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Human TNF Alpha ELISA Kit PicoKine™

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF alpha. 96wells/kit, with removable strips. Cited in 204 publication(s).

Publications (204)
Q&As (3)
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Human TNF alpha PicoKine ELISA Kit standard curve

Human TNF Alpha ELISA Kit PicoKine™ Info At A Glance

SKU:	EK0525
Size:	96wells/kit, with removable strips.
Reactivity:	Human
Sample Type:	cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume:	100μl per well
Sensitivity:	<1pg/ml
Assay Range:	15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)

Product Info

Overview

Product Name	Human TNF Alpha ELISA Kit PicoKine™ See more information about TNF-alpha, and browse related primary antibodies, ELISA kits and proteins
SKU/Catalog Number	EK0525
Storage & Handling	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Description	Detect Human Tnf Alpha/TNF with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tnf Alpha/TNF tissue specificity:
Cite This Product	Human TNF Alpha ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0525)
ELISA Validation	ELISA Validation Information

Product Specs

Reactivity/Species	Human
Applications	ELISA Our Boster Guarantee covers the use of this product in the above tested applications. *For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
Sample Type	cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sensitivity	<1pg/ml
Assay Range	15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)
Immunogen/Standard	Expression system for standard: E.coli, Immunogen sequence: V77-L233
Antibody Clonalities	Capture antibody\|Detection antibody monoclonal antibody from mouse\|polyclonal antibody from goat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Pack Size	96wells/kit, with removable strips.

Protein Target Info

Gene/Protein Basic Information For TNF (Source: Uniprot.org, NCBI)

Uniprot Id	P01375
NCBI Gene Id	7124
Species Of This Entry	Human
Gene Name	TNF
Protein Name	Tumor necrosis factor
Superfamily	Tumor necrosis factor family
Alternative Names	TNF-alpha\|APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Molecular Weight	25644
Post Tranlational Modifications	Phosphorylation; Cleavage; Oxidation; Reduction; Ubiquitination; Glycosylation; Methylation; Acetylation; Dephosphorylation; Nitration; Biotinylation; Citrullination; Ribosylation; Deacetylation; Acylation; Phosphorothioation; Iodination; Demethylation; Myristoylation; Prenylation
Subcellular Localization	Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Gene Location	6p21.33, on Chromasome Chromosome 6, gene sequence: NC_000006.12 (31575565..31578336)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

Ontology For TNF (Source: Uniprot.org, NCBI)

