Discover the top 0.001% high affinity clones

rabbit monoclonal antibody discovery service (recombinant)

You can pick out the top 0.001% clones from the entire splenocyte population with the help of our microfluidics plasma cell discovery (PCD) platform. The scale of phage display meets the power of in vivo affinity maturation--antibody discovery has never been so robust.

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Technology overview

Technology Principle

Screen As Early As Possible, Screen As Much As Possible

Boster Bio's plasma-cell discovery (PCD) technology uses flow cytometry to screen individual cells treated with proprietary chemistry. This unique compound keeps B cells from secreting antibodies, retains antibodies on cell membranes. Subsequently the splenocytes are incubated with fluorochrome conjugated antigens and the plasma cells with the highest brightness are screened out for down stream screening and processing. We also have trade-secrete techniques to enhance the immunization efficiency.

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Put it in context of scale

In theory we can screen the entire spleen of a rabbit. This makes a huge difference. A New Zealand White Rabbit's spleen is 50mm long on average, roughly 100 times larger than a mouse spleen, containing tens if not hundreds of billions of cells. Traditional rabbit hybridoma screening can investigate hundreds of successfully fused hybridomas. Our technology screens typically 10% of the whole spleen. The difference in scale is that of a village community survey vs. a world wide census. We can find the clones with the highest affinity for you. This is especially important if you have a difficult antigen to make antibodies against.

"Novel clinical biomarkers are increasingly more difficult to make. The easy ones have already been discovered and commercialized. Thus in antibody discovery, finding that top 0.1%, even 0.01% highest affinity B-cell population is key to breakthrough."

Key concepts/benefits

3 core concepts, move your mouse over to see details.

Recombinant antibody is good

Optimal for Diagnostics and Therapeutics

When building an antibody for diagnostic assays or therapeutic candidate, recombinant antibody is the go to choice. It has the following benefits:

  1. Sequence is available for downstream development such as humanization.
  2. It is stable and scalable.
  3. Its sensitivity and specificity can be further improved by antibody engineering.

Rabbit is better than mouse

Rabbit has a big CDR pool

Rabbit monoclonal antibodies have been publicized as a better alternative to mouse monoclonal antibodies. This advantage is attributable to the fact that rabbit has a bigger repertoire for hypervariable regions that codes the CDRs in the antigen-binding site. The more options to choose from, the higher the likelihood to come up with "the perfect solution"--a high affinity antibody with a bright clinical future.

Finding rare population is key

Screen as much and as early as possible

The key to discovering highest affinity clones is to screen as many clones as possible. Boster Bio's microfluidics plasma cell discovery (PCD) platform can do just that. Because no fusion step is required, the cells do not need to stay alive. The cells are fixed our PCD platform can screen for as long as you need to. We can cover the entire spleen and have done so in some cases. Typically 7 days of screening is sufficient for generating very high affinity clones.

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Our technology vs. alternatives

Boster Bio Recombinant Rabbit monoclonal

Affinity+++High
CDR Pool+++Large
Cost+++High
Scalability+++High
Turnaround+Slow
UseDiagnotics & Theraputics

Best choice for diagnostic and Therapeutic antibodies

Recombinant rabbit monoclonal antibody production features high affinity, easy humanization and high scalability. The pricing is more than what one would expect for developing research only but it is very affordable for diagnostic and clinical antibody discovery projects. The turnaround time is around 6 months.

vs.

Hybridoma rabbit monoclonal

Affinity++Hit&miss
CDR Pool+++Large
Cost+++High
Scalability+++High
Turnaround+Slow
UseDiagnotics & Theraputics

Last generation RabMab technology

Even though it shares the same same CDR repertoire, hybridoma rabbit monoclonal technology falls short on the screening scale. The last generation rabbit monoclonal technique only screen thousands of average splenocytes that were lucky enough to successfully fuse while as we can screen the whole spleen if we want to. Our workflow does not require to keep the cells alive while screening and we go for the cream of the crop only -- plasma cells, with the brightest signals.

Mouse Monoclonal

Affinity+Low
CDR Pool+small
Cost++Mid
Scalability+++High
Turnaround+Slow
UseDiagnotics & Research

A mature technology, had its days, still good for research

Mice have limited CDR repertoires. In general mammals with longer reproductive cycles tend to have larger CDR repertoires due to the evolutionary pressure of survival long enough to reproduce. Mice excel in their innate immunity but their adaptive immunity is not as robust as that of rabbits. This puts mice at a disadvantage for generating antibodies with high affinities.

