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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
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Protocols, optimization tips,troubleshooting guides, and more for flow cytometry.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.
Note: This information is to serve as a guide as individual investigators may need to optimize protocols for their particular cell type.
Cells for flow cytometry analysis are usually derived from four main sources:
Regardless of the source, the final cell preparation should be:
PBMCs isolated from whole blood through Ficoll gradient centrifugation or RBC lysed whole blood or non-adherent cultured cells are readily available for flow cytometric analysis. Adherent cultured cells or cells present in the solid organs should be first made into a single cell suspension before flow analysis by using enzymatic digestion or mechanical dissociation of the tissue, respectively. Mechanical filtration should be followed to avoid unwanted instrument clogs or lower quality flow data. Use the following sample preparation protocols based on your appropriate starting materials:
Key Reagents – PBS, staining buffer
Key Reagents – PBS, staining buffer, 0.25% trypsin
Key reagents – PBS, staining buffer, culture medium with 10% FBS
Key reagents – PBS, staining buffer, suitable gradient medium like Ficoll or Histopaque
Key reagents – PBS, staining buffer (see appendix), RBC lysis buffer [check Appendix for recipe or use a commercially available buffer]