Boster Bio Reporter-Labeled Cell Lines

Reporter Cell Lines with Renilla Luciferase

Boster Bio offers luciferase reporter cell lines for cell-based reporter assays.

  1. Ready-to-use validated cell lines
  2. Get real-time data
  3. Custom cell lines available
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Get real-time data with Boster Bio’s ready-to-use validated reporter cell lines with Renilla luciferase.

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Browse reporter cell lines with renilla luciferase

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Functional Assay
$4785
Cat # RC1045
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

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Functional Assay
$2003
Cat # RC1044
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1043
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1042
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1041
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$4785
Cat # RC1040
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$6885
Cat # RC1039
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1038
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1037
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

1 images

Functional Assay
$2003
Cat # RC1036
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.

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What is a reporter cell line with Renilla luciferase?

Reporter cell lines are common stable cell lines that have been labelled with a reporter gene, such as Renilla luciferase. The Renilla luciferase reporter cell line is a stably transfected cell line which expresses the Renilla luciferase reporter gene under the transcriptional control of a selected promoter. The reporter allows for the monitoring and visualization of specific proteins and their expression. Renilla luciferase reporter cell lines measure transcriptional activity with bioluminescence.

Reporter cell lines are stably transfected common cell lines that allow for highly sensitive quantification of engrafted target cells. Researchers utilize reporter gene assays to study signaling pathways, gene regulation, and structure of regulatory elements. These reporter genes monitor receptor-mediated cellular processes at the transcriptional level.

Benefits of using a reporter cell line with Renilla luciferase

  • Exceptional quantitation for mammalian cells
  • Tight coupling of protein synthesis with enzyme activity
  • Rapid and reproducible results
  • Easy to perform assay
  • No radioactive material handling
  • Linear range of activity >5 orders of magnitude

What is Renilla Luciferase

Renilla luciferase is an enzyme from the shallow-water soft coral Renilla reniformis, commonly known as the sea pansy. Renilla’s molecular biology and chemistry is well-studied. The monomeric, 36 kDa protein catalyzes a chemical reaction between coelenterate and luciferin (coelenterazine) responsible for its bioluminescence. The resulting photon emission is a result of the oxidation of coelenterazine to produce light. Renilla luciferase is a powerful monitoring system for biological processes, as post-translational modification is unnecessary for activity.

what-is-renilla-luciferase

How Does A Renilla Luciferase Reporter Gene Assay Work?

A Renilla luciferase reporter gene assay, utilizes the luciferase enzyme and the substrate coelenterazine to study gene regulation at the transcriptional level.

The Renilla luciferase reporter gene assay monitors your promoter of choice induction activity by screening of the promoter’s signaling pathway for activators and inhibitors. The assay allows exploration of a protein’s ability to activate or inhibit the expression of a target gene. Using Renilla luciferase as a reporter protein results in a chemical reaction between the enzyme and a coelenterazine substrate, resulting in the emission of photons - bioluminescence. This bioluminescence is quantified as a direct measure of its enzymatic activity.

how-renilla-luciferase-reporter-gene-assay-works

RENILLA LUCIFERASE REPORTER
CELL LINES Applications

Gene expression and regulation analysis

Signal pathway mapping

Promoter and regulatory element structure analysis

Protein folding and protein-protein interactions

SNP (single nucleotide polymorphism) analysis

Antiviral research and therapy

Cytotoxicity assay

Drug discovery

General protocol for Renilla
luciferase reporter cell assay

This protocol may vary per application and experimental objective, but should be similar for either a single reporter or dual reporter assay (dual reporter assays typically use Renilla luciferase as an internal control to normalize experimental variations arising from sample handling and transfection efficiency)

  • Choose appropriate Renilla luciferase reporter cell line

    *In a dual reporter assay to choose luciferases that have differed spectral measurements.