Protein Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T- cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity).
Interacting Proteins	Casp8; Ikbkb; IKBKG; Ripk1; Tnfaip3; TNFRSF1A; Tnfrsf1a; TNFRSF1B; Tradd; MAP3K7; RIPK1; SPPL2B; TAB2; TRADD; TRAF2
Related Diseases	Inflammation; Arthritis; Malignant Neoplasms; Rheumatoid Arthritis; Neoplasms; Tissue Adhesions; Inflammatory Response; Systemic Infection; Infective Disorder; Autoimmune Reaction; Carcinoma; Autoimmune Diseases; Septic Shock; Leukemia; Diabetes Mellitus; Crohn Disease; Pneumonia; Tuberculosis; Hiv Infections; Nervousness
Related Pathways	Pathogenesis; Secretion; Cytokine Production; Cell Death; Inflammatory Response; Immune Response; Cell Proliferation; Cell Activation; Cell Adhesion; Localization; Cell Growth; Angiogenesis; Cytokine Secretion; Phagocytosis; Bone Resorption; Sensitization; Macrophage Activation; Induction Of Apoptosis; Transport; Hypersensitivity
Background	Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Scientific References	Aggarwal, B.B. (2003). Signalling pathways of the TNF superfamily: a double-edged sword. Nature Reviews Immunology, 3, 745-756. doi: 10.1038/nri1184 Aggarwal, B.B., Moffat, B., & Harkins, R.N. (1984). Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization. Journal of Biological Chemistry, 259(1), 686-691. Balkwill, F. (2006). TNF-alpha in promotion and progression of cancer. Cancer Metastasis Reviews, 25. doi: 10.1007/s10555-006-9005-3 Carswell, E.A., Old, L.J., Kassel, R.L., Green, S., Fiore, N., & Williamson, B. (1975). An endotoxin-induced serum factor that causes necrosis of tumors. Proceedings of the National Academy of Sciences of the United States of America, 72(9), 3666-3670. doi: 10.1073/pnas.72.9.3666 Diwan, A., Tran, T., Misra, A., & Mann, D.L. (2003). Inflammatory mediators and the failing heart: a translational approach. Current Molecular Medicine, 3(2), 161-182. doi: 10.2174/1566524033361537 Dowlati, Y., Herrmann, N., Swardfager, W., Liu, H., Sham, L., Reim, E.K., & Lanctot, K.L. (2010). A meta-analysis of cytokines in major depression. Biological Psychiatry, 67(5), 446-457. doi: 10.1016/j.biopsych.2009.09.033 Hehlgans, T., & Pfeffer, K. (2005). The intriguing biology of the tumour necrosis factor/tumour necrosis factor receptor superfamily: players, rules and the games. Immunology, 115(1), 1-20. doi: 10.1111/j.1365-2567.2005.02143.x Horiuchi, T., Mitoma, H., Harashima, S., Tsukamoto, H., & Shimoda, T. (2010). Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford), 49(7), 1215-1228. doi: 10.1093/rheumatology/keq031 Lee, S.Y., Ju, M.K., Jeon, H.M., Jeong, E.K., Lee, Y.J., Kim, C.H..,?, Kang, H.S. (2018). Regulation of Tumor Progression by Programmed Necrosis. Oxidative Medicine and Cellular Longevity, 2018. doi: 10.1155/2018/3537471 Levine, B., Kalman, J., Mayer, L., Fillit, H.M., & Packer, M. (1990). Elevated circulating levels of tumor necrosis factor in severe chronic heart failure. New England Journal of Medicine, 323(4), 236-241. doi: 10.1056/NEJM199007263230405 Nagatsu, T., & Sawada, M. (2005). Inflammatory process in Parkinson's disease: role for cytokines. Current Pharmaceutical Design, 11(8), 999-1016. doi: 10.2174/1381612053381620 Parameswaran, N., & Patial, S. (2010). Tumor necrosis factor-alpha signaling in macrophages. Critical Reviews in Eukaryotic Gene Expression, 20(2), 87-103. doi: 10.1615/critreveukargeneexpr.v20.i2.10 Shrestha, B., Zhang, B., Purtha, W.E., Klein, R.S., & Diamond, M.S. (2008). Tumor necrosis factor alpha protects against lethal West Nile virus infection by promoting trafficking of mononuclear leukocytes into the central nervous system. Journal of Virology, 82(18), 8956-8964. doi: 10.1128/JVI.01118-08 Swardfager, W., Lanctot, K., Rothenburg, L., Wong, A., Cappell, J., & Herrmann, N. (2010). A meta-analysis of cytokines in Alzheimer's disease. Biology Psychiatry, 68(10), 930-941. doi: 10.1016/j.biopsych.2010.06.012 Swaroop, J.J., Rajarajeswari, D., & Naidu, J.N. (2012). Association of TNF-alpha with insulin resistance in type 2 diabetes mellitus. Indian Journal of Medical Research, 135(1), 127-130. doi: 10.4103/0971-5916.93435 Tang, P., Hung, M.C., & Klostergaard, J. (1996). Human pro-tumor necrosis factor is a homotrimer. Biochemistry, 35, 8216-8225. doi: 10.1021/bi952182t Wajant, H., Pfizenmaier, K., & Scheurich, P. (2003). Tumor necrosis factor signaling. Cell Death & Differentiation, 10, 45-65. doi: 10.1038/sj.cdd.4401189

Kit Components And Assay QC Data Details Of Human TNF Alpha ELISA Kit PicoKine™

*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.

Kit Components

Catalog number	Description	Quantity
EK0525-CAP	96-well plate precoated with anti-Human TNF-alpha antibody	1
EK0525-ST	lyophilized recombinant Human TNF-alpha standard	10ng/tube
EK0525-DA	biotinylated anti-Human TNF-alpha antibody	130ul
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul
AR1106-1	sample diluent buffer	30ml
AR1106-2	antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Materials Required But Not Provided

Microplate reader in standard size.
Automated plate washer.
Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
Clean tubes and Eppendorf tubes.
Washing buffer (neutral PBS or TBS).
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained From Human TNF Alpha ELISA Kit PicoKine™

This data is generated from a recent batch of EK0525 Human TNF Alpha ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.091	0.158	0.197	0.320	0.548	0.897	1.576	2.147

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNF Alpha ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	82	201	14	75	186
Standard deviation	0.76	5.49	12.66	0.75	5.55	11.9
CV(%)	4.8%	6.7%	7.4%	5.4%	7.4%	6.4%

Reproducibility

We measured the performance consistency of Human TNF Alpha ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	16	15	15	16	15	0.5	3.3%
Sample 2	82	91	75	89	84	6.29	7.4%
Sample 3	201	204	215	219	209	7.46	3.5%

*number of samples for each test n=16.

