REPORTER CELL LINES WITH RENILLA LUCIFERASE

Boster Bio offers luciferase reporter cell lines for cell-based reporter assays. These cell lines are stably transfected common cell lines which express the Renilla luciferase reporter gene under the transcriptional control of popular transcription related genes. These cell lines are designed to measure the transcriptional activity of the target transcription genes.

Browse Reporter Cell Lines

Browse reporter cell lines with renilla luciferase

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Functional Assay
$4785
Cat # RC1045
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

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Functional Assay
$2003
Cat # RC1044
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

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Functional Assay
$2003
Cat # RC1043
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1042
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1041
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$4785
Cat # RC1040
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$6885
Cat # RC1039
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1038
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1037
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1036
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

Displaying Items 1-10 of Many

What is a reporter cell line with Renilla luciferase?

Reporter cell lines are common stable cell lines that have been labelled with a reporter gene, such as Renilla luciferase. The Renilla luciferase reporter cell line is a stably transfected cell line which expresses the Renilla luciferase reporter gene under the transcriptional control of a selected promoter. The reporter allows for the monitoring and visualization of specific proteins and their expression. Renilla luciferase reporter cell lines measure transcriptional activity with bioluminescence.

Reporter cell lines are stably transfected common cell lines that allow for highly sensitive quantification of engrafted target cells. Researchers utilize reporter gene assays to study signaling pathways, gene regulation, and structure of regulatory elements. These reporter genes monitor receptor-mediated cellular processes at the transcriptional level.

Benefits of using a reporter cell line with Renilla luciferase

  • Exceptional quantitation for mammalian cells
  • Tight coupling of protein synthesis with enzyme activity
  • Rapid and reproducible results
  • Easy to perform assay
  • No radioactive material handling
  • Linear range of activity >5 orders of magnitude

What is Renilla luciferase

Renilla luciferase is an enzyme from the shallow-water soft coral Renilla reniformis, commonly known as the sea pansy. Renilla’s molecular biology and chemistry is well-studied. The monomeric, 36 kDa protein catalyzes a chemical reaction between coelenterate and luciferin (coelenterazine) responsible for its bioluminescence. The resulting photon emission is a result of the oxidation of coelenterazine to produce light. Renilla luciferase is a powerful monitoring system for biological processes, as post-translational modification is unnecessary for activity.

How Does A Renilla Luciferase Reporter Gene Assay Work?

A Renilla luciferase reporter gene assay, utilizes the luciferase enzyme and the substrate coelenterazine to study gene regulation at the transcriptional level.

The Renilla luciferase reporter gene assay monitors your promoter of choice induction activity by screening of the promoter’s signaling pathway for activators and inhibitors. The assay allows exploration of a protein’s ability to activate or inhibit the expression of a target gene. Using Renilla luciferase as a reporter protein results in a chemical reaction between the enzyme and a coelenterazine substrate, resulting in the emission of photons - bioluminescence. This bioluminescence is quantified as a direct measure of its enzymatic activity.

luciferase-reporter-gene-assay

RENILLA LUCIFERASE REPORTER
CELL LINES Applications

Gene expression and regulation analysis

Signal pathway mapping

Promoter and regulatory element structure analysis

Protein folding and protein-protein interactions

SNP (single nucleotide polymorphism) analysis

Antiviral research and therapy

Cytotoxicity assay

Drug discovery

General protocol for Renilla
luciferase reporter cell assay

This protocol may vary per application and experimental objective, but should be similar for either a single reporter or dual reporter assay (dual reporter assays typically use Renilla luciferase as an internal control to normalize experimental variations arising from sample handling and transfection efficiency)

  • Choose appropriate Renilla luciferase reporter cell line

    *In a dual reporter assay to choose luciferases that have differed spectral measurements.

  • Clone the luciferase into your plasmid

    *In a dual reporter assay the other luciferase will be cloned into a separate plasmid

  • Co-transfect the experimental cells with your plasmid

  • Incubate for ~24 to 48 hours

  • Remove media and lyse cells

  • Add coelenterazine buffer to lysate

    *Different substrate buffers, such as luciferin, are used for non-Renilla species

  • Measure bioluminescence with luminometer

FAQs

When preforming a reporter cell assay with Renilla luciferase, only use ingredients that are compatible with bioluminescent reagents. This will protect the reagents signal sensitivity.

Cell lysates are measured in either multi-well plates or tubes with a luminometer. Luminometers measure the photon emission produced from the luciferase reaction. Emissions are reported in relative light units (RLU). The addition of an injector enables measurements immediately, minimizing diminishing signal.

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