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Product Info Summary
| SKU: | EK0525 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
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Product info
Product Name
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™
SKU/Catalog Number
EK0525
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0525)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V77-L233
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (serum, plasma)
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0525 is reactive to TNF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0525 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0525-CAP | Anti-Human TNF Pre-coated 96-well strip microplate | 1 |
| EK0525-ST | Human TNF Standard | 2 vials, 10 ng/tube |
| EK0525-DA | Human TNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.091 | 0.158 | 0.197 | 0.320 | 0.548 | 0.897 | 1.576 | 2.047 |
Data Example Images
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Human TNF alpha PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNF-alpha, Dilution ratio of 1:1, concentration in serum is around 100pg/ml.
Some articles we found to cite concentrations of TNF-alpha in samples: 24812899 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 82 | 201 | 14 | 75 | 186 |
| Standard deviation | 0.76 | 5.49 | 12.66 | 0.75 | 5.55 | 11.9 |
| CV (%) | 4.8% | 6.7% | 7.4% | 5.4% | 7.4% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TNF-alpha in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 15 | 15 | 16 | 15 | 0.5 | 3.3% |
| Sample 2 | 82 | 91 | 75 | 89 | 84 | 6.29 | 7.4% |
| Sample 3 | 201 | 204 | 215 | 219 | 209 | 7.46 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For TNF (Source: Uniprot.Org, NCBI)
Uniprot ID
P01375
Gene ID
7124
Gene Name
TNF
Full Name
Tumor necrosis factor
Weight
25.644kDa
Superfamily
Tumor necrosis factor family
Alternative Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNF, check out the TNF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (EK0525)
EK0525 has been cited in 211 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu F,Chen H,Hong Y,Lin Y,Liu L,Wei N,Wu Q,Liao S,Yang S,He J,Shao Y.A gain-of-function NLRP3 3‘-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression.Sci Rep.2021 Jun 25;11(1):13300.doi:10.1038/s41598-021-92547-8.PMID: 34172780.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PLASMA, HUVECS AND THP-1 CELL, DILUTION:NA
Singh A,Das D,Kurra S,Arava S,Gupta S,Sharma A.Dendritic cells and their associated pro-inflammatory cytokines augment to the inflammatory milieu in vitiligo skin.Cytokine.2021 Jun 5:155598.doi:10.1016/j.cyto.2021.155598.Epub ahead of print.PMID:34103210.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:DENDRITIC CELL, DILUTION:NA
Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0525 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION:NA
Abo-Youssef SM,Allam AH,Mustafa MS,Abd el Hamid HS,Shahat AK,Moussa HH.Expression level of micro-RNA-1, IL-5,IFN γ, and TNFα in adult-onset asthmatic patients.Egypt J Chest Dis Tuberc 2021;70:215-22
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
Species: Human,Mouse
EK0525 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA
Erica Costantini,Bruna Sinjari,Katia Falasca,Marcella Reale,Sergio Caputi,Srinivas Jagarlapodii,Giovanna Murmura," Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast",Mediators of Inflammation,vol.2021,Article ID 5562340,9 pages,2021. https://doi.org/10.1155/2021/5562340
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HGF CELL, DILUTION:NA
Pınar Bürhan-Çavuşoğlu,Evin İşcan,Ayşim Güneş,Neşe Atabey,Tunç Alkın,Increased telomerase activity in major depressive disorder with melancholic features: Possible role of pro-inflammatory cytokines and the brain-derived neurotrophic factor,Brain,Behavior,& Immunity - Health,2021,100259,ISSN 2666-3546,https://doi.org/10.1016/j.bbih.2021.100259.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Yang N,Wang H,Zhang R,Niu Z,Zheng S,Zhang Z.C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway.Arch Med Res.2021 May 1:S0188-4409(21)00079-5.doi:10.1016/j.arcmed.2021.03.008.Epub ahead of print.PMID:33947580.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA
Ma Z,Li K,Chen P,Pan J,Li X,Zhao G.Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury.Biol Pharm Bull.2020;43(10):1481-1489.doi:10.1248/bpb.b20-00050.PMID:32999158.
