Customer Testimonials

Source: Biocompare.com

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")

Source: Biocompare.com

"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."

Source: Biocompare.com

"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")

Source: Biocompare.com

"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")

Source: Biocompare.com

"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")

Source: Biocompare.com

"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")

Source: Biocompare.com

"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")

Source: Biocompare.com

"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")

Source: Biocompare.com

"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")

Source: Biocompare.com

"Our research focuses on in vitro modeling of organ fibrosis and drug discovery. The protocol for this product was easy to perform. However, there was some amount of variability with the replicates. Was effective in detecting A2M levels at pg levels. Reasonable sensitivity and reproducibility. Easy to use." (Customer’s Tip: "Follow protocol to the T.")