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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
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Protocols, optimization tips,troubleshooting guides, and more for IHC.
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Fixation prevents autolysis and necrosis of excised tissues, preserving their morphology and antigenicity as well as increasing their resistance to processing. The choice of fixative is critical as different fixatives are more optimal for some antigens than others.
Keywords: IHC, optimization, fixative, pfa, perfusion, immersion, fixation method
Once your tissue sample is fixed using the appropriate method and fixative, it is ready for sectioning and mounting. In order to preserve tissue morphology during sectioning, the tissue must be embedded in paraffin.
Keywords: IHC, optimization, ICH-P, IHC-F, FFPE, frozen section, paraffin embedding, paraffin embedded vs frozen
There are two common methods of epitope retrieval: heat-induced epitope retrieval (HIER), and proteolytic-induced epitope retrieval (PIER). Selecting the right method is critical to get the best results for your application.
Keywords: IHC, optimization, HIER vs PIER, heat-induced epitope retrieval, proteolytic-enduce epitope retrieval, antigen retrieval