Human TNF Alpha PicoKine™ ELISA Kit

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SKU EK0525
Size 96wells/kit, with removable strips.
Reactivity Human
Sample Type cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)

Overview

Product Name Human TNF Alpha PicoKine™ ELISA Kit
SKU/Catalog Number EK0525
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF alpha. 96wells/kit, with removable strips.
Cite This Product Human TNF Alpha PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0525)
Sample Type cell culture supernates, serum and plasma(heparin, EDTA, citrate).. Anticoagulant(s): heparin, EDTA or citrate
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <1pg/ml
Assay Range 15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)
Immunogen Expression system for standard: E.coli, Immunogen sequence: V77-L233
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog numberDescriptionQuantity
EK0525-CAP96-well plate precoated with anti-Human TNF antibody1
EK0525-STlyophilized recombinant Human TNF standard10ng/tube
EK0525-DAbiotinylated anti-Human TNF antibody130ul
AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul
AR1106-1sample diluent buffer30ml
AR1106-2antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Human TNF Alpha PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-25min)

Concentration(pg/ml)015.631.262.51252505001000
O.D.0.1230.2010.2500.3890.6791.0941.7852.257

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)16822011475186
Standard deviation0.765.4912.660.755.5511.9
CV(%)4.8%6.7%7.4%5.4%7.4%6.4%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116151516150.53.3%
Sample 282917589846.297.4%
Sample 32012042152192097.463.5%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01375
Gene Name TNF
Protein Name Tumor necrosis factor
Alternative Names Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;TNF;TNFA, TNFSF2;
Subcellular Localization Cell membrane ; Single-pass type II membrane protein .
Molecular Weight 25644 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918). .
Research Areas Atherosclerosis, Cancer, Cardiovascular, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Metabolism, Signal Transduction, Tnf Superfamily, Vascular Inflammation

*You can search these to find other products in these research areas.
Background Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

