the mRNA splice variant of 67EBP . RPLP0 was used as the normalizer. ( A – D , left): Expression levels were quantified using the Pfaffl method and compared to the resection margin. Results are presented as mean ± SEM. Statistical test: paired Wilcoxon test; (ns, not significant; #, p = 0.07; **, p < 0.01; ***, p < 0.001; ( A – D , right): Graphs displaying the relative amounts of RPSA and 67EBP in individual tumor tissue (T) compared to the resection margin (RM). Red lines are for a ratio of 1.0. Sample size: n = 25.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11942345/'>40141206</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-ijms-26-02564-g005.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband®Expression of RPSA and GLB1 transcripts using a GEO DNA microarray dataset from patients with colorectal cancer. Analysis of RPSA and GLB1 transcript levels in primary tumors and their resection margins from the GEO microarray dataset (Series #4, ) using the Affymetrix Human Genome U133A Array. Only pairs whose expression was available in both the primary tumor and the corresponding resection margin were used. ( A ) RPSA expression analyzed using probe 213801_x_at. ( B ) GLB1 expression analyzed using probe 201576_s_at. Results are presented as mean ± SEM. Statistical significance was determined using the paired Wilcoxon test (ns, non-significant; ***, p < 0.001). Red lines are for a ratio of 1.0. Sample size: n = 44.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11942345/'>40141206</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-glb1-primary-antibodies-fcm-testing-5.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-GLB1/Beta-galactosidase antibody (A01829-2). <br>Overlay histogram showing MCF-7 cells stained with A01829-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLB1/Beta-galactosidase Antibody (A01829-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-glb1-primary-antibodies-wb-testing-1_1.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® Western blot analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLB1/Beta-galactosidase antigen affinity purified polyclonal antibody (Catalog # A01829-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLB1/Beta-galactosidase at approximately 65-85 kDa. The expected band size for GLB1/Beta-galactosidase is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-glb1-primary-antibodies-ihc-testing-2_1.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® IHC analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). <br>
GLB1/Beta-galactosidase was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLB1/Beta-galactosidase Antibody (A01829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-glb1-primary-antibodies-ihc-testing-3_1.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® IHC analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). <br>
GLB1/Beta-galactosidase was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLB1/Beta-galactosidase Antibody (A01829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-2-glb1-primary-antibodies-if-testing-4.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® IF analysis of GLB1/Beta-galactosidase using anti-GLB1/Beta-galactosidase antibody (A01829-2). <br>
GLB1/Beta-galactosidase was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GLB1/Beta-galactosidase Antibody (A01829-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-glb1-beta-galactosidase-antibody-picoband-a01829-3-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-3-glb1-primary-antibodies-wb-testing-1_2.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® Western blot analysis of GLB1 using anti-GLB1 antibody (A01829-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse liver tissue lysates, <br> Lane 5: mouse testis tissue lysates, <br> Lane 6: mouse HEPA1-6 whole cell lysates, <br> Lane 7: mouse Neuro-2a whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLB1 antigen affinity purified polyclonal antibody (Catalog # A01829-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for GLB1 at approximately 65, 76, 85KD. The expected band size for GLB1 is at 76KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-3-glb1-primary-antibodies-ihc-testing-2_2.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® IHC analysis of GLB1 using anti-GLB1 antibody (A01829-3). <br> GLB1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLB1 Antibody (A01829-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-3-glb1-primary-antibodies-fc-testing-3_2.pngAnti-GLB1/Beta-galactosidase Antibody Picoband® Flow Cytometry analysis of HEPA 1-6 cells using anti-GLB1 antibody (A01829-3). <br> Overlay histogram showing HEPA 1-6 cells stained with A01829-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLB1 Antibody (A01829-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-3-glb1-primary-antibodies-fc-testing-4_2.pngAnti-GLB1/Beta-galactosidase Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-GLB1 antibody (A01829-3). <br> Overlay histogram showing RH35 cells stained with A01829-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLB1 Antibody (A01829-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01829-3-glb1-primary-antibodies-if-testing-5.jpgAnti-GLB1/Beta-galactosidase Antibody Picoband® IF analysis of GLB1 using anti-GLB1 antibody (A01829-3). <br>
GLB1 was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GLB1 Antibody (A01829-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-hif3-hif3a-picoband-trade-antibody-a03805-3-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03805-3-hif3a-primary-antibodies-wb-testing-1_1.jpgAnti-HIF3/HIF3A Antibody Picoband® Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human U-87MG whole cell lysates, <br> Lane 5: human U2OS whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> Lane 7: human PC-3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03805-3-hif3a-primary-antibodies-wb-testing-2_1.jpgAnti-HIF3/HIF3A Antibody Picoband® Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat skeletal muscle tissue lysates, <br> Lane 3: mouse lung tissue lysates, <br> Lane 4: mouse NIH3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03805-3-hif3a-primary-antibodies-fc-testing-3_1.pngAnti-HIF3/HIF3A Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-HIF3A antibody (A03805-3). <br> Overlay histogram showing U87 cells stained with A03805-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF3A Antibody (A03805-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-histone-h3-picoband-trade-antibody-a12477-2-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-12864_2024_10120_fig1_html.pngAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®Expression levels of EZH2, JMJD3, H3K27me3 in spermatogonia. ( A ) qRT-PCR was used to detect the expression of EZH2 siRNA and JMJD3 siRNA in spermatogonia after interference. ( B ) measurements of EZH2 and JMJD3 mRNA levels in spermatogonia after overexpression. ( C ) The protein levels of EZH2, JMJD3 and H3K27me3 in spermatogonia were detected and statistically analyzed by Western blot. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-12864_2024_10120_fig4_html.pngAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®TET1 coordinates with H3K27me3 to target Pramel3 to promote its activation and expression. ( A ) qRT-PCR was used to detect the expression of genes enriched by Chip-seq after JMJD3 overexpression. ( B ) Peak plots of 2610002M06Rik and Pramel3 target genes obtained from TET1 overexpressing cells deposited with H3K27me3 antibody <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-12864_2024_10120_fig5_html.pngAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®TET1-H3K27me3 regulated through PI3K-AKT pathway. ( A ) KEGG enrichment analysis. ( B ) Western blot was used to detect the protein expressions of AKT and P-AKT in the PI3K-AKT pathway after JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-12864_2024_10120_fig6_html.pngAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®In vivo functional validation of JMJD3. ( A ) Spermatogenesis disorder model mice transplantation of control PCDH and JMJD3 Positive Cells. ( B ) Chart of comparison of control PCDH with testes transplanted with JMJD3 positive cells. ( C ) HE staining plot of JMJD3 overexpression versus control PCDH. ( D ) Expression of JMJD3 in testicular spermatogonia after overexpression of JMJD3. ( E ) Expression of H3K27me3 and PCNA in testicular spermatogonia after JMJD3 overexpression. ( F ) Statistical analysis of JMJD3 + cells/H3K27m3 + cells and PCNA + cells in immunohistochemistry. Scale bar = 50 μm. n = 3 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-wb-testing-1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human 22RV1 whole cell lysates, <br>
Lane 3: human CACO-2 whole cell lysates, <br>
Lane 4: human CCRF-CEM whole cell lysates, <br>
Lane 5: human Hela whole cell lysates, <br>
Lane 6: human HepG2 whole cell lysates, <br>
Lane 7: human THP-1 whole cell lysates, <br>
Lane 8: human U2OS whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 17 kDa. The expected band size for Histone H3 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-wb-testing-2.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® Western blot analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: rat PC-12 whole cell lysates, <br>
Lane 4: rat C6 whole cell lysates, <br>
Lane 5: mouse brain tissue lysates, <br>
Lane 6: mouse liver tissue lysates, <br>
Lane 7: mouse HEPA1-6 whole cell lysates, <br>
Lane 8: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (Catalog # A12477-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 17 kDa. The expected band size for Histone H3 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-3_1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-4_1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-5_1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-6.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-7_1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-8_1.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-9.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-10.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-11.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-12.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-13.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-14.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-15.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-16.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-17.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-18.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-19.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-20.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-21.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-ihc-testing-22.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (A12477-2). <br>
Histone H3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (A12477-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-if-testing-23.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® IF analysis of Histone H3 using anti-Histone H3 antibody (A12477-2) and anti-Beta Tubulin antibody (M01857-3).<br>
Histone H3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Histone H3 Antibody (A12477-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-fcm-testing-24.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Histone H3 antibody (A12477-2). <br>
Overlay histogram showing RAW264.7 cells stained with A12477-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H3 Antibody (A12477-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12477-2-histone-h3-primary-antibodies-fcm-testing-25.jpgAnti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Histone H3 antibody (A12477-2). <br>
Overlay histogram showing C6 cells stained with A12477-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H3 Antibody (A12477-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-d-auf1-hnrnpd-picoband-trade-antibody-a09982-1-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-wb-testing-1.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human HL-60 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human HepG2 whole cell lysates, <br>
Lane 7: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd_-primary-antibodies-ihc-testing-10.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).
HNRNPD was detected in paraffin-embedded section of mouse intestine tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-wb-testing-2.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® Western blot analysis of HNRNPD using anti-HNRNPD antibody (A09982-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: mouse brain tissue lysates, <br>
Lane 4: mouse thymus tissue lysates, <br>
Lane 5: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (Catalog # A09982-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for HNRNPD at approximately 37-45KD. The expected band sizes for HNRNPD are at 37, 40, 42 and 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-ihc-testing-3.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in paraffin-embedded section of B lymphocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-ihc-testing-4.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-ihc-testing-5.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-ihc-testing-6.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C.Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-fcm-testing-7.pngAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-HNRNPD antibody (A09982-1). <br>Overlay histogram showing A431 cells stained with A09982-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPD Antibody (A09982-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-if-testing-8.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IF analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09982-1-hnrnpd-primary-antibodies-if-testing-9.jpgAnti-hnRNP D/AUF1/HNRNPD Antibody Picoband® IF analysis of HNRNPD using anti-HNRNPD antibody (A09982-1). <br>
HNRNPD was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HNRNPD Antibody (A09982-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-hsd3b1-antibody-picoband-a02856-2-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02856-2-hsd3b1-primary-antibodies-wb-testing-1_1.jpgAnti-HSD3B1 Antibody Picoband® Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human Caco-2 whole cell lysates, <br> Lane 3: human MDA-MB-453 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B1 antigen affinity purified polyclonal antibody (Catalog # A02856-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42KD. The expected band size for HSD3B1 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02856-2-hsd3b1-primary-antibodies-ihc-testing-2_1.jpgAnti-HSD3B1 Antibody Picoband® IHC analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). <br> HSD3B1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD3B1 Antibody (A02856-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02856-2-hsd3b1-primary-antibodies-fc-testing-3_1.pngAnti-HSD3B1 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-HSD3B1 antibody (A02856-2). <br> Overlay histogram showing CACO-2 cells stained with A02856-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD3B1 Antibody (A02856-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02856-2-hsd3b1-primary-antibodies-if-testing-4.jpgAnti-HSD3B1 Antibody Picoband® IF analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). <br>
HSD3B1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSD3B1 Antibody (A02856-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-il18-picoband-trade-antibody-a00124-1-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-1-il18-primary-antibodies-wb-testing-1.jpgAnti-IL18 Antibody Picoband® Western blot analysis of IL18 using anti-IL18 antibody (A00124-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates,<br>
Lane 2: rat spleen tissue lysates,<br>
Lane 3: rat lung tissue lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: mouse thymus tissue lysates,<br>
Lane 6: mouse spleen tissue lysates,<br>
Lane 7: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # A00124-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-1-il18-primary-antibodies-fcm-testing-2.jpgAnti-IL18 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-IL18 antibody (A00124-1). <br>
Overlay histogram showing ANA-1 cells stained with A00124-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (A00124-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00124-1-il18-primary-antibodies-fcm-testing-3.jpgAnti-IL18 Antibody Picoband® Flow Cytometry analysis of mouse spleen tissues using anti-IL18 antibody (A00124-1). <br>
Overlay histogram showing mouse spleen tissues stained with A00124-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (A00124-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-acetyl-coenzyme-a-carboxylase-acabc-picoband-trade-antibody-a02828-2-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-wb-testing-1_1_1.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® Western blot analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human RT4 whole cell lysates,<br>
Lane 2: human Caco-2 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 20/Krt20 antigen affinity purified polyclonal antibody (Catalog # A02828-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 20/Krt20 at approximately 48 kDa. The expected band size for Cytokeratin 20/Krt20 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-2_1.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2). <br> Krt20 was detected in paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Krt20 Antibody (A02828-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-3_1.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2). <br> Krt20 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Krt20 Antibody (A02828-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-4_1.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2). <br> Krt20 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Krt20 Antibody (A02828-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-5.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-6.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-7.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-8.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-ihc-testing-9.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IHC analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-if-testing-10.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® IF analysis of Krt20 using anti-Krt20 antibody (A02828-2). <br>
Krt20 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Krt20 Antibody (A02828-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-if-testing-11.jpgAnti-Cytokeratin 20/Krt20 Antibody Picoband® IF analysis of Krt20 using anti-Krt20 antibody (A02828-2). <br>
Krt20 was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Krt20 Antibody (A02828-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-if-testing-12.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IF analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-if-testing-13.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IF analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-if-testing-14.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® IF analysis of Cytokeratin 20/Krt20 using anti-Cytokeratin 20/Krt20 antibody (A02828-2). <br>
Cytokeratin 20/Krt20 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 20/Krt20 Antibody (A02828-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-fcm-testing-15.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2). <br> Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-fcm-testing-16.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Krt20 antibody (A02828-2). <br> Overlay histogram showing Hela cells stained with A02828-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02828-2-krt20-primary-antibodies-fcm-testing-17.pngAnti-Cytokeratin 20/Krt20 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-Krt20 antibody (A02828-2). <br> Overlay histogram showing RH35 cells stained with A02828-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Krt20 Antibody (A02828-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-c-maf-maf-picoband-trade-antibody-a00654-1-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00654-1-maf-primary-antibodies-wb-testing-1.jpgAnti-c-Maf/MAF Antibody Picoband®Western blot analysis of c-Maf/MAF using anti-c-Maf/MAF antibody (A00654-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Maf/MAF antigen affinity purified polyclonal antibody (Catalog # A00654-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for c-Maf/MAF at approximately 52 kDa. The expected band size for c-Maf/MAF is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00654-1-maf-primary-antibodies-if-testing-1.jpgAnti-c-Maf/MAF Antibody Picoband®IF analysis of c-Maf/MAF using anti-c-Maf/MAF antibody (A00654-1) and anti-Tubulin Alpha antibody (M03989-3).<br>
c-Maf/MAF was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-c-Maf/MAF Antibody (A00654-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-mag-picoband-trade-antibody-a03019-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03019-mag-primary-antibodies-wb-testing-1.jpgAnti-MAG Antibody Picoband® Western blot analysis of MAG using anti-MAG antibody (A03019). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAG antigen affinity purified polyclonal antibody (Catalog # A03019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAG at approximately 100 kDa. The expected band size for MAG is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03019-mag-primary-antibodies-fc-testing-2_1.pngAnti-MAG Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-MAG antibody (A03019). <br> Overlay histogram showing PC-3 cells stained with A03019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03019-mag-primary-antibodies-fcm-testing-3.jpgAnti-MAG Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-MAG antibody (A03019). <br>
Overlay histogram showing K562 cells stained with A03019 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MAG Antibody (A03019, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nat8l-antibody-picoband-a07393-2-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07393-2-nat8l-primary-antibodies-fc-testing-2_1.pngAnti-NAT8L Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-NAT8L antibody (A07393-2). <br>Overlay histogram showing U20S cells stained with A07393-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAT8L Antibody (A07393-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07393-2-nat8l-primary-antibodies-wb-testing-1_1.jpgAnti-NAT8L Antibody Picoband® Western blot analysis of NAT8L using anti-NAT8L antibody (A07393-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT8L antigen affinity purified polyclonal antibody (Catalog # A07393-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for NAT8L at approximately 33-40KD. The expected band size for NAT8L is at 33KD.
https://www.bosterbio.com/products/primary-antibodies/anti-hec1-hec-ndc80-picoband-trade-antibody-a01731-2-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-wb-testing-1_1.jpgAnti-HEC1/HEC/NDC80 Antibody Picoband® Western blot analysis of NDC80 using anti-NDC80 antibody (A01731-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: mouse spleen tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDC80 antigen affinity purified polyclonal antibody (Catalog # A01731-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDC80 at approximately 74KD. The expected band size for NDC80 is at 74KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-if-testing-2_1.jpgAnti-HEC1/HEC/NDC80 Antibody Picoband® IF analysis of NDC80 using anti-NDC80 antibody (A01731-2). <br> NDC80 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NDC80 Antibody (A01731-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-fc-testing-3_1.pngAnti-HEC1/HEC/NDC80 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-NDC80 antibody (A01731-2). <br> Overlay histogram showing HL-60 cells stained with A01731-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDC80 Antibody (A01731-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-fc-testing-4_1.pngAnti-HEC1/HEC/NDC80 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-NDC80 antibody (A01731-2). <br> Overlay histogram showing SiHa cells stained with A01731-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDC80 Antibody (A01731-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-if-testing-5.jpgAnti-HEC1/HEC/NDC80 Antibody Picoband® IF analysis of NDC80 using anti-NDC80 antibody (A01731-2). <br>
NDC80 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NDC80 Antibody (A01731-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01731-2-ndc80-primary-antibodies-if-testing-6.jpgAnti-HEC1/HEC/NDC80 Antibody Picoband® IF analysis of NDC80 using anti-NDC80 antibody (A01731-2). <br>
NDC80 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NDC80 Antibody (A01731-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-neurofibromin-nf1-picoband-trade-antibody-a00043-1-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-wb-testing-1_1.jpgAnti-Neurofibromin/NF1 Antibody Picoband® Western blot analysis of NF1 using anti-NF1 antibody (A00043-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF1 antigen affinity purified polyclonal antibody (Catalog # A00043-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF1 at approximately 319KD. The expected band size for NF1 is at 319KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-ihc-testing-2_1.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IHC analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-ihc-testing-3_1.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IHC analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-ihc-testing-4.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IHC analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-ihc-testing-5.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IHC analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-fc-testing-8.pngAnti-Neurofibromin/NF1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-NF1 antibody (A00043-1). <br> Overlay histogram showing A549 cells stained with A00043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF1 Antibody (A00043-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-ihc-testing-6.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IHC analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in a paraffin-embedded section of human seminoma testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-fc-testing-9.pngAnti-Neurofibromin/NF1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NF1 antibody (A00043-1). <br> Overlay histogram showing Hela cells stained with A00043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF1 Antibody (A00043-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00043-1-nf1-primary-antibodies-if-testing-7.jpgAnti-Neurofibromin/NF1 Antibody Picoband® IF analysis of NF1 using anti-NF1 antibody (A00043-1). <br>
NF1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NF1 Antibody (A00043-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-nup214-picoband-trade-antibody-a02408-boster.html2026-03-24T05:25:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nf1-primary-antibodies-wb-testing-1_1.jpgAnti-NUP214 Antibody Picoband® Western blot analysis of NUP214 using anti-NUP214 antibody (A02408). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human THP-1 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP214 antigen affinity purified polyclonal antibody (Catalog # A02408) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP214 at approximately 280KD. The expected band size for NUP214 is at 214KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nup214-primary-antibodies-wb-testing-2_1.jpgAnti-NUP214 Antibody Picoband® Western blot analysis of NUP214 using anti-NUP214 antibody (A02408). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse spleen tissue lysates, <br>
Lane 2: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP214 antigen affinity purified polyclonal antibody (Catalog # A02408) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP214 at approximately 280KD. The expected band size for NUP214 is at 214KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nup214-primary-antibodies-if-testing-3.jpgAnti-NUP214 Antibody Picoband® IF analysis of NUP214 using anti-NUP214 antibody (A02408). <br>
NUP214 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NUP214 Antibody (A02408) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nup214-primary-antibodies-if-testing-4.jpgAnti-NUP214 Antibody Picoband® IF analysis of NUP214 using anti-NUP214 antibody (A02408). <br>
NUP214 was detected in immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NUP214 Antibody (A02408) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nup214-primary-antibodies-fc-testing-5.pngAnti-NUP214 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-NUP214 antibody (A02408). <br>Overlay histogram showing HL-60 cells stained with A02408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP214 Antibody (A02408, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02408-nup214-primary-antibodies-fc-testing-6.pngAnti-NUP214 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-NUP214 antibody (A02408). <br>Overlay histogram showing HEPA1-6 cells stained with A02408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP214 Antibody (A02408, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rtl10-picoband-trade-antibody-a31740-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31740-rtl10-primary-antibodies-wb-testing-1_1.jpgAnti-RTL10 Antibody Picoband® Western blot analysis of RTL10 using anti-RTL10 antibody (A31740). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human HL-60 whole cell lysates, <br> Lane 4: human U2OS whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: rat lung tissue lysates, <br> Lane 7: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL10 antigen affinity purified polyclonal antibody (Catalog # A31740) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RTL10 at approximately 36-39KD. The expected band size for RTL10 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31740-rtl10-primary-antibodies-fc-testing-2_1.pngAnti-RTL10 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RTL10 antibody (A31740). <br> Overlay histogram showing HL-60 cells stained with A31740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTL10 Antibody (A31740, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31740-rtl10-primary-antibodies-fc-testing-3_1.pngAnti-RTL10 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-RTL10 antibody (A31740). <br> Overlay histogram showing PC-3 cells stained with A31740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTL10 Antibody (A31740, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-scn11a-picoband-trade-antibody-a04126-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04126-scn11a-primary-antibodies-wb-testing-1_1.jpgAnti-SCN11A Antibody Picoband® Western blot analysis of SCN11A using anti-SCN11A antibody (A04126). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: rat kidney tissue lysates, <br> Lane 4: rat C6 whole cell lysates, <br> Lane 5: mouse spleen tissue lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse kidney tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN11A antigen affinity purified polyclonal antibody (Catalog # A04126) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SCN11A at approximately 250KD. The expected band size for SCN11A is at 205KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04126-scn11a-primary-antibodies-ihc-testing-4.jpgAnti-SCN11A Antibody Picoband®IHC analysis of SCN11A using anti-SCN11A antibody (A04126). <br>SCN11A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCN11A Antibody (A04126) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04126-scn11a-primary-antibodies-ihc-testing-2_1.jpgAnti-SCN11A Antibody Picoband® IHC analysis of SCN11A using anti-SCN11A antibody (A04126). <br> SCN11A was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SCN11A Antibody (A04126) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04126-scn11a-primary-antibodies-ihc-testing-3_1.jpgAnti-SCN11A Antibody Picoband® IHC analysis of SCN11A using anti-SCN11A antibody (A04126). <br> SCN11A was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SCN11A Antibody (A04126) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04126-scn11a-primary-antibodies-fc-testing-4_1.pngAnti-SCN11A Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SCN11A antibody (A04126). <br> Overlay histogram showing U87 cells stained with A04126 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN11A Antibody (A04126, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sfrp2-antibody-picoband-a01752-1-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01752-1-sfrp2-primary-antibodies-wb-testing-1_1.jpgAnti-SFRP2 Antibody Picoband® Western blot analysis of SFRP2 using anti-SFRP2 antibody (A01752-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human U-87MG whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP2 antigen affinity purified polyclonal antibody (Catalog # A01752-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP2 at approximately 38KD. The expected band size for SFRP2 is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01752-1-sfrp2-primary-antibodies-if-testing-2.jpgAnti-SFRP2 Antibody Picoband® IF analysis of SFRP2 using anti-SFRP2 antibody (A01752-1). <br>
SFRP2 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SFRP2 Antibody (A01752-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01752-1-sfrp2-primary-antibodies-fc-testing-3_1.pngAnti-SFRP2 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-SFRP2 antibody (A01752-1). <br> Overlay histogram showing CACO-2 cells stained with A01752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP2 Antibody (A01752-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01752-1-sfrp2-primary-antibodies-fc-testing-4_1.pngAnti-SFRP2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SFRP2 antibody (A01752-1). <br> Overlay histogram showing Hela cells stained with A01752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP2 Antibody (A01752-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ska2-picoband-trade-antibody-a06326-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06326-ska2-primary-antibodies-wb-testing-1_1.jpgAnti-SKA2 Antibody Picoband® Western blot analysis of SKA2 using anti-SKA2 antibody (A06326). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human U2OS whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human HEK293 whole cell lysates, <br> Lane 5: human HL-60 whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> Lane 7: human Caco-2 whole cell lysates, <br> Lane 8: human Hela whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKA2 antigen affinity purified polyclonal antibody (Catalog # A06326) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKA2 at approximately 20KD. The expected band size for SKA2 is at 14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06326-ska2-primary-antibodies-fc-testing-2_1.pngAnti-SKA2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SKA2 antibody (A06326). <br> Overlay histogram showing 293T cells stained with A06326 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKA2 Antibody (A06326, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06326-ska2-primary-antibodies-fc-testing-3_1.pngAnti-SKA2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SKA2 antibody (A06326). <br> Overlay histogram showing U20S cells stained with A06326 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKA2 Antibody (A06326, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-smc4-picoband-trade-antibody-a04887-1-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04887-1-smc4-primary-antibodies-wb-testing-1.jpgAnti-SMC4 Antibody Picoband®Western blot analysis of SMC4 using anti-SMC4 antibody (A04887-1). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human PC-3 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC4 antigen affinity purified polyclonal antibody (A04887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMC4 at approximately 160-170 kDa. The expected band size for SMC4 is at 147 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04887-1-smc4-primary-antibodies-if-testing-1.jpgAnti-SMC4 Antibody Picoband®IF analysis of SMC4 using anti-SMC4 antibody (A04887-1) and anti-Tubulin Alpha antibody (M03989-3).<br>
SMC4 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC4 Antibody (A04887-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04887-1-smc4-primary-antibodies-fcm-testing-1.jpgAnti-SMC4 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SMC4 antibody (A04887-1). <br>
Overlay histogram showing PC-3 cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC4 Antibody (A04887-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-socs5-picoband-trade-antibody-a05989-1-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05989-1-socs5-primary-antibodies-wb-testing-1.jpgAnti-SOCS5 Antibody Picoband®Western blot analysis of SOCS5 using anti-SOCS5 antibody (A05989-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U87 whole cell lysates,<br>
Lane 2: human U2OS whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: human 293T whole cell lysates,<br>
Lane 6: human Hela whole cell lysates,<br>
Lane 7: rat brain tissue lysates,<br>
Lane 8: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS5 antigen affinity purified polyclonal antibody (A05989-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOCS5 at approximately 61 kDa. The expected band size for SOCS5 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05989-1-socs5-primary-antibodies-ihc-testing-1.jpgAnti-SOCS5 Antibody Picoband®IHC analysis of SOCS5 using anti-SOCS5 antibody (A05989-1). <br>SOCS5 was detected in a paraffin-embedded section of human ovarican cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS5 Antibody (A05989-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05989-1-socs5-primary-antibodies-ihc-testing-2.jpgAnti-SOCS5 Antibody Picoband®IHC analysis of SOCS5 using anti-SOCS5 antibody (A05989-1). <br>SOCS5 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS5 Antibody (A05989-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-traf6-picoband-trade-antibody-a00185-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-traf6-primary-antibodies-wb-testing-1_1.jpgAnti-TRAF6 Antibody Picoband® Western blot analysis of TRAF6 using anti-TRAF6 antibody (A00185). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat RH35 whole cell lysates, <br> Lane 2: mouse thymus tissue lysates, <br> Lane 3: mouse spleen tissue lysates, <br> Lane 4: mouse RAW246.7 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6 antigen affinity purified polyclonal antibody (Catalog # A00185) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6 at approximately 60-65KD. The expected band size for TRAF6 is at 60KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-medscimonit-27-e929438-g002.jpgAnti-TRAF6 Antibody Picoband®Lipopolysaccharide (LPS)-mediated Toll-like receptor 4 (TLR4)-related nuclear factor kappa b (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were inhibited by Zi Qi decoction in vitro. ( A ) The effects of Zi Qi decoction on the relative messenger RNA level of cellular TLR4, MyD88, and TRAF6 in each group. ( B ) The effects of Zi Qi decoction on the protein level of cellular TLR4, MyD88, TRAF6, and phospho-NF-κB p65 in each group. ( C ) Zi Qi decoction significantly suppressed the nuclear transportation of activated NF-κB p65 in LPS-induced LX-2 cells, as determined by immunofluorescence (n=3). Magnification ×40. ( D ) The protein expressions of ERK, c-Jun NH2-terminal kinase, and p38 and their relative phosphorylation levels in each group. # P <0.05, compared with the control group. * P <0.05, ** P <0.01, compared with the LPS group. Data were presented as means±standard deviations.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8054620/&orig_host=www.ncbi.nlm.nih.gov'>33850093</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-medscimonit-27-e929438-g005.jpgAnti-TRAF6 Antibody Picoband®Toll-like receptor 4 (TLR4)-related nuclear factor kappa b (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were inhibited by Zi Qi decoction in vivo. ( A ) Messenger RNA expression of TLR4, MyD88, and TRAF6 in liver tissue in each group. ( B ) Protein expression of TLR4, MyD88, TRAF6, and phospho-NF-κB p65 in liver tissue in each group. ( C ) Expression of MAPK pathway-related proteins and their relative phosphorylation levels in liver tissue in each group. # P <0.05, compared with the control group. * P <0.05, *** P <0.001, compared with the vehicle group. Data were presented as means±standard deviations.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8054620/&orig_host=www.ncbi.nlm.nih.gov'>33850093</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-traf6-primary-antibodies-ihc-testing-2_1.jpgAnti-TRAF6 Antibody Picoband® IHC analysis of TRAF6 using anti-TRAF6 antibody (A00185). <br> TRAF6 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAF6 Antibody (A00185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-traf6-primary-antibodies-ihc-testing-3_1.jpgAnti-TRAF6 Antibody Picoband® IHC analysis of TRAF6 using anti-TRAF6 antibody (A00185). <br> TRAF6 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAF6 Antibody (A00185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00185-traf6-primary-antibodies-fc-testing-4_1.pngAnti-TRAF6 Antibody Picoband® Flow Cytometry analysis of HEPA 1-6 cells using anti-TRAF6 antibody (A00185). <br> Overlay histogram showing HEPA 1-6 cells stained with A00185 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF6 Antibody (A00185, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cdhr1a-antibody-dz07988-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz07988-cdhr1a-primary-antibodies-if-testing-1.jpgAnti-Zebrafish cdhr1a Antibody IF analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988). <br>
Cdhr1a was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz07988-figure-1.pngAnti-Zebrafish cdhr1a AntibodyEvolutionarily conserved localization of pcdh15 and cdhr1 in photoreceptors predicts interactions linking outer segments and calyceal processes.
A-A”) Confocal microscopy of wildtype 5dpf retinal cryosections probed with cdhr1a antibody (green), Peanut germ agglutinin (PNA - magenta), Wheat germ agglutinin (WG - teal) and pcdh15b (red). White boxes indicate the location of the inset enlargement. White arrowheads highlight the linear localization of cdhr1a along cone OSs (A) and pcdh15b outlining the calyceal process (A’). Merge of all three signals highlights the proximity between cdhr1a and pcdh15b (A”). Scale bar = 10μm. B) Confocal microscopy of wildtype 5dpf retinal cryosections probed with cdhr1a antibody (green), pcdh15b (red) and WGA (teal). White boxes indicate the location of the inset enlargement. White arrowheads highlight the linear localization of cdhr1a along rod OSs (B) and pcdh15b outlining the calyceal process (B’). Merge of all three signals highlights the proximity between cdhr1a and pcdh15b (B”). Scale bar = 10μm. C) Structured illumination microscopy (SIM) of wildtype zebrafish retinal cryosections probed with cdhr1a (green) and pcdh15b (red). White box represents the inset enlargement. White arrowheads highlight the juxtaposition of the cdhr1a and pcdh15b signals. Scale bar = 10μm. D) Diagrammatic model of the connection between the OS discs and CPs in both rod and cone cells mediated by the interaction between OS bound cdhr1a and CP bound pcdh15b. E-L) Structured illumination microscopy (SIM) of wildtype xenopus (E), mallard duck (F), mouse (G), rat (H), spiny mouse (I), gerbil (J), macaque (K) and human (L) retinal sections probed for cdhr1 (green) and pcdh15 (red). White boxes represent the inset enlargements. White arrowheads highlight the juxtaposition of the cdhr1a and pchd15b signals in each species. The model figure was prepared using Biorender. Scale bar = 10μm. <br><b>Index in eLife under a CC BY license. DOI: <a href='https://elifesciences.org/reviewed-preprints/102258v1'>10.7554/eLife.102258.1</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz07988-cdhr1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish cdhr1a Antibody IHC analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988). <br>
Cdhr1a was detected in paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz07988-cdhr1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish cdhr1a Antibody IHC analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988). <br>
Cdhr1a was detected in paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-ugt1a6-picoband-trade-antibody-a02239-1-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-1-ugt1a6-primary-antibodies-wb-testing-1_1.jpgAnti-UGT1A6 Antibody Picoband® Western blot analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A6 antigen affinity purified polyclonal antibody (Catalog # A02239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A6 at approximately 55-61KD. The expected band size for UGT1A6 is at 61KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-1-ugt1a6-primary-antibodies-if-testing-2_1.jpgAnti-UGT1A6 Antibody Picoband® IF analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-1). <br> UGT1A6 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UGT1A6 Antibody (A02239-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-siah1-antibody-dz02095-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02095-siah1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish siah1 Antibody IF analysis of Siah1 using anti-Siah1 antibody (DZ02095). <br>
Siah1 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Siah1 Antibody (DZ02095) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594 Conjugated Avidin (BA1142). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-nlz2-antibody-dz12892-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz12892-nlz2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish nlz2 znf503 Antibody Western blot analysis of nlz2 using anti-nlz2 antibody (DZ12892). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Marker, <br>
Lane 2: Zebrafish tissue lysates, <br>
Lane 3: Zebrafish head tissue lysates, <br>
Lane 4: Zebrafish body tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nlz2 antigen affinity purified polyclonal antibody (DZ12892) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nlz2 at approximately 63 kDa. The expected band size for nlz2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz12892-nlz2-primary-antibodies-if-testing-2.jpgAnti-Zebrafish nlz2 znf503 Antibody IF analysis of nlz2 using anti-nlz2 antibody (DZ12892). <br>
nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody (DZ12892) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ugt1a6-picoband-trade-antibody-a02239-2-boster.html2026-03-24T05:25:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-2-ugt1a6-primary-antibodies-wb-testing-1.jpgAnti-UGT1A6 Antibody Picoband® Western blot analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse SP20 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A6 antigen affinity purified polyclonal antibody (Catalog # A02239-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A6 at approximately 55-61KD. The expected band size for UGT1A6 is at 61KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-2-ugt1a6-primary-antibodies-ihc-testing-3.jpgAnti-UGT1A6 Antibody Picoband®IHC analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-2). <br>UGT1A6 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UGT1A6 Antibody (A02239-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-2-ugt1a6-primary-antibodies-ihc-testing-2.jpgAnti-UGT1A6 Antibody Picoband® IHC analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-2). <br> UGT1A6 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A6 Antibody (A02239-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02239-2-ugt1a6-primary-antibodies-fc-testing-3.pngAnti-UGT1A6 Antibody Picoband® Flow Cytometry analysis of HEPA 1-6 cells using anti-UGT1A6 antibody (A02239-2). <br> Overlay histogram showing HEPA 1-6 cells stained with A02239-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UGT1A6 Antibody (A02239-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alix-pdcd6ip-picoband-trade-antibody-monoclonal-m01751-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-wb-testing-1_5.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) Western blot analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human Jurkat whole cell lysates<br>Lane 4: human PANC-1 whole cell lysates<br>Lane 5: human K562 whole cell lysates<br>Lane 6: human SW579 whole cell lysates<br>Lane 7: rat RH35 whole cell lysates<br>Lane 8: mouse NIH3T3 whole cell lysates<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDCD6IP antigen affinity purified monoclonal antibody (Catalog # M01751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDCD6IP at approximately 96-100KD. The expected band size for PDCD6IP is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-2.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-3.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-4.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-5.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-6.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-ihc-testing-7_1.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-if-testing-8_1.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) IF analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1). <br>PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-1-pdcd6ip-primary-antibodies-fc-testing-9.jpgAnti-ALIX/PDCD6IP Antibody Picoband®(monoclonal, 14D10) Flow Cytometry analysis of A431 cells using anti-PDCD6IPantibody (M01751-1).<br>Overlay histogram showing A431 cells stained with M01751-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDCD6IP Antibody (M01751-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/boster-multiplex-elisa-kits/multiplex-elisa-kit-for-human-cytokine-stripwells-16-plex-mek1001-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_1_.pngMultiplex ELISA Kit For Human Cytokine - Stripwells (16-plex)
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-18-picoband-trade-antibody-monoclonal-m01357-4-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-wb-testing-1_1.jpgAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) Western blot analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (M01357-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hours at RT. The membrane was incubated with mouse anti-Cytokeratin 18 antigen affinity purified monoclonal antibody (Catalog # M01357-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytokeratin 18 at approximately 48KD. The expected band size for Cytokeratin 18 is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (M01357-4). <br> Cytokeratin 18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cytokeratin 18 Antibody (M01357-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) IHC analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (M01357-4). <br> Cytokeratin 18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Cytokeratin 18 Antibody (M01357-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-if-testing-4.jpgAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) IF analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (M01357-4). <br> Cytokeratin 18 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Cytokeratin 18 Antibody (M01357-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-if-testing-5.jpgAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) IF analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody (M01357-4). <br> Cytokeratin 18 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-Cytokeratin 18 Antibody (M01357-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-4-krt18-primary-antibodies-fcm-testing-6.pngAnti-Cytokeratin 18 KRT18 Antibody Picoband®(monoclonal, 7I6) Flow Cytometry analysis of A431 cells using anti- Cytokeratin 18 antibody (M01357-4). <br>Overlay histogram showing A431 cells stained with M01357-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 18 Antibody (M01357-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-tubulin-picoband-trade-antibody-monoclonal-m01857-3-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-3-tubulin_beta-primary-antibodies-wb-testing-1.jpgAnti-Beta Tubulin Antibody Picoband®(monoclonal, 5E4) Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (M01857-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HEPG2 whole cell lysates, <br>
Lane 4: human HL-60 whole cell lysates, <br>
Lane 5: human Raji whole cell lysates, <br>
Lane 6: rat brain tissue lysates, <br>
Lane 7: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Beta Tubulin antigen affinity purified monoclonal antibody (Catalog # M01857-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 55KD. The expected band size for Beta Tubulin is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-3-tubulin_beta-primary-antibodies-if-testing-2.jpgAnti-Beta Tubulin Antibody Picoband®(monoclonal, 5E4) IF analysis of Beta Tubulin using anti-Beta Tubulin antibody (M01857-3). <br>
Beta Tubulin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Beta Tubulin Antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-3-tubulin_beta-primary-antibodies-fcm-testing-3.pngAnti-Beta Tubulin Antibody Picoband®(monoclonal, 5E4) Flow Cytometry analysis of SiHa cells using anti- Beta Tubulin antibody (M01857-3). <br>Overlay histogram showing SiHa cells stained with M01857-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beta Tubulin Antibody (M01857-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zap70-picoband-trade-antibody-monoclonal-m00754-4-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-4-zap70-primary-antibodies-wb-testing-1.jpgAnti-ZAP70 Antibody Picoband®(monoclonal, 6F12) Western blot analysis of ZAP70 using anti-ZAP70 antibody (M00754-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human CCR-CEM whole cell lysates, <br>
Lane 3: mouse spleen tissue lysates, <br>
Lane 4: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ZAP70 antigen affinity purified monoclonal antibody (Catalog # M00754-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70KD. The expected band size for ZAP70 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-4-zap70-primary-antibodies-fcm-testing-2.pngAnti-ZAP70 Antibody Picoband®(monoclonal, 6F12) Flow Cytometry analysis of 293T cells using anti-ZAP70 antibody (M00754-4). <br>Overlay histogram showing 293T cells stained with M00754-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- ZAP70 Antibody (M00754-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptbp1-picoband-trade-antibody-monoclonal-m01600-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-wb-testing-1.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) Western blot analysis of ATF1 using anti-ATF1 antibody (M01600-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human HepG2 whole cell lysates;<br>
Lane 2: human Hela whole cell lysates;<br>
Lane 3: human SW620 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ATF1 antigen affinity purified monoclonal antibody (Catalog # M01600-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ATF1 at approximately 38KD. The expected band size for ATF1 is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-ihc-testing-2.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) IHC analysis of ATF1 using anti-ATF1 antibody (M01600-1). <br>
ATF1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATF1 Antibody (M01600-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-ihc-testing-3.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) IHC analysis of ATF1 using anti-ATF1 antibody (M01600-1). <br>
ATF1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATF1 Antibody (M01600-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-ihc-testing-4.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) IHC analysis of ATF1 using anti-ATF1 antibody (M01600-1). <br>
ATF1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATF1 Antibody (M01600-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-fcm-testing-5.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) Flow Cytometry analysis of K562 cells using anti- ATF1 antibody (M01600-1). <br>Overlay histogram showing K562 cells stained with M01600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ATF1 Antibody (M01600-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01600-1-atf1-primary-antibodies-if-testing-6.jpgAnti-ATF1 Antibody Picoband®(monoclonal, 7F8) IF analysis of ATF1 using anti-ATF1 antibody (M01600-1). <br>
ATF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ATF1 Antibody (M01600-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptbp1-picoband-trade-antibody-monoclonal-m01798-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-wb-testing-1.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) Western blot analysis of PTBP1 using anti-PTBP1 antibody (M01798-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human K562 whole cell lysates;<br>
Lane 3: human A549 whole cell lysates;<br>
Lane 4: human HEK293 whole cell lysates;<br>
Lane 5: human Jurkat whole cell lysates;<br>
Lane 6: human Raji whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PTBP1 antigen affinity purified monoclonal antibody (Catalog # M01798-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PTBP1 at approximately 57KD. The expected band size for PTBP1 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-ihc-testing-2.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IHC analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-ihc-testing-3.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IHC analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-ihc-testing-4.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IHC analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-if-testing-5.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IF analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-if-testing-6.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IF analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-if-testing-7.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) IF analysis of PTBP1 using anti-PTBP1 antibody (M01798-1). <br>
PTBP1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PTBP1 Antibody (M01798-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01798-1-ptbp1-primary-antibodies-fcm-testing-8.jpgAnti-PTBP1 Antibody Picoband®(monoclonal, 3H12) Flow Cytometry analysis of K562 cells using anti- PTBP1 antibody (M01798-1). <br>Overlay histogram showing K562 cells stained with M01798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PTBP1 Antibody (M01798-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd79b-picoband-trade-antibody-monoclonal-m01399-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01399-1-cd79b-primary-antibodies-wb-testing-1.jpgAnti-CD79b Antibody Picoband®(monoclonal, 6H11) Western blot analysis of CD79b using anti-CD79b antibody (M01399-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Raji whole cell lysates;<br>
Lane 2: human Raji whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD79b antigen affinity purified monoclonal antibody (Catalog # M01399-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD79b at approximately 35KD. The expected band size for CD79b is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01399-1-cd79b-primary-antibodies-ihc-testing-2.jpgAnti-CD79b Antibody Picoband®(monoclonal, 6H11) IHC analysis of CD79b using anti-CD79b antibody (M01399-1). <br>
CD79b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01399-1-cd79b-primary-antibodies-ihc-testing-3.jpgAnti-CD79b Antibody Picoband®(monoclonal, 6H11) IHC analysis of CD79b using anti-CD79b antibody (M01399-1). <br>
CD79b was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsc70-picoband-trade-antibody-monoclonal-m01024-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hspa8-primary-antibodies-wb-testing-1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (M01024-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Caco-2 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human Hacat whole cell lysates, <br>
Lane 5: human U251 whole cell lysates, <br>
Lane 6: human THP-1 whole cell lysates, <br>
Lane 7: human SiHa whole cell lysates, <br>
Lane 8: human MCF-7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HSC70/HSPA8 antigen affinity purified monoclonal antibody (M01024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hspa8-primary-antibodies-wb-testing-2.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (M01024-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: rat stomach tissue lysates, <br>
Lane 3: rat brain tissue lysates, <br>
Lane 4: rat RH-35 whole cell lysates, <br>
Lane 5: mouse thymus tissue lysates, <br>
Lane 6: mouse stomach tissue lysates, <br>
Lane 7: mouse brain tissue lysates, <br>
Lane 8: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HSC70/HSPA8 antigen affinity purified monoclonal antibody (M01024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-3_1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-4_1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in paraffin-embedded section of human rectal cancer (lymph node) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-5_1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in paraffin-embedded section of human rectal cancer (lymph node) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-6_1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-7.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-ihc-testing-8.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IHC analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-if-testing-9.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) IF analysis of Hsc70 using anti-Hsc70 antibody (M01024-1). <br>
Hsc70 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Hsc70 Antibody (M01024-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-fcm-testing-10_1.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) Flow Cytometry analysis of A549 cells using anti- Hsc70 antibody (M01024-1). <br>Overlay histogram showing A549 cells stained with M01024-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Hsc70 Antibody (M01024-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-fcm-testing-11.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) Flow Cytometry analysis of K562 cells using anti- Hsc70 antibody (M01024-1). <br>Overlay histogram showing K562 cells stained with M01024-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Hsc70 Antibody (M01024-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01024-1-hsc70-primary-antibodies-fcm-testing-12.jpgAnti-Hsc70 Antibody Picoband®(monoclonal, 3B6) Flow Cytometry analysis of HEPA1-6 cells using anti- Hsc70 antibody (M01024-1). <br>Overlay histogram showing HEPA1-6 cells stained with M01024-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Hsc70 Antibody (M01024-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd59-picoband-trade-antibody-monoclonal-m00914-2-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00914-2-cd59-primary-antibodies-wb-testing-1_1.jpgAnti-CD59 Antibody Picoband®(monoclonal, 3C10) Western blot analysis of CD59 using anti-CD59 antibody (M00914-2).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human U-87MG whole cell lysates;<br>
Lane 3: human PC-3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD59 antigen affinity purified monoclonal antibody (Catalog # M00914-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD59 at approximately 19KD. The expected band size for CD59 is at 19KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00914-2-cd59-primary-antibodies-if-testing-2.jpgAnti-CD59 Antibody Picoband®(monoclonal, 3C10) IF analysis of CD59 using anti-CD59 antibody (M00914-2). <br>
CD59 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CD59 Antibody (M00914-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00914-2-cd59-primary-antibodies-if-testing-3.jpgAnti-CD59 Antibody Picoband®(monoclonal, 3C10) IF analysis of CD59 using anti-CD59 antibody (M00914-2). <br>
CD59 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CD59 Antibody (M00914-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00914-2-cd59-primary-antibodies-fcm-testing-4.jpgAnti-CD59 Antibody Picoband®(monoclonal, 3C10) Flow Cytometry analysis of A549 cells using anti- CD59 antibody (M00914-2). <br>Overlay histogram showing A549 cells stained with M00914-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CD59 Antibody (M00914-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00914-2-cd59-primary-antibodies-ihc-testing-5.jpgAnti-CD59 Antibody Picoband®(monoclonal, 3C10) IHC analysis of CD59 using anti-CD59 antibody (M00914-2). <br>
CD59 was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD59 Antibody (M00914-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-xrcc2-picoband-trade-antibody-a02138-2-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02138-2-xrcc2-primary-antibodies-wb-testing-1.jpgAnti-XRCC2 Antibody Picoband® Western blot analysis of Xrcc2 using anti-Xrcc2 antibody (A02138-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: rat spleen tissue lysates, <br>
Lane 4: mouse spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Xrcc2 antigen affinity purified polyclonal antibody (Catalog # A02138-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Xrcc2 at approximately 36KD. The expected band size for Xrcc2 is at 32KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ttk-mps1-ttk-picoband-trade-antibody-a01132-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01132-1-ttk-primary-antibodies-wb-testing-1.jpgAnti-TTK/Mps1/TTK Antibody Picoband® Western blot analysis of TTK using anti-TTK antibody (A01132-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: rat testicular tissue lysates, <br>
Lane 4: mouse spleen tissue lysates, <br>
Lane 5: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTK antigen affinity purified polyclonal antibody (Catalog # A01132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTK at approximately 97KD. The expected band size for TTK is at 97KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01132-1-ttk-primary-antibodies-ihc-testing-2.jpgAnti-TTK/Mps1/TTK Antibody Picoband® IHC analysis of TTK using anti-TTK antibody (A01132-1). <br>
TTK was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TTK Antibody (A01132-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01132-1-ttk-primary-antibodies-ihc-testing-3.jpgAnti-TTK/Mps1/TTK Antibody Picoband® IHC analysis of TTK using anti-TTK antibody (A01132-1). <br>
TTK was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TTK Antibody (A01132-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01132-1-ttk-primary-antibodies-icc-testing-4.jpgAnti-TTK/Mps1/TTK Antibody Picoband® IF analysis of TTK using anti-TTK antibody (A01132-1). <br>
TTK was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TTK Antibody (A01132-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01132-1-ttk-primary-antibodies-fc-testing-5.pngAnti-TTK/Mps1/TTK Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TTK antibody (A01132-1). <br>Overlay histogram showing U937 cells stained with A01132-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTK Antibody (A01132-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-trim6-antibody-a02016-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-wb-testing-1.jpgAnti-TLR1 TRIM6 Antibody Picoband® Western blot analysis of TRIM6 using anti-TRIM6 antibody (A02016-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human K56 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: rat thymus tissue lysates, <br>
Lane 5: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM6 antigen affinity purified polyclonal antibody (Catalog # A02016-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM6 at approximately 56KD. The expected band size for TRIM6 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-ihc-testing-2.jpgAnti-TLR1 TRIM6 Antibody Picoband® IHC analysis of TRIM6 using anti-TRIM6 antibody (A02016-1). <br>
TRIM6 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM6 Antibody (A02016-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-ihc-testing-3.jpgAnti-TLR1 TRIM6 Antibody Picoband® IHC analysis of TRIM6 using anti-TRIM6 antibody (A02016-1). <br>
TRIM6 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM6 Antibody (A02016-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-ihc-testing-4.jpgAnti-TLR1 TRIM6 Antibody Picoband® IHC analysis of TRIM6 using anti-TRIM6 antibody (A02016-1). <br>
TRIM6 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM6 Antibody (A02016-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-icc-testing-5.jpgAnti-TLR1 TRIM6 Antibody Picoband® IF analysis of TRIM6 using anti-TRIM6 antibody (A02016-1). <br>
TRIM6 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRIM6 Antibody (A02016-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02016-1-trim6-primary-antibodies-fc-testing-6.pngAnti-TLR1 TRIM6 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRIM6 antibody (A02016-1). <br>Overlay histogram showing 293T cells stained with A02016-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM6 Antibody (A02016-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-tlr1-antibody-a00429-3-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-3-tlr1-primary-antibodies-wb-testing-1.jpgAnti-Toll-like receptor 1 TLR1 Antibody Picoband® Western blot analysis of TLR1 using anti-TLR1 antibody (A00429-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human THP-1 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody (Catalog # A00429-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-3-tlr1-primary-antibodies-ihc-testing-3.jpgAnti-Toll-like receptor 1 TLR1 Antibody Picoband®IHC analysis of TLR1 using anti-TLR1 antibody (A00429-3). <br>TLR1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLR1 Antibody (A00429-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-3-tlr1-primary-antibodies-ihc-testing-2.jpgAnti-Toll-like receptor 1 TLR1 Antibody Picoband® IHC analysis of TLR1 using anti-TLR1 antibody (A00429-3). <br>
TLR1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR1 Antibody (A00429-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-3-tlr1-primary-antibodies-fc-testing-3.pngAnti-Toll-like receptor 1 TLR1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-TLR1 antibody (A00429-3). <br>Overlay histogram showing A549 cells stained with A00429-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (A00429-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00429-3-tlr1-primary-antibodies-fc-testing-4.pngAnti-Toll-like receptor 1 TLR1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody (A00429-3). <br>Overlay histogram showing THP-1 cells stained with A00429-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (A00429-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-vmat1-slc18a1-picoband-trade-antibody-a07692-1-boster.html2026-03-24T05:25:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07692-1-slc18a1-primary-antibodies-wb-testing-1.jpgAnti-VMAT1/SLC18A1 Antibody Picoband® Western blot analysis of SLC18A1 using anti-SLC18A1 antibody (A07692-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human HL-60 cell lysates, <br>
Lane 6: human K562 cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A1 antigen affinity purified polyclonal antibody (Catalog # A07692-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A1 at approximately 56KD. The expected band size for SLC18A1 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07692-1-slc18a1-primary-antibodies-ihc-testing-2.jpgAnti-VMAT1/SLC18A1 Antibody Picoband® IHC analysis of SLC18A1 using anti-SLC18A1 antibody (A07692-1). <br>
SLC18A1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC18A1 Antibody (A07692-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07692-1-slc18a1-primary-antibodies-ihc-testing-3.jpgAnti-VMAT1/SLC18A1 Antibody Picoband® IHC analysis of SLC18A1 using anti-SLC18A1 antibody (A07692-1). <br>
SLC18A1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC18A1 Antibody (A07692-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07692-1-slc18a1-primary-antibodies-fc-testing-4.pngAnti-VMAT1/SLC18A1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SLC18A1 antibody (A07692-1). <br>Overlay histogram showing HL-60 cells stained with A07692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC18A1 Antibody (A07692-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-glut9-slc2a9-picoband-trade-antibody-a02245-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-wb-testing-1_1.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® Western blot analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human Hela whole cell lysates, <br>
Lane 7: rat kidney tissue lysates, <br>
Lane 8: rat liver tissue lysates, <br>
Lane 9: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A9 antigen affinity purified polyclonal antibody (Catalog # A02245) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A9 at approximately 59KD. The expected band size for SLC2A9 is at 59KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-ihc-testing-2.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® IHC analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
SLC2A9 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A9 Antibody (A02245) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-ihc-testing-3.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® IHC analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
SLC2A9 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A9 Antibody (A02245) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-ihc-testing-4.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® IHC analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
SLC2A9 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A9 Antibody (A02245) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-ihc-testing-5.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® IHC analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
SLC2A9 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A9 Antibody (A02245) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-icc-testing-6.jpgAnti-GLUT9/SLC2A9 Antibody Picoband® IF analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). <br>
SLC2A9 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC2A9 Antibody (A02245) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02245-slc2a9-primary-antibodies-fc-testing-7.pngAnti-GLUT9/SLC2A9 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SLC2A9 antibody (A02245). <br>Overlay histogram showing U937 cells stained with A02245 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC2A9 Antibody (A02245, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-slbp-picoband-trade-antibody-a02964-2-boster.html2026-03-25T05:23:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02964-2-slbp-primary-antibodies-wb-testing-1.jpgAnti-SLBP Antibody Picoband® Western blot analysis of SLBP using anti-SLBP antibody (A02964-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: mouse spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLBP antigen affinity purified polyclonal antibody (Catalog # A02964-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLBP at approximately 40KD. The expected band size for SLBP is at 31KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02964-2-slbp-primary-antibodies-ihc-testing-2.jpgAnti-SLBP Antibody Picoband® IHC analysis of SLBP using anti-SLBP antibody (A02964-2). <br>
SLBP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLBP Antibody (A02964-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02964-2-slbp-primary-antibodies-icc-testing-3.jpgAnti-SLBP Antibody Picoband® IF analysis of SLBP using anti-SLBP antibody (A02964-2). <br>
SLBP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLBP Antibody (A02964-2) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02964-2-slbp-primary-antibodies-fc-testing-4.pngAnti-SLBP Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SLBP antibody (A02964-2). <br>Overlay histogram showing U87 cells stained with A02964-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLBP Antibody (A02964-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-senp1-picoband-trade-antibody-a02156-3-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02156-3-senp1-primary-antibodies-wb-testing-1_1.jpgAnti-SENP1 Antibody Picoband® Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates, <br>
Lane 2: human HEPG2 whole cell lysates, <br>
Lane 3: human HELA whole cell lysates, <br>
Lane 4: human Jurkat whole cell lysates, <br>
Lane 5: human U87 whole cell lysates, <br>
Lane 6: human Raji whole cell lysates, <br>
Lane 7: human HL-60 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02156-3-senp1-primary-antibodies-fcm-testing-2.pngAnti-SENP1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SENP1 antibody (A02156-3). <br>Overlay histogram showing A549 cells stained with A02156-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SENP1 Antibody (A02156-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rictor-picoband-trade-antibody-a03195-1-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03195-1-rictor-primary-antibodies-wb-testing-1_1.jpgAnti-RICTOR Antibody Picoband® Western blot analysis of RICTOR using anti-RICTOR antibody (A03195-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RICTOR antigen affinity purified polyclonal antibody (Catalog # A03195-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RICTOR at approximately 230KD. The expected band size for RICTOR is at 192KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03195-1-rictor-primary-antibodies-ihc-testing-2.jpgAnti-RICTOR Antibody Picoband® IHC analysis of RICTOR using anti-RICTOR antibody (A03195-1). <br>
RICTOR was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RICTOR Antibody (A03195-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03195-1-rictor-primary-antibodies-ihc-testing-3.jpgAnti-RICTOR Antibody Picoband® IHC analysis of RICTOR using anti-RICTOR antibody (A03195-1). <br>
RICTOR was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RICTOR Antibody (A03195-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03195-1-rictor-primary-antibodies-icc-testing-4.jpgAnti-RICTOR Antibody Picoband® IF analysis of RICTOR using anti-RICTOR antibody (A03195-1). <br>
RICTOR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RICTOR Antibody (A03195-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03195-1-rictor-primary-antibodies-fc-testing-5.pngAnti-RICTOR Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-RICTOR antibody (A03195-1). <br>Overlay histogram showing 293T cells stained with A03195-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RICTOR Antibody (A03195-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pms2-picoband-trade-antibody-a01028-1-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01028-1-pms2-primary-antibodies-wb-testing-1.jpgAnti-PMS2 Antibody Picoband® Western blot analysis of Pms2 using anti-Pms2 antibody (A01028-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat kidney tissue lysates, <br>
Lane 2: mouse kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pms2 antigen affinity purified polyclonal antibody (Catalog # A01028-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pms2 at approximately 120KD. The expected band size for Pms2 is at 96KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cd31-pecam1-picoband-trade-antibody-a02995-2-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02995-2-plscr1-primary-antibodies-wb-testing-1_1.jpgAnti-Scramblase 1/PLSCR1 Antibody Picoband® Western blot analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human A431 whole cell lysates, <br>
Lane 4: human 293T whole cell lysates, <br>
Lane 5: mouse lung tissue lysates, <br>
Lane 6: mouse RAW264.7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLSCR1 antigen affinity purified polyclonal antibody (Catalog # A02995-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLSCR1 at approximately 35 kDa. The expected band size for PLSCR1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02995-2-plscr1-primary-antibodies-ihc-testing-2.jpgAnti-Scramblase 1/PLSCR1 Antibody Picoband® IHC analysis of PLSCR1 using anti-PLSCR1 antibody (A02995-2). <br>
PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLSCR1 Antibody (A02995-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-cd31-pecam1-picoband-trade-antibody-a01513-3-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-pecam1-primary-antibodies-wb-testing-1.jpgAnti-CD31/Pecam1 Antibody Picoband® Western blot analysis of Pecam1 using anti-Pecam1 antibody (A01513-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pecam1 antigen affinity purified polyclonal antibody (Catalog # A01513-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pecam1 at approximately 120-130KD. The expected band size for Pecam1 is at 82KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-13048_2025_1679_fig4_html.pngAnti-CD31/Pecam1 Antibody Picoband®YJD affected angiogenesis and oxidative stress in POF rats. ( A ) IHC was performed to detect the expression of CD31 and α-SMA, which is located around growing follicles in the ovaries; Black arrows point to areas of positive staining. ( B ) Average optical density of CD31. ( C ) The average optical density of α-SMA. ( D – H ) Serum levels of the hormones P, E2, FSH, LH, and AMH were examined by ELISA. ( I – J ) Serum levels of indicators of oxidative stress, MDA and SOD, were assessed by ELISA. *** P < 0.001 vs. Control; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. TP; & P < 0.05, && P < 0.01, &&& P < 0.001 vs. TP + M-YJD <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-025-01679-2'>40287776</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-41598_2023_48897_fig4_html.pngAnti-CD31/Pecam1 Antibody Picoband®Light flicker stimulation at 40 Hz does not increase angiogenesis in mice with rUCCAO. ( a , b ) CD31 immunohistochemical staining in the frontal lobe and hippocampus. Scale bar = 50 μm; the blue arrow indicates the positive area. ( c – f ) Positive area (%) and IOD of CD31 protein in the frontal lobe and hippocampus, respectively (n = 3/group. Means ± SEMs, *p < 0.05, n.s. not significant). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-023-48897-6'>38049571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-ajtr0009-1460-f4.jpgAnti-CD31/Pecam1 Antibody Picoband®Observation for the IL-27 + -CD31 + double-positive staining cells in health control group, periapical granulomas group and Radicular cysts group. A. The IL-27 + , CD31 + and DAPI + positive stained cells, and the merged stained cells were illustrated in the images. B. Statistical analysis of the densities of IL-27 + -CD31 + double-positive cells in every group. *P<0.05 represents the values in periapical granulomas group and radicular group compared to health control group. #P<0.05 represents the value in radicular group compared to periapical granulomas group. The scale bars have been illustrated in the images with the original magnification, ×400.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5376036/&orig_host=www.ncbi.nlm.nih.gov'>28386371</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-ajtr0009-1460-f5.jpgAnti-CD31/Pecam1 Antibody Picoband®Observation for the IL-27 + -tryptase + , IL-27 + -CD14 + and IL-27 + -CD31 + double staining results in periapical cyst group by using laser scanning confocal microscope. The scale bars have been illustrated in the images with the original magnification, ×1260.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5376036/&orig_host=www.ncbi.nlm.nih.gov'>28386371</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-fgene-16-1646991-g007.jpgAnti-CD31/Pecam1 Antibody Picoband®Construction of animal models and analysis of pathological damage. (A) TTC staining plots of the Sham and VD group. (B) Motion trajectory diagram. (C) Representative images stained with HE (scale bars = 50 μm). (D) Representative histopathological images obtained from TUNEL staining (scale bars = 50 μm), showing changes in the cortex hippocampus CA1. (E) Quantification of TUNEL+ cells in the hippocampal CA1 (n = 3). (F) Expression profile of CD31 between Sham and VD groups (qPCR). **P < 0.01. (G) Expression profile of HIF-1α between Sham and VD groups (qPCR; n = 6). ***P < 0.001. (H) WB strips. (I) Expression profile of CD31 between Sham and VD groups (WB). *P < 0.05. (J) Expression profile of HIF-1α between Sham and VD groups (WB; n = 3). ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1646991/full'>40988927</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-fgene-16-1646991-g008.jpgAnti-CD31/Pecam1 Antibody Picoband®Experimental validation of the key genes in vivo . (A) Representative images of immunofluorescence. (B–F) Expression profile of CCL2, VEGFA, SPP1, ANGPT2, and ANGPTL4 between Sham and VD groups (qPCR; n = 6). *P < 0.05; **P < 0.01; ***P < 0.001. (G) Immunofluorescence quantification of CD31. ***P < 0.001. (H) WB representative strips of the 5 key genes. (I–M) Protein expression level of CCL2, VEGFA, SPP1, ANGPT2, and ANGPTL4 between Sham and VD groups (WB; n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1646991/full'>40988927</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-pecam1-primary-antibodies-ihc-testing-2.jpgAnti-CD31/Pecam1 Antibody Picoband® IHC analysis of Pecam1 using anti-Pecam1 antibody (A01513-3). <br>
Pecam1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Pecam1 Antibody (A01513-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-pecam1-primary-antibodies-ihc-testing-3.jpgAnti-CD31/Pecam1 Antibody Picoband® IHC analysis of Pecam1 using anti-Pecam1 antibody (A01513-3). <br>
Pecam1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Pecam1 Antibody (A01513-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-pecam1-primary-antibodies-ihc-testing-4.jpgAnti-CD31/Pecam1 Antibody Picoband® IHC analysis of Pecam1 using anti-Pecam1 antibody (A01513-3). <br>
Pecam1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Pecam1 Antibody (A01513-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-3-yongqiu_zhen.pngAnti-CD31/Pecam1 Antibody Picoband®IF analysis of Pecam1 using anti-Pecam1 antibody (A01513-3). <br>
Pecam1 was detected in a paraffin-embedded section of human normal liver and alcoholic liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Pecam1 Antibody (A01513-3) overnight at 4°C. DyLight 594 Donkey Anti-Mouse IgG (H+L) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd31-pecam1-picoband-trade-antibody-a01513-2-boster.html2026-03-25T05:23:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-wb-testing-1.jpgAnti-CD31/Pecam1 Antibody Picoband®Western blot analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: rat heart tissue lysates, <br>
Lane 4: mouse lung tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/Pecam1 antigen affinity purified polyclonal antibody (A01513-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD31/Pecam1 at approximately 100-120 kDa. The expected band size for CD31/Pecam1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-1.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-2.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-3.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-4.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-5.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-6.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-7.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-if-testing-1.pngAnti-CD31/Pecam1 Antibody Picoband®IF analysis of CD31 using anti-CD31 antibody (A01513-2).<br>
Paraffin-embedded mouse skin sections were stained for CD31 in three experimental groups: (1) normal control, (2) congestion group, in which the skin was treated with ethanol, and (3) inflammation group, stimulated with LPS. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD31 Antibody (A01513-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody incubated with 1:500 for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-ihc-testing-8.jpgAnti-CD31/Pecam1 Antibody Picoband®IHC analysis of CD31/Pecam1 using anti-CD31/Pecam1 antibody (A01513-2). <br>CD31/Pecam1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/Pecam1 Antibody (A01513-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01513-2-cd31-primary-antibodies-if-testing-2.pngAnti-CD31/Pecam1 Antibody Picoband®IF analysis of CD31 using anti-CD31 antibody (A01513-2).<br>
