IHC, IF, Histology Services

IHC and IF Services



Boster Bio now offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Save your time and energy by utilizing our state-of-the-art laboratory and expert technicians. Boster guarantees confidential high-quality results for your custom requirements. If you need help with sectioning, embedding, staining, IHC and IF, imaging and analysis, then take the stress out of your research by letting our trusted team of scientists handle it. With 25 years of experience and 20,000+ publications, Boster will give you the best results in customized laboratory services. Get better results with Boster!

Service (Requirements) Description Price per slide
IHC analysis (Paraffin Block) IHC validated protocol, DAB color, primary antibody provided by researcher, we provide secondary antibody $28.75
IHC analysis (TMA Section) IHC validated protocol, DAB color, primary antibody provided by researcher, we provide secondary antibody $115
IF Single-Staining (Paraffin Block/Frozen Section) Single IF validated protocol, red or green color, primary antibody provided by researcher, we provide secondary antibody $51.75
IF Double-Staining (Paraffin block/Frozen Section) Double IF validated protocol, red and green color, primary antibody provided by researcher, we provide secondary antibody $51.75
IF Single Staining (TMA Section) Single IF validated protocol, red or green color, primary antibody provided by researcher, we provide secondary antibody $230
IF Double-Staining (TMA Section) Double IF validated protocol, red and green color, primary antibody provided by researcher, we provide secondary antibody $345
CISH with DAB (Paraffin Block) In situ hybridization and DAB staining $115
CISH with Fluorescence (Paraffin Block) In situ hybridization and fluorescent staining with DAPI $115
TUNEL Bright Field (Paraffin block) TUNEL assay labeled with FITC. Positive apoptosis cells are green, nucleus is blue by DAPI staining $46
TUNEL Fluorescence (Section/Dripping slide) TUNEL assay labeled with FITC. Positive apoptosis cells are green, positive cells labeled by primary antibody are red, nucleus is blue by DAPI staining $46
Bright Field Imaging (Stained Sections) Please specify location which needs to be imaged and magnification factor (1 ~ 400x) $9.20
Bright Field Imaging (TMA Sections) Please specify location which needs to be imaged and magnification factor (1 ~ 400x) $9.20

 

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Histology Sample Preparation



Boster's state-of-the-art lab now offers sample preparation services for histology. Our expert technicians have 25 years of expertise in histology and developing IHC antibodies. Let us know your project requirements and take some worry off your shoulders as our team handles your samples.

Service Description Price
Mouse Bone Decalcification Decalcification of mouse specific bone tissue. Removal of calcium ions from the bone through histological process, thereby making the bone flexible and easy for pathological investigation. It is important to carefully consider the nature of any bone specimen received for processing because the relative amounts of cortical and cancellous bone are important and will determine the time required for decalcification and processing. $28.75
Bone Decalcification Removal of calcium ions from the bone through histological process, thereby making the bone flexible and easy for pathological investigation. It is important to carefully consider the nature of any bone specimen received for processing because the relative amounts of cortical and cancellous bone are important and will determine the time required for decalcification and processing. $40.25

 

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FFPE (formalin-fixed paraffin-embedded) Services



Paraffin-embedding and sectioning are the most widely used methods in morphological research. The usual fixative for paraffin embedded tissues is neutral buffered formalin (NBF). These methods are used to observe the morphological structure and pathological changes of tissues and cells. We provide a full range of services including: paraffin embedding, sectioning and staining, sampling, fixation, dehydration, xylene transparent, paraffin wire, and specialized slicing.

Service Description Price
Tissue processing and Paraffin embedding Tissue is dehydrated through a series of graded ethanol baths, and then infiltrated with paraffin wax. The infiltrated tissues are then embedded into wax blocks. Suitable for all soft tissues and for decalcified bone samples. $11.50
Paraffin Sectioning Sectioning for specimens in paraffin block. 4 μm sections are cut using microtome and prepared on histological slides. $4.60
Semithin Sectioning A microtome is used to cut extremely thin slices of tissue and cell specimens, the semithin section could be 0.6-0.8 μm. $172.50

 

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Cryo (OCT) Services



Cryosectioning is a technique which involves slicing after tissue samples have quickly frozen to a certain hardness. We have adopted the method of cryogenic freezing section to provide frozen sectioning and embedding services. The cryosections are embedded in Optimal Cutting Temperature (OCT) compound, and are preferred over paraffin by some scientists as it preserves the tissue as close as possible to its natural state.

Service Description Price
Cryo embedding (OCT) Embed a fresh or frozen sample into frozen OCT block (optimal cutting temperature compound). $9.20
Cryo sectioning Fixed or fresh tissue samples are cut in 8-10 μm sections in cryostat and mounted on histological slides. $9.20
Semithin Sectioning A microtome is used to cut extremely thin slices of tissue and cell specimens, the semithin section could be 0.6-0.8 μm. $172.50

 

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Staining Services



Based on the goal of an experiment, different staining methods are used to dye the sections, so that various tissue components are stained different colors with dyes via chemical combination or physical adsorption. With our staining services, we provide more than forty staining methods that will suit a wide variety of experiments. Contact us at support@bosterbio.com if you need help with choosing the appropriate staining method!

