Product Info Summary
SKU: | FEK1351 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Rat |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit
See all Erythropoietin/EPO products
SKU/Catalog Number
FEK1351
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat EPO/Erythropoietin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1351)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: T44-K226
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1351 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1351 is reactive to EPO in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
FEK1351 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Erythropoietin/EPO
Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK1351-CAP | 96-well plate precoated with anti-Rat Erythropoietin/EPO antibody | 1 |
EK1351-ST | lyophilized recombinant Rat Erythropoietin/EPO standard | 10 ng/tube |
FEK1351-DA | Biotinylated anti-Rat Erythropoietin/EPO detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.000 | 0.008 | 0.016 | 0.066 | 0.162 | 0.449 | 1.420 | 2.290 |
Data Example Images
Click image to see more details
Rat EPO PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 108 | 342 | 1332 | 113 | 322 | 1373 |
Standard deviation | 5.4 | 25.3%9 | 70.59 | 6.66 | 30.26 | 86.49 |
CV (%) | 5% | 7.6% | 5.3% | 5.9% | 9.4% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 108 | 117 | 110 | 115 | 112 | 3.64 | 3.2% |
Sample 2 | 342 | 374 | 386 | 373 | 368 | 16.26 | 4.4% |
Sample 3 | 1332 | 1189 | 1227 | 1391 | 1284 | 80.66 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For EPO (Source: Uniprot.Org, NCBI)
Uniprot ID
P01588
Gene ID
2056
Gene Name
EPO
Full Name
Erythropoietin
Weight
21.307kDa
Superfamily
EPO/TPO family
Alternative Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EPO, check out the EPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (FEK1351)
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