Proteomics CRO Laboratory

WESTERN BLOT SERVICE

Boster Bio offers Western blotting / protein characterization services with fast turnaround times. Are you looking to outsource your WB? Are you behind on your studies and need to delegate some experiments? Work with Boster Bio to boost your research and catapult your discovery trajectory.

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Affordable WB Service with Fast Turnaround

Send us your samples and receive data and analysis. Boster Bio offers Western blotting / protein characterization services with fast turnaround times. Are you looking to outsource your WB? Are you behind on your studies and need to delegate some experiments? Work with Boster Bio to boost your research and catapult your discovery trajectory.

Get Quote/ConsultationBrowse Available Antibodies

Why Choose Boster For Your Western blot Services?

Because we are an antibody manufacturer that runs tons of WB every week

Since 1993, Boster has been manufacturing antibodies and reagents for proteomics. With hundreds of western blot experiments performed each week, almost 3 decades of experience, state-of-the-art equipment, and continuous improvement in bringing innovation to research, Boster's technical team has mastered the art of WB.

Till the end of 2019, there were more than 7,000 articles published using Western blot results obtained from Boster antibodies (sources: Google Scholar, Bioz, and CiteAb) — a reflection of the confidence that customers place in us. Through our industry-leading Western Blot service, you will have access to a unique combination of service quality, subject expertise, and cost savings.

Western blot made easy

Outsource WB experiments to us if you:

  1. Want to free your lab from running gels and transfers.
  2. Are bothered by the thought of buying and testing yet one more antibody.
  3. Don't have time to run all samples.
  4. Need faster turnaround times while obtaining publishable data.
  5. Want a CRO as your long-term partner to broaden the bandwidth of your lab/team in a flexible manner.

What is Included in the Service?

Select Antibody

Tell us which commercial antibody you would like to use for WB. You can also discuss with us to determine which antibodies would work for your project.

Ship Your Samples

Ship your samples with 3-5 lbs dry ice to our facility. You can also select samples for your project from Boster Bio's range of normal and disease samples.

Get Results

In about 1 week, we will deliver a detailed report that includes the Western Blot results as well as the equipment, reagents, and protocol used.

How do I get started?

Let our team of experts provide you publishable data and optimized Western Blot protocols. For any inquires or questions, contact [email protected] and a representative from Boster will directly work with you to ensure your project is conducted as per your requirements.

You Provide
  • Purified protein (>50 ug/sample, concentration: >1 mg/ml) OR lysate sample (total protein >500 ug/sample, concentration: >1 mg/ml)
  • Primary antibody to detect target protein (or purchase from us)
  • Non-purified samples will require housekeeping proteins to normalize against
  • Total protein can be measured using biotin labeling agent and strep-HRP
  • It is recommended to use a sample expressing the target protein as the positive control
We Deliver
  • Free consultation for your project
  • Customized project report with publication-grade Western Blot exposure data image for each gel we run for you. Each gel can have 10-15 lanes. The report will also provide the equipment, reagents, and assay protocol that our team used for your project.
Turnaround About 1 week. Actual times may vary based on how many projects our team is handling.

Questions?

If you have questions about our service, feel free to send us a message. We will respond to you as soon as possible.

Western Blot Service Workflow

  • Sample Selection

    If you do not have samples, Boster has available normal and disease samples available.

    User-provided sample:
    Purified protein (>50 ug/sample, >1 mg/ml)
    Lysate sample (Total protein >500 ug/sample, >1 mg/ml)

    Inquire about available samples
  • Antibody Selection

    We can use any commercially available antibody for your project. If you need assistance for selecting antibodies, we can help you.

    Inquire about antibodies
  • Optimization

    Commercial antibodies are not always validated for every sample type. You may need to optimize the dilution for your project. Typical optimization is 3-5 serial dilutions to find the optimal performance.

    If you prefer, we can also work with a dilution ratio that you have already optimized.

  • Controls

    To ensure your antibody is detecting the proper protein, it is recommended to use a sample known to express the target protein as a control.

    Boster has available positive and negative controls for your assay. We can also work with your controls.

    Inquire about controls
  • Advanced Protein Characterization

    Boster Bio provides free consultation to optimize your WB strategy. Western Blot is sometimes not sufficient for characterizing protein of interest from contaminants. We can design custom strategies such as using silver stain and LC-MS to identify contaminants.

    Contact us

Get a Free Consultation

Send us a message and one of our product managers will contact you to discuss your project.

Sample Data

Project Scope

Activate the Target X pathway by stimulation and evaluate the inhibitory effects of various treatments. Assess the level of phospho-Target X by western blot.

Conclusion

Phosphorylation of Target X was induced by Stimulant-X stimulation. The Stimulant-X-induced phosphorylation was inhibited by Control Inhibitor but not the negative control antibody. Target X phosphorylation was also inhibited by all the testing antibodies, except for Test Inhibitor 4, at 1ug/ml. The inhibitory effect reduces as the antibody concentration decreases.

Sample Report

Project Scope

Our lab performed a western blot analysis of Histone H3 using anti-Histone H3 Antibody Picoband™ (A12477-2) in 10 different samples. A specific band was detected for Histone H3 at approximately 17KD. The expected band size for Histone H3 is at 17KD. Below is a sample WB service report.

More Western blotting images

Some western blot images generated by Boster Bio's WB team.

Get Started Now

Fill out our Contact Us form and we will prepare a quote for your project.
Please contact our support team for any additional questions you have.