Service Requirements Price per slide
HE stain Paraffin Slide $5.75
Safranin O-Fast Green Plant paraffin slide $17.25
Safranin O-Fast Green Gristle Paraffin Slide $17.25
Masson Stain Paraffin Slide $17.25
Sirius Red Stain Paraffin Slide $17.25
PAS Stain Paraffin Slide $17.25
Alcian Blue Stain Paraffin Slide $17.25
AB-PAS Stain Paraffin Slide $17.25
Oil red O Stain Frozen Section $17.25
Wright Giemsa Stain Paraffin Slide $17.25
Toluidine Blue Stain Paraffin Slide $17.25
Nissl Stain Paraffin Slide $17.25
Prussian Blue Stain Paraffin Slide $17.25
EVG Stain Paraffin Slide $17.25
VG Stain Paraffin Slide $17.25
Von Kossa Stain Paraffin Slide $17.25
Alizarin Red Stain Paraffin Slide $17.25
Acid-Fast Stain Paraffin Slide $17.25
Feulgen Stain Paraffin Slide $17.25
Weigert Resorcin Fuchsin Stain Paraffin Slide $28.75
Congo Red Stain Paraffin Slide $17.25
TRAP Stain Paraffin Slide $17.25
Silver Stain Paraffin Slide $17.25
PASM Stain Paraffin Slide $17.25
TTC Stain Fresh Tissue $17.25
ALP Stain Frozen Slide $17.25
ATP Stain Frozen Section $17.25
Phloroglucinol Stain Paraffin Section $17.25
Victoria Blue Stain Paraffin Section $17.25
Mast Cell Stain Paraffin Section $17.25

 

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Tissue Microarray (TMA) Services

Boster Bio's tissue microarray service can combine hundreds of tissue samples into one block, which makes large data statistics and analysis more convenient. The researcher should provide tissue paraffin blocks or tissue samples fixed in fixation. The thickness of tissue must be less than 2 mm and researchers should tell us the arrangement of TMA in advance.

Service Description Price per block
within 20×15=300, 1mm A TMA HE staining slide is included $1500
within 16×10=160, 1.5mm A TMA HE staining slide is included $960
within 8×12=96, 2mm A TMA HE staining slide is included $576
within 6×9=45, 3mm A TMA HE staining slide is included $300
within 3×15=15, 5mm A TMA HE staining slide is included $150
HE stain Define the cancer or disease area to punch by pathologists based on HE staining $5 per slide

 

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Electron Microscopy and TEM Services



The electron microscope is a type of microscope that uses a beam of electrons to create an image of the specimen. It is capable of much higher magnifications and has a greater resolving power than a light microscope, allowing it to see much smaller objects in finer detail. Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. Boster provides specialized services for your electron microscopy needs including sample preparation, embedding, sectioning, imaging and analysis.

Service Description Price
Gelatin Embedding Embedding tissues in gelatin makes it possible to cut thin sections for electron microscopy. This method allows us to embed the tissue without passing it through organic solvents extracting the lipid soluble components. Observations made on muscle tissue embedded with this technique have revealed structural components which do not appear after embedding in methacrylate. $23
Resin Embedding For TEM assay of tissue and cell specimens. Resin-infiltration is typically used for transmission electron microscopy. Resin-infiltrated biological samples result in a solid block of resin, which can be further processed by mechanical sectioning, however that does not allow a top view examination of small cell–cell and cell–surface contacts. $230
Semithin Sectioning A microtome is used to cut extremely thin slices of tissue and cell specimens, the semithin section could be 0.6-0.8 μm. $172.50
Ultrathin Sectioning Ultra thin sections of specimens are cut using an ultramicrotome, and can be studied and documented at different magnifications in a transmission electron microscope (TEM). It is used mostly for biological tissue and cell specimens, but sections of plastics and soft metals can also be prepared. Sections must be very thin because the 50 to 125 kV electrons of the standard electron microscope cannot pass through biological material much thicker than 150 nm. For best resolutions, sections should be from 30 to 60 nm. This is roughly the equivalent to splitting a 0.1 mm-thick human hair into 2,000 slices along its diameter, or cutting a single red blood cell into 100 slices. $230

 

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Histology Supplies



Boster provides all the supplies and research reagents you need to perform histology and IHC. Save up to 99% compared to Thermo and Sigma, all products are backed by the Boster Guarantee. Free shipping for qualified products (see *) for orders over $100!