CD31/Pecam1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD31 Antibody (A01513-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody incubated with 1:500 for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-lox-1-olr1-picoband-trade-antibody-a00760-3-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00760-3-olr1-primary-antibodies-wb-testing-1_1.jpgAnti-LOX 1/Olr1 Antibody Picoband® Western blot analysis of Olr1 using anti-Olr1 antibody (A00760-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mouse brain tissue lysates, <br>
Lane 2: mouse liver tissue lysates, <br>
Lane 3: mouse kidney tissue lysates, <br>
Lane 4: mouse smooth muscle tissue lysates, <br>
Lane 5: mouse SP2/0 whole cell lysates, <br>
Lane 6: mouse Neuro-2a whole cell lysates, <br>
Lane 7: rat brain tissue lysates, <br>
Lane 8: rat liver tissue lysates, <br>
Lane 9: rat kidney tissue lysates, <br>
Lane 10: rat smooth muscle tissue lysates, <br>
Lane 11: rat C6 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olr1 antigen affinity purified polyclonal antibody (Catalog # A00760-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olr1 at approximately 52 kDa. The expected band size for Olr1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00760-3-olr1-primary-antibodies-ihc-testing-2.jpgAnti-LOX 1/Olr1 Antibody Picoband® IHC analysis of Olr1 using anti-Olr1 antibody (A00760-3). <br>
Olr1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Olr1 Antibody (A00760-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00760-3-olr1-primary-antibodies-fc-testing-3.pngAnti-LOX 1/Olr1 Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-Olr1 antibody (A00760-3). <br>Overlay histogram showing Neuro-2a cells stained with A00760-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Olr1 Antibody (A00760-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lox-1-olr1-picoband-trade-antibody-a00760-2-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00760-2-olr1-primary-antibodies-wb-testing-1.jpgAnti-LOX 1/Olr1 Antibody Picoband® Western blot analysis of Olr1 using anti-Olr1 antibody (A00760-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: mouse brain tissue lysates, <br>
Lane 2: mouse lung tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
Lane 4: mouse kidney tissue lysates, <br>
Lane 5: mouse testicular tissue lysates, <br>
Lane 6: mouse smooth muscle tissue lysates, <br>
Lane 7: mouse SP20 whole cell lysates, <br>
Lane 8: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Olr1 antigen affinity purified polyclonal antibody (Catalog # A00760-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Olr1 at approximately 52KD. The expected band size for Olr1 is at 31KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ofd1-picoband-trade-antibody-a02955-1-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02955-1-ofd1-primary-antibodies-wb-testing-1_1.jpgAnti-OFD1 Antibody Picoband® Western blot analysis of OFD1 using anti-OFD1 antibody (A02955-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OFD1 antigen affinity purified polyclonal antibody (Catalog # A02955-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OFD1 at approximately 117 kDa. The expected band size for OFD1 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02955-1-ofd1-primary-antibodies-if-testing-2.jpgAnti-OFD1 Antibody Picoband® IF analysis of OFD1 using anti-OFD1 antibody (A02955-1). <br>
OFD1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OFD1 Antibody (A02955-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02955-1-ofd1-primary-antibodies-fcm-testing-3.jpgAnti-OFD1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-OFD1 antibody (A02955-1). <br>
Overlay histogram showing JK cells stained with A02955-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OFD1 Antibody (A02955-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ndrg1-picoband-trade-antibody-a01327-1-boster.html2026-03-24T05:25:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01327-1-ndrg1-primary-antibodies-wb-testing-1.jpgAnti-NDRG1 Antibody Picoband® Western blot analysis of NDRG1 using anti-NDRG1 antibody (A01327-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified polyclonal antibody (Catalog # A01327-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 46KD. The expected band size for NDRG1 is at 46KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01327-1-ndrg1-primary-antibodies-ihc-testing-1.jpgAnti-NDRG1 Antibody Picoband®IHC analysis of NDRG1 using anti-NDRG1 antibody (A01327-1). <br>NDRG1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDRG1 Antibody (A01327-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01327-1-ndrg1-primary-antibodies-ihc-testing-2.jpgAnti-NDRG1 Antibody Picoband®IHC analysis of NDRG1 using anti-NDRG1 antibody (A01327-1). <br>NDRG1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDRG1 Antibody (A01327-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01327-1-ndrg1-primary-antibodies-fc-testing-2.pngAnti-NDRG1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-NDRG1 antibody (A01327-1). <br>Overlay histogram showing PC-3 cells stained with A01327-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDRG1 Antibody (A01327-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-morc3-picoband-trade-antibody-a06075-4-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-4-morc3-primary-antibodies-wb-testing-1.jpgAnti-MORC3 Antibody Picoband® Western blot analysis of MORC3 using anti-MORC3 antibody (A06075-4).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat heart tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MORC3 antigen affinity purified polyclonal antibody (Catalog # A06075-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MORC3 at approximately 120KD. The expected band size for MORC3 is at 107KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-4-morc3-primary-antibodies-ihc-testing-2.jpgAnti-MORC3 Antibody Picoband® IHC analysis of MORC3 using anti-MORC3 antibody (A06075-4). <br>
MORC3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MORC3 Antibody (A06075-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-4-morc3-primary-antibodies-ihc-testing-3.jpgAnti-MORC3 Antibody Picoband® IHC analysis of MORC3 using anti-MORC3 antibody (A06075-4). <br>
MORC3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MORC3 Antibody (A06075-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-4-morc3-primary-antibodies-icc-testing-4.jpgAnti-MORC3 Antibody Picoband® IF analysis of MORC3 using anti-MORC3 antibody (A06075-4). <br>
MORC3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MORC3 Antibody (A06075-4) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-4-morc3-primary-antibodies-fc-testing-5.pngAnti-MORC3 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-MORC3 antibody (A06075-4). <br>Overlay histogram showing A431 cells stained with A06075-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MORC3 Antibody (A06075-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mc4-r-mc4r-antibody-a00619-2-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00619-2-mc4r-primary-antibodies-wb-testing-1.jpgAnti-MC4-R/MC4R Antibody Picoband® Western blot analysis of MC4R using anti-MC4R antibody (A00619-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: rat PC-12 whole cell lysates, <br>
Lane 4: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC4R antigen affinity purified polyclonal antibody (Catalog # A00619-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC4R at approximately 37KD. The expected band size for MC4R is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00619-2-mc4r-primary-antibodies-ihc-testing-2.jpgAnti-MC4-R/MC4R Antibody Picoband® IHC analysis of MC4R using anti-MC4R antibody (A00619-2). <br>
MC4R was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MC4R Antibody (A00619-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00619-2-mc4r-primary-antibodies-icc-testing-3.jpgAnti-MC4-R/MC4R Antibody Picoband® IF analysis of MC4R using anti-MC4R antibody (A00619-2). <br>
MC4R was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MC4R Antibody (A00619-2) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00619-2-mc4r-primary-antibodies-fc-testing-4.pngAnti-MC4-R/MC4R Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-MC4R antibody (A00619-2). <br>Overlay histogram showing U937 cells stained with A00619-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MC4R Antibody (A00619-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-masp2-picoband-trade-antibody-a02323-1-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02323-1-masp2-primary-antibodies-wb-testing-1.jpgAnti-MASP2 Antibody Picoband® Western blot analysis of MASP2 using anti-MASP2 antibody (A02323-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASP2 antigen affinity purified polyclonal antibody (Catalog # A02323-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASP2 at approximately 76KD. The expected band size for MASP2 is at 76KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02323-1-masp2-primary-antibodies-fc-testing-2.pngAnti-MASP2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-MASP2 antibody (A02323-1). <br>Overlay histogram showing A431 cells stained with A02323-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MASP2 Antibody (A02323-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02323-1-masp2-primary-antibodies-fc-testing-3.pngAnti-MASP2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-MASP2 antibody (A02323-1). <br>Overlay histogram showing HepG2 cells stained with A02323-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MASP2 Antibody (A02323-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-irf1-picoband-trade-antibody-a00580-1-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00580-1-irf1-primary-antibodies-wb-testing-1_1.jpgAnti-IRF1 Antibody Picoband® Western blot analysis of IRF1 using anti-IRF1 antibody (A00580-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A431 whole cell lysates,<br>
Lane 2: human Hacat whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF1 antigen affinity purified polyclonal antibody (Catalog # A00580-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF1 at approximately 48-50 kDa. The expected band size for IRF1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00580-1-irf1-primary-antibodies-ihc-testing-2.jpgAnti-IRF1 Antibody Picoband® IHC analysis of IRF1 using anti-IRF1 antibody (A00580-1). <br>
IRF1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRF1 Antibody (A00580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00580-1-irf1-primary-antibodies-ihc-testing-3.jpgAnti-IRF1 Antibody Picoband® IHC analysis of IRF1 using anti-IRF1 antibody (A00580-1). <br>
IRF1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRF1 Antibody (A00580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00580-1-irf1-primary-antibodies-icc-testing-4.jpgAnti-IRF1 Antibody Picoband® IF analysis of IRF1 using anti-IRF1 antibody (A00580-1). <br>
IRF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IRF1 Antibody (A00580-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00580-1-irf1-primary-antibodies-fc-testing-5.pngAnti-IRF1 Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-IRF1 antibody (A00580-1). <br>Overlay histogram showing HeLa cells stained with A00580-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF1 Antibody (A00580-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-irak2-picoband-trade-antibody-a01559-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-wb-testing-1_1.jpgAnti-IRAK2 Antibody Picoband® Western blot analysis of IRAK2 using anti-IRAK2 antibody (A01559). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human MOLT-4 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: rat thymus tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse thymus tissue lysates,<br>
Lane 8: mouse RAW264.7 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK2 antigen affinity purified polyclonal antibody (Catalog # A01559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRAK2 at approximately 69 kDa. The expected band size for IRAK2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-ihc-testing-4.jpgAnti-IRAK2 Antibody Picoband®IHC analysis of IRAK2 using anti-IRAK2 antibody (A01559). <br>IRAK2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRAK2 Antibody (A01559) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-ihc-testing-2.jpgAnti-IRAK2 Antibody Picoband® IHC analysis of IRAK2 using anti-IRAK2 antibody (A01559). <br>
IRAK2 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRAK2 Antibody (A01559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-ihc-testing-3.jpgAnti-IRAK2 Antibody Picoband® IHC analysis of IRAK2 using anti-IRAK2 antibody (A01559). <br>
IRAK2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IRAK2 Antibody (A01559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-fc-testing-4.pngAnti-IRAK2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-IRAK2 antibody (A01559). <br>Overlay histogram showing U937 cells stained with A01559 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRAK2 Antibody (A01559, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01559-irak2-primary-antibodies-if-testing-5.jpgAnti-IRAK2 Antibody Picoband® IF analysis of IRAK2 using anti-IRAK2 antibody (A01559). <br>
IRAK2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IRAK2 Antibody (A01559) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-znfn1a2-helios-ikzf2-picoband-trade-antibody-a05228-3-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05228-3-ikzf2-primary-antibodies-wb-testing-1_1.jpgAnti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® Western blot analysis of IKZF2 using anti-IKZF2 antibody (A05228-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human K562 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human Raji whole cell lysates,<br>
Lane 5: rat spleen tissue lysates,<br>
Lane 6: mouse ANA-1 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF2 antigen affinity purified polyclonal antibody (Catalog # A05228-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKZF2 at approximately 70 kDa. The expected band size for IKZF2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05228-3-ikzf2-primary-antibodies-fc-testing-2.pngAnti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-IKZF2 antibody (A05228-3). <br>Overlay histogram showing HL-60 cells stained with A05228-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF2 Antibody (A05228-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fibrinogen-alpha-chain-fga-picoband-trade-antibody-a00816-3-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fga-primary-antibodies-wb-testing-1.jpgAnti-Fibrinogen alpha chain/FGA Antibody Picoband® Western blot analysis of FGA using anti-FGA antibody (A00816-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human T-47D whole cell lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human SW620 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGA antigen affinity purified polyclonal antibody (Catalog # A00816-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGA at approximately 95KD. The expected band size for FGA is at 95KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fonc-11-621806-g007.jpgAnti-Fibrinogen alpha chain/FGA Antibody Picoband®Immunohistochemistry of FGA, F2, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CTH, CFHR3, ENNP1, and NAT2 in clinical GBC and control specimens.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.621806/full'>33718182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fonc-11-621806-g005.jpgAnti-Fibrinogen alpha chain/FGA Antibody Picoband®Survival analysis of the core genes in GBC patients through TCGA database. (A) survival analysis of FGA. (B) survival analysis of CFH. (C) survival analysis of ENPP1. (D) survival analysis of CFHR3. (E) survival analysis of ITIH4. (F) survival analysis of NAT2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.621806/full'>33718182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fonc-11-621806-g006.jpgAnti-Fibrinogen alpha chain/FGA Antibody Picoband®The correlation coefficients among 17 core genes from the TCGA CHOL dataset. (A) the details of correlation coefficients by Pearson statistical analysis among 17 core genes. (B) the genes positively correlated with FGA and CFH .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.621806/full'>33718182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fga-primary-antibodies-ihc-testing-2.jpgAnti-Fibrinogen alpha chain/FGA Antibody Picoband® IHC analysis of FGA using anti-FGA antibody (A00816-3). <br>
FGA was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FGA Antibody (A00816-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fga-primary-antibodies-fc-testing-3.pngAnti-Fibrinogen alpha chain/FGA Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-FGA antibody (A00816-3). <br>Overlay histogram showing HepG2 cells stained with A00816-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGA Antibody (A00816-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00816-3-fga-primary-antibodies-fc-testing-4.pngAnti-Fibrinogen alpha chain/FGA Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-FGA antibody (A00816-3). <br>Overlay histogram showing SiHa cells stained with A00816-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGA Antibody (A00816-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fas-picoband-trade-antibody-a00055-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00055-fas-primary-antibodies-wb-testing-1.jpgAnti-Fas Antibody Picoband® Western blot analysis of Fas using anti-Fas antibody (A00055).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fas antigen affinity purified polyclonal antibody (Catalog # A00055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fas at approximately 37KD. The expected band size for Fas is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00055-fas-primary-antibodies-fc-testing-2_1.pngAnti-Fas Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Fas antibody (A00055). <br>Overlay histogram showing RAW264.7 cells stained with A00055 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fas Antibody (A00055, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-par1-thrombin-receptor-f2r-picoband-trade-antibody-a03352-2-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-2-f2r-primary-antibodies-wb-testing-1.jpgAnti-PAR1/Thrombin Receptor/F2r Antibody Picoband® Western blot analysis of F2r using anti-F2r antibody (A03352-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2r antigen affinity purified polyclonal antibody (Catalog # A03352-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2r at approximately 47KD. The expected band size for F2r is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-2-f2r-primary-antibodies-fc-testing-2.pngAnti-PAR1/Thrombin Receptor/F2r Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-F2r antibody (A03352-1). <br>Overlay histogram showing RH35 cells stained with A03352-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-F2r Antibody (A03352-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-par1-thrombin-receptor-f2r-picoband-trade-antibody-a03352-1-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-f2r-primary-antibodies-wb-testing-1.jpgAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband® Western blot analysis of F2R using anti-F2R antibody (A03352-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human U-87MG whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2R antigen affinity purified polyclonal antibody (Catalog # A03352-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2R at approximately 47KD. The expected band size for F2R is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-fphar-14-1282943-g005.jpgAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband®(A) The binding energy between the six most important targets and the core compounds was calculated via molecular docking models. (B) Piperlongumine and PTGS2, binding energy = −8.56 kcal/mol. (C) Piperlongumine and F2R, binding energy = −8.81 kcal/mol. (D) Piperine and F2R, binding energy = −8.74 kcal/mol.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10847597/&orig_host=www.ncbi.nlm.nih.gov'>38328576</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-fphar-14-1282943-g013.jpgAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband® Cytokine levels in the knee cartilage tissue of 100 and 300 mg/kg PLE-treated rats. (A–L) mRNA expression of F2R, F3, IL-1β, IL-6, IL-17A, MMP-1, MMP-2, MMP-3, MMP-9, MMP-13, NOS2, PTGS2, PGE2, and TNF-α determined by qRT-PCR. (M) WB analysis of the protein expression of F2R, F3, IL-17A, MMP-1, MMP-2, MMP-9, and PTGS2. ### p < 0.001 vs. NT, ** p < 0.05 vs. MIA, *** p < 0.001 vs. MIA. NT: non-treated, INDO 3: indomethacin 3 mg/kg.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10847597/&orig_host=www.ncbi.nlm.nih.gov'>38328576</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-f2r-primary-antibodies-ihc-testing-2.jpgAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband® IHC analysis of F2R using anti-F2R antibody (A03352-1). <br>
F2R was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-F2R Antibody (A03352-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-f2r-primary-antibodies-ihc-testing-3.jpgAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband® IHC analysis of F2R using anti-F2R antibody (A03352-1). <br>
F2R was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-F2R Antibody (A03352-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03352-1-f2r-primary-antibodies-fc-testing-4.pngAnti-PAR1/Thrombin Receptor/F2R Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-F2R antibody (A03352-1). <br>Overlay histogram showing HL-60 cells stained with A03352-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-F2R Antibody (A03352-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-eph-receptor-b2-ephb2-picoband-trade-antibody-a01507-1-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01507-1-ephb2-primary-antibodies-wb-testing-1.jpgAnti-Eph receptor B2/EPHB2 Antibody Picoband® Western blot analysis of EPHB2 using anti-EPHB2 antibody (A01507-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB2 antigen affinity purified polyclonal antibody (Catalog # A01507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB2 at approximately 117KD. The expected band size for EPHB2 is at 117KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01507-1-ephb2-primary-antibodies-icc-testing-2.jpgAnti-Eph receptor B2/EPHB2 Antibody Picoband® IF analysis of EPHB2 using anti-EPHB2 antibody (A01507-1). <br>
EPHB2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EPHB2 Antibody (A01507-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01507-1-ephb2-primary-antibodies-fc-testing-3.pngAnti-Eph receptor B2/EPHB2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-EPHB2 antibody (A01507-1). <br>Overlay histogram showing A549 cells stained with A01507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHB2 Antibody (A01507-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01507-1-ephb2-primary-antibodies-fc-testing-4.pngAnti-Eph receptor B2/EPHB2 Antibody Picoband® Flow Cytometry analysis of Ana-1 cells using anti-EPHB2 antibody (A01507-1). <br>Overlay histogram showing Ana-1 cells stained with A01507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHB2 Antibody (A01507-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01507-1-ephb2-primary-antibodies-fc-testing-5.pngAnti-Eph receptor B2/EPHB2 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-EPHB2 antibody (A01507-1). <br>
Overlay histogram showing C6 cells stained with A01507-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHB2 Antibody (A01507-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-eif4a1-picoband-trade-antibody-a03922-3-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-wb-testing-1_1.jpgAnti-EIF4A1 Antibody Picoband® Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human MCF-7 whole cell lysates,<br>
Lane 5: rat stomach tissue lysates,<br>
Lane 6: rat spleen tissue lysates,<br>
Lane 7: mouse spleen tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A1 antigen affinity purified polyclonal antibody (Catalog # A03922-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4A1 at approximately 46 kDa. The expected band size for EIF4A1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-ihc-testing-2.jpgAnti-EIF4A1 Antibody Picoband® IHC analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
EIF4A1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A1 Antibody (A03922-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-ihc-testing-3.jpgAnti-EIF4A1 Antibody Picoband® IHC analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
EIF4A1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A1 Antibody (A03922-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-ihc-testing-4.jpgAnti-EIF4A1 Antibody Picoband® IHC analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
EIF4A1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF4A1 Antibody (A03922-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-icc-testing-5.jpgAnti-EIF4A1 Antibody Picoband® IF analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
EIF4A1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EIF4A1 Antibody (A03922-3) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-fc-testing-6.pngAnti-EIF4A1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-EIF4A1 antibody (A03922-3). <br>Overlay histogram showing HepG2 cells stained with A03922-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4A1 Antibody (A03922-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03922-3-eif4a1-primary-antibodies-wb-testing-7.jpgAnti-EIF4A1 Antibody Picoband® Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (A03922-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U251 whole cell lysates, <br>
Lane 2: human 293T whole cell lysates, <br>
Lane 3: human MCF-7 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A1 antigen affinity purified polyclonal antibody (A03922-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for EIF4A1 at approximately 46 kDa. The expected band size for EIF4A1 is at 46 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-drd4-picoband-trade-antibody-a00998-2-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00998-2-drd4-primary-antibodies-wb-testing-1.jpgAnti-DRD4 Antibody Picoband® Western blot analysis of Drd4 using anti-Drd4 antibody (A00998-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Drd4 antigen affinity purified polyclonal antibody (Catalog # A00998-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Drd4 at approximately 48KD. The expected band size for Drd4 is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00998-2-drd4-primary-antibodies-fc-testing-2.pngAnti-DRD4 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Drd4 antibody (A00998-2). <br>Overlay histogram showing C6 cells stained with A00998-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Drd4 Antibody (A00998-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ddb2-picoband-trade-antibody-a01430-2-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01430-2-ddb2-primary-antibodies-wb-testing-1_1.jpgAnti-DDB2 Antibody Picoband® Western blot analysis of DDB2 using anti-DDB2 antibody (A01430-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human A431 whole cell lysates,<br>
Lane 3: human Raji whole cell lysates,<br>
Lane 4: human HL-60 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # A01430-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01430-2-ddb2-primary-antibodies-fcm-testing-2.jpgAnti-DDB2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-DDB2 antibody (A01430-2). <br>
Overlay histogram showing U87 cells stained with A01430-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDB2 Antibody (A01430-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-chd2-picoband-trade-antibody-a04079-2-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04079-2-chd2-primary-antibodies-wb-testing-1_1.jpgAnti-CHD2 Antibody Picoband® Western blot analysis of CHD2 using anti-CHD2 antibody (A04079-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: monkey COS-7 whole cell lysates, <br>
Lane 2: human Siha whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD2 antigen affinity purified polyclonal antibody (Catalog # A04079-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD2 at approximately 250 kDa. The expected band size for CHD2 is at 211 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04079-2-chd2-primary-antibodies-fc-testing-2.pngAnti-CHD2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CHD2 antibody (A04079-2). <br>Overlay histogram showing SiHa cells stained with A04079-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHD2 Antibody (A04079-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-choline-acetyltransferase-chat-picoband-trade-antibody-a01192-5-boster.html2026-03-24T05:26:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-5-chat-primary-antibodies-wb-testing-1_1.jpgAnti-Choline Acetyltransferase/Chat Antibody Picoband®Western blot analysis of Chat using anti-Chat antibody (A01192-5). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates,<br>
Lane 2: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chat antigen affinity purified polyclonal antibody (A01192-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Chat at approximately 71 kDa. The expected band size for Chat is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-5-chat-primary-antibodies-ihc-testing-1.jpgAnti-Choline Acetyltransferase/Chat Antibody Picoband®IHC analysis of Chat using anti-Chat antibody (A01192-5). <br>Chat was detected in a paraffin-embedded section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chat Antibody (A01192-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-5-chat-primary-antibodies-ihc-testing-2_1.jpgAnti-Choline Acetyltransferase/Chat Antibody Picoband®IHC analysis of Chat using anti-Chat antibody (A01192-5). <br>Chat was detected in a paraffin-embedded section of rat spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chat Antibody (A01192-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-choline-acetyltransferase-chat-picoband-trade-antibody-a01192-4-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-4-chat-primary-antibodies-wb-testing-1.jpgAnti-Choline Acetyltransferase/CHAT Antibody Picoband® Western blot analysis of CHAT using anti-CHAT antibody (A01192-4).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human placenta whole cell lysates, <br>
Lane 2: human U-87MG tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody (Catalog # A01192-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHAT at approximately 83KD. The expected band size for CHAT is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-4-chat-primary-antibodies-ihc-testing-3.jpgAnti-Choline Acetyltransferase/CHAT Antibody Picoband®IHC analysis of CHAT using anti-CHAT antibody (A01192-4). <br>CHAT was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHAT Antibody (A01192-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-4-chat-primary-antibodies-ihc-testing-2.jpgAnti-Choline Acetyltransferase/CHAT Antibody Picoband® IHC analysis of CHAT using anti-CHAT antibody (A01192-4). <br>
CHAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHAT Antibody (A01192-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-4-chat-primary-antibodies-icc-testing-3.jpgAnti-Choline Acetyltransferase/CHAT Antibody Picoband® IF analysis of CHAT using anti-CHAT antibody (A01192-4). <br>
CHAT was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CHAT Antibody (A01192-4) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-4-chat-primary-antibodies-fc-testing-4.pngAnti-Choline Acetyltransferase/CHAT Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CHAT antibody (A01192-4). <br>Overlay histogram showing SiHa cells stained with A01192-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHAT Antibody (A01192-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pctaire1-cdk16-picoband-trade-antibody-a06102-1-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06102-1-cdk16-primary-antibodies-wb-testing-1.jpgAnti-PCTAIRE1/CDK16 Antibody Picoband® Western blot analysis of CDK16 using anti-CDK16 antibody (A06102-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Caco-2 whole cell lysates, <br>
Lane 2: rat PC-12 whole cell lysates, <br>
Lane 3: mouse NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK16 antigen affinity purified polyclonal antibody (Catalog # A06102-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK16 at approximately 56KD. The expected band size for CDK16 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06102-1-cdk16-primary-antibodies-ihc-testing-2.jpgAnti-PCTAIRE1/CDK16 Antibody Picoband® IHC analysis of CDK16 using anti-CDK16 antibody (A06102-1). <br>
CDK16 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDK16 Antibody (A06102-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06102-1-cdk16-primary-antibodies-fc-testing-3.pngAnti-PCTAIRE1/CDK16 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-CDK16 antibody (A06102-1). <br>Overlay histogram showing U20S cells stained with A06102-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK16 Antibody (A06102-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06102-1-cdk16-primary-antibodies-fc-testing-4.pngAnti-PCTAIRE1/CDK16 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-CDK16 antibody (A06102-1). <br>Overlay histogram showing 293T cells stained with A06102-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK16 Antibody (A06102-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-b2-ccnb2-picoband-trade-antibody-a02040-1-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02040-1-ccnb2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin B2/CCNB2 Antibody Picoband® Western blot analysis of CCNB2 using anti-CCNB2 antibody (A02040-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: rat testicular tissue lysates, <br>
Lane 4: mouse testicular tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB2 antigen affinity purified polyclonal antibody (Catalog # A02040-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB2 at approximately 55KD. The expected band size for CCNB2 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02040-1-ccnb2-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin B2/CCNB2 Antibody Picoband® IHC analysis of CCNB2 using anti-CCNB2 antibody (A02040-1). <br>
CCNB2 was detected in paraffin-embedded section of human Ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB2 Antibody (A02040-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02040-1-ccnb2-primary-antibodies-ihc-testing-3.jpgAnti-Cyclin B2/CCNB2 Antibody Picoband® IHC analysis of CCNB2 using anti-CCNB2 antibody (A02040-1). <br>
CCNB2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB2 Antibody (A02040-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02040-1-ccnb2-primary-antibodies-fc-testing-4.pngAnti-Cyclin B2/CCNB2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-CCNB2 antibody (A02040-1). <br>Overlay histogram showing A431 cells stained with A02040-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB2 Antibody (A02040-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02040-1-ccnb2-primary-antibodies-if-testing-5.jpgAnti-Cyclin B2/CCNB2 Antibody Picoband® IF analysis of CCNB2 using anti-CCNB2 antibody (A02040-1). <br>
CCNB2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCNB2 Antibody (A02040-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-axl-picoband-trade-antibody-a00226-2-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00226-2-axl-primary-antibodies-wb-testing-1.jpgAnti-AXL Antibody Picoband® Western blot analysis of AXL using anti-AXL antibody (A00226-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A431 whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXL antigen affinity purified polyclonal antibody (Catalog # A00226-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AXL at approximately 140KD. The expected band size for AXL is at 98KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00226-2-axl-primary-antibodies-fc-testing-2.pngAnti-AXL Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-AXL antibody (A00226-2). <br>Overlay histogram showing HeLa cells stained with A00226-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AXL Antibody (A00226-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-atp11c-picoband-trade-antibody-a06516-1-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06516-1-atp11c-primary-antibodies-wb-testing-1_1.jpgAnti-ATP11C Antibody Picoband® Western blot analysis of ATP11C using anti-ATP11C antibody (A06516-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: human A549 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP11C antigen affinity purified polyclonal antibody (Catalog # A06516-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP11C at approximately 129 kDa. The expected band size for ATP11C is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06516-1-atp11c-primary-antibodies-icc-testing-2.jpgAnti-ATP11C Antibody Picoband® IF analysis of ATP11C using anti-ATP11C antibody (A06516-1). <br>
ATP11C was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ATP11C Antibody (A06516-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06516-1-atp11c-primary-antibodies-fc-testing-3.pngAnti-ATP11C Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ATP11C antibody (A06516-1). <br>Overlay histogram showing A549 cells stained with A06516-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP11C Antibody (A06516-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-asxl1-picoband-trade-antibody-a01099-1-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01099-1-asxl1-primary-antibodies-wb-testing-1.jpgAnti-ASXL1 Antibody Picoband® Western blot analysis of ASXL1 using anti-ASXL1 antibody (A01099-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human COLO-32 whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASXL1 antigen affinity purified polyclonal antibody (Catalog # A01099-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASXL1 at approximately 165KD. The expected band size for ASXL1 is at 165KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01099-1-asxl1-primary-antibodies-fc-testing-2.pngAnti-ASXL1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ASXL1 antibody (A01099-1). <br>Overlay histogram showing SiHa cells stained with A01099-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASXL1 Antibody (A01099-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-accn1-asic2-picoband-trade-antibody-a04055-1-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04055-1-asic2-primary-antibodies-wb-testing-1.jpgAnti-ACCN1/ASIC2 Antibody Picoband® Western blot analysis of ASIC2 using anti-ASIC2 antibody (A04055-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human U2OS whole cell lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat liver tissue lysates, <br>
Lane 7: rat ovarian tissue lysates, <br>
Lane 8: mouse brain tissue lysates, <br>
Lane 9: mouse liver tissue lysates, <br>
Lane 10: mouse ovarian tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC2 antigen affinity purified polyclonal antibody (Catalog # A04055-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC2 at approximately 58KD. The expected band size for ASIC2 is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04055-1-asic2-primary-antibodies-ihc-testing-1.jpgAnti-ACCN1/ASIC2 Antibody Picoband®IHC analysis of ASIC2 using anti-ASIC2 antibody (A04055-1). <br>ASIC2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ASIC2 Antibody (A04055-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04055-1-asic2-primary-antibodies-icc-testing-2.jpgAnti-ACCN1/ASIC2 Antibody Picoband® IF analysis of ASIC2 using anti-ASIC2 antibody (A04055-1). <br>
ASIC2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ASIC2 Antibody (A04055-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04055-1-asic2-primary-antibodies-fc-testing-3.pngAnti-ACCN1/ASIC2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ASIC2 antibody (A04055-1). <br>Overlay histogram showing A549 cells stained with A04055-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04055-1-asic2-primary-antibodies-fc-testing-4.pngAnti-ACCN1/ASIC2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-ASIC2 antibody (A04055-1). <br>Overlay histogram showing PC-3 cells stained with A04055-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC2 Antibody (A04055-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-angiopoietin-2-angpt2-picoband-trade-antibody-a00370-2-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-wb-testing-1.jpgAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human SW620 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: mouse spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 66KD. The expected band size for ANGPT2 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-icc-testing-2.jpgAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® IF analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). <br>
ANGPT2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ANGPT2 Antibody (A00370-2) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-fc-testing-3.pngAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-ANGPT2 antibody (A00370-2). <br>Overlay histogram showing HeLa cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-fc-testing-4.pngAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ANGPT2 antibody (A00370-2). <br>Overlay histogram showing U937 cells stained with A00370-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody (A00370-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-ihc-testing-5.jpgAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). <br>
ANGPT2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANGPT2 Antibody (A00370-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-ihc-testing-6.jpgAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). <br>
ANGPT2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANGPT2 Antibody (A00370-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00370-2-angpt2-primary-antibodies-ihc-testing-7.jpgAnti-Angiopoietin-2/ANGPT2 Antibody Picoband® IHC analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). <br>
ANGPT2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANGPT2 Antibody (A00370-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-p5cs-aldh18a1-picoband-trade-antibody-a06119-2-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06119-2-aldh18a1-primary-antibodies-wb-testing-1.jpgAnti-P5CS/ALDH18A1 Antibody Picoband® Western blot analysis of ALDH18A1 using anti-ALDH18A1 antibody (A06119-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human MDA-MB-453 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: rat heart tissue lysates, <br>
Lane 6: mouse skeletal muscle tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH18A1 antigen affinity purified polyclonal antibody (Catalog # A06119-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH18A1 at approximately 87KD. The expected band size for ALDH18A1 is at 87KD.
https://www.bosterbio.com/products/primary-antibodies/anti-aryl-hydrocarbon-receptor-ahr-picoband-trade-antibody-a00225-3-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-3-ahr-primary-antibodies-wb-testing-1.jpgAnti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband® Western blot analysis of Ahr using anti-Ahr antibody (A00225-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat PC-12 whole cell lysates, <br>
Lane 3: rat C6 whole cell lysates, <br>
Lane 4: rat RH35 whole cell lysates, <br>
Lane 5: mouse liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ahr antigen affinity purified polyclonal antibody (Catalog # A00225-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ahr at approximately 110KD. The expected band size for Ahr is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-3-ahr-primary-antibodies-fc-testing-2.pngAnti-Aryl hydrocarbon Receptor/Ahr Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-Ahr antibody (A00225-3). <br>Overlay histogram showing Neuro-2a cells stained with A00225-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ahr Antibody (A00225-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-adam28-picoband-trade-antibody-a06873-2-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06873-2-adam28-primary-antibodies-wb-testing-1.jpgAnti-Adam28 Antibody Picoband® Western blot analysis of Adam28 using anti-Adam28 antibody (A06873-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adam28 antigen affinity purified polyclonal antibody (Catalog # A06873-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adam28 at approximately 87KD. The expected band size for Adam28 is at 87KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06873-2-adam28-primary-antibodies-ihc-testing-2.jpgAnti-Adam28 Antibody Picoband® IHC analysis of Adam28 using anti-Adam28 antibody (A06873-2). <br>
Adam28 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adam28 Antibody (A06873-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06873-2-adam28-primary-antibodies-ihc-testing-3.jpgAnti-Adam28 Antibody Picoband® IHC analysis of Adam28 using anti-Adam28 antibody (A06873-2). <br>
Adam28 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adam28 Antibody (A06873-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06873-2-adam28-primary-antibodies-ihc-testing-4.jpgAnti-Adam28 Antibody Picoband® IHC analysis of Adam28 using anti-Adam28 antibody (A06873-2). <br>
Adam28 was detected in paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adam28 Antibody (A06873-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06873-2-adam28-primary-antibodies-fc-testing-5.pngAnti-Adam28 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Adam28 antibody (A06873-2). <br>Overlay histogram showing HEPA1-6 cells stained with A06873-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Adam28 Antibody (A06873-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rpoa-antibody-dz33981-boster.html2026-03-24T05:26:01+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/anti-adam15-picoband-trade-antibody-a02593-4-boster.html2026-03-24T05:26:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02593-4-adam15-primary-antibodies-wb-testing-1.jpgAnti-ADAM15 Antibody Picoband® Western blot analysis of ADAM15 using anti-ADAM15 antibody (A02593-4).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human MDA-MB-453 whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM15 antigen affinity purified polyclonal antibody (Catalog # A02593-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM15 at approximately 100KD. The expected band size for ADAM15 is at 93KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02593-4-adam15-primary-antibodies-fc-testing-2.pngAnti-ADAM15 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-ADAM15 antibody (A02593-4). <br>Overlay histogram showing A431 cells stained with A02593-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM15 Antibody (A02593-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-cas-antibody-dz33927-1-boster.html2026-04-03T05:00:57+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-sich211-251b21-1-grikl-antibody-dz33936-1-boster.html2026-03-24T05:26:01+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/anti-scs2-antibody-dz33979-boster.html2026-03-24T05:26:01+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-clipa7-antibody-dz33987-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/anti-clipb17-antibody-dz33988-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/polyclonal-anti-clipb14-antibody-dz33986-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-spclip1-antibody-dz33985-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-me3eb-antibody-dz33938-1-boster.html2026-03-24T05:26:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33938-1-me31b-custom-antibodies-wb-testing-1.jpgAnti-Fruit fly Me31B Antibody Western blot analysis of Me31B using anti-Fruit fly Me31B antibody (DZ33938-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with non-fat dry milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fruit fly Me31B antibody (DZ33938-1) at 1:10,000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000, incubation 1h at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Me31B at approximately 52 kDa. The expected band size for Me31B is at 52 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-cg17352-target-2-culd-antibody-dz33940-2-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-eys-antibody-dz06950-1-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-mitf-antibody-dz00269-1-boster.html2026-04-04T05:00:56+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-hp1488-antibody-dz33983-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-motb-antibody-dz33982-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-vcam1b-antibody-dz01199-1-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-pgk3-antibody-dz33956-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-musashi-1-msi1-antibody-monoclonal-2b9-m05052-1-boster.html2026-03-24T05:26:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05052-1-msi-primary-antibodies-wb-testing-1_1.jpgAnti-Musashi 1/Msi1 Antibody Picoband® (monoclonal, 2B9) Western blot analysis of MSI using anti-MSI antibody (M05052-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 tissue lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MSI antigen affinity purified polyclonal antibody (Catalog # M05052-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05052-1-msi-primary-antibodies-ihc-testing-2.jpgAnti-Musashi 1/Msi1 Antibody Picoband® (monoclonal, 2B9) IHC analysis of MSI using anti-MSI antibody (M05052-1). <br>MSI was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1g/ml mouse anti-MSI Antibody (M05052-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05052-1-msi-primary-antibodies-ihc-testing-3.jpgAnti-Musashi 1/Msi1 Antibody Picoband® (monoclonal, 2B9)Figure 3 IHC analysis of MSI using anti-MSI antibody (M05052-1). <br>MSI was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1g/ml mouse anti-MSI Antibody (M05052-1) overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05052-1-msi-primary-antibodies-if-testing-4.jpgAnti-Musashi 1/Msi1 Antibody Picoband® (monoclonal, 2B9) IF analysis of MSI using anti-MSI antibody (M05052-1). <br>
MSI was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MSI Antibody (M05052-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-crocc2-antibody-dz41006-boster.html2026-03-24T05:26:02+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfap-antibody-picoband-monoclonal-3f2-m00213-8-boster.html2026-03-24T05:26:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-wb-testing-1.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2) Western blot analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (Catalog # M00213-8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50KD. The expected band size for GFAP is at 50KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-ihc-testing-2.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2) IHC analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
GFAP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-ihc-testing-3.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2) IHC analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
GFAP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-ihc-testing-4.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)IHC analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
GFAP was detected in a paraffin-embedded section of rat cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-ihc-testing-5.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)IHC analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
GFAP was detected in a paraffin-embedded section of rat hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-ihc-testing-6.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)IHC analysis of GFAP using anti-GFAP antibody (M00213-8). <br>
GFAP was detected in a paraffin-embedded section of mouse hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-GFAP Antibody (M00213-8) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-gfap-primary-antibodies-if-testing-4.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2) IF analysis of Histone H3 and GFAP using anti-Histone H3 antibody (A12477-2) and anti-GFAP antibody (M00213-8). <br>
Histone H3 and GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Histone H3 antibody (A12477-2) and mouse anti-GFAP antibody (M00213-8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127), Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-fphar-16-1554945-g011.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)TUDCA promoted neuron regeneration along endogenous NSCs migration at day 7 after SCI. (A) Co-immunofluorescence showed endogenous NSCs (Nestin, green) and reactive astrocytes (GFAP, red) at the margin of the lesion site at day 7 after SCI. (B) Endogenous NSCs (Nestin, green) and neuron (NeuN, red) at the margin of the lesion site at day 7 after SCI. (C, D) Quantitative polymerase chain reaction (qPCR) showing the expression of Nestin, GFAP, NeuN, MAP2 and Oligo 2 at day 7 after SCI. All experiments were performed in triplicated and data were presented means ± SEM, n = 3 per group. *P < 0.05, **P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-fphar-16-1554945-g009.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)TUDCA regulated macrophages and reactive astrocytes distribution. Co-immunofluorescence images showed macrophages (CD68, red) and reactive astrocytes (GFAP, green) at day 3 and day 7 after SCI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-8-fphar-16-1554945-g007.jpgAnti-GFAP Antibody Picoband® (monoclonal, 3F2)TUDCA regulated monocytes distribution and impacted glial scar formation. Co-immunofluorescence images showed reactive astrocytes (GFAP, green) and monocytes (CD11b, red) at day 3 and day 7 after SCI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp5h-picoband-trade-antibody-monoclonal-m09565-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09565-atp5h-primary-antibodies-wb-testing-1_1.jpgAnti-ATP5H in Antibody Picoband® (monoclonal, 6B12)Western blot analysis of ATP5H using anti-ATP5H antibody (M09565).
<br>
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human 293T whole cell lysates.<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat liver tissue lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse liver tissue lysates.<br>
After Electrophoresis, proteins were tATP5Hsferred to a Nitrocellulose membATP5He at 150mA for 50-90 minutes. Blocked the membATP5He with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membATP5He was incubated with mouse anti-ATP5H antigen affinity purified monoclonal antibody (Catalog # M09565) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 kDa. The expected band size for ATP5H is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09565-atp5h-primary-antibodies-ihc-testing-2_1.jpgAnti-ATP5H in Antibody Picoband® (monoclonal, 6B12) IHC analysis of ATP5H using anti-ATP5H antibody (M09565). <br>
ATP5H was detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09565-atp5h-primary-antibodies-ihc-testing-3_1.jpgAnti-ATP5H in Antibody Picoband® (monoclonal, 6B12) IHC analysis of ATP5H using anti-ATP5H antibody (M09565). <br>
ATP5H was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09565-atp5h-primary-antibodies-if-testing-4_1.jpgAnti-ATP5H in Antibody Picoband® (monoclonal, 6B12) IF analysis of ATP5H using anti-ATP5H antibody (M09565). <br>
ATP5H was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ATP5H Antibody (M09565) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synaptophys-picoband-trade-antibody-monoclonal-m05049-3-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04526-syp-primary-antibodies-wb-testing-1.jpgAnti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) Western blot analysis of SYP using anti-SYP antibody (M05049-3). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat brain whole cell lysates, <br>Lane 3: mouse brain whole cell lysates, <br>Lane 4: mouse brain whole cell lysates, <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody (Catalog # M05049-3) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab11b-picoband-trade-antibody-monoclonal-m04526-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04526-rab11b-primary-antibodies-wb-testing-1_1.jpgAnti-RAB11B Antibody Picoband® (monoclonal, 6C5) Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human placenta whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates. <br>
Lane 6: human Caco-2 whole cell lysates. <br>
Lane 7: human THP-1 whole cell lysates. <br>
Lane 8: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # M04526) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04526-rab11b-primary-antibodies-wb-testing-2.jpgAnti-RAB11B Antibody Picoband® (monoclonal, 6C5) Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat brain tissue lysates, <br>Lane 2: rat lung whole cell lysates, <br>Lane 3: rat spleen whole cell lysates, <br>Lane 4: rat C6 whole cell lysates, <br>Lane 5: mouse brain whole cell lysates. <br>Lane 6: mouse lung whole cell lysates. <br>Lane 7: mouse spleen whole cell lysates. <br>Lane 8: mouse Neuro-2a whole cell lysates. <br>Lane 9: mouse RAW246.7 whole cell lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # M04526) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04526-rab11b-primary-antibodies-fc-testing-3_1.pngAnti-RAB11B Antibody Picoband® (monoclonal, 6C5) Flow Cytometry analysis of Caco-2 cells using anti-RAB11B antibody (M04526). <br>Overlay histogram showing Caco-2 cells stained with M04526 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB11B Antibody (M04526, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04526-rab11b-primary-antibodies-if-testing-4.jpgAnti-RAB11B Antibody Picoband® (monoclonal, 6C5) IF analysis of RAB11B using anti-RAB11B antibody (M04526). <br>
RAB11B was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RAB11B Antibody (M04526) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmg4-picoband-trade-antibody-monoclonal-m02834-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-hmgb3-primary-antibodies-wb-testing-1_1.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 7G13) Western blot analysis of HMGB3 using anti-HMGB3 antibody (M02834). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human T-47D whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human Caco-2 whole cell lysates. <br>
Lane 7: human SW620 whole cell lysates. <br>
Lane 8: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HMGB3 antigen affinity purified polyclonal antibody (Catalog # M02834) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-hmgb3-primary-antibodies-ihc-testing-2_1.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 7G13) IHC analysis of HMGB3 using anti-HMGB3 antibody (M02834). <br>
HMGB3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HMGB3 Antibody (M02834) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-hmgb3-primary-antibodies-if-testing-3_1.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 7G13) IF analysis of HMGB3 using anti-HMGB3 antibody (M02834). <br>
HMGB3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-HMGB3 Antibody (M02834) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-hmgb3-primary-antibodies-fc-testing-4_1.pngAnti-HMG4 Antibody Picoband® (monoclonal, 7G13) Flow Cytometry analysis of Hela cells using anti-HMGB3 antibody M02834). <br>Overlay histogram showing Hela cells stained with M02834 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HMGB3 Antibody (M02834, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmg4-picoband-trade-antibody-monoclonal-m02834-1-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-1-hmgb3-primary-antibodies-wb-testing-1.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 8H9) Western blot analysis of HMGB3 using anti-HMGB3 antibody (M02834-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human CACO-2 whole cell lysates, <br>
Lane 4: human 293T whole cell lysates, <br>
Lane 5: human THP-1 whole cell lysates, <br>
Lane 6: human HepG2 whole cell lysates, <br>
Lane 7: human MCF-7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HMGB3 antigen affinity purified monoclonal antibody (Catalog # M02834-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HMGB3 at approximately 23 kDa. The expected band size for HMGB3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-1-hmgb3-primary-antibodies-wb-testing-2.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 8H9) Western blot analysis of HMGB3 using anti-HMGB3 antibody (M02834-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse kidney tissue lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse lung tissue lysates, <br>
Lane 6: mouse ovary tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HMGB3 antigen affinity purified monoclonal antibody (Catalog # M02834-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HMGB3 at approximately 23 kDa. The expected band size for HMGB3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-1-hmgb3-primary-antibodies-if-testing-3_1.jpgAnti-HMG4 Antibody Picoband® (monoclonal, 8H9) IF analysis of HMGB3 using anti-HMGB3 antibody (M02834-1). <br>
HMGB3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-HMGB3 Antibody (M02834-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-1-hmgb3-primary-antibodies-fcm-testing-4.pngAnti-HMG4 Antibody Picoband® (monoclonal, 8H9) Flow Cytometry analysis of Hela cells using anti-HMGB3 antibody (M02834-1). <br>Overlay histogram showing Hela cells stained with M02834-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HMGB3 Antibody (M02834-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mitofusin-1-picoband-trade-antibody-monoclonal-m02172-1-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02172-1-mfn1-primary-antibodies-wb-testing-1.jpgAnti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) Western blot analysis of MFN1 using anti-MFN1 antibody (M02172-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela tissue lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MFN1antigen affinity purified polyclonal antibody (Catalog # M02172-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MFN1 at approximately 84KD. The expected band size for MFN1 is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02172-1-mfn1-primary-antibodies-if-testing-2.jpgAnti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3) IF analysis of MFN1 using anti-MFN1 antibody (M02172-1). <br>
MFN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MFN1 Antibody (M02172-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prdx6-picoband-trade-antibody-monoclonal-m01847-1-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01847-1-prdx6-primary-antibodies-wb-testing-1_1.jpgAnti-PRDX6 Antibody Picoband® (monoclonal, 6I8) Western blot analysis of PRDX6 using anti-PRDX6 antibody (M01847-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela tissue lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human SW620 whole cell lysates. <br>
Lane 6: rat RH35 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PRDX6 ntigen affinity purified polyclonal antibody (Catalog # M01847-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 29KD.The expected band size for PRDX6 is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01847-1-prdx6-primary-antibodies-if-testing-2_1.jpgAnti-PRDX6 Antibody Picoband® (monoclonal, 6I8) IF analysis of PRDX6 using anti-PRDX6 antibody (M01847-1). <br>
PRDX6 was detected in immunocytochemical section of A549. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PRDX6 Antibody (M01847-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01847-1-prdx6-primary-antibodies-fc-testing-3_1.pngAnti-PRDX6 Antibody Picoband® (monoclonal, 6I8) Flow Cytometry analysis of Hela cells using anti-PRDX6 antibody (M01847-1). <br>Overlay histogram showing Hela cells stained with M01847-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PRDX6 Antibody (M01847-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01847-1-prdx6-primary-antibodies-ihc-testing-4.jpgAnti-PRDX6 Antibody Picoband® (monoclonal, 6I8) IHC analysis of PRDX6 using anti-PRDX6 antibody (M01847-1). <br>
PRDX6 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PRDX6 Antibody (M01847-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gaa-picoband-trade-antibody-monoclonal-m01548-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-wb-testing-1_1.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) Western blot analysis of GAA using anti-GAAantibody (M01548). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 tissue lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAA antigen affinity purified polyclonal antibody (Catalog # M01548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-ihc-testing-2_1.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) IHC analysis of GAA using anti-GAA antibody (M01548). <br>
GAA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GAA Antibody (M01548) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-ihc-testing-3_1.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) IHC analysis of GAA using anti-GAA antibody (M01548). <br>
GAA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GAA Antibody (M01548) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-ihc-testing-4_1.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) IHC analysis of GAA using anti-GAA antibody (M01548). <br>
GAA was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-GAA Antibody (M01548) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-if-testing-5_2.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) IF analysis of GAA using anti-GAA antibody (M01548). <br>
GAA was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-GAA Antibody (M01548) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-gaa-primary-antibodies-if-testing-6_2.jpgAnti-GAA Antibody Picoband® (monoclonal, 2G7) IF analysis of GAA using anti-GAA antibody (M01548). <br>
GAA was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-GAA Antibody (M01548) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-idh1-isocitrate-dehydrogenase-antibody-m00129-1-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-1-idh1-primary-antibodies-wb-testing-1.jpgAnti-IDH1 Antibody Picoband® (monoclonal, 16H7) Western blot analysis of IDH1 using anti-IDH1 antibody (M00129-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 tissue lysates, <br>
Lane 2: human Caco-2 whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: human THP-1 whole cell lysates, <br>
Lane 5: human Hela whole cell lysates. <br>
Lane 6: human K562 whole cell lysates. <br>
Lane 7: human PC-3 whole cell lysates. <br>
Lane 8: human HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IDH1antigen affinity purified polyclonal antibody (Catalog # M00129-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-1-idh1-primary-antibodies-wb-testing-2_1.jpgAnti-IDH1 Antibody Picoband® (monoclonal, 16H7)
Western blot analysis of IDH1 using anti-IDH1 antibody (M00129-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat RH35 whole cell lysates, <br>
Lane 3: mouse liver whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IDH1antigen affinity purified polyclonal antibody (Catalog # M00129-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-1-idh-primary-antibodies-if-testing-3_1.jpgAnti-IDH1 Antibody Picoband® (monoclonal, 16H7)
IF analysis of IDH1 using anti-IDH1 antibody (M00129-1). <br>
IDH1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-IDH1 Antibody (M00129-1) overnight at 4℃. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37℃. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-1-idh1-primary-antibodies-fcm-testing-4_1.pngAnti-IDH1 Antibody Picoband® (monoclonal, 16H7) Flow Cytometry analysis of CACO-2 cells using anti-IDH1 antibody (M00129-1). <br>Overlay histogram showing CACO-2 cells stained with M00129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IDH1 Antibody (M00129-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-bnaa07g20720d-antibody-dz33980-boster.html2026-03-24T05:26:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33980-bnaa07g20720d-primary-antibodies-wb-testing-1.jpgPolyclonal Anti-BnaA07g20720D Antibody Western blot analysis of BnaA07g20720D using anti-BnaA07g20720D antibody (DZ33980). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BnaA07g20720D antigen affinity purified polyclonal antibody (Catalog # DZ33980) at 1:5000 for 90 min at room temperature, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 90 min at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-cg17352-antibody-dz33940-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-mef2-antibody-dz01398-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01398-1-mef2-primary-antibodies-if-testing-1.jpgAnti-Zebrafish Mef2 Antibody Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4°C overnight). AlexaFluo546 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01398-1-41467_2025_59169_fig7_html.pngAnti-Zebrafish Mef2 Antibody Mmp14b is a regulator of CM protrusion. A Collagen hybridizing peptide (CHP) and phalloidin staining of a wild-type ventricle at 10 dpci. Yellow box denotes the area in the zoomed image. B In situ hybridization of mmp14b expression in a wild-type ventricle at 7 dpci. Black dashed line denotes the approximate injury border. C Colocalization of mmp14b (HCR, magenta) with CMs (MHC immunostaining), endocardial cells (vwf HCR), macrophages (GFP immunostaining in Tg(mpeg1:EGFP) ventricles, and fibroblasts ( col12a1a (HCR, green) and postnb (HCR, cyan) in the cortical BZ region at 10 dpci. Yellow box in the schematic of the heart marks the cortical CM region depicted in the zoomed images. Created in BioRender. Beisaw, A. (2025) . D Schematic depicting the exon structure of the mmp14b locus and CRISPR/Cas9-induced full-length deletion between exons 2 and 9 of mmp14b . Created in BioRender. Beisaw, A. (2025) . E Mmp14b wild-type and putative mutant protein domain structure (left). RT-PCR of the mmp14b open reading frame from wild-type and mmp14b Δ/Δ mutant embryos (right). SP signal peptide, Pro propeptide, Cat catalytic domain, H hinge region, TM transmembrane domain, C C-terminal tail. F RT-qPCR of mmp14b and mmp14a expression in single ventricles from mmp14b Δ/Δ ( n = 5 ventricles) and wild-type siblings ( n = 5 ventricles) at 10 dpci. Data are presented as mean ± SD. P -values were calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. G Phalloidin staining of thick cryosections from mmp14b Δ/Δ and wild-type sibling ventricles at 10 dpci (left). Quantification of CM protrusion length (right, mmp14b Δ/Δ n = 1124 CM protrusions from 8 ventricles, wild-type sibling n = 1480 CM protrusions from 9 ventricles). Data are presented as violin plots of all points with solid gray lines indicating the median and dotted gray lines indicating 25th and 75th percentile. P -values were calculated using a two-sided Mann–Whitney test. Source data are presented in the Source Data file. H Picrosirius red staining of collagen in mmp14b Δ/Δ ( n = 8 ventricles) and wild-type sibling ( n = 10 ventricles) at 60 dpci (left). Quantification of scar area (% of ventricle area) on the right. Data are presented as mean ± SD. P -value was calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. I Quantification of CM proliferation within 100 μm of the wound border from PCNA/Mef2 immunostaining in mmp14b Δ/Δ ( n = 3 ventricles) and wild-type sibling ( n = 4 ventricles) at 7 dpci. Data are presented as mean ± SD. P -value was calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. Scale bars: 100 μm in ( A , B , G , and H ), 20 μm in zoomed image in ( A and C ). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59169-4'>40268967</a></b>
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-escargot-antibody-dz33977-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-prospero-antibody-dz33976-boster.html2026-03-24T05:26:04+00:00daily0.9
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https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-hic2-antibody-dz10161-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-syt4-antibody-dz10762-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-f7-antibody-dz00693-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-vwf-antibody-dz00270-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-protein-g-ba1121-boster.html2026-03-24T05:26:04+00:00daily0.9
https://www.bosterbio.com/products/all-secondary-antibodies/peroxidase-conjugated-protein-g-ba1083-boster.html2026-03-24T05:26:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_10_1.pngHRP Conjugated Protein GBoster Kit Box
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-emb140-antibody-dz33967-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-rho1-antibody-dz33978-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-ty1-prime-p18-antibody-dz33974-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-ty1-prime-p18-antibody-dz33969-1-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-sgs4-antibody-dz33966-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-tbph-antibody-dz33965-boster.html2026-04-03T05:00:57+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-pnr-antibody-dz33964-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-so-antibody-dz33963-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-toy-antibody-dz33962-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-ey-antibody-dz33961-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-lili-antibody-dz33960-boster.html2026-03-24T05:26:05+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cavin1a-antibody-dz33959-boster.html2026-03-24T05:26:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33959-cavin1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cavin1a Antibody Western blot analysis of Cavin1a using anti-Cavin1a antibody (DZ33959). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Zebrafish, <br>
Lane 2: Zebrafish head tissue lysates, <br>
Lane 3: Zebrafish body tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cavin1a antigen affinity purified polyclonal antibody (DZ33959) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cavin1a at approximately 47 kDa. The expected band size for Cavin1a is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33959-cavin1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cavin1a Antibody IHC analysis of Cavin1a using anti-Cavin1a antibody (DZ33959). <br>
Cavin1a was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1a Antibody (DZ33959) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33959-cavin1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Cavin1a Antibody IHC analysis of Cavin1a using anti-Cavin1a antibody (DZ33959). <br>
Cavin1a was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1a Antibody (DZ33959) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33959-cavin1a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Cavin1a AntibodyIHC analysis of Cavin1a using anti-Cavin1a antibody (DZ33959). <br>
Cavin1a was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1a Antibody (DZ33959) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-rab18a-antibody-dz33952-boster.html2026-03-24T05:26:06+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-egg-antibody-dz33951-boster.html2026-03-24T05:26:06+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-su-var-3-9-antibody-dz33950-boster.html2026-03-24T05:26:06+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cavin4b-antibody-dz33949-boster.html2026-03-24T05:26:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33949-cavin4b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Cavin4b Antibody IHC analysis of Cavin4b using anti-Cavin4b antibody (DZ33949). <br>
Cavin4b was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin4b Antibody (DZ33949) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cavin4a-antibody-dz33948-boster.html2026-03-24T05:26:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33948-cavin4a-primary-antibodies-ihc-testing-1_1.jpgAnti-Zebrafish Cavin4a Antibody IHC analysis of Cavin4a using anti-Cavin4a antibody (DZ33948). <br>
Cavin4a was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin4a Antibody (DZ33948) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33948-cavin4a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cavin4a Antibody IHC analysis of Cavin4a using anti-Cavin4a antibody (DZ33948). <br>
Cavin4a was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin4a Antibody (DZ33948) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-cimex-antibody-dz33934-boster.html2026-03-24T05:26:06+00:00daily0.9
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https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-shg1-antibody-dz33925-boster.html2026-03-24T05:26:07+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-synelb-201-antibody-dz33924-boster.html2026-03-24T05:26:07+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-anterior-gradient-portein-2-antibody-dz33923-boster.html2026-03-24T05:26:07+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-otofa-otoferlin-antibody-dz33922-boster.html2026-03-24T05:26:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33922-otofa-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Otofa/Otoferlin Antibody IHC analysis of Otofa/Otoferlin using anti-Otofa/Otoferlin antibody (DZ33922). <br>
Otofa/Otoferlin was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Otofa/Otoferlin1 Antibody (DZ33922) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/polyclonal-anti-cg6353-antibody-dz33921-1-boster.html2026-03-24T05:26:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33921-1-cg6353-primary-antibodies-if-testing-1.pngAnti-Fruit fly CG6353 Antibody IF analysis of CG6353 using anti-CG6353 antibody (DZ33921-1). <br>
CG6353 was detected in a paraffin-embedded section of drosophila larvae tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 4% horse serum. The tissue section was then incubated with rabbit anti-CG6353 Antibody (DZ33921-1) at 1:100 dilution overnight at 4°C. Anti-Rb-A488 was used as secondary antibody at 1:1000 dilution. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-fer1l6-antibody-dz18905-boster.html2026-03-24T05:26:07+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-wfikkn1-antibody-dz11633-boster.html2026-03-24T05:26:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz11633-wfikkn1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish wfikkn1 Antibody Western blot analysis of Wfikkn1 using anti-Wfikkn1 antibody (DZ11633). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: Zebrafish, <br>
Lane 2: Zebrafish head tissue lysates, <br>
Lane 3: Zebrafish body tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wfikkn1 antigen affinity purified polyclonal antibody (DZ11633) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wfikkn1 at approximately 62 kDa. The expected band size for Wfikkn1 is at 62 kDa.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-abcc12-antibody-dz10852-boster.html2026-03-24T05:26:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz10852-abcc12-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ABCC12 Antibody IHC analysis of ABCC12 using anti-ABCC12 antibody (DZ10852). <br>
ABCC12 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCC12 Antibody (DZ10852) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz10852-abcc12-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ABCC12 Antibody IHC analysis of ABCC12 using anti-ABCC12 antibody (DZ10852). <br>
ABCC12 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCC12 Antibody (DZ10852) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz10852-abcc12-primary-antibodies-if-testing-1.jpgAnti-Zebrafish ABCC12 Antibody IF analysis of ABCC12 using anti-ABCC12 antibody (DZ10852). <br>
ABCC12 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ABCC12 Antibody (DZ10852) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-pxdn-antibody-dz06456-boster.html2026-03-24T05:26:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz06456-pxdn-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PXDN Antibody IHC analysis of PXDN using anti-PXDN antibody (DZ06456). <br>
PXDN was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PXDN Antibody (DZ06456) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-nap1l4a-antibody-dz08739-boster.html2026-03-24T05:26:07+00:00daily0.9
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https://www.bosterbio.com/products/all-recombinant-proteins/zero-dollar-test-product-zerotest-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/r/prota1l190-1-test.jpgAPI testadd2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/M/0/M02393-2-HSP90B1-ICC-test-1-test.pngAPI testadd2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/t/e/test-1.jpgAPI test<h4> Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T308) AKT1 Antibody (A00024T308-1).</h4>
Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue<br>
For more protocol information of IHChttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/t/e/test-1_1.jpgAPI test<h4> Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T308) AKT1 Antibody (A00024T308-1).</h4>
Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue<br>
For more protocol information of IHChttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/t/e/test-2.jpgAPI test<h4> Immunohistochemistry validation of AKT1 using Anti-AKT (phospho-T308) AKT1 Antibody (A00024T308-1).</h4>
Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue<br>
For more protocol information of IHChttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/t/e/test-3.jpgAPI testImmunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC Immunohistochemistry (IHC) analysis of p-AKT(T308) pAb in paraffin-embedded human breast carcinoma tissue For more protocol information of IHC
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https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cavin1b-antibody-dz02695-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02695-cavin1b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Cavin1b Antibody IHC analysis of Cavin1b using anti-Cavin1b antibody (DZ02695). <br>
Cavin1b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1b Antibody (DZ02695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02695-cavin1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cavin1b Antibody IHC analysis of Cavin1b using anti-Cavin1b antibody (DZ02695). <br>
Cavin1b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1b Antibody (DZ02695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02695-cavin1b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Cavin1b AntibodyIHC analysis of Cavin1b using anti-Cavin1b antibody (DZ02695). <br>
Cavin1b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1b Antibody (DZ02695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02695-cavin1b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Cavin1b AntibodyIHC analysis of Cavin1b using anti-Cavin1b antibody (DZ02695). <br>
Cavin1b was detected in a paraffin-embedded section of zebrafish intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1b Antibody (DZ02695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz02695-cavin1b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish Cavin1b AntibodyIHC analysis of Cavin1b using anti-Cavin1b antibody (DZ02695). <br>
Cavin1b was detected in a paraffin-embedded section of zebrafish pancrease tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cavin1b Antibody (DZ02695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-abcb11-antibody-dz01657-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01657-abcb11-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ABCB11 Antibody IHC analysis of ABCB11 using anti-ABCB11 antibody (DZ01657). <br>
ABCB11 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB11 Antibody (DZ01657) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01657-abcb11-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ABCB11 AntibodyIHC analysis of ABCB11 using anti-ABCB11 antibody (DZ01657). <br>
ABCB11 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB11 Antibody (DZ01657) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd46-picoband-trade-antibody-monoclonal-m00377-2-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00377-2-cd46-primary-antibodies-wb-testing-1.jpgAnti-CD46 Antibody Picoband® (monoclonal, 9E9) Western blot analysis of CD46 using anti ZO-1 antibody (M00377-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD46 antigen affinity purified polyclonal antibody (Catalog # M00377-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD46 at approximately 50-70KD. The expected band size for CD46 is at 44KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00377-2-cd46-primary-antibodies-ihc-testing-2.jpgAnti-CD46 Antibody Picoband® (monoclonal, 9E9)
IHC analysis of CD46 using anti-CD46 antibody (M00377-2). <br>
DCK was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00377-2-cd46-primary-antibodies-ihc-testing-3.jpgAnti-CD46 Antibody Picoband® (monoclonal, 9E9)
IHC analysis of CD46 using anti-CD46 antibody (M00377-2). <br>
DCK was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD46 Antibody (M00377-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00377-2-cd46-primary-antibodies-fc-testing-4.pngAnti-CD46 Antibody Picoband® (monoclonal, 9E9) Flow Cytometry analysis of PBMC cells using anti-CD46 antibody (M00377-2). <br>Overlay histogram showing PBMC cells stained with M00377-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD46 Antibody (M00377-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/catalog/product/view/id/556322026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_10_2.pngHRP Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L)Boster Kit Box
https://www.bosterbio.com/products/primary-antibodies/anti-agrin-agrn-picoband-trade-antibody-a04649-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04649-agrn-primary-antibodies-wb-testing-1.jpgAnti-Agrin/AGRN Picoband® Antibody Western blot analysis of AGRN using anti-AGRN antibody (A04649).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.<br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGRN antigen affinity purified polyclonal antibody (Catalog # A04649) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGRN at approximately 205-217KD. The expected band size for AGRN is at 217KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04649-agrn-primary-antibodies-ihc-testing-2.jpgAnti-Agrin/AGRN Picoband® Antibody IHC analysis of Agrin/AGRN using anti-Agrin/AGRN antibody (A04649). <br>
Agrin/AGRN was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Agrin/AGRN Antibody (A04649) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04649-agrn-primary-antibodies-if-testing-3.jpgAnti-Agrin/AGRN Picoband® Antibody IF analysis of Agrin/AGRN using anti-Agrin/AGRN antibody (A04649). <br>
Agrin/AGRN was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Agrin/AGRN Antibody (A04649) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04649-agrn-primary-antibodies-fcm-testing-4.pngAnti-Agrin/AGRN Picoband® Antibody Flow Cytometry analysis of A549 cells using anti-Agrin/AGRN antibody (A04649). <br>Overlay histogram showing A549 cells stained with A04649 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Agrin/AGRN Antibody (A04649, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-akr7a2-picoband-trade-antibody-a06949-1-boster.html2026-03-24T05:26:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-wb-testing-1_1.jpgAnti-AKR7A2 Picoband® Antibody Western blot analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: rat brain tissue lysates, <br>
Lane 4: rat kidney tissue lysates, <br>
Lane 5: mouse brain tissue lysates, <br>
Lane 6: mouse kidney tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR7A2 antigen affinity purified polyclonal antibody (Catalog # A06949-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR7A2 at approximately 36 kDa. The expected band size for AKR7A2 is at 35-40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-ihc-testing-2.jpgAnti-AKR7A2 Picoband® Antibody IHC analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). <br>
AKR7A2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR7A2 Antibody (A06949-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-ihc-testing-3.jpgAnti-AKR7A2 Picoband® Antibody IHC analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). <br>
AKR7A2 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR7A2 Antibody (A06949-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-ihc-testing-4.jpgAnti-AKR7A2 Picoband® Antibody IHC analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). <br>
AKR7A2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR7A2 Antibody (A06949-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-ihc-testing-5.jpgAnti-AKR7A2 Picoband® Antibody IHC analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). <br>