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Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...
PubMed ID26055622
Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.
PubMed ID25071347
Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...
PubMed ID21246393
Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.
PubMed ID21134421
Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.
PubMed ID22251871
Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.
PubMed ID23851615
Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...
PubMed ID21234610
Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...
PubMed ID24530793
Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...
PubMed ID20138164
Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.
PubMed ID25329073
Dai Jp, Zhao Xf, Zeng J, Wan Qy, Yang Jc, Li Wz, Chen Xx, Wang Gf, Li Ks. Plos One. 2013 Apr 16;8(4):E61026. Doi: 10.1371/Journal.Pone.0061026. Print 2013. Drug Screening For Autophagy Inhibitors Based On The Dissociation Of Beclin1-Bcl2 Complex U...
PubMed ID23613775
Ping C, Yongping Z, Minmin Q, Weiyan Y, Yaozong Y. Dig Dis Sci. 2010 Jun;55(6):1599-609. Doi: 10.1007/S10620-009-0909-Y. Epub 2009 Aug 13. Activated Protein C Improves The Severity Of Severe Acute Pancreatitis Via Up-Regulating The Expressions Of ...
PubMed ID19680809
Zhang H, Yin G, Jiang H, Zhang C. J Int Med Res. 2013 Aug;41(4):1179-86. Doi: 10.1177/0300060513488503. Epub 2013 Jun 14. High-Dose N-Acetylcysteine Decreases Silica-Induced Lung Fibrosis In The Rat.
PubMed ID23771710
Li Mc, Yang G, Zhou Xd, Tselluyko S, Perelman Jm. Int J Mol Med. 2014 Jan;33(1):83-90. Doi: 10.3892/Ijmm.2013.1535. Epub 2013 Oct 23. The Pathophysiological Mechanisms Underlying Mucus Hypersecretion Induced By Cold Temperatures In Cigarette Smoke...
PubMed ID24154796
Ren Dl, Li Yj, Hu Bb, Wang H, Hu B. J Pineal Res. 2015 May;58(4):452-60. Doi: 10.1111/Jpi.12230. Epub 2015 Mar 26. Melatonin Regulates The Rhythmic Migration Of Neutrophils In Live Zebrafish.
PubMed ID25763660
Liu L, Li C, Xiang J, Dong W, Cao Z. J Periodontal Res. 2013 Oct;48(5):615-22. Doi: 10.1111/Jre.12047. Epub 2013 Feb 27. Over-Expression And Potential Role Of Cyclophilin A In Human Periodontitis.
PubMed ID23441725
Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...
PubMed ID22863994
Sun Y, Zhao Y, Yao J, Zhao L, Wu Z, Wang Y, Pan D, Miao H, Guo Q, Lu N. Biochem Pharmacol. 2015 Mar 15;94(2):142-54. Doi: 10.1016/J.Bcp.2015.02.002. Epub 2015 Feb 10. Wogonoside Protects Against Dextran Sulfate Sodium-Induced Experimental Colitis ...
PubMed ID25677765
Huang Q, Xue J, Zou R, Cai L, Chen J, Sun L, Dai Z, Yang F, Xu Y. Exp Ther Med. 2014 Nov;8(5):1648-1654. Epub 2014 Sep 11. Nr4A1 Is Associated With Chronic Low-Grade Inflammation In Patients With Type 2 Diabetes.
PubMed ID25289075
Sun L, Deng H, He L, Hu X, Huang Q, Xue J, Chen J, Shi X, Xu Y. J Diabetes Complications. 2015 May-Jun;29(4):589-94. Doi: 10.1016/J.Jdiacomp.2014.12.018. Epub 2014 Dec 31. The Relationship Between Nr2E1 And Subclinical Inflammation In Newly Diagno...
PubMed ID25813674
Hu Mm, Yang Q, Zhang J, Liu Sm, Zhang Y, Lin H, Huang Zf, Wang Yy, Zhang Xd, Zhong B, Shu Hb. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1509-14. Doi: 10.1073/Pnas.1318227111. Epub 2014 Jan 13. Trim38 Inhibits Tnf??- And Il-1??-Triggered Nf-??b ...
PubMed ID24434549
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
PubMed ID25674205
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
PubMed ID24137221
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
PubMed ID22509346
Meng H, Shen Y, Shen J, Zhou F, Shen S, Das Un. Lipids Health Dis. 2013 Oct 29;12:160. Doi: 10.1186/1476-511X-12-160. Effect Of N-3 And N-6 Unsaturated Fatty Acids On Prostate Cancer (Pc-3) And Prostate Epithelial (Rwpe-1) Cells In Vitro.
PubMed ID24168453
Wang M, Zheng Z, Meng J, Wang H, He M, Zhang F, Liu Y, Hu B, He Z, Hu Q, Wang H. Biomaterials. 2015 Sep;64:78-87. Doi: 10.1016/J.Biomaterials.2015.06.030. Epub 2015 Jun 20. In Vivo Study Of Immunogenicity And Kinetic Characteristics Of A Quantum D...