Product Description Price
*Poly-L-Lysine Coated Slides Save time and use pre-coated slides for your immunohistochemistry. Poly-L-Lysine coated slides keeps your cells and tissue sections from peeling off for a fraction of the cost for coating your slides in-house. $19 / 50 slides
*Poly-L-Lysine Solution, 10X Poly-L-Lysine Solution is for use in adhering tissue sections to both plasticware and glass surfaces for immunohistochemistry. It is intended for use as an adhesive subbing solution for immunohistochemistry (IHC) and routine histologic staining preparations. $30 / 10 mL
DAPI Solution Stains both live and fixed cells. DAPI's blue emission makes it suitable for combined assays where the fluorescence ranges of DAPI and other IHC-employed fluorescent molecules like green-fluorescent fluorescein and GFP, or red-fluorescent stains like Texas Red, are completely distinctive. $15 / 10 mL
DAPI Counterstaining Solution DAPI is a blue fluorescent counterstain solution that is effective in cell staining and quantification of DNA content. Compatible with direct or indirect antibody-based detection method. The applications include immunofluorescence microscopy, in situ hybridization, flow cytometry, or tissue staining. $50 / 50ml
4% Paraformaldehyde 4% Paraformaldehyde solution in PBS is a ready to use solution used for sample preparation for fixing cells for immunohistochemistry (IHC). $50 / 500ml
Antifade Mounting Medium Antifade Mounting Medium is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. Mounted slides can be stored at 4˚C or -20˚C for 2-3 weeks, protected from light. For the brightest signal and lowest background, high definition fluorescent images. Protects against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including FITC, Cy2, Cy3, Rhodamine, Texas Red, Cy5, Cy7, DAPI, AMCA, R6G, Hoechst 33258. $15 / 10ml
*Aqueous Mounting Medium Used to immobilize coverslip over section tissues in IHC and ICC staining. Compatible with chromogens which can dissolve in organic solvents, such as BCIP/NBT, AEC and Fast Red. $30 / 50ml
*3% H2O2 Solution 3% hydrogen peroxide is used to inactivate endogenous peroxidase found in some cell and tissue types that may react with the substrate creating higher levels of background noise. $10 / 50ml
*Citrate Buffer Powder This water-free powder pack allows you to make 2L of Citrate buffer solution with ease. Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. $3 / 2L
Proteinase K 1:100 Concentrated stock solution of Proteinase K in Tris-HCl buffer. Used as assistant pre-treatment reagent in Western blot and IHC assay procedures for general protein digestion in tissue lysates during sample preparation, for eliminating comigrating protein antigens prior to SDS-PAGE, as well as for proteolytic antigen retrieval prior to antibody staining in IHC. $50 / 5ml
*Antigen Retrieval Buffer Ready to use proteolytic enzyme solution used as a pretreatment reagent in IHC for enzymatic digestion of tissues employed in proteolytic induced epitope retrieval (PIER) techniques applied upon formalin-fixed paraffin-embedded (FFPE) tissue sections on slides following deparaffinization and prior to antibody staining. $55 / 50ml
*Normal rabbit serum Dilute the serum 1:10. Use in immunoassays to block non-specific binding, use as an antibody diluent or use as a control. $80 / 100ml
Antibody diluent buffer An optimized solution for the dilution of antibodies that will maintain solubility, activity, and stability. $40 / 12ml
Antibody Dilution Buffer with BSA A universal antibody diluent that can be used to dilute antibodies in all immunoassays. This dilution buffer helps reducing background in these assays. $80 / 100ml
DAB Chromogenic Substrate Kits Kit for staining tissue sections and cells in immunocytochemistry and immunohistochemistry applications. $30 / 1 kit
AEC Substrate Kit AEC is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. $21 / 1kit
*Hematoxylin Counterstain Solution Produces an intense blue nuclear stain. This nuclear counterstain is specifically designed to be used after primary staining during IHC. Suitable for aqueous and non-aqueous tissue section mounts. $15 / 12ml
*Nuclear Fast Red A chromogenic nuclear counterstain solution which produces a good contrast with a variety of substrates. Stained nucleus will have a pink appearance. A popular alternative to hematoxylin and typically stains nuclei in five minutes. $15 / 12ml
SABC Kits Ready to use SABC kits for IHC(P), IHC(F), ICC. Immunohistochemical analyses based on streptavidin-biotin complex has extremely low background. SABC offers high specificity and ease-of-use. $100+ / 1 kit
*PBS Wash Buffer PBS buffer helps to maintain a constant pH. $15 / 500ml
*PBS Powder For making 2 liters PBS buffer, helps to maintain a constant pH. $3 / 2000ml

Did you know Boster can save you 99% on IHC reagents?

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How to Order Services



Turn-Around Time: Typically, we need 5-20 working days to complete a study depending on the project specifications. Sometimes, if the lab-scheduling permits, we can expedite a study by marking it urgent- at no extra charge.

Primary and Secondary Antibodies: Customer needs to provide these antibodies along with test samples. Boster offers an extensive catalog of primary and secondary antibodies which could be included in your service order at listed retail price if they fit your project needs. Currently we also have a promotion which includes a free HRP or biotin secondary antibody to pair with each primary antibody purchase. Alternatively, Boster can purchase these antibodies from vendors like Sigma-Aldrich etc for the customer (an advance payment is required from the researcher).

How to send samples to us: We have received test samples from labs within the USA by FEDEX, UPS, or DHL. One may ship samples on ice, customer should tell us which side should be cut and the purpose they intend to observe. The thickness of tissue should be less than 1/5 inch and there's no limit for size.

How to pay for services: A researcher or institution based in USA may pay for services by Purchase Order or with Credit Card. Services are not offered outside USA.

 

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