AKR7A2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR7A2 Antibody (A06949-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06949-1-akr7a2-primary-antibodies-fc-testing-6.pngAnti-AKR7A2 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-AKR7A2 antibody (A06949-1). <br>Overlay histogram showing THP-1 cells stained with A06949-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR7A2 Antibody (A06949-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd13-anpep-picoband-trade-antibody-a02591-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-2-anpep-primary-antibodies-wb-testing-1.jpgAnti-CD13/ANPEP Picoband® Antibody Western blot analysis of ANPEP using anti-ANPEP antibody (A02591-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANPEP antigen affinity purified polyclonal antibody (Catalog # A02591-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANPEP at approximately 150KD. The expected band size for ANPEP is at 150KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-2-anpep-primary-antibodies-ihc-testing-2.jpgAnti-CD13/ANPEP Picoband® Antibody IHC analysis of ANPEP using anti-ANPEP antibody (A02591-2). <br>
ANPEP was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANPEP Antibody (A02591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-2-anpep-primary-antibodies-ihc-testing-3.jpgAnti-CD13/ANPEP Picoband® Antibody IHC analysis of ANPEP using anti-ANPEP antibody (A02591-2). <br>
ANPEP was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANPEP Antibody (A02591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-2-anpep-primary-antibodies-ihc-testing-4.jpgAnti-CD13/ANPEP Picoband® Antibody IHC analysis of ANPEP using anti-ANPEP antibody (A02591-2). <br>
ANPEP was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ANPEP Antibody (A02591-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-2-anpep-primary-antibodies-fc-testing-5.pngAnti-CD13/ANPEP Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-ANPEP antibody (A02591-2). <br>Overlay histogram showing U937 cells stained with A02591-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ANPEP Antibody (A02591-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd13-anpep-picoband-trade-antibody-a02591-3-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-wb-testing-1.jpgAnti-CD13/Anpep Picoband® Antibody Western blot analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates,<br>
Lane 2: rat kidney tissue lysates,<br>
Lane 3: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD13/Anpep antigen affinity purified polyclonal antibody (Catalog # A02591-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD13/Anpep at approximately 150 kDa. The expected band size for CD13/Anpep is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-ihc-testing-2.jpgAnti-CD13/Anpep Picoband® Antibody IHC analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-ihc-testing-3.jpgAnti-CD13/Anpep Picoband® Antibody IHC analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-ihc-testing-4.jpgAnti-CD13/Anpep Picoband® Antibody IHC analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-if-testing-5.jpgAnti-CD13/Anpep Picoband® Antibody IF analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-if-testing-6.jpgAnti-CD13/Anpep Picoband® Antibody IF analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-if-testing-7.jpgAnti-CD13/Anpep Picoband® Antibody IF analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02591-3-cd13-primary-antibodies-if-testing-8.jpgAnti-CD13/Anpep Picoband® Antibody IF analysis of CD13/Anpep using anti-CD13/Anpep antibody (A02591-3). <br>
CD13/Anpep was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD13/Anpep Antibody (A02591-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-asah1-picoband-trade-antibody-a02055-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-wb-testing-1.jpgAnti-ASAH1 Picoband® Antibody Western blot analysis of ASAH1 using anti-ASAH1 antibody (A02055-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: rat heart tissue lysates, <br>
Lane 3: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASAH1 antigen affinity purified polyclonal antibody (Catalog # A02055-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASAH1 at approximately 45KD. The expected band size for ASAH1 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-ihc-testing-2.jpgAnti-ASAH1 Picoband® Antibody IHC analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). <br>
ASAH1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASAH1 Antibody (A02055-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-ihc-testing-3.jpgAnti-ASAH1 Picoband® Antibody IHC analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). <br>
ASAH1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASAH1 Antibody (A02055-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-ihc-testing-4.jpgAnti-ASAH1 Picoband® Antibody IHC analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). <br>
ASAH1 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASAH1 Antibody (A02055-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-ihc-testing-5.jpgAnti-ASAH1 Picoband® Antibody IHC analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). <br>
ASAH1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASAH1 Antibody (A02055-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02055-1-asah1-primary-antibodies-icc-testing-6.jpgAnti-ASAH1 Picoband® Antibody IF analysis of ASAH1 using anti-ASAH1 antibody (A02055-1). <br>
ASAH1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ASAH1 Antibody (A02055-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ass1-picoband-trade-antibody-a02212-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02212-1-ass1-primary-antibodies-wb-testing-1_1.jpgAnti-ASS1 Picoband® Antibody Western blot analysis of ASS1 using anti-ASS1 antibody (A02212-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: human MDA-MB-453 whole cell lysates, <br>
Lane 5: human A431 whole cell lysates, <br>
Lane 6: human HL-60 whole cell lysates, <br>
Lane 7: human U87 whole cell lysates, <br>
Lane 8: rat kidney tissue lysates, <br>
Lane 9: rat liver tissue lysates, <br>
Lane 10: mouse kidney tissue lysates, <br>
Lane 11: mouse liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASS1 antigen affinity purified polyclonal antibody (Catalog # A02212-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02212-1-ass1-primary-antibodies-ihc-testing-2.jpgAnti-ASS1 Picoband® Antibody IHC analysis of ASS1 using anti-ASS1 antibody (A02212-1). <br>
ASS1 was detected in paraffin-embedded section of human Pancreatic Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASS1 Antibody (A02212-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02212-1-ass1-primary-antibodies-icc-testing-3.jpgAnti-ASS1 Picoband® Antibody IF analysis of ASS1 using anti-ASS1 antibody (A02212-1). <br>
ASS1 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ASS1 Antibody (A02212-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02212-1-ass1-primary-antibodies-fc-testing-4.pngAnti-ASS1 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-ASS1 antibody (A02212-1). <br>Overlay histogram showing THP-1 cells stained with A02212-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASS1 Antibody (A02212-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-bambi-nma-picoband-trade-antibody-a07983-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07983-1-bambi-primary-antibodies-wb-testing-1.jpgAnti-BAMBI/NMA Picoband® Antibody Western blot analysis of BAMBI using anti-BAMBI antibody (A07983-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAMBI antigen affinity purified polyclonal antibody (Catalog # A07983-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAMBI at approximately 29KD. The expected band size for BAMBI is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-carbonic-anhydrase-4-ca4-picoband-trade-antibody-a01523-3-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01523-3-ca4-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase 4/CA4 Picoband® Antibody Western blot analysis of Ca4 using anti-Ca4 antibody (A01523-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: mouse lung tissue lysates, <br>
Lane 2: rat PC-12 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ca4 antigen affinity purified polyclonal antibody (Catalog # A01523-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ca4 at approximately 35KD. The expected band size for Ca4 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01523-3-ca4-primary-antibodies-ihc-testing-2.jpgAnti-Carbonic Anhydrase 4/CA4 Picoband® Antibody IHC analysis of Ca4 using anti-Ca4 antibody (A01523-3). <br>
Ca4 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ca4 Antibody (A01523-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01523-3-ca4-primary-antibodies-ihc-testing-3.jpgAnti-Carbonic Anhydrase 4/CA4 Picoband® Antibody IHC analysis of Ca4 using anti-Ca4 antibody (A01523-3). <br>
Ca4 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ca4 Antibody (A01523-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01523-3-ca4-primary-antibodies-fc-testing-4.pngAnti-Carbonic Anhydrase 4/CA4 Picoband® Antibody Flow Cytometry analysis of mouse spleen cells using anti-Ca4 antibody (A01523-3). <br>
Overlay histogram showing mouse spleen cells stained with A01523-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ca4 Antibody (A01523-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-card9-picoband-trade-antibody-a01410-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01410-1-card9-primary-antibodies-wb-testing-1_1.jpgAnti-CARD9 Picoband® Antibody Western blot analysis of CARD9 using anti-CARD9 antibody (A01410-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD9 antigen affinity purified polyclonal antibody (Catalog # A01410-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD9 at approximately 63 kDa. The expected band size for CARD9 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01410-1-card9-primary-antibodies-ihc-testing-2.jpgAnti-CARD9 Picoband® Antibody IHC analysis of CARD9 using anti-CARD9 antibody (A01410-1). <br>
CARD9 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CARD9 Antibody (A01410-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01410-1-card9-primary-antibodies-icc-testing-3.jpgAnti-CARD9 Picoband® Antibody IF analysis of CARD9 using anti-CARD9 antibody (A01410-1). <br>
CARD9 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CARD9 Antibody (A01410-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01410-1-card9-primary-antibodies-fc-testing-4.pngAnti-CARD9 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-CARD9 antibody (A01410-1). <br>Overlay histogram showing HEPA1-6 cells stained with A01410-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CARD9 Antibody (A01410-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01410-1-card9-primary-antibodies-fc-testing-5.pngAnti-CARD9 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-CARD9 antibody (A01410-1). <br>Overlay histogram showing THP-1 cells stained with A01410-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CARD9 Antibody (A01410-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cbr1-picoband-trade-antibody-a02825-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-wb-testing-1_1.jpgAnti-CBR1 Picoband® Antibody Western blot analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human SW620 whole cell lysates, <br>
Lane 2: human SK-OV-3 whole cell lysates, <br>
Lane 3: human 293T whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: human MCF-7 whole cell lysates, <br>
Lane 6: human SH-SY5Y whole cell lysates, <br>
Lane 7: rat testis tissue lysates, <br>
Lane 8: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified polyclonal antibody (Catalog # A02825-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 36 kDa. The expected band size for CBR1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-2_1.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-3.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-4.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-5.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-6.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-ihc-testing-7.jpgAnti-CBR1 Picoband® Antibody IHC analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-fcm-testing-9.jpgAnti-CBR1 Picoband® Antibody Flow Cytometry analysis of U87 cells using anti-CBR1 antibody (A02825-1). <br>Overlay histogram showing U87 cells stained with A02825-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBR1 Antibody (A02825-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-if-testing-8.jpgAnti-CBR1 Picoband® Antibody IF analysis of CBR1 using anti-CBR1 antibody (A02825-1). <br>
CBR1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CBR1 Antibody (A02825-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02825-1-cbr1-primary-antibodies-fcm-testing-10.jpgAnti-CBR1 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-CBR1 antibody (A02825-1). <br>Overlay histogram showing HEPA1-6 cells stained with A02825-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBR1 Antibody (A02825-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ccr2-picoband-trade-antibody-a00158-5-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-5-ccr2-primary-antibodies-wb-testing-1.jpgAnti-CCR2 Picoband® Antibody Western blot analysis of Ccr2 using anti-Ccr2 antibody (A00158-5).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccr2 antigen affinity purified polyclonal antibody (Catalog # A00158-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccr2 at approximately 45KD. The expected band size for Ccr2 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-5-ccr2-primary-antibodies-ihc-testing-2.jpgAnti-CCR2 Picoband® Antibody IHC analysis of Ccr2 using anti-Ccr2 antibody (A00158-5). <br>
Ccr2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ccr2 Antibody (A00158-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-5-ccr2-primary-antibodies-ihc-testing-3.jpgAnti-CCR2 Picoband® Antibody IHC analysis of Ccr2 using anti-Ccr2 antibody (A00158-5). <br>
Ccr2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ccr2 Antibody (A00158-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-ccr5-picoband-trade-antibody-a00061-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00061-2-ccr5-primary-antibodies-wb-testing-1.jpgAnti-CCR5 Picoband® Antibody Western blot analysis of CCR5 using anti-CCR5 antibody (A00061-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: mouse spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (Catalog # A00061-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR5 at approximately 40KD. The expected band size for CCR5 is at 40KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ccr6-picoband-trade-antibody-a00957-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00957-ccr6-primary-antibodies-ihc-testing-1.jpgAnti-CCR6 Antibody IHC analysis of CCR6 using anti-CCR6 antibody (A00957). <br>
CCR6 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR6 Antibody (A00957) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00957-gr4.jpgAnti-CCR6 AntibodySSD treatment ameliorated RA rats. Representative images of hind paws from different groups and changes in foot circumference. Hematoxylin eosin staining and immunohistochemical staining (CD31, CD39, CD73, CCR6 and IL1R1) of knee joint synovial slices. *P < 0.05, **P < 0.01 vs Model group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)13288-4'>39296024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00957-ccr6-primary-antibodies-ihc-testing-2.jpgAnti-CCR6 Antibody IHC analysis of CCR6 using anti-CCR6 antibody (A00957). <br>
CCR6 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR6 Antibody (A00957) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00957-ccr6-primary-antibodies-ihc-testing-3.jpgAnti-CCR6 Antibody IHC analysis of CCR6 using anti-CCR6 antibody (A00957). <br>
CCR6 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCR6 Antibody (A00957) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-muscarinic-acetylcholine-receptor-1-chrm1-antibody-a04081-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04081-chrm1-primary-antibodies-wb-testing-1.jpgAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody Picoband® Western blot analysis of CHRM1 using anti-CHRM1 antibody (A04081).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat C6 whole cell lysates, <br>
Lane 2: mouse Neuro-2A whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM1 antigen affinity purified polyclonal antibody (Catalog # A04081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM1 at approximately 55KD. The expected band size for CHRM1 is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04081-chrm1-primary-antibodies-ihc-testing-4.jpgAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody Picoband®IHC analysis of CHRM1 using anti-CHRM1 antibody (A04081). <br>CHRM1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHRM1 Antibody (A04081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04081-chrm1-primary-antibodies-ihc-testing-2.jpgAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody Picoband® IHC analysis of CHRM1 using anti-CHRM1 antibody (A04081). <br>
CHRM1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHRM1 Antibody (A04081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04081-chrm1-primary-antibodies-ihc-testing-3.jpgAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody Picoband® IHC analysis of CHRM1 using anti-CHRM1 antibody (A04081). <br>
CHRM1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHRM1 Antibody (A04081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04081-chrm1-primary-antibodies-fc-testing-4.pngAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CHRM1 antibody (A04081). <br>Overlay histogram showing PC-3 cells stained with A04081 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRM1 Antibody (A04081, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-dazl-picoband-trade-antibody-a02069-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-wb-testing-1.jpgAnti-DAZL Picoband® Antibody Western blot analysis of DAZL using anti-DAZL antibody (A02069-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: rat testicular tissue lysates, <br>
Lane 3: mouse testicular tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZL antigen affinity purified polyclonal antibody (Catalog # A02069-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZL at approximately 38KD. The expected band size for DAZL is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-12864_2024_10120_fig2_html.pngAnti-DAZL Picoband® AntibodyEffects of EZH2 interference or overexpression and JMJD3 interference or overexpression on self-renewal, proliferation and differentiation of spermatogonia. ( A ) The mRNA levels of PCNA, Cyclin-A, GFRA1, PLZF and C-KIT related to spermatogonia self-renewal and proliferation were changed after EZH2 and JMJD3 knockdown. ( B ) The expression of PCNA, cyclin-A, GFRA1, PLZF, C-KIT, DAZL and VASA was detected by qRT-PCR after EZH2 and JMJD3 overexpression. ( C ) The protein expression changes as well as statistical analysis of PCNA, DAZL and GFRA1 after EZH2 and JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig . ( D ) The cell cycle of EZH2 and JMJD3 overexpression cells was detected by flow cytometry. ( E ) Protein interaction network of EZH2, JMJD3 and spermatogonia self-renewal, proliferation and differentiation-related genes <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-ihc-testing-2.jpgAnti-DAZL Picoband® Antibody IHC analysis of DAZL using anti-DAZL antibody (A02069-2). <br>
DAZL was detected in paraffin-embedded section of human testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-ihc-testing-3.jpgAnti-DAZL Picoband® Antibody IHC analysis of DAZL using anti-DAZL antibody (A02069-2). <br>
DAZL was detected in paraffin-embedded section of mouse testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-ihc-testing-4.jpgAnti-DAZL Picoband® Antibody IHC analysis of DAZL using anti-DAZL antibody (A02069-2). <br>
DAZL was detected in paraffin-embedded section of rat testies cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZL Antibody (A02069-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-fc-testing-5.pngAnti-DAZL Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-DAZL antibody (A02069-2). <br>Overlay histogram showing HEPA1-6 cells stained with A02069-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAZL Antibody (A02069-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02069-2-dazl-primary-antibodies-fc-testing-6.pngAnti-DAZL Picoband® Antibody Flow Cytometry analysis of HL-60 cells using anti-DAZL antibody (A02069-2). <br>Overlay histogram showing HL-60 cells stained with A02069-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAZL Antibody (A02069-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-dusp3-picoband-trade-antibody-a06135-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06135-dusp3-primary-antibodies-wb-testing-1_1.jpgAnti-DUSP3 Picoband® Antibody Western blot analysis of DUSP3 using anti-DUSP3 antibody (A06135). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: rat skeletal muscle tissue lysates,<br>
Lane 4: rat heart tissue lysates,<br>
Lane 5: mouse skeletal muscle tissue lysates,<br>
Lane 6: mouse heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP3 antigen affinity purified polyclonal antibody (Catalog # A06135) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP3 at approximately 20 kDa. The expected band size for DUSP3 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06135-dusp3-primary-antibodies-fc-testing-2.pngAnti-DUSP3 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-DUSP3 antibody (A06135). <br>Overlay histogram showing THP-1 cells stained with A06135 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DUSP3 Antibody (A06135, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-egfr-picoband-trade-antibody-a00023-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-2-egfr-primary-antibodies-wb-testing-1.jpgAnti-EGFR Picoband® Antibody Western blot analysis of EGFR using anti-EGFR antibody (A00023-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat liver tissue lysates,<br>
Lane 2: mouse liver tissue lysates,<br>
Lane 3: mouse spleen tissue lysates,<br>
Lane 4: mouse lung tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # A00023-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 180KD. The expected band size for EGFR is at 180KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-2-12935_2021_2277_fig9_html.pngAnti-EGFR Picoband® AntibodyRHBDF2 related functions were mediated by EGFR signaling pathway. a , b Phosphorylation of EGFR and PD-L1 protein level in 786-O and 769-P cells were detected by western blot. Significance testing of gray statistics was analyzed by two-way ANOVA. c Growth rate of the transplanted tumor in the control group and RHBDF2 knockdown group (data were presented as the mean ± SEM and subjected to two-way ANOVA for significance test). d Representative images of xenografts in nude mice. e Immunofluorescent staining of phosphorylation of EGFR in the graft sections (The horizontal line at the bottom right represents 50 microns). f The mean fluorescence intensity of the phosphorylation staining of EGFR in the graft sections (t-test). g The migratory ability testing of 786-O cells and 769-P cells after Gefitinib treatment. h Statistics of the cell migratory ability after Gefitinib treatment (two-way ANOVA). i The detection of pEGFR and PD-L1 level in 786-O and 769-P cells after Gefitinib treatment. j Gray statistics of pEGFR and PD-L1 level in 786-O and 769-P cells (two-way ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-02277-0'>34736454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-2-egfr-primary-antibodies-ihc-testing-2.jpgAnti-EGFR Picoband® Antibody IHC analysis of EGFR using anti-EGFR antibody (A00023-2).<br>
EGFR was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGFR Antibody (A00023-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00023-2-egfr-primary-antibodies-ihc-testing-3.jpgAnti-EGFR Picoband® Antibody IHC analysis of EGFR using anti-EGFR antibody (A00023-2).<br>
EGFR was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGFR Antibody (A00023-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-epb41l5-antibodya09638-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09638-2-epb41l5-primary-antibodies-wb-testing-1.jpgAnti-Band 4.1-like protein 5 EPB41L5 Antibody Picoband® Western blot analysis of EPB41L5 using anti-EPB41L5 antibody (A09638-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB41L5 antigen affinity purified polyclonal antibody (Catalog # A09638-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPB41L5 at approximately 95KD. The expected band size for EPB41L5 is at 82KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09638-2-epb41l5-primary-antibodies-ihc-testing-2.jpgAnti-Band 4.1-like protein 5 EPB41L5 Antibody Picoband® IHC analysis of EPB41L5 using anti-EPB41L5 antibody (A09638-2). <br>
EPB41L5 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EPB41L5 Antibody (A09638-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09638-2-epb41l5-primary-antibodies-ihc-testing-3.jpgAnti-Band 4.1-like protein 5 EPB41L5 Antibody Picoband® IHC analysis of EPB41L5 using anti-EPB41L5 antibody (A09638-2). <br>
EPB41L5 was detected in paraffin-embedded section of human Ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EPB41L5 Antibody (A09638-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09638-2-epb41l5-primary-antibodies-fc-testing-4.pngAnti-Band 4.1-like protein 5 EPB41L5 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-EPB41L5 antibody (A09638-2). <br>Overlay histogram showing A431 cells stained with A09638-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPB41L5 Antibody (A09638-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-erf1-etf1-picoband-trade-antibody-a04157-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04157-2-etf1-primary-antibodies-wb-testing-1.jpgAnti-eRF1/ETF1 Picoband® Antibody Western blot analysis of ETF1 using anti-ETF1 antibody (A04157-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: mouse NIH3T3 whole cell lysates, <br>
Lane 4: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF1 antigen affinity purified polyclonal antibody (Catalog # A04157-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF1 at approximately 49KD. The expected band size for ETF1 is at 49KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fdps-fps-picoband-trade-antibody-a01782-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01782-1-fdps-primary-antibodies-wb-testing-1.jpgAnti-FDPS/FPS Picoband® Antibody Western blot analysis of FDPS using anti-FDPS antibody (A01782-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FDPS antigen affinity purified polyclonal antibody (Catalog # A01782-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FDPS at approximately 40KD. The expected band size for FDPS is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01782-1-fdps-primary-antibodies-fc-testing-2.pngAnti-FDPS/FPS Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-FDPS antibody (A01782-1). <br>Overlay histogram showing THP-1 cells stained with A01782-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FDPS Antibody (A01782-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fdps-fps-picoband-trade-antibody-a01782-2-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01782-2-fdps-primary-antibodies-wb-testing-1.jpgAnti-FDPS/FPS Picoband® Antibody Western blot analysis of Fdps using anti-Fdps antibody (A01782-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fdps antigen affinity purified polyclonal antibody (Catalog # A01782-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fdps at approximately 40KD. The expected band size for Fdps is at 40KD.
https://www.bosterbio.com/products/primary-antibodies/anti-flcn-picoband-trade-antibody-a00718-1-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-wb-testing-1_1.jpgAnti-FLCN Picoband® Antibody Western blot analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: human A549 whole cell lysates,<br>
Lane 4: rat kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLCN antigen affinity purified polyclonal antibody (Catalog # A00718-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLCN at approximately 70 kDa. The expected band size for FLCN is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-if-testing-2.jpgAnti-FLCN Picoband® Antibody IF analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-fc-testing-3.pngAnti-FLCN Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-FLCN antibody (A00718-1). <br>Overlay histogram showing U937 cells stained with A00718-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLCN Antibody (A00718-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-4.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-5.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-6.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-7.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-8.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-9.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00718-1-flcn-primary-antibodies-ihc-testing-10.jpgAnti-FLCN Picoband® Antibody IHC analysis of FLCN using anti-FLCN antibody (A00718-1). <br>
FLCN was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLCN Antibody (A00718-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-fli1-picoband-trade-antibody-a00399-boster.html2026-03-24T05:26:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00399-fli1-primary-antibodies-wb-testing-1.jpgAnti-FLI1 Picoband® Antibody Western blot analysis of FLI1 using anti-FLI1 antibody (A00399).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates, <br>
Lane 3: rat liver tissue lysates, <br>
Lane 4: mouse liver tissue lysates, <br>
Lane 5: mouse lung tissue lysates, <br>
Lane 6: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLI1 antigen affinity purified polyclonal antibody (Catalog # A00399) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLI1 at approximately 54KD. The expected band size for FLI1 is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00399-fli1-primary-antibodies-ihc-testing-2.jpgAnti-FLI1 Picoband® Antibody IHC analysis of FLI1 using anti-FLI1 antibody (A00399). <br>
FLI1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FLI1 Antibody (A00399) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00399-fli1-primary-antibodies-icc-testing-3.jpgAnti-FLI1 Picoband® Antibody IF analysis of FLI1 using anti-FLI1 antibody (A00399). <br>
FLI1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FLI1 Antibody (A00399) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00399-fli1-primary-antibodies-fc-testing-4.pngAnti-FLI1 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-FLI1 antibody (A00399). <br>Overlay histogram showing THP-1 cells stained with A00399 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FLI1 Antibody (A00399, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-foxe1-picoband-trade-antibody-a02831-5-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-foxe1-primary-antibodies-wb-testing-1_1.jpgAnti-FOXE1 Picoband® Antibody Western blot analysis of FOXE1 using anti-FOXE1 antibody (A02831-5). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human SiHa whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXE1 antigen affinity purified polyclonal antibody (A02831-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXE1 at approximately 38 kDa. The expected band size for FOXE1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g001.jpgAnti-FOXE1 Picoband® AntibodyFoxe1 protein structure in human, mouse and zebrafish. Multiple sequence alignment of full-length human, mouse and zebrafish FOXE1 protein. The protein contains three characterized domains which are annotated with colored lines. The similarity of the Forkhead domain (FHD) is 97% and 100%, respectively, between zebrafish and humans and between mouse and human. The sequence similarity of the whole protein between human and zebrafish is 54%. NLS is 100% identical between all species and the poly alanine stretch (PAS) is only present in humans and mouse. Red boxes mark the putative DNA-binding residues and asterisks (*) mark locations of missense mutations that were reported in individuals with Bamforth–Lazarus syndrome ( ; ; ; ; ; ). NLS: nuclear localization signal, FHD: forkhead box domain PAS: poly alanine stretch in human and mouse FOXE1. Alignment made using Clustal Omega.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g002.jpgAnti-FOXE1 Picoband® AntibodyFoxe1 is expressed in a range of larval tissues, including the oral epithelium, ethmoid plate, and the brain. (A–H) foxe1 transcripts were detected diffusely throughout the embryo from early development at 12.5 hpf (A) which continued through early larval stages (B–D) where transcripts also seemed specifically upregulated in the subpharyngeal area (E, F) before being enhanced in the ceratobranchials and ceratohyal ( (G) , insert lateral view). (A-D) lateral view and (E-H) ventral view. (I) Normalized relative expression of foxe1 during embryonic and larval stages as determined by qPCR. Error bars indicate standard deviations. (J-Q) Detailed protein localization at 96 hpf showed Foxe1-positive cells in the brain (J) , the ceratobranchials (K) , in the oral epithelium and in a linear cluster in the subpharyngeal area (white arrow) (L) , the eyes (M) , on the ethmoid plate (N) , on the lining of the ceratohyal (O) , in the mouth opening (P) and to some extent in the fin tips (Q) , upper image) and in the notochordal sheath ( (Q) , lower image). Posterior-anterior axis in J applies to all images. cb: ceratobranchals, ch: ceratohyal. A-H scalebar 100 μm, (J-Q) scalebar 50 µm. Antibody validation in .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g003.jpgAnti-FOXE1 Picoband® AntibodyFoxe1 and T4 are colocalized in the subpharyngeal area. Foxe1 and T4 staining on adjacent coronal sections of the subpharyngeal area of the larval head. T4 and Foxe1 staining in the developing thyroid follicles at 96 hpf. Nuclear counterstain with DAPI. cb; ceratobranchials, e; eye. Scale bar 200 µm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g004.jpgAnti-FOXE1 Picoband® AntibodyThe foxe1 rdb2 mutant. (A) Wild type and foxe1 rdb2 mutant DNA sequence at (C) 84–105. The protospacer adjacent motif is underlined (full gRNA sequence: 5′-GCCGCAAAGAGGCCGTCGGAGG-3′). The deletion is highlighted in red and the insertion in green. (B) Schematic representation of the zebrafish Foxe1 protein and the position of the amino acid changes as indicated by the red bars. (C) Nuclear localization of wild type Foxe1 and cytoplasmatic localization of mutant Foxe1 in zebrafish keratinocytes on scale. Scale bar 50 µm. (D) Representative images of wild types and foxe1 mutants at 6 dpf and 2 months post fertilization. Scale bars 1 mm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g005.jpgAnti-FOXE1 Picoband® Antibodyfoxe1 mutants show upregulated Foxe1 expression. Foxe1 expression in the oral epithelium and the ethmoid plate of wild type and foxe1 mutants at 96 hpf. Foxe1 was more abundantly expressed in the mutants compared to the wild types, but the tissue localization of the protein remained unaffected. Scale bar 50 µm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g006.jpgAnti-FOXE1 Picoband® AntibodyThyroid follicle development in foxe1 mutants. (A) Normalized gene-expression of thyroid marker thyroglobulin during early development (3 dpf). (B, D) Analysis of T4 positive follicles by whole mount immunohistochemistry in 6 dpf larvae showed no difference in mean follicle count or volume. (C) Representative images of wild type and mutant thyroid follicles. Larvae were imaged from the ventral side and T4-positive follicle surfaces were rendered from z-stack images using Imaris 9.0 as previously described ( ). Scale bar 200 µm. (D) Data were assessed for normality with the D’Agostino-Pearson normality test. Normally distributed data were analyzed for statistical differences using a one-way ANOVA and post hoc Tukey test or unpaired t -test. Non-parametric data were compared with a Kruskal–Wallis test with post hoc Dunn’s Multiple comparison test or Mann-Whitney test. Error bars indicate standard deviation.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g007.jpgAnti-FOXE1 Picoband® AntibodyMalformed ceratohyal cartilage and reduced content of Ca, Mg and P in foxe1 mutants. (A) Representative images of wild types and foxe1 heterozygous- and homozygous mutants, ventral and lateral view. (B) High magnification images of ceratohyal phenotype. (B′) Col2a1a positive cells in the in ceratohyal. (C) Standard length at 8 dpf. (D) Count of mineralized vertebrae in foxe1 mutants versus wild types at 8 dpf. (E–G) Molar calcium, magnesium and phosphorus content in foxe1 mutants versus wild type larvae during early skeletal development three to 8 dpf. n = 10–33. Scale bar 200 µm. Data were assessed for normality with the D’Agostino-Pearson normality test. Normally distributed data were analyzed for statistical differences using a one-way ANOVA and post-hoc Tukey test or unpaired t -test. Non-parametric data were compared with a Kruskal–Wallis test with post-hoc Dunn’s Multiple comparison test or Mann-Whitney test. Error bars indicate standard deviation.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-fcell-11-1143844-g008.jpgAnti-FOXE1 Picoband® AntibodyGene expression of cartilage and bone (precursor) cells is affected in foxe1 mutants. (A-I) Relative gene expression of tgfβ3, wnt5a, sp7, col2a1, col1a2, runx2b, dlx2a, sox9a and fgfr2 in mutants versus wild types. Axis description from (A) applies to all graphs. (J) dlx2a positive post migratory neural crest cells at 24 hpf (Prim-5) in wild types, 20% of the heterozygous- and 60% of the homozygous mutants. Asterisks indicate the level of significance: * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001 ( n = 10–15). Data were assessed for normality with the D’Agostino-Pearson normality test. Normally distributed data were analyzed using a one-way ANOVA and post-hoc Tukey test. Non-parametric data were compared with a Kruskal–Wallis test with post-hoc Dunn’s Multiple comparison test. Error bars indicate standard deviation. Scale bar 100 µm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1143844/full'>36994096</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-foxe1-primary-antibodies-fc-testing-2.pngAnti-FOXE1 Picoband® Antibody Flow Cytometry analysis of SiHa cells using anti-FOXE1 antibody (A02831-5). <br>Overlay histogram showing SiHa cells stained with A02831-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXE1 Antibody (A02831-5, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-foxf1-picoband-trade-antibody-a03563-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-foxf1-primary-antibodies-wb-testing-1.jpgAnti-FOXF1 Picoband® Antibody Western blot analysis of FOXF1 using anti-FOXF1 antibody (A03563-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates, <br>
Lane 4: human U2OS whole cell lysates, <br>
Lane 5: mouse liver tissue lysates, <br>
Lane 6: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXF1 antigen affinity purified polyclonal antibody (Catalog # A03563-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXF1 at approximately 38-40KD. The expected band size for FOXF1 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-12885_2022_9566_fig6_html.pngAnti-FOXF1 Picoband® AntibodyLINC00022 upregulated FOXF1 expression through sponging miR-375-3p. ( a ) The binding sites between LINC00022 and miR-375-3p were predicted by LncBase v.2 and verified by dual luciferase assay. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs wt-LINC00022 + miR-375-3p mimics group. After HCT116, DLD1, and CaCo-2 cells were infected with LINC00022 low expression lentivirus or high expression lentivirus, the relative mRNA levels of miR-375-3p and FOXF1 were assessed by qRT-PCR ( b ); ** P < 0.01, and *** P < 0.001 vs Lv-anti-NC group or Lv-NC group. Relative protein levels of FOXF1, STAT3, and p-STAT3 were calculated using Western blot ( c ). β-actin served as the internal control. ( d ) The binding sites between miR-375-3p and FOXF1 were predicted by TargetScanHuman 7.2 and verified by dual luciferase assay. ** P < 0.01 vs wt-FOXF1 + miR-375-3p mimics group. Data were presented as mean ± standard deviation (SD). N = 3. FOXF1, Forkhead Box F1; STAT3, signal transducer and activator of transcription 3, and qRT-PCR, quantitative Real-time PCR <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-022-09566-5'>35468741</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-12885_2022_9566_fig7_html.pngAnti-FOXF1 Picoband® AntibodymiR-375-3p knockdown reversed the effects of LINC00022 down-regulation on cell function in CRC. After LINC00022-silenced HCT116 cells were infected with Lv-anti-miR-375-3p mimic or Lv-anti-miR-NC, the viability of HCT116 cells was measured by CCK-8 assay ( a ); The migration (Scale bar = 200 μm) and invasion (Scale bar = 100 μm) of HCT116 cells were measured using Wound-healing assay and Transwell assay, respectively ( b and c ); Relative protein levels of FOXF1, c-Myc, pro caspase 3, cleaved caspase 3, MMP2, and VEGFA were assessed by Western blot ( d ). β-actin served as internal control. The content of VEGFA in cell supernatant was measured by detection kit ( e ). Data were presented as mean ± standard deviation (SD). N = 3. ** P < 0.01 vs Lv-anti-LINC00022 + Lv-anti-miR-NC group. NC, negative control; FOXF1, Forkhead Box F1; MMP2, matrix metalloproteinase 2; VEGFA, vascular endothelial growth factor-A <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-022-09566-5'>35468741</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-12885_2022_9566_fig8_html.pngAnti-FOXF1 Picoband® AntibodyLINC00022 promoted the development of CRC in vivo. HCT116 cells with stable low expression of LINC00022 or CaCo-2 cells with stable overexpression of LINC00022 were injected subcutaneously into nude mice. ( a ) Representative images of tumor tissues in nude mice. ( b ) The volumes were calculated after being injected with LINC00022-silenced cells or -overexpressed cells. ( c and d ) The mRNA levels of LINC00022, miR-375-3p, and FOXF1 were detected in tumor tissues. ( e ) Relative protein levels of c-Myc, pro caspase 3, cleaved caspase 3, MMP2, and VEGFA in tumor tissues. ( f ) Immunohistochemical staining of FOXF1 in tumor tissues. Scale bar = 50 μm. β-actin served as the internal control. Data were presented as mean ± standard deviation (SD). N = 6. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs Lv-anti-NC group or Lv-NC group. FOXF1, Forkhead Box F1; MMP2, matrix metalloproteinase 2; VEGFA, vascular endothelial growth factor-A <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-022-09566-5'>35468741</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-1-s2.0-s2213231725004069-gr5.jpgAnti-FOXF1 Picoband® AntibodyFOXF1 expression is reduced in fibrotic lungs. A and B, Representative immunofluorescence images of FOXF1 (A) and immunohistochemistry images (B) from non-PF and PF lung sections. Scale bars, 50 μm. C and D, Mouse lungs were collected at days 1, 3, 7, 10, 14, and 21 post-BLM instillation, and FOXF1 protein levels were analyzed by Western blot (C) and quantified using ImageJ (D). n = 6–8/group. Statistical analyses were performed using t-tests.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2213231725004069'>41101211</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-1-s2.0-s2213231725004069-gr6.jpgAnti-FOXF1 Picoband® AntibodyIron and ROS synergistically suppress FOXF1 levels in human lung fibroblasts. Cells were treated under the indicated conditions for 24 h. A, FOXF1 protein expression and quantification following FAC or FeSO4 treatment. B, FOXF1 mRNA levels determined by RT-PCR using 18S rRNA as an internal control. C, Intracellular ROS levels measured by DCFH-DA following BLM + FAC treatment. D, LIP levels measured using FeRhoNox-1. E, FOXF1 protein expression and quantification with the indicated treatments. F, ROS levels analyzed by flow cytometry with DCFH-DA following BLM + NAC treatment. G, LIP levels determined using FeRhoNox-1 by flow cytometry. H, FOXF1 protein expression and quantification with indicated treatments. FAC (200 μM), FeSO4 (100 μM), BLM (40 μg/mL), NAC (2 mM); n ≥ 3/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2213231725004069'>41101211</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-1-s2.0-s2213231725004069-gr7.jpgAnti-FOXF1 Picoband® AntibodyFOXF1 reduces ROS, iron levels, and COL1A1 transcripts in BLM-treated human lung fibroblasts. Cells were treated for 24 h with BLM combined with either FOXF1 overexpression or FOXF1 silencing (by siFOXF1-1), and ROS and LIP levels were analyzed by flow cytometry using DCFH-DA and FeRhoNox-1, respectively. A, FOXF1 protein expression and quantification following FOXF1 overexpression. B, Intracellular ROS levels measured by flow cytometry. C, FOXF1 protein expression and quantification following FOXF1 silencing. D, ROS levels following FOXF1 silencing. E, LIP levels after FOXF1 overexpression. F, LIP levels after FOXF1 silencing. G, COL1A1 mRNA levels analyzed by real-time PCR using β-actin as an internal control. BLM, 40 μg/mL. n ≥ 3/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2213231725004069'>41101211</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-1-s2.0-s2213231725004069-gr8.jpgAnti-FOXF1 Picoband® AntibodyFOXF1 promotes antioxidant protein expression, and FDX1 decreases ROS, Fe2+ levels, and COL1A1 in BLM-treated human primary lung fibroblasts. A, The protein levels of FDX1 and HO-1 were analyzed by Western blot in FOXF1-overexpressing fibroblasts. n = 3/group. B, Protein expression following FOXF1 silencing. n = 3–4/group. C, Western blot analysis of FDX1 protein levels in lungs from BLM-treated WT and tfr1+/− mice with t-test. n = 6/group. D–G, Human fibroblasts treated for 24 h with BLM and FDX1 overexpression were analyzed for FDX1 mRNA levels by RT-PCR with β-actin as the internal control (D), ROS levels by DCFH-DA probe and flow cytometry (E), LIP levels by FeRhoNox-1 and flow cytometry (F), and COL1A1 mRNA levels by real-time PCR with β-actin as the internal control (G). BLM, 40 μg/mL n = 3/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2213231725004069'>41101211</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-foxf1-primary-antibodies-ihc-testing-2.jpgAnti-FOXF1 Picoband® Antibody IHC analysis of FOXF1 using anti-FOXF1 antibody (A03563-1). <br>
FOXF1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOXF1 Antibody (A03563-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-foxf1-primary-antibodies-ihc-testing-3.jpgAnti-FOXF1 Picoband® Antibody IHC analysis of FOXF1 using anti-FOXF1 antibody (A03563-1). <br>
FOXF1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOXF1 Antibody (A03563-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-foxf1-primary-antibodies-ihc-testing-4.jpgAnti-FOXF1 Picoband® Antibody IHC analysis of FOXF1 using anti-FOXF1 antibody (A03563-1). <br>
FOXF1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOXF1 Antibody (A03563-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03563-1-foxf1-primary-antibodies-fc-testing-5.pngAnti-FOXF1 Picoband® Antibody Flow Cytometry analysis of Jurkat cells using anti-FOXF1 antibody (A03563-1). <br>
Overlay histogram showing Jurkat cells stained with A03563-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXF1 Antibody (A03563-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-foxi1-antibodya07512-3-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07512-3-foxi1-primary-antibodies-wb-testing-1.jpgAnti-Forkhead box protein I1 FOXI1 Antibody Picoband® Western blot analysis of FOXI1 using anti-FOXI1 antibody (A07512-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat kidney tissue lysates, <br>
Lane 2: monkey COS-7 cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXI1 antigen affinity purified polyclonal antibody (Catalog # A07512-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXI1 at approximately 60KD. The expected band size for FOXI1 is at 41KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07512-3-foxi1-primary-antibodies-icc-testing-2.jpgAnti-Forkhead box protein I1 FOXI1 Antibody Picoband® IF analysis of FOXI1 using anti-FOXI1 antibody (A07512-3). <br>
FOXI1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FOXI1 Antibody (A07512-3) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07512-3-foxi1-primary-antibodies-fc-testing-3.pngAnti-Forkhead box protein I1 FOXI1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-FOXI1 antibody (A07512-3). <br>Overlay histogram showing A549 cells stained with A07512-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXI1 Antibody (A07512-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-foxk1-mnf-picoband-trade-antibody-a07015-2-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-wb-testing-1_1.jpgAnti-FOXK1/MNF Picoband® Antibody Western blot analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human U20S whole cell lysates, <br>
Lane 4: human A431 whole cell lysates, <br>
Lane 5: human Hela whole cell lysates, <br>
Lane 6: human T-47D whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates, <br>
Lane 8: human HL-60 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXK1 antigen affinity purified polyclonal antibody (Catalog # A07015-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXK1 at approximately 97 kDa. The expected band size for FOXK1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-wb-testing-2.jpgAnti-FOXK1/MNF Picoband® Antibody Western blot analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat heart tissue lysates, <br>
Lane 3: rat RH-35 whole cell lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse SP2/0 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXK1 antigen affinity purified polyclonal antibody (Catalog # A07015-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXK1 at approximately 97 kDa. The expected band size for FOXK1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-3_1.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human ovarian adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-4.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-5.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human breast carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-fcm-testing-11.jpgAnti-FOXK1/MNF Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-FOXK1 antibody (A07015-2). <br>Overlay histogram showing THP-1 cells stained with A07015-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXK1 Antibody (A07015-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-6.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-7.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human cervical intraepithelial neoplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-8.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-ihc-testing-9.jpgAnti-FOXK1/MNF Picoband® Antibody IHC analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07015-2-foxk1-primary-antibodies-if-testing-10.jpgAnti-FOXK1/MNF Picoband® Antibody IF analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). <br>
FOXK1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXK1 Antibody (A07015-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-growth-hormone-receptor-ghr-picoband-trade-antibody-a00698-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-ghr-primary-antibodies-wb-testing-1.jpgAnti-Growth hormone receptor/GHR Picoband® Antibody Western blot analysis of GHR using anti-GHR antibody (A00698).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human SW620 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human Jurkat whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-ghr-primary-antibodies-ihc-testing-1.jpgAnti-Growth hormone receptor/GHR Picoband® AntibodyIHC analysis of GHR using anti-GHR antibody (A00698). <br>GHR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHR Antibody (A00698) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-ghr-primary-antibodies-icc-testing-2.jpgAnti-Growth hormone receptor/GHR Picoband® Antibody IF analysis of GHR using anti-GHR antibody (A00698). <br>
GHR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GHR Antibody (A00698) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-ghr-primary-antibodies-fc-testing-3.pngAnti-Growth hormone receptor/GHR Picoband® Antibody Flow Cytometry analysis of HepG2 cells using anti-GHR antibody (A00698). <br>Overlay histogram showing HepG2 cells stained with A00698 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GHR Antibody (A00698, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-growth-hormone-receptor-ghr-picoband-trade-antibody-a00698-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-1-ghr-primary-antibodies-wb-testing-1.jpgAnti-Growth hormone receptor/GHR Picoband® Antibody Western blot analysis of Ghr using anti-Ghr antibody (A00698-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat C6 whole cell lysates, <br>
Lane 2: mouse RAW246.7 whole cell lysates, <br>
Lane 3: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ghr antigen affinity purified polyclonal antibody (Catalog # A00698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ghr at approximately 130KD. The expected band size for Ghr is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-1-ghr-primary-antibodies-fc-testing-2.pngAnti-Growth hormone receptor/GHR Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-Ghr antibody (A00698-1). <br>Overlay histogram showing HEPA1-6 cells stained with A00698-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ghr Antibody (A00698-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00698-1-ghr-primary-antibodies-fc-testing-3.pngAnti-Growth hormone receptor/GHR Picoband® Antibody Flow Cytometry analysis of NRK cells using anti-Ghr antibody (A00698-1). <br>Overlay histogram showing NRK cells stained with A00698-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ghr Antibody (A00698-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gp2-picoband-trade-antibody-a06630-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06630-1-gp2-primary-antibodies-wb-testing-1.jpgAnti-GP2 Picoband® Antibody Western blot analysis of Gp2 using anti-Gp2 antibody (A06630-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: mouse kidney tissue lysates, <br>
Lane 2: mouse brain tissue lysates, <br>
Lane 3: mouse heart tissue lysates, <br>
Lane 4: rat kidney tissue lysates, <br>
Lane 5: rat brain tissue lysates, <br>
Lane 6: rat heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gp2 antigen affinity purified polyclonal antibody (Catalog # A06630-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gp2 at approximately 55KD. The expected band size for Gp2 is at 59KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06630-1-gp2-primary-antibodies-ihc-testing-2.jpgAnti-GP2 Picoband® Antibody IHC analysis of Gp2 using anti-Gp2 antibody (A06630-1). <br>
Gp2 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gp2 Antibody (A06630-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06630-1-gp2-primary-antibodies-fc-testing-3.pngAnti-GP2 Picoband® Antibody Flow Cytometry analysis of Neuro-2a cells using anti-Gp2 antibody (A06630-1). <br>Overlay histogram showing Neuro-2a cells stained with A06630-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gp2 Antibody (A06630-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gpnmb-gpnmb-picoband-trade-antibody-a02439-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-wb-testing-1_1.jpgAnti-GPNMB/Gpnmb Picoband® Antibody Western blot analysis of Gpnmb using anti-Gpnmb antibody (A02439-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: mouse liver tissue lysates, <br>
Lane 3: mouse kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gpnmb antigen affinity purified polyclonal antibody (Catalog # A02439-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gpnmb at approximately 64KD. The expected band size for Gpnmb is at 64KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-ihc-testing-2.jpgAnti-GPNMB/Gpnmb Picoband® Antibody IHC analysis of Gpnmb using anti-Gpnmb antibody (A02439-1). <br>
Gpnmb was detected in paraffin-embedded section of human liver cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-ihc-testing-3.jpgAnti-GPNMB/Gpnmb Picoband® Antibody IHC analysis of Gpnmb using anti-Gpnmb antibody (A02439-1). <br>
Gpnmb was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-icc-testing-4.jpgAnti-GPNMB/Gpnmb Picoband® Antibody IF analysis of Gpnmb using anti-Gpnmb antibody (A02439-1). <br>
Gpnmb was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-fc-testing-5.pngAnti-GPNMB/Gpnmb Picoband® Antibody Flow Cytometry analysis of U87 cells using anti-Gpnmb antibody (A02439-1). <br>Overlay histogram showing U87 cells stained with A02439-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gpnmb Antibody (A02439-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02439-1-gpnmb-primary-antibodies-fc-testing-6.pngAnti-GPNMB/Gpnmb Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-Gpnmb antibody (A02439-1). <br>Overlay histogram showing HEPA1-6 cells stained with A02439-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gpnmb Antibody (A02439-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd168-hmmr-picoband-trade-antibody-a05056-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05056-1-hmmr-primary-antibodies-wb-testing-1_1.jpgAnti-CD168/HMMR Picoband® Antibody Western blot analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human SW620 whole cell lysates, <br>
Lane 4: human T-47D whole cell lysates, <br>
Lane 5: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD168/HMMR antigen affinity purified polyclonal antibody (Catalog # A05056-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD168/HMMR at approximately 84KD. The expected band size for CD168/HMMR is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05056-1-hmmr-primary-antibodies-fc-testing-2.pngAnti-CD168/HMMR Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-HMMR antibody (A05056-1). <br>Overlay histogram showing U937 cells stained with A05056-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMMR Antibody (A05056-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05056-1-hmmr-primary-antibodies-if-testing-3.jpgAnti-CD168/HMMR Picoband® Antibody IF analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1). <br>
CD168/HMMR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD168/HMMR Antibody (A05056-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05056-1-hmmr-primary-antibodies-if-testing-4.jpgAnti-CD168/HMMR Picoband® Antibody IF analysis of CD168/HMMR using anti-CD168/HMMR antibody (A05056-1). <br>
CD168/HMMR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD168/HMMR Antibody (A05056-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-kdm6b-jmjd3-picoband-trade-antibody-a01309-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-kdm6b-primary-antibodies-wb-testing-1.jpgAnti-KDM6B/JMJD3 Picoband® Antibody Western blot analysis of KDM6B using anti-KDM6B antibody (A01309-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human A375 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human A431 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM6B antigen affinity purified polyclonal antibody (Catalog # A01309-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM6B at approximately 177KD. The expected band size for KDM6B is at 177KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig1_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyExpression levels of EZH2, JMJD3, H3K27me3 in spermatogonia. ( A ) qRT-PCR was used to detect the expression of EZH2 siRNA and JMJD3 siRNA in spermatogonia after interference. ( B ) measurements of EZH2 and JMJD3 mRNA levels in spermatogonia after overexpression. ( C ) The protein levels of EZH2, JMJD3 and H3K27me3 in spermatogonia were detected and statistically analyzed by Western blot. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig2_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyEffects of EZH2 interference or overexpression and JMJD3 interference or overexpression on self-renewal, proliferation and differentiation of spermatogonia. ( A ) The mRNA levels of PCNA, Cyclin-A, GFRA1, PLZF and C-KIT related to spermatogonia self-renewal and proliferation were changed after EZH2 and JMJD3 knockdown. ( B ) The expression of PCNA, cyclin-A, GFRA1, PLZF, C-KIT, DAZL and VASA was detected by qRT-PCR after EZH2 and JMJD3 overexpression. ( C ) The protein expression changes as well as statistical analysis of PCNA, DAZL and GFRA1 after EZH2 and JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig . ( D ) The cell cycle of EZH2 and JMJD3 overexpression cells was detected by flow cytometry. ( E ) Protein interaction network of EZH2, JMJD3 and spermatogonia self-renewal, proliferation and differentiation-related genes <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig3_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyThe manner in which EZH2, JMJD3 interacts with TET1 in spermatogonia. ( A ) The expression of EZH2 after EZH2 SiRNA in spermatogonia was detected by qRT-PCR. ( B ) qRT-PCR was used to detect the expression of EZH2 after co-transfection of EZH2 SiRNA and TET1 overexpression vector in spermatogonia. ( C ) The expression of JMJD3 in spermatogonia after JMJD3 SiRNA was detected by qRT-PCR. ( D ) qRT-PCR was used to detect the expression of JMJD3 after the co-transfection of JMJD3 SiRNA and TET1 overexpression vector in spermatogonia. ( E ) Bead plot of methylation sequencing results and methylation ratio after PCR amplification with primers designed for EZH2 and JMJD3(CpG-enriched region within the first 2000 bp of the promoter region), Filled circles represent methylated, empty circles represent unmethylated. ( F ) The JMJD3-TET1 protein interaction was detected by Co-IP technology. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig4_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyTET1 coordinates with H3K27me3 to target Pramel3 to promote its activation and expression. ( A ) qRT-PCR was used to detect the expression of genes enriched by Chip-seq after JMJD3 overexpression. ( B ) Peak plots of 2610002M06Rik and Pramel3 target genes obtained from TET1 overexpressing cells deposited with H3K27me3 antibody <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig5_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyTET1-H3K27me3 regulated through PI3K-AKT pathway. ( A ) KEGG enrichment analysis. ( B ) Western blot was used to detect the protein expressions of AKT and P-AKT in the PI3K-AKT pathway after JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-12864_2024_10120_fig6_html.pngAnti-KDM6B/JMJD3 Picoband® AntibodyIn vivo functional validation of JMJD3. ( A ) Spermatogenesis disorder model mice transplantation of control PCDH and JMJD3 Positive Cells. ( B ) Chart of comparison of control PCDH with testes transplanted with JMJD3 positive cells. ( C ) HE staining plot of JMJD3 overexpression versus control PCDH. ( D ) Expression of JMJD3 in testicular spermatogonia after overexpression of JMJD3. ( E ) Expression of H3K27me3 and PCNA in testicular spermatogonia after JMJD3 overexpression. ( F ) Statistical analysis of JMJD3 + cells/H3K27m3 + cells and PCNA + cells in immunohistochemistry. Scale bar = 50 μm. n = 3 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-kdm6b-primary-antibodies-fc-testing-2.pngAnti-KDM6B/JMJD3 Picoband® Antibody Flow Cytometry analysis of K562 cells using anti-KDM6B antibody (A01309-1). <br>Overlay histogram showing K562 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM6B Antibody (A01309-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01309-1-kdm6b-primary-antibodies-fc-testing-3.pngAnti-KDM6B/JMJD3 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-KDM6B antibody (A01309-1). <br>Overlay histogram showing HEPA1-6 cells stained with A01309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM6B Antibody (A01309-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lrrc32-picoband-trade-antibody-a08199-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08199-1-lrrc32-primary-antibodies-wb-testing-1.jpgAnti-LRRC32 Picoband® Antibody Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human SK-OV-3 whole cell lysates, <br>
Lane 2: human PANC-1 whole cell lysates, <br>
Lane 3: human 22RV1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mafa-picoband-trade-antibody-a05251-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05251-1-mafa-primary-antibodies-wb-testing-1.jpgAnti-MAFA Picoband® Antibody Western blot analysis of MAFA using anti-MAFA antibody (A05251-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody (Catalog # A05251-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFA at approximately 50KD. The expected band size for MAFA is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05251-1-mafa-primary-antibodies-ihc-testing-2.jpgAnti-MAFA Picoband® Antibody IHC analysis of MAFA using anti-MAFA antibody (A05251-1). <br>
MAFA was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAFA Antibody (A05251-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05251-1-mafa-primary-antibodies-ihc-testing-3.jpgAnti-MAFA Picoband® Antibody IHC analysis of MAFA using anti-MAFA antibody (A05251-1). <br>
MAFA was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAFA Antibody (A05251-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05251-1-mafa-primary-antibodies-fc-testing-4.pngAnti-MAFA Picoband® Antibody Flow Cytometry analysis of SiHa cells using anti-MAFA antibody (A05251-1). <br>
Overlay histogram showing SiHa cells stained with A05251-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAFA Antibody (A05251-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05251-1-mafa-primary-antibodies-if-testing-5.jpgAnti-MAFA Picoband® Antibody IF analysis of MAFA using anti-MAFA antibody (A05251-1). <br>
MAFA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MAFA Antibody (A05251-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-menin-men1-picoband-trade-antibody-a00331-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00331-1-men1-primary-antibodies-wb-testing-1_1.jpgAnti-Menin/MEN1 Picoband® AntibodyWestern blot analysis of Menin/MEN1 using anti-Menin/MEN1 antibody (A00331-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 5: rat testis tissue lysates,<br>
Lane 6: rat thymus tissue lysates,<br>
Lane 7: rat PC-12 whole cell lysates,<br>
Lane 7: mouse testis tissue lysates,<br>
Lane 8: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Menin/MEN1 antigen affinity purified polyclonal antibody (A00331-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Menin/MEN1 at approximately 68-70 kDa. The expected band size for Menin/MEN1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00331-1-men1-primary-antibodies-ihc-testing-1.jpgAnti-Menin/MEN1 Picoband® AntibodyIHC analysis of Menin/MEN1 using anti-Menin/MEN1 antibody (A00331-1). <br>Menin/MEN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Menin/MEN1 Antibody (A00331-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00331-1-men1-primary-antibodies-ihc-testing-2_1.jpgAnti-Menin/MEN1 Picoband® AntibodyIHC analysis of Menin/MEN1 using anti-Menin/MEN1 antibody (A00331-1). <br>Menin/MEN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Menin/MEN1 Antibody (A00331-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00331-1-men1-primary-antibodies-ihc-testing-3_1.jpgAnti-Menin/MEN1 Picoband® AntibodyIHC analysis of Menin/MEN1 using anti-Menin/MEN1 antibody (A00331-1). <br>Menin/MEN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Menin/MEN1 Antibody (A00331-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-mannose-receptor-mrc1-picoband-trade-antibody-a02285-2-boster.html2026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-wb-testing-1_1.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody Western blot analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates,<br>
Lane 2: mouse lung tissue lysates,<br>
Lane 3: human liver tissue lysates,<br>
Lane 4: monkey lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (Catalog # A02285-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-ihc-testing-2.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-12951_2025_3663_fig5_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyThe impact of SAB-PLGA@RTM on the polarization of M2 macrophages was verified in vitro. ( A ) Diagram of macrophage polarization. ( B ) Representative images and ( C ) quantitative analysis of α-SMA expression following treatment with various drugs ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( D ) Representative images of CD206, CXCR4, and CD86 expression in RAW 264.7 cells treated with different formulations. ( E-G ) Quantitative analysis of CD206, CXCR4, and CD86 expression in RAW 264.7 cells following treatment with various drugs ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( H ) The expression of CXCL12 in TAFs treated with different preparations ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( I-M ) The expression of CD206 and CD86 in vitro after RAW264.7 cells were treated with various drugs ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03663-w'>40826073</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-12951_2025_3663_fig9_html.pngAnti-Mannose Receptor/MRC1 Picoband® Antibody( A ) Protein expression images of α-SMA, CD206, and CXCR4 in tumors. ( B ) Quantitative analysis of α-SMA expression in tumors following drug treatment ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( C ) FM images and ( D ) quantitative analysis of α-SMA expression in tumors. Scale bar = 50 μm. ( n = 5, * p < 0.05, ** p < 0.01, *** p < 0.001). ( E ) Quantitative analysis of CD206 expression in tumors (n=3, * p <0.05, ** p <0.01, *** p <0.001). ( F ) FM images and ( G ) quantitative analysis of CD206 expression in tumors. Scale bar = 50 μm. ( n = 5, * p < 0.05, ** p < 0.01, *** p < 0.001). ( H ) Quantitative analysis of CXCR4 expression in tumors following treatment with various drugs ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001). ( I ) qPCR analysis of CXCL12 gene expression in tumors following treatment with various drugs ( n = 5, ** p < 0.01, *** p < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03663-w'>40826073</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-fbioe-13-1688905-g005.jpgAnti-Mannose Receptor/MRC1 Picoband® AntibodyHydrogel-induced macrophage M2 polarization in vitro . (A) Flow cytometry analysis of the macrophage surface marker CD206. (B) Immunofluorescence of CD206 in macrophages treated with different hydrogels. (C) Quantitative analysis of flow cytometry in CD206. (D) Mean fluorescence intensity of CD206 in macrophages treated with different hydrogels. (E, F) Quantitative analysis and immunofluorescence of CD163 in macrophages treated with different hydrogels.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1688905/full'>41190287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-fbioe-13-1688905-g007.jpgAnti-Mannose Receptor/MRC1 Picoband® AntibodyWound healing with different hydrogels in vivo . (A) Preparation and observation of diabetic chronic wounds related to orthopedics. (B) Photographs of the wound treated with the hydrogel in different hydrogel groups. (C) Wound recovery curve of different hydrogel groups. (D) H&E staining and Masson staining of wound tissue on day 10 in different hydrogel groups. (E) IHC staining of wound tissue in iNOS and CD206.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1688905/full'>41190287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-fphar-16-1649902-g009.jpgAnti-Mannose Receptor/MRC1 Picoband® AntibodyCS attenuates MMT in RIF rats. (A) Co-staining of the α-SMA (green) with CD68 (red) by Double Immunofluorescence. (B) Co-localization analysis of CD68+α-SMA + MMT cells. (C) Staining intensity of CD68+α-SMA + MMT cells was quantified. (D) Co-staining of the α-SMA (green) with CD206 (red) by Double Immunofluorescence. (E) Co-localization analysis of CD206+α-SMA + MMT cells. (F) Staining intensity of CD206+α-SMA + MMT cells was quantified (n = 4). (G) mRNA Expression of AKT1, EGFR, IL-6, and IL-10 in RIF rats kidney (n = 3). ## p < 0.01 vs. the sham group; * p < 0.05 vs. the model group; ** p < 0.01 vs. the model group. Scale bars (40 μm, 10 μm).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1649902/full'>41142245</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-ihc-testing-3.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-ihc-testing-4.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-ihc-testing-5.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IHC analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-if-testing-6.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IF analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-mrc1-primary-antibodies-if-testing-7.jpgAnti-Mannose Receptor/MRC1 Picoband® Antibody IF analysis of MRC1 using anti-MRC1 antibody (A02285-2). <br>
MRC1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MRC1 Antibody (A02285-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-13287_2021_2392_fig5_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyIL-1β-primed ERCs reduced macrophage infiltration and inflammatory cytokine expressions in colons. Intra-colon macrophage infiltration was evaluated by immunohistochemical staining. Specifically, we stained iNOS for detecting M1 cell infiltration, and CD206 for measuring M2 cell infiltration. The represent IHC images of mouse colons and quantitive data for cell counts of each group are shown in A (× 200) and B , respectively. The concentrations of inflammatory cytokine productions in colonic tissues were determined by ELISA kit and the relative mRNA expression changes were performed by real-time PCR. IFN-γ ( C , D ), IL-17 ( E , F ), TNF-α ( G , H ), IL-6 ( I , J ), IL-4 ( K , L ), and IL-10 ( M , N ) were shown, respectively, which are majorly secreted by Th1, Th2, Th17 cells, or CD206 + macrophages, and closely associated with the development of UC. Data were presented as mean ± standard deviation, and p values were calculated by using one-way ANOVA followed by the least significant difference (LSD) test ( n = 6, * p < 0.05, ** p < 0.01, *** p < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-021-02392-9'>34090510</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-13287_2021_2392_fig4_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyIL-1β-primed ERCs reduced the population of mature DCs, but increased M2 macrophages in colitis mice. To determine whether each treatment has an influence on regulating DC and macrophage phenotypes, anti-CD11c antibody and antigen presenting-related antibodies (anti-MHCII, anti-CD86) were used to measure mature DCs, while anti-CD68 antibody and anti-CD206 antibody were used for M2 phenotype macrophages in spleens. A Representative dot plots of CD11c + MHCII + DCs and CD11c + CD86 + DCs in spleens. B Dot plots of CD68 + CD206 + macrophages. C – E Percentage of CD11c + MHCII + DCs, CD11c + CD86 + DCs, and CD68 + CD206 + macrophages, respectively. Data were mean ± SD ( n = 6, * p < 0.05, ** p < 0.01, *** p < 0.001). p values were analyzed by one-way ANOVA followed by the LSD test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-021-02392-9'>34090510</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-fncel-16-874102-g003.jpgAnti-Mannose Receptor/MRC1 Picoband® AntibodyIdentification of the isolated polarized M2 microglia and exosomes. (A) The expression of M2 microglia markers Arg1 and CD206 detected by RT-PCR. (B) The expression of M2 microglia markers Arg1 and CD206 detected by Western blot analysis. (C) The expression of M2 microglia markers Arg1 and CD206 detected by immunofluorescence. (D) Images of M2-EXOs detected using TEM. (E) Measurements of particle sizes range of the isolated exosomes using NTA. (F) Detection of exosomal marker proteins CD63 and TSG101 using western blot. All experiments were repeated three times. *** P < 0.001 vs. control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096074/'>35573832</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-12951_2024_2494_fig9_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyHistological staining evaluation. FU (FA-UIO-66-NH 2 ), BFU (Bai@FA-UIO-66-NH 2 ). ( A ) H&E staining and ( B ) Safranin O staining of the knee joint of SD rats and ( C ) partial magnified detail (bar = 200 μm). ( D ) Immunofluorescence staining of iNOS/CD206/DAPI of the synovial of SD rats (scale bar = 200 μm) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02494-5'>38724958</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-12951_2024_2494_fig7_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyIn vitro anti-inflammatory effect produced by driving the polarization of macrophage M1 towards M2. FU (FA-UIO-66-NH 2 ), BFU (Bai@FA-UIO-66-NH 2 ). (A-G) In vitro mRNA expression of IL-1β, IL-6, iNOS, Arg-1, CD86, IL-10 and CD206 . H-J) Immunofluorescence staining of iNOS and CD206 after different treatments. (H) (scale bar = 100 μm) and Corresponding fluorescence intensity quantification (I-J). Flow cytometry analysis of specific marker of M1 macrophage iNOS (K) and M2 macrophage CD206 (L). ( n = 3, mean ± SD, “*” symbol compared with normal group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and “” symbol compared between groups, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02494-5'>38724958</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-43556_2024_203_fig6_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyMice reconstituted with FGF2 KO macrophages and subjected to CLP demonstrate increased M1 polarization in lung tissue. a - f The presence and levels of CD206, CD86, and F4/80 markers on macrophages within lung tissue were identified and quantitatively assessed using immunofluorescence staining. Bar is 20 μm. * p < 0.05, vs. WT; Δ p < 0.05 vs. WT + LPS; # p < 0.05 vs. FGF2 KO <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s43556-024-00203-0'>39436561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-43556_2024_203_fig2_html.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyEffect of FGF2 deficiency on BMDM apoptosis and polarization. a – c FGF2 deletion increased BMDM apoptosis. a Apoptosis in BMDM deprived of FBS for 24 h was assessed by flow cytometry ( n = 4). b - c Percentage of PI + Annexin V + and PI- Annexin V + BMDM after starvation. d - k FGF2 deletion in BMDM promoted M1 polarization. d - g Flow cytometric analysis of macrophage markers in BMDM treated with LPS or IL4, including CD86, iNOS, CD206, and Arg1 ( n = 3). h - k The levels of CD86, iNOS, CD206 and Arg1 in BMDM after treatment with LPS or IL4. N represents no treatment; * p < 0.05, vs. WT; Ψ p < 0.05, vs. N + WT; Ω p < 0.05, vs. N + FGF2 KO <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s43556-024-00203-0'>39436561</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-fphar-16-1430536-g006.jpgAnti-Mannose Receptor/MRC1 Picoband® AntibodyYNJ reduces the secretion of inflammatory factors and promotes M2 macrophage polarization. (A–C) ELISA of TNF-α (A) , IL-6 (B) , and IL-1β (C) contents in the supernatant of cultured RAW264.7 macrophages. (D) IF images of CD86 and CD206 detection in cultured RAW264.7 macrophages. (E, F) Statistical analysis of CD86 and CD206 expression by IF. The data are presented as the mean ± SD (n = 6). ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1430536/full'>39925847</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02285-2-13287_2022_2980_fig7_html_1.pngAnti-Mannose Receptor/MRC1 Picoband® AntibodyPF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-022-02980-3'>35941707</a></b>
https://www.bosterbio.com/products/primary-antibodies/anti-nd4-mtnd4-antibodya04180-2-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04180-2-mtnd4-primary-antibodies-wb-testing-1.jpgAnti-ND4/Mtnd4 Antibody Picoband® Western blot analysis of Mtnd4 using anti-Mtnd4 antibody (A04180-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mtnd4 antigen affinity purified polyclonal antibody (Catalog # A04180-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mtnd4 at approximately 48KD. The expected band size for Mtnd4 is at 52KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mta2-pid-picoband-trade-antibody-a03073-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-wb-testing-1.jpgAnti-MTA2/PID Picoband® Antibody Western blot analysis of MTA2 using anti-MTA2 antibody (A03073-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: rat liver tissue lysates, <br>
Lane 5: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # A03073-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA2 at approximately 75KD. The expected band size for MTA2 is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-ihc-testing-2.jpgAnti-MTA2/PID Picoband® Antibody IHC analysis of MTA2 using anti-MTA2 antibody (A03073-1). <br>
MTA2 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA2 Antibody (A03073-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-icc-testing-3.jpgAnti-MTA2/PID Picoband® Antibody IF analysis of MTA2 using anti-MTA2 antibody (A03073-1). <br>
MTA2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MTA2 Antibody (A03073-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-fc-testing-4.pngAnti-MTA2/PID Picoband® Antibody Flow Cytometry analysis of A431 cells using anti-MTA2 antibody (A03073-1). <br>Overlay histogram showing A431 cells stained with A03073-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTA2 Antibody (A03073-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-ihc-testing-5.jpgAnti-MTA2/PID Picoband® Antibody IHC analysis of MTA2 using anti-MTA2 antibody (A03073-1). <br>
MTA2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTA2 Antibody (A03073-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-ihc-testing-6.jpgAnti-MTA2/PID Picoband® Antibody IHC analysis of MTA2 using anti-MTA2 antibody (A03073-1). <br>
MTA2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTA2 Antibody (A03073-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-1-mta2-primary-antibodies-ihc-testing-7.jpgAnti-MTA2/PID Picoband® Antibody IHC analysis of MTA2 using anti-MTA2 antibody (A03073-1). <br>
MTA2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTA2 Antibody (A03073-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-xlf-nhej1-picoband-trade-antibody-a03552-3-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-wb-testing-1.jpgAnti-XLF/NHEJ1 Picoband® Antibody Western blot analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHEJ1 antigen affinity purified polyclonal antibody (Catalog # A03552-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHEJ1 at approximately 38KD. The expected band size for NHEJ1 is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-ihc-testing-2.jpgAnti-XLF/NHEJ1 Picoband® Antibody IHC analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). <br>
NHEJ1 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NHEJ1 Antibody (A03552-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-ihc-testing-3.jpgAnti-XLF/NHEJ1 Picoband® Antibody IHC analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). <br>
NHEJ1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NHEJ1 Antibody (A03552-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-icc-testing-4.jpgAnti-XLF/NHEJ1 Picoband® Antibody IF analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). <br>
NHEJ1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NHEJ1 Antibody (A03552-3) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-icc-testing-5.jpgAnti-XLF/NHEJ1 Picoband® Antibody IF analysis of NHEJ1 using anti-NHEJ1 antibody (A03552-3). <br>
NHEJ1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NHEJ1 Antibody (A03552-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-fc-testing-6.pngAnti-XLF/NHEJ1 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-NHEJ1 antibody (A03552-3). <br>Overlay histogram showing HEPA1-6 cells stained with A03552-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NHEJ1 Antibody (A03552-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03552-3-nhej1-primary-antibodies-fc-testing-7.pngAnti-XLF/NHEJ1 Picoband® Antibody Flow Cytometry analysis of K562 cells using anti-NHEJ1 antibody (A03552-3). <br>Overlay histogram showing K562 cells stained with A03552-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NHEJ1 Antibody (A03552-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-niemann-pick-c2-npc2-picoband-trade-antibody-a01582-3-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-3-npc2-primary-antibodies-wb-testing-1_1.jpgAnti-Niemann Pick C2/NPC2 Picoband® Antibody Western blot analysis of NPC2 using anti-NPC2 antibody (A01582-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPC2 antigen affinity purified polyclonal antibody (Catalog # A01582-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPC2 at approximately 19 kDa. The expected band size for NPC2 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-3-npc2-primary-antibodies-ihc-testing-2.jpgAnti-Niemann Pick C2/NPC2 Picoband® Antibody IHC analysis of NPC2 using anti-NPC2 antibody (A01582-3). <br>
NPC2 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NPC2 Antibody (A01582-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-3-npc2-primary-antibodies-ihc-testing-3.jpgAnti-Niemann Pick C2/NPC2 Picoband® Antibody IHC analysis of NPC2 using anti-NPC2 antibody (A01582-3). <br>
NPC2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NPC2 Antibody (A01582-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-3-npc2-primary-antibodies-fc-testing-4.pngAnti-Niemann Pick C2/NPC2 Picoband® Antibody Flow Cytometry analysis of HepG2 cells using anti-NPC2 antibody (A01582-3). <br>Overlay histogram showing HepG2 cells stained with A01582-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NPC2 Antibody (A01582-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01582-3-npc2-primary-antibodies-if-testing-5.jpgAnti-Niemann Pick C2/NPC2 Picoband® Antibody IF analysis of NPC2 using anti-NPC2 antibody (A01582-3). <br>
NPC2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NPC2 Antibody (A01582-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-natriuretic-peptide-receptor-a-gc-a-npr1-picoband-trade-antibody-a01042-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01042-npr1-primary-antibodies-wb-testing-1.jpgAnti-Natriuretic Peptide Receptor A/GC-A/NPR1 Picoband® Antibody Western blot analysis of NPR1 using anti-NPR1 antibody (A01042).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPR1 antigen affinity purified polyclonal antibody (Catalog # A01042) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPR1 at approximately 119KD. The expected band size for NPR1 is at 119KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01042-npr1-primary-antibodies-icc-testing-2.jpgAnti-Natriuretic Peptide Receptor A/GC-A/NPR1 Picoband® Antibody IF analysis of NPR1 using anti-NPR1 antibody (A01042). <br>
NPR1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NPR1 Antibody (A01042) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-paf1-pd2-picoband-trade-antibody-a01640-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01640-1-paf1-primary-antibodies-wb-testing-1.jpgAnti-PAF1/PD2 Picoband® Antibody Western blot analysis of PAF1 using anti-PAF1 antibody (A01640-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human A431 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: human SW620 whole cell lysates, <br>
Lane 5: rat heart tissue lysates, <br>
Lane 6: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAF1 antigen affinity purified polyclonal antibody (Catalog # A01640-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAF1 at approximately 80KD. The expected band size for PAF1 is at 60KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01640-1-paf1-primary-antibodies-icc-testing-2.jpgAnti-PAF1/PD2 Picoband® Antibody IF analysis of PAF1 using anti-PAF1 antibody (A01640-1). <br>
PAF1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PAF1 Antibody (A01640-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-pannexin-2-panx2-picoband-trade-antibody-a08860-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08860-1-panx2-primary-antibodies-wb-testing-1.jpgAnti-Pannexin 2/PANX2 Picoband® Antibody Western blot analysis of PANX2 using anti-PANX2 antibody (A08860-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: humna SW579 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PANX2 antigen affinity purified polyclonal antibody (Catalog # A08860-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PANX2 at approximately 74KD. The expected band size for PANX2 is at 74KD.
