Tris-Glycine SDS Running Buffer (10X)

Boster’s Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). It separates proteins in their denatured state on Tris-Glycine gels.

Product Info Summary

SKU AR1146-10
Pack size 500ml (for 25 assays)
Form Liquid
Applications Running buffer for Tris-glycine gel electrophoresis

Overview

Product Name TTris-Glycine SDS Running Buffer (10X)
SKU/Catalog Number AR1146-10
Pack Size 500ml (for 25 assays)
Form Liquid
Buffer Contents 25mM Tris,192mM glycine, 0.1%SDS, pH 8.6
Storage Store at room temperature or at 4˚C. It is stable for one year.
Application Running buffer for Tris-glycine gel electrophoresis
*Our Boster Guarantee covers the use of this product in the above tested applications.
Buffer Type Running buffer
Equivalent Thermofisher (Product No. LC2675, LC26754, LC26755);
Millipore Sigma (Product No. T7777)
Cite This Product Tris-Glycine SDS Running Buffer (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1146-10)

Description

Boster’s Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). It separates proteins in their denatured state on Tris-Glycine gels.

Buffer Preparation

Add 100 mL of Tris-Glycine SDS Running Buffer (10X) to 900 mL of ultrapure water or deionized water and mix well to prepare 1X SDS Running Buffer. Dilute Tris-Glycine SDS Running Buffer (10X) to a 1X solution using ultrapure water or deionized water based on needs.

Notes:
1. Discard prepared 1X SDS Running Buffer after two weeks.
2. The recycled running buffer can be reused to fill outer buffer chamber of electrophoresis cell for 1-2 times. Freshly prepared running buffer is recommended to be used in order to have an optimal electrophoresis result.

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