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WESTERN BLOT SERVICE

Considering outsourcing your WB experiments? Let us help you take control over your Western blots. As a dear customer puts it, "trade money for time", with our fast turnaround Western blot service.

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Affordable WB Service with Fast Turnaround

Send us your samples and receive data and analysis. Boster Bio is an antibody manufacturer and runs hundreds of WB every week for our internal production quality control. Our team members have 3 decades of WB know-how and a streamlined WB workflow. That's why we can do your blots at affordable prices with high quality AND fast turnaround time.

We are also a contributor of WB knowledge on the internet with thousands of visitors to our free WB resources each month.

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Factors to consider for outsourcing

Increasingly more labs are outsourcing their experiments. This trend has accelerated as more labs fall behind their schedules due to the COVID-19 pandemic. Below we lay out the pros and cons of outsourcing experiments, and list a few factors to look for when outsourcing Western blotting.

Pros

  • More cost-effective:

    While in-house WB may require significant investment (e.g. equipment cost and maintenance, labor), a specialized lab can perform western blot more cost efficiently. With WB being taken care of by a trusted lab, you can allocate your budget and resources for use on other projects or programs.

    In some cases, you can outsource to other countries to save cost, such as screening/hunting for antibodies that work. Boster also provides such service--see details of our antibody hunting service here. We also receive discounts from our partnering antibody companies and can pass on the savings to you.

  • Experts perform the testing:

    Working with a specialized lab enables access to expertise, more resources, and advanced technologies. The CRO lab will be capable of performing successful western blot experiments in a high-throughput manner while avoiding common WB technical issues, such as nonspecific bands, weak/no signal, saturated protein detection, high background, etc., through various optimizations (i.e. protein isolation, buffers, type of separation, gel concentration, etc.).

  • More time to focus on core goals:

    Success comes from focus. The 80/20 principle (Pareto's Principle) is true for Western blotting as it is for life--the key to success is freeing up your bandwidth to focus on essential objectives. By outsourcing your WB experiments, you’ll have the flexibility to devote more time, energy, and resources on other strategically important initiatives and accelerate your timelines.

Cons

  • Less flexibility and control: When it comes to innovative, non-standard techniques, the vendor may have limited capacity to accommodate.
  • Chance of miscommunication: Miscommunications can happen with your in-house team too, but miscommunications with your outsourcing vendor are harder to notice. The responsiveness of the service vendor is crucial for avoiding and spotting miscommunication quickly.
  • Risk in sending samples: Biological samples are fragile. If you have never shipped your samples to someone, it is best to send some low value samples first to work out all the kinks before sending the samples you spent 6 months to obtain.
  • Loss of educational value: Many labs also bear the obligation to educate. When you outsource your work, the new PhD student would get his/her data faster, but might miss the chance of becoming a Western blot ninja.

Western blot made easy

Outsource WB experiments to us if you:

  1. Want to free your lab from running gels and transfers.
  2. Are bothered by the thought of buying and testing yet one more antibody.
  3. Don't have time to run all samples.
  4. Need faster turnaround times while obtaining publishable data.
  5. Want a CRO as your long-term partner to broaden the bandwidth of your lab/team in a flexible manner.

BOSTER WB SERVICE VS. ALTERNATIVES

Boster Bio Western Blotting Service

Cost ++ Our cost is on the lower side
Turnaround +++ Typically < 1 week
Service Level +++ Personal attention is our creed
Special Needs ++ We try our best
Logistics ++ Ship samples overnight
Credential +++ 28+ years manufacturing antibodies

Boster Bio WB service Advantages overview

As an antibody manufacturer, we are in a unique position to serve you as a WB CRO.