https://www.bosterbio.com/products/primary-antibodies/anti-scop-phlpp1-picoband-trade-antibody-a02430-1-boster.html2026-03-24T05:26:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02430-1-phlpp1-primary-antibodies-wb-testing-1.jpgAnti-SCOP/PHLPP1 Picoband® Antibody Western blot analysis of PHLPP1 using anti-PHLPP1 antibody (A02430-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human A375 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLPP1 antigen affinity purified polyclonal antibody (Catalog # A02430-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHLPP1 at approximately 140KD. The expected band size for PHLPP1 is at 185KD.
https://www.bosterbio.com/products/primary-antibodies/anti-picalm-picoband-trade-antibody-a02053-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02053-1-picalm-primary-antibodies-wb-testing-1.jpgAnti-PICALM Picoband® Antibody Western blot analysis of PICALM using anti-PICALM antibody (A02053-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: rat lung tissue lysates, <br>
Lane 3: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PICALM antigen affinity purified polyclonal antibody (Catalog # A02053-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PICALM at approximately 71KD. The expected band size for PICALM is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02053-1-picalm-primary-antibodies-ihc-testing-2.jpgAnti-PICALM Picoband® Antibody IHC analysis of PICALM using anti-PICALM antibody (A02053-1). <br>
PICALM was detected in paraffin-embedded section of human Ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PICALM Antibody (A02053-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02053-1-picalm-primary-antibodies-icc-testing-3.jpgAnti-PICALM Picoband® Antibody IF analysis of PICALM using anti-PICALM antibody (A02053-1). <br>
PICALM was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PICALM Antibody (A02053-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02053-1-picalm-primary-antibodies-fc-testing-4.pngAnti-PICALM Picoband® Antibody Flow Cytometry analysis of A431 cells using anti-PICALM antibody (A02053-1). <br>Overlay histogram showing A431 cells stained with A02053-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PICALM Antibody (A02053-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-polymeric-immunoglobulin-receptor-pigr-picoband-trade-antibody-a01624-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01624-pigr-primary-antibodies-wb-testing-1_1.jpgAnti-Polymeric immunoglobulin receptor/PIGR Picoband® Antibody Western blot analysis of PIGR using anti-PIGR antibody (A01624). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates,<br>
Lane 2: mouse liver tissue lysates.<br>
Lane 3: mouse intestine tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody (Catalog # A01624) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGR at approximately 85-100 kDa. The expected band size for PIGR is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01624-pigr-primary-antibodies-fcm-testing-2.jpgAnti-Polymeric immunoglobulin receptor/PIGR Picoband® Antibody Flow Cytometry analysis of RH35 cells using anti-PIGR antibody (A01624). <br>Overlay histogram showing RH35 cells stained with A01624 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGR Antibody (A01624, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pld2-picoband-trade-antibody-a02586-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02586-pld2-primary-antibodies-wb-testing-1.jpgAnti-PLD2 Picoband® Antibody Western blot analysis of PLD2 using anti-PLD2 antibody (A02586).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A375 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: rat brain tissue lysates, <br>
Lane 4: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD2 antigen affinity purified polyclonal antibody (Catalog # A02586) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD2 at approximately 106KD. The expected band size for PLD2 is at 95KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02586-pld2-primary-antibodies-icc-testing-2.jpgAnti-PLD2 Picoband® Antibody IF analysis of PLD2 using anti-PLD2 antibody (A02586). <br>
PLD2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PLD2 Antibody (A02586) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02586-pld2-primary-antibodies-fc-testing-3.pngAnti-PLD2 Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-PLD2 antibody (A02586). <br>Overlay histogram showing U937 cells stained with A02586 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLD2 Antibody (A02586, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-melanoma-gp100-pmel-picoband-trade-antibody-a01262-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01262-2-pmel-primary-antibodies-wb-testing-1.jpgAnti-Melanoma gp100/Pmel Picoband® Antibody Western blot analysis of Pmel using anti-Pmel antibody (A01262-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat C6 whole cell lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pmel antigen affinity purified polyclonal antibody (Catalog # A01262-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pmel at approximately 95KD. The expected band size for Pmel is at 70KD.
https://www.bosterbio.com/products/primary-antibodies/anti-pgc1-beta-ppargc1b-picoband-trade-antibody-a02933-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02933-1-ppargc1b-primary-antibodies-wb-testing-1.jpgAnti-PGC1 beta/PPARGC1B Picoband® Antibody Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (A02933-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Caco-2 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates. <br>
Lane 4: human MDA-MB-453 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified polyclonal antibody (Catalog # A02933-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113KD. The expected band size for PPARGC1B is at 113KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02933-1-ppargc1b-primary-antibodies-fc-testing-2.pngAnti-PGC1 beta/PPARGC1B Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-PPARGC1B antibody (A02933-1). <br>Overlay histogram showing U937 cells stained with A02933-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPARGC1B Antibody (A02933-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02933-1-ppargc1b-primary-antibodies-fc-testing-3.pngAnti-PGC1 beta/PPARGC1B Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-PPARGC1B antibody (A02933-1). <br>Overlay histogram showing HEPA1-6 cells stained with A02933-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPARGC1B Antibody (A02933-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ppm1a-picoband-trade-antibody-a02928-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-wb-testing-1.jpgAnti-PPM1A Picoband® AntibodyWestern blot analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1A antigen affinity purified polyclonal antibody (A02928-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPM1A at approximately 22 kDa. The expected band size for PPM1A is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-ihc-testing-2.jpgAnti-PPM1A Picoband® Antibody IHC analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>
PPM1A was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPM1A Antibody (A02928-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-ihc-testing-3.jpgAnti-PPM1A Picoband® Antibody IHC analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>
PPM1A was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPM1A Antibody (A02928-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-ihc-testing-4.jpgAnti-PPM1A Picoband® Antibody IHC analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>
PPM1A was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPM1A Antibody (A02928-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-ihc-testing-5.jpgAnti-PPM1A Picoband® Antibody IHC analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>
PPM1A was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPM1A Antibody (A02928-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02928-1-ppm1a-primary-antibodies-if-testing-1.jpgAnti-PPM1A Picoband® AntibodyIF analysis of PPM1A using anti-PPM1A antibody (A02928-1). <br>
PPM1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1A Antibody (A02928-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-ppm1d-wip1-picoband-trade-antibody-a01576-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01576-1-ppm1d-primary-antibodies-wb-testing-1_1.jpgAnti-PPM1D/WIP1 Picoband® Antibody Western blot analysis of PPM1D using anti-PPM1D antibody (A01576-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human MDA-MB-453 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human 293T whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse kidney tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1D antigen affinity purified polyclonal antibody (Catalog # A01576-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1D at approximately 67 kDa. The expected band size for PPM1D is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01576-1-ppm1d-primary-antibodies-fc-testing-3.pngAnti-PPM1D/WIP1 Picoband® Antibody Flow Cytometry analysis of A431 cells using anti-PPM1D antibody (A01576-1). <br>Overlay histogram showing A431 cells stained with A01576-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1D Antibody (A01576-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ppm1e-picoband-trade-antibody-a08794-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08794-ppm1e-primary-antibodies-wb-testing-1.jpgAnti-PPM1E Picoband® Antibody Western blot analysis of PPM1E using anti-PPM1E antibody (A08794).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1E antigen affinity purified polyclonal antibody (Catalog # A08794) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1E at approximately 84KD. The expected band size for PPM1E is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08794-ppm1e-primary-antibodies-ihc-testing-2.jpgAnti-PPM1E Picoband® Antibody IHC analysis of PPM1E using anti-PPM1E antibody (A08794). <br>
PPM1E was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPM1E Antibody (A08794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08794-ppm1e-primary-antibodies-icc-testing-3.jpgAnti-PPM1E Picoband® Antibody IF analysis of PPM1E using anti-PPM1E antibody (A08794). <br>
PPM1E was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PPM1E Antibody (A08794) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08794-ppm1e-primary-antibodies-fc-testing-4.pngAnti-PPM1E Picoband® Antibody Flow Cytometry analysis of K562 cells using anti-PPM1E antibody (A08794). <br>Overlay histogram showing K562 cells stained with A08794 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1E Antibody (A08794, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-prame-picoband-trade-antibody-a06628-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06628-2-prame-primary-antibodies-wb-testing-1.jpgAnti-PRAME Picoband® Antibody Western blot analysis of PRAME using anti-PRAME antibody (A06628-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAME antigen affinity purified polyclonal antibody (Catalog # A06628-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAME at approximately 58KD. The expected band size for PRAME is at 58KD.
https://www.bosterbio.com/products/primary-antibodies/anti-cox2-cyclooxygenase-2-ptgs2-picoband-trade-antibody-a00084-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-cox2-primary-antibodies-wb-testing-1.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: mouse RAW264.7(-LPS) whole cell lysates, <br>
Lane 3: mouse RAW264.7(+LPS) whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-cox2-primary-antibodies-ihc-testing-2.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). <br>
PTGS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTGS2 Antibody (A00084-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-41598_2018_34156_fig4_html.pngAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® AntibodyEffects of maltol on the levels of inflammation cytokines in cisplatin-induced renal toxicity. ( A ) Effects of maltol on the positive expressions of Bax, Bcl-2, iNOS and COX-2 in renal tissues were examined by IHC in renal tissues (magnification × 200), And the column chart shows stained area, semiquantitative analysis of Bax, Bcl-2, iNOS and COX-2 expression in kidneys to IHC. ( B ) Inflammation cytokines level of TNF-α, IL-1β, iNOS and NF-κB in serum of mice were measured by ELISA kits. All values were expressed as mean ± S.D. * p < 0.05, ** p < 0.01 vs . normal group; # p < 0.05, ## p < 0.01 vs . cisplatin group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-34156-6'>30374107</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-feb413914-fig-0002-m.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® AntibodyAntioxidant and anti-inflammatory effects of OVE in LPS-stimulated RAW264.7 cells. (A, B) Cell viability measured by CCK8 assay. (C) Analysis of ROS levels detected by DCFH-DA probe. (D) Quantitative analysis of gene expression levels of iNos, Il6, Il-1b, Tnfα, and Cox-2 by qRT-PCR. (E) NO production analysis by NO assay. (F) ELISA results of IL6, IL-1β, and TNFα. (G) Protein expression levels of COX-2, IL6, IL-1β, and TNFα. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ####P < 0.0001, compared to the untreated control group; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, compared to the LPS control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/2211-5463.13914'>39455284</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-cox2-primary-antibodies-ihc-testing-3.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody IHC analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). <br>
PTGS2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTGS2 Antibody (A00084-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-ptgs2-primary-antibodies-fc-testing-4_1.pngAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody Flow Cytometry analysis of CACO-2 cells using anti-PTGS2 antibody (A00084-2). <br>
Overlay histogram showing CACO-2 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00084-2-ptgs2-primary-antibodies-fc-testing-5_1.pngAnti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-PTGS2 antibody (A00084-2). <br>
Overlay histogram showing HEPA1-6 cells stained with A00084-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGS2 Antibody (A00084-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-ptpn22-picoband-trade-antibody-a00581-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00581-2-ptpn22-primary-antibodies-wb-testing-1.jpgAnti-PTPN22 Picoband® Antibody Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: rat lung tissue lysates, <br>
Lane 4: rat PC-12 whole cell lysates, <br>
Lane 5: mouse lung tissue lysates, <br>
Lane 6: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 100KD. The expected band size for PTPN22 is at 92KD.
https://www.bosterbio.com/products/primary-antibodies/anti-rbpjk-rbpj-picoband-trade-antibody-a00767-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00767-1-rbpj-primary-antibodies-wb-testing-1.jpgAnti-RBPJK/RBPJ Picoband® Antibody Western blot analysis of RBPJ using anti-RBPJ antibody (A00767-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: rat NRK whole cell lysates, <br>
Lane 3: mouse NIH3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPJ antigen affinity purified polyclonal antibody (Catalog # A00767-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPJ at approximately 60KD. The expected band size for RBPJ is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00767-1-41467_2022_33836_fig8_html.pngAnti-RBPJK/RBPJ Picoband® AntibodyNotch activity promotes microglial development in vivo and in vitro. a , b Immunofluorescence ( a ) and fluorescent intensity emission values ( b ) of RBPJ (red) in F4/80 + (green) cells in the sagittal sections of E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 3 mice in each group). Each dot in ( b ) denotes one cell. Ten cells per mouse were quantified. The white arrow indicates the co-localized signals of RBPJ and F4/80, whereas white arrowhead point to the limited signals of RBPJ. c , d Immunofluorescence ( c ) and quantified cell density ( d ) of IBA1 + cells in the sagittal sections of E14.5 Rbpj fl/fl , Cx3cr1 cre/+ and Cx3cr1 cre/+ Rbpj fl/fl mice ( n = 6 mice in each group). Each dot denotes one mouse. Three slices per mouse were quantified. e Transcriptional levels of Sall1 , Tgfb1 , Fcrls , Slc2a5 , and Gpr34 in CD11b hi CD45 lo cells from E14.5 Rbpj fl/fl and Cx3cr1 cre/+ Rbpj fl/fl mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of IBA1 and Hes1 in the embryonic microglia in vitro furnished with MCSF or additional DLL3 for 5 days. g Quantification of IBA1 + cell density in ( f ). Data were pooled from three independent experiments. Cultured IBA1 + cells in each experiment were from twenty E12.5 mice mesencephalon. Each dot represents individual experiment. h The percentage of Hes1 - IBA1 + or Hes1 + IBA1 + cells after cultured 5 days in ( f ). The number in each histogram indicates the average percentage. Data are pooled from three independent experiments. i Transcriptional levels of Hes1, Mcm5, Dab2 , Pros1 , Tmem119 , Gpr34 , and Sall1 in embryonic microglia after cultured for 5 days. The data were from three independent experiments. Each dot represents an independent experiment. j Schematic diagram of Notch activation in mouse microglial differentiation, created with BioRender.com. Error bars, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns no significant, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-33836-2'>36253375</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00767-1-rbpj-primary-antibodies-fc-testing-2.pngAnti-RBPJK/RBPJ Picoband® Antibody Flow Cytometry analysis of HL-60 cells using anti-RBPJ antibody (A00767-1). <br>Overlay histogram showing HL-60 cells stained with A00767-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBPJ Antibody (A00767-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00767-1-rbpj-primary-antibodies-if-testing-3.jpgAnti-RBPJK/RBPJ Picoband® Antibody IF analysis of RBPJK/RBPJ using anti-RBPJK/RBPJ antibody (A00767-1). <br>
RBPJK/RBPJ was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RBPJK/RBPJ Antibody (A00767-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-rnf168-picoband-trade-antibody-a01224-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01224-1-rnf168-primary-antibodies-wb-testing-1.jpgAnti-RNF168 Picoband® Antibody Western blot analysis of RNF168 using anti-RNF168 antibody (A01224-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF168 antigen affinity purified polyclonal antibody (Catalog # A01224-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF168 at approximately 65KD. The expected band size for RNF168 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01224-1-rnf168-primary-antibodies-ihc-testing-2.jpgAnti-RNF168 Picoband® Antibody IHC analysis of RNF168 using anti-RNF168 antibody (A01224-1). <br>
RNF168 was detected in paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNF168 Antibody (A01224-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01224-1-rnf168-primary-antibodies-icc-testing-3.jpgAnti-RNF168 Picoband® Antibody IF analysis of RNF168 using anti-RNF168 antibody (A01224-1). <br>
RNF168 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RNF168 Antibody (A01224-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01224-1-rnf168-primary-antibodies-fc-testing-4.pngAnti-RNF168 Picoband® Antibody Flow Cytometry analysis of CACO-2 cells using anti-RNF168 antibody (A01224-1). <br>Overlay histogram showing CACO-2 cells stained with A01224-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF168 Antibody (A01224-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rsk-2-mapkap-kinase-1b-rps6ka3-picoband-trade-antibody-a02215-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02215-2-rps6ka3-primary-antibodies-wb-testing-1_1.jpgAnti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human CACO-2 whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human A549 whole cell lysates,<br>
Lane 5: rat skeletal muscle tissue lysates,<br>
Lane 6: rat thymus tissue lysates,<br>
Lane 7: mouse skeletal muscle tissue lysates,<br>
Lane 8: mouse thymus tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02215-2-rps6ka3-primary-antibodies-ip-testing-3.jpgAnti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody Immunoprecipitating RPS6KA3 in A549 whole cell lysate.<br>Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2).<br>Lane 1: A549 whole cell lysates (30ug)<br>Lane 2: Rabbit control IgG instead of anti-RPS6KA3 antibody in A549 whole cell lysate.<br>Lane 3: anti-RPS6KA3 antibody (2μg) + A549 whole cell lysate (500μg)<br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (A02215-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02215-2-rps6ka3-primary-antibodies-if-testing-2.jpgAnti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody IF analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2) and anti-Beta Tubulin antibody (M01857-3).<br>
RPS6KA3 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS6KA3 Antibody (A02215-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02215-2-rps6ka3-primary-antibodies-fcm-testing-4.jpgAnti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody Flow Cytometry analysis of 293T cells using anti-RPS6KA3 antibody (A02215-2). <br>
Overlay histogram showing 293T cells stained with A02215-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS6KA3 Antibody (A02215-2, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-senp6-picoband-trade-antibody-a05088-3-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05088-3-senp6-primary-antibodies-wb-testing-1.jpgAnti-SENP6 Picoband® Antibody Western blot analysis of SENP6 using anti-SENP6 antibody (A05088-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP6 antigen affinity purified polyclonal antibody (Catalog # A05088-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP6 at approximately 150KD. The expected band size for SENP6 is at 126KD.
https://www.bosterbio.com/products/primary-antibodies/anti-clld8-setdb2-picoband-trade-antibody-a08290-2-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08290-2-setdb2-primary-antibodies-wb-testing-1.jpgAnti-CLLD8/SETDB2 Picoband® Antibody Western blot analysis of SETDB2 using anti-SETDB2 antibody (A08290-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human THP-1 whole cell lysates, <br>
Lane 2: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETDB2 antigen affinity purified polyclonal antibody (Catalog # A08290-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETDB2 at approximately 82KD. The expected band size for SETDB2 is at 82KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08290-2-setdb2-primary-antibodies-fc-testing-2.pngAnti-CLLD8/SETDB2 Picoband® Antibody Flow Cytometry analysis of THP-1 cells using anti-SETDB2 antibody (A07692-1). <br>Overlay histogram showing THP-1 cells stained with A07692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETDB2 Antibody (A07692-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-eaat1-slc1a3-picoband-trade-antibody-a02133-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-slc1a3-primary-antibodies-wb-testing-1.jpgAnti-EAAT1/SLC1A3 Picoband® Antibody Western blot analysis of SLC1A3 using anti-SLC1A3 antibody (A02133).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC1A3 antigen affinity purified polyclonal antibody (Catalog # A02133) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC1A3 at approximately 60KD. The expected band size for SLC1A3 is at 60KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-slc1a3-primary-antibodies-fc-testing-2.pngAnti-EAAT1/SLC1A3 Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-SLC1A3 antibody (A02133). <br>Overlay histogram showing U937 cells stained with A02133 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC1A3 Antibody (A02133, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-slc25a1-picoband-trade-antibody-a05995-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05995-1-slc25a1-primary-antibodies-wb-testing-1.jpgAnti-Slc25a1 Picoband® Antibody Western blot analysis of SLC25A1 using anti-SLC25A1 antibody (A05995-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human CCRF-CEM whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A1 antigen affinity purified polyclonal antibody (Catalog # A05995-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A1 at approximately 30-34KD. The expected band size for SLC25A1 is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05995-1-slc25a1-primary-antibodies-icc-testing-2.jpgAnti-Slc25a1 Picoband® Antibody IF analysis of SLC25A1 using anti-SLC25A1 antibody (A05995-1). <br>
SLC25A1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC25A1 Antibody (A05995-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-sorbs1-picoband-trade-antibody-a04426-3-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04426-3-sorbs1-primary-antibodies-wb-testing-1_1.jpgAnti-SORBS1 Picoband® Antibody Western blot analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS1 antigen affinity purified polyclonal antibody (A04426-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SORBS1 at approximately 160 kDa. The expected band size for SORBS1 is at 143 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04426-3-sorbs1-primary-antibodies-ihc-testing-2.jpgAnti-SORBS1 Picoband® Antibody IHC analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). <br>
SORBS1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS1 Antibody (A04426-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04426-3-sorbs1-primary-antibodies-fc-testing-3.pngAnti-SORBS1 Picoband® Antibody Flow Cytometry analysis of PC-3 cells using anti-SORBS1 antibody (A04426-3). <br>Overlay histogram showing PC-3 cells stained with A04426-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SORBS1 Antibody (A04426-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sufu-picoband-trade-antibody-a02279-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02279-1-sufu-primary-antibodies-wb-testing-1_1.jpgAnti-SUFU Picoband® Antibody Western blot analysis of SUFU using anti-SUFU antibody (A02279-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human 293T whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUFU antigen affinity purified polyclonal antibody (Catalog # A02279-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUFU at approximately 54 kDa. The expected band size for SUFU is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02279-1-sufu-primary-antibodies-icc-testing-2.jpgAnti-SUFU Picoband® Antibody IF analysis of SUFU using anti-SUFU antibody (A02279-1). <br>
SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02279-1-sufu-primary-antibodies-fc-testing-3.pngAnti-SUFU Picoband® Antibody Flow Cytometry analysis of U937 cells using anti-SUFU antibody (A02279-1). <br>Overlay histogram showing U937 cells stained with A02279-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUFU Antibody (A02279-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-synaptophysin-syp-picoband-trade-antibody-a05049-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-wb-testing-1.jpgAnti-Synaptophysin/SYP Picoband® Antibody Western blot analysis of SYP using anti-SYP antibody (A05049).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP antigen affinity purified polyclonal antibody (Catalog # A05049) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-if-testing-2.jpgAnti-Synaptophysin/SYP Picoband® Antibody IF analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SYP Antibody (A05049) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-3.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-4.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-5.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-6.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-7.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-ihc-testing-8.jpgAnti-Synaptophysin/SYP Picoband® Antibody IHC analysis of SYP using anti-SYP antibody (A05049). <br>
SYP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYP Antibody (A05049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-if-testing-9.jpgAnti-Synaptophysin/SYP Picoband® Antibody IF analysis of SYP using anti-SYP antibody (PB9426). <br>
SYP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SYP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-if-testing-10.jpgAnti-Synaptophysin/SYP Picoband® Antibody IF analysis of SYP using anti-SYP antibody (PB9426). <br>
SYP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SYP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05049-syp-primary-antibodies-if-testing-11.jpgAnti-Synaptophysin/SYP Picoband® Antibody IF analysis of SYP using anti-SYP antibody (PB9426). <br>
SYP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SYP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-t-bet-tbx21-antibodya00404-1-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00404-1-tbx21-primary-antibodies-wb-testing-1.jpgAnti-T-bet/Tbx21 Antibody Picoband® Western blot analysis of TBX21 using anti-TBX21 antibody (A00404-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: mouse RAW246.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX21 antigen affinity purified polyclonal antibody (Catalog # A00404-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX21 at approximately 65KD. The expected band size for TBX21 is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00404-1-tbx21-primary-antibodies-fc-testing-2.pngAnti-T-bet/Tbx21 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-TBX21 antibody (A00404-1). <br>Overlay histogram showing HEPA1-6 cells stained with A00404-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX21 Antibody (A00404-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00404-1-tbx21-primary-antibodies-fc-testing-3.pngAnti-T-bet/Tbx21 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-TBX21 antibody (A00404-1). <br>Overlay histogram showing NRK cells stained with A00404-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX21 Antibody (A00404-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00404-1-tbx21-primary-antibodies-fc-testing-4.pngAnti-T-bet/Tbx21 Antibody Picoband® Flow Cytometry analysis of mouse PBMC cells using anti-TBX21 antibody (A00404-1). <br>Overlay histogram showing mouse PBMC cells stained with A00404-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX21 Antibody (A00404-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rank-tnfrsf11a-picoband-trade-antibody-a01064-3-boster.html2026-03-24T05:26:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01064-3-tnfrsf11a-primary-antibodies-wb-testing-1.jpgAnti-RANK/Tnfrsf11a Picoband® Antibody Western blot analysis of Tnfrsf11a using anti-Tnfrsf11a antibody (A01064-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: mouse thymus tissue lysates, <br>
Lane 3: mouse RAW264.7 whole cell lysates, <br>
Lane 4: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf11a antigen affinity purified polyclonal antibody (Catalog # A01064-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf11a at approximately 80KD. The expected band size for Tnfrsf11a is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01064-3-tnfrsf11a-primary-antibodies-fc-testing-2.pngAnti-RANK/Tnfrsf11a Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-Tnfrsf11a antibody (A01064-3). <br>
Overlay histogram showing HEPA1-6 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01064-3-tnfrsf11a-primary-antibodies-fc-testing-3.pngAnti-RANK/Tnfrsf11a Picoband® Antibody Flow Cytometry analysis of RH-35 cells using anti-Tnfrsf11a antibody (A01064-3). <br>
Overlay histogram showing RH-35 cells stained with A01064-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf11a Antibody (A01064-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd30-tnfrsf8-picoband-trade-antibody-a01225-1-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01225-1-tnfrsf8-primary-antibodies-wb-testing-1.jpgAnti-CD30/Tnfrsf8 Picoband® Antibody Western blot analysis of Tnfrsf8 using anti-Tnfrsf8 antibody (A01225-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: mouse thymus tissue lysates, <br>
Lane 4: mouse RAW264.7 whole cell lysates, <br>
Lane 5: mouse SP20 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf8 antigen affinity purified polyclonal antibody (Catalog # A01225-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf8 at approximately 65KD. The expected band size for Tnfrsf8 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01225-1-tnfrsf8-primary-antibodies-fc-testing-2.pngAnti-CD30/Tnfrsf8 Picoband® Antibody Flow Cytometry analysis of HEPA1-6 cells using anti-Tnfrsf8 antibody (A01225-1). <br>Overlay histogram showing HEPA1-6 cells stained with A01225-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf8 Antibody (A01225-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01225-1-tnfrsf8-primary-antibodies-fc-testing-3.pngAnti-CD30/Tnfrsf8 Picoband® Antibody Flow Cytometry analysis of NRK cells using anti-Tnfrsf8 antibody (A01225-1). <br>Overlay histogram showing NRK cells stained with A01225-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tnfrsf8 Antibody (A01225-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gitr-tnfrsf18-picoband-trade-antibody-a03125-3-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03125-3-tnfrsf18-primary-antibodies-wb-testing-1.jpgAnti-GITR/Tnfrsf18 Picoband® Antibody Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody (A03125-3).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: rat skeletal muscle tissue lysates, <br>
Lane 3: mouse skeletal muscle tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf18 antigen affinity purified polyclonal antibody (Catalog # A03125-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf18 at approximately 30-35KD. The expected band size for Tnfrsf18 is at 26KD.
https://www.bosterbio.com/products/primary-antibodies/anti-fat10-ubd-picoband-trade-antibody-a01970-1-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01970-1-ubd-primary-antibodies-wb-testing-1.jpgAnti-FAT10/UBD Picoband® Antibody Western blot analysis of UBD using anti-UBD antibody (A01970-1).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBD antigen affinity purified polyclonal antibody (Catalog # A01970-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBD at approximately 18KD. The expected band size for UBD is at 18KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ube3a-picoband-trade-antibody-a00582-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-wb-testing-1_1.jpgAnti-UBE3A Picoband® Antibody Western blot analysis of UBE3A using anti-UBE3A antibody (A00582). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human HepG2 whole cell lysates,<br>
Lane 5: human Hela whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE3A antigen affinity purified polyclonal antibody (Catalog # A00582) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE3A at approximately 100 kDa. The expected band size for UBE3A is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-ihc-testing-4.jpgAnti-UBE3A Picoband® AntibodyIHC analysis of UBE3A using anti-UBE3A antibody (A00582). <br>UBE3A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE3A Antibody (A00582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-ihc-testing-5.jpgAnti-UBE3A Picoband® AntibodyIHC analysis of UBE3A using anti-UBE3A antibody (A00582). <br>UBE3A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE3A Antibody (A00582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-ihc-testing-6.jpgAnti-UBE3A Picoband® AntibodyIHC analysis of UBE3A using anti-UBE3A antibody (A00582). <br>UBE3A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE3A Antibody (A00582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-ihc-testing-2.jpgAnti-UBE3A Picoband® Antibody IHC analysis of UBE3A using anti-UBE3A antibody (A00582). <br>
UBE3A was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE3A Antibody (A00582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-ihc-testing-3.jpgAnti-UBE3A Picoband® Antibody IHC analysis of UBE3A using anti-UBE3A antibody (A00582). <br>
UBE3A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBE3A Antibody (A00582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00582-ube3a-primary-antibodies-fc-testing-4.pngAnti-UBE3A Picoband® Antibody Flow Cytometry analysis of CACO-2 cells using anti-UBE3A antibody (A00582). <br>Overlay histogram showing CACO-2 cells stained with A00582 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE3A Antibody (A00582, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ubr2-picoband-trade-antibody-a05812-2-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-wb-testing-1.jpgAnti-UBR2 Picoband® Antibody Western blot analysis of UBR2 using anti-UBR2 antibody (A05812-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: mouse RAW264.7 tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBR2 antigen affinity purified polyclonal antibody (Catalog # A05812-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 201KD. The expected band size for UBR2 is at 201KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-ihc-testing-2.jpgAnti-UBR2 Picoband® Antibody IHC analysis of UBR2 using anti-UBR2 antibody (A05812-2). <br>
UBR2 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBR2 Antibody (A05812-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-ihc-testing-3.jpgAnti-UBR2 Picoband® Antibody IHC analysis of UBR2 using anti-UBR2 antibody (A05812-2). <br>
UBR2 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBR2 Antibody (A05812-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-ihc-testing-4.jpgAnti-UBR2 Picoband® Antibody IHC analysis of UBR2 using anti-UBR2 antibody (A05812-2). <br>
UBR2 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UBR2 Antibody (A05812-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-icc-testing-5.jpgAnti-UBR2 Picoband® Antibody IF analysis of UBR2 using anti-UBR2 antibody (A05812-2). <br>
UBR2 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UBR2 Antibody (A05812-2) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05812-2-ubr2-primary-antibodies-fc-testing-6.pngAnti-UBR2 Picoband® Antibody Flow Cytometry analysis of HepG2 cells using anti-UBR2 antibody (A05812-2). <br>Overlay histogram showing HepG2 cells stained with A05812-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBR2 Antibody (A05812-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-vegf-vegfa-picoband-trade-antibody-a00045-2-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00045-2-vegfa-primary-antibodies-wb-testing-1.jpgAnti-VEGF/Vegfa Picoband® Antibody Western blot analysis of Vegfa using anti-Vegfa antibody (A00045-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfa antigen affinity purified polyclonal antibody (Catalog # A00045-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfa at approximately 27KD. The expected band size for Vegfa is at 25KD.
https://www.bosterbio.com/products/primary-antibodies/anti-wnt10a-picoband-trade-antibody-a03479-2-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03479-2-wnt10a-primary-antibodies-wb-testing-1.jpgAnti-Wnt10a Picoband® Antibody Western blot analysis of WNT10A using anti-WNT10A antibody (A03479-2).<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10A antigen affinity purified polyclonal antibody (Catalog # A03479-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10A at approximately 46KD. The expected band size for WNT10A is at 46KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03479-2-wnt10a-primary-antibodies-fc-testing-2.pngAnti-Wnt10a Picoband® Antibody Flow Cytometry analysis of A431 cells using anti-WNT10A antibody (A03479-2). <br>Overlay histogram showing A431 cells stained with A03479-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT10A Antibody (A03479-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kap1-trim28-picoband-trade-antibody-m00409-1-boster.html2026-03-18T08:53:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-wb-testing-1.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) Western blot analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human PC-3 whole cell lysates;<br>
Lane 3: human HEK293 whole cell lysates;<br>
Lane 4: human A549 whole cell lysates;<br>
Lane 5: human Jurkat whole cell lysates;<br>
Lane 6: human THP-1 whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAP1/TRIM28 antigen affinity purified monoclonal antibody (Catalog # M00409-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAP1/TRIM28 at approximately 100KD. The expected band size for KAP1/TRIM28 is at 100KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-ihc-testing-2.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-ihc-testing-3.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-ihc-testing-4.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-ihc-testing-5.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-ihc-testing-6.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-if-testing-7.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IF analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human rectal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-if-testing-8.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IF analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-if-testing-9.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) IF analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-1). <br>
KAP1/TRIM28 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-KAP1/TRIM28 Antibody (M00409-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-1-kap1-trim28-primary-antibodies-fcm-testing-10.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 3H2) Flow Cytometry analysis of A549 cells using anti- KAP1/TRIM28 antibody (M00409-1). <br>Overlay histogram showing A549 cells stained with M00409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- KAP1/TRIM28 Antibody (M00409-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kap1-trim28-picoband-trade-antibody-m00409-2-boster.html2026-03-18T08:53:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-wb-testing-1.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) Western blot analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human PC-3 whole cell lysates;<br>
Lane 3: human HEK293 whole cell lysates;<br>
Lane 4: human A549 whole cell lysates;<br>
Lane 5: human Jurkat whole cell lysates;<br>
Lane 6: human THP-1 whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAP1/TRIM28 antigen affinity purified monoclonal antibody (Catalog # M00409-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAP1/TRIM28 at approximately 100KD. The expected band size for KAP1/TRIM28 is at 100KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-ihc-testing-2.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-ihc-testing-3.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-ihc-testing-4.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-if-testing-5.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IF analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-if-testing-6.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IF analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-fcm-testing-7.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) Flow Cytometry analysis of A549 cells using anti- KAP1/TRIM28 antibody (M00409-2). <br>Overlay histogram showing A549 cells stained with M00409-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- KAP1/TRIM28 Antibody (M00409-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-2-kap1-trim28-primary-antibodies-ihc-testing-8.jpgAnti-KAP1/TRIM28 Antibody Picoband® (monoclonal, 9E3) IHC analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). <br>
KAP1/TRIM28 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-KAP1/TRIM28 Antibody (M00409-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-ddx5-picoband-trade-antibody-m00670-1-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-wb-testing-1.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) Western blot analysis of DDX5 using anti-DDX5 antibody (M00670-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human SW620 whole cell lysates;<br>
Lane 3: human Caco-2 whole cell lysates;<br>
Lane 4: human K562 whole cell lysates;<br>
Lane 5: rat PC-12 whole cell lysates;<br>
Lane 6: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DDX5 antigen affinity purified monoclonal antibody (Catalog # M00670-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DDX5 at approximately 71KD. The expected band size for DDX5 is at 69KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-ihc-testing-2.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IHC analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-ihc-testing-3.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IHC analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-ihc-testing-4.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IHC analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-ihc-testing-5.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IHC analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-if-testing-6.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IF analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-if-testing-7.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IF analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-if-testing-8.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IF analysis of DDX5 using anti-DDX5 antibody (M00670-1). <br>
DDX5 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-DDX5 Antibody (M00670-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-fcm-testing-9_1.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) Flow Cytometry analysis of A431 cells using anti- DDX5 antibody (M00670-1). <br>Overlay histogram showing A431 cells stained with M00670-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX5 Antibody (M00670-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-1-ddx5-primary-antibodies-ihc-testing-10.jpgAnti-DDX5 Antibody Picoband® (monoclonal, 3F9) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M00670-1). <br>
Hexokinase 1/HK1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M00670-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-ribonuclease-inhibitor-rnh1-picoband-trade-antibody-m04147-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-wb-testing-1_1.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) Western blot analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human Jurkat whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human placenta tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ribonuclease Inhibitor/RNH1 antigen affinity purified monoclonal antibody (Catalog # M04147) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ribonuclease Inhibitor/RNH1 at approximately 45 kDa. The expected band size for Ribonuclease Inhibitor/RNH1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-ihc-testing-2.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) IHC analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>
Ribonuclease Inhibitor/RNH1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-ihc-testing-3.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) IHC analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>
Ribonuclease Inhibitor/RNH1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-ihc-testing-4.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) IHC analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>
Ribonuclease Inhibitor/RNH1 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-if-testing-5.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) IF analysis of Ribonuclease Inhibitor/RNH1 using anti-Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>
Ribonuclease Inhibitor/RNH1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Ribonuclease Inhibitor/RNH1 Antibody (M04147) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04147-rnh1-primary-antibodies-fcm-testing-6.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband® (monoclonal, 4F3) Flow Cytometry analysis of A549 cells using anti- Ribonuclease Inhibitor/RNH1 antibody (M04147). <br>Overlay histogram showing A549 cells stained with M04147 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ribonuclease Inhibitor/RNH1 Antibody (M04147, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rent1-hupf1-picoband-trade-antibody-m00900-boster.html2026-03-18T08:53:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-wb-testing-1_1.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) Western blot analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: rat brain tissue lysates;<br>
Lane 2: mouse brain tissue lysates;<br>
Lane 3: human RAW264.7 whole cell lysates;<br>
Lane 4: human HepG2 whole cell lysates;<br>
Lane 5: human Raji whole cell lysates;<br>
Lane 6: human PC-3 whole cell lysates;<br>
Lane 7: human Hela whole cell lysates;<br>
Lane 8: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RENT1/hUPF1 antigen affinity purified monoclonal antibody (Catalog # M00900) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RENT1/hUPF1 at approximately 130KD. The expected band size for RENT1/hUPF1 is at 130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-ihc-testing-2.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900). <br>
RENT1/hUPF1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RENT1/hUPF1 Antibody (M00900) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-ihc-testing-3.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900). <br>
RENT1/hUPF1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RENT1/hUPF1 Antibody (M00900) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-ihc-testing-4.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900). <br>
RENT1/hUPF1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RENT1/hUPF1 Antibody (M00900) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-ihc-testing-5.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900). <br>
RENT1/hUPF1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-RENT1/hUPF1 Antibody (M00900) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00900-rent1-hupf1-primary-antibodies-if-testing-6.jpgAnti-RENT1/hUPF1 Antibody Picoband® (monoclonal, 11E7) IF analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (M00900). <br>
RENT1/hUPF1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-RENT1/hUPF1 Antibody (M00900) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr2-picoband-trade-antibody-m00158-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-1-ccr2-primary-antibodies-wb-testing-1.jpgAnti-CCR2 Antibody Picoband® (monoclonal, 8C4) Western blot analysis of CCR2 using anti-CCR2 antibody (M00158-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human THP-1 whole cell lysates;<br>
Lane 2: human K562 whole cell lysates;<br>
Lane 3: human Jurkat whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CCR2 antigen affinity purified monoclonal antibody (Catalog # M00158-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42KD. The expected band size for CCR2 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-1-ccr2-primary-antibodies-ihc-testing-2.jpgAnti-CCR2 Antibody Picoband® (monoclonal, 8C4) IHC analysis of CCR2 using anti-CCR2 antibody (M00158-1). <br>
CCR2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CCR2 Antibody (M00158-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-1-ccr2-primary-antibodies-ihc-testing-3.jpgAnti-CCR2 Antibody Picoband® (monoclonal, 8C4) IHC analysis of CCR2 using anti-CCR2 antibody (M00158-1). <br>
CCR2 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CCR2 Antibody (M00158-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-1-ccr2-primary-antibodies-if-testing-4.jpgAnti-CCR2 Antibody Picoband® (monoclonal, 8C4) IF analysis of CCR2 using anti-CCR2 antibody (M00158-1). <br>
CCR2 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CCR2 Antibody (M00158-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00158-1-ccr2-primary-antibodies-fcm-testing-5.jpgAnti-CCR2 Antibody Picoband® (monoclonal, 8C4) Flow Cytometry analysis of THP-1 cells using anti- CCR2 antibody (M00158-1). <br>Overlay histogram showing THP-1 cells stained with M00158-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- CCR2 Antibody (M00158-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hexokinase-1-hk1-picoband-trade-antibody-m01504-1-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-wb-testing-1.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) Western blot analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates;<br>
Lane 3: human U-87MG whole cell lysates;<br>
Lane 4: human K562 whole cell lysates;<br>
Lane 5: human A549 whole cell lysates;<br>
Lane 6: rat PC-12 whole cell lysates;<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hexokinase 1/HK1 antigen affinity purified monoclonal antibody (Catalog # M01504-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Hexokinase 1/HK1 at approximately 120KD. The expected band size for Hexokinase 1/HK1 is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-ihc-testing-2.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-ihc-testing-3.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-ihc-testing-4.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-ihc-testing-5.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1). <br>
Hexokinase 1/HK1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-if-testing-6.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) IF analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-1). <br>
Hexokinase 1/HK1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Hexokinase 1/HK1 Antibody (M01504-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-1-hk1-primary-antibodies-fcm-testing-7.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) Flow Cytometry analysis of PC-3 cells using anti- Hexokinase 1/HK1 antibody (M01504-1). <br>Overlay histogram showing PC-3 cells stained with M01504-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Hexokinase 1/HK1 Antibody (M01504-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hexokinase-1-hk1-picoband-trade-antibody-m01504-2-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-wb-testing-1.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) Western blot analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates;<br>
Lane 3: human U-87MG whole cell lysates;<br>
Lane 4: human K562 whole cell lysates;<br>
Lane 5: human A549 whole cell lysates;<br>
Lane 6: rat PC-12 whole cell lysates;<br>
Lane 7: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hexokinase 1/HK1 antigen affinity purified monoclonal antibody (Catalog # M01504-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Hexokinase 1/HK1 at approximately 120KD. The expected band size for Hexokinase 1/HK1 is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-2.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-3.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-4.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-5.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-6.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-if-testing-7.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IF analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-if-testing-8.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IF analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-fcm-testing-9.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) Flow Cytometry analysis of PC-3 cells using anti- Hexokinase 1/HK1 antibody (M01504-2). <br>Overlay histogram showing PC-3 cells stained with M01504-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Hexokinase 1/HK1 Antibody (M01504-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01504-2-hk1-primary-antibodies-ihc-testing-10.jpgAnti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 4B7) IHC analysis of Hexokinase 1/HK1 using anti-Hexokinase 1/HK1 antibody (M01504-2). <br>
Hexokinase 1/HK1 was detected in a frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Hexokinase 1/HK1 Antibody (M01504-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-hspb8-hsp22-picoband-trade-antibody-m02492-2-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02492-2-hspb8-hsp22-primary-antibodies-wb-testing-1.jpgAnti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) Western blot analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (M02492-2).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Hela whole cell lysates;<br>
Lane 2: human T-47D whole cell lysates;<br>
Lane 3: rat RH35 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPB8/Hsp22 antigen affinity purified monoclonal antibody (Catalog # M02492-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPB8/Hsp22 at approximately 22KD. The expected band size for HSPB8/Hsp22 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02492-2-hspb8-hsp22-primary-antibodies-fcm-testing-2.jpgAnti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) Flow Cytometry analysis of CACO-2 cells using anti- HSPB8/Hsp22 antibody (M02492-2). <br>Overlay histogram showing CACO-2 cells stained with M02492-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HSPB8/Hsp22 Antibody (M02492-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02492-2-hspb8-hsp22-primary-antibodies-fcm-testing-3.jpgAnti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) Flow Cytometry analysis of U20S cells using anti- HSPB8/Hsp22 antibody (M02492-2). <br>Overlay histogram showing U20S cells stained with M02492-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HSPB8/Hsp22 Antibody (M02492-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02492-2-hspb8-primary-antibody-if-testing-4.jpgAnti-HSPB8/Hsp22 Antibody Picoband® (monoclonal, 7D8) IF analysis of HSPB8/Hsp22 using anti-HSPB8/Hsp22 antibody (M02492-2). <br>
HSPB8/Hsp22 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-HSPB8/Hsp22 Antibody (M02492-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bubr1-bub1b-picoband-trade-antibody-m01564-2-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-wb-testing-1.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates;<br>
Lane 3: human K562 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BubR1/BUB1B antigen affinity purified monoclonal antibody (Catalog # M01564-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 130KD. The expected band size for BubR1/BUB1B is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-ihc-testing-2.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-ihc-testing-3.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-ihc-testing-4.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-ihc-testing-5.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-fcm-testing-6.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) Flow Cytometry analysis of Hela cells using anti- BubR1/BUB1B antibody (M01564-2). <br>Overlay histogram showing Hela cells stained with M01564-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- BubR1/BUB1B Antibody (M01564-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-2-bubr1-bub1b-primary-antibodies-if-testing-7.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) IF analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-2). <br>
BubR1/BUB1B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-BubR1/BUB1B Antibody (M01564-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bubr1-bub1b-picoband-trade-antibody-m01564-3-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-3-bubr1-bub1b-primary-antibodies-wb-testing-1.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) Western blot analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-3).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates;<br>
Lane 3: human K562 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BubR1/BUB1B antigen affinity purified monoclonal antibody (Catalog # M01564-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BubR1/BUB1B at approximately 130KD. The expected band size for BubR1/BUB1B is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-3-bubr1-bub1b-primary-antibodies-ihc-testing-2.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-3). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-3-bubr1-bub1b-primary-antibodies-ihc-testing-3.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-3). <br>
BubR1/BUB1B was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-3-bubr1-bub1b-primary-antibodies-ihc-testing-4.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) IHC analysis of BubR1/BUB1B using anti-BubR1/BUB1B antibody (M01564-3). <br>
BubR1/BUB1B was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-BubR1/BUB1B Antibody (M01564-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01564-3-bubr1-bub1b-primary-antibodies-fcm-testing-5.jpgAnti-BubR1/BUB1B Antibody Picoband® (monoclonal, 8B3) Flow Cytometry analysis of Hela cells using anti- BubR1/BUB1B antibody (M01564-3). <br>Overlay histogram showing Hela cells stained with M01564-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- BubR1/BUB1B Antibody (M01564-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd31-pecam1-picoband-trade-antibody-m01513-4-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-4-cd31-pecam1-primary-antibodies-wb-testing-1.jpgAnti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
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Lane 1: human Jurkat whole cell lysates;<br>
Lane 2: human placenta tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD31/PECAM1 antigen affinity purified monoclonal antibody (Catalog # M01513-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 130KD. The expected band size for CD31/PECAM1 is at 130KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-4-cd31-pecam1-primary-antibodies-ihc-testing-2.jpgAnti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4). <br>
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-4-cd31-pecam1-primary-antibodies-ihc-testing-3.jpgAnti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4). <br>
CD31/PECAM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-4-cd31-pecam1-primary-antibodies-ihc-testing-4.jpgAnti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4). <br>
CD31/PECAM1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-4-cd31-pecam1-primary-antibodies-ihc-testing-5.jpgAnti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4) IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (M01513-4). <br>
CD31/PECAM1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD31/PECAM1 Antibody (M01513-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd146-mcam-picoband-trade-antibody-m01683-3-boster.html2026-03-24T05:26:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-wb-testing-1.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) Western blot analysis of CD146/MCAM using anti-CD146/MCAM antibody (M01683-3).
