Rat SCGB1A1/uteroglobin PicoKine™ Fast ELISA Kit


SKU FEK1636
Size 96wells/kit, with removable strips.
Reactivity Rat
Sample Type cell culture supernates, serum, plasma (heparin, EDTA) and urine.
Sample Volume 100ul per well
Sensitivity <10pg/ml
Assay Range 156pg/ml-10000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Rat SCGB1A1/uteroglobin PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK1636
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detection of rat SCGB1A1. 96wells/kit, with removable strips.
Cite This Product Rat SCGB1A1/uteroglobin PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1636)
Sample Type cell culture supernates, serum, plasma (heparin, EDTA) and urine. Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <10pg/ml
Assay Range 156pg/ml-10000pg/ml
Immunogen Expression system for standard: E.coli, Immunogen sequence: S20-V96
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK1636-CAP 96-well plate precoated with anti-Rat Scgb1a1 antibody 1
FEK1636-ST lyophilized recombinant Rat Scgb1a1 standard 10ng/tube
FEK1636-DA biotinylated anti-Rat Scgb1a1 antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Rat SCGB1A1/uteroglobin PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)015631262512500025000500010000
O.D.0.0420.0750.0990.1540.2630.5221.1352.156

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2201610449024116354849
Standard deviation8.8123.97298.6%410.36140.61374.3%2
CV(%)4%7.7%6.6%4.3%8.6%7.8%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122025825925324716.036.4%
Sample 21610148313801552150685.635.6%
Sample 344904875448748494675186.973.9%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P17559
Gene Name Scgb1a1
Protein Name secretoglobin family 1A member 1
Tissue Specificity Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Alternative Names Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg
Subcellular Localization Secreted.

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Research Areas Rat

*You can search these to find other products in these research areas.
Background SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

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FEK1636
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