PicoKine® Quick ELISA kit

Your upgrade to traditional ELISA; save up to two hours of your valuable time without compromising on the results.

Featured Quick ELISA Kits:

Browse All Quick PicoKine® ELISA Kits


Picokine® Quick ELISA

Since early 2000, Boster has developed its proprietary ELISA platform that allows supreme sensitivity in detection as well as shortening the duration of ELISA protocol by combining the sample incubation and the detection antibody incubation step. These Quick ELISA kits, also known as one-step ELISA kits, require only 1.5 hours to run and have comparable quality to traditional sandwich ELISA kits. Browse all the one-step Quick ELISA kits here.


Why choose Boster's Picokine® Quick ELISA Kit?

If you’re looking for a rapid yet reliable ELISA kit, which ensures high quality results, with significant quick turnaround time, look no further, because Boster Bio got you covered. Picokine® Quick ELISA kits are the upgrade to your traditional, lengthy ELISA protocols, delivering excellent results in less than 90 minutes.

PicoKine® Quick ELISA kit features:

  • The rapid version of Picokine ELISA kits, PicoKine® Quick ELISA, offers high sensitivity with quick turnaround time.

  • Reduced assay duration and simplified protocol, as compared to traditional ELISA procedures. The assay takes less than 1.5 hours, with no compromise on your results.

  • Validated on wide range of biological samples.

  • Guarantee in consistency and reproducibility in the results.

  • The kit size is 96 wells/kit with removable strips and ready to use reagents.


What's the big deal?

What PicoKine® Quick ELISA kit offers?How it benefits you?
90 minutes quick assay as compared to 2.5 hours long traditional ELISAFaster turnaround means generation of more results and publications in a shorter duration of time.
High sensitivity, consistency and reproducibility of results.Obtain accurate and reliable data you can trust.
Simplified protocol as compared to traditional ELISA.Simple and easy to perform protocol mean less chances of contamination and error.
Tested and validated on wide range of biological samples.Guaranteed reaction with the target analyte in variety of biological samples.

quick-elisa-comics



How does PicoKine® Quick ELISA differs from traditional elisa?

Traditional ELISA PicoKine® Quick ELISADifference
Assay Duration3.5 hours1.5 hoursQuick turnaround time
Sample Quantity100 uL50 uLConvenient processing of samples having limited volumes
Principle of AssaySample and biotinylated detection antibody are incubated in separate stepsSample and biotinylated detection antibody are incubated in a single stepTime effective and user friendly procedure



Principle of PicoKine® Quick ELISA kits:


The Boster Picokine® Fast ELISA is a solid phase immunoassay, in which micro-titration wells are pre-coated with a capture antibody specific to the target analyte. Unlike traditional ELISA, sample and biotinylated detection antibody are incubated collectively, allowing the formation of sandwich complex in a single step. Avidin-Biotin-Peroxidase Complex (ABC-HRP) is added in the next step, which will act as an enzyme for TMB substrate, producing a blue colored product. The intensity of the colored product is directly proportional to the amount of target analyte.


Samples validated for PicoKine® Quick ELISA kits

PicoKine® Quick ELISA kit is analytically validated on a wide range of biological samples and offers a variety of target analytes for your research, from cell culture supernatants to serum and plasma.

PicoKine® Quick ELISA kits components:

Pre-coated 96-well Strip Microplate.

Standard

Detection Antibody

Sample Diluent

Wash Buffer

Detection Antibody Diluent

Color Developing Reagent (TMB)

Stop Solution

Plate Sealers




Materials that are required but not provided:

Microplate Reader capable of reading absorbance at 450nm.

Automated plate washer (optional)

Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.

Multichannel pipettes are recommended for large amount of samples.

Deionized or distilled water.

500ml graduated cylinders.

Test tubes for dilution.





Sensitivity comparison

Bosters Picokine® Quick ELISA vs Competitors 90 minute ELISA


Sensitivity is the lowest detectable amount of a target analyte and is one of the most important factors in choosing your ELISA kit. Our Picokine® Quick ELISA kits ensure high turnaround time without any compromise on the quality of your results.

To analyze our sensitivity, we compared Picokine® Quick ELISA with competitors 90 minute ELISA and concluded that Picokine® Quick ELISA kits offer better sensitivity, as shown in the graph below:

Guarantee of reproducibility and lot to lot consistency:


Reproducibility is one of the most important factor in choosing your ELISA kit as it provides information about the precision of the kit and its components. We ensure high reproducibility of our PicoKine® Quick ELISA kits by evaluating the performance consistency using different concentrations of target protein in different lots. Our results show Coefficient of Variation (CV) less than 10% in all cases which demonstrate high precision and accuracy.


Stability:


Bosters PicoKine® Quick ELISA kits are tested for stability. In the graph below, three samples with different concentratiots of a target protein are assayed using the same kit over a period of 12 months.


Specificity and cross reactivity:


Boster selectively evaluates the target analyte for the presence of other proteins which may lead to false positive results and ensures no cross-reactivity in our kits. We look at homology with endogenous proteins, if there is any cross reactivity we will list it on the product page.

Recovery:


The spiking recovery test, a method to evaluate the accuracy, was analyzed by adding a known amount of analyte to the sample matrices, such as serum and plasma. Recovery was calculated by comparing the observed concentration as determined by ELISA and the expected concentration by summation. Low, medium, and high concentrations of analyte are spiked into all validated sample types and then analyzed for recovery. The results are expressed as a percentage of analyte recovered. Our acceptance range for the recovery is 80–120%. Low, medium, and high concentrations of recombinant human BDNF were used as the spike in the spike and recovery experiments.

Related Pages

ELISA Principle

Learn the concept behind ELISA assay. Get to know how to maximize the sensitivity and precision of the assay, the plate must be carefully coated with high-affinity antibodies – a process that Boster Bio has mastered.


Learn ELISA Principle

ELISA Sample Preparation

ELISAs can accurately assess soluble proteins in their native state, so they are ideal for samples such as urine or saliva. Check out the ELISA sample preparation guides to learn how to get the best results from your sample type.

See ELISA Sample Preparation

ELISA Troubleshooting Tips

Get To Know Some ELISA Troubleshooting Tips With This Guide. It Has Some Commonly Encountered Problems And Solutions To ELISA.



Check our ELISA troubleshooting tips

ELISA Data Analysis

Learn how to perform ELISA data analysis. Get to know the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create a standard curve for analysis.


Browse ELISA data analysis