PubMed ID26117660
Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...
PubMed ID25840281
Wang Y, Zhang Zz, Wu Y, Zhan J, He Xh, Wang Yl. Exp Ther Med. 2013 Jan;5(1):315-319. Epub 2012 Oct 25. Honokiol Protects Rat Hearts Against Myocardial Ischemia Reperfusion Injury By Reducing Oxidative Stress And Inflammation.
PubMed ID23251290
Zhan Y, Wang Z, Yang P, Wang T, Xia L, Zhou M, Wang Y, Wang S, Hua Z, Zhang J. Cell Death Dis. 2014 Jan 9;5:E985. Doi: 10.1038/Cddis.2013.516. Adenosine 5'-Monophosphate Ameliorates D-Galactosamine/Lipopolysaccharide-Induced Liver Injury Through A...
PubMed ID24407238
Ma L, Wang G, Chen Z, Li Z, Yao J, Zhao H, Wang S, Ma Z, Chang H, Tian X. Scientificworldjournal. 2014 Mar 16;2014:387640. Doi: 10.1155/2014/387640. Ecollection 2014. Modulating The P66Shc Signaling Pathway With Protocatechuic Acid Protects The In...
PubMed ID24757420
Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.
PubMed ID23123425
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Transl Res. 2010 Dec;156(6):326-34. Doi: 10.1016/J.Trsl.2010.09.001. Epub 2010 Oct 1. Nicotine Suppresses Inflammatory Factors In Hbe16 Airway Epithelial Cells After Exposure To Cigarette Smoke Extract And Li...
PubMed ID21078494
Dong Gh, Gong Jp, Li Jz, Luo Yh, Li Zd, Li Pz, He K. Inflammation. 2013 Oct;36(5):1087-93. Doi: 10.1007/S10753-013-9641-Z. Association Between Gene Polymorphisms Of Irak-M And The Susceptibility Of Sepsis.
PubMed ID23588345
Li X, Peng F, Xie C, Wu W, Han X, Chen L. Int Immunopharmacol. 2013 Dec;17(4):1125-33. Doi: 10.1016/J.Intimp.2013.10.001. Epub 2013 Oct 14. (E)-3-(3,4-Dimethoxyphenyl)-1-(5-Hydroxy-2,2-Dimethyl-2H-Chromen-6-Yl)Prop-2-En-1-One Ameliorates The Colla...
PubMed ID24135236
Guo R, Wu K, Chen J, Mo L, Hua X, Zheng D, Chen P, Chen G, Xu W, Feng J. Cell Physiol Biochem. 2013;32(6):1668-80. Doi: 10.1159/000356602. Epub 2013 Dec 13. Exogenous Hydrogen Sulfide Protects Against Doxorubicin-Induced Inflammation And Cytotoxic...
PubMed ID24356372
Wang G, Chen Z, Zhang F, Jing H, Xu W, Ning S, Li Z, Liu K, Yao J, Tian X. Apoptosis. 2014 Sep;19(9):1342-53. Doi: 10.1007/S10495-014-1008-X. Blockade Of Pkc?? Protects Against Remote Organ Injury Induced By Intestinal Ischemia And Reperfusion Via...
PubMed ID24930012
Wei Z, Yang Y, Xie C, Li C, Wang G, Ma L, Xiang M, Sun J, Wei Y, Chen L. Fitoterapia. 2014 Sep;97:172-83. Doi: 10.1016/J.Fitote.2014.06.002. Epub 2014 Jun 9. Synthesis And Biological Evaluation Of Pyranoisoflavone Derivatives As Anti-Inflammatory ...
PubMed ID24924289
Xu W, Lu Y, Yao J, Li Z, Chen Z, Wang G, Jing H, Zhang X, Li M, Peng J, Tian X. Shock. 2014 Nov;42(5):440-7. Doi: 10.1097/Shk.0000000000000225. Novel Role Of Resveratrol: Suppression Of High-Mobility Group Protein Box 1 Nucleocytoplasmic Transloca...
PubMed ID25004063
Sun Ln, Yang Zy, Lv Ss, Liu Xc, Guan Gj, Liu G. Int Immunopharmacol. 2014 Nov;23(1):236-46. Doi: 10.1016/J.Intimp.2014.08.023. Epub 2014 Sep 4. Curcumin Prevents Diabetic Nephropathy Against Inflammatory Response Via Reversing Caveolin-1 Tyr14 Pho...
PubMed ID25196431
Zhang F, Li Zl, Xu Xm, Hu Y, Yao Jh, Xu W, Jing Hr, Wang S, Ning Sl, Tian Xf. Mol Med Rep. 2015 Jan;11(1):269-76. Doi: 10.3892/Mmr.2014.2679. Epub 2014 Oct 16. Protective Effects Of Icariin-Mediated Sirt1/Foxo3 Signaling Pathway On Intestinal Isch...
PubMed ID25323330
Ni J, Wang X, Chen S, Liu H, Wang Y, Xu X, Cheng J, Jia J, Zhen X. Brain Behav Immun. 2015 Oct;49:75-85. Doi: 10.1016/J.Bbi.2015.04.014. Epub 2015 Apr 29. Microrna Let-7C-5P Protects Against Cerebral Ischemia Injury Via Mechanisms Involving The In...
PubMed ID25934573
Jin Y, Zhang Y, Wan C, Wang H, Hou L, Chang J, Fan K, Xie X. Evid Based Complement Alternat Med. 2013;2013:194976. Doi: 10.1155/2013/194976. Epub 2013 Sep 18. Immunomodulatory Activity And Protective Effects Of Polysaccharide From Eupatorium Adeno...
