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Mettl23 Detection in Cell Lines

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41142
Application Western Blot
Sample Human cell lines
Primary Incubation 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
Blocking Agent 5% BSA in TBS
Secondary Incubation Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
Detection Licor Odyssey IR Scanner
Additional Notes The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery).
Results Summary The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.

"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."

Beautiful Imaging of Zebrafish Eosinophils

Excellent, submitted by on

Source: Biocompare.com

SKU DZ41383
Application Immunofluorescence
Sample Zebrafish WKM, IPEX and FFPE tissue sections
Primary Incubation Overnight
Blocking Agent 3% goat serum in PBS
Secondary Incubation 1 hour
Tertiary Incubation None
Detection Fluorescence microscopy
DOI or PMID # https://doi.org/10.1101/2024.04.29.591640
Results Summary Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF.

"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."

"Works well for immunofluorescence"

Excellent, submitted by on

Source: Customer Feedback Submission

"Works well for immunofluorescence"

--Jason Tennessen

SKU DZ41223
Application IF
Sample Drosophila larval fat body

"We’ve verified the specificity of this custom antibody that Boster generated for us. The antibody "Anti-Fruit Fly Gpdh1 Antibody (DZ41223)” works well for immunofluorescence. A 1:100 dilution of this antibody stains fat body tissues of wild-type larvae but not Gpdh1 mutant. I’m happy to advertise this antibody to the fly community."

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
SKU DZ41152
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."

Good Zebrafish Mef2 Antibody from Boster Bio

--Jingli Cao, Assistant Professor, Weill Cornell Medical College

Excellent, submitted by on
SKU DZ01398-1
Application Immunofluorescence
Sample Zebrafish heart
Detection Confocal Microscopy

"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU M00593
Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

A Good Drosophila Me31B Antibody for WB

--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33938-1
Application Western Blot
Sample Insect, D. melanogaster
Detection ECL

"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."

Good Zebrafish CDHR1a Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ07988
Application Immunofluorescence
Sample Zebrafish embryos, 5 days old
Detection Confocal Microscopy

"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"

Good Zebrafish-Specific Siah1 Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ02095
Application Immunofluorescence
Sample Zebrafish embryos 5dpf
Detection Confocal Microscopy

"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"