|Description:||Rat Heart Tissue Lysate|
|Applications:||Western blot control (20-40μg/lane)|
|Product Name||Rat Heart Tissue Lysate|
|Description||This cell lysate is prepared from rat heart tissue using Boster's RIPA Lysis Buffer using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.|
|Applications||Western blot control (20-40μg/lane)|
|Quality Control||1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
3. This lysate is used to test many Boster's primary antibodies. See QC images from these antibodies for more details
|Formulation:||The cell lysate is in SDS-PAGE sample buffer, pH 6.8, containing 50mM TRIS-HCl, 2% SDS, 100mM DTT, 10% glycerol, and 0.01% bromophenol blue.|
|Storage||Shipped at 4˚C. Aliquot and store at -20˚C or -80˚C upon delivery. Avoid repeated freezing and thawing.|
Rat Heart Tissue Lysate Images
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Rat Heart Tissue Lysate (package illustration).