Rabbit Monoclonal Antibody Advantages And Production

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Need to create custom Rabbit monoclonal antibodies ?

Explore Boster Bio's state of art RABBIT MONOCLONAL ANTIBODY DISCOVERY SERVICE. Our plasma cell discovery (PCD) platform is the best solution for clinical and diagnostic antibody discovery.

What Are Rabbit Monoclonal Antibodies?

Monoclonal antibodies are used as primary antibodies in research because of their high specificity for a unique epitope of an antigen, which also leads to minimal lot-to-lot variation and low non-specific cross-reactivity. After the discovery of the hybridoma technique designed by Caesar Millstein and Georges Kohler to produce monoclonal antibodies in 1975, monoclonal antibodies have been conventionally developed in mice for various applications. Over the years, other new techniques have led to the emergence of monoclonal antibodies raised against rabbits, rats, and hamsters. Although mouse monoclonal antibodies have remained as the most commonly used type of monoclonal antibody, a 2015 CiteAb study observed accelerating growth in the number of researchers using rabbit monoclonal antibodies while mouse monoclonals have experienced a steady decrease in the percentage of citations.

Why is rabbit monoclonal antibodies better ?

The rabbit immune system can produce greater antibody diversity due to the factors below.

Somatic Gene Conversion & Somatic Hypermutation

Somatic Gene Conversion & Somatic Hypermutation

Somatic gene conversion and hypermutation influence the variable regions of immunoglobulin (Ig) genes, which happen more often in rabbits than rodents. This ultimately contributes to enhancing the rabbit monoclonal antibody repertoire.

IgG Vs IgM

IgG Vs IgM

Rabbits have more IgG as an isotype compared to mice which have a higher ratio of IgM. Because IgG has better specificity than IgM, rabbits have more potential to develop specific antibodies.

CDRs (Complementarity-Determining Regions)

CDRs (Complementarity-Determining Regions)

Since rabbit CDR3 sequences are longer and more heterogeneous, more sequence variation can occur.

Lower Immunodominance

Lower Immunodominance

Immunodominance refers to particular epitopes being more immunogenic than other epitopes of the same antigen, which causes the immune system to mainly generate antibodies against the dominantly immunogenic epitope. The rabbit’s immune system has lower immunodominance than mouse, which allows for more antibody diversity.

Larger Number Of Lymphocytes

Larger Number Of Lymphocytes

Rabbits have larger spleens and other immune organs than mice, which indicates that more lymphocytes (B cells) are available for producing antibodies.

Extended Period Of Affinity Maturation

Extended Period Of Affinity Maturation

After immunization, affinity maturation can persist for a longer period of time for rabbits than mice, resulting in higher affinity and specificity for rabbit monoclonal antibodies.

Benefits Of Rabbit Monoclonal Antibodies

Due to the unique features of the rabbit immune system, rabbit monoclonal antibodies are capable of offering the following benefits:

High Specificity & Affinity

Rabbit monoclonal antibodies are effective in recognizing minor epitope variations and cleaved versions of a target protein. Furthermore, rabbit monoclonal antibodies can have extremely low equilibrium dissociation constants (KD) in the picomolar range (KD = 10-12M).

Low Background Noise

As rabbit monoclonal antibodies only detect a single epitope, they have a low chance of cross-reacting with other proteins. Since rabbit monoclonal antibodies also bind to the target antigen with greater affinity, they prove to have a higher signal-to-noise ratio than mouse monoclonal antibodies.

Ideal For IHC

For IHC, antibodies need to be able to identify epitopes in a background with more noise (i.e. FFPE tissues). In this case, rabbit monoclonal antibodies are an excellent choice because they can be highly sensitive while retaining specificity even in strict applications like IHC.

Good For Post-Translational Modification (PTM) Detection

The rabbit immune system can produce antibodies against small epitopes existing in small molecules, lipids, and polymers, which permit rabbit monoclonal antibodies to identify subtle changes from PTMs (e.g., phosphorylation, methylation, acetylation, sumoylation, etc.) or single amino acid substitutions.

