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Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others.
Keywords: IHC, optimization, fixative, pfa, perfusion, immersion, fixation methodLearn More About IHC Fixatives
Once your tissue sample is fixed using the appropriate method and fixative, it is ready for sectioning and mounting. In order to preserve tissue morphology during sectioning, the tissue must be embedded in paraffin.
Keywords: IHC, optimization, ICH-P, IHC-F, FFPE, frozen section, paraffin embedding, paraffin embedded vs frozenLearn More About IHC Embedding
There are two common methods of epitope retrieval: heat-induced epitope retrieval (HIER), and proteolytic-induced epitope retrieval (PIER). Selecting the right method is critical to get the best results for your application.
Keywords: IHC, optimization, HIER vs PIER, heat-induced epitope retrieval, proteolytic-enduce epitope retrieval, antigen retrievalLearn More About IHC Antigen Retrieval
The principle behind Immunohistochemistry (IHC) entails detection of antigen or happens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Learn more about this in this guide.Learn the concept of IHC Principle
BosterBio has a detailed stepwise IHC protocol with a clearly illustrated IHC workflow with recommended reagents. Learn how to effectively implement a successful immunostaining for tissue sections and cell climbing slices.Learn our IHC Protocol
This guide provides a thorough list of Immunohistochemistry troubleshooting tips, including weak staining, high background, nonspecific staining among others. Learn how to take control of your IHC process.Check our IHC troubleshooting tips
Learn the best IHC and ICC sample preparation techniques. Get a detailed procedure of preparing different types of preserved tissues which is key to getting high quality staining during Immunohistochemistry (IHC)..Check out our IHC Sample Preparation