Species: Mouse
EK0525 usage in article: APP:ELISA, SAMPLE:CORTICAL NEURONS, DILUTION:NA
Lou A,Wang L,Lai W,Zhu D,Wu W,Wang Z,Cai Z,Yang M.Advanced oxidation protein products induce inflammatory responses and invasive behaviour in fibroblast-like synoviocytes via the RAGE-NF-κB pathway.Bone Joint Res.2021 Apr;10(4):259-268.doi:10.1302/2046-3758.104.BJR-2020-0085.R2.PMID:33827262.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:RA-FLS, DILUTION:NA
Yang M, Wang LI. MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis. J Biosci. 2021;46:28. PMID: 33753579.
Species: Human,Rat
EK0525 usage in article: APP:ELISA, SAMPLE:B/TSMCS, DILUTION:NA
Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021 Mar 12. doi: 10.1007/s10753-021-01445-8. Epub ahead of print. PMID: 33710445.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA
Li C, Liu M, Liu K, Li M, Liu Y, Li T, Wei Y, Long Y, He W, Shi X, Li Y, Zhang H. BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody. Biochem Biophys Res Commun. 2021 Mar 16;551:155-160. doi: 10.1016/j.bbrc.2021.02.128. Epub ahead of print. PMID: 33740622.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0525 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA
Guo L,Jiang B,Li D,Xiao X. Nephroprotective Effect of Adropinin Against Streptozotocin-Induced Diabetic Nephropathy in Rats: Inflammatory Mechanism and YAP/TAZ Factor.Drug Des Devel Ther.2021 Feb 16;15:589-600.doi:10. 2147/DDDT.S294009.PMID:33623368;PMCID
Species: Rat
EK0525 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Chen M,Chen Z,Huang D,Sun C,Xie J,Chen T,Zhao X,Huang Y,Li D,Wu B,Wu D.Myricetin inhibits TNF-α-induced inflammation in A549 cells via the SIRT1/NF-κB pathway.Pulm Pharmacol Ther.2021 Feb 15:102000.doi:10.1016/j.pupt.2021.102000.Epub ahead of print.PMID:3
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:A549 CELL, DILUTION:NA
Huang C,Chen Y,Lai B,Chen YX,Xu CY,Liu YF.Overexpression of SP1 restores autophagy to alleviate acute renal injury induced by ischemia-reperfusion through the miR-205/PTEN/Akt pathway.J Inflamm (Lond).2021 Feb 5;18(1):7.doi:10.1186/s12950-021-00270-y.PMID
Species: Human,Rat
Junde Hou,Yanxin Cheng,Hongfang Wei et al.Dexmedetomidine Attenuates Hemodynamic and Proinflammatory Responses During Craniotomy for Traumatic Brain Injury:A Randomized Controlled Trial Study,02 February 2021,PREPRINT (Version 1) available at Research Squ
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Qinchao Chen,Meng Cao,Hanyi Ge,"Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis",BioMed Research International,vol.2021,Article ID 8899863,12 pages,2021.https://doi.org/10.1155/2021/8899863
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA
Sharma V,Sonkar SC,Singhal P,Kumar A,Singh RK,Ramachandran VG,Hariprasad R,Saluja D,Bharadwaj M.Functional impact of allelic variations/haplotypes of TNF-α on reproductive tract infections in Indian women.Sci Rep.2021 Jan 12;11(1):627.doi:10.1038/s41598-0
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Gao H,Guo Z. LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Med (Wars). 2021 Jan 8;16(1):117-127.doi:10.1515/med-2021-0200.PMID:33542956;PMCID: PMC7819546.
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA
Wei W,Yao Y,Bi H,Xu W,Gao Y.Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.Biochem Biophys Res Commun.2021 Jan 8;540:1-7.doi:10.1016/j.bbrc.2020.12.018.Epub ahead of print.PMID:33429194.
