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SKU AR1149
Pack size 1L/pack
Applications Transfer buffer for Tris-glycine-sds gel

Overview

Product Name Tris-Glycine SDS Buffer Pack
SKU/Catalog Number AR1149
Pack Size 1L/pack
Form Dry-blend powder, sufficient to make 1L of standard transfer buffer
Buffer Contents 25 mM Tris, 192 mM glycine, 0.1%SDS, pH 8.3
Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated areaat room temperature. It is stable for one year.
Buffer Type Transfer Buffers
Gel Compatibility SDS-PAGE Gels
Equivalent Thermofisher (Product No. 28380, 28363); Millipore Sigma (Product No. 93321, 93015)
Description Boster’s Tris-Glycine SDS Buffer Pack is a pouch of dry-blend powder that is sufficient to make 1L of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane.
Cite This Product Tris-Glycine SDS Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1149)
Application Transfer buffer for Tris-glycine SDS gel
*Our Boster Guarantee covers the use of this product in the above tested applications.

Assay Principle

Tris-Glycine SDS Buffer contains the denaturing agent sodium dodecyl sulphate (SDS). Protein electrophoresis under denaturing conditions (SDS-PAGE) involves separating proteins based on their size. By treating the sample under denaturing and reducing conditions with SDS, proteins unfold and become coated with SDS detergent molecules.

Assay Protocol

1. Empty contents of one foil envelope pack into a beaker.

2. Add 800mL of distilled water and stir to dissolve.

3. Add 200mL dried methyl alcohol, mix thoroughly.

Tris-Glycine SDS Buffer Pack Images

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Tris-Glycine SDS Buffer Pack
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