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You can pick out the top 0.001% clones from the entire splenocyte population with the help of our microfluidics plasma cell discovery (PCD) platform. The scale of phage display meets the power of in vivo affinity maturation--antibody discovery has never been so robust.
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Boster Bio's plasma-cell discovery (PCD) technology uses flow cytometry to screen individual cells treated with proprietary chemistry. This unique compound keeps B cells from secreting antibodies, retains antibodies on cell membranes. Subsequently the splenocytes are incubated with fluorochrome conjugated antigens and the plasma cells with the highest brightness are screened out for down stream screening and processing. We also have trade-secrete techniques to enhance the immunization efficiency.
Get a free consultationIn theory we can screen the entire spleen of a rabbit. This makes a huge difference. A New Zealand White Rabbit's spleen is 50mm long on average, roughly 100 times larger than a mouse spleen, containing tens if not hundreds of billions of cells. Traditional rabbit hybridoma screening can investigate hundreds of successfully fused hybridomas. Our technology screens typically 10% of the whole spleen. The difference in scale is that of a village community survey vs. a world wide census. We can find the clones with the highest affinity for you. This is especially important if you have a difficult antigen to make antibodies against.
"Novel clinical biomarkers are increasingly more difficult to make. The easy ones have already been discovered and commercialized. Thus in antibody discovery, finding that top 0.1%, even 0.01% highest affinity B-cell population is key to breakthrough."
3 core concepts, move your mouse over to see details.
When building an antibody for diagnostic assays or therapeutic candidate, recombinant antibody is the go to choice. It has the following benefits:
Rabbit monoclonal antibodies have been publicized as a better alternative to mouse monoclonal antibodies. This advantage is attributable to the fact that rabbit has a bigger repertoire for hypervariable regions that codes the CDRs in the antigen-binding site. The more options to choose from, the higher the likelihood to come up with "the perfect solution"--a high affinity antibody with a bright clinical future.
The key to discovering highest affinity clones is to screen as many clones as possible. Boster Bio's microfluidics plasma cell discovery (PCD) platform can do just that. Because no fusion step is required, the cells do not need to stay alive. The cells are fixed our PCD platform can screen for as long as you need to. We can cover the entire spleen and have done so in some cases. Typically 7 days of screening is sufficient for generating very high affinity clones.
Custom Rabbit Monoclonal Antibody Production Overview And Timeline.
Boster's custom rabbit monoclonal antibody development platform uses Single Plasma Cell Interrogation Technology, which screens the immune repertoire of rabbits. Our aim is to produce an antibody that works for your specific application. Our technical staff will guide you through the project, from designing the antigen, immunization, screening strategy, antibody validation, to the proper handling of the plasmid that expresses your antibody. We can also generate antigens for you. Our antigen synthesis service cover both peptides and recombinant proteins. We are capable to express the antigen in E. coli, baculovirus, and mammalian cell lines.
The final deliverable is the sequences of selected positive clones, and plasmids containing the cloned antibody genes. Recombinant antibodies ensure the preservation of genetic information.
Phase 1 | Duration | Deliverable |
---|---|---|
Peptide and Immunization: (2 rabbits) | Titer Data | |
Peptide synthesis 10mg, >90% purity | 1 week | Titer Data |
Peptide conjugation KLH for immunization and BSA-biotin conjugation for probing and screening | 1 week | Titer Data |
Immunization for 2 rabbits | 2-3 months | Titer Data |
Titer ELISA Assay Monitoring anti-sera titer and selecting the best animal for monoclonal |
Phase 2 | Duration | Deliverable |
---|---|---|
First Screening with FACS (> a million B cells)
|
2 weeks | |
Supernatant production 0.25 ml scale, and Secondary screening with ELISA | 1 week | ELISA data |
Phase 3 | Duration | Deliverable |
---|---|---|
Customer further screening with supernatant if necessary, choose three best clones. | ||
Clone Deconvolution, cloning, expression/ELISA conformation; 30ml scale production, yield~ 0.5mg Ab per clone | 3-4 week | Sequences |
Total | 4-5 months |
What you will receive upon successful completion of above phases.