Species: Human
Javid Sadri Nahand,Khadijeh Khanaliha,Hamed Mirzaei et al.Anoikis Resistance and Development in Prostate Cancer: The Possible Role of HPV/EBV Coinfection, 04 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Mao H,Huang Q,Liu Y. MEG3 aggravates hypoxia/reoxygenation induced apoptosis of renal tubular epithelial cells via the miR-129-5p/HMGB1 axis. J Biochem Mol Toxicol.2020 Nov 11:e22649.doi:10.1002 /jbt .22 649. Epub ahead of print.PMID:33175458.
Species: Human,Rat
EK0525 usage in article: APP:ELISA, SAMPLE:HK-2 CELLS, DILUTION:NA
Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA
Xue M, Peng N, Zhu X, Zhang H. Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis. Biochem Biophys Res Commun. 2020 Nov 25:S0006-291X(20)32086-6. doi
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HPMECS AND BEAS-2B CELLS, DILUTION:NA
Das D, Singh A, Antil PS, Sharma D, Arava S, Khandpur S, Sharma A. Distorted frequency of dendritic cells and their associated stimulatory and inhibitory markers augment the pathogenesis of pemphigus vulgaris. Immunol Res. 2020 Nov 12. doi: 10.1007/s12026
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:PBMCS, DILUTION:NA
Asila A, Wang Y, Miao D, Yang X, Kaisaer Y, Ma L, Lv G, Yang C. SNHG16/miR-485-5p/BMP7 axis modulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Gene Med. 2020 Nov 11:e3296. doi: 10.1002/jgm.3296. Epub ahead of print
Species: Human
EK0525 usage in article: APP:ELISA, SAMPLE:HBMSCS, DILUTION:NA
Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.
Tian, L. X., Tang, X., Zhu, J. Y., Luo, L., Ma, X. Y., Cheng, S. W., ... & Lv, C. Z. (2020). Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis. Cell Communication and Signaling, 18, 1-16.
Duan, Y., Zheng, H., Li, Z., Yao, Y., Ding, J., Wang, X., ... & Zheng, X. (2020). Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis. Biomaterials, 120012.
Investigation of the maternal and cord plasma levels of IL-1 beta, TNF-alpha and VEGF in early membrane rupture
Sublytic C5b-9 Induces Glomerular Mesangial Cell Apoptosis through the Cascade Pathway of MEKK2%u2013p38 MAPK%u2013IRF-1%u2013TRADD%u2013Caspase 8 in Rat Thy-1 %u2026
Cytotoxic, antitumor and immunomodulatory effects of the water-soluble polysaccharides from lotus (Nelumbo nucifera Gaertn.) seeds
Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation
Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways
Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis
Inhibiting PSM?-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system
Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-?B activation in LPS-stimulated murine macrophages
Dynamic changes of ?-melanocyte-stimulating hormone levels in the serum of patients with craniocerebral trauma
Tauroursodeoxycholic acid attenuates endoplasmic reticulum stress and protects the liver from chronic intermittent hypoxia induced injury
Wogonoside inhibits invasion and migration through suppressing TRAF2/4 expression in breast cancer
Effect of Helix aspersa extract on TNF?, NF-?B and some tumor suppressor genes in breast cancer cell line Hs578T
Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells
Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery
Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine
Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-?B and p38 MAPK
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells
Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage
miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?
Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial
Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease
NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation.
Comparison of Serum Levels of Vitamin D and Inflammatory Markers Between Women With Gestational Diabetes Mellitus and Healthy Pregnant Control
The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B cepacia complex-infected Cystic Fibrosis patients
4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner
Elevated expression levels of serum insulin-like growth factor-1, tumor necrosis factor-? and vascular endothelial growth factor 165 might exacerbate type 2 diabetic nephropathy
Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP)
Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis
A deleterious role for Th9/IL-9 in hepatic fibrogenesis
Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction
Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion
Metabolic changes of H2S in smokers and patients of COPD which might involve in inflammation, oxidative stress and steroid sensitivity
Evaluation of adenosine deaminase (ADA) isoenzymes activity and tumor necrosis factor-? (TNF?) concentration in chronic heart failure
Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155
Polyunsaturated fatty acids augment tumoricidal action of 5-fluorouracil on gastric cancer cells by their action on vascular endothelial growth factor, tumor necrosis factor-? and lipid metabolism related factors
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages
RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms
Protective Effect of Resveratrol against IL-1?-Induced Inflammatory Response on Human Osteoarthritic Chondrocytes Partly via the TLR4/MyD88/NF-?B Signaling Pathway: An ?in VitroStudy?