  1. We can do WB at a very low cost due to economies of scale--we do hundreds of them each week, and we make and sell the related chemicals and reagents. We know WB inside out after spending almost 3 decades doing dozens of blots every day.
  2. With our experience, we can offer quality and consistency service at every stage of your project.
  3. We put a lot of emphasis on responsive service communications. Our sales, support, and technical staff will try their best to accomplish your goals. They will gladly pick up the phone when you need their help.
  4. We receive discounts from collaborations with other antibody companies, enabling us to pass on the savings to you.
vs.

do it in-house

Cost ??? In-house costs are hard to calculate
Turnaround +++ 2-3 days
Service Level -- Not applicable
Special Needs +++ Your needs are not special to you
Logistics + 2 days of hard work in the lab
Credential -- Not applicable

Doing it in-house

If you are not doing it already, it might not be a good idea to start now. WB is a laborious process and tons of scientists can do it, so adding it to your lab's arsenal will not add much to your competitive advantage. If it is not core to your lab's success, outsourcing it is worth considering.

If your lab is doing it already, but considering outsourcing it, we recommend you get a quote from us today, and see if the numbers work out.

Get A Quote/Consultation


other vendors

Cost ???
Turnaround ???
Service Level ???
Special Accommodation ???
Logistics ???
Credential ???

Compare against other vendors

Since we do not know who we are going up against, we have no comments here. We hope you find the list of things to look out for useful in deciding who to go with.

If you ever need help sorting through the options, we are happy to help. Book an appointment today!

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What is Included in the Service?

Select Antibody

Tell us which commercial antibody you would like to use for WB. You can also discuss with us to determine which antibodies would work for your project.

Ship Your Samples

Ship your samples with 3-5 lbs dry ice to our facility. You can also select samples for your project from Boster Bio's range of normal and disease samples.

Get Results

In about 1 week, we will deliver a detailed report that includes the Western Blot results as well as the equipment, reagents, and protocol used.

How do I get started?

Let our team of experts provide you publishable data and optimized Western Blot protocols. For any inquires or questions, contact [email protected] and a representative from Boster will directly work with you to ensure your project is conducted as per your requirements.

You Provide
  • Purified protein (>50 ug/sample, concentration: >1 mg/ml) OR lysate sample (total protein >500 ug/sample, concentration: >1 mg/ml)
  • Primary antibody to detect target protein (or purchase from us)
  • Non-purified samples will require housekeeping proteins to normalize against
  • Total protein can be measured using biotin labeling agent and strep-HRP
  • It is recommended to use a sample expressing the target protein as the positive control
We Deliver
  • Free consultation for your project
  • Customized project report with publication-grade Western Blot exposure data image for each gel we run for you. Each gel can have 10-15 lanes. The report will also provide the equipment, reagents, and assay protocol that our team used for your project.
Turnaround About 1 week. Actual times may vary based on how many projects our team is handling.

Questions?

If you have questions about our service, feel free to send us a message. We will respond to you as soon as possible.

Western Blot Service Workflow

  • Sample Selection

    If you do not have samples, Boster has normal and disease samples available.

    User-provided sample:
    Purified protein (>50 ug/sample, >1 mg/ml)
    Lysate sample (Total protein >500 ug/sample, >1 mg/ml)

    Inquire about available samples
  • Antibody Selection

    We can use any commercially available antibody for your project. If you need assistance for selecting antibodies, we can help you.

    Inquire about antibodies
  • Optimization

    Commercial antibodies are not always validated for every sample type. You may need to optimize the dilution for your project. Typical optimization is 3-5 serial dilutions to find the optimal performance.

    If you prefer, we can also work with a dilution ratio that you have already optimized.

  • Controls

    To ensure your antibody is detecting the proper protein, it is recommended to use a sample known to express the target protein as a control.

    Boster has available positive and negative controls for your assay. We can also work with your controls.

    Inquire about controls
  • Advanced Protein Characterization

    Boster Bio provides free consultation to optimize your WB strategy. Western Blot is sometimes not sufficient for characterizing protein of interest from contaminants. We can design custom strategies such as using silver stain and LC-MS to identify contaminants.

