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SKU AR0143
Pack size 200mL/pack
Applications WB only

Overview

Product Name TBS Blocking Buffer Pack
SKU/Catalog Number AR0143
Pack Size 200mL/pack
Form Dry-blend powder, sufficient to make 200mL of TBS Blocking Buffer
Buffer Contents 50mM Tris, 0.15M NaCl, 5% nonfat powdered milk, pH 7.2
Working concentration 10mL for each membrane of 5cm×8.5cm
Storage Store at 4°C. It is stable at 4°C for one year.
Application WB only
*Our Boster Guarantee covers the use of this product in the above tested applications.
Equivalent Thermofisher (Product No. 37530)
Description Boster’s TBS Blocking Buffer Pack is a pouch of dry-blend powder that is sufficient to make 200mL of TBS Blocking Buffer for Western blot.
Cite This Product TBS Blocking Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0143)

Assay Principle

The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.

Assay Protocol

1. Empty contents of one foil envelope pack into a beaker.

2. Add 200mL of distilled water and stir to dissolve.

TBS Blocking Buffer Pack Images

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TBS Blocking Buffer Pack
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Publications

Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1?-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways
Ischemic postconditioning inhibits apoptosis in an in vitro proximal tubular cell model.
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways.