PicoKine® Quick ELISA kit

Principle of PicoKine® Quick ELISA kits:

The Boster Picokine® Fast ELISA is a solid phase immunoassay, in which micro-titration wells are pre-coated with a capture antibody specific to the target analyte. Unlike traditional ELISA, sample and biotinylated detection antibody are incubated collectively, allowing the formation of sandwich complex in a single step. Avidin-Biotin-Peroxidase Complex (ABC-HRP) is added in the next step, which will act as an enzyme for TMB substrate, producing a blue colored product. The intensity of the colored product is directly proportional to the amount of target analyte.

Sensitivity comparison

Sensitivity is the lowest detectable amount of a target analyte and is one of the most important factors in choosing your ELISA kit. Our Picokine® Quick ELISA kits ensure high turnaround time without any compromise on the quality of your results.

To analyze our sensitivity, we compared Picokine® Quick ELISA with competitors 90 minute ELISA and concluded that Picokine® Quick ELISA kits offer better sensitivity, as shown in the graph below:

Guarantee of reproducibility and lot to lot consistency:

Reproducibility is one of the most important factor in choosing your ELISA kit as it provides information about the precision of the kit and its components. We ensure high reproducibility of our PicoKine® Quick ELISA kits by evaluating the performance consistency using different concentrations of target protein in different lots. Our results show Coefficient of Variation (CV) less than 10% in all cases which demonstrate high precision and accuracy.

Stability:

Bosters PicoKine® Quick ELISA kits are tested for stability. In the graph below, three samples with different concentratiots of a target protein are assayed using the same kit over a period of 12 months.

Specificity and cross reactivity:

Boster selectively evaluates the target analyte for the presence of other proteins which may lead to false positive results and ensures no cross-reactivity in our kits. We look at homology with endogenous proteins, if there is any cross reactivity we will list it on the product page.

Recovery:

The spiking recovery test, a method to evaluate the accuracy, was analyzed by adding a known amount of analyte to the sample matrices, such as serum and plasma. Recovery was calculated by comparing the observed concentration as determined by ELISA and the expected concentration by summation. Low, medium, and high concentrations of analyte are spiked into all validated sample types and then analyzed for recovery. The results are expressed as a percentage of analyte recovered. Our acceptance range for the recovery is 80–120%. Low, medium, and high concentrations of recombinant human BDNF were used as the spike in the spike and recovery experiments.