|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Rb2 p130 Antibody|
|Description||Rabbit IgG polyclonal antibody for Retinoblastoma-like protein 2(RBL2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Rb2 p130 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1895)|
|Replacement Item||This antibody may replace the following items: sc-16299|sc-16299-R|sc-16300|sc-16300-R|sc-16301|sc-16301-R|sc-169028|sc-20678|sc-22157|sc-22157-R|sc-31338|sc-31339|sc-317|sc-317-G|sc-317-X|sc-365163|sc-365163-X|sc-374521|sc-53641|sc-56176|sc-9963 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Rb2 p130(1120-1139aa ENHSALLRRLQDVANDRGSH), identical to the related rat sequence and different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Retinoblastoma-like protein 2|
|Molecular Weight||128367 MW|
|Protein Function||Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.|
|Sequence Similarities||Belongs to the retinoblastoma protein (RB) family.|
|Alternative Names||Retinoblastoma-like protein 2;130 kDa retinoblastoma-associated protein;p130;Retinoblastoma-related protein 2;RBR-2;pRb2;RBL2;RB2;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors| epigenetics and nuclear signaling| cancer|oncoproteins/suppressors|tumor suppressors|rb family||
Background for Retinoblastoma-like protein 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Rb2 p130 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Rb2 p130 Antibody Images
Click the images to enlarge.
Lane 1: Rat Testis Tissue Lysate
Lane 2: NIH313 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,