    Contact us

Get a Free Consultation

Send us a message and one of our product managers will contact you to discuss your project.

Sample Data

Project Scope

Activate the Target X pathway by stimulation and evaluate the inhibitory effects of various treatments. Assess the level of phospho-Target X by western blot.

Conclusion

Phosphorylation of Target X was induced by Stimulant-X stimulation. The Stimulant-X-induced phosphorylation was inhibited by Control Inhibitor but not the negative control antibody. Target X phosphorylation was also inhibited by all the testing antibodies, except for Test Inhibitor 4, at 1ug/ml. The inhibitory effect reduces as the antibody concentration decreases.

Sample Report

Project Scope

Our lab performed a western blot analysis of Histone H3 using anti-Histone H3 Antibody Picoband™ (A12477-2) in 10 different samples. A specific band was detected for Histone H3 at approximately 17KD. The expected band size for Histone H3 is at 17KD. Below is a sample WB service report.

More Western blotting images

Some western blot images generated by Boster Bio's WB team.

Get Started Now

Fill out our Contact Us form and we will prepare a quote for your project.
Please contact our support team for any additional questions you have.

FAQs

Protein expression in cells has been an important cornerstone in biomedical research and industrial settings for decades. It plays a significant role in the production of therapeutic protein, monoclonal antibodies, and in the studies of cell signaling, gene regulation, protein-protein interactions, etc.

Representing at least 8-9% of all protein-related publications within the last decade, Western Blotting is estimated to be the most used technique across a broad range of scientific and clinical disciplines. Their ability to confirm the identity/presence of a specific protein both by size and through the binding of an antibody provides additional information not readily gathered from other key immunological laboratory techniques. Their results are less ambiguous, making them suitable as a key supplemental screen to be used in combination or provide confirmation of results from other techniques (ELISA, IHC, etc.)

Western blotting is informative for various instances, such as:
  • Evaluating levels of protein expression in cells
  • Monitoring fractions during protein purification
  • Comparing expression of a target protein from various tissues
  • Determining how a particular protein responds to disease or drug treatment
  • Determining the effect of time on a protein

The data produced with a Western Blot is typically considered to be qualitative and sometimes semi-quantitative, as it provides a relative comparison of protein levels, but not an absolute measure of quantity.

Both Western Blot and ELISA are two very different methods based on immunodetection. The main differences are as follows:
ELISA Western Blot
Detects the presence of antigen and antibody in cell lysates, serum, etc. Detects the presence of a specific protein and its MW from a mixture of proteins
Less time-consuming Time-consuming, delicate process, costly
Qualitative and quantitative Qualitative and sometimes semi-quantitative

Higher specificity and less likely to give false positive results

Western blotting is a technique that separates proteins by size. The smaller the protein is, the faster it migrates through the gel. However, the migration speed is also affected by other factors, so the actual band size observed may differ from what was predicted.
Possible Reasons Notes
Post-translational modifications Increases the protein size (phosphorylation, glycosylation, etc.)
Post-translational cleavage Most proteins are synthesized as pro-proteins and then cleaved to give the protein its active form (e.g pro-caspases)
Splice variants Highly variable depending on the specific tissue and experimental conditions, which may lead to the production of different sized protein products
Isoforms Many proteins express multiple isoforms that are different sizes. Expression of different isoforms for the same target protein is highly variable depending on the specific tissue and experimental conditions. Several websites can be accessed to determine isoforms for specific targets.
Relative charge The composition of amino acids (charged vs. non-charged)
Multimers An example is dimerization of a protein, which is usually prevented in reducing conditions—although interactions can result in the appearance of higher bands

Yes. Outsourcing experiments has become a very common practice across the globe nowadays, especially in clinical investigations. The benefits of outsourcing can be substantial—from cost savings, expertise and efficiency gains to greater competitive advantage.

Yes, our team can perform 2D western blot analysis. Please submit a custom inquiry and we will reach out to you to discuss your project.