Overview
| Product Name |
Anti-PD-L1/CD274 Antibody Picoband™
See more information about PD-L1, and browse related primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
PB9154 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for PD L1/CD274 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. PD L1/CD274 information: Molecular Weight: 33275 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
| Cite This Product |
Anti-PD-L1/CD274 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9154)
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| Antibodies Validation |
Antibodies Validation Information
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Similar Products From
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Anti-PD-L1/CD274 Antibody Picoband™ may replace the following items: sc 19090|sc 19091|sc 50298. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For CD274 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9NZQ7 |
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| NCBI Gene Id | 29126 |
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| Species Of This Entry | Human |
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| Gene Name | CD274 |
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| Protein Name | Programmed cell death 1 ligand 1 |
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| Superfamily | immunoglobulin superfamily |
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| Alternative Names | PD-L1|B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 |
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| Molecular Weight | 33275 |
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| Post Tranlational Modifications | Phosphorylation; Reduction; Cleavage; Glycosylation; Dephosphorylation |
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| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Endomembrane system; Single-pass type I membrane protein. |
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| Gene Location | 9p24.1, on Chromasome 9, gene sequence: NC_000009.12 (5450503..5470567) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CD274 (Source: Uniprot.org, NCBI)
| Protein Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077). |
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| Tissue Specificity | Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. |
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| Interacting Proteins | CD80; CMTM4; CMTM6; PDCD1 |
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| Research Areas | Signal Transduction |
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| Related Diseases | Neoplasms; Malignant Neoplasms; Autoimmune Reaction; Carcinoma; Virus Diseases; Inflammation; Infective Disorder; Autoimmune Diseases; Tumor Escape; Hepatitis; Decreased Immunologic Activity [pe]; Exhaustion; Diabetes Mellitus; Melanoma; Autoimmunity; Hepatitis B; Malignant Paraganglionic Neoplasm; Hiv Infections; Hepatitis B, Chronic; Hepatitis, Chronic |
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| Related Pathways | Cytokine Production; Immune Response; Cell Activation; T Cell Activation; Cell Death; Cell Proliferation; Secretion; Programmed Cell Death; T Cell Proliferation; Pathogenesis; Inflammatory Response; Cytokine Secretion; Sensitization; Hypersensitivity; Tolerance Induction; Lymphocyte Proliferation; Localization; Lymphocyte Activation; Virulence; Cell Migration |
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| Background | Programmed death-ligand 1 (PD-L1) also known as CD274 or B7-H1 is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, It has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer. |
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| Scientific References | PMID: 10581077 by Dong H., et al. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.
PMID: 11015443 by Freeman G.J., et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation. |
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Our Boster Quality Guarantee for Anti-PD-L1/CD274 Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-PD-L1 Picoband antibody, PB9154-1.jpg
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: Recombinant Human PDL1 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-PD-L1 Picoband antibody, PB9154-2.jpg
All lanes: Anti PDL1 (PB9154) at 0.5ug/ml
WB: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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