PubMed ID24159339
Lu Dh, Guo Xy, Qin Sy, Luo W, Huang Xl, Chen M, Wang Jx, Ma Sj, Yang Xw, Jiang Hx. World J Gastroenterol. 2015 Feb 7;21(5):1531-45. Doi: 10.3748/Wjg.V21.I5.1531. Interleukin-22 Ameliorates Liver Fibrogenesis By Attenuating Hepatic Stellate Cell Ac...
PubMed ID25663772
Xie Wl, Shi Q, Zhang J, Zhang By, Gong Hs, Guo Y, Wang Sb, Xu Y, Wang K, Chen C, Liu Y, Dong Xp. J Mol Neurosci. 2013 Nov;51(3):919-32. Doi: 10.1007/S12031-013-0002-Z. Epub 2013 Mar 24. Abnormal Activation Of Microglia Accompanied With Disrupted C...
PubMed ID23526370
Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, Shi G. Plos One. 2014 Apr 21;9(4):E95931. Doi: 10.1371/Journal.Pone.0095931. Ecollection 2014. Anti-Inflammatory Activity Of N-Butanol Extract From Ipomoea Stolonifera In Vivo And In Vitro.
PubMed ID24752203
Fan Z, Jing H, Yao J, Li Y, Hu X, Shao H, Shen G, Pan J, Luo F, Tian X. Oxid Med Cell Longev. 2014;2014:191624. Doi: 10.1155/2014/191624. Epub 2014 Aug 20. The Protective Effects Of Curcumin On Experimental Acute Liver Lesion Induced By Intestinal...
PubMed ID25215173
Zheng Ls, Kaneko N, Sawamoto K. Front Cell Neurosci. 2015 Jan 28;9:5. Doi: 10.3389/Fncel.2015.00005. Ecollection 2015. Minocycline Treatment Ameliorates Interferon-Alpha- Induced Neurogenic Defects And Depression-Like Behaviors In Mice.
PubMed ID25674053
Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.
PubMed ID23786840
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
PubMed ID24955162
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...
PubMed ID23746956
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.
PubMed ID24710706
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...
PubMed ID25725585
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.
PubMed ID25383758
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
PubMed ID26022197
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
PubMed ID25626689
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.
PubMed ID22095388
Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.
PubMed ID25880347
Li P, Wang B, Sun F, Li Y, Li Q, Lang H, Zhao Z, Gao P, Zhao Y, Shang Q, Liu D, Zhu Z. Sci Rep. 2015 May 28;5:10229. Doi: 10.1038/Srep10229. Mitochondrial Respiratory Dysfunctions Of Blood Mononuclear Cells Link With Cardiac Disturbance In Patient...
PubMed ID26018291
Feng X, Lv C, Wang F, Gan K, Zhang M, Tan W. Clin Dev Immunol. 2013;2013:160123. Doi: 10.1155/2013/160123. Epub 2013 Nov 18. Modulatory Effect Of 1,25-Dihydroxyvitamin D 3 On Il1 ?? -Induced Rankl, Opg, Tnf ?? , And Il-6 Expression In Human Rheuma...
PubMed ID24348674
Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.
PubMed ID24738018
Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And...
PubMed ID25953665
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...
PubMed ID24368037
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
PubMed ID25744647
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.
PubMed ID24571896
Tong Gq, Zhang Zh, Zhao Y, Liu Jj, Han Jb. Arch Environ Contam Toxicol. 2015 Jan;68(1):31-7. Doi: 10.1007/S00244-014-0077-8. Epub 2014 Sep 7. Traffic-Related Pm2.5 Induces Cytosolic [Ca?????] Increase Regulated By Orai1, Alters The Can-Nfat Signal...
PubMed ID25194241
Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...
PubMed ID24593676
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????
PubMed ID25428340
Han X, Wang T, Zhang J, Liu X, Li Z, Wang G, Song Q, Pang D, Ouyang H, Tang X. Biol Open. 2015 Apr 2;4(5):661-5. Doi: 10.1242/Bio.201410900. Apolipoprotein Ciii Regulates Lipoprotein-Associated Phospholipase A2 Expression Via The Mapk And Nf??b Pa...
PubMed ID25836672
Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis.
PubMed ID25044235
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...
PubMed ID23642811
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...
PubMed ID24685824
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.
PubMed ID23382627