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Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human A375 whole cell lysates;<br>
Lane 2: human Hela whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD146/MCAM antigen affinity purified monoclonal antibody (Catalog # M01683-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD146/MCAM at approximately 120KD. The expected band size for CD146/MCAM is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-ihc-testing-2.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) IHC analysis of CD146/MCAM using anti-CD146/MCAM antibody (M01683-3). <br>
CD146/MCAM was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146/MCAM Antibody (M01683-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-ihc-testing-3.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) IHC analysis of CD146/MCAM using anti-CD146/MCAM antibody (M01683-3). <br>
CD146/MCAM was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146/MCAM Antibody (M01683-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-ihc-testing-4.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) IHC analysis of CD146/MCAM using anti-CD146/MCAM antibody (M01683-3). <br>
CD146/MCAM was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146/MCAM Antibody (M01683-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-ihc-testing-5.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) IHC analysis of CD146/MCAM using anti-CD146/MCAM antibody (M01683-3). <br>
CD146/MCAM was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146/MCAM Antibody (M01683-3) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-3-cd146-mcam-primary-antibodies-fcm-testing-6.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) Flow Cytometry analysis of SiHa cells using anti- CD146/MCAM antibody (M01683-3). <br>Overlay histogram showing SiHa cells stained with M01683-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- CD146/MCAM Antibody (M01683-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd146-mcam-picoband-trade-antibody-m01683-4-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-4-mcam-primary-antibodies-wb-testing-1.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 2H12) Western blot analysis of CD146 using anti-CD146 antibody (M01683-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human A375 whole cell lysates, <br>
Lane 3: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD146 antigen affinity purified monoclonal antibody (Catalog # M01683-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120KD. The expected band size for CD146 is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-4-mcam-primary-antibodies-ihc-testing-2.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 2H12) IHC analysis of CD146 using anti-CD146 antibody (M01683-4). <br>
CD146 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146 Antibody (M01683-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-4-mcam-primary-antibodies-ihc-testing-3_1.jpgAnti-CD146/MCAM Antibody Picoband® (monoclonal, 2H12) IHC analysis of CD146 using anti-CD146 antibody (M01683-4). <br>
CD146 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD146 Antibody (M01683-4) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-1-catenin-ctnna1-picoband-trade-antibody-m01617-1-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01617-1-ctnna1-primary-antibodies-wb-testing-1.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2) Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (M01617-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human HEK293 whole cell lysates;<br>
Lane 3: human U20S whole cell lysates;<br>
Lane 4: human U-87MG whole cell lysates;<br>
Lane 5: rat PC-12 whole cell lysates;<br>
Lane 6: mouse NIH/3T3 whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CTNNA1 antigen affinity purified monoclonal antibody (Catalog # M01617-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 100KD. The expected band size for CTNNA1 is at 100KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01617-1-ctnna1-primary-antibodies-ihc-testing-2.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2) IHC analysis of CTNNA1 using anti-CTNNA1 antibody (M01617-1). <br>
CTNNA1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CTNNA1 Antibody (M01617-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01617-1-ctnna1-primary-antibodies-fcm-testing-3.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2) Flow Cytometry analysis of Jurkat cells using anti-CTNNA1 antibody (M01617-1).Overlay histogram showing Jurkat cells stained with M01617-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-CTNNA1 Antibody (M01617-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thioredoxin-2-txn2-picoband-trade-antibody-m04586-1-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-wb-testing-1_1.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) Western blot analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human Raji whole cell lysates;<br>
Lane 3: human K562 whole cell lysates;<br>
Lane 4: human A549 whole cell lysates;<br>
Lane 5: human Caco-2 whole cell lysates;<br>
Lane 6: human THP-1 whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse RAW264.7 whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Thioredoxin 2/TXN2 antigen affinity purified monoclonal antibody (Catalog # M04586-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 14KD. The expected band size for CTNNA1 is at 14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-if-testing-2.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) IF analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-1). <br>
Thioredoxin 2/TXN2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-fcm-testing-3.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) Flow Cytometry analysis of HL-60 cells using anti-Thioredoxin 2/TXN2 antibody (M04586-1).<br>Overlay histogram showing HL-60 cells stained with M04586-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-ihc-testing-4.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-1). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-ihc-testing-5.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-1). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-1-txn2-primary-antibodies-ihc-testing-6.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 7B5) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-1). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thioredoxin-2-txn2-picoband-trade-antibody-m04586-2-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-wb-testing-1_1.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) Western blot analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human Raji whole cell lysates;<br>
Lane 3: human K562 whole cell lysates;<br>
Lane 4: human A549 whole cell lysates;<br>
Lane 5: human Caco-2 whole cell lysates;<br>
Lane 6: human THP-1 whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse RAW264.7 whole cell lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Thioredoxin 2/TXN2 antigen affinity purified monoclonal antibody (Catalog # M04586-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 14KD. The expected band size for CTNNA1 is at 14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-if-testing-2.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IF analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-if-testing-3.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IF analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-fcm-testing-4.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) Flow Cytometry analysis of HL-60 cells using anti-Thioredoxin 2/TXN2 antibody (M04586-2).<br>Overlay histogram showing HL-60 cells stained with M04586-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-if-testing-5.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IF analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-ihc-testing-6.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-ihc-testing-7.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04586-2-txn2-primary-antibodies-ihc-testing-8.jpgAnti-Thioredoxin 2/TXN2 Antibody Picoband® (monoclonal, 4H3) IHC analysis of Thioredoxin 2/TXN2 using anti-Thioredoxin 2/TXN2 antibody (M04586-2). <br>
Thioredoxin 2/TXN2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Thioredoxin 2/TXN2 Antibody (M04586-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-p-cadherin-cdh3-picoband-trade-antibody-m03353-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03353-p_cadherin-cdh3-primary-antibodies-wb-testing-1.jpgAnti-P-Cadherin-3 CDH3 Antibody Picoband® (monoclonal, 3C9) Western blot analysis of P cadherin/CDH3 using anti-P cadherin/CDH3 antibody (M03353).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human A431 whole cell lysates;<br>
Lane 2: human Caco-2 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-P cadherin/CDH3 antigen affinity purified monoclonal antibody (Catalog # M03353) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for P cadherin/CDH3 at approximately 120KD. The expected band size for P cadherin/CDH3 is at 91KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03353-p-cadherin-primary-antibodies-if-testing-2.jpgAnti-P-Cadherin-3 CDH3 Antibody Picoband® (monoclonal, 3C9) IF analysis of P cadherin using anti-P cadherin antibody (M03353). <br>
P cadherin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-P cadherin Antibody (M03353) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-zap70-picoband-trade-antibody-m00754-5-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-5-zap70-primary-antibodies-wb-testing-1.jpgAnti-ZAP70 Antibody Picoband® (monoclonal, 9D5) Western blot analysis of ZAP70 using anti-ZAP70 antibody (M00754-5).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Jurkat whole cell lysates;<br>
Lane 2: human CCRF-CEM whole cell lysates;<br>
Lane 3: rat thymus tissue lysates;<br>
Lane 4: mouse spleen tissue lysates;<br>
Lane 5: mouse thymus tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ZAP70 antigen affinity purified monoclonal antibody (Catalog # M00754-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 72KD. The expected band size for ZAP70 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-5-zap70-primary-antibodies-fcm-testing-2.jpgAnti-ZAP70 Antibody Picoband® (monoclonal, 9D5) Flow Cytometry analysis of 293T cells using anti- ZAP70 antibody (M00754-5). <br>Overlay histogram showing 293T cells stained with M00754-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- ZAP70 Antibody (M00754-5, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00754-5-zap70-primary-antibodies-fcm-testing-3.jpgAnti-ZAP70 Antibody Picoband® (monoclonal, 9D5) Flow Cytometry analysis of Jurkat cells using anti- ZAP70 antibody (M00754-5). <br>Overlay histogram showing Jurkat cells stained with M00754-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- ZAP70 Antibody (M00754-5, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-akr1d1-picoband-trade-antibody-m05278-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05278-akr1d1-primary-antibodies-wb-testing-1.jpgAnti-AKR1D1 Antibody Picoband® (monoclonal, 6I4) Western blot analysis of AKR1D1 using anti-AKR1D1 antibody (M05278). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates;<br>
Lane 2: human HL-60 whole cell lysates;<br>
Lane 3: human THP-1 whole cell lysates;<br>
Lane 4: rat liver tissue lysates;<br>
Lane 5: rat RH35 whole cell lysates;<br>
Lane 6: mouse liver tissue lysates;<br>
Lane 7: mouse HEPA1-6 whole cell lysates;<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKR1D1 antigen affinity purified monoclonal antibody (Catalog # M05278) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AKR1D1 at approximately 37KD. The expected band size for AKR1D1 is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05278-akr1d1-primary-antibodies-ihc-testing-2.jpgAnti-AKR1D1 Antibody Picoband® (monoclonal, 6I4) IHC analysis of AKR1D1 using anti-AKR1D1 antibody (M05278). <br>
AKR1D1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-AKR1D1 Antibody (M05278) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05278-akr1d1-primary-antibodies-ihc-testing-3.jpgAnti-AKR1D1 Antibody Picoband® (monoclonal, 6I4) IHC analysis of AKR1D1 using anti-AKR1D1 antibody (M05278). <br>
AKR1D1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-AKR1D1 Antibody (M05278) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05278-akr1d1-primary-antibodies-fcm-testing-4.jpgAnti-AKR1D1 Antibody Picoband® (monoclonal, 6I4) Flow Cytometry analysis of HepG2 cells using anti-AKR1D1 antibody (M05278).<br>Overlay histogram showing HepG2 cells stained with M04586-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AKR1D1 Antibody (M05278,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-retinoid-x-receptor-alpha-rxra-picoband-trade-antibody-m01299-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01299-rxra-primary-antibodies-wb-testing-1.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) Western blot analysis of Retinoid X Receptor alpha/RXRA using anti-Retinoid X Receptor alpha/RXRA antibody (M01299).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human A549 whole cell lysates;<br>
Lane 3: human HepG2 whole cell lysates;<br>
Lane 4: human Caco-2 whole cell lysates;<br>
Lane 5: human HL-60 whole cell lysates;<br>
Lane 6: rat liver tissue lysates;<br>
Lane 7: mouse liver tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Retinoid X Receptor alpha/RXRA antigen affinity purified monoclonal antibody (Catalog # M01299) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Retinoid X Receptor alpha/RXRA at approximately 55KD. The expected band size for Retinoid X Receptor alpha/RXRA is at 51KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01299-rxra-primary-antibodies-fcm-testing-2.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) Flow Cytometry analysis of A549 cells using anti- Retinoid X Receptor alpha/RXRA antibody (M01299). <br>Overlay histogram showing A549 cells stained with M01299 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01299-rxra-primary-antibodies-fcm-testing-3.jpgAnti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) Flow Cytometry analysis of CACO-2 cells using anti- Retinoid X Receptor alpha/RXRA antibody (M01299). <br>Overlay histogram showing CACO-2 cells stained with M01299 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smarca2-brm-picoband-trade-antibody-m01888-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01888-smarca2-primary-antibodies-wb-testing-1.jpgAnti-SMARCA2/BRM Antibody Picoband® (monoclonal, 3G3) Western blot analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (M01888). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human THP-1 whole cell lysates, <br>
Lane 3: human U87 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMARCA2/BRM antigen affinity purified monoclonal antibody (Catalog # M01888) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SMARCA2/BRM at approximately 210KD. The expected band size for SMARCA2/BRM is at 181KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01888-smarca2-primary-antibodies-if-testing-2.jpgAnti-SMARCA2/BRM Antibody Picoband® (monoclonal, 3G3) IF analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (M01888). <br>
SMARCA2/BRM was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-SMARCA2/BRM Antibody (M01888) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01888-smarca2-primary-antibodies-fcm-testing-3.pngAnti-SMARCA2/BRM Antibody Picoband® (monoclonal, 3G3) Flow Cytometry analysis of A431 cells using anti- SMARCA2/BRM antibody (M01888). <br>Overlay histogram showing A431 cells stained with M01888 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SMARCA2/BRM Antibody (M01888, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smarca2-brm-picoband-trade-antibody-m01888-1-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01888-1-smarca2-brm-primary-antibodies-wb-testing-1.jpgAnti-SMARCA2/BRM Antibody Picoband® (monoclonal, 4E8) Western blot analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (M01888-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human THP-1 whole cell lysates;<br>
Lane 3: human U-87MG whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMARCA2/BRM antigen affinity purified monoclonal antibody (Catalog # M01888-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SMARCA2/BRM at approximately 210KD. The expected band size for SMARCA2/BRM is at 181KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01888-1-smarca2-brm-primary-antibodies-if-testing-2.jpgAnti-SMARCA2/BRM Antibody Picoband® (monoclonal, 4E8) IF analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (M01888-1). <br>
SMARCA2/BRM was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-SMARCA2/BRM Antibody (M01888-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-14-3-3-zeta-delta-ywhaz-picoband-trade-antibody-m01141-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01141-ywhaz-primary-antibodies-wb-testing-1.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human A549 whole cell lysates;<br>
Lane 3: monkey COS-7 whole cell lysates;<br>
Lane 4: human Raji whole cell lysates;<br>
Lane 5:huamn Caco-2 whole cell lysates;<br>
Lane 6: huamn Jurkat whole cell lysates;<br>
Lane 7: mouse brain tissue lysates;<br>
Lane 8: rat brain tissue lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01141-ywhaz-primary-antibodies-fcm-testing-2.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) Flow Cytometry analysis of PC-3 cells using anti-14-3-3 zeta/delta antibody (M01141).<br>Overlay histogram showing PC-3 cells stained with M01141 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 zeta/delta Antibody (M01141,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01141-ywhaz-primary-antibodies-fcm-testing-3.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6G5) Flow Cytometry analysis of SiHa cells using anti-14-3-3 zeta/delta antibody (M01141).<br>Overlay histogram showing SiHa cells stained with M01141 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 zeta/delta Antibody (M01141,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-14-3-3-zeta-delta-ywhaz-picoband-trade-antibody-m01141-1-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01141-1-ywhaz-primary-antibodies-wb-testing-1.jpgAnti-14-3-3 zeta/delta/YWHAZ Antibody Picoband® (monoclonal, 6H7) Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (M01141-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human A549 whole cell lysates;<br>
Lane 3: monkey COS-7 whole cell lysates;<br>
Lane 4: human Raji whole cell lysates;<br>
Lane 5: huamn Caco-2 whole cell lysates;<br>
Lane 6: huamn Jurkat whole cell lysates;<br>
Lane 7: mouse brain tissue lysates;<br>
Lane 8: rat brain tissue lysates<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-14-3-3 zeta/delta antigen affinity purified monoclonal antibody (Catalog # M01141-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.
https://www.bosterbio.com/products/primary-antibodies/anti-methylmalonyl-coenzyme-a-mutase-picoband-trade-antibody-m01065-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01065-methylmalonyl-coenzyme-a-primary-antibodies-wb-testing-1.jpgAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) Western blot analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates;<br>
Lane 2: human K562 whole cell lysates;<br>
Lane 3: human HEK293 whole cell lysates;<br>
Lane 4: human PC-3 whole cell lysates;<br>
Lane 5: human Caco-2 whole cell lysates;<br>
Lane 6: human Raji whole cell lysates;<br>
Lane 7: rat PC-12 whole cell lysates;<br>
Lane 8: mouse NIH/3T3 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Methylmalonyl Coenzyme A antigen affinity purified monoclonal antibody (Catalog # M01065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Methylmalonyl Coenzyme A at approximately 83KD. The expected band size for Methylmalonyl Coenzyme A is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01065-methylmalonyl-coenzyme-a-primary-antibodies-ihc-testing-2.jpgAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065). <br>
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01065-methylmalonyl-coenzyme-a-primary-antibodies-ihc-testing-3.jpgAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065). <br>
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01065-methylmalonyl-coenzyme-a-primary-antibodies-ihc-testing-4.jpgAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065). <br>
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01065-methylmalonyl-coenzyme-a-primary-antibodies-ihc-testing-5.jpgAnti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065). <br>
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-bid-picoband-trade-antibody-m00730-1-boster.html2026-03-24T05:26:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00730-1-bid-primary-antibodies-wb-testing-1.jpgAnti-Bid Antibody Picoband® (monoclonal, 10F4) Western blot analysis of Bid using anti-Bid antibody (M00730-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Jurkat whole cell lysates;<br>
Lane 2: human A549 whole cell lysates;<br>
Lane 3: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Bid antigen affinity purified monoclonal antibody (Catalog # M00730-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Bid at approximately 22KD. The expected band size for Bid is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00730-1-bid-primary-antibodies-ihc-testing-2.jpgAnti-Bid Antibody Picoband® (monoclonal, 10F4) IHC analysis of Bid using anti-Bid antibody (M00730-1). <br>
Bid was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Bid Antibody (M00730-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00730-1-bid-primary-antibodies-ihc-testing-3.jpgAnti-Bid Antibody Picoband® (monoclonal, 10F4) IHC analysis of Bid using anti-Bid antibody (M00730-1). <br>
Bid was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Bid Antibody (M00730-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00730-1-bid-primary-antibodies-fcm-testing-4_1.jpgAnti-Bid Antibody Picoband® (monoclonal, 10F4) Flow Cytometry analysis of A549 cells using anti- Bid antibody (M00730-1). <br>Overlay histogram showing A549 cells stained with M00730-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Bid Antibody (M00730-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00730-1-bid-primary-antibodies-if-testing-5.jpgAnti-Bid Antibody Picoband® (monoclonal, 10F4) IF analysis of Bid using anti-Bid antibody (M00730-1). <br>
Bid was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti-Bid Antibody (M00730-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/catalog/product/view/id/557562026-03-24T05:26:14+00:00daily0.9
https://www.bosterbio.com/catalog/product/view/id/557572026-03-24T05:26:14+00:00daily0.9
https://www.bosterbio.com/catalog/product/view/id/557582026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz33989-1-clipb4-primary-antibodies-wb-testing-1.jpgAnti-African malaria mosquito CLIPB4 AntibodyWestern blot analysis of CLIPB4 using anti-CLIPB4 antibody (DZ33989-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: mosquito hemolymph β-galactosidase KD lysates,<br>
Lane 2: mosquito hemolymph CLIPB4 KD lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-CLIPB4 antigen affinity purified monoclonal antibody (DZ33989-1) at 1:3000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody at a dilution of 1:15000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for CLIPB4 at approximately 40 kDa. The expected band size for CLIPB4 is at 39 kDa.
https://www.bosterbio.com/catalog/product/view/id/557592026-03-24T05:26:14+00:00daily0.9
https://www.bosterbio.com/catalog/product/view/id/557602026-03-24T05:26:14+00:00daily0.9
https://www.bosterbio.com/products/primary-antibodies/anti-atg4a-picoband-trade-antibody-a06539-2-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-2-atg4a-primary-antibodies-wb-testing-1.jpgAnti-ATG4A Antibody Picoband® Western blot analysis of ATG4A using anti-ATG4A antibody (A06539-2).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human PC-3 whole cell lysates,<br>
Lane 4: human A431 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4A antigen affinity purified polyclonal antibody (Catalog # A06539-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4A at approximately 50KD. The expected band size for ATG4A is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-2-atg4a-primary-antibodies-ihc-testing-2.jpgAnti-ATG4A Antibody Picoband® IHC analysis of ATG4A using anti-ATG4A antibody (A06539-2). <br>
ATG4A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATG4A Antibody (A06539-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-2-atg4a-primary-antibodies-ihc-testing-3.jpgAnti-ATG4A Antibody Picoband® IHC analysis of ATG4A using anti-ATG4A antibody (A06539-2). <br>
ATG4A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATG4A Antibody (A06539-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-2-atg4a-primary-antibodies-fcm-testing-4.jpgAnti-ATG4A Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-ATG4A antibody (A06539-2).<br>Overlay histogram showing PC-3 cells stained with A06539-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4A Antibody (A06539-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-atg7-picoband-trade-antibody-a00346-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-wb-testing-1_1.jpgAnti-ATG7 Antibody Picoband®Western blot analysis of ATG7 using anti-ATG7 antibody (A00346). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates. <br>
Lane 2: human PC-3 whole cell lysates. <br>
Lane 3: rat spleen tissue lysates. <br>
Lane 4: mouse spleen tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG7 antigen affinity purified polyclonal antibody (A00346) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATG7 at approximately 78 kDa. The expected band size for ATG7 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-ihc-testing-1.jpgAnti-ATG7 Antibody Picoband®IHC analysis of ATG7 using anti-ATG7 antibody (A00346). <br>ATG7 was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG7 Antibody (A00346) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-ihc-testing-2.jpgAnti-ATG7 Antibody Picoband®IHC analysis of ATG7 using anti-ATG7 antibody (A00346). <br>ATG7 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG7 Antibody (A00346) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-ihc-testing-3.jpgAnti-ATG7 Antibody Picoband®IHC analysis of ATG7 using anti-ATG7 antibody (A00346). <br>ATG7 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG7 Antibody (A00346) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-if-testing-1.jpgAnti-ATG7 Antibody Picoband®IF analysis of ATG7 using anti-ATG7 antibody (A00346). <br>ATG7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG7 Antibody (A00346) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00346-atg7-primary-antibodies-fcm-testing-1.jpgAnti-ATG7 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-ATG7 antibody (A00346). <br>Overlay histogram showing HepG2 cells stained with A00346 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG7 Antibody (A00346, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-p57-kip2-cdkn1c-picoband-trade-antibody-a01244-1-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01244-1-cdkn1c-primary-antibodies-wb-testing-1.jpgAnti-p57 Kip2/CDKN1C Antibody Picoband® Western blot analysis of p57 Kip2/CDKN1C using anti-p57 Kip2/CDKN1C antibody (A01244-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human SW620 whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p57 Kip2/CDKN1C antigen affinity purified polyclonal antibody (Catalog # A01244-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p57 Kip2/CDKN1C at approximately 57KD. The expected band size for p57 Kip2/CDKN1C is at 57KD.
https://www.bosterbio.com/products/primary-antibodies/anti-eno1-picoband-trade-antibody-a01250-1-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-wb-testing-1.jpgAnti-ENO1 Antibody Picoband® Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human SH-SY5Y whole cell lysates,<br>
Lane 4: human U-87MG whole cell lysates,<br>
Lane 5: human HEK293 whole cell lysates,<br>
Lane 6: human Caco-2 whole cell lysates,<br>
Lane 7: monkey kidney tissue lysates,<br>
Lane 8: monkey liver tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47KD. The expected band size for ENO1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-wb-testing-2.jpgAnti-ENO1 Antibody Picoband® Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat heart tissue lysates, <br>
Lane 3: rat kidney tissue lysates,<br>
Lane 4: rat liver tissue lysates,<br>
Lane 5: mouse brain tissue lysates,<br>
Lane 6: mouse kidney tissue lysates,<br>
Lane 7: mouse liver tissue lysates,<br>
Lane 8: mouse RAW264.7 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47KD. The expected band size for ENO1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-ihc-testing-3.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1). <br>
ENO1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ENO1 Antibody (A01250-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-ihc-testing-4.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1). <br>
ENO1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ENO1 Antibody (A01250-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-ihc-testing-5.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-1). <br>
ENO1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ENO1 Antibody (A01250-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-ip-testing-1.jpgAnti-ENO1 Antibody Picoband®Immunoprecipitating (IP) ENO1 in A549 whole cell lysate.<br>
Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1); <br>
Lane 1: A549 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-ENO1 antibody in A549 whole cell lysate;<br>
Lane 3: anti-ENO1 antibody (2μg) + A549 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (A01250-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ENO1 at approximately 47 kDa. The expected band size for ENO1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-if-testing-6.jpgAnti-ENO1 Antibody Picoband® IF analysis of ENO1 using anti-ENO1 antibody (A01250-1). <br>
ENO1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ENO1 Antibody (A01250-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-1-eno1-primary-antibodies-fcm-testing-7.jpgAnti-ENO1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ENO1 antibody (A01250-1).<br>Overlay histogram showing HL-60 cells stained with A01250-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENO1 Antibody (A01250-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-gap43-picoband-trade-antibody-a01868-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01868-gap43-primary-antibodies-wb-testing-1.jpgAnti-GAP43 Antibody Picoband® Western blot analysis of GAP43 using anti-GAP43 antibody (A01868). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates, <br>
Lane 3: human U87 whole cell lysates, <br>
Lane 4: mouse Neuro-2a whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # A01868) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 38-43KD. The expected band size for GAP43 is at 38-43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01868-gap43-primary-antibodies-ihc-testing-2.jpgAnti-GAP43 Antibody Picoband® IHC analysis of GAP43 using anti-GAP43 antibody (A01868). <br>
GAP43 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GAP43 Antibody (A01868) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01868-gap43-primary-antibodies-fcm-testing-3.pngAnti-GAP43 Antibody Picoband® Flow Cytometry analysis of HEPG2 cells using anti-GAP43 antibody (A01868). <br>Overlay histogram showing HEPG2 cells stained with A01868 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAP43 Antibody (A01868, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01868-gap43-primary-antibodies-if-testing-4.jpgAnti-GAP43 Antibody Picoband® IF analysis of GAP43 using anti-GAP43 antibody (A01868). <br>
GAP43 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-GAP43 Antibody (A01868) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-gsk3-alpha-gsk3a-picoband-trade-antibody-a03152-4-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03152-4-gsk3a-primary-antibodies-wb-testing-1.jpgAnti-GSK3 alpha/GSK3A Antibody Picoband® Western blot analysis of GSK3 alpha/GSK3A using anti-GSK3 alpha/GSK3A antibody (A03152-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HELA whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha/GSK3A antigen affinity purified polyclonal antibody (Catalog # A03152-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha/GSK3A at approximately 51KD. The expected band size for GSK3 alpha/GSK3A is at 51KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ngf-picoband-trade-antibody-a00341-1-boster.html2026-03-24T05:26:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00341-1-ngf-primary-antibodies-wb-testing-1.jpgAnti-NGF Antibody Picoband® Western blot analysis of NGF using anti-NGF antibody (A00341-1).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human U-87MG whole cell lysates, <br>
Lane 2: human 22RV1 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27KD. The expected band size for NGF is at 27KD.
https://www.bosterbio.com/products/primary-antibodies/anti-sdha-picoband-trade-antibody-a01753-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01753-sdha-primary-antibodies-if-testing-1.jpgAnti-SDHA Antibody Picoband® IF analysis of SDHA using anti-SDHA antibody (A01753). <br>
SDHA was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SDHA Antibody (A01753) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01753-sdha-primary-antibodies-wb-testing-2.jpgAnti-SDHA Antibody Picoband® Western blot analysis of SDHA using anti-SDHA antibody (A01753).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates,<br>
Lane 4: human Caco-2 whole cell lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: rat kidney tissue lysates,<br>
Lane 7: rat spleen tissue lysates,<br>
Lane 8: rat liver tissue lysates,<br>
Lane 9: mouse brain tissue lysates,<br>
Lane 10: mouse kidney tissue lysates,<br>
Lane 11: mouse liver tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (Catalog # A01753) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73KD. The expected band size for SDHA is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01753-sdha-primary-antibodies-fcm-testing-3.jpgAnti-SDHA Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SDHA antibody (A01753).<br>Overlay histogram showing Hela cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01753-sdha-primary-antibodies-fcm-testing-4.jpgAnti-SDHA Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-SDHA antibody (A01753).<br>Overlay histogram showing RAW264.7 cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01753-sdha-primary-antibodies-fcm-testing-5.jpgAnti-SDHA Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-SDHA antibody (A01753).<br>Overlay histogram showing C6 cells stained with A01753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (A01753,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sgk1-picoband-trade-antibody-a00673-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00673-sgk1-primary-antibodies-if-testing-1.jpgAnti-SGK1 Antibody Picoband® IF analysis of SGK1 using anti-SGK1 antibody (A00673). <br>
SGK1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SGK1 Antibody (A00673) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00673-sgk1-primary-antibodies-fcm-testing-2.jpgAnti-SGK1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SGK1 antibody (A00673). <br>Overlay histogram showing SiHa cells stained with A00673 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGK1 Antibody (A00673, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00673-sgk1-primary-antibodies-wb-testing-3.jpgAnti-SGK1 Antibody Picoband® Western blot analysis of SGK1 using anti-SGK1 antibody (A00673).
<br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: monkey kidney tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # A00673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 55KD. The expected band size for SGK1 is at 49KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00673-sgk1-primary-antibodies-ihc-testing-4.jpgAnti-SGK1 Antibody Picoband® IHC analysis of SGK1 using anti-SGK1 antibody (A00673). <br>
SGK1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SGK1 Antibody (A00673) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00673-sgk1-primary-antibodies-ihc-testing-5.jpgAnti-SGK1 Antibody Picoband® IHC analysis of SGK1 using anti-SGK1 antibody (A00673). <br>
SGK1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SGK1 Antibody (A00673) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-slc10a1-ntcp1-picoband-trade-antibody-a06872-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06872-slc10a1-primary-antibodies-wb-testing-1.jpgAnti-SLC10A1/NTCP1 Antibody Picoband® Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1antibody (A06872). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 50KD. The expected band size for SLC10A1/NTCP1 is at 50KD.
https://www.bosterbio.com/products/primary-antibodies/anti-smad5-picoband-trade-antibody-a01423-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01423-smad5-primary-antibodies-wb-testing-1_1.jpgAnti-SMAD5 Antibody Picoband® Western blot analysis of SMAD5 using anti-SMAD5 antibody (A01423). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates,<br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4:monkey COS-7 whole cell lysates,<br>
Lane 5: human Caco-2 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 antigen affinity purified polyclonal antibody (Catalog # A01423) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5 at approximately 52-60 kDa. The expected band size for SMAD5 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01423-smad5-primary-antibodies-wb-testing-2.jpgAnti-SMAD5 Antibody Picoband® Western blot analysis of SMAD5 using anti-SMAD5 antibody (A01423). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates,<br>
Lane 2: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 antigen affinity purified polyclonal antibody (Catalog # A01423) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5 at approximately 52-60 kDa. The expected band size for SMAD5 is at 52 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-beta-3-adrenergic-receptor-adrb3-picoband-trade-antibody-a02104-3-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02104-3-adrb3-primary-antibodies-if-testing-1.jpgAnti-beta 3 Adrenergic Receptor/ADRB3 Antibody Picoband® IF analysis of ADRB3 using anti-ADRB3 antibody (A02104-3). <br>
ADRB3 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADRB3 Antibody (A02104-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02104-3-adrb3-primary-antibodies-wb-testing-2.jpgAnti-beta 3 Adrenergic Receptor/ADRB3 Antibody Picoband® Western blot analysis of ADRB3 using anti-ADRB3 antibody (A02104-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse liver tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRB3 antigen affinity purified polyclonal antibody (Catalog # A02104-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRB3 at approximately 44KD. The expected band size for ADRB3 is at 44KD.
https://www.bosterbio.com/products/primary-antibodies/anti-aryl-hydrocarbon-receptor-ahr-picoband-trade-antibody-a00225-4-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g005.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR directly regulates Mrp1 transcription in mHSCs. (A–C) The expression of Ahr, Mrp1 or Cyp1a1 was detected by QPCR. (D) There are two potential AHR exogenous response elements on the Mrp1 promoter sequence. (E) Promoter sequences containing XREL1 and XREL2 were cloned onto PGL3 plasmids, and mutant plasmids were constructed. (F) Double luciferase assay to detect luciferase activity. (G) EMSA detects AHR binding to specific elements XREL1 and XREL2. (H) CHIP detects AHR binding to specific elements XREL1 and XREL2. Data are expressed as means ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001; Student's t ‐test or one‐way ANOVA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g004.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR reduces the antioxidant capacity of mHSCs. (A) Analysis of GSH content changes. (B) Analysis of GSH content changes. (C) Analysis of GSH content changes. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; Student's t ‐test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g001.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR increases the sensitivity of HSC to ferroptosis. (A) Morphological changes in mitochondria were detected by electron microscopy. Quantification of mitochondria sizes. Scale bar = 1 μm. (B) TMRE fluorescent probe was used to analyze the change of mitochondrial membrane potential in mHSCs. The change of mitochondrial membrane potential in mHSCs was quantitatively analyzed. Scale bar = 50 μm (C) TMRE fluorescent probe was used to analyze the change of mitochondrial membrane potential in primary mouse hepatocytes. The change of mitochondrial membrane potential in primary mouse hepatocytes was quantitatively analyzed. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; Student's t ‐test. Scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g007.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR promotes mHSC ferroptosis. (A) Ferroorange fluorescent probe was used to analyze the change of Fe 2+ content in mHSCs. The change of Fe 2+ content in HSCs was quantitatively analyzed. (B) H2DCFDA fluorescent probe was used to analyze the change of ROS content in mHSCs. The change of ROS content in HSCs was quantitatively analyzed. (C) BODIPY 581/591 C11 fluorescent probe was used to analyze the change of MDA content in mHSCs. The change of MDA content in mHSCs was quantitatively analyzed. (D) QPCR analysis of iron death related gene expression changes. (E) Analysis of intracellular GPXs activity. (F) Analysis of cell mortality. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; Student's t ‐test. Scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g006.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR improved the antioxidant capacity of primary mouse hepatocytes. (A) Ferroorange fluorescent probe was used to analyze the change of Fe 2+ content in primary mouse hepatocytes. The change of Fe 2+ content in primary mouse hepatocytes was quantitatively analyzed. (B) H2DCFDA fluorescent probe was used to analyze the change of ROS content in primary mouse hepatocytes. The change of ROS content in primary mouse hepatocytes was quantitatively analyzed. (C) BODIPY 581/591 C11 fluorescent probe was used to analyze the change of MDA content in primary mouse hepatocytes. The change of MDA content in primary mouse hepatocytes was quantitatively analyzed. (D) QPCR analysis of iron death related gene expression changes in primary mouse hepatocytes. (E) Analysis of intracellular GPXs activity. (F) Analysis of cell mortality. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; Student's t ‐test. Scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g003.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR alleviates liver fibrosis. (A) The WT mice received intraperitoneal paraffin oil injection (control group) or CCl4 injection twice per week for 4 weeks and these mice were orally gavaged with either Vehicle or YH439 once a day for a week. (B) Expressions of Cyp1a1 were detected by QPCR ( n = 5). (C) The changes in ALT and AST levels were detected ( n = 5). (D) Expressions of αSma, Col1a1 , and Col1a2 were detected by QPCR ( n = 5). (E) αSMA Immunofluorescence staining and H&E staining. The scales are 50 or 100 μm, respectively. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; one‐way ANOVA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-jcmm-28-e70278-g002.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®AHR alleviates liver fibrosis and is abolished in HSC‐specific Mrp1 deficient (Mrp1 −/− ) mice. (A)The Mrp1 fl/fl mice or Mrp1 −/− mice received intraperitoneal paraffin oil injection (control group) or CCl4 injection twice per week for 4 weeks and these mice were orally gavaged with either Vehicle or YH439 once a day for a week. (B) Expressions of Cyp1a1 were detected by QPCR ( n = 5). (C) Expressions of αSma, Col1a1 , and Col1a2 were detected by QPCR ( n = 5). (D) αSMA Immunofluorescence staining and H&E staining. The scales are 50 μm or 100 μm, respectively. Data are expressed as means ± SD; * p < 0.05 and ** p < 0.01; one‐way ANOVA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11628353/'>39654034</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-fnut-09-896815-g0006.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®ICA accelerates the proliferation of intestinal epithelial cells in vitro . IPEC-J2 cells were treated with different doses of ICA for 24 h. The cell viability was determined by CCK8 assay (A) . The cell proliferation was determined by EdU incorporation assay (B,C) . Cells were stained for EdU (red), and the nuclei were stained with DAPI (blue). The expression of AHR, PCNA, and Cyclin D1 were measured by western blot (D) . Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the Control group. ICA100, the basal diet supplemented with 100 mg/kg indole-3-carboxaldehyde. AHR, aryl hydrocarbon receptor; PCNA, proliferating cell nuclear antigen.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9149414/&orig_host=www.ncbi.nlm.nih.gov'>35651506</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-fcell-13-1622998-g003.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®Kyn impairs decidualization of mouse stromal cells and activates AhR. (A) Prl8a2 and Prl3c1 mRNA levels after stromal cells were treated with Kyn for 2 days under in vitro decidualization. (B) Western blot analysis and quantification of BMP2 and SNAIL protein levels in stromal cells treated with Kyn for 2 days. (C) AhR fluorescence in stromal cells treated with 1 mM Kyn with or without 10 μM CH223191 for 24 h. Nuclei were counter-stained with DAPI. Scale bar, 50 μm. (D) The mRNA levels of Cyp1a1 and Cyp1b1 after stromal cells were treated with Kyn for 2 days under in vitro decidualization. All images are the representative of at least three biologically independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1622998/full'>41059340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-fcell-13-1622998-g004.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®P 4 activates AhR pathway. (A) Uterine phosphorylated AhR immunofluorescence after ovariectomized mice were treated with 2 mg or 4 mg P 4 for 7 days. Nuclei were counter-stained with PI. Le, luminal epithelia; St, stroma. Scale bar, 20 μm. n = 3 mice per group. (B) Uterine mRNA levels of Cyp1a1 and Cyp1b1 after ovariectomized mice were treated with 2, 4 or 8 mg P 4 for 7 days. (C) Western blot analysis and quantification of uterine CYP1A1 (3 days injection) and CYP1B1 (7 days injection) protein levels after ovariectomized mice were treated with 2, 4 or 8 mg P 4 . (D) Western blot analysis of AhR protein level in nuclear and cytoplasmic fractions, and quantification of AhR in nuclear fractions after stromal cells were treated with P 4 for 48 h (E) AhR immunofluorescence in stromal cells treated with 0.8, 4, or 20 μM P 4 for 48 h. Nuclei were counterstained with DAPI. Scale bar: 50 μm. All images are the representative of at least three biologically independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1622998/full'>41059340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-fcell-13-1622998-g005.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®Kyn inhibits mouse stromal cell decidualization through activating AhR. (A) The mRNA levels of Cyp1a1 and Cyp1b1 after stromal cells under in vitro decidualization were treated with Kyn for 48 h with or without AhR inhibitor CH223191. (B) Prl8a2 mRNA level after stromal cells under in vitro decidualization were treated with Kyn for 24 h with or without CH223191. (C) The mRNA levels of Prl8a2 and Prl3c1 after stromal cells were treated with 2-OH-E 2 for 12 h under in vitro decidualization. (D) The mRNA levels of Prl8a2 and Prl3c1 after stromal cells were treated with 4-OH-E 2 for 24 h under in vitro decidualization. All images are the representative of at least three biologically independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2025.1622998/full'>41059340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-wb-testing-1_2_1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®Western blot analysis of AHR using anti-AHR antibody (A00225-4). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human MCF-7 whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: mouse NIH/3T3 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (A00225-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AHR at approximately 100 kDa. The expected band size for AHR is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-ihc-testing-1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®IHC analysis of AHR using anti-AHR antibody (A00225-4). <br>
AHR was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-ihc-testing-2_2_1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®IHC analysis of AHR using anti-AHR antibody (A00225-4). <br>
AHR was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-ihc-testing-3_2_1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®IHC analysis of AHR using anti-AHR antibody (A00225-4). <br>
AHR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-ihc-testing-4_1_1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®IHC analysis of AHR using anti-AHR antibody (A00225-4). <br>
AHR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHR Antibody (A00225-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00225-4-ahr-primary-antibodies-if-testing-1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband®IF analysis of AHR using anti-AHR antibody (A00225-4) and anti-Tubulin Alpha antibody (M03989-3).<br>
AHR was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AHR Antibody (A00225-4) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-akr1d1-picoband-trade-antibody-a05278-2-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-2-akr1d1-primary-antibodies-wb-testing-1.jpgAnti-AKR1D1 Antibody Picoband® Western blot analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: mouse liver tissue lysates, <br>
Lane 3: human caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1D1 antigen affinity purified polyclonal antibody (Catalog # A05278-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1D1 at approximately 37KD. The expected band size for AKR1D1 is at 37KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-2-akr1d1-primary-antibodies-ihc-testing-2.jpgAnti-AKR1D1 Antibody Picoband® IHC analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-2). <br>
AKR1D1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1D1 Antibody (A05278-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-2-akr1d1-primary-antibodies-if-testing-3.jpgAnti-AKR1D1 Antibody Picoband® IF analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-2). <br>
AKR1D1 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-AKR1D1 Antibody (A05278-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05278-2-akr1d1-primary-antibodies-fc-testing-4.pngAnti-AKR1D1 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-AKR1D1 antibody (A05278-2). <br>Overlay histogram showing CACO-2 cells stained with A05278-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1D1 Antibody (A05278-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-alkbh1-antibody-a06945-1-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06945-1-alkbh1-primary-antibodies-wb-testing-1_1.jpgAnti-ALKBH1 Antibody Picoband®Western blot analysis of ALKBH1 using anti-ALKBH1 antibody (A06945-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates,<br>
Lane 2: human PC-3 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human K562 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH1 antigen affinity purified polyclonal antibody (A06945-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALKBH1 at approximately 40 kDa. The expected band size for ALKBH1 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06945-1-alkbh1-primary-antibodies-if-testing-1.jpgAnti-ALKBH1 Antibody Picoband®IF analysis of ALKBH1 using anti-ALKBH1 antibody (A06945-1) and anti-Tubulin Alpha antibody (M03989-3).<br>
ALKBH1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALKBH1 Antibody (A06945-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-alkbh5-picoband-trade-antibody-a03360-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig1_html.pngAnti-ALKBH5 Antibody Picoband®Comparison of human samples through immunohistochemical detection of ALKBH5 expression. ( * P < 0.05 indicates a significant difference between the indicated groups). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig2_html.pngAnti-ALKBH5 Antibody Picoband®( A ) qRT-PCR and Western blot were used to detect the expression of ALKBH5 to screen PTC cells. ( * P < 0.05 indicates a significant difference between each group compared to the Nthy-ori3-1 group). ( B ) Subcellular Localization of ALKBH5 Protein Detected by Immunofluorescence. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig3a_html.pngAnti-ALKBH5 Antibody Picoband®Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig3b_html.pngAnti-ALKBH5 Antibody Picoband®Transfection validation of ALKBH5 overexpression and interference ( A . IHH4 cells; B . TPC-1 cells). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig4a_html.pngAnti-ALKBH5 Antibody Picoband®Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig4b_html.pngAnti-ALKBH5 Antibody Picoband®Proliferation and invasion of IHH4 and TPC-1 Cells Detected by CCK-8 Assay and Transwell Assay ( A . CCK-8 assay to detect the cell viability of IHH4 and TPC-1 cells; B . Transwell assay to assess the invasion of IHH4 cells; C . Transwell assay to assess the invasion of TPC-1 cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-41598_2024_84352_fig5_html.pngAnti-ALKBH5 Antibody Picoband®m6A Methylation detection (A for IHH4 Cells; B for TPC-1 Cells. * P < 0.05 indicates a significant difference between each group compared to the OE-NC group; # P < 0.05 indicates a significant difference between each group compared to the OE-ALKBH5 group; & P < 0.05 indicates a significant difference between each group compared to the Si-NC group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-84352-w'>39789120</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-alkbh5-primary-antibodies-wb-testing-1_1.jpgAnti-ALKBH5 Antibody Picoband® Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human Hela whole cell lysates, <br>
Lane 4: human HepG2 whole cell lysates, <br>
Lane 5: human U87 whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog # A03360) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52 kDa. The expected band size for ALKBH5 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03360-alkbh5-primary-antibodies-if-testing-2.jpgAnti-ALKBH5 Antibody Picoband® IF analysis of ALKBH5 using anti-ALKBH5 antibody (A03360) and anti-Tubulin Alpha antibody (M03989-3).<br>
ALKBH5 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALKBH5 Antibody (A03360) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-androgen-receptor-ar-picoband-trade-antibody-a00542-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-ar-primary-antibodies-wb-testing-1.jpgAnti-Androgen Receptor/AR Antibody Picoband® Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human U20S whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human 22RV1 whole cell lysates, <br>
Lane 6: human HepG2 whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-zoolstud-57-041-g006.jpgAnti-Androgen Receptor/AR Antibody Picoband®Immuno-expression of AR (A:3.5yr.) and ERβ (B: 0.1yr., C: 3.5yr.
