Human TNF Alpha ELISA Kit PicoKine™

Catalog number	Description	Quantity
EK0525-CAP	96-well plate precoated with anti-Human TNF-alpha antibody	1
EK0525-ST	lyophilized recombinant Human TNF-alpha standard	10ng/tube
EK0525-DA	biotinylated anti-Human TNF-alpha antibody	130ul
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul
AR1106-1	sample diluent buffer	30ml
AR1106-2	antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4
-	Wash Buffer	Powder pack for 1000ml

• Microplate reader in standard size.
• Automated plate washer.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

This data is generated from a recent batch of EK0525 Human TNF Alpha ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.091	0.158	0.197	0.320	0.548	0.897	1.576	2.147

We measured random samples of Human TNF Alpha ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	82	201	14	75	186
Standard deviation	0.76	5.49	12.66	0.75	5.55	11.9
CV(%)	4.8%	6.7%	7.4%	5.4%	7.4%	6.4%

We measured the performance consistency of Human TNF Alpha ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	16	15	15	16	15	0.5	3.3%
Sample 2	82	91	75	89	84	6.29	7.4%
Sample 3	201	204	215	219	209	7.46	3.5%

*number of samples for each test n=16.