Polyclonal antibody production

Affinity++Hit&miss
CDR Pool+++Large
Cost+Very low
Scalability+Low
Turnaround+++Fast
UseResearch, stepping stone for rabbit monoclonal

A Quick and cheap way to make some antibodies

Long story short, polyclonal antibodies work for research but not for diagnostics. It is indeed a very low cost and fast turn around method for making antibodies against certain targets. Boster Bio does offer a $600 package for making a polyclonal antibody. Some claim that polyclonal antibodies suffer from specificity because they originate from multiple clones. This is a misconception because this "disadvantage" can be mitigated by using a single epitope for immunization and purification of the polyclonal antibodies. There are 3 main disadvantages of polyclonal antibodies in our experience. First is lot-to-lot variability. For any clinical assays or therapeutic assays it is almost impossible to get approval for an assay with polyclonal antibodies as core components. For research use however it is acceptable, and is often used as a stepping stone towards a monoclonal antibody. In our experience most antigens that prove to generate a good polyclonal antibody can also make a good monoclonal antibody. The second disadvantage is its inability to amplify only the best clones. This is important if the target protein is difficult to detect and requires high affinity. This is often the case in detecting secretive proteins and biomarkers in liquid biopsies, via ELISA or other similar immunoassays. During affinity chromatography, all antibodies with enough affinity to bind to the very concentrated antigens will be pulled out. Many of these antibodies might not have enough affinity to bind to the target protein in vivo where the target protein exists in low levels and masked by competing binding receptors in the matrix/in vivo environment.

Phage display

Affinity++Can be better
CDR Pool+++Large
Cost+++High
Scalability+++High
Turnaround+Slow
UseDiagnotics & Theraputics

Best choice for diagnostic and Therapeutic antibodies

It lacks SOMATIC HYPERMUTATION. Even though phage display allows a reasonably large screening scale, it still falls short when compared to screening the full spleen, by at least a 1000 fold in scale. But the real problem is the CDR library phage display platforms have are "diamonds in the rough". Without going through SOMATIC HYPERMUTATION they will not be able to bind to the intended antigen with high affinity. Even though some claim they have secrete sauces in antibody engineering to improve antibody affinity after the fact, studies and our experience indicate that it is often no match to mother nature's affinity maturation process. It is a numbers game after all and the immune system can go through billions of iterations in vivo in a matter of weeks while as antibody engineering needs to experiment one amino-acid modification at a time, at weeks per iteration. Edited: some customers mentioned that one can train the phage library with certain antigen/pathogen groups to have better results. While this certainly helps, it is not a magic bullet to solve the intrinsic issues with phage display. I would also add that phage display does have an advantage, among many others, that it is great for generating antibodies against antigens that are not immunogenic or are autoimmunogenic, as it circumvents negative selection. See more information here Central Toloerance Wikipedia

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SERVICE DETAILS

Custom Rabbit Monoclonal Antibody Production Overview And Timeline.

Service overview

Boster's custom rabbit monoclonal antibody development platform uses Single Plasma Cell Interrogation Technology, which screens the immune repertoire of rabbits. Our aim is to produce an antibody that works for your specific application. Our technical staff will guide you through the project, from designing the antigen, immunization, screening strategy, antibody validation, to the proper handling of the plasmid that expresses your antibody. We can also generate antigens for you. Our antigen synthesis service cover both peptides and recombinant proteins. We are capable to express the antigen in E. coli, baculovirus, and mammalian cell lines.

The final deliverable is the sequences of selected positive clones, and plasmids containing the cloned antibody genes. Recombinant antibodies ensure the preservation of genetic information.

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Timeline and costs

Phase 1 Duration Deliverable
Peptide and Immunization: (2 rabbits) Titer Data
Peptide synthesis 10mg, >90% purity 1 week Titer Data
Peptide conjugation KLH for immunization and BSA-biotin conjugation for probing and screening 1 week Titer Data
Immunization for 2 rabbits 2-3 months Titer Data
Titer ELISA Assay Monitoring anti-sera titer and selecting the best animal for monoclonal
Phase 2 Duration Deliverable
First Screening with FACS (> a million B cells)
  1. Antigen-specific plasma cell identification and isolation.
  2. Single plasma cell heavy and light chain amplification
2 weeks
Supernatant production 0.25 ml scale, and Secondary screening with ELISA 1 week ELISA data
Phase 3 Duration Deliverable
Customer further screening with supernatant if necessary, choose three best clones.
Clone Deconvolution, cloning, expression/ELISA conformation; 30ml scale production, yield~ 0.5mg Ab per clone 3-4 week Sequences
Total 4-5 months

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Deliverables

What you will receive upon successful completion of above phases.

  • ELISA data from both sera and supernatants assays will be delivered.

