SKI Human Over-expression Lysates

Transient overexpression lysate of v-ski sarcoma viral oncogene homolog (avian) (SKI)

Product Info Summary

SKU: LS006497
Size: 100 ug
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of v-ski sarcoma viral oncogene homolog (avian) (SKI)

Product Name

SKI Human Over-expression Lysates

SKU/Catalog Number

LS006497

Size

100 ug

Description

Transient overexpression lysate of v-ski sarcoma viral oncogene homolog (avian) (SKI)

Storage & Handling

Cite This Product

SKI Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006497)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

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LS006497
$720.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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