and D: 8yr.) in testes. Scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6517756/&orig_host=www.ncbi.nlm.nih.gov'>31966281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig1_html.pngAnti-Androgen Receptor/AR Antibody Picoband®Male mice were more susceptive to IRI and tubular apoptosis in kidney. A Experimental design. Female and male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in four groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls in male group ( n = 5). C BUN levels in four groups, as indicated. BUN was expressed as milligrams per deciliter. * P < 0.05 versus sham controls in male group ( n = 5). D Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to periodic acid–Schiff (PAS) staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL assay. Arrows indicate positive staining. Scale bar, 50 μm. E – I Representative western blot ( E ) and graphical representations of ( F ) FAS-L, ( G ) Bax, ( H ) cleaved caspase-3 and ( I ) KIM-1 protein expression levels are shown. * P < 0.05, ** P < 0.01 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig2_html.pngAnti-Androgen Receptor/AR Antibody Picoband®Sirtuin 6 is the possible contributor to gender differences upon IRI. A Graphical representations show the relative abundance of Sirtuin 1-7 mRNA in different groups. ** P < 0.01, *** P < 0.001 versus sham controls in male group ( n = 5); † P < 0.05 versus sham controls in female group ( n = 5). B , C Representative western blot ( B ) and graphical representations of ( C ) Sirtuin 1-7 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus male group ( n = 5). D Representative micrographs showing the expression of Sirtuin 6 in different groups, as indicated. Paraffin-embedded kidney sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. E Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. F – H Representative western blot ( F ) and graphical representations of ( G ) PGC-1α and ( H ) TOMM20 protein expression levels are shown. *** P < 0.001 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig4_html.pngAnti-Androgen Receptor/AR Antibody Picoband®The expression of Sirtuin 6 was the key regulator for gender differences in rhabdomyolysis-induced AKI. A , B Representative western blot ( A ) and graphical representations of ( B ) Sirtuin 1-7 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus female group ( n = 5). C Representative micrographs show the expression of Sirtuin 6 in different groups, as indicated. Paraffin-embedded kidney sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. D Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. E – G Representative western blot ( E ) and graphical representations of ( F ) PGC-1α and ( G ) TOMM20 protein expression levels are shown. * P < 0.05, *** P < 0.001 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig3_html.pngAnti-Androgen Receptor/AR Antibody Picoband®Male mice were more susceptive to rhabdomyolysis-induced AKI and tubular apoptosis in kidney. A Experimental design. Female and male mice were intramuscularly injected with 50% glycerol at the dose of 7.5 ml/kg or normal saline respectively. Mice were euthanized 3 days after intramuscular injection. B Scr levels in four groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls in male group ( n = 5); †† P < 0.01 versus sham controls in female group ( n = 5); # P < 0.05 versus male group in glycerol group ( n = 5). C Graphical representations show three day-mortality in different genders after glycerol administration, as indicated. * P < 0.05 versus male group (n of male group = 20; n of female group = 17). D Representative micrographs show renal tubular morphologic injury and the expression of KIM-1 in different groups, as indicated. Paraffin sections were subjected to PAS staining and stained with an antibody against KIM-1. Arrows indicate positive staining. Scale bar, 50 μm. E Tubular injury score depending on PAS staining in four groups, as indicated. *** P < 0.001 versus sham controls in male group ( n = 5); ††† P < 0.001 versus sham controls in female group ( n = 5). F – J Representative western blot ( F ) and graphical representations of ( G ) KIM-1, ( H ) FAS-L, ( I ) Bax and ( J ) cleaved caspase-3 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus female group ( n = 5). K Representative micrographs show the expression of caspase-3 and TUNEL staining in different groups, as indicated. Paraffin sections were stained with an antibody against caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrow indicates positive staining. Scale bar, 50 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-zoolstud-57-041-g002.jpgAnti-Androgen Receptor/AR Antibody Picoband®Growth layer groups (GLGs) in the thin section of a tooth. One
GLG consists of an opaque layer and a translucent layer. The arrow represents the
neonatal line. Scale bar = 200 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6517756/&orig_host=www.ncbi.nlm.nih.gov'>31966281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig5_html.pngAnti-Androgen Receptor/AR Antibody Picoband®The ectopic knockdown of AR ameliorates renal injury and mitochondrial dysfunction upon IRI. A Experimental design. Green arrow showed the injection of control-shRNA (pLVX-shRNA) or AR-shRNA (pLVX-shAR) plasmid. Male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in three groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). C BUN levels in three groups, as indicated. BUN was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls ( n = 5); † P < 0.05 versus control-shRNA group ( n = 5). D , E Representative western blot ( D ) and graphical representations of ( E ) AR protein expression levels are shown. *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). F , G Representative western blot ( F ) and graphical representations of ( G ) Sirtuin 6 protein expression levels are shown. ** P < 0.01 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). H Representative micrographs showing the expression of Sirtuin 6 in different groups. Paraffin sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. I Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to PAS staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrows indicate positive staining. Scale bar, 50 μm. (J – N) Representative western blot ( J ) and graphical representations of ( K ) KIM-1, ( L ) FAS-L, ( M ) Bax and ( N ) cleaved caspase-3 protein expression levels are shown. ** P < 0.01, *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01, ††† P < 0.001 versus control-shRNA group ( n = 5). O Tubular injury scores depending on PAS staining in three groups, as indicated. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus control-shRNA group ( n = 5). P Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. Q – S Representative western blot ( Q ) and graphical representations of ( R ) PGC-1α and ( S ) TOMM20 protein expression levels are shown. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus control-shRNA group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig6_html.pngAnti-Androgen Receptor/AR Antibody Picoband®The ectopic expression of Sirtuin 6 relieves renal injury and mitochondrial dysfunction upon IRI. A Experimental design. Green arrow showed the injection of pcDNA plasmid or pFlag-Sirtuin 6 overexpression plasmid. Male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in three groups, as indicated. Scr was expressed as milligrams per deciliter. *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01 versus pcDNA group ( n = 5). C Representative western blot of flag tag is shown. D Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. E – G Representative western blot ( E ) and graphical representations of ( F ) PGC-1α and ( G ) TOMM20 protein expression levels are shown. * P < 0.05 versus sham controls ( n = 5); †† P < 0.01, ††† P < 0.001 versus pcDNA group ( n = 5). H Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to periodic acid–Schiff (PAS) staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrows indicate positive staining. Scale bar, 50 μm. I – M Representative western blot ( I ) and graphical representations of ( J ) KIM-1, ( K ) FAS-L, ( L ) Bax and ( M ) cleaved caspase-3 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus sham controls ( n = 5); † P < 0.05, †† P < 0.01 versus pcDNA group ( n = 5). N Tubular injury score depending on PAS staining in three groups, as indicated. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus pcDNA group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-zoolstud-57-041-g003.jpgAnti-Androgen Receptor/AR Antibody Picoband®Histological sections of testes from birth to adulthood. LC:
Leydig cells; SC: Sertoli cells; PG: Primordial germ cells; SG: Spermatogonia; PS:
Primary spermatocytes; SS: Secondary spermatocytes; SPZ: Spermatozoa. (A) 0.1yr. (B)
2.5yr. (C) 3yr. (D) 3.5yr. (E) 4.5yr. (F) 6yr. (G) 8yr. (H) 13yr. Scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6517756/&orig_host=www.ncbi.nlm.nih.gov'>31966281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig7_html.pngAnti-Androgen Receptor/AR Antibody Picoband®AR increases acetylation of PGC-1α by downregulating Sirtuin 6 expression. A – C Representative western blot ( A ) and graphical representations of ( B ) Sirtuin 1-7 and ( C ) PGC-1α protein expression levels are shown. * P < 0.05, ** P < 0.01 versus control group ( n = 3). HKC-8 cells were transfected with pcDNA3 or AR overexpression plasmid for 24 h. D Representative ChIP assay results showing the binding of AR to the Sirtuin 6 gene promoter region. HKC‐8 cells were incubated with DHT (10 μMol/L) or not for 24 h. Cell lysates were precipitated with an antibody against AR, histone H3, or nonimmune IgG, and the ChIP assay was performed for Sirtuin 6 gene promoters. Total diluted lysate was used as the total genomic input DNA. E Graphical representations show the relative abundance of Sirtuin 1-7 mRNA in different groups. ** P < 0.01 versus control group ( n = 3). F – I Representative western blot ( F ) and graphical representations of ( G ) Sirtuin 1-7, ( H ) PGC-1α and ( I ) AR protein expression levels are shown. HKC-8 cells were incubated with DHT (10 μMol/L) and transfected with AR-shRNA for 24 h. * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3); † P < 0.05, †† P < 0.01, ††† P < 0.001 ( n = 3). J Representative graphs show the binding of PGC-1α with Sirtuin 6 or acetyl. HKC-8 cells were transfected with pcDNA3 or AR overexpression plasmid for 24 h. K Representative graphs show the binding of PGC-1α with acetyl, and the expression of PGC-1α in different groups, as indicated. HKC-8 cells were treated with DHT (10 μMol/L) and transfected with Sirtuin 6 overexpression plasmid for 24 h. L Representative graphs show the binding of PGC-1α with acetyl, and the protein levels of AR in nuclear fractions in different groups, as indicated. HKC-8 cells were treated with DHT (10 μMol/L) for 24 h. M Representative graphs show the binding of PGC-1α with acetyl, and the protein levels of AR in nuclear fractions in sham control and IRI group in male mice, as indicated. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-zoolstud-57-041-g005.jpgAnti-Androgen Receptor/AR Antibody Picoband®Immunoexpression of testosterone (A: 0.1yr, B: 3.5yr and C: 8yr)
and estradiol (D: 0.1yr, E: 3.5yr and F: 8yr) in testes from birth to adulthood. Upper
insert on panel A: negative control. Black arrows represent spermatozoa. Scale bar =
50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6517756/&orig_host=www.ncbi.nlm.nih.gov'>31966281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41420_2023_1432_fig8_html.pngAnti-Androgen Receptor/AR Antibody Picoband®Sirtuin 6 plays a key role in AR-induced mitochondrial dysfunction and tubular cell apoptosis in vitro. A – D Representative western blot ( A ) and graphical representations of ( B ) Bax, ( C ) cleaved caspase-3 and ( D ) PGC-1α protein expression levels are shown. * P < 0.05, ** P < 0.01 versus control group ( n = 3); † P < 0.05, ††† P < 0.001 versus STS group ( n = 3). HKC-8 were treated with STS (1 μMol/L) alone or co-treated with DHT (10 μMol/L) for 10 h. E , F TUNEL assay ( E ) and quantitative data ( F ) showed the degree of cellular apoptosis in different groups, as indicated. Frozen kidney sections were stained by TUNEL staining. Arrows indicate positive staining. Scale bar, 75 μm. *** P < 0.001 versus control group ( n = 3); ††† P < 0.001 versus STS group ( n = 3). G – L Representative western blot ( G ) and graphical representations of ( H ) FAS-L, ( I ) Bax, ( J ) cleaved caspase-3, ( K ) Sirtuin 6 and ( L ) PGC-1α protein expression levels are shown. HKC-8 is transfected with control-shRNA or AR-shRNA before H/R injury. * P < 0.05, ** P < 0.01, *** P < 0.001 versus control-shRNA group ( n = 3); † P < 0.05, †† P < 0.01, ††† P < 0.001 versus control-shRNA/H/R group ( n = 3). M – P Representative western blot ( M ) and graphical representations of ( N ) Bax, ( O ) cleaved caspase-3 and ( P ) PGC-1α protein expression levels are shown. HKC-8 cells were transfected with Sirtuin 6 overexpression plasmid and treated with STS (1 μMol/L) or co-treated with DHT (10 μMol/L) for 6 h. * P < 0.05, ** P < 0.01 ( n = 3); †† P < 0.01, ††† P < 0.001 ( n = 3). Q Androgen binds to the AR in cytoplasm, contributing to the translocation of AR from the cytoplasm into cell nucleus. The AR binds to Sirtuin 6 promoter and deregulates Sirtuin 6 expression. The decreased expression of Sirtuin 6 leads to decreased deacetylation of PGC-1α, contributing to decreased transcriptional activity of PGC-1α. It results in downregulation of mitochondrial biogenesis relative genes, such as TFAM, contributing to the imbalance of mitochondrial homeostasis, which further results in renal TECs apoptosis after AKI. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-medscimonit-24-2294-g001.jpgAnti-Androgen Receptor/AR Antibody Picoband®ABDHFA inhibits bladder cancer cell viability. For analysis of UMUC3 and TCCSUP cell viability after ABDHFA treatment, the MTT assay was used. GraphPad Prism software was used for constructing the bar graphs representing cell viability. Control cells were treated with dimethyl sulfoxide alone; * P<0.01 and ** P<0.02 vs. the cells treated with dimethyl sulfoxide alone.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5918918/&orig_host=www.ncbi.nlm.nih.gov'>29659560</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-medscimonit-24-2294-g002.jpgAnti-Androgen Receptor/AR Antibody Picoband®In AR-positive bladder cancer cells, ABDHFA treatment caused cell cycle arrest. Distribution of UMUC3 and TCCSUP bladder cancer cells in cell cycle was determined by flow cytometry. Western blot assay was used for analysis of expression of protein (cyclin D1) involved in regulation of cell cycle. Control cells were treated with dimethyl sulfoxide alone and in the treatment group with 50 μM concentration of ABDHFA for 48 h.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5918918/&orig_host=www.ncbi.nlm.nih.gov'>29659560</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-medscimonit-24-2294-g004.jpgAnti-Androgen Receptor/AR Antibody Picoband®Activation of AR in UMUC3 and TCCSUP cells was inhibited by ABDHFA. AR mRNA expression in bladder cancer cells was inhibited by treatment with ABDHFA for 48 h compared to control cells. The level of mRNA corresponding to PSA was also markedly reduced by ABDHFA treatment, as revealed by real-time PCR. Western blot assay confirmed that ABDHFA treatment markedly reduced expression of AR protein. * P<0.002 and ** P<0.001 vs. cells treated with DMSO alone.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5918918/&orig_host=www.ncbi.nlm.nih.gov'>29659560</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-medscimonit-24-2294-g005.jpgAnti-Androgen Receptor/AR Antibody Picoband®Up-regulation of miR-449a expression by ABDHFA treatment targeted AR expression. ABDHFA promoted miR-449a expression in TCCSUP and TCCSUP bladder cancer cells and inhibited AR level. Transfection of lentivirus markedly increased miR-449a expression in bladder cancer cells, which subsequently reduced PSA level. The miR-449a up-regulation by lentivirus reduced expression of AR protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5918918/&orig_host=www.ncbi.nlm.nih.gov'>29659560</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig2_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®Histological analysis of dorsolateral prostate in male aged rats treated with BPA for 3 months. The glandular cavity was slightly enlarged and increased in BPA-treated groups. ( a – d ) Representative sections of comparable regions were shown for vehicle control rats ( a ), and animals exposed to BPA (10 μg/kg/day) ( b ), BPA (30 μg/kg/day) ( c ), and BPA (90 μg/kg/day), ( d ) (scale bar: 50 μm, x40). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig3_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®( a ) Effect on height of dorsolateral prostatic epithelium. After aged rats were treated with 10–90 μg/kg BPA for 3 months, BPA significantly increased the height of DLP epithelium in a dose-dependent way, * P < 0.01, compared with the vehicle controls. BPA: bisphenol A. ( b ) The expression of the PCNA in DLP. The expression of PCNA was increased obviously in BPA-treated groups. * P < 0.05: compared with the vehicle controls; * P < 0.01, compared with the vehicle controls. BPA: bisphenol A. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig4_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®Immunohistochemical analysis of PCNA in DLP. Representative sections of comparable regions were shown for vehicle control rats ( a ), and animals exposed to BPA (10 μg/kg/day) ( b ), BPA (30 μg/kg/day) ( c ), and BPA (90 μg/kg/day), ( d ) (scale bar: 20 μm, ×400). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig5_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®Effects of BPA on E 2 , T, and Insulin serum levels in aged male rats. After aged rats were treated with 10–90 μg/kg BPA for 3 months, 90 μg/kg BPA significantly increased the E 2 level and the estrogen to androgen ratio; BPA had the trend of decreasing the T level and increasing the insulin level. p < 0.05, compared with the vehicle controls. BPA: bisphenol A. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig6_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®Clustering analysis and scatter plot of microarray data. ( a ) Clustering analysis; ( b ) scatter plot; Group1: dorsolateral prostate of the BPA group; Group control: dorsolateral prostate of the control group. BPA: bisphenol A. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-41598_2017_18869_fig7_html.jpgAnti-Androgen Receptor/AR Antibody Picoband®Immunohistochemical analysis of dorsolateral prostate E-cadherin, Vimtein, ERα and AR expression in aged rats. The expression of vimentin, ERα and AR increased, and the expression of E-cadherin decreased in BPA-treated groups. ( a – p ) Representative sections of comparable regions are shown for vehicle control rats ( a , e , i , m ), and animals exposed to BPA (10 μg/kg/day) ( b , f , j , n ), BPA (30 μg /kg/day) ( c , g , k , o ), and BPA (90 μg/kg/day) ( d , h , l , p ) (scale bar: 50 μm, ×400). BPA: bisphenol A; AR: androgen receptor; ERα:estrogen receptor-α. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-ar-primary-antibodies-fcm-testing-2.pngAnti-Androgen Receptor/AR Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-Androgen Receptor/AR antibody (A00542). <br>Overlay histogram showing A549 cells stained with A00542 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-ar-primary-antibodies-fcm-testing-3.pngAnti-Androgen Receptor/AR Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Androgen Receptor/AR antibody (A00542). <br>Overlay histogram showing C6 cells stained with A00542 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-ar-primary-antibodies-fcm-testing-4.pngAnti-Androgen Receptor/AR Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Androgen Receptor/AR antibody (A00542). <br>Overlay histogram showing RAW264.7 cells stained with A00542 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Androgen Receptor/AR Antibody (A00542, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00542-ar-primary-antibodies-if-testing-5.jpgAnti-Androgen Receptor/AR Antibody Picoband® IF analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). <br>
Androgen Receptor/AR was detected in immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Androgen Receptor/AR Antibody (A00542) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd239-bcam-picoband-trade-antibody-a03148-1-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03148-1-bcam-primary-antibodies-wb-testing-1.jpgAnti-CD239/BCAM Antibody Picoband® Western blot analysis of CD239/BCAM using anti-CD239/BCAM antibody (A03148-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human SK-OV-3 whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: rat kidney tissue lysates, <br>
Lane 6: mouse heart tissue lysates, <br>
Lane 7: mouse lung tissue lysates, <br>
Lane 8: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD239/BCAM antigen affinity purified polyclonal antibody (Catalog # A03148-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD239/BCAM at approximately 70KD. The expected band size for CD239/BCAM is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03148-1-bcam-primary-antibodies-ihc-testing-3.jpgAnti-CD239/BCAM Antibody Picoband® IHC analysis of BCAM using anti-BCAM antibody (A03148-1). <br>BCAM was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAM Antibody (A03148-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03148-1-bcam-primary-antibodies-ihc-testing-2.jpgAnti-CD239/BCAM Antibody Picoband® IHC analysis of CD239/BCAM using anti-CD239/BCAM antibody (A03148-1). <br>
CD239/BCAM was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD239/BCAM Antibody (A03148-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03148-1-bcam-primary-antibodies-if-testing-3.jpgAnti-CD239/BCAM Antibody Picoband® IF analysis of CD239/BCAM using anti-CD239/BCAM antibody (A03148-1). <br>
CD239/BCAM was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD239/BCAM Antibody (A03148-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03148-1-bcam-primary-antibodies-fcm-testing-4.pngAnti-CD239/BCAM Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-CD239/BCAM antibody (A03148-1). <br>Overlay histogram showing U20S cells stained with A03148-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD239/BCAM Antibody (A03148-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-brd2-picoband-trade-antibody-a02412-1-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02412-1-fphar-12-636154-g001.jpgAnti-BRD2 Antibody Picoband®I-BET151 reduces Brd4 levels in the kidney of HN rats. (A) A rat model of HN was established and treated with I-BET151 as indicated in “Material and Methods.” After 3 weeks, kidneys were taken for immunoblot analysis for Brd2, Brd3, Brd4 or GAPDH. Expression levels of Brd2 (B) , Brd3 (C) , Brd4 (D) were quantified by densitometry analysis and then normalized with GAPDH. (E) Photomicrographs (original magnification, ×400) illustrate immunohistochemical Brd4 staining of kidney tissues. (F) Brd4 staining graphic presentation of quantitative data. Data are represented as the mean ± SEM. * p < 0.05; ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.636154/full'>33664670</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02412-1-brd2-primary-antibodies-fcm-testing-3.jpgAnti-BRD2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-BRD2 antibody (A02412-1). <br>Overlay histogram showing 293T cells stained with A02412-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD2 Antibody (A02412-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02412-1-brd2-primary-antibodies-wb-testing-1_1.jpgAnti-BRD2 Antibody Picoband® Western blot analysis of BRD2 using anti-BRD2 antibody (A02412-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human A431 whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: human 293T whole cell lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD2 antigen affinity purified polyclonal antibody (Catalog # A02412-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD2 at approximately 110 kDa. The expected band size for BRD2 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02412-1-brd2-primary-antibodies-if-testing-2_1.jpgAnti-BRD2 Antibody Picoband® IF analysis of BRD2 and Tubulin beta using anti-BRD2 antibody (A02412-1) and anti-Tubulin beta antibody (M05613-4). <br>
BRD2 and Tubulin beta was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BRD2 Antibody (A02412-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-calretinin-calb2-picoband-trade-antibody-a04255-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-1.jpgAnti-Calretinin/CALB2 Antibody Picoband® IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>
Calretinin/CALB2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-5.jpgAnti-Calretinin/CALB2 Antibody Picoband®IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>Calretinin/CALB2 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-2.jpgAnti-Calretinin/CALB2 Antibody Picoband® IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>
Calretinin/CALB2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-4.jpgAnti-Calretinin/CALB2 Antibody Picoband®IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>Calretinin/CALB2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-3.jpgAnti-Calretinin/CALB2 Antibody Picoband® IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>
Calretinin/CALB2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-cabl2-primary-antibodies-wb-testing-6.jpgAnti-Calretinin/CALB2 Antibody Picoband® Western blot analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A00346). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calretinin/CALB2 antigen affinity purified polyclonal antibody (Catalog # A00346) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calretinin/CALB2 at approximately 32KD. The expected band size for Calretinin/CALB2 is at 32KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-cabl2-primary-antibodies-if-testing-7.jpgAnti-Calretinin/CALB2 Antibody Picoband® IF analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A00346). <br>
Calretinin/CALB2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Calretinin/CALB2 Antibody (A00346) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-6.jpgAnti-Calretinin/CALB2 Antibody Picoband®IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>Calretinin/CALB2 was detected in a paraffin-embedded section of human cerebral cortical tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-ihc-testing-7.jpgAnti-Calretinin/CALB2 Antibody Picoband®IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (A04255). <br>Calretinin/CALB2 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (A04255) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-fcm-testing-4.pngAnti-Calretinin/CALB2 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Calretinin/CALB2 antibody (A04255). <br>Overlay histogram showing C6 cells stained with A04255 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calretinin/CALB2 Antibody (A04255, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04255-calb2-primary-antibodies-fcm-testing-5.pngAnti-Calretinin/CALB2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Calretinin/CALB2 antibody (A04255).<br>Overlay histogram showing A431 cells stained with A04255 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calretinin/CALB2 Antibody (A04255, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-camkii-alpha-camk2a-picoband-trade-antibody-a03241-2-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2a-primary-antibodies-wb-testing-1.jpgAnti-CaMKII alpha/CAMK2A Antibody Picoband® Western blot analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat brain tissue lysates, <br>
Lane 3: mouse brain tissue lysates, <br>
Lane 4: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha/CAMK2A antigen affinity purified polyclonal antibody (Catalog # A03241-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII alpha/CAMK2A at approximately 54KD. The expected band size for CaMKII alpha/CAMK2A is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2a-primary-antibodies-fcm-testing-2_1.pngAnti-CaMKII alpha/CAMK2A Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>Overlay histogram showing U87 cells stained with A03241-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (A03241-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2a-primary-antibodies-fcm-testing-3.pngAnti-CaMKII alpha/CAMK2A Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>Overlay histogram showing ANA-1 cells stained with A03241-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (A03241-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2a-primary-antibodies-fcm-testing-4.pngAnti-CaMKII alpha/CAMK2A Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>Overlay histogram showing NRK cells stained with A03241-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (A03241-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2-primary-antibody-if-testing-5.jpgAnti-CaMKII alpha/CAMK2A Antibody Picoband® IF analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>
CaMKII alpha/CAMK2A was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CaMKII alpha/CAMK2A Antibody (A03241-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03241-2-camk2-primary-antibody-if-testing-6.jpgAnti-CaMKII alpha/CAMK2A Antibody Picoband® IF analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (A03241-2). <br>
CaMKII alpha/CAMK2A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CaMKII alpha/CAMK2A Antibody (A03241-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-b1-ccnb1-picoband-trade-antibody-a00745-1-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: human K562 whole cell lysates, <br>
Lane 6: human U2OS whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-wb-testing-2.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: mouse thymus tissue lysates, <br>
Lane 3: mouse NIH-3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-wb-testing-3.pngAnti-Cyclin B1/CCNB1 Antibody Picoband® Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: normal group-rat colon tissue lysates, <br>
Lane 2: model group- model rat colon tissue, <br>
Lane 3: low Chinese medicine group-model rat colon tissue,<br>
Lane 4: medium Chinese medicine group-model rat colon tissue,<br>
Lane 5: high Chinese medicine group-model rat colon tissue,<br>
Lane 6: western medicine group-model rat colon tissue.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. The expected band size for CCNB1 is at 55KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-3.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
CCNB1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-4.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
CCNB1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-5.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
CCNB1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-6.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1).<br>
CCNB1 was detected in paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-fc-testing-10.pngAnti-Cyclin B1/CCNB1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-CCNB1 antibody (A00745-1). <br>Overlay histogram showing A431 cells stained with A00745-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB1 Antibody (A00745-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-7.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1).<br>
CCNB1 was detected in paraffin-embedded section of human seminoma testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-ihc-testing-8.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IHC analysis of CCNB1 using anti-CCNB1 antibody (A00745-1).<br>
CCNB1 was detected in paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-1-ccnb1-primary-antibodies-if-testing-9.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband® IF analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). <br>
CCNB1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCNB1 Antibody (A00745-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-e1-ccne1-picoband-trade-antibody-a00543-2-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® Western blot analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human U2OS whole cell lysates, <br>
Lane 5: human A549 whole cell lysates, <br>
Lane 6: human PANC-1 whole cell lysates, <br>
Lane 7: human CACO-2 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (Catalog # A00543-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47KD. The expected band size for CCNE1 is at 47KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® IHC analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
CCNE1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNE1 Antibody (A00543-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-ihc-testing-3.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® IHC analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
CCNE1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNE1 Antibody (A00543-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-ihc-testing-4.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® IHC analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
CCNE1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNE1 Antibody (A00543-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-ihc-testing-5.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® IHC analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
CCNE1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNE1 Antibody (A00543-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-ihc-testing-6.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® IHC analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). <br>
CCNE1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCNE1 Antibody (A00543-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00543-2-ccne1-primary-antibodies-fcm-testing-7.pngAnti-Cyclin E1/CCNE1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CCNE1 antibody (A00543-2). <br>Overlay histogram showing SiHa cells stained with A00543-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNE1 Antibody (A00543-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cd79a-picoband-trade-antibody-a01047-1-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-wb-testing-1_1.jpgAnti-Cd79a Antibody Picoband® Western blot analysis of Cd79a using anti-Cd79a antibody (A01047-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: mouse spleen tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd79a antigen affinity purified polyclonal antibody (Catalog # A01047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd79a at approximately 25-30 kDa. The expected band size for Cd79a is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-ihc-testing-2.jpgAnti-Cd79a Antibody Picoband® IHC analysis of Cd79a using anti-Cd79a antibody (A01047-1). <br>
Cd79a was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cd79a Antibody (A01047-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-ihc-testing-3.jpgAnti-Cd79a Antibody Picoband® IHC analysis of Cd79a using anti-Cd79a antibody (A01047-1). <br>
Cd79a was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cd79a Antibody (A01047-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-if-testing-4.jpgAnti-Cd79a Antibody Picoband® IF analysis of Cd79a using anti-Cd79a antibody (A01047-1). <br>
Cd79a was detected in paraffin-embedded section of mouse intestine (lymph node) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cd79a Antibody (A01047-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-if-testing-5.jpgAnti-Cd79a Antibody Picoband® IF analysis of Cd79a using anti-Cd79a antibody (A01047-1). <br>
Cd79a was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cd79a Antibody (A01047-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01047-1-cd79a-primary-antibodies-fcm-testing-6.pngAnti-Cd79a Antibody Picoband® Flow Cytometry analysis of mouse spleen tissues using anti-Cd79a antibody (A01047-1). <br>Overlay histogram showing mouse spleen tissues stained with A01047-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd79a Antibody (A01047-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-cdr2-picoband-trade-antibody-a02973-2-boster.html2026-03-24T05:26:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02973-2-cdr2-primary-antibodies-wb-testing-1_1.jpgAnti-CDR2 Antibody Picoband® Western blot analysis of CDR2 using anti-CDR2 antibody (A02973-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human CACO-2 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR2 antigen affinity purified polyclonal antibody (Catalog # A02973-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR2 at approximately 62KD. The expected band size for CDR2 is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02973-2-cdr2-primary-antibodies-ihc-testing-2.jpgAnti-CDR2 Antibody Picoband® IHC analysis of CDR2 using anti-CDR2 antibody (A02973-2). <br>
CDR2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDR2 Antibody (A02973-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02973-2-cdr2-primary-antibodies-ihc-testing-3.jpgAnti-CDR2 Antibody Picoband® IHC analysis of CDR2 using anti-CDR2 antibody (A02973-2). <br>
CDR2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDR2 Antibody (A02973-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02973-2-cdr2-primary-antibodies-ihc-testing-4.jpgAnti-CDR2 Antibody Picoband® IHC analysis of CDR2 using anti-CDR2 antibody (A02973-2). <br>
CDR2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDR2 Antibody (A02973-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02973-2-cdr2-primary-antibodies-fcm-testing-5.pngAnti-CDR2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CDR2 antibody (A02973-2). <br>Overlay histogram showing HepG2 cells stained with A02973-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDR2 Antibody (A02973-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-clk2-picoband-trade-antibody-a05206-2-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05206-2-clk2-primary-antibodies-wb-testing-1_1.jpgAnti-CLK2 Antibody Picoband® Western blot analysis of CLK2 using anti-CLK2 antibody (A05206-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human HEL whole cell lysates,<br>
Lane 2: human SH-SY5Y whole cell lysates,<br>
Lane 3: human RT4 whole cell lysates,<br>
Lane 4: rat lung tissue lysates,<br>
Lane 5: rat brain tissue lysates,<br>
Lane 6: mouse lung tissue lysates,<br>
Lane 7: mouse brain tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLK2 antigen affinity purified polyclonal antibody (Catalog # A05206-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLK2 at approximately 60 kDa. The expected band size for CLK2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05206-2-clk2-primary-antibodies-fcm-testing-2.pngAnti-CLK2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CLK2 antibody (A05206-2). <br>Overlay histogram showing PC-3 cells stained with A05206-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLK2 Antibody (A05206-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-il-3rb-csf2rb-picoband-trade-antibody-a02219-3-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02219-3-csf2rb-primary-antibodies-wb-testing-1.jpgAnti-IL-3RB/Csf2rb Antibody Picoband® Western blot analysis of CSF2RB using anti-CSF2RB antibody (A02219-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse ANA-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF2RB antigen affinity purified polyclonal antibody (Catalog # A02219-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF2RB at approximately 120KD. The expected band size for CSF2RB is at 120KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02219-3-csf2rb-primary-antibodies-ihc-testing-2.jpgAnti-IL-3RB/Csf2rb Antibody Picoband® IHC analysis of CSF2RB using anti-CSF2RB antibody (A02219-3). <br>
CSF2RB was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSF2RB Antibody (A02219-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02219-3-csf2rb-primary-antibodies-fc-testing-3.pngAnti-IL-3RB/Csf2rb Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-CSF2RB antibody (A02219-3). <br>Overlay histogram showing ANA-1 cells stained with A02219-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSF2RB Antibody (A02219-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02219-3-csf2rb-primary-antibodies-fc-testing-4.pngAnti-IL-3RB/Csf2rb Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-CSF2RB antibody (A02219-3). <br>Overlay histogram showing NRK cells stained with A02219-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSF2RB Antibody (A02219-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ddit3-picoband-trade-antibody-a00311-2-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00311-2-ddit3-primary-antibodies-wb-testing-1.jpgAnti-DDIT3 Antibody Picoband®Western blot analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates,<br>
Lane 2: human U87 whole cell lysates,<br>
Lane 3: human MCF-7 whole cell lysates,<br>
Lane 4: rat C6 whole cell lysates,<br>
Lane 5: rat PC-12 whole cell lysates,<br>
Lane 6: mouse RAW264.7 whole cell lysates,<br>
Lane 7: mouse SP2/0 whole cell lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT3 antigen affinity purified polyclonal antibody (A00311-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDIT3 at approximately 29 kDa. The expected band size for DDIT3 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-2.jpgAnti-DDIT3 Antibody Picoband® IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). <br>
DDIT3 was detected in paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-3.jpgAnti-DDIT3 Antibody Picoband® IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). <br>
DDIT3 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00311-2-ddit3-primary-antibodies-ihc-testing-4.jpgAnti-DDIT3 Antibody Picoband® IHC analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). <br>
DDIT3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDIT3 Antibody (A00311-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00311-2-ddit3-primary-antibodies-fc-testing-5_1.pngAnti-DDIT3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-DDIT3 antibody (A00311-2). <br>Overlay histogram showing THP-1 cells stained with A00311-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDIT3 Antibody (A00311-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rna-helicase-a-dhx9-picoband-trade-antibody-a02550-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-wb-testing-1.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® Western blot analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: human K562 whole cell lysates, <br>
Lane 5: human Caco-2 whole cell lysates, <br>
Lane 6: human SW620 whole cell lysates, <br>
Lane 7: human Raji whole cell lysates, <br>
Lane 8: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX9 antigen affinity purified polyclonal antibody (Catalog # A02550-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX9 at approximately 141KD. The expected band size for DHX9 is at 141KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-10.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-wb-testing-2.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® Western blot analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat PC-12 whole cell lysates, <br>
Lane 2: mouse brain tissue lysates, <br>
Lane 3: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX9 antigen affinity purified polyclonal antibody (Catalog # A02550-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX9 at approximately 141KD. The expected band size for DHX9 is at 141KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-3.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-4.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-5.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-6.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-7.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-8.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ihc-testing-9.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IHC analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-if-testing-11.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IF analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-if-testing-12.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband® IF analysis of DHX9 using anti-DHX9 antibody (A02550-1). <br>
DHX9 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-DHX9 Antibody (A02550-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-ip-testing-1.jpgAnti-RNA Helicase A/DHX9 Antibody Picoband®Immunoprecipitating (IP) DHX9 in 293T whole cell lysate.<br>
Western blot analysis of DHX9 using anti-DHX9 antibody (A02550-1); <br>
Lane 1: 293T whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-DHX9 antibody in 293T whole cell lysate;<br>
Lane 3: anti-DHX9 antibody (2μg) + 293T whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DHX9 antigen affinity purified polyclonal antibody (A02550-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for DHX9 at approximately 141 kDa. The expected band size for DHX9 is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02550-1-dhx9-primary-antibodies-fc-testing-13_1.pngAnti-RNA Helicase A/DHX9 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-DHX9 antibody (A02550-1). <br>Overlay histogram showing HL-60 cells stained with A02550-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX9 Antibody (A02550-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-dio1-picoband-trade-antibody-a04612-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04612-dio1-primary-antibodies-wb-testing-1.jpgAnti-DIO1 Antibody Picoband® Western blot analysis of DIO1 using anti-DIO1 antibody (A04612). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIO1 antigen affinity purified polyclonal antibody (Catalog # A04612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIO1 at approximately 29KD. The expected band size for DIO1 is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04612-dio1-primary-antibodies-if-testing-2.jpgAnti-DIO1 Antibody Picoband® IF analysis of DIO1 using anti-DIO1 antibody (A04612). <br>
DIO1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DIO1 Antibody (A04612) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04612-dio1-primary-antibodies-fc-testing-3.pngAnti-DIO1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-DIO1 antibody (A04612). <br>Overlay histogram showing 293T cells stained with A04612 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIO1 Antibody (A04612, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04612-dio1-primary-antibodies-fc-testing-4.pngAnti-DIO1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-DIO1 antibody (A04612). <br>Overlay histogram showing ANA-1 cells stained with A04612 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIO1 Antibody (A04612, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-dnaja2-picoband-trade-antibody-a08918-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-wb-testing-1_1.jpgAnti-DNAJA2 Antibody Picoband®Western blot analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates,<br>
Lane 2: human A549 whole cell lysates,<br>
Lane 3: human Hela whole cell lysates,<br>
Lane 4: human Jurkat whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat lung tissue lysates,<br>
Lane 7: mouse kidney tissue lysates,<br>
Lane 8: mouse lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2 antigen affinity purified polyclonal antibody (A08918-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJA2 at approximately 46 kDa. The expected band size for DNAJA2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-ihc-testing-2.jpgAnti-DNAJA2 Antibody Picoband® IHC analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). <br>
DNAJA2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DNAJA2 Antibody (A08918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-ihc-testing-3.jpgAnti-DNAJA2 Antibody Picoband® IHC analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). <br>
DNAJA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DNAJA2 Antibody (A08918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-ihc-testing-4.jpgAnti-DNAJA2 Antibody Picoband® IHC analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). <br>
DNAJA2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DNAJA2 Antibody (A08918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-ihc-testing-5.jpgAnti-DNAJA2 Antibody Picoband® IHC analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). <br>
DNAJA2 was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DNAJA2 Antibody (A08918-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-fc-testing-6.pngAnti-DNAJA2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-DNAJA2 antibody (A08918-1). <br>Overlay histogram showing A431 cells stained with A08918-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJA2 Antibody (A08918-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-fc-testing-7.pngAnti-DNAJA2 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-DNAJA2 antibody (A08918-1). <br>Overlay histogram showing ANA-1 cells stained with A08918-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJA2 Antibody (A08918-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08918-1-dnaja2-primary-antibodies-fc-testing-8.pngAnti-DNAJA2 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-DNAJA2 antibody (A08918-1). <br>Overlay histogram showing NRK cells stained with A08918-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJA2 Antibody (A08918-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-eapp-picoband-trade-antibody-a07290-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-wb-testing-1.jpgAnti-EAPP Antibody Picoband® Western blot analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human COLO-320 whole cell lysates, <br>
Lane 4: human Jurkat whole cell lysates, <br>
Lane 5: human HEK293 whole cell lysates, <br>
Lane 6: human K562 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAPP antigen affinity purified polyclonal antibody (Catalog # A07290-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAPP at approximately 45KD. The expected band size for EAPP is at 45KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-ihc-testing-2.jpgAnti-EAPP Antibody Picoband® IHC analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
EAPP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EAPP Antibody (A07290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-ihc-testing-3.jpgAnti-EAPP Antibody Picoband® IHC analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
EAPP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EAPP Antibody (A07290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-ihc-testing-4.jpgAnti-EAPP Antibody Picoband® IHC analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
EAPP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EAPP Antibody (A07290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-ihc-testing-5.jpgAnti-EAPP Antibody Picoband® IHC analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
EAPP was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EAPP Antibody (A07290-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-if-testing-6.jpgAnti-EAPP Antibody Picoband® IF analysis of EAPP using anti-EAPP antibody (A07290-1). <br>
EAPP was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EAPP Antibody (A07290-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07290-1-eapp-primary-antibodies-fc-testing-7.pngAnti-EAPP Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-EAPP antibody (A07290-1). <br>Overlay histogram showing HepG2 cells stained with A07290-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EAPP Antibody (A07290-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-eps15-picoband-trade-antibody-a01681-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01681-1-eps15-primary-antibodies-wb-testing-1.jpgAnti-EPS15 Antibody Picoband® Western blot analysis of EPS15 using anti-EPS15 antibody (A01681-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates, <br>
Lane 3: mouse NIH-3T3 whole cell lysates, <br>
Lane 4: human Hela whole cell lysates, <br>
Lane 5: human Jurkat whole cell lysates, <br>
Lane 6: human HEK293 whole cell lysates, <br>
Lane 7: human PC-3 whole cell lysates, <br>
Lane 8: human THP-1 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 138KD. The expected band size for EPS15 is at 138KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01681-1-eps15-primary-antibodies-fc-testing-2.pngAnti-EPS15 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-EPS15 antibody (A01681-1). <br>Overlay histogram showing A431 cells stained with A01681-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPS15 Antibody (A01681-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fabp6-picoband-trade-antibody-a07132-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-wb-testing-1.jpgAnti-FABP6 Antibody Picoband® Western blot analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat intestine tissue lysates, <br>
Lane 2: rat intestine tissue lysates, <br>
Lane 3: mouse intestine tissue lysates, <br>
Lane 4: mouse intestine tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP6 antigen affinity purified polyclonal antibody (Catalog # A07132) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP6 at approximately 14KD. The expected band size for FABP6 is at 14KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-ihc-testing-2.jpgAnti-FABP6 Antibody Picoband® IHC analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
FABP6 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FABP6 Antibody (A07132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-ihc-testing-3.jpgAnti-FABP6 Antibody Picoband® IHC analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
FABP6 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FABP6 Antibody (A07132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-ihc-testing-4.jpgAnti-FABP6 Antibody Picoband® IHC analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
FABP6 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FABP6 Antibody (A07132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-ihc-testing-5.jpgAnti-FABP6 Antibody Picoband® IHC analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
FABP6 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FABP6 Antibody (A07132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-ihc-testing-6.jpgAnti-FABP6 Antibody Picoband® IHC analysis of FABP6 using anti-FABP6 antibody (A07132). <br>
FABP6 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FABP6 Antibody (A07132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07132-fabp6-primary-antibodies-fc-testing-7.pngAnti-FABP6 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-FABP6 antibody (A07132). <br>Overlay histogram showing 293T cells stained with A07132 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FABP6 Antibody (A07132, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-fgf10-picoband-trade-antibody-a01709-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01709-fgf10-primary-antibodies-wb-testing-1_1.jpgAnti-FGF10 Antibody Picoband® Western blot analysis of FGF10 using anti-FGF10 antibody (A01709). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat ovary tissue lysates, <br>
Lane 2: mouse lung tissue lysates, <br>
Lane 3: mouse ovary tissue lysates, <br>
Lane 4: mouse kidney tissue lysates, <br>
Lane 5: mouse NIH-3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF10 antigen affinity purified polyclonal antibody (Catalog # A01709) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF10 at approximately 20KD. The expected band size for FGF10 is at 20KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01709-fgf10-primary-antibodies-if-testing-2.jpgAnti-FGF10 Antibody Picoband® IF analysis of FGF10 using anti-FGF10 antibody (A01709). <br>
FGF10 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FGF10 Antibody (A01709) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01709-fgf10-primary-antibodies-fc-testing-3.pngAnti-FGF10 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-FGF10 antibody (A01709). <br>Overlay histogram showing Jurkat cells stained with A01709 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGF10 Antibody (A01709, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ttc11-fis1-picoband-trade-antibody-a01932-2-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-wb-testing-1.jpgAnti-TTC11/FIS1 Antibody Picoband® Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: rat kidney tissue lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse spleen tissue lysates, <br>
Lane 6: mouse kidney tissue lysates, <br>
Lane 7: mouse NIH-3T3 whole cell lysates, <br>
Lane 8: mouse RAW264.7 whole cell lysates, <br>
Lane 9: human HEK293 whole cell lysates, <br>
Lane 10: human A549 whole cell lysates, <br>
Lane 11: human Hela whole cell lysates, <br>
Lane 12: human Raji whole cell lysates, <br>
Lane 13: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-ihc-testing-2.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of FIS1 using anti-FIS1 antibody (A01932-2). <br>
FIS1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-ihc-testing-3.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of FIS1 using anti-FIS1 antibody (A01932-2). <br>
FIS1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-ihc-testing-4.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of FIS1 using anti-FIS1 antibody (A01932-2). <br>
FIS1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-fc-testing-5.jpgAnti-TTC11/FIS1 Antibody Picoband® IF analysis of FIS1 using anti-FIS1 antibody (A01932-2). <br>
FIS1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-2-fis1-primary-antibodies-fc-testing-6.pngAnti-TTC11/FIS1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-FIS1 antibody (A01932-2). <br>Overlay histogram showing A549 cells stained with A01932-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FIS1 Antibody (A01932-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-frmd6-willin-picoband-trade-antibody-a08826-2-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08826-2-frmd6-primary-antibodies-wb-testing-1.jpgAnti-FRMD6/Willin Antibody Picoband® Western blot analysis of FRMD6 using anti-FRMD6 antibody (A08826-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human PANC-1 whole cell lysates, <br>
Lane 4: human CACO-2 whole cell lysates, <br>
Lane 5: rat PC-12 whole cell lysates, <br>
Lane 6: mouse Neuro-2a whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRMD6 antigen affinity purified polyclonal antibody (Catalog # A08826-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRMD6 at approximately 72KD. The expected band size for FRMD6 is at 72KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08826-2-frmd6-primary-antibodies-ihc-testing-2.jpgAnti-FRMD6/Willin Antibody Picoband® IHC analysis of FRMD6 using anti-FRMD6 antibody (A08826-2). <br>
FRMD6 was detected in paraffin-embedded section of human rectum cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FRMD6 Antibody (A08826-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08826-2-frmd6-primary-antibodies-if-testing-3.jpgAnti-FRMD6/Willin Antibody Picoband® IF analysis of FRMD6 using anti-FRMD6 antibody (A08826-2). <br>
FRMD6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FRMD6 Antibody (A08826-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08826-2-frmd6-primary-antibodies-fc-testing-4.pngAnti-FRMD6/Willin Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-FRMD6 antibody (A08826-2). <br>
Overlay histogram showing A431 cells stained with A08826-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FRMD6 Antibody (A08826-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-gfi1-picoband-trade-antibody-a00888-3-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00888-3-gfi1-primary-antibodies-wb-testing-1.jpgAnti-GFI1 Antibody Picoband® Western blot analysis of GFI1 using anti-GFI1 antibody (A00888-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: mouse thymus tissue lysates, <br>
Lane 3: mouse RAW264.7 whole cell lysates, <br>
Lane 4: mouse ANA-1 whole cell lysates, <br>
Lane 5: human THP-1 whole cell lysates, <br>
Lane 6: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFI1 antigen affinity purified polyclonal antibody (Catalog # A00888-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFI1 at approximately 55KD. The expected band size for GFI1 is at 45KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00888-3-gfi1-primary-antibodies-ihc-testing-2.jpgAnti-GFI1 Antibody Picoband® IHC analysis of GFI1 using anti-GFI1 antibody (A00888-3). <br>
GFI1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFI1 Antibody (A00888-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00888-3-gfi1-primary-antibodies-fc-testing-3.pngAnti-GFI1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-GFI1 antibody (A00888-3). <br>Overlay histogram showing THP-1 cells stained with A00888-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GFI1 Antibody (A00888-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-hgs-picoband-trade-antibody-a01174-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-wb-testing-1.jpgAnti-HGS Antibody Picoband® Western blot analysis of HGS using anti-HGS antibody (A01174-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human SK-OV-3 whole cell lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: human A549 whole cell lysates, <br>
Lane 7: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (Catalog # A01174-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGS at approximately 110-115KD. The expected band size for HGS is at 110-115KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-wb-testing-2_1.jpgAnti-HGS Antibody Picoband® Western blot analysis of HGS using anti-HGS antibody (A01174-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat PC-12 whole cell lysates, <br>
Lane 3: rat NRK whole cell lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse HEPA1-6 whole cell lysates, <br>
Lane 6: mouse NIH-3T3 whole cell lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (Catalog # A01174-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGS at approximately 110-115KD. The expected band size for HGS is at 110-115KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-ihc-testing-3.jpgAnti-HGS Antibody Picoband® IHC analysis of HGS using anti-HGS antibody (A01174-1). <br>
HGS was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HGS Antibody (A01174-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-ihc-testing-4.jpgAnti-HGS Antibody Picoband® IHC analysis of HGS using anti-HGS antibody (A01174-1). <br>
HGS was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HGS Antibody (A01174-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-ihc-testing-5.jpgAnti-HGS Antibody Picoband® IHC analysis of HGS using anti-HGS antibody (A01174-1). <br>
HGS was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HGS Antibody (A01174-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-if-testing-6.jpgAnti-HGS Antibody Picoband® IF analysis of HGS using anti-HGS antibody (A01174-1). <br>
HGS was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HGS Antibody (A01174-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-if-testing-1.jpgAnti-HGS Antibody Picoband®Immunoprecipitating (IP) HGS in K562 whole cell lysate.<br>
Western blot analysis of HGS using anti-HGS antibody (A01174-1); <br>
Lane 1: K562 whole cell lysates (30ug);<br>
Lane 2: Rabbit control IgG instead of anti-HGS antibody in K562 whole cell lysate;<br>
Lane 3: anti-HGS antibody (2μg) + K562 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (A01174-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for HGS at approximately 100-110 kDa. The expected band size for HGS is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01174-1-hgs-primary-antibodies-fc-testing-7_1.pngAnti-HGS Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-HGS antibody (A01174-1). <br>Overlay histogram showing A549 cells stained with A01174-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HGS Antibody (A01174-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ifn-gamma-receptor-beta-af-1-ifngr2-picoband-trade-antibody-a03158-2-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03158-2-ifngr2-primary-antibodies-wb-testing-1.jpgAnti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody Picoband® Western blot analysis of IFN Gamma Receptor Beta/AF-1/Ifngr2 using anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antibody (A03158-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: mouse thymus tissue lysates, <br>
Lane 2: mouse lung tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antigen affinity purified polyclonal antibody (Catalog # A03158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN Gamma Receptor Beta/AF-1/Ifngr2 at approximately 55KD. The expected band size for IFN Gamma Receptor Beta/AF-1/Ifngr2 is at 38KD.
https://www.bosterbio.com/products/primary-antibodies/anti-ift88-picoband-trade-antibody-a06814-1-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-wb-testing-1.jpgAnti-IFT88 Antibody Picoband® Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates, <br>
Lane 2: mouse testis tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-ihc-testing-2.jpgAnti-IFT88 Antibody Picoband® IHC analysis of IFT88 using anti-IFT88 antibody (A06814-1). <br>
IFT88 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFT88 Antibody (A06814-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-ihc-testing-3.jpgAnti-IFT88 Antibody Picoband® IHC analysis of IFT88 using anti-IFT88 antibody (A06814-1). <br>
IFT88 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFT88 Antibody (A06814-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-ihc-testing-4.jpgAnti-IFT88 Antibody Picoband® IHC analysis of IFT88 using anti-IFT88 antibody (A06814-1). <br>
IFT88 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFT88 Antibody (A06814-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-if-testing-5.jpgAnti-IFT88 Antibody Picoband® IF analysis of IFT88 using anti-IFT88 antibody (A06814-1). <br>
IFT88 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IFT88 Antibody (A06814-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06814-1-ift88-primary-antibodies-fc-testing-6.pngAnti-IFT88 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-IFT88 antibody (A06814-1). <br>Overlay histogram showing THP-1 cells stained with A06814-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT88 Antibody (A06814-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-il3ra-cd123-picoband-trade-antibody-a04307-boster.html2026-03-24T05:26:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04307-il3ra-primary-antibodies-wb-testing-1_.jpgAnti-IL3RA/CD123 Antibody Picoband® Western blot analysis of IL3RA using anti-IL3RA antibody (A04307). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL3RA antigen affinity purified polyclonal antibody (Catalog # A04307) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL3RA at approximately 60KD. The expected band size for IL3RA is at 60KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04307-il3ra-primary-antibodies-ihc-testing-2.jpgAnti-IL3RA/CD123 Antibody Picoband® IHC analysis of IL3RA using anti-IL3RA antibody (A04307). <br>
IL3RA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL3RA Antibody (A04307) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04307-il3ra-primary-antibodies-fc-testing-3.pngAnti-IL3RA/CD123 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-IL3RA antibody (A04307). <br>Overlay histogram showing Raji cells stained with A04307 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL3RA Antibody (A04307, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ldl-receptor-ldlr-picoband-trade-antibody-a00076-2-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-2-ldlr-primary-antibodies-wb-testing-1.jpgAnti-LDL Receptor/LDLR Antibody Picoband® Western blot analysis of LDLR using anti-LDLR antibody (A00076-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Raji whole cell lysates, <br>
Lane 3: human U-87MG whole cell lysates, <br>
Lane 4: rat liver tissue lysates, <br>
Lane 5: rat lung tissue lysates, <br>
Lane 6: rat kidney tissue lysates, <br>
Lane 7: rat RH35 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDLR antigen affinity purified polyclonal antibody (Catalog # A00076-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDLR at approximately 130KD. The expected band size for LDLR is at 95KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-2-ldlr-primary-antibodies-ihc-testing-2.jpgAnti-LDL Receptor/LDLR Antibody Picoband® IHC analysis of LDLR using anti-LDLR antibody (A00076-2). <br>
LDLR was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LDLR Antibody (A00076-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-2-ldlr-primary-antibodies-ihc-testing-3.jpgAnti-LDL Receptor/LDLR Antibody Picoband® IHC analysis of LDLR using anti-LDLR antibody (A00076-2). <br>
LDLR was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LDLR Antibody (A00076-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-2-ldlr-primary-antibodies-fc-testing-4.pngAnti-LDL Receptor/LDLR Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-LDLR antibody (A00076-2). <br>Overlay histogram showing A431 cells stained with A00076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDLR Antibody (A00076-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-2-ldlr-primary-antibodies-fc-testing-5.pngAnti-LDL Receptor/LDLR Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-LDLR antibody (A00076-2). <br>Overlay histogram showing NRK cells stained with A00076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDLR Antibody (A00076-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lim1-lhx1-picoband-trade-antibody-a05816-1-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05816-1-lhx1-primary-antibodies-wb-testing-1.jpgAnti-LIM1/LHX1 Antibody Picoband® Western blot analysis of LHX1 using anti-LHX1 antibody (A05816-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX1 antigen affinity purified polyclonal antibody (Catalog # A05816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX1 at approximately 43KD. The expected band size for LHX1 is at 43KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05816-1-lhx1-primary-antibodies-ihc-testing-3.jpgAnti-LIM1/LHX1 Antibody Picoband®IHC analysis of LHX1 using anti-LHX1 antibody (A05816-1). <br>LHX1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHX1 Antibody (A05816-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05816-1-lhx1-primary-antibodies-ihc-testing-2.jpgAnti-LIM1/LHX1 Antibody Picoband® IHC analysis of LHX1 using anti-LHX1 antibody (A05816-1). <br>
LHX1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LHX1 Antibody (A05816-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05816-1-lhx1-primary-antibodies-fc-testing-3.pngAnti-LIM1/LHX1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-LHX1 antibody (A05816-1). <br>Overlay histogram showing PC-3 cells stained with A05816-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LHX1 Antibody (A05816-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lin28-lin28a-picoband-trade-antibody-a01966-2-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-wb-testing-1.jpgAnti-Lin28/LIN28A Antibody Picoband® Western blot analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates, <br>
Lane 4: rat testis tissue lysates, <br>
Lane 5: mouse testis tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28A antigen affinity purified polyclonal antibody (Catalog # A01966-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN28A at approximately 28KD. The expected band size for LIN28A is at 28KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-ihc-testing-2.jpgAnti-Lin28/LIN28A Antibody Picoband® IHC analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
LIN28A was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIN28A Antibody (A01966-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-ihc-testing-3.jpgAnti-Lin28/LIN28A Antibody Picoband® IHC analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
LIN28A was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIN28A Antibody (A01966-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-ihc-testing-4.jpgAnti-Lin28/LIN28A Antibody Picoband® IHC analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
LIN28A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIN28A Antibody (A01966-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-if-testing-5.jpgAnti-Lin28/LIN28A Antibody Picoband® IF analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
LIN28A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-LIN28A Antibody (A01966-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-if-testing-6.jpgAnti-Lin28/LIN28A Antibody Picoband® IF analysis of LIN28A using anti-LIN28A antibody (A01966-2). <br>
LIN28A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-LIN28A Antibody (A01966-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01966-2-lin28a-primary-antibodies-fc-testing-7_1.pngAnti-Lin28/LIN28A Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-LIN28A antibody (A01966-2). <br>Overlay histogram showing PC-3 cells stained with A01966-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN28A Antibody (A01966-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-lipc-picoband-trade-antibody-a01020-1-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01020-1-lipc-primary-antibodies-wb-testing-1_.jpgAnti-LIPC Antibody Picoband® Western blot analysis of LIPC using anti-LIPC antibody (A01020-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIPC antigen affinity purified polyclonal antibody (Catalog # A01020-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIPC at approximately 56KD. The expected band size for LIPC is at 56KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01020-1-lipc-primary-antibodies-ihc-testing-2.jpgAnti-LIPC Antibody Picoband® IHC analysis of LIPC using anti-LIPC antibody (A01020-1). <br>
LIPC was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIPC Antibody (A01020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01020-1-lipc-primary-antibodies-ihc-testing-3.jpgAnti-LIPC Antibody Picoband® IHC analysis of LIPC using anti-LIPC antibody (A01020-1). <br>
LIPC was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIPC Antibody (A01020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01020-1-lipc-primary-antibodies-if-testing-4.jpgAnti-LIPC Antibody Picoband® IF analysis of LIPC using anti-LIPC antibody (A01020-1). <br>
LIPC was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LIPC Antibody (A01020-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-lrpprc-gp130-picoband-trade-antibody-a03264-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-wb-testing-1_1.jpgAnti-LRPPRC/GP130 Antibody Picoband®Western blot analysis of LRPPRC using anti-LRPPRC antibody (A03264). <br>
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human 293T whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human Jurkat whole cell lysates, <br>
Lane 4: rat kidney tissue lysates, <br>
Lane 5: rat liver tissue lysates, <br>
Lane 6: mouse kidney tissue lysates, <br>
Lane 7: mouse liver tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRPPRC antigen affinity purified polyclonal antibody (A03264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRPPRC at approximately 158 kDa. The expected band size for LRPPRC is at 158 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-fimmu-15-1454730-g007.jpgAnti-LRPPRC/GP130 Antibody Picoband®LRPPRC expression is associated with the prognosis of patients with UCEC. (A) Kaplan-Meier survival estimates for patients with high and low LRPPRC expression. (B) Proportions of different grades and stages in high and low LRPPRC groups. (C) Representative IHC staining of LRPPRC in paracancerous and tumor UCEC samples. (D) Univariate and multivariate Cox analyses evaluating the independent prognostic value of LRPPRC in UCEC patients.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1454730/full'>39445012</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-fimmu-15-1454730-g008.jpgAnti-LRPPRC/GP130 Antibody Picoband®Knockdown of LRPPRC restrained the proliferation and invasion abilities of UCEC. (A) GO analysis displaying the normalized enrichment scores (NES) of adaptive immune response and immune cell-related pathways in UCEC patients with high LRPPRC expression. Negative NES indicates a negative correlation between the pathway and LRPPRC expression. (B) GSEA analysis showing enrichment of HALLMARK signaling pathways or processes in UCEC patients with high LRPPRC expression. (C) Western blot validation of LRPPRC knockdown in HEC-1A cells. (D) Proliferation of LRPPRC knockdown versus control cells. (E–G) Clone formation, migration, and invasion abilities of indicated UCEC cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1454730/full'>39445012</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-ihc-testing-2.jpgAnti-LRPPRC/GP130 Antibody Picoband® IHC analysis of LRPPRC using anti-LRPPRC antibody (A03264). <br>
LRPPRC was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRPPRC Antibody (A03264) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-ihc-testing-3.jpgAnti-LRPPRC/GP130 Antibody Picoband® IHC analysis of LRPPRC using anti-LRPPRC antibody (A03264). <br>
LRPPRC was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRPPRC Antibody (A03264) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-ihc-testing-4.jpgAnti-LRPPRC/GP130 Antibody Picoband® IHC analysis of LRPPRC using anti-LRPPRC antibody (A03264). <br>
LRPPRC was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LRPPRC Antibody (A03264) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-if-testing-5.jpgAnti-LRPPRC/GP130 Antibody Picoband® IF analysis of LRPPRC using anti-LRPPRC antibody (A03264). <br>
LRPPRC was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LRPPRC Antibody (A03264) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-fc-testing-6.pngAnti-LRPPRC/GP130 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-LRPPRC antibody (A03264). <br>Overlay histogram showing A431 cells stained with A03264 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRPPRC Antibody (A03264, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03264-lrpprc-primary-antibodies-fc-testing-7.pngAnti-LRPPRC/GP130 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-LRPPRC antibody (A03264). <br>Overlay histogram showing C6 cells stained with A03264 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRPPRC Antibody (A03264, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mbd4-med1-picoband-trade-antibody-a03462-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03462-mbd4-primary-antibodies-wb-testing-1.jpgAnti-MBD4/MED1 Antibody Picoband® Western blot analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human SK-OV-3 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: human hela whole cell lysates, <br>
Lane 6: human A431 whole cell lysates, <br>
Lane 7: human MDA-MB-453 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD4/MED1 antigen affinity purified polyclonal antibody (Catalog # A03462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD4/MED1 at approximately 66KD. The expected band size for MBD4/MED1 is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03462-mbd4-primary-antibodies-ihc-testing-2.jpgAnti-MBD4/MED1 Antibody Picoband® IHC analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). <br>
MBD4/MED1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MBD4/MED1 Antibody (A03462) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03462-mbd4-primary-antibodies-ihc-testing-3.jpgAnti-MBD4/MED1 Antibody Picoband® IHC analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). <br>
MBD4/MED1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MBD4/MED1 Antibody (A03462) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03462-mbd4-primary-antibodies-if-testing-4.jpgAnti-MBD4/MED1 Antibody Picoband® IF analysis of MBD4/MED1 using anti-MBD4/MED1 antibody (A03462). <br>
MBD4/MED1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MBD4/MED1 Antibody (A03462) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03462-mbd4-primary-antibodies-fcm-testing-5.pngAnti-MBD4/MED1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-MBD4/MED1 antibody (A03462). <br>Overlay histogram showing SiHa cells stained with A03462 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MBD4/MED1 Antibody (A03462, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mbnl1-picoband-trade-antibody-a02309-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-wb-testing-1.jpgAnti-MBNL1 Antibody Picoband® Western blot analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates,<br>
Lane 2: monkey COS-7 whole cell lysates,<br>
Lane 3: human 293T whole cell lysates,<br>
Lane 4: human Hela whole cell lysates,<br>
Lane 5: human MOLT-4 whole cell lysates,<br>
Lane 6: rat heart tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBNL1 antigen affinity purified polyclonal antibody (Catalog # A02309-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBNL1 at approximately 40 kDa. The expected band size for MBNL1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-2_1.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-3_1.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-4_1.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-5.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-6.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-7.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-ihc-testing-8.jpgAnti-MBNL1 Antibody Picoband® IHC analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-if-testing-9.jpgAnti-MBNL1 Antibody Picoband® IF analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02309-1-mbnl1-primary-antibodies-if-testing-10.jpgAnti-MBNL1 Antibody Picoband® IF analysis of MBNL1 using anti-MBNL1 antibody (A02309-1). <br>
MBNL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBNL1 Antibody (A02309-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-cd146-mcam-picoband-trade-antibody-a01683-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-wb-testing-1.jpgAnti-CD146/Mcam Antibody Picoband® Western blot analysis of MCAM using anti-MCAM antibody (A01683). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates, <br>
Lane 2: rat ovary tissue lysates, <br>
Lane 3: rat PC-12 whole cell lysates, <br>
Lane 4: mouse lung tissue lysates, <br>
Lane 5: mouse spleen tissue lysates, <br>
Lane 6: mouse thymus tissue lysates, <br>
Lane 7: mouse kidney tissue lysates, <br>
Lane 8: mouse ovary tissue lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAM antigen affinity purified polyclonal antibody (Catalog # A01683) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCAM at approximately 120KD. The expected band size for MCAM is at 120KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-ihc-testing-2.jpgAnti-CD146/Mcam Antibody Picoband® IHC analysis of MCAM using anti-MCAM antibody (A01683). <br>
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-ihc-testing-3.jpgAnti-CD146/Mcam Antibody Picoband® IHC analysis of MCAM using anti-MCAM antibody (A01683). <br>
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-ihc-testing-4.jpgAnti-CD146/Mcam Antibody Picoband® IHC analysis of MCAM using anti-MCAM antibody (A01683). <br>
MCAM was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCAM Antibody (A01683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-if-testing-5.jpgAnti-CD146/Mcam Antibody Picoband® IF analysis of MCAM using anti-MCAM antibody (A01683). <br>
MCAM was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MCAM Antibody (A01683) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-fc-testing-6.pngAnti-CD146/Mcam Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-MCAM antibody (A01683). <br>Overlay histogram showing C6 cells stained with A01683 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (A01683, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01683-mcam-primary-antibodies-fc-testing-7.pngAnti-CD146/Mcam Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-MCAM antibody (A01683). <br>Overlay histogram showing Neuro-2a cells stained with A01683 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAM Antibody (A01683, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mcm5-picoband-trade-antibody-a03642-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-wb-testing-1.jpgAnti-MCM5 Antibody Picoband® Western blot analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # A03642) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 100KD. The expected band size for MCM5 is at 82KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-ihc-testing-2.jpgAnti-MCM5 Antibody Picoband® IHC analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
MCM5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM5 Antibody (A03642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-ihc-testing-3.jpgAnti-MCM5 Antibody Picoband® IHC analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
MCM5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM5 Antibody (A03642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-ihc-testing-4.jpgAnti-MCM5 Antibody Picoband® IHC analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
MCM5 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM5 Antibody (A03642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-if-testing-5.jpgAnti-MCM5 Antibody Picoband® IF analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
MCM5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MCM5 Antibody (A03642) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-if-testing-6.jpgAnti-MCM5 Antibody Picoband® IF analysis of MCM5 using anti-MCM5 antibody (A03642). <br>
MCM5 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM5 Antibody (A03642) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03642-mcm5-primary-antibodies-fcm-testing-7.pngAnti-MCM5 Antibody Picoband® Flow Cytometry analysis of HEPG2 cells using anti-MCM5 antibody (A03642). <br>Overlay histogram showing HEPG2 cells stained with A03642 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM5 Antibody (A03642, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-me2-picoband-trade-antibody-a01380-1-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-wb-testing-1.jpgAnti-ME2 Antibody Picoband® Western blot analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates, <br>
Lane 2: human K562 whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human placenta tissue lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: monkey COS-7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME2 antigen affinity purified polyclonal antibody (Catalog # A01380-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME2 at approximately 65KD. The expected band size for ME2 is at 65KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-wb-testing-2_1.jpgAnti-ME2 Antibody Picoband® Western blot analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: rat kidney tissue lysates, <br>
Lane 4: mouse RAW264.7 whole cell lysates, <br>
Lane 5: mouse NIH-3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME2 antigen affinity purified polyclonal antibody (Catalog # A01380-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ME2 at approximately 65KD. The expected band size for ME2 is at 65KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-ihc-testing-3.jpgAnti-ME2 Antibody Picoband® IHC analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-ihc-testing-4.jpgAnti-ME2 Antibody Picoband® IHC analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-ihc-testing-5.jpgAnti-ME2 Antibody Picoband® IHC analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-ihc-testing-6.jpgAnti-ME2 Antibody Picoband® IHC analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-if-testing-7.jpgAnti-ME2 Antibody Picoband® IF analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01380-1-me2-primary-antibodies-if-testing-8.jpgAnti-ME2 Antibody Picoband® IF analysis of ME2 using anti-ME2 antibody (A01380-1). <br>
ME2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-ME2 Antibody (A01380-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-med4-picoband-trade-antibody-a06467-2-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-wb-testing-1_1.jpgAnti-MED4 Antibody Picoband® Western blot analysis of MED4 using anti-MED4 antibody (A06467-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human A549 whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody (Catalog # A06467-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 34KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-ihc-testing-2.jpgAnti-MED4 Antibody Picoband® IHC analysis of MED4 using anti-MED4 antibody (A06467-2). <br>
MED4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MED4 Antibody (A06467-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-ihc-testing-3.jpgAnti-MED4 Antibody Picoband® IHC analysis of MED4 using anti-MED4 antibody (A06467-2). <br>
MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MED4 Antibody (A06467-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-ihc-testing-4.jpgAnti-MED4 Antibody Picoband® IHC analysis of MED4 using anti-MED4 antibody (A06467-2). <br>
MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MED4 Antibody (A06467-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-if-testing-5.jpgAnti-MED4 Antibody Picoband® IF analysis of MED4 using anti-MED4 antibody (A06467-2). <br>
MED4 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MED4 Antibody (A06467-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06467-2-med4-primary-antibodies-fc-testing-6.pngAnti-MED4 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-MED4 antibody (A06467-2). <br>Overlay histogram showing HL-60 cells stained with A06467-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED4 Antibody (A06467-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-mesp1-picoband-trade-antibody-a07301-2-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07301-2-mesp1-primary-antibodies-wb-testing-1.jpgAnti-MESP1 Antibody Picoband® Western blot analysis of MESP1 using anti-MESP1 antibody (A07301-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: human Raji whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESP1 antigen affinity purified polyclonal antibody (Catalog # A07301-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESP1 at approximately 29KD. The expected band size for MESP1 is at 29KD.
https://www.bosterbio.com/products/primary-antibodies/anti-mnt-picoband-trade-antibody-a03357-3-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03357-3-mnt-primary-antibodies-wb-testing-1.jpgAnti-MNT Antibody Picoband® Western blot analysis of MNT using anti-MNT antibody (A03357-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human Raji whole cell lysates, <br>
Lane 3: human A549 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human K562 whole cell lysates, <br>
Lane 6: human PC-3 whole cell lysates, <br>
Lane 7: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MNT antigen affinity purified polyclonal antibody (Catalog # A03357-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MNT at approximately 62KD. The expected band size for MNT is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03357-3-mnt-primary-antibodies-ihc-testing-2.jpgAnti-MNT Antibody Picoband® IHC analysis of MNT using anti-MNT antibody (A03357-3). <br>
MNT was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MNT Antibody (A03357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03357-3-mnt-primary-antibodies-ihc-testing-3.jpgAnti-MNT Antibody Picoband® IHC analysis of MNT using anti-MNT antibody (A03357-3). <br>
MNT was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MNT Antibody (A03357-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03357-3-mnt-primary-antibodies-if-testing-4.jpgAnti-MNT Antibody Picoband® IF analysis of MNT using anti-MNT antibody (A03357-3). <br>
MNT was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MNT Antibody (A03357-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03357-3-mnt-primary-antibodies-fc-testing-5.pngAnti-MNT Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-MNT antibody (A03357-3). <br>
Overlay histogram showing A431 cells stained with A03357-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MNT Antibody (A03357-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-musashi-1-msi1-picoband-trade-antibody-a05052-2-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05052-2-msi1-primary-antibodies-wb-testing-1.jpgAnti-Musashi 1/Msi1 Antibody Picoband® Western blot analysis of MSI1 using anti-MSI1 antibody (A05052-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human T-47D whole cell lysates, <br>
Lane 3: human COLO-320 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSI1 antigen affinity purified polyclonal antibody (Catalog # A05052-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSI1 at approximately 39KD. The expected band size for MSI1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05052-2-msi1-primary-antibodies-fc-testing-2.pngAnti-Musashi 1/Msi1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-MSI1 antibody (A05052-2). <br>
Overlay histogram showing A549 cells stained with A05052-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSI1 Antibody (A05052-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-nadh2-mtnd2-picoband-trade-antibody-a32839-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32839-mtnd2-primary-antibodies-wb-testing-1_.jpgAnti-NADH2/Mtnd2 Antibody Picoband® Western blot analysis of Mtnd2 using anti-Mtnd2 antibody (A32839). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat thymus tissue lysates, <br>
Lane 2: rat spleen tissue lysates, <br>
Lane 3: mouse thymus tissue lysates, <br>
Lane 4: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mtnd2 antigen affinity purified polyclonal antibody (Catalog # A32839) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mtnd2 at approximately 39KD. The expected band size for Mtnd2 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32839-mtnd2-primary-antibodies-fc-testing-2.pngAnti-NADH2/Mtnd2 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Mtnd2 antibody (A32839). <br>Overlay histogram showing ANA-1 cells stained with A32839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mtnd2 Antibody (A32839, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32839-mtnd2-primary-antibodies-fc-testing-3.pngAnti-NADH2/Mtnd2 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-Mtnd2 antibody (A32839). <br>Overlay histogram showing NRK cells stained with A32839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mtnd2 Antibody (A32839, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nadh2-mtnd2-picoband-trade-antibody-a32839-1-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32839-1-mtnd2-primary-antibodies-fcm-testing-2.jpgAnti-NADH2/Mtnd2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-NADH2/Mtnd2 antibody (A32839-1). <br>Overlay histogram showing RH35 cells stained with A32839-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NADH2/Mtnd2 Antibody (A32839-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32839-1-mtnd2-primary-antibodies-wb-testing-1_1.jpgAnti-NADH2/Mtnd2 Antibody Picoband® Western blot analysis of NADH2/Mtnd2 using anti-NADH2/Mtnd2 antibody (A32839-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: mouse brain tissue lysates. <br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADH2/Mtnd2 antigen affinity purified polyclonal antibody (Catalog # A32839-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADH2/Mtnd2 at approximately 39 kDa. The expected band size for NADH2/Mtnd2 is at 39 kDa.