Outsource Western Blot Analysis

Inconveniences of Doing WB Analysis By Yourself

Despite its overall simplicity, it can be difficult to perform Western Blot successfully in a high-throughout manner to your advantage and problems during analysis happen a lot in the lab.

Western Blot is a delicate and time-consuming process that requires technical demand from scientists/technicians. Simple mistakes and/or a minute of imbalance at any level of the procedure, such as using too little or too much primary antibody, can skew the results of the entire process. However, you won’t see these failures in the literature because the unsuccessful blots won’t be published. In fact, according to a report on GEN, 41% of researchers admit that their Western blots are unsuccessful at least 25% of the time.


Some challenges of Western Blot analysis are listed below:

  • Highly specific primary antibodies against the protein of interest is crucial to detect that particular protein when performing WB. Although many antibodies are commercially available, they sometimes do not detect the target protein when tested in the laboratory with particular tissues or cell types.
  • WB can be time-consuming and requires various optimizations (i.e. protein isolation, buffers, type of separation, gel concentration, etc.).
  • The antibodies may not recognize the immobilized antigen in its denatured state. Sometimes, the antibodies interact with other proteins and label an incorrect protein (off-target effects).
  • Transfer inefficiency when small proteins are not retained by the membrane and larger proteins are not transferred properly due to varying gel concentrations and transfer time, causing erroneous bands or no bands at all.
  • Common WB issues that occur, such as nonspecific bands, weak/no signal, saturated protein detection, high background, etc.

Why Outsource Western Blot?

When compared to doing WB analysis in-house, outsourcing Western Blot offers clear advantages, which will allow you to devote more time and energy to focus on your core expertise.

Advantages of Outsourcing Western Blot Service Disadvantages of In-House Western Blot
Cost-effective to help optimize your costs and resources for use on other projects or programs High cost, significant investment (cost of the equipment, equipment maintenance, personal cost)
Access to expertise, more resources, and advanced technologies of an innovative company Lack of expertise for the specific skill set
Reliable results in significantly less time May become time-consuming
Enables you to concentrate on your core research activities
Risk mitigation (schedule, budget, operational, technical, external)

Advantages of Choosing Boster For Your Western Blot

  • Data analysis services at a competitive rate that will help you cut down expenses while getting accurate and reliable data
  • Flexibility by freeing up your time and resources so you can get tough projects started, focus on other strategically important initiatives, and accelerate your timelines
  • High sensitivity and specificity, able to target low analyte levels in small sample volume
  • Robust matrix tolerance including serum, plasma, and tissue culture media (TCM) as well as various complex biological matrices
  • Standard, customized, and comprehensive quality and consistency from our dedicated team of experts at every stage of your project

Ready to talk to us about your project?

Leave us a message and request a quote. A Boster representative will get in touch with you as soon as possible.

FAQs

Protein expression in cells has been an important cornerstone in biomedical research and industrial settings for decades. It plays a significant role in the production of therapeutic protein, monoclonal antibodies, and in the studies of cell signaling, gene regulation, protein-protein interactions, etc.

Representing at least 8-9% of all protein-related publications within the last decade, Western Blotting is estimated to be the most used technique across a broad range of scientific and clinical disciplines. Their ability to confirm the identity/presence of a specific protein both by size and through the binding of an antibody provides additional information not readily gathered from other key immunological laboratory techniques. Their results are less ambiguous, making them suitable as a key supplemental screen to be used in combination or provide confirmation of results from other techniques (ELISA, IHC, etc.)

Western blotting is informative for various instances, such as:
  • Evaluating levels of protein expression in cells
  • Monitoring fractions during protein purification
  • Comparing expression of a target protein from various tissues
  • Determining how a particular protein responds to disease or drug treatment
  • Determining the effect of time on a protein

The data produced with a Western Blot is typically considered to be qualitative and sometimes semi-quantitative, as it provides a relative comparison of protein levels, but not an absolute measure of quantity.

Both Western Blot and ELISA are two very different methods based on immunodetection. The main differences are as follows:
ELISA Western Blot
Detects the presence of antigen and antibody in cell lysates, serum, etc. Detects the presence of a specific protein and its MW from a mixture of proteins
Less time-consuming Time-consuming, delicate process, costly
Qualitative and quantitative Qualitative and sometimes semi-quantitative

Higher specificity and less likely to give false positive results

Western blotting is a technique that separates proteins by size. The smaller the protein is, the faster it migrates through the gel. However, the migration speed is also affected by other factors, so the actual band size observed may differ from what was predicted.
Possible Reasons Notes
Post-translational modifications Increases the protein size (phosphorylation, glycosylation, etc.)
Post-translational cleavage Most proteins are synthesized as pro-proteins and then cleaved to give the protein its active form (e.g pro-caspases)
Splice variants Highly variable depending on the specific tissue and experimental conditions, which may lead to the production of different sized protein products
Isoforms Many proteins express multiple isoforms that are different sizes. Expression of different isoforms for the same target protein is highly variable depending on the specific tissue and experimental conditions. Several websites can be accessed to determine isoforms for specific targets.
Relative charge The composition of amino acids (charged vs. non-charged)
Multimers An example is dimerization of a protein, which is usually prevented in reducing conditions—although interactions can result in the appearance of higher bands

Yes. Outsourcing experiments has become a very common practice across the globe nowadays, especially in clinical investigations. The benefits of outsourcing can be substantial—from cost savings, expertise and efficiency gains to greater competitive advantage.

Yes, our team can perform 2D western blot analysis. Please submit a custom inquiry and we will reach out to you to discuss your project.