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Considering outsourcing your WB experiments? Let us help you take control over your Western blots. As a dear customer puts it, "trade money for time", with our fast turnaround Western blot service.
Send us your samples and receive data and analysis. Boster Bio is an antibody manufacturer and runs hundreds of WB every week for our internal production quality control. Our team members have 3 decades of WB know-how and a streamlined WB workflow. That's why we can do your blots at affordable prices with high quality AND fast turnaround time.
We are also a contributor of WB knowledge on the internet with thousands of visitors to our free WB resources each month.
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Increasingly more labs are outsourcing their experiments. This trend has accelerated as more labs fall behind their schedules due to the COVID-19 pandemic. Below we lay out the pros and cons of outsourcing experiments, and list a few factors to look for when outsourcing Western blotting.
While in-house WB may require significant investment (e.g. equipment cost and maintenance, labor), a specialized lab can perform western blot more cost efficiently. With WB being taken care of by a trusted lab, you can allocate your budget and resources for use on other projects or programs.
In some cases, you can outsource to other countries to save cost, such as screening/hunting for antibodies that work. Boster also provides such service--see details of our antibody hunting service here. We also receive discounts from our partnering antibody companies and can pass on the savings to you.
Working with a specialized lab enables access to expertise, more resources, and advanced technologies. The CRO lab will be capable of performing successful western blot experiments in a high-throughput manner while avoiding common WB technical issues, such as nonspecific bands, weak/no signal, saturated protein detection, high background, etc., through various optimizations (i.e. protein isolation, buffers, type of separation, gel concentration, etc.).
Success comes from focus. The 80/20 principle (Pareto's Principle) is true for Western blotting as it is for life--the key to success is freeing up your bandwidth to focus on essential objectives. By outsourcing your WB experiments, you’ll have the flexibility to devote more time, energy, and resources on other strategically important initiatives and accelerate your timelines.
Outsource WB experiments to us if you:
|Cost||++||Our cost is on the lower side|
|Turnaround||+++||Typically < 1 week|
|Service Level||+++||Personal attention is our creed|
|Special Needs||++||We try our best|
|Logistics||++||Ship samples overnight|
|Credential||+++||28+ years manufacturing antibodies|
As an antibody manufacturer, we are in a unique position to serve you as a WB CRO.
|Cost||???||In-house costs are hard to calculate|
|Service Level||--||Not applicable|
|Special Needs||+++||Your needs are not special to you|
|Logistics||+||2 days of hard work in the lab|
If you are not doing it already, it might not be a good idea to start now. WB is a laborious process and tons of scientists can do it, so adding it to your lab's arsenal will not add much to your competitive advantage. If it is not core to your lab's success, outsourcing it is worth considering.
If your lab is doing it already, but considering outsourcing it, we recommend you get a quote from us today, and see if the numbers work out.Get A Quote/Consultation
Since we do not know who we are going up against, we have no comments here. We hope you find the list of things to look out for useful in deciding who to go with.
If you ever need help sorting through the options, we are happy to help. Book an appointment today!
Tell us which commercial antibody you would like to use for WB. You can also discuss with us to determine which antibodies would work for your project.
Ship your samples with 3-5 lbs dry ice to our facility. You can also select samples for your project from Boster Bio's range of normal and disease samples.
In about 1 week, we will deliver a detailed report that includes the Western Blot results as well as the equipment, reagents, and protocol used.
Let our team of experts provide you publishable data and optimized Western Blot protocols. For any inquires or questions, contact [email protected] and a representative from Boster will directly work with you to ensure your project is conducted as per your requirements.
|Turnaround||About 1 week. Actual times may vary based on how many projects our team is handling.|
If you have questions about our service, feel free to send us a message. We will respond to you as soon as possible.
Send us a message and one of our product managers will contact you to discuss your project.
Activate the Target X pathway by stimulation and evaluate the inhibitory effects of various treatments. Assess the level of phospho-Target X by western blot.
Phosphorylation of Target X was induced by Stimulant-X stimulation. The Stimulant-X-induced phosphorylation was inhibited by Control Inhibitor but not the negative control antibody. Target X phosphorylation was also inhibited by all the testing antibodies, except for Test Inhibitor 4, at 1ug/ml. The inhibitory effect reduces as the antibody concentration decreases.
Our lab performed a western blot analysis of Histone H3 using anti-Histone H3 Antibody Picoband™ (A12477-2) in 10 different samples. A specific band was detected for Histone H3 at approximately 17KD. The expected band size for Histone H3 is at 17KD. Below is a sample WB service report.
Some western blot images generated by Boster Bio's WB team.
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|Detects the presence of antigen and antibody in cell lysates, serum, etc.||Detects the presence of a specific protein and its MW from a mixture of proteins|
|Less time-consuming||Time-consuming, delicate process, costly|
|Qualitative and quantitative||Qualitative and sometimes semi-quantitative|
|Higher specificity and less likely to give false positive results|
|Post-translational modifications||Increases the protein size (phosphorylation, glycosylation, etc.)|
|Post-translational cleavage||Most proteins are synthesized as pro-proteins and then cleaved to give the protein its active form (e.g pro-caspases)|
|Splice variants||Highly variable depending on the specific tissue and experimental conditions, which may lead to the production of different sized protein products|
|Isoforms||Many proteins express multiple isoforms that are different sizes. Expression of different isoforms for the same target protein is highly variable depending on the specific tissue and experimental conditions. Several websites can be accessed to determine isoforms for specific targets.|
|Relative charge||The composition of amino acids (charged vs. non-charged)|
|Multimers||An example is dimerization of a protein, which is usually prevented in reducing conditions—although interactions can result in the appearance of higher bands|