Specific Protocols

Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.

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Publications citing Boster Bio's EK0525 Human TNF Alpha ELISA Kit PicoKine™

Total number of citations: 204

Tang D, Kang R, Xiao W, Zhang H, Lotze Mt, Wang H, Xiao X. Am J Respir Cell Mol Biol. 2009 Dec;41(6):651-60. Doi: 10.1165/Rcmb.2008-0119Oc. Epub 2009 Mar 5. Quercetin Prevents Lps-Induced High-Mobility Group Box 1 Release And Proinflammatory Funct...	PubMed ID 19265175
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.	PubMed ID 23382627
Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli...	PubMed ID 24685824
Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo...	PubMed ID 23642811
Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis.	PubMed ID 25044235
Wang G, Ji Y, Li Z, Han X, Guo N, Song Q, Quan L, Wang T, Han W, Pang D, Ouyang H, Tang X. Sci Rep. 2014 May 9;4:4905. Doi: 10.1038/Srep04905. Nitro-Oleic Acid Downregulates Lipoprotein-Associated Phospholipase A2 Expression Via The P42/P44 Mapk A...	PubMed ID 24809325
Han X, Wang T, Zhang J, Liu X, Li Z, Wang G, Song Q, Pang D, Ouyang H, Tang X. Biol Open. 2015 Apr 2;4(5):661-5. Doi: 10.1242/Bio.201410900. Apolipoprotein Ciii Regulates Lipoprotein-Associated Phospholipase A2 Expression Via The Mapk And Nf??b Pa...	PubMed ID 25836672
Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????	PubMed ID 25428340
Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...	PubMed ID 24593676
Tong Gq, Zhang Zh, Zhao Y, Liu Jj, Han Jb. Arch Environ Contam Toxicol. 2015 Jan;68(1):31-7. Doi: 10.1007/S00244-014-0077-8. Epub 2014 Sep 7. Traffic-Related Pm2.5 Induces Cytosolic [Ca?????] Increase Regulated By Orai1, Alters The Can-Nfat Signal...	PubMed ID 25194241
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.	PubMed ID 24571896
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...	PubMed ID 25744647
Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...	PubMed ID 24368037
Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And...	PubMed ID 25953665
Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.	PubMed ID 24738018
Feng X, Lv C, Wang F, Gan K, Zhang M, Tan W. Clin Dev Immunol. 2013;2013:160123. Doi: 10.1155/2013/160123. Epub 2013 Nov 18. Modulatory Effect Of 1,25-Dihydroxyvitamin D 3 On Il1 ?? -Induced Rankl, Opg, Tnf ?? , And Il-6 Expression In Human Rheuma...	PubMed ID 24348674
Li P, Wang B, Sun F, Li Y, Li Q, Lang H, Zhao Z, Gao P, Zhao Y, Shang Q, Liu D, Zhu Z. Sci Rep. 2015 May 28;5:10229. Doi: 10.1038/Srep10229. Mitochondrial Respiratory Dysfunctions Of Blood Mononuclear Cells Link With Cardiac Disturbance In Patient...	PubMed ID 26018291
Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.	PubMed ID 25880347
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.	PubMed ID 22095388
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.	PubMed ID 25626689
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...	PubMed ID 26022197
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.	PubMed ID 25383758
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...	PubMed ID 25725585
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.	PubMed ID 24710706
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...	PubMed ID 23746956
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.	PubMed ID 24955162
Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.	PubMed ID 23786840
Zheng Ls, Kaneko N, Sawamoto K. Front Cell Neurosci. 2015 Jan 28;9:5. Doi: 10.3389/Fncel.2015.00005. Ecollection 2015. Minocycline Treatment Ameliorates Interferon-Alpha- Induced Neurogenic Defects And Depression-Like Behaviors In Mice.	PubMed ID 25674053
Fan Z, Jing H, Yao J, Li Y, Hu X, Shao H, Shen G, Pan J, Luo F, Tian X. Oxid Med Cell Longev. 2014;2014:191624. Doi: 10.1155/2014/191624. Epub 2014 Aug 20. The Protective Effects Of Curcumin On Experimental Acute Liver Lesion Induced By Intestinal...	PubMed ID 25215173
Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, Shi G. Plos One. 2014 Apr 21;9(4):E95931. Doi: 10.1371/Journal.Pone.0095931. Ecollection 2014. Anti-Inflammatory Activity Of N-Butanol Extract From Ipomoea Stolonifera In Vivo And In Vitro.	PubMed ID 24752203
Xie Wl, Shi Q, Zhang J, Zhang By, Gong Hs, Guo Y, Wang Sb, Xu Y, Wang K, Chen C, Liu Y, Dong Xp. J Mol Neurosci. 2013 Nov;51(3):919-32. Doi: 10.1007/S12031-013-0002-Z. Epub 2013 Mar 24. Abnormal Activation Of Microglia Accompanied With Disrupted C...	PubMed ID 23526370
Lu Dh, Guo Xy, Qin Sy, Luo W, Huang Xl, Chen M, Wang Jx, Ma Sj, Yang Xw, Jiang Hx. World J Gastroenterol. 2015 Feb 7;21(5):1531-45. Doi: 10.3748/Wjg.V21.I5.1531. Interleukin-22 Ameliorates Liver Fibrogenesis By Attenuating Hepatic Stellate Cell Ac...	PubMed ID 25663772
