Proteomics CRO Laboratory

Western blot service

Boster offer the best and yet the most affordable Western Blot custom services. Get custom services including extraction of total proteins from tissues, construction of premade Western blots, immunostaining blots with any antibody, quantitative analysis of immunostaining, and sending high quality digital images.

Contact us for a quote

Why Choose Boster For your Western blot services ?

Because we are an antibody manufacturer and run tons of WB every week

Since 1993, Boster has been manufacturing antibodies and reagents for proteomics. With hundreds of western blot experiments performed each week and after almost 3 decades of continuous improvement, Boster's technical team has mastered the art of WB. To end of 2019 there were more than 7000 articles published using WB results obtained from Boster antibodies (source: Google scholar, Bioz and CiteAb). With such history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings, when it comes to WB service.

Western blot made easy

Source your western blot experiments to us if you:

  • 1. want to free your lab from running gels and transfers.
  • 2. are bothered by the thought of buying and testing yet one more antibody.
  • 3. don't have the time to run all those samples
  • 4. want to try out a CRO who can be your long term partner in broadening the bandwidth of your lab/team in a flexible manner.

What will I get?

Regular Western blotting: A full report with digitized WB exposure data image for each gel we run for you. Gels can have 10-15 lanes per gel.

Advanced protein characterization: We also provide free consultation to optimized the WB strategy for you if needed--WB sometimes is not sufficient in characterizing protein of interest from contaminants. We can design custom strategies such as using silver stain and LC-MS to identify contaminants.

Western Blot Service details

what to provide, turn around time, deliverable, notes

You need to provide

Purified protein (>50 ug/sample, concentration: >1 mg/ml)

Or lysate sample (total protein >500 ug/sample, concentration: >1 mg/ml)

Primary antibody to detect target protein (or purchased from us)

Turn around time

About 1 week. Actual time vary based on how many projects we have

Deliverable

Exposure data (films and membranes)

Project report

Note

Recommend to use a sample expressing the target protein as the positive control
1-8 samples per membrane