Boster Bio Reporter-Labeled Cell Lines

Luciferase Reporter Cell Lines for Pathway Activity Assays

Ready-to-use Renilla luciferase reporter cell lines for measuring pathway activation, promoter response, receptor signaling, and compound effects in functional cell-based assays.

Ready-to-use validated reporter cell lines
Quantitative luciferase pathway readout
Custom reporter cell line generation available

Browse catalog reporter cell lines by pathway and cell background, or request a custom model for your assay.

Browse Ready-to-Use Reporter Cell Lines Request Custom Cell Line

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Select Reporter Cell Lines by Pathway, Readout, and Cell Background

Boster Bio reporter cell lines support pathway activity assays, compound-response studies, transcription factor activity analysis, and custom functional cell-based assay development.

Pathway Activity

Monitor signaling responses through reporter systems such as NF-kB, ISRE, STAT, TLR/NF-kB, SBE, CRE, NFAT, and AP-1.

Functional Readout

Use Renilla luciferase luminescence to quantify promoter activation, receptor signaling, and compound effects.

Cell Backgrounds

Choose from common research cell backgrounds including HEK293, Jurkat, RAW264.7, HeLa, Ba/F3, MCF7, and NIH 3T3.

Custom Models

Request custom reporter cell line generation when your assay requires a specific promoter, response element, receptor, or host cell.

Measure Pathway Activity Without Building a Reporter Model from Scratch

Ready-to-use stable reporter cell lines give your team a faster starting point for functional assays, pathway activity studies, and compound-response screening.

Reporter cell lines help researchers convert pathway activation, promoter response, or receptor-mediated signaling into a quantitative luminescent readout. Instead of transfecting and validating a reporter construct for every experiment, ready-to-use stable reporter cell lines help reduce setup time and improve assay consistency.

Use this page to select a catalog reporter cell line by pathway and cell background, or request a custom reporter cell line when your assay requires a specific promoter, response element, receptor, or host cell model.

Choose Reporter Cell Lines by Assay Goal

Start with the biological question first, then narrow by pathway, response element, and cell background.

Inflammation & Innate Immunity

Consider NF-kB, TLR/NF-kB, ISRE, iNOS, TNF-α, or IL-8 reporter cell lines.

Cytokine Signaling

Consider STAT1, STAT3, STAT4, STAT5, NFAT, AP-1, or cytokine-responsive reporter models.

Compound Screening

Use stable reporter cell lines to compare activators, inhibitors, biologics, and small molecules.

Custom Assay Models

Request a custom reporter cell line for a target pathway, promoter, receptor, or cell background not listed.

Browse Ready-to-Use Renilla Luciferase Reporter Cell Lines

Select from validated reporter cell lines across common pathway readouts and cell backgrounds. Each product page includes catalog details, application information, and available validation figures.

Product

Figure

Pathway / Response

Application

Price / Format

STAT4 Luciferase Reporter-Ba/F3 Cell Line

Ba/F3 reporter model for STAT4 pathway activity studies.

1 image

STAT4

Functional Assay

$5040
Cat # RC1045
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

FOXP3 Luciferase Reporter-Jurkat Cell Line

Jurkat reporter model for FOXP3 transcriptional response studies.

1 image

FOXP3

Functional Assay

$2072
Cat # RC1044
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

ISRE Luciferase Reporter-HEK293 Cell Line

HEK293 reporter model for interferon-stimulated response element activity.

1 image

ISRE

Functional Assay

$2072
Cat # RC1043
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

NF-kB Luciferase Reporter-Jurkat Cell Line

Jurkat NF-kB reporter cell line for inflammation and immune signaling assays.

1 image

NF-kB

Functional Assay

$2072
Cat # RC1042
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

ISRE Luciferase Reporter-RAW264.7 Cell Line

Macrophage-like reporter model for ISRE and immune signaling studies.

1 image

ISRE

Functional Assay

$2072
Cat # RC1041
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

STAT4 Luciferase Reporter-HEK293 Cell Line

HEK293 reporter model for STAT4 cytokine signaling response.

1 image

STAT4

Functional Assay

$5040
Cat # RC1040
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

TLR5/NF-kB Luciferase Reporter-HEK293 Cell Line

HEK293 reporter model for TLR5-mediated NF-kB pathway activation.

1 image

TLR5 / NF-kB

Functional Assay

$7280
Cat # RC1039
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

ATF6 Luciferase Reporter-HeLa Cell Line

HeLa reporter model for ATF6 and cellular stress response studies.

1 image

ATF6

Functional Assay

$2072
Cat # RC1038
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

MRE Luciferase Reporter-HEK293 Cell Line

HEK293 reporter model for metal response element activity.

MRE HEK293 reporter cell line for metal response

1 image

MRE

Functional Assay

$2072
Cat # RC1037
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

SBE Luciferase Reporter-HEK293 Cell Line

HEK293 reporter model for SBE-mediated pathway analysis.

SBE HEK293 reporter cell line for pathway analysis

1 image

SBE

Functional Assay

$2072
Cat # RC1036
1 Vial, 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

Displaying Items 1-10 of Many

Browse All Reporter Cell Lines

Need a pathway, promoter, receptor, or cell background not listed?

Boster Bio can support custom reporter cell line generation for specialized assay needs, including custom response elements, receptor systems, and host cell backgrounds.

Request Custom Cell Line

Explore Available Reporter Pathways by Cell Background

Use the tabs below to review reporter-labeled pathway options across commonly used research cell backgrounds. Linked pathway names lead to Boster Bio pathway maps where available.