https://www.bosterbio.com/products/primary-antibodies/anti-nectin-2-nectin2-picoband-trade-antibody-a08081-2-boster.html2026-03-24T05:26:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-nectin2-primary-antibodies-wb-testing-1.jpgAnti-Nectin 2/NECTIN2 Antibody Picoband® Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 2/NECTIN2 antigen affinity purified polyclonal antibody (Catalog # A08081-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 2/NECTIN2 at approximately 70-80KD. The expected band size for Nectin 2/NECTIN2 is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-41419_2024_6571_fig6_html.pngAnti-Nectin 2/NECTIN2 Antibody Picoband®The comprehensive immunosuppressive mechanism in imatinib resistant GIST. a Cell communication analysis on TIGIT-NECTIN2 and BTLA-TNFRSF14 pair between different cell types in imatinib resistant and sensitive patients respectively. Expression of NECTIN2 ( b ), BTLA ( c ) and TNFRSF14 ( d ) in each cell type in imatinib resistant and sensitive patients respectively. e IHC analysis of NECTIN2, BTLA and TNFRSF14 between imatinib resistant (upper) and sensitive (bottom) patients. f Schematic diagram of the unique tumor-immune microenvironment of imatinib-resistance in advanced GIST. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-06571-3'>38443340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-nectin2-primary-antibodies-ihc-testing-2.jpgAnti-Nectin 2/NECTIN2 Antibody Picoband® IHC analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2). <br>
Nectin 2/NECTIN2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nectin 2/NECTIN2 Antibody (A08081-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-nectin2-primary-antibodies-fcm-testing-3.pngAnti-Nectin 2/NECTIN2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Nectin 2/NECTIN2 antibody (A08081-2).<br>Overlay histogram showing HepG2 cells stained with A08081-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nectin 2/NECTIN2 Antibody (A08081-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-nectin2-primary-antibodies-ihc-testing-4.jpgAnti-Nectin 2/NECTIN2 Antibody Picoband® IHC analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2).<br>
Nectin 2/NECTIN2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nectin 2/NECTIN2 Antibody (A08081-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08081-2-nectin2-primary-antibodies-if-testing-5.jpgAnti-Nectin 2/NECTIN2 Antibody Picoband® IF analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2). <br>
Nectin 2/NECTIN2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Nectin 2/NECTIN2 Antibody (A08081-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-nectin-3-nectin3-picoband-trade-antibody-a09633-3-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09633-3-nectin3-primary-antibodies-wb-testing-1_1.jpgAnti-Nectin 3/NECTIN3 Antibody Picoband® Western blot analysis of Nectin 3/NECTIN3 using anti-Nectin 3/NECTIN3 antibody (A09633-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U20S whole cell lysates. <br>
Lane 2: human HEK293 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 3/NECTIN3 antigen affinity purified polyclonal antibody (Catalog # A09633-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 3/NECTIN3 at approximately 80KD. The expected band size for Nectin 3/NECTIN3 is at 61KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09633-3-nectin3-primary-antibodies-ihc-testing-2.jpgAnti-Nectin 3/NECTIN3 Antibody Picoband® IHC analysis of Nectin 3/NECTIN3 using anti-Nectin 3/NECTIN3 antibody (A09633-3). <br>
Nectin 3/NECTIN3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nectin 3/NECTIN3 Antibody (A09633-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09633-3-nectin3-primary-antibodies-fcm-testing-3.pngAnti-Nectin 3/NECTIN3 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Nectin 3/NECTIN3 antibody (A09633-3).<br>Overlay histogram showing A431 cells stained with A09633-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nectin 3/NECTIN3 Antibody (A09633-3,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nfic-ctf-picoband-trade-antibody-a04154-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-wb-testing-1__1.jpgAnti-NFIC/CTF Antibody Picoband® Western blot analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human U2OS whole cell lysates, <br>
Lane 3: human T-47D whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human HepG2 whole cell lysates, <br>
Lane 6: human A431 whole cell lysates, <br>
Lane 7: human Caco-2 whole cell lysates, <br>
Lane 8: rat skeletal muscle tissue lysates, <br>
Lane 9: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIC antigen affinity purified polyclonal antibody (Catalog # A04154-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for NFIC at approximately 55-65, 70KD. The expected band size for NFIC is at 56KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-ihc-testing-2.jpgAnti-NFIC/CTF Antibody Picoband® IHC analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
NFIC was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIC Antibody (A04154-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-ihc-testing-3.jpgAnti-NFIC/CTF Antibody Picoband® IHC analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
NFIC was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIC Antibody (A04154-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-ihc-testing-4.jpgAnti-NFIC/CTF Antibody Picoband® IHC analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
NFIC was detected in paraffin-embedded section of rat pancreas muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NFIC Antibody (A04154-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-if-testing-5.jpgAnti-NFIC/CTF Antibody Picoband® IF analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
NFIC was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-NFIC Antibody (A04154-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-if-testing-6.jpgAnti-NFIC/CTF Antibody Picoband® IF analysis of NFIC using anti-NFIC antibody (A04154-2). <br>
NFIC was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-NFIC Antibody (A04154-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04154-2-nfic-primary-antibodies-fc-testing-7.pngAnti-NFIC/CTF Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-NFIC antibody (A04154-2). <br>Overlay histogram showing A549 cells stained with A04154-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIC Antibody (A04154-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-p75-ngf-receptor-ngfr-picoband-trade-antibody-a01187-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01187-ngfr-primary-antibodies-wb-testing-1.jpgAnti-p75 NGF Receptor/NGFR Antibody Picoband® Western blot analysis of p75 NGF Receptor/NGFR using anti-p75 NGF Receptor/NGFR antibody (A01187). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: human Sw620 whole cell lysates, <br>
Lane 3: human Caco-2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p75 NGF Receptor/NGFR antigen affinity purified polyclonal antibody (Catalog # A01187) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p75 NGF Receptor/NGFR at approximately 45KD,75KD. The expected band size for p75 NGF Receptor/NGFR is at 45KD,75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01187-ngfr-primary-antibodies-if-testing-2.jpgAnti-p75 NGF Receptor/NGFR Antibody Picoband® IF analysis of p75 NGF Receptor/NGFR using anti-p75 NGF Receptor/NGFR antibody (A01187). <br>
p75 NGF Receptor/NGFR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-p75 NGF Receptor/NGFR Antibody (A01187) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01187-ngfr-primary-antibodies-fcm-testing-3.pngAnti-p75 NGF Receptor/NGFR Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-p75 NGF Receptor/NGFR antibody (A01187). <br>Overlay histogram showing A431 cells stained with A01187 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p75 NGF Receptor/NGFR Antibody (A01187, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-nova2-picoband-trade-antibody-a10622-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-wb-testing-1.jpgAnti-NOVA2 Antibody Picoband® Western blot analysis of NOVA2 using anti-NOVA2 antibody (A10622-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human U2OS whole cell lysates, <br>
Lane 2: human HL-60 whole cell lysates, <br>
Lane 3: human THP-1 whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: rat spleen tissue lysates, <br>
Lane 6: rat lung tissue lysates, <br>
Lane 7: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOVA2 antigen affinity purified polyclonal antibody (Catalog # A10622-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOVA2 at approximately 72KD. The expected band size for NOVA2 is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-ihc-testing-2.jpgAnti-NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (A10622-2). <br>
NOVA2 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOVA2 Antibody (A10622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-ihc-testing-3.jpgAnti-NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (A10622-2). <br>
NOVA2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOVA2 Antibody (A10622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-ihc-testing-4.jpgAnti-NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (A10622-2). <br>
NOVA2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOVA2 Antibody (A10622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-ihc-testing-5.jpgAnti-NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (A10622-2). <br>
NOVA2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOVA2 Antibody (A10622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-fc-testing-6.pngAnti-NOVA2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-NOVA2 antibody (A10622-2). <br>Overlay histogram showing A549 cells stained with A10622-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOVA2 Antibody (A10622-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10622-2-nova2-primary-antibodies-fc-testing-7_1.pngAnti-NOVA2 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-NOVA2 antibody (A10622-2). <br>Overlay histogram showing HEPA1-6 cells stained with A10622-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOVA2 Antibody (A10622-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-oas3-picoband-trade-antibody-a05032-1-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05032-1-oas3-primary-antibodies-wb-testing-1.jpgAnti-OAS3 Antibody Picoband® Western blot analysis of OAS3 using anti-OAS3 antibody (A05032-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human PC-3 whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human A549 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAS3 antigen affinity purified polyclonal antibody (Catalog # A05032-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAS3 at approximately 123KD. The expected band size for OAS3 is at 123KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05032-1-oas3-primary-antibodies-ihc-testing-2.jpgAnti-OAS3 Antibody Picoband® IF analysis of OAS3 using anti-OAS3 antibody (A05032-1). <br>
OAS3 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-OAS3 Antibody (A05032-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05032-1-oas3-primary-antibodies-fc-testing-3.pngAnti-OAS3 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-OAS3 antibody (A05032-1). <br>Overlay histogram showing SiHa cells stained with A05032-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OAS3 Antibody (A05032-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pck2-picoband-trade-antibody-a04772-1-boster.html2026-03-26T05:20:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-wb-testing-1_1.jpgAnti-PCK2 Antibody Picoband®Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: human T47D whole cell lysates,<br>
Lane 2: human U20S whole cell lysates,<br>
Lane 3: human A431 whole cell lysates,<br>
Lane 4: human K562 whole cell lysates,<br>
Lane 5: monkey COS-7 whole cell lysates,<br>
Lane 6: monkey lung tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (A04772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71 kDa. The expected band size for PCK2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-wb-testing-2.jpgAnti-PCK2 Antibody Picoband®Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>
Lane 1: rat kidney tissue lysates,<br>
Lane 2: rat liver tissue lysates,<br>
Lane 3: mouse lung tissue lysates,<br>
Lane 4: mouse spleen tissue lysates,<br>
Lane 5: mouse kidney tissue lysates,<br>
Lane 6: mouse liver tissue lysates.<br>
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (A04772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71 kDa. The expected band size for PCK2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-2_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-3_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-4_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-5_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-6_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-7_1.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-8.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-ihc-testing-9.jpgAnti-PCK2 Antibody Picoband®IHC analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>PCK2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-if-testing-1.jpgAnti-PCK2 Antibody Picoband®IF analysis of PCK2 using anti-PCK2 antibody (A04772-1). <br>
PCK2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PCK2 Antibody (A04772-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04772-1-pck2-primary-antibodies-fcm-testing-1.jpgAnti-PCK2 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PCK2 antibody (A04772-1). <br>
Overlay histogram showing MCF-7 cells stained with A04772-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCK2 Antibody (A04772-1, 1 μg/1x10<sup>6</sup> cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-phex-picoband-trade-antibody-a02078-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02078-phex-primary-antibodies-wb-testing-1.jpgAnti-PHEX Antibody Picoband® Western blot analysis of PHEX using anti-PHEX antibody (A02078). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat ovary tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: mouse ovary tissue lysates, <br>
Lane 4: mouse kidney tissue lysates, <br>
Lane 5: human HEK293 whole cell lysates, <br>
Lane 6: monkey kidney tissue lysates, <br>
Lane 7: rat NRK whole cell lysates, <br>
Lane 8: monkey COS-7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHEX antigen affinity purified polyclonal antibody (Catalog # A02078) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHEX at approximately 86KD. The expected band size for PHEX is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02078-phex-primary-antibodies-ihc-testing-2.jpgAnti-PHEX Antibody Picoband® IHC analysis of PHEX using anti-PHEX antibody (A02078). <br>
PHEX was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHEX Antibody (A02078) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02078-phex-primary-antibodies-ihc-testing-3.jpgAnti-PHEX Antibody Picoband® IHC analysis of PHEX using anti-PHEX antibody (A02078). <br>
PHEX was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PHEX Antibody (A02078) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02078-phex-primary-antibodies-fc-testing-4.pngAnti-PHEX Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-PHEX antibody (A02078). <br>Overlay histogram showing U87 cells stained with A02078 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PHEX Antibody (A02078, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pias1-picoband-trade-antibody-a01707-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01707-pias1-primary-antibodies-wb-testing-1.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® Western blot analysis of PIAS1 using anti-PIAS1 antibody (A01707). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human Jurkat whole cell lysates, <br>
Lane 3: human HEK293 whole cell lysates, <br>
Lane 4: human Raji whole cell lysates, <br>
Lane 5: human K562 whole cell lysates, <br>
Lane 6: human Hela whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 antigen affinity purified polyclonal antibody (Catalog # A01707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS1 at approximately 72KD. The expected band size for PIAS1 is at 72KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01707-pias1-primary-antibodies-if-testing-2.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® IF analysis of PIAS1 using anti-PIAS1 antibody (A01707). <br>
PIAS1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PIAS1 Antibody (A01707) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01707-pias1-primary-antibodies-fc-testing-3_1.pngAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PIAS1 antibody (A01707). <br>Overlay histogram showing PC-3 cells stained with A01707 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIAS1 Antibody (A01707, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-pias2-picoband-trade-antibody-a04130-3-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04130-3-pias2-primary-antibodies-wb-testing-1_1.jpgAnti-PIAS2 Antibody Picoband® Western blot analysis of PIAS2 using anti-PIAS2 antibody (A04130-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human HepG2 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human HL-60 whole cell lysates, <br>
Lane 6: human Raji whole cell lysates, <br>
Lane 7: human A431 whole cell lysates, <br>
Lane 8: rat brain tissue lysates, <br>
Lane 9: rat C6 whole cell lysates, <br>
Lane 10: mouse brain tissue lysates, <br>
Lane 11: mouse testis tissue lysates, <br>
Lane 12: mouse Neuro-2a whole cell lysates, <br>
Lane 13: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS2 antigen affinity purified polyclonal antibody (Catalog # A04130-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS2 at approximately 68KD. The expected band size for PIAS2 is at 68KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04130-3-pias2-primary-antibodies-fc-testing-2.pngAnti-PIAS2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PIAS2 antibody (A04130-3). <br>Overlay histogram showing U937 cells stained with A04130-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIAS2 Antibody (A04130-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rna-polymerase-ii-polr2a-picoband-trade-antibody-a01029-1-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-wb-testing-1.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® Western blot analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: monkey COS-7 whole cell lysates, <br>
Lane 3: human A431 whole cell lysates, <br>
Lane 4: human PC-3 whole cell lysates, <br>
Lane 5: human Caco-2 whole cell lysates, <br>
Lane 6: human SW620 whole cell lysates, <br>
Lane 7: human HEK293 whole cell lysates, <br>
Lane 8: rat PC-12 whole cell lysates, <br>
Lane 9: mouse testis tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A antigen affinity purified polyclonal antibody (Catalog # A01029-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2A at approximately 220KD. The expected band size for POLR2A is at 220KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-ihc-testing-2.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® IHC analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
POLR2A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-ihc-testing-3.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® IHC analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
POLR2A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-ihc-testing-4.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® IHC analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
POLR2A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-ihc-testing-5.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® IHC analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
POLR2A was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POLR2A Antibody (A01029-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-if-testing-6_.jpgAnti-RNA polymerase II/POLR2A Antibody Picoband® IF analysis of POLR2A using anti-POLR2A antibody (A01029-1). <br>
POLR2A was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-POLR2A Antibody (A01029-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01029-1-polr2a-primary-antibodies-fc-testing-7_1.pngAnti-RNA polymerase II/POLR2A Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-POLR2A antibody (A01029-1). <br>Overlay histogram showing HL-60 cells stained with A01029-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLR2A Antibody (A01029-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-prdm5-picoband-trade-antibody-a06736-1-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-wb-testing-1.jpgAnti-PRDM5 Antibody Picoband® Western blot analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HL-60 whole cell lysates, <br>
Lane 2: rat ovary tissue lysates, <br>
Lane 3: mouse ovary tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM5 antigen affinity purified polyclonal antibody (Catalog # A06736-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM5 at approximately 75KD. The expected band size for PRDM5 is at 75KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-2.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-3.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-4.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-5.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-6.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-7.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of rat Intestinal lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-ihc-testing-8.jpgAnti-PRDM5 Antibody Picoband® IHC analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-if-testing-9.jpgAnti-PRDM5 Antibody Picoband® IF analysis of PRDM5 using anti-PRDM5 antibody (A06736-1). <br>
PRDM5 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PRDM5 Antibody (A06736-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06736-1-prdm5-primary-antibodies-fc-testing-10.pngAnti-PRDM5 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-PRDM5 antibody (A06736-1). <br>Overlay histogram showing CACO-2 cells stained with A06736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM5 Antibody (A06736-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ptf1a-picoband-trade-antibody-a03891-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03891-2-ptf1a-primary-antibodies-wb-testing-1.jpgAnti-PTF1A Antibody Picoband® Western blot analysis of PTF1A using anti-PTF1A antibody (A03891-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat pancreas tissue lysates, <br>
Lane 2: mouse pancreas tissue lysates, <br>
Lane 3: human PANC-1 whole cell lysates, <br>
Lane 4: human PANC-1 whole cell lysates, <br>
Lane 5: human U2OS whole cell lysates, <br>
Lane 6: mouse SP2-0 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTF1A antigen affinity purified polyclonal antibody (Catalog # A03891-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTF1A at approximately 48KD. The expected band size for PTF1A is at 35KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03891-2-ptf1a-primary-antibodies-if-testing-2.jpgAnti-PTF1A Antibody Picoband® IF analysis of PTF1A using anti-PTF1A antibody (A03891-2). <br>
PTF1A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PTF1A Antibody (A03891-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03891-2-ptf1a-primary-antibodies-fc-testing-3.pngAnti-PTF1A Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PTF1A antibody (A03891-2). <br>Overlay histogram showing A431 cells stained with A03891-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTF1A Antibody (A03891-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rbpms-picoband-trade-antibody-a07130-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-wb-testing-1.jpgAnti-RBPMS Antibody Picoband® Western blot analysis of RBPMS using anti-RBPMS antibody (A07130-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Raji whole cell lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse NIH/3T3 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPMS antigen affinity purified polyclonal antibody (Catalog # A07130-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBPMS at approximately 22KD. The expected band size for RBPMS is at 22KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-ihc-testing-2.jpgAnti-RBPMS Antibody Picoband® IHC analysis of RBPMS using anti-RBPMS antibody (A07130-2). <br>
RBPMS was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBPMS Antibody (A07130-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-ihc-testing-3.jpgAnti-RBPMS Antibody Picoband® IHC analysis of RBPMS using anti-RBPMS antibody (A07130-2). <br>
RBPMS was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBPMS Antibody (A07130-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-ihc-testing-4.jpgAnti-RBPMS Antibody Picoband® IHC analysis of RBPMS using anti-RBPMS antibody (A07130-2). <br>
RBPMS was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBPMS Antibody (A07130-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-ihc-testing-5.jpgAnti-RBPMS Antibody Picoband® IHC analysis of RBPMS using anti-RBPMS antibody (A07130-2). <br>
RBPMS was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBPMS Antibody (A07130-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07130-2-rbpms-primary-antibodies-fcm-testing-6.pngAnti-RBPMS Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-RBPMS antibody (A07130-2). <br>Overlay histogram showing CACO-2 cells stained with A07130-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBPMS Antibody (A07130-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rec8-picoband-trade-antibody-a04915-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04915-2-rec8-primary-antibodies-wb-testing-1_.jpgAnti-REC8 Antibody Picoband® Western blot analysis of REC8 using anti-REC8 antibody (A04915-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Jurkat whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REC8 antigen affinity purified polyclonal antibody (Catalog # A04915-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REC8 at approximately 63KD. The expected band size for REC8 is at 63KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04915-2-rec8-primary-antibodies-ihc-testing-2.jpgAnti-REC8 Antibody Picoband® IHC analysis of REC8 using anti-REC8 antibody (A04915-2). <br>
REC8 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-REC8 Antibody (A04915-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04915-2-rec8-primary-antibodies-ihc-testing-3.jpgAnti-REC8 Antibody Picoband® IHC analysis of REC8 using anti-REC8 antibody (A04915-2). <br>
REC8 was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-REC8 Antibody (A04915-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04915-2-rec8-primary-antibodies-ihc-testing-4.jpgAnti-REC8 Antibody Picoband® IHC analysis of REC8 using anti-REC8 antibody (A04915-2). <br>
REC8 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-REC8 Antibody (A04915-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04915-2-rec8-primary-antibodies-ihc-testing-5.pngAnti-REC8 Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-REC8 antibody (A04915-2). <br>
Overlay histogram showing HeLa cells stained with A04915-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REC8 Antibody (A04915-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-psgl-1-selplg-picoband-trade-antibody-a03674-3-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-3-selplg-primary-antibodies-wb-testing-1.jpgAnti-PSGL-1/Selplg Antibody Picoband® Western blot analysis of SELPLG using anti-SELPLG antibody (A03674-3). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELPLG antigen affinity purified polyclonal antibody (Catalog # A03674-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELPLG at approximately 110-120KD. The expected band size for SELPLG is at 110-120KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-3-selplg-primary-antibodies-ihc-testing-2.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-3). <br>
SELPLG was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-3-selplg-primary-antibodies-ihc-testing-3.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-3). <br>
SELPLG was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-3-selplg-primary-antibodies-fc-testing-4.pngAnti-PSGL-1/Selplg Antibody Picoband® Flow Cytometry analysis of mouse spleen cells using anti-SELPLG antibody (A03674-3). <br>
Overlay histogram showing mouse spleen cells stained with A03674-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SELPLG Antibody (A03674-3, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
https://www.bosterbio.com/products/primary-antibodies/anti-psgl-1-selplg-picoband-trade-antibody-a03674-4-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-wb-testing-1.jpgAnti-PSGL-1/Selplg Antibody Picoband® Western blot analysis of SELPLG using anti-SELPLG antibody (A03674-4). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat spleen tissue lysates, <br>
Lane 2: rat thymus tissue lysates, <br>
Lane 3: mouse thymus tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELPLG antigen affinity purified polyclonal antibody (Catalog # A03674-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELPLG at approximately 110-120KD. The expected band size for SELPLG is at 42KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-ihc-testing-2.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-4). <br>
SELPLG was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-ihc-testing-3.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-4). <br>
SELPLG was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-ihc-testing-4.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-4). <br>
SELPLG was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-ihc-testing-5.jpgAnti-PSGL-1/Selplg Antibody Picoband® IHC analysis of SELPLG using anti-SELPLG antibody (A03674-4). <br>
SELPLG was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SELPLG Antibody (A03674-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03674-4-selplg-primary-antibodies-fc-testing-6.pngAnti-PSGL-1/Selplg Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-SELPLG antibody (A03674-4). <br>Overlay histogram showing NRK cells stained with A03674-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SELPLG Antibody (A03674-4, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-kallistatin-pi-4-serpina4-picoband-trade-antibody-a08426-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08426-serpina4-primary-antibodies-wb-testing-1.jpgAnti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® Western blot analysis of SERPINA4 using anti-SERPINA4 antibody (A08426). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat liver tissue lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: monkey liver tissue lysates, <br>
Lane 4: monkey liver tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA4 antigen affinity purified polyclonal antibody (Catalog # A08426) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA4 at approximately 49KD. The expected band size for SERPINA4 is at 49KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08426-serpina4-primary-antibodies-ihc-testing-2.jpgAnti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® IHC analysis of SERPINA4 using anti-SERPINA4 antibody (A08426). <br>
SERPINA4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SERPINA4 Antibody (A08426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/products/primary-antibodies/anti-hset1-set1-setd1a-picoband-trade-antibody-a03736-1-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03736-1-setd1a-primary-antibodies-wb-testing-1.jpgAnti-SET1/SETD1A Antibody Picoband® Western blot analysis of SET1/SETD1A using anti-SET1/SETD1A antibody (A03736-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human SW620 whole cell lysates, <br>
Lane 2: human U87 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SET1/SETD1A antigen affinity purified polyclonal antibody (Catalog # A03736-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SET1/SETD1A at approximately 186KD. The expected band size for SET1/SETD1A is at 186KD.
https://www.bosterbio.com/products/primary-antibodies/anti-glut10-slc2a10-picoband-trade-antibody-a06461-2-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06461-2-slc2a10-primary-antibodies-wb-testing-1.jpgAnti-GLUT10/SLC2A10 Antibody Picoband® Western blot analysis of GLUT10/SLC2A10 using anti-GLUT10/SLC2A10 antibody (A06461-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT10/SLC2A10 antigen affinity purified polyclonal antibody (Catalog # A06461-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT10/SLC2A10 at approximately 67KD. The expected band size for GLUT10/SLC2A10 is at 54KD.
https://www.bosterbio.com/products/primary-antibodies/anti-vinexin-sorbs3-picoband-trade-antibody-a05794-boster.html2026-03-24T05:26:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-wb-testing-1.jpgAnti-Vinexin/SORBS3 Antibody Picoband® Western blot analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human THP-1 whole cell lysates, <br>
Lane 2: rat liver tissue lysates, <br>
Lane 3: mouse brain tissue lysates, <br>
Lane 4: mouse HEPA1-6 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS3 antigen affinity purified polyclonal antibody (Catalog # A05794) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS3 at approximately 87KD. The expected band size for SORBS3 is at 87KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-2.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IHC analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-3.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IHC analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-4.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IHC analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-5.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IHC analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in paraffin-embedded section of rat intestinal lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-6.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IHC analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-ihc-testing-7_.jpgAnti-Vinexin/SORBS3 Antibody Picoband® IF analysis of SORBS3 using anti-SORBS3 antibody (A05794). <br>
SORBS3 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SORBS3 Antibody (A05794) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05794-sorbs3-primary-antibodies-fc-testing-8.pngAnti-Vinexin/SORBS3 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SORBS3 antibody (A05794). <br>Overlay histogram showing A431 cells stained with A05794 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SORBS3 Antibody (A05794, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-spry-2-spry2-picoband-trade-antibody-a02089-2-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02089-2-spry2-primary-antibodies-wb-testing-1.jpgAnti-Spry-2/SPRY2 Antibody Picoband® Western blot analysis of Spry-2/SPRY2 using anti-Spry-2/SPRY2 antibody (A02089-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates, <br>
Lane 2: rat brain tissue lysates,<br>
Lane 3: mouse brain tissue lysates,<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spry-2/SPRY2 antigen affinity purified polyclonal antibody (Catalog # A02089-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spry-2/SPRY2 at approximately 35KD. The expected band size for Spry-2/SPRY2 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02089-2-spry2-primary-antibodies-fcm-testing-2.jpgAnti-Spry-2/SPRY2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Spry-2/SPRY2 antibody (A02089-2).<br>Overlay histogram showing U87 cells stained with A02089-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spry-2/SPRY2 Antibody (A02089-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-sprouty-4-spry-4-spry4-picoband-trade-antibody-a04343-2-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-spry4-primary-antibodies-wb-testing-1.jpgAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband® Western blot analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human PC-3 whole cell lysates, <br>
Lane 2: human HepG2 whole cell lysates,<br>
Lane 3: human K562 whole cell lysates,<br>
Lane 4: human A375 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sprouty 4/Spry-4/SPRY4 antigen affinity purified polyclonal antibody (Catalog # A04343-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sprouty 4/Spry-4/SPRY4 at approximately 35KD. The expected band size for Sprouty 4/Spry-4/SPRY4 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-41467_2024_51488_fig5_html.pngAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband®Mdm2 knockdown decreases cell migration, attachment to the ECM, and FA formation while induces expression of Sprouty4 in naturally p53 null lung adenocarcinoma cell line. H1299 cells were transfected with siRNAs against Mdm2 and siCtrl. A Protein levels of Mdm2, MdmX, and p53 after. β-actin was used as a loading control, n = 4 samples. B Cell migration assay. Representative images (top) and quantification (bottom) of wound scratch migration assay, n = 3 groups. C Quantification and representative micrographs showing attachment to ECM component, collagen I, n = 3 samples. D Immunofluorescence showing FA foci by vinculin staining (red), cell surface was outlined by phalloidin staining (green), and nuclei (blue) detected by DAPI staining of DNA, n = 3 groups. Representative images are shown on the left, and the quantification of FA parameters is shown on the right. E Protein levels of Spry4 as well as Mdm2, MdmX, and p53 after Mdm2 silencing using siRNAs. β-actin was used as a loading control, n = 4 samples. F mRNA levels of Spry4 after Mdm2 silencing using siRNAs, n = 3 samples. RPL32 was used as housekeeping. Graphs shown represent the mean ± SD of independent experimental replicates. More details about the statistical tests used can be found in the Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-51488-2'>39164253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-41467_2024_51488_fig4_html.pngAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband®The Mdm2/MdmX complex regulates the expression and subcellular localization of Sprouty4. HT1080 p53KO cells were transfected with siRNAs against Mdm2 or siCtrl for 24 h; or treated with 7 µM MEL23 or DMSO (vehicle) for 24 h. A Volcano plots show all proteins identified by mass spectrometry. Colored dots represent significantly differentially expressed proteins that were downregulated (blue dots) and upregulated (red dots) in each condition shown at the left of the plot. Gray dots represent non-significant changes. MEL23-treated cells were compared to DMSO-treated cells. Cells transfected with siMdm2#1 or #2 were compared to siCtrl-transfected cells, n = 3 samples. Black arrows point to the location of Spry4 in each Volcano plot. B Spry4 expression in HT1080 p53KO cells in response to Mdm2 knockdown or treatment with MEL23 for 24 h by immunoblotting. β-actin and α-tubulin were used as loading control for immunoblot, n = 6 samples. C Co-immunoprecipitation of Mdm2 and Spry4 in the presence or absence of MG132. β-actin was used as a loading control. Mdm2 was pulled down by using either a mix of antibodies against Mdm2 (4B11, 3G5, and 2A9) that recognize different domains within the protein, this condition was called “mix”, or by using a single monoclonal antibody D1V2Z, this condition was called “DIV”, n = 3 samples. D Quantification of Spry4, Mdm2, and MdmX protein levels after treatment with MG132 (or vehicle, DMSO) for 4 h, n = 3 samples. E Spry4 mRNA levels in response to Mdm2 knockdown ( n = 5 samples) or treatment with MEL23 for 24 h ( n = 4 samples). RPL32 was used as housekeeping. F Localization of Spry4 in HT1080 p53KO cells in response to Mdm2 knockdown. Immunofluorescence showed Spry4 staining (green), the cell surface was outlined by phalloidin staining (orange), and nuclei (blue) were detected by DAPI staining of DNA, n = 3 groups. Graphs shown in ( D , E ) represent the mean ± SD of independent experimental replicates. More details about the statistical tests used can be found in the Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-51488-2'>39164253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-41467_2024_51488_fig6_html.pngAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband®Knockdown of Sprouty4 rescues changes in cell migration, FA formation, and metastasis resulting from loss of Mdm2. A – D HT1080 p53KO cells were transfected with siRNA against Mdm2 alone or both siRNA against Mdm2 and a pool of Spry4 siRNAs. A Protein levels of Mdm2, MdmX, and Spry4 after transfection with indicated siRNAs, n = 3 samples. β-actin was used as a loading control. B Quantification of wound scratch migration assay in cells treated with the indicated siRNAs as in Fig. , n = 3 samples. C Quantification of cell area after attachment to collagen-coated coverslips as in Fig. , n = 3 samples. D Immunofluorescence showing FA foci by vinculin staining (red), cell surface was outlined by phalloidin staining (green), and nuclei (blue) as detected by DAPI staining, n = 3 groups. Representative images are shown on the left, and the quantification of FA parameters is shown on the right. In ( C , D ) the graphs shown represent mean ± SD of independent experimental replicates and in each replicate all parameters were quantified in at least 20 events/condition for total of at least 60 events/condition. E , F H1299 cells were transfected with siRNA against Mdm2 alone or both siRNA against Mdm2 and a pool of Spry4 siRNAs. E Protein levels of Mdm2, MdmX, and Spry4 after transfection with indicated siRNAs. β-actin was used as a loading control, n = 3 samples. F Quantification of wound scratch migration assay comparing migration into wound scratches in cells treated with the indicated siRNAs as in Fig. , n = 3 samples. G , H HT1080 p53KO cell lines were established stably expressing a pool of shRNAs against Mdm2 alone or Mdm2 and Spry4 together. G Protein levels of Mdm2, MdmX, and Spry4 in stable cell lines. β-actin was used as a loading control, n = 3 samples. H Analysis of metastatic burden in vivo using tail-vein injection model as in Fig. H, . Representative images above and quantification below of metastatic foci in the lungs after 8 weeks of injection, n = 7 mice/group. The graphs shown represent the mean ± SD of independent experimental replicates. More details about the statistical tests used can be found in the Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-51488-2'>39164253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-41467_2024_51488_fig7_html.pngAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband®Sprouty4 acts through a non-canonical pathway, by regulating RhoA levels, to induce the effects of Mdm2 knockdown in cell migration. A , B Effect of Spry4 modulation in the phosphorylation of ERKs in HT1080 p53KO cells. Protein levels of phospho-ERK and total ERK levels ( A ) after Spry4 silencing using a pool of siRNAs and ( B ) in response to silencing of Mdm2 alone or Mdm2 and Spry4 together. C , D RhoA modulation in HT1080 p53KO cells transfected with siRNAs against Mdm2 or treated with MEL23. C Protein levels and ( D ) mRNA levels of RhoA after transfection with indicated siRNAs ( n = 3 samples) or treatment with MEL23 ( n = 3 samples) for 24 h. β-actin was used as a loading control for immunoblots. RPL32 was used as a housekeeping control for qPCR. E Immunoprecipitation of Mdm2 in the presence of MG132. Lysates were probed for the presence of RhoA, and MdmX was used as a positive control. Mdm2 was pulled down by using a mix of antibodies against Mdm2 that recognize different domains within the protein. F RhoA protein levels in HT1080 p53KO cells transfected with siRNAs against Mdm2 alone or against Mdm2 and Spry4. α-tubulin was used as a loading control. G Quantification of wound scratch migration assay comparing cells transfected with siRNAs against Mdm2 alone or against Mdm2 and a pool of Spry4 siRNAs in the presence or absence of the RhoA inhibitor Rhosin (50 µM) for 24 h. The graph represents mean ± SD, n = 3 technical replicates. The graph for the other two independent experimental replicates can be found in the supplementary material. H Immunoblot of levels of total and phospho-cofilin-1(Ser3) in HT1080 p53KO cells silenced for Mdm2 alone or with double KD of Mdm2 and Spry4. I Immunofluorescence showing F- (red) and G-actin (green) staining. Nuclei (blue) as detected by DAPI staining, n = 3 groups. Representative images (left) and quantification of F/G ratio (right). Graph represents mean ± SD of independent experimental replicates, in each replicate the F/G-actin ratio was quantified in at least 30 events/condition for a total of at least 90 events/condition. More details about the statistical tests used can be found in the Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-51488-2'>39164253</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-spry4-primary-antibodies-ihc-testing-2.jpgAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband® IHC analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). <br>
Sprouty 4/Spry-4/SPRY4 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Sprouty 4/Spry-4/SPRY4 Antibody (A04343-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-spry4-primary-antibodies-ihc-testing-3.jpgAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband® IHC analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). <br>
Sprouty 4/Spry-4/SPRY4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Sprouty 4/Spry-4/SPRY4 Antibody (A04343-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-spry4-primary-antibodies-ihc-testing-4.jpgAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband® IHC analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). <br>
Sprouty 4/Spry-4/SPRY4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Sprouty 4/Spry-4/SPRY4 Antibody (A04343-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-2-spry4-primary-antibodies-fcm-testing-5.jpgAnti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2).<br>Overlay histogram showing PC-3 cells stained with A04343-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sprouty 4/Spry-4/SPRY4 Antibody (A04343-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-trim21-ss-a-picoband-trade-antibody-a02079-2-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02079-2-trim21-primary-antibodies-wb-testing-1.jpgAnti-TRIM21/SS-A Antibody Picoband® Western blot analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat lung tissue lysates, <br>
Lane 2: mouse lung tissue lysates,<br>
Lane 3: rat thymus tissue lysates,<br>
Lane 4: mouse thymus tissue lysates,<br>
Lane 5: human Hela whole cell lysates,<br>
Lane 6: human THP-1 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM21/SS-A antigen affinity purified polyclonal antibody (Catalog # A02079-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM21/SS-A at approximately 52KD. The expected band size for TRIM21/SS-A is at 52KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02079-2-trim21-primary-antibodies-if-testing-2.jpgAnti-TRIM21/SS-A Antibody Picoband® IF analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2). <br>
TRIM21/SS-A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRIM21/SS-A Antibody (A02079-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02079-2-trim21-primary-antibodies-fcm-testing-3.pngAnti-TRIM21/SS-A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TRIM21/SS-A antibody (A02079-2).<br>Overlay histogram showing SiHa cells stained with A02079-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM21/SS-A Antibody (A02079-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-trim25-efp-picoband-trade-antibody-a03232-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-wb-testing-1.jpgAnti-TRIM25/EFP Antibody Picoband® Western blot analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HepG2 whole cell lysates, <br>
Lane 2: human Hela whole cell lysates, <br>
Lane 3: human K562 whole cell lysates, <br>
Lane 4: human Caco-2 whole cell lysates, <br>
Lane 5: human T-47D whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified polyclonal antibody (Catalog # A03232-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM25 at approximately 71KD. The expected band size for TRIM25 is at 71KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-ihc-testing-2.jpgAnti-TRIM25/EFP Antibody Picoband® IHC analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). <br>
TRIM25 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM25 Antibody (A03232-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-ihc-testing-3.jpgAnti-TRIM25/EFP Antibody Picoband® IHC analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). <br>
TRIM25 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM25 Antibody (A03232-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-ihc-testing-4.jpgAnti-TRIM25/EFP Antibody Picoband® IHC analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). <br>
TRIM25 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRIM25 Antibody (A03232-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-if-testing-5_.jpgAnti-TRIM25/EFP Antibody Picoband® IF analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). <br>
TRIM25 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TRIM25 Antibody (A03232-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03232-1-trim25-primary-antibodies-fc-testing-6.pngAnti-TRIM25/EFP Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TRIM25 antibody (A03232-1). <br>Overlay histogram showing U937 cells stained with A03232-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM25 Antibody (A03232-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-txnip-picoband-trade-antibody-a01409-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-tlcr-11-07-1380-f6.jpgAnti-TXNIP Antibody Picoband®Immune cell infiltration analysis. Correlation analysis of immune cell infiltration level with expression levels of prognostic ferroptosis-related genes (A) and TXNIP gene (B). TXNIP, thioredoxin-interacting protein; NK, natural killer cells; DC, dendritic cells; MAIT, mucosal-associated invariant T cells; NKT, natural killer T cells; Corr, correlation coefficient.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9359951/&orig_host=www.ncbi.nlm.nih.gov'>35958330</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-tlcr-11-07-1380-f7.jpgAnti-TXNIP Antibody Picoband®Survival and anti-cancer drug sensitivity analysis of TXNIP. (A) SCLC patients were divided into the TXNIP-low group and the TXNIP-high group. (B) Comparison of survival curves between the TXNIP-low and TXNIP-high group. (C) Volcano map exhibiting differences of anti-cancer drugs’ IC50 values in TXNIP-high group and TXNIP-low group of SCLC cell lines. IC50 values of AMG-319 (D) and Topotecan (E) in the TXNIP-high group and the TXNIP-low group. *, P<0.05; ***, P<0.001; ****, P<0.0001. TXNIP, thioredoxin-interacting protein; SCLC, small cell lung cancer; IC50, 50% inhibition concentration; AMG-319, an inhibitor of phosphoinositide-3 kinase; Topotecan, a topoisomerase I inhibitor.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9359951/&orig_host=www.ncbi.nlm.nih.gov'>35958330</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-txnip-primary-antibodies-wb-testing-1.jpgAnti-TXNIP Antibody Picoband® Western blot analysis of TXNIP using anti-TXNIP antibody (A01409-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat brain tissue lysates, <br>
Lane 2: rat kidney tissue lysates, <br>
Lane 3: rat PC-12 whole cell lysates, <br>
Lane 4: mouse brain tissue lysates, <br>
Lane 5: mouse kidney tissue lysates, <br>
Lane 6: mouse NIH-3T3 whole cell lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates, <br>
Lane 8: human K562 whole cell lysates, <br>
Lane 9: human Hela whole cell lysates, <br>
Lane 10: human HEK293 whole cell lysates, <br>
Lane 11: human HL-60 whole cell lysates, <br>
Lane 12: human A549 whole cell lysates, <br>
Lane 13: human Caco-2 whole cell lysates, <br>
Lane 14: human Raji whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNIP antigen affinity purified polyclonal antibody (Catalog # A01409-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNIP at approximately 44KD. The expected band size for TXNIP is at 44KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-tlcr-11-07-1380-f8.jpgAnti-TXNIP Antibody Picoband®Analysis of TXNIP and immunotherapy efficacy. The expression levels of TXNIP (A) and PD-L1 (B) in the response and non-response groups. (C) Immunohistochemical staining of the tumor specimen from patients in response group and non-response group. *, P<0.05. ns, no significance; SCLC, small cell lung cancer; TXNIP, thioredoxin-interacting protein; PD-L1, programmed cell death ligand 1.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9359951/&orig_host=www.ncbi.nlm.nih.gov'>35958330</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-txnip-primary-antibodies-ihc-testing-2.jpgAnti-TXNIP Antibody Picoband® IHC analysis of TXNIP using anti-TXNIP antibody (A01409-1). <br>
TXNIP was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXNIP Antibody (A01409-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01409-1-txnip-primary-antibodies-fc-testing-3.pngAnti-TXNIP Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TXNIP antibody (A01409-1). <br>Overlay histogram showing THP-1 cells stained with A01409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNIP Antibody (A01409-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-u2af65-u2af2-picoband-trade-antibody-a03639-2-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-wb-testing-1.jpgAnti-U2AF65/U2AF2 Antibody Picoband® Western blot analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human THP-1 whole cell lysates, <br>
Lane 3: human U2OS whole cell lysates, <br>
Lane 4: rat brain tissue lysates, <br>
Lane 5: rat spleen tissue lysates, <br>
Lane 6: mouse brain tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U2AF2 antigen affinity purified polyclonal antibody (Catalog # A03639-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U2AF2 at approximately 65KD. The expected band size for U2AF2 is at 65KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-ihc-testing-2.jpgAnti-U2AF65/U2AF2 Antibody Picoband® IHC analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). <br>
U2AF2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-U2AF2 Antibody (A03639-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-ihc-testing-3.jpgAnti-U2AF65/U2AF2 Antibody Picoband® IHC analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). <br>
U2AF2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-U2AF2 Antibody (A03639-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-ihc-testing-4.jpgAnti-U2AF65/U2AF2 Antibody Picoband® IHC analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). <br>
U2AF2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-U2AF2 Antibody (A03639-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-if-testing-5.jpgAnti-U2AF65/U2AF2 Antibody Picoband® IF analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). <br>
U2AF2 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-U2AF2 Antibody (A03639-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-ip-testing-1.jpgAnti-U2AF65/U2AF2 Antibody Picoband®Immunoprecipitating U2AF2 in A549 whole cell lysate.<br>
Western blot analysis of U2AF2 using anti-U2AF2 antibody (A03639-2).<br>
Lane 1: A549 whole cell lysates (30ug),<br>
Lane 2: Rabbit control IgG instead of anti-U2AF2 antibody in A549 whole cell lysate,<br>
Lane 3: anti-U2AF2 antibody (2μg) + A549 whole cell lysate (500μg).<br>
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-U2AF2 antigen affinity purified polyclonal antibody (A03639-2) at a dilution of 1:50 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for U2AF2 at approximately 65 kDa. The expected band size for U2AF2 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-fc-testing-6.pngAnti-U2AF65/U2AF2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-U2AF2 antibody (A03639-2). <br>Overlay histogram showing PC-3 cells stained with A03639-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-U2AF2 Antibody (A03639-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-fc-testing-7.pngAnti-U2AF65/U2AF2 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-U2AF2 antibody (A03639-2). <br>Overlay histogram showing ANA-1 cells stained with A03639-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-U2AF2 Antibody (A03639-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03639-2-u2af2-primary-antibodies-fc-testing-8_1.pngAnti-U2AF65/U2AF2 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-U2AF2 antibody (A03639-2). <br>Overlay histogram showing NRK cells stained with A03639-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-U2AF2 Antibody (A03639-2, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-ulk1-picoband-trade-antibody-a00584-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-ulk1-primary-antibodies-wb-testing-1.jpgAnti-ULK1 Antibody Picoband® Western blot analysis of ULK1 using anti-ULK1 antibody (A00584-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human A549 whole cell lysates, <br>
Lane 2: human THP-1 whole cell lysates,<br>
Lane 3: rat brain tissue lysates,<br>
Lane 4: mouse brain tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK1 antigen affinity purified polyclonal antibody (Catalog # A00584-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK1 at approximately 120KD. The expected band size for ULK1 is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-41419_2024_7120_fig6_html.pngAnti-ULK1 Antibody Picoband®FBXO16 interacts with ULK1 and facilitates its K48 linked poly-ubiquitination. a Schematic presentation of predicted interaction between ULK1 and CUL1-SKP1-FBXO16 complex. Co-IP analysis with anti-Flag antibody to detect the interaction between Flag-FBXO16 and Myc-ULK1 ( b ), and Myc-FBXO16 and Flag-ULK1 ( c ) in HEK293T cells co-transfected with the indicated plasmids. d Lysates from OVCAR3 cells were subjected to immunoprecipitation analysis with anti-FBXO16 antibody, followed by immunoblotting analysis with anti-FBXO16 and anti-ULK1 antibodies, respectively. e Recombinant FBXO16 and ULK1 proteins were prepared in an in vitro transcription and translation system, immunoprecipitation analysis was performed using anti-FBXO16 antibody, immunoblot analysis was followed with anti-ULK1 antibody. Wild type (WT) and truncated mutant schematic structures of ULK1 ( f ) and FBXO16 ( g ) were presented (upper), and co-IP with Flag-antibody was conducted to visualize the interaction between FBXO16 with ULK1 truncations (lower), and ULK1 with FBXO16 truncated mutants (lower). OVCAR3 ( h ) and A2780 ( i ) cells were infected with lenti-virus to over-express FBXO16 (WT) and FBXO16 (∆CTD) proteins, and CCK8 assay was performed to detect the proliferation of both cells. Co-IP with anti-Flag antibody followed by immunoblot analysis (IB) to detect ULK1 ubiquitination affected by FBXO16 in HEK293T cells. WT, K48, and K63 mutant forms of HA-Ubs were used in ( j ), while WT and K48R mutant of HA-Ubs were used in ( k ). Similar results were obtained in three independent experiments. l Recombinant ULK1 and FBXO16 proteins were prepared in an in vitro transcription and translation system. In vitro ubiquitination assay was performed in the presence of E1, E2, FBXO16, ULK1, Ubs (WT, K48, K63), and immunoprecipitated CUL1-SKP1-RBX1 complex. The ubiquitination of ULK1 was examined by immunoblot analysis with anti-ULK1 antibody. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-07120-8'>39384743</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-41419_2024_7120_fig7_html.pngAnti-ULK1 Antibody Picoband®Clinical MIR937 amplification correlates with FBXO16 and ULK1 expression. a IB analysis of protein levels for ULK1, P62, LC3II/I in MIR937 +/+ , MIR937 +/− , and MIR937 −/− OVCAR3 (upper) and A2780 (lower) cells. GAPDH was used as loading control. b IB analysis for comparing FBXO16, ULK1 protein abundances in clinical OV with control samples. Ctrl: normal ovary, MIR937 WT: OV patient samples without amplification of MIR937 . MIR937 AMP: OV patient samples with MIR937 amplification. c Representative images for IHC analysis of ULK1 protein in OV patient samples or the normal control, Scale bar: 100 μm. Kaplan–Meier survival analysis of OV patients from GSE26193 dataset grouped by FBXO16 ( d ) and ULK1 ( e ) expression. f Kaplan–Meier survival analysis of OV patients grouped by both FBXO16 and ULK1 expression in GSE26193. Similar results were obtained in three independent experiments. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-07120-8'>39384743</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-ulk1-primary-antibodies-ihc-testing-2.jpgAnti-ULK1 Antibody Picoband® IHC analysis of ULK1 using anti-ULK1 antibody (A00584-1). <br>
ULK1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ULK1 Antibody (A00584-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-ulk1-primary-antibodies-ihc-testing-3.jpgAnti-ULK1 Antibody Picoband® IHC analysis of ULK1 using anti-ULK1 antibody (A00584-1). <br>
ULK1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ULK1 Antibody (A00584-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00584-1-ulk1-primary-antibodies-fcm-testing-4.pngAnti-ULK1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ULK1 antibody (A00584-1).<br>Overlay histogram showing A549 cells stained with A00584-1 (Blue line).To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ULK1 Antibody (A00584-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-usp15-picoband-trade-antibody-a03057-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03057-1-usp15-primary-antibodies-wb-testing-1.jpgAnti-USP15 Antibody Picoband® Western blot analysis of USP15 using anti-USP15 antibody (A03057-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP15 antigen affinity purified polyclonal antibody (Catalog # A03057-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP15 at approximately 112KD. The expected band size for USP15 is at 112KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03057-1-usp15-primary-antibodies-if-testing-2.jpgAnti-USP15 Antibody Picoband® IF analysis of USP15 using anti-USP15 antibody (A03057-1). <br>
USP15 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-USP15 Antibody (A03057-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03057-1-usp15-primary-antibodies-fcm-testing-3.pngAnti-USP15 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-USP15 antibody (A03057-1).<br>Overlay histogram showing HL-60 cells stained with A03057-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP15 Antibody (A03057-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-usp21-picoband-trade-antibody-a06639-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06639-1-usp21-primary-antibodies-wb-testing-1.jpgAnti-USP21 Antibody Picoband® Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HELA whole cell lysates, <br>
Lane 2: human HEPG2 whole cell lysates, <br>
Lane 3: human PANC-1 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: rat kidney tissue lysates, <br>
Lane 6: mouse kidney tissue lysates, <br>
Lane 7: mouse RAW264.7 whole cell lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06639-1-usp21-primary-antibodies-if-testing-2.jpgAnti-USP21 Antibody Picoband® IF analysis of USP21 using anti-USP21 antibody (A06639-1). <br>
USP21 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-USP21 Antibody (A06639-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-usp25-picoband-trade-antibody-a06182-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06182-1-usp25-primary-antibodies-wb-testing-1.jpgAnti-USP25 Antibody Picoband® Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human HEK293 whole cell lysates, <br>
Lane 2: human placenta tissue lysates, <br>
Lane 3: monkey liver tissue lysates, <br>
Lane 4: monkey kidney tissue lysates, <br>
Lane 5: human PC-3 whole cell lysates, <br>
Lane 6: human Hela whole cell lysates, <br>
Lane 7: human A549 whole cell lysates, <br>
Lane 8: rat kidney tissue lysates, <br>
Lane 9: rat liver tissue lysates, <br>
Lane 10: rat lung tissue lysates, <br>
Lane 11: human PC-12 whole cell lysates, <br>
Lane 12: mouse lung tissue lysates, <br>
Lane 13: mouse RAW264.7 whole cell lysates, <br>
Lane 14: mouse NIH-3T3 whole cell lysates, <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06182-1-usp25-primary-antibodies-if-testing-2.jpgAnti-USP25 Antibody Picoband® IF analysis of USP25 using anti-USP25 antibody (A06182-1). <br>
USP25 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-USP25 Antibody (A06182-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06182-1-usp25-primary-antibodies-fc-testing-3.pngAnti-USP25 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-USP25 antibody (A06182-1). <br>Overlay histogram showing HL-60 cells stained with A06182-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP25 Antibody (A06182-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-usp44-picoband-trade-antibody-a08401-2-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-usp44-primary-antibodies-wb-testing-1_1.jpgAnti-USP44 Antibody Picoband® Western blot analysis of USP44 using anti-USP44 antibody (A08401-2). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: rat testis tissue lysates, <br>
Lane 2: mouse testis tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP44 antigen affinity purified polyclonal antibody (Catalog # A08401-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP44 at approximately 81KD. The expected band size for USP44 is at 81KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig5_html.pngAnti-USP44 Antibody Picoband®USP44 reduces lung metastasis of OSCC cells in vivo. ( A ) Images of lung tissues from mice. ( B ) Representative H&E staining of lung tissues. ( C-D ) Quantification of pulmonary nodules in histograms <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig1_html.pngAnti-USP44 Antibody Picoband®Downregulation of USP44 in OSCC. ( A ) Volcano plot: all differentially expressed coding mRNAs from the GSE37991 ( ) and The Cancer Genome Atlas (TCGA, ) databases. ( B ) Venn diagrams: differentially expressed genes (DEGs) identified from GSE37991 and TCGA data sets. The overlaps display the up- or down-regulated DEGs common to both datasets. ( C ) Kaplan-Meier survival analysis of OSCC patients in TCGA data sets. ( D and E ) The expression levels of USP44 in OSCC tumor tissues and adjacent normal tissues using data from GSE37991, TCGA and samples from The First Affiliated Hospital of Zhengzhou University, respectively. ( F ) Representative immunohistochemical staining of USP44 in human OSCC tissues <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig2_html.pngAnti-USP44 Antibody Picoband®USP44 inhibits OSCC cell proliferation, migration, and invasion in vitro. ( A-B ) CCK-8 assays showing that cell viability was enhanced in USP44-silenced OSCC cells but was decreased in USP44-overexpressed OSCC cells. ( C-D ) Clonogenic assays depicting the effects of USP44 on OSCC cell proliferation. ( E-F ) Transwell assays analyzing the migration and invasion capabilities of OSCC cells with altered USP44 expression. ( G-H ) Quantitation of Transwell assays in E and F <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig3_html.pngAnti-USP44 Antibody Picoband®USP44 inhibits stemness of OSCC cells. ( A-B ) Representative microscopy images of monolayer and spherical OSCC cells. The mRNA levels of stem cell markers CD133, OCT4, SOX2 and Nanog were detected by RT-qPCR. ( C-D ) The expression of USP44 in monolayer and spherical OSCC cells, respectively, using RT-qPCR and western blotting assays. ( E ) Representative microscopy images of spheroid formation in OSCC cells. ( F ) RT-qPCR quantification of CD133, OCT4, SOX2 and Nanog mRNA levels in the indicated OSCC cells. mc, monolayer cell; sc, spherical cell <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig4_html.pngAnti-USP44 Antibody Picoband®USP44 suppresses tumorigenic abilities of OSCC cells in vivo. ( A-B ) Images of subcutaneous tumors from mice. The tumor growth curves (volume) over the course of the study were also shown. ( C-D ) Representative immunohistochemical staining images of tumor tissues for USP44, CD133, and Ki67 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig7_html.pngAnti-USP44 Antibody Picoband®USP44 enhances the stability of HEXIM protein. ( A ) Western blotting showing the expression of HEXIM in CAL-27 and SCC-9 cells. ( B ) Co-IP with anti-USP44 or anti-HEXIM1 antibody in CAL-27 and SCC-9 cells showing the endogenous interaction between USP44 and HEXIM1. ( C ) Co-IP with anti-Flag or anti-Myc antibody showing the interaction between Flag-USP44 and Myc-HEXIM1 overexpressed in 293T cells. ( D ) Effect of overexpression of USP44 on HEXIM1 stability. CAL-27 cells were transfected with either empty vector or USP44-expressing vector and then treated with CHX for the indicated periods of time. ( E ) Effects of USP44 overexpression on HEXIM1 ubiquitination. 293T cells transfected with Flag-USP44, Myc-HEXIM1 or HA-ubiquitin (Ub) plasmids were subjected to IP with anti-Myc antibody and then immunoblotted with the anti-HA or anti-Myc antibody <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig6_html.pngAnti-USP44 Antibody Picoband®Label-free quantitative proteomics and CO-IP mass spectrometry analyses of USP44 effects in OSCC cells. ( A ) PCA plot illustrating expression data variance between pLJM1-Ctrl and pLJM1-USP44 overexpression in CAL-27 cells. ( B ) Volcano plot showing differentially expressed proteins in OSCC cells by the label-free quantitative proteomics and LC-MS/MS analysis. ( C ) GO (gene ontology) analysis showing the enrichment of biological processes among proteins differentially expressed in pLJM1-Ctrl- versus pLJM1-USP44-CAL-27 cells. ( D ) Circle plot showing KEGG pathway enrichment for differentially expressed proteins in pLJM1-Ctrl- versus pLJM1-USP44-CAL-27 cells. ( E ) Venn diagrams in the left panel comparing the proteins identified from proteomics and CO-IP mass spectrometry analyses, respectively. Overlaps showing the F 112 proteins common to both analyses. Heatmap in the right panel showing expression profiles of the 112 proteins. () GO-BP analysis of the 112 proteins. The 9 most significant biological processes are listed. GO terms are represented by filled circles where size is proportional to the significance. ( G ) The protein-protein interaction (PPI) network analyzed using String ( ). The structure of protein HEXIM1 was downloaded from AlphaFold Protein Structure Database ( ). GO and KEGG pathway analyses were performed using the DAVID databease (Database for Annotation, Visualization and Integrated Discovery (DAVID, ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-13062_2024_573_fig8_html.pngAnti-USP44 Antibody Picoband®HEXIM1 mediates the antitumor effects of USP44 in OSCC cells. ( A ) Expression of HEXIM1 in CAL-27 cells transfected with the indicated shRNA plasmids and detected using Western blotting and RT-qPCR, respectively. ( B ) CCK-8 assays showing that cell viability was enhanced in HEXIM1-silenced CAL-27 cells. ( C ) Transwell assays analyzing the migration and invasion capabilities of CAL-27 cells with HEXIM1 silence. Quantification of migration and invasion cells was shown in the histograms. ( D ) Effect of HEXIM1 silencing on cell viability in CAL-27 cells overexpressing USP44. Cells were transfected with the indicated plasmids and the viability was determined by CCK-8 assay. ( E ) Effect of HEXIM1 silencing on cell migration and invasion capabilities in CAL-27 cells overexpressing USP44. Cells were transfected with the indicated plasmids. The migration and invasion capabilities were determined using Transwell assays. Quantification of migration and invasion cells was shown in the histograms <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-024-00573-z'>39722007</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-usp44-primary-antibodies-ihc-testing-2_1.jpgAnti-USP44 Antibody Picoband® IHC analysis of USP44 using anti-USP44 antibody (A08401-2). <br>
USP44 was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-USP44 Antibody (A08401-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-usp44-primary-antibodies-ihc-testing-3.jpgAnti-USP44 Antibody Picoband® IHC analysis of USP44 using anti-USP44 antibody (A08401-2). <br>
USP44 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-USP44 Antibody (A08401-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-usp44-primary-antibodies-fcm-testing-4.jpgAnti-USP44 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-USP44 antibody (A08401-2).<br>Overlay histogram showing A431 cells stained with A08401-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP44 Antibody (A08401-2,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08401-2-usp44-primary-antibodies-if-testing-5.jpgAnti-USP44 Antibody Picoband® IF analysis of USP44 using anti-USP44 antibody (A08401-2). <br>
USP44 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-USP44 Antibody (A08401-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/products/primary-antibodies/anti-vinculin-vcl-picoband-trade-antibody-a01207-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-wb-testing-1_1.jpgAnti-Vinculin/VCL Antibody Picoband® Western blot analysis of VCL using anti-VCL antibody (A01207-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human U-87MG whole cell lysates, <br>
Lane 3: human HT1080 whole cell lysates, <br>
Lane 4: human HEK293 whole cell lysates, <br>
Lane 5: human SK-OV-3 whole cell lysates, <br>
Lane 6: monkey COS-7 whole cell lysates, <br>
Lane 7: human K562 whole cell lysates, <br>
Lane 8: human SGC-7901 whole cell lysates, <br>
Lane 9: rat heart tissue lysates, <br>
Lane 10: mouse heart tissue lysates. <br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-ihc-testing-2_1.jpgAnti-Vinculin/VCL Antibody Picoband® IHC analysis of VCL using anti-VCL antibody (A01207-1). <br>
VCL was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCL Antibody (A01207-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-ihc-testing-3_1.jpgAnti-Vinculin/VCL Antibody Picoband® IHC analysis of VCL using anti-VCL antibody (A01207-1). <br>
VCL was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCL Antibody (A01207-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-ihc-testing-4_1.jpgAnti-Vinculin/VCL Antibody Picoband® IHC analysis of VCL using anti-VCL antibody (A01207-1). <br>
VCL was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCL Antibody (A01207-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-if-testing-5.jpgAnti-Vinculin/VCL Antibody Picoband® IF analysis of VCL using anti-VCL antibody (A01207-1). <br>
VCL was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VCL Antibody (A01207-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01207-1-vcl-primary-antibodies-fc-testing-6_1.pngAnti-Vinculin/VCL Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-VCL antibody (A01207-1). <br>Overlay histogram showing Hela cells stained with A01207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCL Antibody (A01207-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-vps4a-picoband-trade-antibody-a03216-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03216-1-vps4a-primary-antibodies-wb-testing-1.jpgAnti-VPS4A Antibody Picoband® Western blot analysis of VPS4A using anti-VPS4A antibody (A03216-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human K562 whole cell lysates, <br>
Lane 2: human Raji whole cell lysates,<br>
Lane 3: human HEK293 whole cell lysates,<br>
Lane 4: rat brain tissue lysates,<br>
Lane 5: mouse testis tissue lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS4A antigen affinity purified polyclonal antibody (Catalog # A03216-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS4A at approximately 50KD. The expected band size for VPS4A is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03216-1-vps4a-primary-antibodies-ihc-testing-2.jpgAnti-VPS4A Antibody Picoband® IHC analysis of VPS4A using anti-VPS4A antibody (A03216-1). <br>
VPS4A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VPS4A Antibody (A03216-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03216-1-vps4a-primary-antibodies-ihc-testing-3.jpgAnti-VPS4A Antibody Picoband® IHC analysis of VPS4A using anti-VPS4A antibody (A03216-1). <br>
VPS4A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VPS4A Antibody (A03216-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03216-1-vps4a-primary-antibodies-fcm-testing-4.pngAnti-VPS4A Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-VPS4A antibody (A03216-1).<br>Overlay histogram showing A431 cells stained with A03216-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS4A Antibody (A03216-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-vps4b-mig1-picoband-trade-antibody-a03403-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03403-1-vps4b-primary-antibodies-wb-testing-1.jpgAnti-VPS4B/MIG1 Antibody Picoband® Western blot analysis of VPS4B/MIG1 using anti-VPS4B/MIG1 antibody (A03403-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human Hela whole cell lysates, <br>
Lane 2: human HEK293 whole cell lysates,<br>
Lane 3: human Jurkat whole cell lysates,<br>
Lane 4: human SKOV-3 whole cell lysates,<br>
Lane 5: rat kidney tissue lysates,<br>
Lane 6: rat PC-12 whole cell lysates,<br>
Lane 7: mouse brain tissue lysates,<br>
Lane 8: mouse NIH/3T3 whole cell lysates,<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS4B/MIG1 antigen affinity purified polyclonal antibody (Catalog # A03403-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS4B/MIG1 at approximately 50KD. The expected band size for VPS4B/MIG1 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03403-1-vps4b-primary-antibodies-ihc-testing-2.jpgAnti-VPS4B/MIG1 Antibody Picoband® IHC analysis of VPS4B/MIG1 using anti-VPS4B/MIG1 antibody (A03403-1). <br>
VPS4B/MIG1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VPS4B/MIG1 Antibody (A03403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03403-1-vps4b-primary-antibodies-ihc-testing-3.jpgAnti-VPS4B/MIG1 Antibody Picoband® IHC analysis of VPS4B/MIG1 using anti-VPS4B/MIG1 antibody (A03403-1). <br>
VPS4B/MIG1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VPS4B/MIG1 Antibody (A03403-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03403-1-vps4b-primary-antibodies-fcm-testing-4.jpgAnti-VPS4B/MIG1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-VPS4B/MIG1 antibody (A03403-1).<br>Overlay histogram showing A431 cells stained with A03403-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS4B/MIG1 Antibody (A03403-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-rex1-zfp42-picoband-trade-antibody-a08177-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08177-1-zfp42-primary-antibodies-wb-testing-1.jpgAnti-Rex1/ZFP42 Antibody Picoband® Western blot analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). <br>
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>
Lane 1: human placenta tissue lysates, <br>
Lane 2: human Hela whole cell lysates,<br>
Lane 3: human Caco-2 whole cell lysates,<br>
Lane 4: human 22RV1 whole cell lysates.<br>
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rex1/ZFP42 antigen affinity purified polyclonal antibody (Catalog # A08177-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rex1/ZFP42 at approximately 45KD. The expected band size for Rex1/ZFP42 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08177-1-zfp42-primary-antibodies-ihc-testing-2.jpgAnti-Rex1/ZFP42 Antibody Picoband® IHC analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). <br>
Rex1/ZFP42 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rex1/ZFP42 Antibody (A08177-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08177-1-zfp42-primary-antibodies-ihc-testing-3.jpgAnti-Rex1/ZFP42 Antibody Picoband® IHC analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). <br>
Rex1/ZFP42 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rex1/ZFP42 Antibody (A08177-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08177-1-zfp42-primary-antibodies-ihc-testing-4.jpgAnti-Rex1/ZFP42 Antibody Picoband® IHC analysis of Rex1/ZFP42 using anti-Rex1/ZFP42 antibody (A08177-1). <br>
Rex1/ZFP42 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rex1/ZFP42 Antibody (A08177-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08177-1-zfp42-primary-antibodies-fcm-testing-5.pngAnti-Rex1/ZFP42 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Rex1/ZFP42 antibody (A08177-1).<br>Overlay histogram showing A431 cells stained with A08177-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rex1/ZFP42 Antibody (A08177-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
https://www.bosterbio.com/products/primary-antibodies/anti-irak-irak1-antibody-a01021-2-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-1.jpgAnti-IRAK IRAK1 Antibody<strong>Western Blot Validation in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IRAK A01021-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-2.jpgAnti-IRAK IRAK1 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IRAK A01021-2 (1 μg/mL), IRAK 64-231 (2 μg/mL), beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-3.jpgAnti-IRAK IRAK1 Antibody<strong>Western Blot Validation with Recombinant Protein</strong><br>
Loading: 30 ng of human IRAK recombinant protein per lane.
