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SKU AR0138
Pack size 1kit (Enough for 30-50 pieces of gel.)
Description Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent.

List of Components

DescriptionQuantityVo lumeCatalog Number
30% Acr-Bis (29:1) 1100mLAR0138-1
1.5M Tris-HCl, pH8.81100mLAR0138-2
10% SDS15mLAR0138-3
Ammonium Persulfate10.5g (Reconstitute to 10% AP solution before use) AR0138-4
TEMED11mLAR0138-5
1M Tris-HCl, pH6.8115mLAR0138-6

Overview

Product Name SDS-PAGE Gel Preparation Kit
SKU/Catalog Number AR0138
Pack Size 1kit (Enough for 30-50 pieces of gel.)
Equivalent N/A
Storage Store 1.5 M Tris-HCl, pH8.8, 10% SDS, Ammonnium persulfate and 1M Tris-HCl, pH 6.8 at room temperature. And store 30% Acr-Bis (29:1) and TEMED at 4°C in dark for 6 months.
Dilute Ammonnium persulfate with DDH2O (double distilled water) at 1:10. Then aliquot it into small tubes, and store at -20°C for 3 months (at 4°C for 1 week).
Description Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent.
Application SDS-PAGE
*Our Boster Guarantee covers the use of this product in the above tested applications.
Cite This Product SDS-PAGE Gel Preparation Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0138)

Assay Principle

Boster’s SDS-PAGE Gel Preparation Kit is convenient to use. It contains various reagents for SDS-PAGE Gel Preparation, only the experimental instruments and DDH2O are needed to prepare. It not only can make up SDS-PAGE gel, but also non-native PAGE gel. This Kit is enough for 30-50 pieces of normal size PAGE gel.

Assay Protocol

1. Choose the proper concentration of gel according to the molecular weight of target protein.
Working range:

Molecular weight of target protein (KDa)Proper gel concentration (%)
>1008
30-10010
10-3012
<1015

2. SDS-PAGE separation gel

Electrophoresis running gel concentration
Reagent8%10%12%15%
DDH2O(ml) 4.634.03.32.3
30%Acr-Bis (T: 30%,C: 3%)(ml) 2.673.34.05.0
1.5mol/lTris.cl(pH8.8) (ml) 2.52.52.52.5
10%SDS(ml) 0.10.10.10.1
10% Ammonium persulfate (ml) 0.10.10.10.1
TEMED(μl)(ml) 4444
Total volume(ml)10

3. SDS-PAGE spacer gel (Concentration: 5%)

ReagentScheme 1Scheme 2
DDH2O(ml)42
30% Acr-Bis (T: 30%,C: 3%(ml) 10.5
1.0mol/lTris.cl(pH6.8) (ml) 10.5
10%SDS(μl)8040
10% Ammonium persulfate (μl) 6030
TEMED(μl)84
Total volume(ml)66

General Notes

•If the room temperature is higher than 30°C, reduce the amount of TEMED by 30-50%.

•If the room temperature is lower than 20°C, increase the amount of TEMED by 30-50%.

SDS-PAGE Gel Preparation Kit Images

Click the images to enlarge.

SDS-PAGE Gel Preparation Kit
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Publications

