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Good Zebrafish Mef2 Antibody from Boster Bio

--Jingli Cao, Assistant Professor, Weill Cornell Medical College

Excellent, submitted by on
SKU DZ01398-1
Application Immunofluorescence
Sample Zebrafish heart
Detection Confocal Microscopy

"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."

Immunohistochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by on
SKU M01513-4
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Lung
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU M00593
Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on
PB9561 Immunohistochemistry

The antibody worked perfectly, Great sensitivity

SKU PB9561
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent, submitted by on
SKU M00063-2
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Oesophagus
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Great PKC antibody for IHC

Excellent, submitted by on
We used Anti-PKC Alpha Picoband™ Antibody (SKU: PB9318) for IHC experiment from mouse intestine tissues. Clear positive staining with the right subcellular locations. Super easy to determine the dilution ratio! Great quality.

A Good Drosophila Me31B Antibody for WB

--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33938-1
Application Western Blot
Sample Insect, D. melanogaster
Detection ECL

"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

SKU DZ33980
Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."

A Good PKC Alpha Antibody for IP and WB

--Ramaz Geguchadze, University of New England, Biomedical Sciences, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

SKU PB9318
Application Western Blot
Sample Dorsal root ganglia (neuronal cells)
Detection CyDye

"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."

Good Zebrafish CDHR1a Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

SKU DZ07988
Application Immunofluorescence
Sample Zebrafish embryos, 5 days old
Detection Confocal Microscopy

"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"