Loading control antibodies

Purpose of loading controls: Loading control antibodies react with proteins that are ubiquitously and constitutively expressed. In Western blotting, loading controls play many roles, such as indicating the total amount of protein loaded in each lane, normalizing protein levels in quantitative WB, and act as positive control to show the WB assay and reagents used are working properly.

Browse Loading Control Antibodies

Browse loading control antibodies

Product
Figures
Species
Applications
Price

1 images

Human, Mouse, Rat
ELISA, WB
$315
Cat # A01843-1
100 μg/vial

22 images

Human
IHC, WB
$494
Cat # M02177-1
100 ul

4 images

Human, Mouse, Rat
Flow Cytometry, IF, IHC-P, WB
$166
Cat # M01280-4
50 ul

4 images

Human
Flow Cytometry, IF, IHC-P, WB
$170
Cat # A00325-2
80 ul

4 images

Dog, Human, Monkey, Mouse, Rat
Flow Cytometry, IF, WB
$494
Cat # M00256-1
100 ul

13 images

Human, Monkey
Flow Cytometry, IHC, WB
$494
Cat # M00595-1
100 ul

10 images

Dog, Human, Monkey, Rat
Flow Cytometry, IF, IHC, WB
$524
Cat # M00595-2
100 ul

6 images

Human
Flow Cytometry, IHC, WB
$494
Cat # M05215-2
100 ul

7 images

Human
IF, IHC, WB
$494
Cat # M05215-1
100 ul

4 images

Human, Mouse, Rat
Flow Cytometry, IHC-P, WB
$166
Cat # M03229-3
50 ul

Displaying Items 1-10 of Many

how to choose loading control for western blot

There are 3 things to consider when designing your WB with loading control.

  1. Protein size: the loading control needs to have a sufficient size difference to your target protein. If the size are too close the bands will mush together data interpretation will be difficult. That said, the sizes should not be too different either.
  2. Protein level: the loading control should have similar abundance to target protein. If too different, in the same development time, the more abundant protein will over develop while the less abundant protein will show weak or no signal.
  3. Orthogonality: even though loading control proteins are typically ubiquitously and constitutively expressed, this is not absolute. Some conditions might affect these proteins still, and it is important to know and avoid using these affected proteins' antibodies as loading controls.

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