  • Clone the luciferase into your plasmid

    *In a dual reporter assay the other luciferase will be cloned into a separate plasmid

  • Co-transfect the experimental cells with your plasmid

  • Incubate for ~24 to 48 hours

  • Remove media and lyse cells

  • Add coelenterazine buffer to lysate

    *Different substrate buffers, such as luciferin, are used for non-Renilla species

  • Measure bioluminescence with luminometer

Pick Your Signaling Pathways

ISRE STAT4 TLR/NF-KB MRE
SBE CRE SRF-RE SRE
TLR9/NF-KB TLR8/NF-KB TLR7/NF-KB TLR3/NF-KB
TLR2/NF-KB NF-KB TLR3/ISRE TLR3/INFB
TCF/LEF STAT3 NFAT GATA3
TNF-β TNF-α AP-1
ISRE INOS STAT1 NFAT
TNF-β TNF-α MIP-2 IL-8
NF-KB
ATF6 P53 TLR-4/IL-8 HRE
STAT1
MDA/NF-KB RIG-I/NF-KB
STAT4 STAT5
FOXP3 NF-KB
Nrf2
IL-6

Which Cell Lines Have Reporter-Labeled Pathways?

HeLa Cell Lines

Be Aware (& practice good aseptic technique)! Although possible with other cell types, HeLa cells are so notoriously prolific that countless studies over the years have been challenged after it was found that HeLa cells could float on dust particles in the air and contaminate other cell cultures. Good authentication and validation practices will additionally help detect any cross-contamination with other cell lines.

HeLa Cell Lines

HeLa cell lines are the first immortalized and most widely used human cell line. Obtained from a cervical tumor in 1951 during Henrietta Lack’s treatment of cancer at Johns Hopkins, the highly prolific HeLa cell line continues to make a wide-range of diverse contributions towards many different fields of research and medical discovery. For example, HeLa cell lines contributed significantly towards the development of the COVID-19, polio, tetanus, and many other vaccines. HeLa cells are also used for research of cancer and underlying disease mechanisms, AIDS, viral prevalence to cervical cancers (like HPV), the effects of radiation exposure, to test human sensitivity to certain drugs and chemicals, and many more significant discoveries.

The distinct difference between HeLa cells and normal human cells that directly contribute to HeLa’s prolific success:

  • HeLa cell are cancer cells. And not only that, HeLa cells grow fast. Faster than other cancer cells - by a lot - making them ideal for both research and upstream large scale projects.

Doubling time ≈ 34 hours

HEK293 & HEK293T Cell Lines

HEK293 & HEK293T Cell Lines

Human embryotic kidney (HEK293) cell lines are an extensively used immortalized cell line grown in tissue culture. The original HEK cell line was derived by transformation with sheared Adenovirus 5 DNA. After repeated attempts to cultivate, a rapidly growing, single transformed clone was isolated and dubbed as HEK293. Transfection with adenovirus genes results in a highly prolific model system.

HEK293T cells are a daughter cell line derived from the original HEK293 cells and transfected with a SV40 plasmid vector. The SV40 transfection allows for an even higher production of recombinant proteins.

Doubling time ≈ 33 hours

BaF3 Cell Lines

BaF3 Cell Lines

The Ba/F3 cell line derives from the murine C3H species as a hematopoietic, immortalized pro-B cell line, dependent on IL3 for growth. Ba/F3 cells provide a model system as powerful indicators of kinase activity, such as potency, mutations and downstream signaling, or inhibition of kinase activity.

Protein kinases are popular targets for drug discovery as they have the ability to behave as dominant oncogenes across a wide variety of cancers.

Doubling time ≈ 20 hours

Jurkat Cell Lines

Jurkat Cell Lines

Jurkat cell lines are immortalized T-lymphocyte cell lines. They are typically utilized as a model system to study T-cell activity, such as T-cell signaling, acute T-cell leukemia, and chemokine receptor expression in the infection cycle of HIV, specifically latency and activation. Jurkat cell lines are extensively used to model T-cell activation and map the critical underlying pathways.