Jin Y, Zhang Y, Wan C, Wang H, Hou L, Chang J, Fan K, Xie X. Evid Based Complement Alternat Med. 2013;2013:194976. Doi: 10.1155/2013/194976. Epub 2013 Sep 18. Immunomodulatory Activity And Protective Effects Of Polysaccharide From Eupatorium Adeno...	PubMed ID 24159339
Ni J, Wang X, Chen S, Liu H, Wang Y, Xu X, Cheng J, Jia J, Zhen X. Brain Behav Immun. 2015 Oct;49:75-85. Doi: 10.1016/J.Bbi.2015.04.014. Epub 2015 Apr 29. Microrna Let-7C-5P Protects Against Cerebral Ischemia Injury Via Mechanisms Involving The In...	PubMed ID 25934573
Zhang F, Li Zl, Xu Xm, Hu Y, Yao Jh, Xu W, Jing Hr, Wang S, Ning Sl, Tian Xf. Mol Med Rep. 2015 Jan;11(1):269-76. Doi: 10.3892/Mmr.2014.2679. Epub 2014 Oct 16. Protective Effects Of Icariin-Mediated Sirt1/Foxo3 Signaling Pathway On Intestinal Isch...	PubMed ID 25323330
Sun Ln, Yang Zy, Lv Ss, Liu Xc, Guan Gj, Liu G. Int Immunopharmacol. 2014 Nov;23(1):236-46. Doi: 10.1016/J.Intimp.2014.08.023. Epub 2014 Sep 4. Curcumin Prevents Diabetic Nephropathy Against Inflammatory Response Via Reversing Caveolin-1 Tyr14 Pho...	PubMed ID 25196431
Xu W, Lu Y, Yao J, Li Z, Chen Z, Wang G, Jing H, Zhang X, Li M, Peng J, Tian X. Shock. 2014 Nov;42(5):440-7. Doi: 10.1097/Shk.0000000000000225. Novel Role Of Resveratrol: Suppression Of High-Mobility Group Protein Box 1 Nucleocytoplasmic Transloca...	PubMed ID 25004063
Wei Z, Yang Y, Xie C, Li C, Wang G, Ma L, Xiang M, Sun J, Wei Y, Chen L. Fitoterapia. 2014 Sep;97:172-83. Doi: 10.1016/J.Fitote.2014.06.002. Epub 2014 Jun 9. Synthesis And Biological Evaluation Of Pyranoisoflavone Derivatives As Anti-Inflammatory ...	PubMed ID 24924289
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Wen Y, Gan H, Li Z, Sun X, Xiong Y, Xia Y Sci Rep. 2018 Apr 13;8(1):5941. doi: 10.1038/s41598-018-24397-w. Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis	PubMed ID 29654308
Qin S, Gao Z, Liu Y, Liu C, Wang J, Zou LL. Oncol Lett. 2018 Feb;15(2):1630-1638. doi: 10.3892/ol.2017.7477. Epub 2017 Nov 23. Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cel...	PubMed ID 29434859
Liu B, Piao X, Guo L, Wang G, Sun W, Gao L, Zheng X, Fang Y. Evid Based Complement Alternat Med. 2017;2017:7323129. doi: 10.1155/2017/7323129. Epub 2017 Nov 20. A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylat...	PubMed ID 29358969
Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, Lin W, Zhang F. Oxid Med Cell Longev. 2017;2017:6905217. doi: 10.1155/2017/6905217. Epub 2017 Nov 19. Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation o...	PubMed ID 29359008
Huang N, Xu Y, Zhou H, Lin D, Zhang B, Zhang Y, Pan D, Tao L, Liu X, Shen X. Med Sci Monit. 2017 Oct 4;23:4760-4767. Essential Oil from Fructus Alpiniae Zerumbet Protects Human Umbilical Vein Endothelial Cells In Vitro from Injury Induced by High ...	PubMed ID 28976943
Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals	PubMed ID 29114515
Xu B, Zhang P, Li W, Liu R, Tang J, Fan H. Front Microbiol. 2017 Aug 9;8:1524. doi: 10.3389/fmicb.2017.01524. eCollection 2017. hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Surviva...	PubMed ID 28848531
Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. Prion protein is required for tumor necrosis factor α (TNFα)-triggered nuclear factor κB (NF-κB) signaling an...	PubMed ID 28900035
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome	PubMed ID 2865457
The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophages.	PubMed ID 17617616
Identification and Characterization of the Lamprey High-Mobility Group Box 1 Gene	PubMed ID 22563397
Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy	PubMed ID 22787172
Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients	PubMed ID 24250536
Evaluation of salivary tumor necrosis factor-alpha in patients with the chronic periodontitis: A case-control study	PubMed ID 24554882
Role of Pentoxifylline and Sparfloxacin in Prophylaxis of Spontaneous Bacterial Peritonitis in Cirrhotic Patients	PubMed ID 24729879
Protective Effect of Resveratrol against IL-1?-Induced Inflammatory Response on Human Osteoarthritic Chondrocytes Partly via the TLR4/MyD88/NF-?B Signaling Pathway: An ?in VitroStudy?	PubMed ID 24758933
RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms	PubMed ID 25254379
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages	PubMed ID 25861639
Polyunsaturated fatty acids augment tumoricidal action of 5-fluorouracil on gastric cancer cells by their action on vascular endothelial growth factor, tumor necrosis factor-? and lipid metabolism related factors	PubMed ID 25995742
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155	PubMed ID 26191144
Evaluation of adenosine deaminase (ADA) isoenzymes activity and tumor necrosis factor-? (TNF?) concentration in chronic heart failure	PubMed ID 26417242
Metabolic changes of H2S in smokers and patients of COPD which might involve in inflammation, oxidative stress and steroid sensitivity	PubMed ID 26455818
Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion	PubMed ID 26530694
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction	PubMed ID 26619044
A deleterious role for Th9/IL-9 in hepatic fibrogenesis	PubMed ID 26728971
Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis	PubMed ID 26962319
Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP)	PubMed ID 27127347
Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-? and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy	PubMed ID 27218216
4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner	PubMed ID 27276712
The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B cepacia complex-infected Cystic Fibrosis patients	PubMed ID 27325348
Comparison of Serum Levels of Vitamin D and Inflammatory Markers Between Women With Gestational Diabetes Mellitus and Healthy Pregnant Control	PubMed ID 27385967
NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation.	PubMed ID 27403035
Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease	PubMed ID 27445436
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling	PubMed ID 27590510
Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis	PubMed ID 27602373
Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial	PubMed ID 27651815
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?	PubMed ID 27673564
Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage	PubMed ID 27698749
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells	PubMed ID 27765913
Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-?B and p38 MAPK	PubMed ID 28105094
Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine	PubMed ID 28388901
Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery	PubMed ID 28450928
Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells	PubMed ID 28498464
Effect of Helix aspersa extract on TNF?, NF-?B and some tumor suppressor genes in breast cancer cell line Hs578T	PubMed ID 28539722
Wogonoside inhibits invasion and migration through suppressing TRAF2/4 expression in breast cancer	PubMed ID 28774312
Tauroursodeoxycholic acid attenuates endoplasmic reticulum stress and protects the liver from chronic intermittent hypoxia induced injury	PubMed ID 28962181
Dynamic changes of ?-melanocyte-stimulating hormone levels in the serum of patients with craniocerebral trauma	PubMed ID 28962188
Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-?B activation in LPS-stimulated murine macrophages	PubMed ID 29179440
Inhibiting PSM?-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system	PubMed ID 29500427
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis	PubMed ID 29805485
Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis	PubMed ID 26673286
Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways	PubMed ID 30015892
Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation	PubMed ID 28511963
The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility	PubMed ID 29914245
Catalpol ameliorates diabetic atherosclerosis in diabetic rabbits	PubMed ID 27830011
Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes	PubMed ID 26239742
Small molecule GL-V9 protects against colitis-associated colorectal cancer by limiting NLRP3 inflammasome through autophagy	PubMed ID 29296531
Establishment of a Rabbit Model of Chronic Obstructive Sleep Apnea and Application in Cardiovascular Consequences	PubMed ID 28218220
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome	PubMed ID 20346168
Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats	PubMed ID 27576501
Inhibition of inflammation using diacerein markedly improved renal function in endotoxemic acute kidney injured mice	PubMed ID 30140293
E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury	PubMed ID 28740545
Protection of SAL B with H9C2 cells	PubMed ID 26705025
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis	PubMed ID 27152709
Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia	PubMed ID 25627661
Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury	PubMed ID 29188738
Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026	PubMed ID 25412187
Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026	PubMed ID 25412187
Cytotoxic, antitumor and immunomodulatory effects of the water-soluble polysaccharides from lotus (Nelumbo nucifera Gaertn.) seeds	PubMed ID 27827862
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Immunologic and psychologic parameters associated with geographic tongue	PubMed ID 24842481
Cytokine, chemokine and BDNF levels in medication-free pediatric patients with obsessive-compulsive disorder.	PubMed ID 29302747
Investigation of the maternal and cord plasma levels of IL-1 beta, TNF-alpha and VEGF in early membrane rupture	PubMed ID 26364900
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Customer Q&As

