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The antibody shows a clear target band at the correct molecular weight and exhibits strong affinity for phosphorylated EIF2A protein in pig samples.

Excellent, submitted by on
SKU P04387
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Phosphorylation of eIF2α is one of the most important switches controlling intracellular protein synthesis. When cells encounter conditions such as oxidative stress or endoplasmic reticulum stress, phosphorylated eIF2α rapidly suppresses the majority of protein synthesis to help the cell cope with the crisis. The purpose of this experiment is to determine the expression levels of phosphorylated EIF2A protein in different intestinal segments infected with classical swine fever virus.

The target band of this antibody is clear and at the correct position, and it shows strong affinity for porcine GRP78 protein.

Excellent, submitted by on
SKU PA1815
Application Western Blot
Sample Porcine intestinal tissue
Sample Processing Description Intestinal tissues from different segments of pigs infected with swine fever.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-GRP78 BiP/HSPA5 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary When cells experience stress such as hypoxia, nutrient deprivation, or calcium imbalance, large amounts of unfolded proteins accumulate in the endoplasmic reticulum. GRP78 senses this stress and undergoes dissociation upon upregulation, activating a series of signaling pathways to reduce new protein synthesis and increase the production of molecular chaperones (including GRP78 itself) to relieve the stress. The aim of the experiment is to determine the expression levels of GRP78 in different intestinal segments of pigs infected with swine fever.

The antibody shows a clear target band, correct positioning, minimal background, and overall good results.

Excellent, submitted by on
SKU PB9253
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody HIF-1-alpha/HIF1A Antibody Picoband®
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the HIF1A protein levels showed clear differences among the groups: the blank group had the lowest level, the model group the highest, and in the drug-treated groups, the levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

The antibody shows a clear target band, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, total MTOR protein levels were similar across all groups. However, phosphorylated MTOR levels showed significant changes: the blank group was the lowest, the model group the highest, and in the drug-treated groups, phosphorylation decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00024-4
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-AKT1,2,3 Antibody Picoband®
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Under equal loading conditions, the total AKT protein levels were similar across all groups, while the amount of phosphorylated AKT1 showed significant changes. The blank group had the lowest level, the model group had the highest, and in the drug-treated groups, the levels decreased progressively from low to medium to high dose. The positive control drug group was close to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the total MTOR protein levels were similar across all groups. However, the levels of phosphorylated MTOR showed significant changes: the blank group had the lowest, the model group the highest, and in the drug-treated groups, the phosphorylation levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

Boster antibodies have good quality and high cost-effectiveness, and will be my preferred brand in the future.

Excellent, submitted by on
SKU A00024-2
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the total AKT protein levels were similar across all groups. However, the levels of phosphorylated AKT1 showed significant changes: the blank group had the lowest, the model group the highest, and in the drug-treated groups, phosphorylation levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

Our lab will continue to use this antibody, and we will recommend it to other researchers at the university working on similar studies.

Excellent, submitted by on
SKU A31732-2
Application Immunofluorescence
Sample Normal rat liver and alcoholic rat liver
Sample Processing Description The samples consist of myocardium from normal SD rats and myocardium from SD rats subjected to an alcoholic liver disease model. The alcoholic liver disease model was established by gavaging 50% ethanol twice daily, while allowing the rats to freely consume alcoholic beverages, for a total of 14 weeks.
Other Reagents Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody Anti-PTRF/CAVIN1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:500, 45 minutes in room temperature
Detection Imaging system:Leica DM2500
Results Summary PTRF/CAVIN1 is a multifunctional protein that shuttles between the plasma membrane and the nucleus, assisting in the recruitment of repair proteins when the plasma membrane is damaged. The results of this IF experiment indicate that alcohol gavage induces myocardial injury and leads to increased expression of PTRF.

This antibody has good specificity, clearly labels endothelial cells, exhibits low background, and produces very clean and beautiful results.

Excellent, submitted by on
SKU A01513-3
Application Immunofluorescence
Sample Normal rat liver and alcoholic rat liver
Sample Processing Description The samples consist of livers from normal SD rats and livers from SD rats with an alcoholic liver disease (ALD) model. The ALD model was established by gavaging the rats twice daily with 50% alcohol, while allowing them free access to alcoholic beverages, for a continuous period of 14 weeks.
Other Reagents Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody Anti-CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)
Secondary Incubation 1:500, 45 minutes in room temperature
Detection Imaging system:Leica DM2500
Results Summary CD31 is a marker of vascular endothelial cells. In normal liver, it is expressed in the endothelium of blood vessels, while liver sinusoidal endothelial cells (LSECs) show minimal expression. However, in alcoholic liver disease, the phenotype of LSECs changes and they begin to express CD31, leading to an overall increase in CD31 expression in the liver. This has also been confirmed by experimental results.

Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.