Show Order Info
SKU AR1175
Pack size 1kit (for 1000 assays)
Form Supplied Solution
Applications Detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability and proliferative assays

List of Components

DescriptionQuantityVolumeContentsCatalog Number
Trypan Blue Solution (2X)110mL0.08% Trypan BlueAR1175-1
Cell Diluent Buffer1100mL0.9% Sodium ChlorideAR1175-2

Overview

Product Name Trypan Blue Assay Kit
SKU/Catalog Number AR1175
Pack Size 1kit (for 1000 assays)
Form Supplied Solution
Appearance Blue
Storage Upon receipt store at 4°C for one year.
For Use With (Equipment) Microplate Reader
Sub-Cellular Localization Cytoplasm & Cytosol
Detection Method Colorimetric
Equivalent Thermofisher (Product No. 15250061, T10282); Millipore Sigma (Product No. T8154)
Description Boster’s Trypan Blue Assay Kit provides reagents for a vital cell stain used to assess cell viability using the dye exclusion test. The staining process can be finished in 3-5 minutes.
Application Detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability and proliferative assays
Cite This Product Trypan Blue Assay Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1175)

Chemical structures

Molecular formula: C34H24N6Na4O14S4

Molecular weight: 960.81

CAS Number: 72-57-1

Background

Trypan Blue is a vital cell stain used to assess cell viability using the dye exclusion test. Live cells with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue colour under a microscope. And Trypan blue is commonly used for cell counting. The method cannot distinguish between necrotic and apoptotic cells.

Protocol

1. Cell Collection

a. Trypsinize adherent cells or digest cells with EDTA; For suspending cells, collect directly.

b. Centrifuge at ~1000-2000 x g for 1 min, remove supernatant.

c. Add some cell diluent buffer into the sediment and resuspend pellet.


2. Staining

a. Add 90 μl cell suspension into EP tube.

b. Add 10 μl trypan blue solution (2X) in the EP tube and mix thoroughly.

c. Place in a CO2 incubator for 3-5 min.
Note: Do not incubate too long.


3. Cell Viability Analysis

Place the cell suspension with trypan blue solution onto blood cell counting plate. Calculate the total number of cells and the number of cells dyed to blue respectively.

Cell Viability = (total number of cells - number of blue cells) / total number of cells X 100%

Note: Please wear gloves to operate.

Trypan Blue Assay Kit Images

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Trypan Blue Assay Kit
Chemical structure
Trypan Blue Assay Kit
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Guo XH, Ni J, Xue JL, Wang X. J Zhejiang Univ Sci B. 2017 Dec.;18(12):1031-1045. doi: 10.1631/jzus.B1600542. Phyllanthus emblica Linn. fruit extract potentiates the anticancer efficacy of mitomycin C and cisplatin and reduces their genotoxicity to...