  • Sequence information of the final delivered clones will be provided, as well as the map of the vector.

  • About 0.5 mg (0.1-1mg) of purified antibody from the two chosen clones to further testing.

  • About 100 ng of heavy and light chain plasmid DNA will be delivered for the final chosen clone.

  • A summary report will be provided

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FAQs

Q1. Does BosterBio manufacture antibodies in-house?

Yes. We have over 26 years of experience in developing antibodies and ELISA kits with our proprietary platform. Our antibodies and ELISA kits are manufactured in-house and rigorously tested to ensure high quality so that our products will work as expected for your experiments.

Q2. Will BosterBio update the antibody datasheet and/or COA if there are any changes?

Our online datasheets and COAs are not lot-specific. We provide the specific datasheets and COAs when we ship out antibodies. If you would like to request a lot-specific datasheet and/or COA, please contact [email protected] and provide the lot number.

Q3. Can I get a trial sample of the rabbit monoclonal antibody?

If available, an option for a 30ug trial sample size will be shown on the product page.

Q4. Can I get a discount for BosterBio antibodies?

We generally provide discounts for bulk orders. We also offer a “Buy Primary Antibody, Get Free HRP or Biotin Secondary for Free” promotion.

Q5. Which types of antibodies does BosterBio provide? How do I choose the right antibody for my experiment?

We provide primary antibodies and secondary antibodies. Our primary antibodies include rabbit polyclonal antibodies, rabbit monoclonal antibodies, and mouse monoclonal antibodies. We also offer several types of unconjugated and conjugated secondary antibodies. Selecting the right antibody is dependent on the application, specificity, cross-reactivity, species, and immunogen required for your experiment. If you have special conjugation requests or need any technical assistance, please contact [email protected].

Q6. Can BosterBio antibodies be used for multiple applications?

Our antibodies can be used for the validated applications listed on the product page. If the antibody does not work for a validated application shown on the product page, please contact [email protected]. Our Boster Guarantee states that if our technicians are unable to make your experiment work, we will offer a refund or replacement.

Q7. Will the antibody work for an application that is not listed on the product page?

Although an antibody may work for an application not shown on the product page, it is not covered by our Boster Guarantee. You can contact [email protected] to check if our lab has any data for the requested application.

Q8. Is the immunogen sequence provided?

We provide the immunogen sequence for most of our antibodies. If the immunogen sequence is not listed, please contact the [email protected] to check if the information can be shared.

Q9. What is the antibody clone ID? What is the difference between the clone ID and the lot number?

The antibody clone ID is a unique identity number assigned to each monoclonal antibody clone. The lot number is the identifier for each batch of antibodies produced.

Q10. Can BosterBio develop a custom rabbit monoclonal antibody for me if I do not find a suitable rabbit monoclonal antibody from the catalog?

If you cannot find a suitable rabbit monoclonal antibody from our catalog, please feel free to view details of our custom rabbit monoclonal antibody production service and contact us for a free consultation.

Q11. If the antibody does not work, can I request a replacement or refund?

If the antibody does not work for an application we have listed on the product page, it is covered by our Boster Guarantee. You can contact [email protected] for technical troubleshooting. If our technicians are unable to help make your experiment work, we will provide a replacement or refund.

Q12. What is the recommended concentration I should use for the primary antibody? How do I decide the suitable antibody dilution?

Recommended dilutions are listed on our product datasheets. However, the end user will need to determine the optimal dilution ratios because of different detection devices, methods, samples, and other factors.

Q13. Why is the actual band size different from the predicted band size for my western blot?

Please see this blog for possible reasons. If you have any questions, please contact [email protected].

Q14. When can I receive the products after I place an order??

For US customers, the lead time is typically 7-10 business days after you have placed your order. For international customers, please contact your distributor for time estimates. Custom rabbit monoclonal antibodies follow a different timeline. Please visit our custom rabbit monoclonal antibody service page and contact us for more details about the service and production timeline.

Q15. How do you ship antibodies?

We ship antibodies through FedEx Priority and provide the tracking number to you. The package will include wet ice.

Q16. How should I aliquot the antibodies?

Aliquot volumes are dependent on the amount needed for your experiment. However, we recommend aliquots should 10μL or more. If an aliquot has too little volume, antibody concentration may be affected by liquid evaporation and/or container adsorption.

Q17. How should I store the antibodies? What is the shelf life of BosterBio's antibodies?

Our rabbit monoclonal antibodies can be stored at -20°C for one year. After reconstitution, the rabbit monoclonal antibody should be stored at 4°C for one month. If aliquotted, the antibody can be stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.