Role of Pentoxifylline and Sparfloxacin in Prophylaxis of Spontaneous Bacterial Peritonitis in Cirrhotic Patients
Evaluation of salivary tumor necrosis factor-alpha in patients with the chronic periodontitis: A case-control study
Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients
Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy
Identification and Characterization of the Lamprey High-Mobility Group Box 1 Gene
The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophages.
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome
Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. Prion protein is required for tumor necrosis factor α (TNFα)-triggered nuclear factor κB (NF-κB) signaling an...
Xu B, Zhang P, Li W, Liu R, Tang J, Fan H. Front Microbiol. 2017 Aug 9;8:1524. doi: 10.3389/fmicb.2017.01524. eCollection 2017. hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Surviva...
Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals
Huang N, Xu Y, Zhou H, Lin D, Zhang B, Zhang Y, Pan D, Tao L, Liu X, Shen X. Med Sci Monit. 2017 Oct 4;23:4760-4767. Essential Oil from Fructus Alpiniae Zerumbet Protects Human Umbilical Vein Endothelial Cells In Vitro from Injury Induced by High ...
Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, Lin W, Zhang F. Oxid Med Cell Longev. 2017;2017:6905217. doi: 10.1155/2017/6905217. Epub 2017 Nov 19. Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation o...
Liu B, Piao X, Guo L, Wang G, Sun W, Gao L, Zheng X, Fang Y. Evid Based Complement Alternat Med. 2017;2017:7323129. doi: 10.1155/2017/7323129. Epub 2017 Nov 20. A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylat...
Qin S, Gao Z, Liu Y, Liu C, Wang J, Zou LL. Oncol Lett. 2018 Feb;15(2):1630-1638. doi: 10.3892/ol.2017.7477. Epub 2017 Nov 23. Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cel...
Wen Y, Gan H, Li Z, Sun X, Xiong Y, Xia Y Sci Rep. 2018 Apr 13;8(1):5941. doi: 10.1038/s41598-018-24397-w. Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis
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Customer Q&As
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18 Customer Q&As for Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™
Question
The EK0525 manual indicates sensitivity as <1 pg/ml. Is it possible to see the signal difference at small concentrations using more sensitive detection reagent?
Verified customer
Asked: 2021-01-18
Answer
The sensitivity is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration. At the sensitivity level, the kit can detect the presence of the protein but not accurately quantify it. Accurate quantitation begins at the lowest point on the standard curve. For the Human TNF Alpha ELISA Kit PicoKine™ (EK0525), the values lower than 15.6pg/ml might not be reliable. If the user would like to see the signal difference at small concentrations, ELISA kits with higher sensitivity provided by other vendors are suggested to be used.
Boster Scientific Support
Answered: 2021-01-19
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TNF-alpha in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-12-09
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TNF-alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-12-09
Question
Can EK0525 be used in calculating the tnf alpha concentration in human gingival cervicular fluid?
Verified customer
Asked: 2020-10-13
Answer
The Human TNF Alpha ELISA Kit PicoKine™ (EK0525) hasn't been tested with human gingival cervicular fluid.We suggest to run a pilot test for this. In theory, if the expression level of the target protein in the urine samples falls in the assay range of the kits, the kits are able to detect the protein in the sample.
Boster Scientific Support
Answered: 2020-10-14
Question
What dilution is needed for human serum samples?