Questions and answers from customers related to EK0525 Human TNF Alpha ELISA Kit PicoKine™

3 Related Questions

Question

Does EK0525 work with whole cell lysates?

Verified Customer

Asked: 2017-06-05

Answer

EK0525 is not suitable for working with whole cell lysates.

Boster Scientific Support

Answered: 2017-06-05

Question

For EK0525, a customer wanted to know if the kit would work on Cell lysate samples. They also wanted to know if the kit would work with Hep2G cell supernatant.

Verified Customer

Asked: 2014-09-04

Answer

The immunogen sequence of the antibodies in the kits is V77-L233 which is the soluble form of TNF alpha. The protein is synthesized in the intracellular space of cells and secretes into the extracellular space which is cell culture supernatant. And the kit might be able to detect patial intracellular secreted protein which hasn't released into extracellular space in cell lysate. The kit would work with Hep2G cell supernatant.

Boster Scientific Support

Answered: 2014-09-04

Question

I want to know more about your product EK0525 Human TNF Alpha ELISA Kit on the sensitivity of the assay. Do you have a detectable analytic range for urine samples? If not for urine then for serum?

Verified Customer

Asked: 2013-03-29

Answer

Our product EK0525 Human TNF Alpha ELISA Kit is not specifically validated with urine (only serum, plasma, and cell culture supernatants). However, we do not have concentration levels to report. When we tested human serum with EK0525 Human TNF Alpha ELISA Kit we found the measured levels were below the sensitivity limit which is 20 pg/ml not 1pg/ml as specified in the protocol. Thus, if testing body fluids, we would recommend using EK0525 Human TNF Alpha ELISA Kit mainly for spiking and recovery studies which have expected elevated TNF-alpha levels than normal.

Boster Scientific Support

Answered: 2013-03-29

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Human TNF Alpha ELISA Kit PicoKine™

Human TNF Alpha ELISA Kit PicoKine™ Info At A Glance

Overview

Product Specs

Gene/Protein Basic Information For TNF (Source: Uniprot.org, NCBI)

Ontology For TNF (Source: Uniprot.org, NCBI)

Kit Components And Assay QC Data Details Of Human TNF Alpha ELISA Kit PicoKine™

Reproducibility

Specific Protocols

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Total number of citations: 204

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