Doubling time ≈ 26 hours (with some subclones doubling in considerably shorter timeframe of 16 to 20 hours)

RAW264.7 Cell Lines

RAW264.7 Cell Lines

RAW264.7 cell lines are one of the most commonly used murine myeloid, immortalized cell lines. The monocyte/macrophage-like cells are derived from BALB/c mice transformed with Abelson leukemia virus. Their ability to perform pinocytosis and phagocytosis make them a robust macrophage model. RAW264.7 cell lines are used extensively to study the internalization and intercellular survival of microbes, osteoclastogenic properties of bones disease, and compounds effective at reducing pro-inflammatory cytokines.

Doubling time ≈ 12 hours

MCF7 Cell Lines

MCF7 Cell Lines

MCF7 cell lines originate from a female human adenocarcinoma breast cancer cell line. As the first hormone responding breast cancer cell line, this immortalized and non-transformed epithelial cell line possesses receptors for estrogen, progesterone, and glucocorticoid, and maintains many characteristics of differentiated mammary epithelium.

The MCF7 cells are used to study the mechanisms of carcinogenesis and as a model for breast cancer treatment. The cells are also used to screen potential anticancer drugs and as an in vitro system for testing the efficacy of new drugs.

Doubling time ≈ 30 – 40 hours

NIN 3T3 Cell Lines

NIN 3T3 Cell Lines

NIN 3T3 is a murine fibroblast immortalized cell line rich in proteins. They are used for the production of polyclonal antibodies, as a source of antigen to generate monoclonal antibodies, and as a source of tumor cells for in vitro studies. They are used in the study of B-cell differentiation, immunoglobulin production, and also to study how viruses affect cell growth and how they react to different treatments.

Doubling time ≈ 20 – 26 hours

FAQs

The Renilla luciferase reporter cell line is a stably transfected cell line which expresses the Renilla luciferase reporter gene under the transcriptional control of a promoter. The reporter allows for the monitoring and visualization of specific protein and transcription factor expression. If a specific signaling pathway is activated, the luciferase is transcribed and the reporter is expressed as luminescence (light). Renilla luciferase reporter cell lines measure transcriptional activity in real-time with bioluminescence.

Boster Bio’s proprietary reporter cell lines are extensively validated for functional assay of small molecules. Our innovative technology delivers increased sensitivity with a prolonged signal intensity. Each cell line includes documentation of activity validation.

It is recommended to thaw cells immediately upon receipt. Cells are shipped on dry ice (not as cold as liquid nitrogen) therefore thawing has begun in shipping. Immediately storing at -80 °C or on liquid nitrogen will cause cell damage thus decreasing cell viability. The thaw cycle is a crucial beginning step for successful propagation. Do not refreeze reporter cells once thawed.

A typical reporter cell line will require you to thaw frozen cells immediately upon receipt from frozen or liquid nitrogen in water bath at 37 °C.

Be sure to check the culture conditions included with your cell lines, or under the product listing on our website.

Yes, you’ll just need to make a standard curve using a positive control. Here’s some help preparing standards.

Yes, Boster Bio’s reporter cell lines may be used for other assays. Please be aware that they are not sold for this purpose.

Cell lysates are measured in either multi-well plates or tubes with a luminometer. Luminometers measure the photon emission produced from the luciferase reaction. Emissions are reported in relative light units (RLU). The addition of an injector enables measurements immediately, minimizing diminishing signal and experimental variability, such as pipetting errors.

The background levels of reagent without Renilla luciferase is extremely low, resulting in total luminescence being directly proportional to luciferase activity. That being said, it is almost always necessary to take a background reading. This ensures there is no signal from your instrument, sample tubes/plates, or any autoluminescence directly from the Renilla luciferase. Coelenterazine may nonenzymatically oxidize in solution.

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