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Product Info Summary
| SKU: | LS006041 |
|---|---|
| Size: | 100 ug |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Antigen standard for ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) (transient overexpression lysate) |
Product info
Product Name
RNase L (RNASEL) Human Over-expression Lysates
SKU/Catalog Number
LS006041
Size
100 ug
Description
Antigen standard for ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL) (transient overexpression lysate)
Storage & Handling
Cite This Product
RNase L (RNASEL) Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006041)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
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