Verified customer
Asked: 2020-09-25
Answer
For human serum samples using the Human TNF Alpha ELISA Kit PicoKine™ (EK0525), dilution of 1:1 is recommended.
Boster Scientific Support
Answered: 2020-09-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-06
Question
What is the expiration date of EK0525 from the time of manufacturing, 6 months or 1 year?
Verified customer
Asked: 2019-12-17
Answer
Expiration is based on storage temperature. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Boster Scientific Support
Answered: 2019-12-17
Question
Will EK0525 work on Hep2G cell supernatant?
Verified customer
Asked: 2019-10-15
Answer
Yes, the Human TNF Alpha ELISA Kit PicoKine™ (EK0525) would work with Hep2G cell supernatant.
Boster Scientific Support
Answered: 2019-10-16
Question
Will EK0525 work on Cell lysate samples?
Verified customer
Asked: 2019-10-15
Answer
The immunogen sequence of the antibodies in the Human TNF Alpha ELISA Kit PicoKine™ (EK0525) is V77-L233 which is the soluble form of TNF alpha. The protein is synthesized in the intracellular space of cells and secretes into the extracellular space which is cell culture supernatant. And the kit might be able to detect partial intracellular secreted protein which has not released into extracellular space in cell lysate.
Boster Scientific Support
Answered: 2019-10-16
Question
Q: Can TNF-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-19
Question
What is the assay range of EK0525 in cell culture supernates sample?
Verified customer
Asked: 2019-05-09
Answer
The assay range of the Human TNF Alpha ELISA Kit PicoKine™ (EK0525) human TNF alpha kit in cell culture supernates is 15.6pg/ml-1000pg/ml.
Boster Scientific Support
Answered: 2019-05-10
Question
What is the assay range of EK0525 in serum and plasma samples?
Verified customer
Asked: 2019-05-09
Answer
The assay range of the Human TNF Alpha ELISA Kit PicoKine™ (EK0525) human TNF alpha kit in serum and plasma samples is 7.8pg/ml-500pg/ml.
Boster Scientific Support
Answered: 2019-05-10
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
N. Phillips
Verified customer
Asked: 2019-04-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-04-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TNF-alpha using the EK0525 Human TNF-alpha Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-03-14
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-07-31
Question
Does EK0525 work with whole cell lysates?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
EK0525 is not suitable for working with whole cell lysates.
Boster Scientific Support
Answered: 2017-06-05
Question
Q: how to analyze ELISA data? I have obtained TNF-alpha level in plasma.
Verified Customer
Verified customer
Asked: 2017-01-13
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-01-13
Question
For EK0525, a customer wanted to know if the kit would work on Cell lysate samples. They also wanted to know if the kit would work with Hep2G cell supernatant.
Verified Customer
Verified customer
Asked: 2014-09-04
Answer
The immunogen sequence of the antibodies in the kits is V77-L233 which is the soluble form of TNF alpha. The protein is synthesized in the intracellular space of cells and secretes into the extracellular space which is cell culture supernatant. And the kit might be able to detect patial intracellular secreted protein which hasn't released into extracellular space in cell lysate. The kit would work with Hep2G cell supernatant.
Boster Scientific Support
Answered: 2014-09-04
Question
I want to know more about your product EK0525 Human TNF Alpha ELISA Kit on the sensitivity of the assay. Do you have a detectable analytic range for urine samples? If not for urine then for serum?
Verified Customer
Verified customer
Asked: 2013-03-29
Answer
Our product EK0525 Human TNF Alpha ELISA Kit is not specifically validated with urine (only serum, plasma, and cell culture supernatants). However, we do not have concentration levels to report. When we tested human serum with EK0525 Human TNF Alpha ELISA Kit we found the measured levels were below the sensitivity limit which is 20 pg/ml not 1pg/ml as specified in the protocol. Thus, if testing body fluids, we would recommend using EK0525 Human TNF Alpha ELISA Kit mainly for spiking and recovery studies which have expected elevated TNF-alpha levels than normal.
Boster Scientific Support
Answered: 2013-03-29
