|Product Name||TBS Blocking Buffer Pack|
|Form||Dry-blend powder, sufficient to make 200mL of TBS Blocking Buffer|
|Buffer Contents||50mM Tris, 0.15M NaCl, 5% nonfat powdered milk, pH 7.2|
|Working concentration||10mL for each membrane of 5cm×8.5cm|
|Storage||Store at 4°C. It is stable at 4°C for one year.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
|Equivalent||Thermofisher (Product No. 37530)|
|Description||Boster’s TBS Blocking Buffer Pack is a pouch of dry-blend powder that is sufficient to make 200mL of TBS Blocking Buffer for Western blot.|
|Cite This Product||TBS Blocking Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0143)|
The purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. However, unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. For best results when developing a new immunoassay, test several different blocking agents for the highest signal-to-noise ratio in the assay. There is no single blocking agent that is ideal for every occasion because many factors can influence nonspecific binding, including various protein interactions unique to a specific assay system.
1. Empty contents of one foil envelope pack into a beaker.
2. Add 200mL of distilled water and stir to dissolve.
TBS Blocking Buffer Pack Images
Click the images to enlarge.