Effect of CXCR3/HO-1 genes modified bone marrow mesenchymal stem cells on small bowel transplant rejection
Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy
Effect of baicalin on hippocampal damage in kainic acid-induced epileptic mice
Glucosidase II ?-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death
14-3-3? and aPKC-? synergistically facilitate epithelial-mesenchymal transition of cholangiocarcinoma via GSK-3?/Snail signaling pathway
Inhibition of SDF-1?/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis
Expression of hypoxia-inducible factor 3? in hepatocellular carcinoma and its association with other hypoxia-inducible factors
Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment
Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone
Sodium humate accelerates cutaneous wound healing by activating TGF-?/Smads signaling pathway in rats
Molecular Cloning, mRNA Expression, and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis
Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors
Chronic sciatic nerve compression induces fibrosis in dorsal root ganglia
Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells
Immunoregulation of Bone Marrow-Derived Mesenchymal Stem Cells on the Chronic Cigarette Smoking-Induced Lung Inflammation in Rats
Upregulation of Phosphodiesterase type 5 in the Hyperplastic Prostate
Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro
Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma
microRNA-126 suppresses PAK4 expression in ovarian cancer SKOV3 cells
miR-137, a new target for post-stroke depression?
OCT3/4 expression is correlated with the invasion of gastric carcinoma
The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer
Minocycline Attenuates Cognitive Impairment Induced by Isoflurane Anesthesia in Aged Rats
PLK1 gene suppresses cell invasion of undifferentiated thyroid carcinoma through the inhibition of CD44v6, MMP-2 and MMP-9
siRNA targeting decoy receptor 3 enhances the sensitivity of gastric carcinoma cells to 5-fluorouracil
Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
Wang, Z.K., Wang, Z.X., Liu, Z.Y., Ren, Y.Q., & Zhou, Z.Q. (2017). Effects of RNA interference-mediated gene silencing of VEGF on the ultrafiltration failure in a rat model of peritoneal dialysis. Bioscience Reports. Advance online publication. doi: 10.1042/BSR20170342
Chen, W.Q., Xie, Z.Z., Wang, X., Zhao, J.H., Hu, Q., Chen, Y.H.,…, & Liu, Y. (2017). Influences of PON1 on Airway Inflammation and Remodeling in Bronchial Asthma. Journal of Cellular Biochemistry. Advance online publication. doi: 10.1002/jcb.26242
Yin, M.L., Song, H.L., Yang, Y., Zheng, W.P., Liu, T., & Shen, Z.Y. (2017). Effect of CXCR3/HO-1 genes modified bone marrow mesenchymal stem cells on small bowel transplant rejection. World Journal of Gastroenterology, 23(22), 4016-4038. doi: 10.3748/wjg.v23.i22.4016
Zhao, H., Chen, Z., Xie, L.J., & Liu, G.F. (2017). Suppression of TLR4/NF-κB Signaling Pathway Improves Cerebral Ischemia–Reperfusion Injury in Rats. Molecular Neurobiology, 1-9. Advance online publication. doi: 10.1007/s12035-017-0552-0
Ni, X., Ou, C., Guo, J., Liu, B., Zhang, J., Wu, Z.,…, & Chen, M. (2017). Lentiviral vector-mediated co-overexpression of VEGF and Bcl-2 improves mesenchymal stem cell survival and enhances paracrine effects in vitro. International Journal of Molecular Medicine, 1-9. Advance online publication. doi: 10.3892/ijmm.2017.3019
Chen, C.B., Liu, L.S., Zhou, J., Wang, X.P., Han, M., Jiao, X.Y.,…, & Yuan, X.P. (2017). Up-Regulation of HMGB1 Exacerbates Renal Ischemia-Reperfusion Injury by Stimulating Inflammatory and Immune Responses through the TLR4 Signaling Pathway in Mice. Cellular Physiology and Biochemistry, 41(6), 2447-2460. doi: 10.1159/000475914.
Li, X., Han, J., Zhu, H., Peng, L., & Chen, Z. (2017). miR-181b-5p mediates TGF-β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells. International Journal of Oncology, 51(1), 158-168. Advance online publication. doi: 10.3892/ijo.2017.4007
Shang T, Liu L, Zhou J, Zhang M, Hu Q, Fang M, Wu Y, Yao P, Gong Z. Lipids Health Dis. 2017 Mar 29;16(1):65. doi: 10.1186/s12944-017-0461-2. Protective effects of various ratios of DHA/EPA supplementation on high-fat diet-induced liver damage in mice.
Zhipeng Zeng, Ke Wang, Yuanyuan Li, Ni Xia, Shaofang Nie, Bingjie Lv, Min Zhang, Xin Tu, Qianqian Li, Tingting Tang JBC Papers in Press. Published on December 14, 2016 as Manuscript M116.765107 Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy
He XJ, Ding Y, Xiang W, Dang XQ. Cell Physiol Biochem. 2016 Dec 5;40(3-4):743-756 Roles of 1,25(OH)2D3 and Vitamin D Receptor in the Pathogenesis of Rheumatoid Arthritis and Systemic Lupus Erythematosus by Regulating the Activation of CD4+ T Cells and the PKCδ/ERK Signaling Pathway.

Customer Q&As

Question: Is AR0138 suitable for 600kda western blot?
Keywords: recommended gel concentration, large protein, high molecular weight
A: AR0138 is suitable for 600kda western blot. Recommended concentration of separation gel is 6%.