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Boster Bio offers Western blotting / protein characterization services with fast turnaround times. Are you looking to outsource your WB? Are you behind on your studies and need to delegate some experiments? Work with Boster Bio to boost your research and catapult your discovery trajectory.
Send us your samples and receive data and analysis. Boster Bio offers Western blotting / protein characterization services with fast turnaround times. Are you looking to outsource your WB? Are you behind on your studies and need to delegate some experiments? Work with Boster Bio to boost your research and catapult your discovery trajectory.Get Quote/ConsultationBrowse Available Antibodies
Because we are an antibody manufacturer that runs tons of WB every week
Since 1993, Boster has been manufacturing antibodies and reagents for proteomics. With hundreds of western blot experiments performed each week, almost 3 decades of experience, state-of-the-art equipment, and continuous improvement in bringing innovation to research, Boster's technical team has mastered the art of WB.
Till the end of 2019, there were more than 7,000 articles published using Western blot results obtained from Boster antibodies (sources: Google Scholar, Bioz, and CiteAb) — a reflection of the confidence that customers place in us. Through our industry-leading Western Blot service, you will have access to a unique combination of service quality, subject expertise, and cost savings.
Outsource WB experiments to us if you:
Tell us which commercial antibody you would like to use for WB. You can also discuss with us to determine which antibodies would work for your project.
Ship your samples with 3-5 lbs dry ice to our facility. You can also select samples for your project from Boster Bio's range of normal and disease samples.
In about 1 week, we will deliver a detailed report that includes the Western Blot results as well as the equipment, reagents, and protocol used.
Let our team of experts provide you publishable data and optimized Western Blot protocols. For any inquires or questions, contact [email protected] and a representative from Boster will directly work with you to ensure your project is conducted as per your requirements.
|Turnaround||About 1 week. Actual times may vary based on how many projects our team is handling.|
If you have questions about our service, feel free to send us a message. We will respond to you as soon as possible.
Send us a message and one of our product managers will contact you to discuss your project.
Activate the Target X pathway by stimulation and evaluate the inhibitory effects of various treatments. Assess the level of phospho-Target X by western blot.
Phosphorylation of Target X was induced by Stimulant-X stimulation. The Stimulant-X-induced phosphorylation was inhibited by Control Inhibitor but not the negative control antibody. Target X phosphorylation was also inhibited by all the testing antibodies, except for Test Inhibitor 4, at 1ug/ml. The inhibitory effect reduces as the antibody concentration decreases.
Our lab performed a western blot analysis of Histone H3 using anti-Histone H3 Antibody Picoband™ (A12477-2) in 10 different samples. A specific band was detected for Histone H3 at approximately 17KD. The expected band size for Histone H3 is at 17KD. Below is a sample WB service report.
Some western blot images generated by Boster Bio's WB team.
Fill out our Contact Us form and we will prepare a quote for your project.
Please contact our support team for any additional questions you have.
Despite its overall simplicity, it can be difficult to perform Western Blot successfully in a high-throughout manner to your advantage and problems during analysis happen a lot in the lab.
Western Blot is a delicate and time-consuming process that requires technical demand from scientists/technicians. Simple mistakes and/or a minute of imbalance at any level of the procedure, such as using too little or too much primary antibody, can skew the results of the entire process. However, you won’t see these failures in the literature because the unsuccessful blots won’t be published. In fact, according to a report on GEN, 41% of researchers admit that their Western blots are unsuccessful at least 25% of the time.
Some challenges of Western Blot analysis are listed below:
When compared to doing WB analysis in-house, outsourcing Western Blot offers clear advantages, which will allow you to devote more time and energy to focus on your core expertise.
|Advantages of Outsourcing Western Blot Service||Disadvantages of In-House Western Blot|
|Cost-effective to help optimize your costs and resources for use on other projects or programs||High cost, significant investment (cost of the equipment, equipment maintenance, personal cost)|
|Access to expertise, more resources, and advanced technologies of an innovative company||Lack of expertise for the specific skill set|
|Reliable results in significantly less time||May become time-consuming|
|Enables you to concentrate on your core research activities|
|Risk mitigation (schedule, budget, operational, technical, external)|
Leave us a message and request a quote. A Boster representative will get in touch with you as soon as possible.
|Detects the presence of antigen and antibody in cell lysates, serum, etc.||Detects the presence of a specific protein and its MW from a mixture of proteins|
|Less time-consuming||Time-consuming, delicate process, costly|
|Qualitative and quantitative||Qualitative and sometimes semi-quantitative|
|Higher specificity and less likely to give false positive results|
|Post-translational modifications||Increases the protein size (phosphorylation, glycosylation, etc.)|
|Post-translational cleavage||Most proteins are synthesized as pro-proteins and then cleaved to give the protein its active form (e.g pro-caspases)|
|Splice variants||Highly variable depending on the specific tissue and experimental conditions, which may lead to the production of different sized protein products|
|Isoforms||Many proteins express multiple isoforms that are different sizes. Expression of different isoforms for the same target protein is highly variable depending on the specific tissue and experimental conditions. Several websites can be accessed to determine isoforms for specific targets.|
|Relative charge||The composition of amino acids (charged vs. non-charged)|
|Multimers||An example is dimerization of a protein, which is usually prevented in reducing conditions—although interactions can result in the appearance of higher bands|