Customer Q&As

Q: How many samples can I test on one ELISA plate?
A: On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate.
Q: The volumes of my samples are small, would it be possible to use 50µl/sample?
A: There are two solutions for insufficient sample volume.
1. Dilute the samples with the provided sample diluent buffer into 100ul.
2. If 50ul of sample is added into a well, add 50ul of standard solution.
Q: Will this kit work on tissue samples?
A: For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
Q: Is it possible to dilute 10X TBS stock that is 250mM Tris Base/Tris-Hcl, 27 mM KCl, and 1.37M NaCl to be used as the wash buffer?
A: You can dilute the 10X TBS stock to 1XTBS and test the pH value of the 1xTBS.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Q: Would your kits work normally with samples from Wistar Rat?
A: Our kits would work normally with samples from Wistar Rat.
Q: How can I store the dissolved wash buffer for a longer period of time?
A: You can prepare a concentrated wash buffer (25X) which can be stored at 4°C for 1-3 months.
Q: Does EK0525, EK0392, EK0373 react with BSA?
A: EK0525, EK0392, EK0373 does not react with BSA.
Q: For EK0525, EK0392, EK0373, do these kits have binding for the forms of cytokine other than just the active form?
A: EK0525, EK0392, EK0373 are able to detect total forms of TNF-Alpha, IL-1Beta, and IFN-Gamma.
Q: Does the TMB color developing agent contain H2O2?
A: Yes, the TMB color developing agent contain H2O2.
Q: Does EK0525 work with whole cell lysates?
A: EK0525 is not suitable for working with whole cell lysates.
Q: Does this ELISA kit contain any product produced in humans or primates?
Keywords: component, ingredient
A: None of the components in our ELISA kits are produced in humans or primates.
Q: What is the volume of the recombinant protein control? What is its shelf life?
Keywords: expiration, storage, temperature, size
A: The volume of the control is around 100pg. If unopened, the shelf life of the control is the same as the whole kit - "Store at 4˚C for 6 months, at -20˚C for 12 months." The reconstituted control can be stored at -20˚C for 2 days.
Q: Will it be okay to run the experiment if I accidentally used the wrong buffer (e.g. sample diluent buffer) for antibody dilution instead of the antibody diluent buffer? Keywords: dilution, replace, substitute, sample buffer
A: Using the sample diluent buffer for antibody dilution instead of the antibody diluent buffer will negatively affect the test result to some extent. The values of both standard and sample might be lower than the normal values.
Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead? Keywords: protocol, alternative buffer, applications, reagent recipe
A: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: Why are my O.D. values different than your values on the datasheet? Keyword: troubleshooting, reference
A: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP? Keyword: kit components, reagents, protocol
A: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine? Keyword: applications
A: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Can I calculate the results of unknown samples using a computer program with a 4-parameter logistic function? Keyword: data analysis
A: Yes, 4-parameter logistic function can be used to calculate sample data.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay? Keyword: protocol, reagents
A: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits? Keyword: technical support, help, customer service
A: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem? Keyword: troubleshoot, protocol
A: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits? Keyword: well capacity, product size
A: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do? Keyword: wash buffer
A: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals? Keyword: antibody clonality
A: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers? Keyword: storage, sequential use
A: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits? Keyword: preservative
A: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit? Keyword: positive control, negative control
A: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?
A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?
A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?
A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?
A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?
A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?
A: Yes the plate is separable. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?
A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?
A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?
A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our technical support.
Q: Can this ELISA kit react with human, mouse, rat or other species?
A: If the kit is reactive to another commonly used species (human, mouse, and/or rat), we would have listed it as a separate product. If you want to check cross-reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to human tnf alpha elisa kit, human tnf-alpha elisa kit, human tnf elisa kit, human tnf a elisa kit, human tnf-a elisa kit