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Your upgrade to traditional ELISA; save up to two hours of your valuable time without compromising on the results.
Featured Quick ELISA Kits:
Since early 2000, Boster has developed its proprietary ELISA platform that allows supreme sensitivity in detection as well as shortening the duration of ELISA protocol by combining the sample incubation and the detection antibody incubation step. These Quick ELISA kits, also known as one-step ELISA kits, require only 1.5 hours to run and have comparable quality to traditional sandwich ELISA kits. Browse all the one-step Quick ELISA kits here.
If you’re looking for a rapid yet reliable ELISA kit, which ensures high quality results, with significant quick turnaround time, look no further, because Boster Bio got you covered. Picokine® Quick ELISA kits are the upgrade to your traditional, lengthy ELISA protocols, delivering excellent results in less than 90 minutes.
|What does the PicoKine® Quick ELISA kit offer?||How does it benefit you?|
|90 minutes quick assay as compared to 2.5 hours long traditional ELISA||Faster turnaround means generation of more results and publications in a shorter duration of time.|
|High sensitivity, consistency and reproducibility of results.||Obtain accurate and reliable data you can trust.|
|Simplified protocol as compared to traditional ELISA.||Simple and easy to perform protocol mean less chances of contamination and error.|
|Tested and validated on wide range of biological samples.||Guaranteed reaction with the target analyte in variety of biological samples.|
|Traditional ELISA||PicoKine® Quick ELISA||Difference|
|Assay Duration||3.5 hours||1.5 hours||Quick turnaround time|
|Sample Quantity||100 uL||50 uL||Convenient processing of samples having limited volumes|
|Principle of Assay||Sample and biotinylated detection antibody are incubated in separate steps||Sample and biotinylated detection antibody are incubated in a single step||Time effective and user friendly procedure|
The Boster Picokine® Fast ELISA is a solid phase immunoassay, in which micro-titration wells are pre-coated with a capture antibody specific to the target analyte. Unlike traditional ELISA, sample and biotinylated detection antibody are incubated collectively, allowing the formation of sandwich complex in a single step. Avidin-Biotin-Peroxidase Complex (ABC-HRP) is added in the next step, which will act as an enzyme for TMB substrate, producing a blue colored product. The intensity of the colored product is directly proportional to the amount of target analyte.
PicoKine® Quick ELISA kit is analytically validated on a wide range of biological samples and offers a variety of target analytes for your research, from cell culture supernatants to serum and plasma.
• Pre-coated 96-well Strip Microplate.
• Detection Antibody
• Sample Diluent
• Wash Buffer
• Detection Antibody Diluent
• Color Developing Reagent (TMB)
• Stop Solution
• Plate Sealers
• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
Sensitivity is the lowest detectable amount of a target analyte and is one of the most important factors in choosing your ELISA kit. Our Picokine® Quick ELISA kits ensure high turnaround time without any compromise on the quality of your results.
To analyze our sensitivity, we compared Picokine® Quick ELISA with competitors 90 minute ELISA and concluded that Picokine® Quick ELISA kits offer better sensitivity, as shown in the graph below:
Reproducibility is one of the most important factor in choosing your ELISA kit as it provides information about the precision of the kit and its components. We ensure high reproducibility of our PicoKine® Quick ELISA kits by evaluating the performance consistency using different concentrations of target protein in different lots. Our results show Coefficient of Variation (CV) less than 10% in all cases which demonstrate high precision and accuracy.
Bosters PicoKine® Quick ELISA kits are tested for stability. In the graph below, three samples with different concentratiots of a target protein are assayed using the same kit over a period of 12 months.
Boster selectively evaluates the target analyte for the presence of other proteins which may lead to false positive results and ensures no cross-reactivity in our kits. We look at homology with endogenous proteins, if there is any cross reactivity we will list it on the product page.
The spiking recovery test, a method to evaluate the accuracy, was analyzed by adding a known amount of analyte to the sample matrices, such as serum and plasma. Recovery was calculated by comparing the observed concentration as determined by ELISA and the expected concentration by summation. Low, medium, and high concentrations of analyte are spiked into all validated sample types and then analyzed for recovery. The results are expressed as a percentage of analyte recovered. Our acceptance range for the recovery is 80–120%. Low, medium, and high concentrations of recombinant human BDNF were used as the spike in the spike and recovery experiments.
Learn the concept behind ELISA assay. Get to know how to maximize the sensitivity and precision of the assay, the plate must be carefully coated with high-affinity antibodies – a process that Boster Bio has mastered.
Learn ELISA Principle
ELISAs can accurately assess soluble proteins in their native state, so they are ideal for samples such as urine or saliva. Check out the ELISA sample preparation guides to learn how to get the best results from your sample type.See ELISA Sample Preparation
Check our ELISA troubleshooting tips
Learn how to perform ELISA data analysis. Get to know the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create a standard curve for analysis.
Browse ELISA data analysis