ISRE	STAT4	TLR/NF-kB	MRE
SBE	CRE	SRF-RE	SRE
TLR9/NF-kB	TLR8/NF-kB	TLR7/NF-kB	TLR3/NF-kB
TLR2/NF-kB	NF-kB	TLR3/ISRE	TLR3/IFNB
TCF/LEF	STAT3	NFAT	GATA3
TNF-β	TNF-α	AP-1

ISRE	iNOS	STAT1	NFAT
TNF-β	TNF-α	MIP-2	IL-8
NF-kB

ATF6	P53	TLR-4/IL-8	HRE
STAT1

MDA/NF-kB	RIG-I/NF-kB

STAT4	STAT5

FOXP3	NF-kB

Nrf2

IL-6

What Is a Luciferase Reporter Cell Line?

A luciferase reporter cell line is a stable cell model engineered to express a luciferase reporter gene under the control of a selected promoter or response element. When the target signaling pathway is activated, the reporter is expressed and produces a luminescent signal that can be measured as a quantitative readout.

Renilla luciferase reporter cell lines are commonly used to study transcriptional regulation, receptor-mediated signaling, promoter activity, and compound effects. Compared with transient transfection workflows, stable reporter cell lines can reduce setup time and improve assay consistency across screening and functional validation studies.

Why Use a Stable Reporter Cell Line?

Quantitative luminescent readout
Useful for pathway activity and promoter response studies
Suitable for activator and inhibitor screening
Reduces repeated reporter construct setup
No radioactive material handling
Supports reproducible functional assay workflows

How a Renilla Luciferase Reporter Assay Works

Renilla luciferase catalyzes a light-producing reaction with coelenterazine. In a reporter cell line, the luciferase signal reflects the activity of the promoter or response element placed upstream of the reporter gene. After cells are treated with a stimulus, inhibitor, biologic, or small molecule, luminescence is measured with a luminometer and reported as relative light units (RLU).

The result is a functional assay readout that helps researchers compare pathway activation, inhibition, and dose-response effects under controlled experimental conditions.

How Renilla luciferase reporter gene assay works

Reporter Cell Line Applications

Gene Expression Analysis

Study promoter regulation and transcriptional response through luciferase-based reporter readouts.

Pathway Activation Studies

Measure signaling pathway activation and inhibition under controlled treatment conditions.

Transcription Factor Assays

Analyze transcription factor and response-element activity in functional cell-based systems.

Compound Screening

Compare activators, inhibitors, biologics, and small molecules through quantitative luminescent readouts.

General Workflow for a Renilla Luciferase Reporter Cell Assay

Exact conditions may vary by reporter cell line and experimental objective. Always follow the product-specific datasheet and recommended culture conditions.

Choose the appropriate reporter cell line by pathway, response element, and cell background.
Thaw, recover, and culture cells according to the product-specific handling instructions.
Plate reporter cells at the recommended assay density.
Treat cells with your stimulus, inhibitor, biologic, or test compound alongside proper controls.
Incubate under optimized assay conditions, commonly around 24 to 48 hours depending on the system.
Lyse cells and add the appropriate Renilla luciferase substrate system.
Measure luminescence with a luminometer and analyze relative light units against controls.

Common Reporter Cell Backgrounds

Cell background affects pathway biology, receptor expression, growth behavior, and assay interpretation. Select the cell model that best matches your experimental question.

HEK293 & HEK293T

Widely used human cell backgrounds for pathway reporter assays, receptor signaling studies, and recombinant expression workflows. HEK293-based reporter models are often selected for robust growth and assay adaptability.

Jurkat

A commonly used T cell leukemia background for immune signaling, transcription factor response, NF-kB, FOXP3, and T cell pathway studies.

RAW264.7

A macrophage-like cell background useful for innate immune signaling, inflammatory response, ISRE, iNOS, NF-kB, and cytokine-related reporter assays.

HeLa

A human epithelial cancer cell background often used for stress response, hypoxia response, viral response, and general pathway activity studies.

Ba/F3

A hematopoietic pro-B cell background often used in cytokine, kinase, and signaling studies where growth response and pathway activation are central to the assay design.

Custom Backgrounds

When your assay requires a specific disease model, receptor system, promoter, or host cell type, request a custom reporter cell line to better match your biology.

Reporter Cell Line FAQs

Q1: What are luciferase reporter cell lines used for?

Luciferase reporter cell lines are used to measure pathway activation, promoter activity, receptor signaling, transcription factor response, and compound effects through a quantitative luminescent readout.

Q2: How do I choose the right reporter cell line?

Choose based on the pathway or response element you need to monitor, the cell background required for your biology, and whether your assay can use a catalog stable reporter cell line or requires a custom reporter model.

Q3: What is the difference between Renilla and firefly luciferase reporter systems?

Renilla and firefly luciferase systems use different enzymes and substrates. Renilla luciferase commonly uses coelenterazine-based detection, while firefly luciferase uses luciferin-based substrates. The right system depends on assay design, detection format, and whether a single- or dual-reporter workflow is required.

Q4: Can reporter cell lines be used for compound screening?

Yes. Stable reporter cell lines are commonly used to screen activators, inhibitors, biologics, and small molecules that affect pathway activity or receptor-mediated signaling.

Q5: How are Boster Bio reporter cell lines validated?

Boster Bio reporter cell lines are validated for functional assay use. Product pages may include activity validation figures, assay information, catalog format, and handling details for the specific reporter model.

Q6: How should reporter cell lines be handled upon arrival?

Reporter cell lines are typically shipped frozen and should be handled according to the product-specific instructions. Review the datasheet before thawing, avoid unnecessary freeze-thaw cycles, and follow the recommended culture conditions for recovery and propagation.

Q7: Does Boster offer custom reporter cell lines?

Yes. If a catalog reporter cell line does not match your required pathway, promoter, receptor, response element, or cell background, Boster Bio can support custom reporter cell line generation.