Antibodies: IRAK A01021-2 (1: 1 μg/mL, 2: 2 μg/mL and 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-4.jpgAnti-IRAK IRAK1 Antibody<strong>Species Activity in Mouse and Rat Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IRAK A01021-2 (1 μg/mL,), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-if-testing-5.jpgAnti-IRAK IRAK1 Antibody<strong>Immunofluorescence Validation of IRAK in Human HeLa Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with A01021-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-icc-testing-6.jpgAnti-IRAK IRAK1 Antibody<strong> Immunocytochemistry Validation of IRAK in Human HeLa Cells</strong><br>
Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (A01021-2) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-7.jpgAnti-IRAK IRAK1 Antibody<strong>Immunoprecipitation and Overexpression Validation in HEK293T Cells(Schauvliege et al., 2006)</strong><br>
Co-expression of Pellino proteins and IRAK-1 leads to Pellino phosphorylation and IRAK-1 polyubiquitination. (A) E-tagged Pellino proteins were co-expressed with IRAK-1WT and HA–ubiquitin in HEK293T cells. For assessment of IRAK-1 polyubiquitination, the same cell
extracts, untreated or treated with phosphatase as described above, were analysed for slower migrating forms of IRAK-1 by Western blotting with
anti-IRAK-1 (A01021-2). Ubiquitination was specifically detected by IRAK-1 immunoprecipitation followed by Western blotting with anti-HA antibodies.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01021-2-irak1-primary-antibodies-wb-testing-8.jpgAnti-IRAK IRAK1 Antibody<strong>KD Validation in Human Chondrocytes (Ahmad et al., 2007)</strong><br>
Chondrocytes were transfected with 250 nM of IRAK1 or control siRNA for 48 h and lysates were analyzed for IRAK1 or β-actin expression levels by immunoblotting. IRAK1 signal was disrupted in IRAK1 KD lysate.
https://www.bosterbio.com/products/primary-antibodies/anti-cxcr4-antibody-a00031-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-1.jpgAnti-CXCR4 Antibody<strong>Western Blot Validation of CXCR4 in HeLa Cells </strong><br> Loading: 15 μg of lysates per lane. Antibodies: A00031-1 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-2.jpgAnti-CXCR4 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: A00031-1 (1 μg/mL), 1012 (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-3.jpgAnti-CXCR4 Antibody<strong>Validation with CXCR4 siRNA Knockdown in HeLa Cells</strong><br> HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: A00031-1 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-4.jpgAnti-CXCR4 Antibody<strong>Animal Species Reactivity </strong><br> Loading: Lysates/proteins at 20 μg per lane. Antibodies: A00031-1 (2 μg/mL) or 1012 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-5.jpgAnti-CXCR4 Antibody<strong>Recombinant Protein Test </strong><br> Loading: CXCR4 partial recombinant protein (Novus Biologicals, Cat# H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-if-testing-6.jpgAnti-CXCR4 Antibody<strong>Immunofluorescence Validation of CXCR4 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with A00031-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-fcm-testing-7.jpgAnti-CXCR4 Antibody<strong>Flow Cytometry Validation of CXCR4 in HeLa Cells</strong><br> Overlay histogram showing HeLa cells stained with A00031-1 (red line, 1μg/1x106 cells). 1 h incubation at 4˚C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4˚C. <br> <br> Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testig-11.jpgAnti-CXCR4 Antibody<strong>Overexpression Validation of CXCR4 (Kozak et al., 2002) </strong><br> U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-wb-testing-12.jpgAnti-CXCR4 Antibody<strong>WB Validation of CXCR4 in Human Metastatic Melanoma (Scala et al., 2006) </strong><br> CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-ihc-testing-8.jpgAnti-CXCR4 Antibody<strong>Immunohistochemistry Validation of CXCR4 in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (A00031-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-icc-testing-9.jpgAnti-CXCR4 Antibody<strong>Immunocytochemistry Validation of CXCR4 in HeLa Cells </strong><br> Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody (A00031-1) at 2 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00031-1-cxcr4-primary-antibodies-fcm-testing-10.jpgAnti-CXCR4 Antibody<strong>KO Validation of CXCR4 by Flow Cytometry (?demis, et al., 2010) </strong><br> Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4?/? astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).
https://www.bosterbio.com/products/primary-antibodies/anti-raidd-cradd-antibody-a06509-1-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06509-1-cradd-primary-antibodies-wb-testing-1.jpgAnti-RAIDD CRADD AntibodyWestern blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06509-1-cradd-primary-antibodies-icc-testing-2.jpgAnti-RAIDD CRADD AntibodyImmunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06509-1-cradd-primary-antibodies-if-testing-3.jpgAnti-RAIDD CRADD AntibodyImmunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06509-1-cradd-primary-antibodies-if-testing-4.jpgAnti-RAIDD CRADD AntibodyImmunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-dr3-tnfrsf25-antibody-a03227-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-tnfrsf25-primary-antibodies-wb-testing-1.jpgAnti-DR3 TNFRSF25 AntibodyWestern blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-tnfrsf25-primary-antibodies-if-testing-2.jpgAnti-DR3 TNFRSF25 AntibodyImmunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.<br><br>Green: DR3 Antibody (A03227) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-neurturin-nrtn-antibody-a05924-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-wb-testing-1.jpgAnti-Neurturin NRTN AntibodyWestern blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-if-testing-2.jpgAnti-Neurturin NRTN AntibodyImmunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-if-testing-3.jpgAnti-Neurturin NRTN AntibodyImmunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.<br><br>Green: Neuriturin Antibody (A05924) <br> Red: Phylloidin staining<br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-ihc-testing-4.jpgAnti-Neurturin NRTN AntibodyImmunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-ihc-testing-5.jpgAnti-Neurturin NRTN AntibodyImmunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05924-nrtn-primary-antibodies-if-testing-6.jpgAnti-Neurturin NRTN AntibodyImmunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 μg/mL.<br><br>Green: Neuriturin Antibody (A05924) <br> Red: Phylloidin staining<br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-eotaxin-ccl11-antibody-a01438-boster.html2026-03-24T05:26:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01438-ccl11-primary-antibodies-wb-testing-1.jpgAnti-Eotaxin CCL11 AntibodyWestern blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01438-ccl11-primary-antibodies-icc-testing-2.jpgAnti-Eotaxin CCL11 AntibodyImmunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01438-ccl11-primary-antibodies-if-testing-3.jpgAnti-Eotaxin CCL11 AntibodyImmunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-caspase-10-casp10-antibody-a02190-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02190-casp10-primary-antibodies-wb-testing-1.jpgAnti-Caspase-10 CASP10 AntibodyWestern blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole cell lysates with Caspase-10 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02190-casp10-primary-antibodies-if-testing-2.jpgAnti-Caspase-10 CASP10 AntibodyImmunofluorescence of Casp10 in Hela cells with Caspase 10 antibody at 20 μg/mL.<br><br>Green: Caspase-10 Antibody (A02190) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02190-casp10-primary-antibodies-icc-testing-3.jpgAnti-Caspase-10 CASP10 AntibodyImmunocytochemistry of Casp10 in Hela cells with Caspase 10 antibody at 5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-nik-map3k14-antibody-a03822-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03822-map3k14-primary-antibodies-icc-testing-1.jpgAnti-NIK MAP3K14 AntibodyImmunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03822-map3k14-primary-antibodies-if-testing-2.jpgAnti-NIK MAP3K14 AntibodyImmunofluorescence of NIK in Hek293 cells with NIK antibody at 20 μg/mL.<br><br>Green: NIK Antibody (A03822) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-tace-adam17-antibody-a00604-1-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-1.jpgAnti-TACE ADAM17 Antibody<strong>Western Blot Validation of TACE in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TACE (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lanes: HeLa (A,D), Jurkat (B, E), Raji (C,F) in the absence (A-C) or presence (E-F) of blocking peptide.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-ko-testing-2.jpgAnti-TACE ADAM17 Antibody<strong>KO Validation in HeLa Cells</strong><br> Loading: 10 μg of HeLa WT cell lysates or TACE KO cell lysates. Antibodies: TACE A00604-1 (0.25 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-3.jpgAnti-TACE ADAM17 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TACE A00604-1 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-if-testing-4.jpgAnti-TACE ADAM17 Antibody<strong>Immunofluorescence Validation of TACE in HeLa Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with A00604-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-icc-testing-5.jpgAnti-TACE ADAM17 Antibody<strong>Immunocytochemistry Validation of TACE in HeLa Cells</strong><br>
Immunohistochemical analysis of HeLa cells using anti-TACE antibody (A00604-1) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-6.jpgAnti-TACE ADAM17 Antibody<strong>KD Validation of TACE in Monkey COS Cells. (Wang et al., 2006) </strong><br>
COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody (A00604-1). TACE expression levels were markedly reduced in TACE knockdown cell lysate.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-7.jpgAnti-TACE ADAM17 Antibody<strong>KD Validation of TACE in MDA-MB-435 Cells. (McGowan et al., 2007) </strong><br>
ADAM-17 protein expression, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies (A00604-1).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-8.jpgAnti-TACE ADAM17 Antibody<strong>Overexpression Validation of TACE in MCF-7 Cells. (McGowan et al., 2007) </strong><br>
ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 (A00604-1) antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-wb-testing-9.jpgAnti-TACE ADAM17 Antibody<strong>Induced Expression Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002) </strong><br>
Effect of oxygen–glucose deprivation
(OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. Western blot analysis of TACE in homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-if-testing-10.jpgAnti-TACE ADAM17 Antibody<strong>Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002) </strong><br>
Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.
(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-1-adam17-primary-antibodies-if-testig-11.jpgAnti-TACE ADAM17 Antibody<strong>Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005) </strong><br>
Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.
https://www.bosterbio.com/products/primary-antibodies/anti-gfr-alpha-1-gfra1-antibody-a02897-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02897-gfra1-primary-antibodies-wb-testing-1.jpgAnti-GFR alpha 1 GFRA1 AntibodyWestern blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02897-gfra1-primary-antibodies-ihc-testing-2.jpgAnti-GFR alpha 1 GFRA1 AntibodyImmunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02897-gfra1-primary-antibodies-if-testing-3.jpgAnti-GFR alpha 1 GFRA1 AntibodyImmunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 μg/mL.<br><br>Red: GFR alpha 1 Antibody (A02897) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02897-gfra1-primary-antibodies-ihc-testing-4.jpgAnti-GFR alpha 1 GFRA1 AntibodyImmunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-gfr-alpha-2-gfra2-antibody-a06103-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-gfra2-primary-antibodies-wb-testing-1.jpgAnti-GFR alpha 2 GFRA2 AntibodyWestern blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-gfra2-primary-antibodies-icc-testing-2.jpgAnti-GFR alpha 2 GFRA2 AntibodyImmunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-gfr-alpha-3-gfra3-antibody-a07481-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07481-gfra3-primary-antibodies-wb-testing-1.jpgAnti-GFR alpha 3 Gfra3 AntibodyWestern blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07481-gfra3-primary-antibodies-ihc-testing-2.jpgAnti-GFR alpha 3 Gfra3 AntibodyImmunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07481-gfra3-primary-antibodies-if-testing-3.jpgAnti-GFR alpha 3 Gfra3 AntibodyImmunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml. <br><br>Green: GFR alpha 3 antibody (A07481) <br> Red: Phylloidin staining<br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07481-gfra3-primary-antibodies-ihc-testing-4.jpgAnti-GFR alpha 3 Gfra3 AntibodyImmunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-dff45-dffa-antibody-a03671-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03671-dffa-primary-antibodies-wb-testing-1.jpgAnti-DFF45 DFFA AntibodyWestern blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03671-dffa-primary-antibodies-icc-testing-2.jpgAnti-DFF45 DFFA AntibodyImmunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03671-dffa-primary-antibodies-if-testing-3.jpgAnti-DFF45 DFFA AntibodyImmunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 μg/mL.<br><br>Green: DFF45 Antibody (A03671) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-flip-cflar-antibody-a01295-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-cflar-primary-antibodies-wb-testing-1.jpgAnti-FLIP CFLAR AntibodyWestern blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-cflar-primary-antibodies-icc-testing-2.jpgAnti-FLIP CFLAR AntibodyImmunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-dr3-tnfrsf25-antibody-a03227-1-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-1-tnfrsf25-primary-antibodies-wb-testing-1.jpgAnti-DR3 TNFRSF25 AntibodyWestern blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-1-tnfrsf25-primary-antibodies-icc-testing-2.jpgAnti-DR3 TNFRSF25 AntibodyImmunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-1-tnfrsf25-primary-antibodies-if-testing-3.jpgAnti-DR3 TNFRSF25 AntibodyImmunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-1-tnfrsf25-primary-antibodies-if-testing-4.jpgAnti-DR3 TNFRSF25 AntibodyImmunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 μg/mL.<br><br>Green: DR3 Antibody (A03227-1) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03227-1-tnfrsf25-primary-antibodies-icc-testing-5.jpgAnti-DR3 TNFRSF25 AntibodyImmunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-flip-cflar-antibody-a01295-2-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-2-cflar-primary-antibodies-wb-testing-1.jpgAnti-FLIP CFLAR AntibodyWestern blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-2-cflar-primary-antibodies-ihc-testing-2.jpgAnti-FLIP CFLAR AntibodyImmunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-2-cflar-primary-antibodies-if-testing-3.jpgAnti-FLIP CFLAR AntibodyImmunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-2-cflar-primary-antibodies-icc-testing-4.jpgAnti-FLIP CFLAR AntibodyImmunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01295-2-cflar-primary-antibodies-if-testing-5.jpgAnti-FLIP CFLAR AntibodyImmunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.<br><br>Red: FLIP Antibody (A01295-2) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-daxx-antibody-a00439-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00439-daxx-primary-antibodies-wb-testing-1.jpgAnti-Daxx AntibodyWestern blot analysis of Daxx in 293 total cell lysate with Dax antibody at 1 mg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00439-daxx-primary-antibodies-icc-testing-2.jpgAnti-Daxx AntibodyImmunocytochemistry of DAXX in HeLa cells with DAXX antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00439-daxx-primary-antibodies-if-testing-3.jpgAnti-Daxx AntibodyImmunofluorescence of Daxx in Hela cells with Daxx antibody at 20 μg/mL.<br><br>Green: Daxx Antibody (A00439) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-cad-dffb-antibody-a05374-2-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-2-dffb-primary-antibodies-wb-testing-1.jpgAnti-CAD Dffb AntibodyWestern blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-2-dffb-primary-antibodies-ihc-testing-2.jpgAnti-CAD Dffb AntibodyImmunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-2-dffb-primary-antibodies-if-testing-3.jpgAnti-CAD Dffb AntibodyImmunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-2-dffb-primary-antibodies-ihc-testing-4.jpgAnti-CAD Dffb AntibodyImmunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-2-dffb-primary-antibodies-if-testing-5.jpgAnti-CAD Dffb AntibodyImmunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml. <br><br>Green: CAD antibody (A05374-2) <br> Red: Phylloidin staining<br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-apaf1-antibody-a00889-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00889-apaf1-primary-antibodies-wb-testing-1.jpgAnti-Apaf1 AntibodyWestern blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00889-apaf1-primary-antibodies-ihc-testing-2.jpgAnti-Apaf1 AntibodyImmunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00889-apaf1-primary-antibodies-if-testing-3.jpgAnti-Apaf1 AntibodyImmunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-dr5-tnfrsf10b-antibody-a00410-2-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-1.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: DR5 A00410-2, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-2.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Human HepG2 Cells</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: DR5 A00410-2, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: 1 μg/mL
Lane 2: 2 μg/mL
Lane 3: 4 μg/mLhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-3.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Mouse and Rat Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: DR5 A00410-2, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-4.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Mouse Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: DR5 A00410-2, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-5.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Mouse Heart </strong><br>
Loading: 15 μg of lysatesper lane.
Antibodies: DR5 A00410-2, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-6.jpgAnti-DR5 TNFRSF10B Antibody<strong>Western Blot Validation in Rat Skeletal Muscle </strong><br>
Loading: 15 μg of lysate per lane.
Antibodies: DR5 A00410-2, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-if-testing-7.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunofluorescence Validation of DR5 in Human HepG2 Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed human HepG2 cells labeling DR5 with A00410-2 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-if-testing-8.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunofluorescence Validation of DR5 in Human Testis </strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling DR5 with A00410-2 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-if-testing-9.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunofluorescence Validation of DR5 in Mouse Pancreas </strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling DR5 with A00410-2 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-if-testing-10.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunofluorescence Validation of DR5 in Rat Brain </strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain tissue labeling DR5 with A00410-2 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-12.jpgAnti-DR5 TNFRSF10B Antibody<strong>KO Validation of DR5 in HCT116 Cells (Han et al., 2015) </strong><br>
Anti-cancer drug, Carfilzomib (CFZ), induced up-regulation of DR5 and the expression of DR5 was not detected in DR5-KO HCT 116 cell line with anti-DR5 antibodies (A00410-2).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-wb-testing-13.jpgAnti-DR5 TNFRSF10B Antibody<strong>KD Validation of DR5 in MB231 Cells (Rahman et al., 2009) </strong><br>
Western blot analysis with anti-DR5 antibodies was performed for DR5 in MB231 cells transfected with control siRNA or DR5 siRNA. DR5 expression was disrupted after DR5 siRNA knockdown.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-ihc-testig-11.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunohistochemistry Validation of DR5 in Mouse kidney tissue</strong><br>
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (A00410-2) at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-ihc-testing-14.jpgAnti-DR5 TNFRSF10B Antibody<strong>Immunohistochemistry Validation of BIM in Human Colon Tumors (Devetzi et al., 2016) </strong><br>
Protein analysis for DR5 by immunohistochemistry with anti-DR5 antibodies in human colon tumors. Strong immunoreactivity is shown for DR5 in T167 patient with colorectal cancer.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-2-tnfrsf10b-primary-antibodies-ihc-testing-15.jpgAnti-DR5 TNFRSF10B Antibody<strong>Regulated Expression Validation of DR5 in Thyroid Epithelial Cells (Bretz et al., 2002) </strong><br>
Immunostaining with anti-DR5 antibodies shows high levels of DR5 expression in untreated cells and cells treated with each of the three cytokines alone or TNFalpha combined with IL-1b. In contrast, treatment with both IFNg and TNFalpha or all three cytokines greatly reduces DR5 staining. The reduction in staining appears most significant in cytoplasmic regions while some staining is maintained in or around the nucleus.
https://www.bosterbio.com/products/primary-antibodies/anti-ikk-alpha-chuk-antibody-a01918-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01918-chuk-primary-antibodies-wb-testing-1.jpgAnti-IKK alpha CHUK AntibodyWestern blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01918-chuk-primary-antibodies-icc-testing-2.jpgAnti-IKK alpha CHUK AntibodyImmunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01918-chuk-primary-antibodies-if-testing-3.jpgAnti-IKK alpha CHUK AntibodyImmunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01918-chuk-primary-antibodies-if-testing-4.jpgAnti-IKK alpha CHUK AntibodyImmunofluorescence of IKKa in Jurkat cells with IKK alpha antibody at 20 μg/mL.<br><br>Green: IKK alpha Antibody (A01918) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01918-chuk-primary-antibodies-icc-testing-5.jpgAnti-IKK alpha CHUK AntibodyImmunocytochemistry of IKKa in Jurkat cells with IKK alpha antibody at 2 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-adam10-antibody-a00566-2-boster.html2026-03-24T05:26:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-ko-testing-1.jpgAnti-ADAM10 Antibody<strong>ADAM10 KO Validation in MEF Cells </strong><br>
Loading: 10 μg of lysate
Antibodies: ADAM10 A00566-2, 1 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
A00566-2 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-ko-testing-2.jpgAnti-ADAM10 Antibody<strong>ADAM10 KO Validation in 293 Cells </strong><br>
Loading: 15 μg of lysate
Antibodies: ADAM10 A00566-2, 2 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
A00566-2 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-3.jpgAnti-ADAM10 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: ADAM10 A00566-2, 0.5 μg/mL, ADAM10 24-024, 1 μg/mL, and -actin 3779, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-4.jpgAnti-ADAM10 Antibody<strong>WB Validation in Human Cell Lines </strong><br>
Loading: 15 μg of lysate
Antibodies: ADAM10 A00566-2, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
A00566-2 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-5.jpgAnti-ADAM10 Antibody<strong>Western Blot Validation in Mouse Tissues </strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: ADAM10 A00566-2, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
A00566-2 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-6.jpgAnti-ADAM10 Antibody<strong>Western Blot Validation in Rat Tissues</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: ADAM10 A00566-2, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
A00566-2 detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-if-testing-7.jpgAnti-ADAM10 Antibody<strong>Immunofluorescence Validation of ADAM10 in MOLT4 Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed MOLT4 cells labeling ADAM10 with A00566-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-if-testing-10.jpgAnti-ADAM10 Antibody<strong>Immunofluorescence Validation of ADAM10 in Mouse Testis</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling ADAM10 with A00566-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-if-testig-11.jpgAnti-ADAM10 Antibody<strong>Immunofluorescence Validation of ADAM10 in Rat Testis </strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling ADAM10 with A00566-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-if-testing-12.jpgAnti-ADAM10 Antibody<strong>Immunofluorescence Validation of ADAM10 in Rat Thymus</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat thymus labeling ADAM10 with A00566-2 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-ihc-testing-8.jpgAnti-ADAM10 Antibody<strong>Immunohistochemistry Validation of ADAM10 in Human Testis</strong><br>
Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ADAM10 antibody (A00566-2) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-ihc-testing-9.jpgAnti-ADAM10 Antibody<strong>Immunohistochemistry Validation of ADAM10 in Mouse Thymus</strong><br>
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-ADAM10 antibody (A00566-2) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-if-testing-13.jpgAnti-ADAM10 Antibody<strong>Immunofluorescence Validation of ADAM10 in primary cultures of human cerebral vascular smooth muscle cells (HC-VSMCs) (Coma et al., 2008)</strong><br>
Detection of ADAM10 expression by anti-ADAM10 antibodies in HC-VSMC cells under control condition or in the presence of 10μM H2O2 (oxidative stress condition) for 6 h. ADAM10 expression was not affected when exposed to oxidative stress. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-14.jpgAnti-ADAM10 Antibody<strong>Regulated Expression Validation of ADAM10 in Human neuroblastoma (SH-SY5Y) cells (Zimmermann et al., 2004) </strong><br>
Protein expression of ADAM10 detected by anti-ADAM10 CT antibodies in control or donepezil treated SH-SY5Y cells. When treated with donepezil, the expression of mature form of ADAM10 (68kD) was up-regulated in membrane compartment as compared to the down-regulation in intracellular fractions, and was not affected in whole cell homogenate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-2-adam10-primary-antibodies-wb-testing-15.jpgAnti-ADAM10 Antibody<strong>KD Validation of ADAM10 in Human embryonic kidney 293 cells overexpressing the
human APP 695 isoform (HEK/APP) (Gatta et al., 2009) </strong><br>
Western blot analysis of ADAM10 silencing using anti-ADAM10 antibodies in HEK/APP cells. Silencing with ADAM10 siRNA (Ad) significantly decreased ADAM10 expression, and so did with Ferrochelatase siRNA (F) and N-methylprotoporphyrin IX siRNA (N), 67% and 50% reduction respectively
https://www.bosterbio.com/products/primary-antibodies/anti-zipk-dapk3-antibody-a03300-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03300-1-dapk3-primary-antibodies-wb-testing-1.jpgAnti-ZIPK DAPK3 AntibodyWestern blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03300-1-dapk3-primary-antibodies-if-testing-2.jpgAnti-ZIPK DAPK3 AntibodyImmunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03300-1-dapk3-primary-antibodies-icc-testing-3.jpgAnti-ZIPK DAPK3 AntibodyImmunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-caspase-9-casp9-antibody-a00080-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-wb-testing-1.jpgAnti-Caspase-9 CASP9 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: Caspase 9, 2071 (1 μg/mL), Caspase 9, A00080-1 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-wb-testing-2.jpgAnti-Caspase-9 CASP9 Antibody<strong>Western Blot Validation in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-wb-testing-3.jpgAnti-Caspase-9 CASP9 Antibody<strong>Western Blot Validation in Mouse Cell Line</strong><br>
Loading: 15 μg of 3T3/NIH cell lysate.
Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-if-testing-4.jpgAnti-Caspase-9 CASP9 Antibody<strong>Immunofluorescence Validation of Caspase 9 in K562 Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with A00080-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-if-testing-5.jpgAnti-Caspase-9 CASP9 Antibody<strong>Immunofluorescence Validation of Caspase 9 in HeLa Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with A00080-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-1-casp9-primary-antibodies-icc-testing-6.jpgAnti-Caspase-9 CASP9 Antibody<strong>Immunocytochemistry Validation of Caspase 9 in HeLa Cells</strong><br>
Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody (A00080-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/products/primary-antibodies/anti-rick-ripk2-antibody-a00818-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-ripk2-primary-antibodies-wb-testing-1.jpgAnti-RICK RIPK2 AntibodyWestern blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-ripk2-primary-antibodies-icc-testing-2.jpgAnti-RICK RIPK2 AntibodyImmunocytochemistry of RICK in A431 cells with RICK antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-ripk2-primary-antibodies-if-testing-3.jpgAnti-RICK RIPK2 AntibodyImmunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-ripk2-primary-antibodies-if-testing-4.jpgAnti-RICK RIPK2 AntibodyImmunofluorescence of RICK in K562 cells with RICK antibody at 20 μg/mL.<br><br>Red: RICK Antibody (A00818) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-ripk2-primary-antibodies-icc-testing-5.jpgAnti-RICK RIPK2 AntibodyImmunocytochemistry of RICK in K562 cells with RICK antibody at 2.5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-arc-nol3-antibody-a03991-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03991-nol3-primary-antibodies-wb-testing-1.jpgAnti-ARC NOL3 AntibodyWestern blot analysis of ARC in (H) HeLa, (K) K562, and (A) A549 whole cell lysates with ARC antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03991-nol3-primary-antibodies-ihc-testing-2.jpgAnti-ARC NOL3 AntibodyImmunohistochemistry of ARC in human heart tissue with ARC antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03991-nol3-primary-antibodies-ihc-testing-3.jpgAnti-ARC NOL3 AntibodyImmunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-cide-a-antibody-a02671-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-cidea-primary-antibodies-wb-testing-1.jpgAnti-CIDE-A AntibodyWestern blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (A02671P) with CIDE-A antibody at 1:2000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-cidea-primary-antibodies-ihc-testing-2.jpgAnti-CIDE-A AntibodyImmunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-cidea-primary-antibodies-if-testing-3.jpgAnti-CIDE-A AntibodyImmunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-cide-a-antibody-a02671-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-wb-testing-1.jpgAnti-CIDE-A AntibodyWestern blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-ihc-testing-2.jpgAnti-CIDE-A AntibodyImmunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-if-testing-3.jpgAnti-CIDE-A AntibodyImmunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-ihc-testing-4.jpgAnti-CIDE-A AntibodyImmunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-if-testing-5.jpgAnti-CIDE-A AntibodyImmunofluorescence of CIDE-A in human brain tissue with CIDE A antibody at 20 μg/mL.<br><br>Green: CIDE-A Antibody (A02671-1) <br> Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02671-1-cidea-primary-antibodies-ihc-testing-6.jpgAnti-CIDE-A AntibodyImmunohistochemistry of CIDE-A in human brain tissue with CIDE A antibody at 2.5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-c-c-chemokine-receptor-type-8-ccr8-antibody-a03518-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03518-ccr8-primary-antibodies-wb-testing-1.jpgAnti-C-C chemokine receptor type 8 CCR8 AntibodyWestern blot analysis of CCR8 in human spleen lysate with CCR8 antibody at 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/anti-g-protein-coupled-receptor-15-gpr15-antibody-a11493-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11493-gpr15-primary-antibodies-wb-testing-1.jpgAnti-G-protein coupled receptor 15 GPR15 AntibodyWestern blot analysis of GPR15 in human spleen lysate with GPR15 antibody at (A) 0.5 and (B) 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11493-gpr15-primary-antibodies-icc-testing-2.jpgAnti-G-protein coupled receptor 15 GPR15 AntibodyImmunocytochemistry of GPR15 in A431 cells with GPR15 antibody at 5 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-dff40-dffb-antibody-a05374-3-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-3-dffb-primary-antibodies-wb-testing-1.jpgAnti-DFF40 Dffb AntibodyWestern blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-3-dffb-primary-antibodies-icc-testing-2.jpgAnti-DFF40 Dffb AntibodyImmunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-3-dffb-primary-antibodies-if-testing-3.jpgAnti-DFF40 Dffb AntibodyImmunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05374-3-dffb-primary-antibodies-if-testing-4.jpgAnti-DFF40 Dffb AntibodyImmunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/mL.<br><br>Green: DFF40 Antibody (A05374-3) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-ikk-beta-ikbkb-antibody-a00118-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00118-1-ikbkb-primary-antibodies-wb-testing-1.jpgAnti-IKK beta IKBKB AntibodyWestern blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00118-1-ikbkb-primary-antibodies-icc-testing-2.jpgAnti-IKK beta IKBKB AntibodyImmunocytochemistry staining of HeLa cells using IKK beta antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00118-1-ikbkb-primary-antibodies-if-testing-3.jpgAnti-IKK beta IKBKB AntibodyImmunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-il-1racp-il1rap-antibody-a02824-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02824-il1rap-primary-antibodies-wb-testing-1.jpgAnti-IL-1RAcP IL1RAP AntibodyWestern blot analysis of IL-1RAcP in HeLa whole cell lysate with IL-1RAcP antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02824-il1rap-primary-antibodies-icc-testing-2.jpgAnti-IL-1RAcP IL1RAP AntibodyImmunocystochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 2 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02824-il1rap-primary-antibodies-if-testing-3.jpgAnti-IL-1RAcP IL1RAP AntibodyImmunofluorescence of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.<br><br>Red: IL-1RAcP Antibody (A02824) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-amyloid-beta-a4-protein-app-antibody-a00081-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-app-primary-antibodies-ko-testing-1.jpgAnti-Amyloid-beta A4 protein APP Antibody<strong>APP KO Validation in 293T Cells</strong><br> Loading: 10 μg of lysate
Antibodies: APP A00081, 0.5 μg/mL and beta-actin 3779-1301, 1μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-app-primary-antibodies-wb-testing-2.jpgAnti-Amyloid-beta A4 protein APP AntibodyWestern blot analysis of APP in (A) human, (B) mouse, and (C) rat brain tissue lysates with APP antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-app-primary-antibodies-ihc-testing-3.jpgAnti-Amyloid-beta A4 protein APP AntibodyImmunohistochemistry of APP in human brain (Alzheimer's disease) tissue with APP antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-app-primary-antibodies-ihc-testing-4.jpgAnti-Amyloid-beta A4 protein APP AntibodyImmunohistochemistry of APP in human brain tissue with APP antibody at 2.5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-app-primary-antibodies-if-testing-5.jpgAnti-Amyloid-beta A4 protein APP AntibodyImmunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 μg/mL.<br><br>Green: APP Antibody (A00081) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-dcr3-tnfrsf6b-antibody-a04048-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04048-1-tnfrsf6b-primary-antibodies-wb-testing-1.jpgAnti-DcR3 TNFRSF6B AntibodyWestern blot analysis of DcR3 in human heart (H), brain (B), and kidney (K) tissue lysates with DcR3 antibody at 1:500 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04048-1-tnfrsf6b-primary-antibodies-ihc-testing-2.jpgAnti-DcR3 TNFRSF6B AntibodyImmunohistochemistry of DcR3 in human heart tissue with DcR3 antibody at 1 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-sodd-bag4-antibody-a06722-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06722-bag4-primary-antibodies-wb-testing-1.jpgAnti-SODD BAG4 AntibodyWestern blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. A06722P) with SODD antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06722-bag4-primary-antibodies-icc-testing-2.jpgAnti-SODD BAG4 AntibodyImmunocytochemistry of SODD in HeLa cells with SODD antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06722-bag4-primary-antibodies-if-testing-3.jpgAnti-SODD BAG4 AntibodyImmunofluorescence of SODD in Hela cells with SODD antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-drak1-stk17a-antibody-a08436-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-stk17a-primary-antibodies-wb-testing-1.jpgAnti-DRAK1 STK17A AntibodyWestern blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-stk17a-primary-antibodies-icc-testing-2.jpgAnti-DRAK1 STK17A AntibodyImmunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-stk17a-primary-antibodies-if-testing-3.jpgAnti-DRAK1 STK17A AntibodyImmunofluorescence of DRAK1 in Molt cells with DRAK1 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-drak2-stk17b-antibody-a08711-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08711-stk17b-primary-antibodies-wb-testing-1.jpgAnti-DRAK2 STK17B AntibodyWestern blot analysis of DRAK2 in Raji cell lysate with DRAK2 antibody at (A) 1 and B (2) μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08711-stk17b-primary-antibodies-icc-testing-2.jpgAnti-DRAK2 STK17B AntibodyImmunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-dr6-tnfrsf21-antibody-a04348-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04348-tnfrsf21-primary-antibodies-wb-testing-1.jpgAnti-DR6 TNFRSF21 AntibodyWestern blot analysis of DR6 in K562 (1,3) and Raji (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. A04348P) with DR6 antibody at 1:500 dilution.
https://www.bosterbio.com/products/primary-antibodies/anti-bcl-10-antibody-a01616-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01616-bcl10-primary-antibodies-wb-testing-1.jpgAnti-Bcl-10 AntibodyWestern blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (A01616P) with Bcl-10 antibody at 1:500 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01616-bcl10-primary-antibodies-icc-testing-2.jpgAnti-Bcl-10 AntibodyImmunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01616-bcl10-primary-antibodies-if-testing-3.jpgAnti-Bcl-10 AntibodyImmunofluorescence of Bcl-10 in Raji cells with Bcl-10 antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-dcr1-tnfrsf10c-antibody-a04218-1-boster.html2026-03-24T05:26:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04218-1-tnfrsf10c-primary-antibodies-wb-testing-1.jpgAnti-DcR1 TNFRSF10C AntibodyWestern blot analysis of DcR1 in HeLa whole cell lysate with DcR1 antibody (ED) at 1:500 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04218-1-tnfrsf10c-primary-antibodies-icc-testing-2.jpgAnti-DcR1 TNFRSF10C AntibodyImmunocytochemistry of DcR1 in HeLa cells with DcR1 antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04218-1-tnfrsf10c-primary-antibodies-if-testing-3.jpgAnti-DcR1 TNFRSF10C AntibodyImmunofluorescence of DcR1 in Hela cells with DcR1 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-acinus-acin1-antibody-a04743-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04743-acin1-primary-antibodies-wb-testing-1.jpgAnti-Acinus ACIN1 AntibodyWestern blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (CP) at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04743-acin1-primary-antibodies-icc-testing-2.jpgAnti-Acinus ACIN1 AntibodyImmunocytochemistry staining of K562 cells using Acinus antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04743-acin1-primary-antibodies-if-testing-3.jpgAnti-Acinus ACIN1 AntibodyImmunofluorescence of Acinus in Hela cells with Acinus antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04743-acin1-primary-antibodies-if-testing-4.jpgAnti-Acinus ACIN1 AntibodyImmunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.<br><br>Green: Acinus Antibody (A04743) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-baff-tnfsf13b-antibody-a01257-1-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-wb-testing-1.jpgAnti-BAFF TNFSF13B Antibody<strong>Western Blot Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M) </strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: BAFF A01257-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-wb-testing-2.jpgAnti-BAFF TNFSF13B Antibody<strong>Western Blot Validation in Human, Mouse and Rat Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: BAFF A01257-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-wb-testing-3.jpgAnti-BAFF TNFSF13B Antibody<strong>Western Blot Validation
with Recombinant Protein</strong><br>
Loading: 30 ng of human BAFF recombinant protein per lane.
Antibodies: BAFF A01257-1 (Lane 1: 0.25 μg/mL; Lane 2: 0.5 μg/mL and Lane 3: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Observed at around 18kD.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-icc-testing-4.jpgAnti-BAFF TNFSF13B Antibody<strong>Immunocytochemistry Validation of BAFF in HL60 Cells</strong><br>
Immunocytochemical analysis of HL60 cells using anti-BAFF antibody (A01257-1) at 1 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-if-testing-5.jpgAnti-BAFF TNFSF13B Antibody<strong>Immunofluorescence Validation of BAFF in Human Spleen Tissue</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF with A01257-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-wb-testing-6.jpgAnti-BAFF TNFSF13B Antibody<strong> Regulated Expression Validation of BAFF in
Myeloma Patients (Tai et al., 2006) </strong><br>
Immunoblot analysis was performed to monitor protein expression of BAFF with anti-BAFF antibodies in multiple myeloma cells with or without BMSCs. BAFF expression in cocultures at 8hr or 24hr was up-regulated by ~3.5-fold relative to BMSCs alone.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-ihc-testing-7.jpgAnti-BAFF TNFSF13B Antibody<strong> Immunohistochemistry Validation of BAFF in Thyroid of Patients with Graves' Diseases (Campi et al., 2015) </strong><br>
BAFF expression detected by anti-BAFF antibodies (A01257-1) was remarkably increased in thyrocytes from multinodular goiter (C) compared with either Hashimoto's thyroiditis (E) or Graves' disease (G) while no staining was found in normal thyroid tissue (A).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01257-1-tnfsf13b-primary-antibodies-ihc-testing-8.jpgAnti-BAFF TNFSF13B Antibody<strong> Immunohistochemistry Validation of BAFF in Murine Cardiac Transplants at Rejection (Ye et al., 2004) </strong><br>
BAFF expression detected by anti-BAFF antibodies (A01257-1) was upregulated in intragraft leukocytes due to rejection at 7 days after heart transplant.
https://www.bosterbio.com/products/primary-antibodies/anti-dedaf-rybp-antibody-a04316-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04316-rybp-primary-antibodies-wb-testing-1.jpgAnti-DEDAF RYBP AntibodyWestern blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04316-rybp-primary-antibodies-ihc-testing-2.jpgAnti-DEDAF RYBP AntibodyImmunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04316-rybp-primary-antibodies-if-testing-3.jpgAnti-DEDAF RYBP AntibodyImmunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04316-rybp-primary-antibodies-ihc-testing-4.jpgAnti-DEDAF RYBP AntibodyImmunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04316-rybp-primary-antibodies-if-testing-5.jpgAnti-DEDAF RYBP AntibodyImmunofluorescence of DEDAF in mouse liver tissue with DEDAF antibody at 20 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-survivin-birc5-antibody-a00379-1-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00379-1-birc5-primary-antibodies-wb-testing-1.jpgAnti-Survivin BIRC5 Antibody<strong>Western Blot Validation in Human MOLT4 Cell Lysate</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: Survivin A00379-1 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00379-1-birc5-primary-antibodies-wb-testing-2.jpgAnti-Survivin BIRC5 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: Survivin A00379-1 (5 μg/mL), Survivin 2235 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00379-1-birc5-primary-antibodies-icc-testing-3.jpgAnti-Survivin BIRC5 Antibody<strong>Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br>
Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (A00379-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00379-1-birc5-primary-antibodies-icc-testing-4.jpgAnti-Survivin BIRC5 Antibody<strong>Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br>
Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (A00379-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00379-1-birc5-primary-antibodies-ihc-testing-5.jpgAnti-Survivin BIRC5 Antibody<strong>Immunohistochemistry Validation of Survivin in Mouse Brain Tissue</strong><br>
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (A00379-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/products/primary-antibodies/anti-bace-bace1-antibody-a00322-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-wb-testing-1.jpgAnti-BACE BACE1 Antibody<strong> WB Validation in Human Cell Lines </strong><br>
Loading: 10 μg of lysate
Antibodies: BACE, A00322, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-wb-testing-2.jpgAnti-BACE BACE1 Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: BACE A00322 (1 μg/mL), BACE 32-238 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-wb-testing-3.jpgAnti-BACE BACE1 Antibody<strong>WB Validation in Mouse Tissues </strong><br>
Loading: 15 μg of lysate
Antibodies: BACE, A00322, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-ihc-testing-4.jpgAnti-BACE BACE1 Antibody<strong>Immunohistochemistry Validation of BACE in Human Liver</strong><br>
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BACE antibody (A00322) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-if-testing-5.jpgAnti-BACE BACE1 Antibody<strong>Immunofluorescence Validation of BACE in Mouse Liver</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling BACE with A00322 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-wb-testing-6.jpgAnti-BACE BACE1 Antibody<strong>KO and Overexpression Validation of BACE in Human and Mouse Brain and 293 Cells. (Singer et al., 2005) </strong><br>
Western blot analysis of the BACE1 (A00322) antibody's ability to recognize human and murine BACE1. The BACE1 antibody recognized both the mouse and human forms of BACE1. Lanes 1–4 are frontal cortex homogenates from human and mouse brains. Lane 1 is from a neurologically unimpaired aged human control case, lane 2 from a BACE1-deficient mouse, lane 3 from a nontransgenic mouse and lane 4 from hBACE1 transgenic mouse. Lanes 5–7 are lysates from HEK293T cells transfected with a plasmid vector expressing eGFP, mBACE1 and hBACE1, respectively.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-wb-testing-9.jpgAnti-BACE BACE1 Antibody<strong>KO Validation of BACE in MEF Cells (Jo et al., 2010) </strong><br>
Wildtype and BACE ?/? MEFs were exposed to HNE (15_M) for 2 h. BACE1 levels were examined by Western blot with anti-BACE antibodies (A00322).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-ihc-testing-7.jpgAnti-BACE BACE1 Antibody<strong>KD Validation of BACE in Mouse Brain (Singer et al., 2005)</strong><br>
Characterization of the effects of lenti-siBACE1-6 expression in the brains of APP transgenic mice. (a–d) Anti-eGFP immunoreactivity in the hippocampus (the injection site) shows comparable and consistent expression of lenti-siRNA constructs in the dentate gyrus (dg) and stratus polymorphus (sp). (e) Anti-BACE1 immunoreactivity in the hippocampus of nontransgenic mice treated with lenti-siGlut4. (f) Reduced BACE1 immunostaining in the hippocampus of nontransgenic mice treated with lenti-siBACE1-6 vector. (g) Intense BACE1 immunoreactivity in the hippocampus of APP transgenic mice treated with lenti-siGlut4. (h) Reduced BACE1 expression in APP transgenic mice treated with lenti-siBACE1-6 vector. (i,j) Anti-BACE1 reacted with pyramidal cell bodies in the neocortex, which was not injected,
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-if-testing-8.jpgAnti-BACE BACE1 Antibody<strong>KD Validation of BACE in Mouse Brain (Singer et al., 2005)</strong><br>
Immunolabeling patterns of BACE1 expression and the lenti-siRNA distribution. Sections from APP transgenic mice treated with the eGFPtagged lenti siRNA vectors (green) were co-immunolabeled with an antibody against BACE1 (red) and imaged with the LSCM. All sections are from the hippocampus of treated mice. (a–c) Lenti-siBACE1-6–treated mice. Areas within the hippocampus expressing the eGFP tagged vector have reduced BACE1 immunolabeling. (d–f) Mice treated with the eGFP-tagged control lenti-siGlut4 show unchanged expression of BACE1 in the hippocampus. (g–i) Mice treated with a saline vehicle show unchanged expression of BACE1 in the hippocampus..
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-bace1-primary-antibodies-if-testing-10.jpgAnti-BACE BACE1 Antibody<strong>KD Validation of BACE in DRG (Hyun, 2007) </strong><br>
Decreased BACE1 expression in DRG following siRNA3 transfection. DRG neurons were transfected with 1 μg siRNA3 plasmid and incubated for 48 hours in 37°˚C. DRG neurons were stained for BACE1 us?ing the Anti-BACE antibody (ProSci). (a,b) Neurons transfected with the control plas?mid pSUPER-EGFP (green) did not display any changes in BACE1 expression (red). (c,d) DRG neurons transfected with siR?NA3 displayed reduced BACE1 expression in the axon.
https://www.bosterbio.com/products/primary-antibodies/anti-snon-skil-antibody-a04131-1-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-1-skil-primary-antibodies-wb-testing-1.jpgAnti-SnoN SKIL AntibodyWestern blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-1-skil-primary-antibodies-ihc-testing-2.jpgAnti-SnoN SKIL AntibodyImmunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-1-skil-primary-antibodies-if-testing-3.jpgAnti-SnoN SKIL AntibodyImmunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-ski-oncogene-ski-antibody-a01062-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01062-ski-primary-antibodies-wb-testing-1.jpgAnti-Ski oncogene Ski AntibodyWestern blot analysis of Ski in HeLa cell lysate with Ski antibody at (A) 1 and (B) 2 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01062-ski-primary-antibodies-if-testing-2.jpgAnti-Ski oncogene Ski AntibodyImmunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-skip-snw1-antibody-a03854-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03854-snw1-primary-antibodies-wb-testing-1.jpgAnti-SkiP SNW1 AntibodyWestern blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03854-snw1-primary-antibodies-if-testing-2.jpgAnti-SkiP SNW1 AntibodyImmunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-flash-casp8ap2-antibody-a03925-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03925-casp8ap2-primary-antibodies-wb-testing-1.jpgAnti-FLASH CASP8AP2 AntibodyWestern blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03925-casp8ap2-primary-antibodies-icc-testing-2.jpgAnti-FLASH CASP8AP2 AntibodyImmunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03925-casp8ap2-primary-antibodies-if-testing-3.jpgAnti-FLASH CASP8AP2 AntibodyImmunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-toso-fcmr-antibody-a09423-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09423-faim3-primary-antibodies-wb-testing-1.jpgAnti-Toso FCMR AntibodyWestern blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-f1a-alpha-fem1b-antibody-a07467-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07467-fem1b-primary-antibodies-wb-testing-1.jpgAnti-F1A alpha FEM1B AntibodyWestern blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07467-fem1b-primary-antibodies-ihc-testing-2.jpgAnti-F1A alpha FEM1B AntibodyImmunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07467-fem1b-primary-antibodies-if-testing-3.jpgAnti-F1A alpha FEM1B AntibodyImmunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07467-fem1b-primary-antibodies-if-testing-4.jpgAnti-F1A alpha FEM1B AntibodyImmunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.<br><br>Green: F1A alpha Antibody (A07467) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-protein-lifeguard-2-faim2-antibody-a04900-1-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04900-1-faim2-primary-antibodies-wb-testing-1.jpgAnti-Protein lifeguard 2 FAIM2 AntibodyWestern blot analysis of FAIM2 in EL4 cell lysate with FAIM2 antibody at (A) 0.5 and (B) 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04900-1-faim2-primary-antibodies-ihc-testing-2.jpgAnti-Protein lifeguard 2 FAIM2 AntibodyImmunohistochemistry of FAIM2 in mouse brain tissue with FAIM2 antibody at 5 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-asc-pycard-antibody-a00362-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-wb-testing-1.jpgAnti-ASC PYCARD Antibody<strong>Western Blot Validation in Human HL60 Cells</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: ASC A00362, (1 μg/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-wb-testing-2.jpgAnti-ASC PYCARD Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: ASC A00362, (2 μg/mL), ASC 39-001, (2 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit or goat anti-mouse (for ASC 39001) IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-wb-testing-3.jpgAnti-ASC PYCARD Antibody<strong>Western Blot Validation in Human THP-1 Cells</strong><br>
Loading: 15 μg of lysate per lane.
Antibodies: ASC A00362, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-ihc-testing-4.jpgAnti-ASC PYCARD Antibody<strong>Immunohistochemistry Validation of ASC in Human Spleen Tissue </strong><br>
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ASC antibody (A00362) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-if-testing-5.jpgAnti-ASC PYCARD Antibody<strong>Immunofluorescence Validation of ASC in Human Spleen Tissue</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Spleen Tissue labeling ASC with A00362 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-icc-testing-6.jpgAnti-ASC PYCARD Antibody<strong>Immunocytochemistry Validation of ASC in HL60 Cells</strong><br>
Immunocytochemical analysis of HL60 cells using anti-ASC antibody (A00362) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-wb-testing-7.jpgAnti-ASC PYCARD Antibody<strong>KD Validation of ASC in THP-1 Cells (Dowds et al., 2004) </strong><br>
Immunofluorescence analysis with anti-ASC antibodies (A00362) was performed for BIM in 293 cells transfected with GFP siRNA or ASC siRNA. ASC expression was disrupted after ASC siRNA knockdown.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-pycard-primary-antibodies-wb-testing-8.jpgAnti-ASC PYCARD Antibody<strong>Overexpression Validation of ASC in HEK293T Cells (Dowds et al., 2004) </strong><br>
Western blot analysis with anti-ASC antibodies (A00362) was performed for ASC in HEK293T cells transfected with pcDNA3-ASC.
https://www.bosterbio.com/products/primary-antibodies/anti-bnip3l-antibody-a03107-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03107-bnip3l-primary-antibodies-wb-testing-1.jpgAnti-Bnip3L AntibodyWestern blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03107-bnip3l-primary-antibodies-ihc-testing-2.jpgAnti-Bnip3L AntibodyImmunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03107-bnip3l-primary-antibodies-if-testing-3.jpgAnti-Bnip3L AntibodyImmunofluorescence of Bnip3L in Human Kidney tissue with Bnip3L antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-faim-antibody-a10908-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10908-faim-primary-antibodies-wb-testing-1.jpgAnti-FAIM AntibodyWestern blot analysis of FAIM in human spleen tissue lysate with FAIM antibody at (A) 5 and (B) 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-cide-b-antibody-a07404-1-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-wb-testing-1_1.jpgAnti-CIDE-B AntibodyWB Validation in Human Tissues<br>
Loading: 15 μg of lysate Antibodies: CIDE-B, A07404-1, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-wb-testing-2.jpgAnti-CIDE-B AntibodyWB Validation in Mouse Small Intestine<br>
Loading: 15 μg of lysate Antibodies: CIDE-B, A07404-1, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-wb-testing-3.jpgAnti-CIDE-B AntibodyWB Validation in Rat Small Intestine<br>
Loading: 15 μg of lysate Antibodies: CIDE-B, A07404-1, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-if-testing-1.jpgAnti-CIDE-B AntibodyImmunofluorescence Validation of CIDE-B in Human Small Intestine
Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine tissue labeling CIDE-B with A07404-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-if-testing-2.jpgAnti-CIDE-B AntibodyImmunofluorescence Validation of CIDE-B in Mouse Small Intestine
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse small intestine tissue labeling CIDE-B with A07404-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07404-1-cideb-primary-antibodies-if-testing-3_1.jpgAnti-CIDE-B AntibodyImmunofluorescence Validation of CIDE-B in Rat Liver
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver tissue labeling CIDE-B with A07404-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
https://www.bosterbio.com/products/primary-antibodies/anti-dapk2-antibody-a02241-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02241-dapk2-primary-antibodies-wb-testing-1.jpgAnti-DAPK2 AntibodyWestern blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02241-dapk2-primary-antibodies-ihc-testing-2.jpgAnti-DAPK2 AntibodyImmunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-ikk-epsilon-ikbke-antibody-a01816-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-wb-testing-1.jpgAnti-IKK epsilon IKBKE Antibody<strong>Induction Validation in Mouse Cell Line</strong><br>
Loading: 15 μg of Raw264.7 cell lysates per lane.
Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Cells were treated with LPS (0.3 μg/mL) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-wb-testing-2.jpgAnti-IKK epsilon IKBKE Antibody<strong>Western Blot Validation in Human and Mouse Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-wb-testing-3.jpgAnti-IKK epsilon IKBKE Antibody<strong>Western Blot Validation in Mouse Tissue Lysates</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-wb-testing-4.jpgAnti-IKK epsilon IKBKE Antibody<strong>Western Blot Validation in Jurkat Cell Lysate</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IKK epsilon A01816 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-ihc-testing-5.jpgAnti-IKK epsilon IKBKE Antibody<strong>Immunohistochemistry Validation of IKK epsilon in Human Pancreas Tissue </strong><br>
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IKK epsilon antibody (A01816) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01816-ikbke-primary-antibodies-if-testing-6.jpgAnti-IKK epsilon IKBKE Antibody<strong>Immunofluorescence Validation of IKK epsilon in HeLa Cells</strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling IKK epsilon with A01816 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
https://www.bosterbio.com/products/primary-antibodies/anti-ikk-gamma-ikbkg-antibody-a00874-2-boster.html2026-03-24T05:26:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00874-2-ikbkg-primary-antibodies-wb-testing-1.jpgAnti-IKK gamma IKBKG AntibodyWestern blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00874-2-ikbkg-primary-antibodies-icc-testing-2.jpgAnti-IKK gamma IKBKG AntibodyImmunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00874-2-ikbkg-primary-antibodies-if-testing-3.jpgAnti-IKK gamma IKBKG AntibodyImmunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.<br><br>Red: IKK gamma Antibody (A00874-2) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-ikap-elp1-antibody-a31687-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31687-ikbkap-primary-antibodies-wb-testing-1.jpgAnti-IKAP ELP1 AntibodyWestern blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31687-ikbkap-primary-antibodies-icc-testing-2.jpgAnti-IKAP ELP1 AntibodyImmunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31687-ikbkap-primary-antibodies-if-testing-3.jpgAnti-IKAP ELP1 AntibodyImmunofluorescence of IKAP in A20 cells with IKAP antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-tp1-tep1-antibody-a02017-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02017-tep1-primary-antibodies-wb-testing-1.jpgAnti-TP1 TEP1 AntibodyWestern blot analysis of TP1 in human kidney tissue lysate with TP1 antibody at (A) 0.5 and (B) 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02017-tep1-primary-antibodies-ihc-testing-2.jpgAnti-TP1 TEP1 AntibodyImmunohistochemical staining of human lung tissue using TP1 antibody at 2.5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02017-tep1-primary-antibodies-if-testing-3.jpgAnti-TP1 TEP1 AntibodyImmunofluorescence of TP-1 in Human Lung cells with TP-1 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-nak-tbk1-antibody-a00261-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00261-tbk1-primary-antibodies-icc-testing-1.jpgAnti-NAK TBK1 AntibodyImmunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-p53r2-rrm2b-antibody-a03055-1-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03055-1-rrm2b-primary-antibodies-wb-testing-1.jpgAnti-p53R2 RRM2B AntibodyWestern blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03055-1-rrm2b-primary-antibodies-ihc-testing-2.jpgAnti-p53R2 RRM2B AntibodyImmunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03055-1-rrm2b-primary-antibodies-if-testing-3.jpgAnti-p53R2 RRM2B AntibodyImmunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-chk2-chek2-antibody-a00277-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00277-chek2-primary-antibodies-wb-testing-1.jpgAnti-Chk2 CHEK2 AntibodyWestern blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00277-chek2-primary-antibodies-icc-testing-2.jpgAnti-Chk2 CHEK2 AntibodyImmunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00277-chek2-primary-antibodies-if-testing-3.jpgAnti-Chk2 CHEK2 AntibodyImmunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL<br><br>Red: Chk2 Antibody (A00277) <br> Blue: DAPI staining
https://www.bosterbio.com/products/primary-antibodies/anti-taci-tnfrsf13b-antibody-a02952-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-tnfrsf13b-primary-antibodies-wb-testing-1.jpgAnti-TACI TNFRSF13B AntibodyWestern blot analysis of TACI in K562 (A) and U937 (B) cell lysates with TACI antibody at 5 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02952-tnfrsf13b-primary-antibodies-icc-testing-2.jpgAnti-TACI TNFRSF13B AntibodyImmunocytochemistry of TACI in K562 cells with TACI antibody at 2 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-cell-death-regulator-aven-aven-antibody-a08274-1-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08274-1-aven-primary-antibodies-wb-testing-1.jpgAnti-Cell death regulator Aven Aven AntibodyWestern blot analysis of Aven in Raji cell lysate with Aven antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08274-1-aven-primary-antibodies-ihc-testing-2.jpgAnti-Cell death regulator Aven Aven AntibodyImmunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08274-1-aven-primary-antibodies-if-testing-3.jpgAnti-Cell death regulator Aven Aven AntibodyImmunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-noxa-pmaip1-antibody-a02287-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02287-pmaip1-primary-antibodies-wb-testing-1.jpgAnti-Noxa Pmaip1 AntibodyWestern blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02287-pmaip1-primary-antibodies-ihc-testing-2.jpgAnti-Noxa Pmaip1 AntibodyImmunohistochemistry of NOXA in human stomach tissue with NOXA antibody at 2.5 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02287-pmaip1-primary-antibodies-if-testing-3.jpgAnti-Noxa Pmaip1 AntibodyImmunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02287-pmaip1-primary-antibodies-ihc-testing-4.jpgAnti-Noxa Pmaip1 AntibodyImmunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-pid-mta2-antibody-a03073-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-mta2-primary-antibodies-wb-testing-1.jpgAnti-PID MTA2 AntibodyWestern blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-mta2-primary-antibodies-icc-testing-2.jpgAnti-PID MTA2 AntibodyImmunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-mta2-primary-antibodies-if-testing-3.jpgAnti-PID MTA2 AntibodyImmunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03073-mta2-primary-antibodies-ihc-testing-4.jpgAnti-PID MTA2 AntibodyImmunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-mdm2-binding-protein-mtbp-antibody-a08842-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08842-mtbp-primary-antibodies-wb-testing-1.jpgAnti-Mdm2-binding protein MTBP AntibodyWestern blot analysis of MTBP expression in K562 cell lysate with MTBP antibody at (A) 1 and (B) 2 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08842-mtbp-primary-antibodies-icc-testing-2.jpgAnti-Mdm2-binding protein MTBP AntibodyImmunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08842-mtbp-primary-antibodies-if-testing-3.jpgAnti-Mdm2-binding protein MTBP AntibodyImmunofluorescence of MTBP in K562 cells with MTBP antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-p53aip1-tp53aip1-antibody-a13037-1-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13037-1-tp53aip1-primary-antibodies-wb-testing-1.jpgAnti-p53AIP1 TP53AIP1 AntibodyWestern blot analysis of p53AIP1 in HL60 cell lysate with p53AIP1 antibody at (A) 4 and (B) 8 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13037-1-tp53aip1-primary-antibodies-icc-testing-2.jpgAnti-p53AIP1 TP53AIP1 AntibodyImmunocytochemistry of p53AIP1 in HL60 cells with p53AIP1 antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13037-1-tp53aip1-primary-antibodies-if-testing-3.jpgAnti-p53AIP1 TP53AIP1 AntibodyImmunofluorescence of p53AIP1 in HL60 cells with p53AIP1 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-perp-antibody-a03926-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03926-perp-primary-antibodies-wb-testing-1.jpgAnti-PERP Antibody<strong>Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), PERP, 57-777 (2 μg/mL), beta-actin 3779 (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03926-perp-primary-antibodies-wb-testing-2.jpgAnti-PERP Antibody<strong>Western Blot Validation in Human Cell Lines</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03926-perp-primary-antibodies-wb-testing-3.jpgAnti-PERP Antibody<strong>Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: PERP A03926 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03926-perp-primary-antibodies-icc-testing-4.jpgAnti-PERP Antibody<strong>Immunocytochemistry Validation of PERP in A431 Cells</strong><br>
Immunocytochemical analysis of A431 cells using anti-PEPR antibody (A03926) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
https://www.bosterbio.com/products/primary-antibodies/anti-il-21-antibody-a00459-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-wb-testing-1.jpgAnti-IL-21 Antibody<strong>Western Blot Validation in Human, Mouse and Rat Tissues</strong><br>
Loading: 15 μg of lysates per lane.
Antibodies: IL-21 A00459, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-wb-testing-2.jpgAnti-IL-21 Antibody<strong>Western Blot Validation with Recombinant Protein</strong><br>
Loading: 30 ng of human IL-21 recombinant protein per lane.
Antibodies: IL-21 A00459 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-ihc-testing-3.jpgAnti-IL-21 Antibody<strong> Immunohistochemistry Validation of IL-21 in Human Tonsil</strong><br>
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-ihc-testing-4.jpgAnti-IL-21 Antibody<strong> Immunohistochemistry Validation of IL-21 in Mouse Spleen</strong><br>
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-ihc-testing-5.jpgAnti-IL-21 Antibody<strong> Immunohistochemistry Validation of IL-21 in Rat Spleen</strong><br>
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-wb-testing-6.jpgAnti-IL-21 Antibody<strong>Induced Expression Validation of IL-21 expresssion in patients with Crohn's Disease (Monteleone et al, 2007) </strong><br>
Enhanced IL-21 was observed in involved but not uninvolved
CD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (A00459).
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-wb-testing-7.jpgAnti-IL-21 Antibody<strong> Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al., 2013) </strong><br>
(b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT).
(c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control.
Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody.
https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00459-il21-primary-antibodies-wb-testing-8.jpgAnti-IL-21 Antibody<strong>Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al., 2007) </strong><br>
(A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3).
(C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3).
Both WB show IL-21 expression increased in patients with Hp infection.
https://www.bosterbio.com/products/primary-antibodies/anti-mcg10-pcbp4-antibody-a11870-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11870-pcbp4-primary-antibodies-wb-testing-1.jpgAnti-MCG10 PCBP4 AntibodyWestern blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11870-pcbp4-primary-antibodies-icc-testing-2.jpgAnti-MCG10 PCBP4 AntibodyImmunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11870-pcbp4-primary-antibodies-if-testing-3.jpgAnti-MCG10 PCBP4 AntibodyImmunofluorescence of MCG10 in Hela cells with MCG10 antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-tccr-il27ra-antibody-a03654-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03654-il27ra-primary-antibodies-wb-testing-1.jpgAnti-TCCR IL27RA AntibodyWestern blot analysis of TCCR expression in human spleen tissue lysates with TCCR antibody at 1 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03654-il27ra-primary-antibodies-ihc-testing-2.jpgAnti-TCCR IL27RA AntibodyImmunohistochemistry of TCCR in human spleen tissue with TCCR antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03654-il27ra-primary-antibodies-if-testing-3.jpgAnti-TCCR IL27RA AntibodyImmunofluorescence of TCCR in A549 cells with TCCR antibody at 20 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-il-22-receptor-il22ra1-antibody-a05705-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-il22ra1-primary-antibodies-wb-testing-1.jpgAnti-IL-22 Receptor IL22RA1 AntibodyWestern blot analysis of IL-22 Receptor expression in human HepG2 cell lysate with IL-22 Receptor antibody at 1 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-kappab-ras-nkiras1-antibody-a11990-boster.html2026-03-24T05:26:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11990-nkiras1-primary-antibodies-wb-testing-1.jpgAnti-KappaB ras NKIRAS1 AntibodyWestern blot analysis of KappaB ras in RAW 264.7 cell lysate with KappaB ras antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11990-nkiras1-primary-antibodies-ihc-testing-2.jpgAnti-KappaB ras NKIRAS1 AntibodyImmunohistochemistry of KappaB ras in human lymph node tissue with KappaB ras antibody at 1 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-kappab-ras1-nkiras1-antibody-a11990-1-boster.html2026-03-24T05:26:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11990-1-nkiras1-primary-antibodies-wb-testing-1.jpgAnti-KappaB ras1 NKIRAS1 AntibodyWestern blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11990-1-nkiras1-primary-antibodies-icc-testing-2.jpgAnti-KappaB ras1 NKIRAS1 AntibodyImmunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.
https://www.bosterbio.com/products/primary-antibodies/anti-kappab-ras2-nkiras2-antibody-a13450-boster.html2026-03-24T05:26:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13450-nkiras2-primary-antibodies-wb-testing-1.jpgAnti-KappaB ras2 NKIRAS2 AntibodyWestern blot analysis of KappaB ras2 in RAW264.7 cell lysate with KappaB ras2 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
https://www.bosterbio.com/products/primary-antibodies/anti-il-22-receptor-il22ra1-antibody-a05705-1-boster.html2026-03-24T05:26:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-1-il22ra1-primary-antibodies-wb-testing-1.jpgAnti-IL-22 Receptor IL22RA1 AntibodyWestern blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 μg/ml.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-1-il22ra1-primary-antibodies-ihc-testing-2.jpgAnti-IL-22 Receptor IL22RA1 AntibodyImmunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-1-il22ra1-primary-antibodies-if-testing-3.jpgAnti-IL-22 Receptor IL22RA1 AntibodyImmunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-1-il22ra1-primary-antibodies-icc-testing-4.jpgAnti-IL-22 Receptor IL22RA1 AntibodyImmunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05705-1-il22ra1-primary-antibodies-if-testing-5.jpgAnti-IL-22 Receptor IL22RA1 AntibodyImmunofluorescence of IL-22R in rat kidney tissue with IL-22R antibody at 20 μg/ml.
https://www.bosterbio.com/products/primary-antibodies/anti-livin-birc7-antibody-a02577-boster.html2026-03-24T05:26:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02577-birc7-primary-antibodies-wb-testing-1.jpgAnti-Livin BIRC7 AntibodyWestern blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 μg/mL. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02577-birc7-primary-antibodies-ihc-testing-2.jpgAnti-Livin BIRC7 AntibodyImmunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02577-birc7-primary-antibodies-if-testing-3.jpgAnti-Livin BIRC7 AntibodyImmunofluorescence of Livin in Human Small Intestine cells with Livin antibody at 20 μg/mL.