qPCR Thermo Cycler real time PCR system, Azure Cielo™ 3 and 6
Compatible with the SARS-CoV-2/COVID-19 assays. Viral RNA detection.
The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.
Our novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.
Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.
• Flexibility—Engineered for a wide variety of qPCR applications, with high sensitivity and wide dynamic range
• Intelligent workflow—Our user interface allows simple touch screen assay setup. Saved protocols make assay set up easy and reproducible
• Reliability—Cielo’s optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments, giving you confidence in the reliability of the instrument
• Connectivity—No external PC is required to run the instrument
• Data can be transferred via Ethernet, Wi-Fi or USB
QPCR Thermo Cycler Real Time PCR System, Azure Cielo™ 3 And 6 front image
QPCR Thermo Cycler Real Time PCR System, Azure Cielo™ 3 And 6 angled image
QPCR Thermo Cycler Real Time PCR System, Azure Cielo™ 3 And 6 touchscreen interface
Pre-programmed assays allow for easy selection of calibrators, controls and other experiment related parameters.
Customized data reporting that allows the user to choose which data sets are exported and displayed.
Easily Copy/Paste data or graphs onto Paint, Microsoft Office or other
supported applications as needed. Export data in MS-office, PDF or in MIQE preferred RDML (1.0, 1.1, 1.2) format.
Amplification curves for 96 replicates shown a linear plot (Figure A) and a logarithmic plot (Figure B). In each well, 105 copies of GAPDH template were amplified in the presence of GAPDH primers and BioRad™ Sso Advanced SYBR Green Mix®. Average Cq = 19.1, Coefficient of variance (Cv)= 0.002.
Amplification curves are shown for assays of GAPDH in a dilution series of human reference cDNA (n=3) (Figure A). Assays were conducted using BioRad™ Sso Advanced SYBR Green Mix®. Figure B shows the standard curve obtained by plotting Cq values vs the Amount of Template DNA (copies/µl). R2=0.998, efficiency = 99.89%.
After sequential sets of 1000 continuous qPCR experiments, a GAPDH qPCR assay was performed and Cq values were recorded. Average Cq in each assay = 22.4, ±0.01.
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