STAT5A (Phospho-Ser780) Colorimetric Cell-Based ELISA Kit

The STAT5A (Phospho-Ser780) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor STAT5A (Phospho-Ser780) protein expression profile in cells. The kit can be used for measuring the relative amounts of STAT5A (Phospho-Ser780) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT5A (Phospho-Ser780).

Product Info Summary

SKU: EKC2517
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

STAT5A (Phospho-Ser780) Colorimetric Cell-Based ELISA Kit

See all Stat5a products

SKU/Catalog Number

EKC2517

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The STAT5A (Phospho-Ser780) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor STAT5A (Phospho-Ser780) protein expression profile in cells. The kit can be used for measuring the relative amounts of STAT5A (Phospho-Ser780) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on STAT5A (Phospho-Ser780).

Storage & Handling

4°C/6 Months

Cite This Product

STAT5A (Phospho-Ser780) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2517)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC2517 is reactive to STAT5A in Human, Mouse, Rat samples

Application Guarantee

EKC2517 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-STAT5A (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-STAT5A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For STAT5A (Source: Uniprot.Org, NCBI)

Uniprot ID

P42229

Gene ID

6776

Gene Name

STAT5A

Full Name

Signal transducer and activator of transcription 5A

Weight

90.647kDa

Superfamily

transcription factor STAT family

Alternative Names

MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b STAT5A MGF, STAT5 signal transducer and activator of transcription 5A signal transducer and activator of transcription 5A|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on STAT5A, check out the STAT5A Infographic

STAT5A infographic

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In this infographic you will see the following information for STAT5A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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