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Anti-PD-L1/CD274 Antibody Picoband™
Rabbit IgG polyclonal antibody for Programmed cell death 1 ligand 1(CD274) detection. Tested with WB in Human.
Anti-PD-L1/CD274 Antibody Picoband™ Info At A Glance
| SKU: | PB9154 |
|---|---|
| Size: | 100μg/vial |
| Reactivity: | Human |
| Host: | Rabbit |
| Applications: | WB |
Product Features:
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
Overview
| Product Name | Anti-PD-L1/CD274 Antibody Picoband™
See all PD-L1 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | PB9154 |
| Pack Size | 100μg/vial |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description | Polyclonal antibody for PD L1/CD274 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. PD L1/CD274 information: Molecular Weight: 33275 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
| Cite This Product | Anti-PD-L1/CD274 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9154) |
| Similar Products From Other Companies |
Anti-PD-L1/CD274 Antibody Picoband™ may replace the following items: sc 19090|sc 19091|sc 50298. |
Product Specs
| Host | Rabbit |
|---|---|
| Reactivity/Species | Human |
| Applications | WB
*Our Boster Guarantee covers the use of this product in the above tested applications. *Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information. **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen | E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. |
| Cross Reactivity | No cross reactivity with other proteins |
Gene/Protein Basic Information For CD274 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9NZQ7 |
|---|---|
| NCBI Gene Id | 29126 |
| Species Of This Entry | Human |
| Gene Name | CD274 |
| Protein Name | Programmed cell death 1 ligand 1 |
| Superfamily | immunoglobulin superfamily |
| Alternative Names | PD-L1|B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 |
| Molecular Weight | 33275 |
| Post Tranlational Modifications | Phosphorylation; Reduction; Cleavage; Glycosylation; Dephosphorylation |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Endomembrane system; Single-pass type I membrane protein. |
| Gene Location | 9p24.1, on Chromasome 9, gene sequence: NC_000009.12 (5450503..5470567) |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CD274 (Source: Uniprot.org, NCBI)
| Protein Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). |
|---|---|
| Tissue Specificity | Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
| Interacting Proteins | CD80; CMTM4; CMTM6; PDCD1 |
| Research Areas | Signal Transduction |
| Related Diseases | Neoplasms; Malignant Neoplasms; Autoimmune Reaction; Carcinoma; Virus Diseases; Inflammation; Infective Disorder; Autoimmune Diseases; Tumor Escape; Hepatitis; Decreased Immunologic Activity [pe]; Exhaustion; Diabetes Mellitus; Melanoma; Autoimmunity; Hepatitis B; Malignant Paraganglionic Neoplasm; Hiv Infections; Hepatitis B, Chronic; Hepatitis, Chronic |
| Related Pathways | Cytokine Production; Immune Response; Cell Activation; T Cell Activation; Cell Death; Cell Proliferation; Secretion; Programmed Cell Death; T Cell Proliferation; Pathogenesis; Inflammatory Response; Cytokine Secretion; Sensitization; Hypersensitivity; Tolerance Induction; Lymphocyte Proliferation; Localization; Lymphocyte Activation; Virulence; Cell Migration |
| Background | Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer. |
| Scientific References | PMID: 10581077 by Dong H., et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. PMID: 11015443 by Freeman G.J., et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation. |
Our Boster Quality Guarantee for Anti-PD-L1/CD274 Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-PD-L1 Picoband antibody, PB9154-1.jpg
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: Recombinant Human PDL1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: Recombinant Human PDL1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-PD-L1 Picoband antibody, PB9154-2.jpg
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
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Questions and answers from customers related to PB9154 Anti-PD-L1/CD274 Antibody Picoband™
12 Related Questions
Question
Please suggest the suitable negative control tissue for your product Anti-PD-L1/CD274 Antibody Picoband for IHC applications.
Verified Customer
Asked: 2018-07-26
Answer
PD-L1 expressions varies by tumor type so screening is recommended to find positive and negative tumor controls. You can use endothelial cells as negative internal controls within a positive (tumor-) tissue but remember that many tumor specimens have some inflammatory macrophages and tumor associated immune cells which are expected to be positive. That
Boster Scientific Support
Answered: 2018-07-26
Question
My colleagues were content with the WB result of your anti-PD-L1/CD274 antibody. However we have observed positive staining in gastric carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CD274 supposed to be expressed?
Verified Customer
Asked: 2020-04-28
Answer
According to literature, gastric carcinoma does express CD274. Generally CD274 expresses in cell membrane. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-PD-L1/CD274 antibody PB9154 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Asked: 2020-01-08
Answer
The anti-PD-L1/CD274 antibody (PB9154) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Here is the WB image, lot number and protocol we used for liver using anti-PD-L1/CD274 antibody PB9154. Please let me know if you require anything else.
Verified Customer
Asked: 2019-09-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
Is this PB9154 anti-PD-L1/CD274 antibody reactive to the isotypes of CD274?
Verified Customer
Asked: 2019-06-18
Answer
The immunogen of PB9154 anti-PD-L1/CD274 antibody is E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-18
Question
Does anti-PD-L1/CD274 antibody PB9154 work for WB with liver?
Verified Customer
Asked: 2018-05-21
Answer
According to the expression profile of liver, CD274 is highly expressed in liver. So, it is likely that anti-PD-L1/CD274 antibody PB9154 will work for WB with liver.
Boster Scientific Support
Answered: 2018-05-21
Question
We are interested in to test anti-PD-L1/CD274 antibody PB9154 on human liver for research purposes, then I may be interested in using anti-PD-L1/CD274 antibody PB9154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Williams
Asked: 2018-02-26
Answer
The products we sell, including anti-PD-L1/CD274 antibody PB9154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PD-L1/CD274 antibody PB9154. Let me know if you need anything else.
Verified Customer
Asked: 2018-01-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-24
Question
Does anti-PD-L1/CD274 antibody PB9154 work on zebrafish WB with liver?
Verified Customer
Asked: 2017-11-21
Answer
Our lab technicians have not validated anti-PD-L1/CD274 antibody PB9154 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PD-L1/CD274 antibody PB9154 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-21
Question
I was wanting to use your anti-PD-L1/CD274 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Asked: 2017-05-23
Answer
You can see on the product datasheet, PB9154 anti-PD-L1/CD274 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-23
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-PD-L1/CD274 antibody supposed to stain liver positively?
M. Miller
Asked: 2015-05-22
Answer
Based on literature liver does express CD274. Based on Uniprot.org, CD274 is expressed in chorionic villus, placenta, gastric carcinoma, lung, liver, among other tissues. Regarding which tissues have CD274 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 11015443, 14702039
Boster Scientific Support
Answered: 2015-05-22
Question
My question regards using your anti-PD-L1/CD274 antibody for positive regulation of t cell proliferation studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
C. Banerjee
Asked: 2015-01-22
Answer
We appreciate your inquiry. This PB9154 anti-PD-L1/CD274 antibody is tested on hepg2 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-22
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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PB9154
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00
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