Novel Epitope Recognition For Human, Mouse, And Rat Targets

In comparison to the mouse immune system, the rabbit immune system possesses reduced immunodominance and greater B-cell diversity, which enables wider epitope detection and a higher possibility of discovering unique epitopes when developing rabbit monoclonal antibodies.

Validated For Flow, IHC, WB, ICC, IF, IP

Our rabbit monoclonal antibodies have been validated for various applications including WB, IHC, ICC/IF, IP, and flow cytometry as well as common species like human, mouse, and rat.

Rabbit Monoclonal Vs. Mouse Monoclonal

Comparison Rabbit Monoclonal Antibodies Mouse Monoclonal Antibodies
Antigen Detection
  • Better at identifying subtle differences (e.g., epitope variations, like modifications, mutations, conformational changes)
  • Able to detect a wider range of antigens, like small molecules and peptides
  • Identifies multiple epitopes per target antigen
  • Often non-immunogenic for small molecules and peptides
  • Immunodominance limits the number of epitopes recognized
  • 10-100x higher affinity for target antigen than mouse monoclonal antibodies (Picomolar range: 10-12 KD M)
  • Enhanced affinity, including antigens that are poorly immunogenic in mice and humans
  • Lower affinity than rabbit monoclonal antibodies (Nanomolar range: ~10-9 KD M)
Specificity High Medium or high

Rabbit Monoclonal Antibody Production

The general stages of rabbit monoclonal antibody production consist of rabbit immunization, plasma cell isolation, cloning and expression, and screening and selection. The rabbit monoclonal antibodies produced from a single clone are characterized for specific applications (e.g., ELISA, WB, IHC, etc.) and the best clone is chosen for scaling up production and performing purification. Because of the expensive and time-consuming process (usually 4-6 months) of monoclonal antibody production, they are typically more expensive than polyclonal antibodies.

Immunize rabbits

Isolate rabbit spleen cells

Fuse with myeloma partner cells

Recover hybridoma cells

Screen for monospecific clones

Produce best antibodies on small scales

Characterize antibodies by application

Scale up production and purification

Custom Rabbit Monoclonal Antibodies

Bosterbio provides custom antibody production and development services for rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies. For more details about how our service can help your research, visit our custom rabbit monoclonal antibody production service page to see service information, deliverables, and expected costs. If you have any questions about your custom rabbit monoclonal antibody project, please feel free to contact us to discuss and request a quote.

Need to create custom Rabbit monoclonal antibodies ?

Explore Boster Bio's state of art RABBIT MONOCLONAL ANTIBODY DISCOVERY SERVICE. Our plasma cell discovery (PCD) platform is the best solution for clinical and diagnostic antibody discovery.

Boster Rabbit Monoclonal Antibodies In Research

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Protocol Overview

Immunohistochemistry (IHC-P)

  • Tissue Preparation
  • Deparaffinization
  • Inactivation
  • Antigen Retrieval
  • Blocking
  • Primary Antibody Incubation
  • Secondary Antibody Incubation
  • Staining
View IHC-P & IHC-F Protocols

Western Blot

  • Protein Extraction
  • Protein Quantitation Assay
  • Gel Electrophoresis
  • Protein Transfer
  • Blocking
  • Primary Antibody Incubation
  • Secondary Antibody Incubation
  • Chromogenic or Chemiluminescent Detection
View WB Protocol

Flow Cytometry

  • Direct immunostaining of surface antigens
  • Indirect immunostaining of surface antigens
  • General immuno-staining procedure for intracellular antigens
  • Intracellular cytokine/phospho-immunostaining
  • In Vitro Cell Stimulation Reference Table
  • Dye efflux staining
  • DNA content or Cell cycle analysis
View Flow Cytometry Protocol

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