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SKUAR0031
Pack size 2000ml/pack
Form Powder
Applications ELISA: pH stabilizing
Western blots: wash buffer, antibody diluent
IHC: background noise reduction
Price: $3.00
Quantity:

Overview

Product Name Tris Buffered Saline (TBS) Powder
Synonyms TBS powder, Tris buffered saline powder, TBS buffer powder form
Description TBS Buffer, For dilution of active biological reagents, as well as cleaning and antigen retrieval in the IHC. TBS powder when reconstituted in distilled water yields 2L; pH 7.2-7.6
Application pH stabilizing solution in immunoassays; wash buffer and antibody diluent in Western blotting; Background noise reduction in IHC
Pack Size 2000ml/pack

Properties

Composition sodium chloride, Tris, Tris-hydrochloride
Content 17g NaCl, 0.6g Tris, 2.4g Tris-HCl
Form Supplied Powder
Physical State White crystalline powder
Pack Size 1 pouch for 2000ml final solution
Volume of Final Solution 2000 ml
Molar Concentration of Final Solution 20mM Tris-HCl, 150mM NaCl
pH 7.2 - 7.6
Enzymatic Activity DNase-, RNase-, Protease- free
Storage Store at room temperature (RT) for one year

Chemical Information

Molecular Formula sodium chloride NaCl
Tris C4H11NO3
H2NC(CH2OH)3
Tris-hydrochloride C4H12ClNO3
H2NC(CH2OH)3·HCl
CAS sodium chloride 7647-14-5
Tris 77-86-1
Tris-hydrochloride 1185-53-1
Molecular Weight sodium chloride 58.44
Tris 121.14
Tris-hydrochloride 157.6

Usage and Handling

TBS is safe and non-toxic, requiring no special handling considerations.

Dissolve 1 pouch of TBS powder in distilled water to make a total volume of 2000 ml ready to use buffer solution.

Background

Tris buffered saline (TBS) is an isotonic pH stabilizing buffer solution commonly used in immunoassay procedures providing optimal conditions for antibody-antigen binding interactions. It is utilized in IHC as substance diluent for washes between sequential immunoreactions to remove unbound immunological agents, and for incubation mixtures for labeled antibodies and other immunoreagents to reduce unspecific background noise. In Western blotting TBS buffer is a main component in the composition of the buffers used in the washing, blocking and antibody diluting procedures.

Tris Buffered Saline (TBS) Powder Images

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Tris Buffered Saline (TBS) Powder
Structural formula of Tris plus main ions added;
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Publications

Jian Cx, Yang Mz, Li P, Xiong J, Zhang Zj, Li Cj, Chen A, Hu Cm, Zhou Jx, Li Sh. Cancer Invest. 2012 Dec;30(10):748-56. Doi: 10.3109/07357907.2012.734355. Epub 2012 Oct 22. Ectopically Expressed Ibp Promotes Cell Proliferation In Oral Squamous Cel...