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Spatial Biomarker Analysis for FFPE, Frozen Tissue, and Cell Pellet Samples

MULTIPLEX IHC SERVICES

Visualize multiple protein markers on the same tissue section to better understand tissue architecture, immune cell distribution, and biomarker co-expression.

  1. Panel design and antibody selection support
  2. Multiplex staining for spatial biomarker analysis
  3. Flexible support from pilot studies to larger sample sets

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About Boster Bio's Multiplex IHC Services

Boster has been manufacturing antibodies and reagents for pathology, IHC, and related immunoassay workflows since 1993. With decades of experience in antibody development, IHC reagent production, and sample testing services, Boster Bio supports researchers who need reliable staining, marker optimization, and spatial biomarker analysis.

Our multiplex IHC service extends Boster’s established IHC and histology service capabilities to projects that require multiple biomarkers to be visualized on the same tissue sample.

What studies can Multiplex IHC Services be used for?

  1. Tumor microenvironment studies
  2. Immune marker co-expression
  3. Cell-cell and cell-extracellular matrix interactions
  4. Spatial relationships between protein markers
  5. Tissue and organelle structure analysis
  6. Neuroscience research

What Is Multiplex IHC?

Multiplex immunohistochemistry, or multiplex IHC, enables the detection of multiple protein markers within a single tissue section. Compared with single-marker staining, multiplex IHC helps researchers evaluate marker co-expression, tissue architecture, immune cell localization, and spatial relationships between different cell populations.

This approach is especially useful for tumor microenvironment studies, immune-oncology research, biomarker discovery, neuroscience, and other projects where spatial context matters.

Biomarkers and Fluorophores

We offer a list of IHC-validated primary antibodies that may be suitable for multiplex IHC projects. You may also provide your own antibodies, provided they have been validated for IHC and are compatible with the planned staining workflow. If antibody validation or protocol optimization is needed, Boster can support this through our IHC validation and optimization service.

Need Antibody Validation First?

Fluorophore Options Available

  • DAPI (Nuclear)
  • Alexa Fluor 488
  • Alexa Fluor 555
  • Alexa Fluor 594
  • Alexa Fluor 647
  • Alexa Fluor 750

How To Work With Us?

Share your sample type, target markers, and project goals. Our team will help review panel feasibility before the project starts.

1. Get a Quote

2. Send Samples

3. Receive Results
Book a free assay design consultation

FAQs

As little as 1 sample can be submitted. However, we usually recommend sending enough sample for 5-10 assays when possible. More complex assays may need to be run in duplicate or triplicate depending on the project goal.

Please contact the Boster Bio service team. Our team can review your target markers, antibody options, sample type, and staining requirements before making project-specific suggestions.

Please review our guidelines on how to prepare and label samples before shipping them to us.

Services are available for the following types of samples:
  1. Formalin-fixed and paraffin-embedded (FFPE) tissues
  2. Frozen tissues (Cryo or OCT)
  3. Cell pellets

Please tell us:
  1. How many samples you would like to process
  2. What sample types you plan to use
  3. Whether you will provide the samples or need sourcing support
  4. Which target markers or antibodies you want to include
  5. Whether any antibodies need IHC validation or protocol optimization

We have validated analytes against many target species, including human, mouse, rat, porcine, canine, feline, and bovine. Please ask us for more details on available analytes and other species through the Boster Bio service team.

Yes. Multiplex IHC can be performed on fixed samples such as FFPE tissues, provided the sample quality is suitable and the antibodies are compatible with the staining workflow. If an antibody works in single-plex IHC, it may be a candidate for multiplex panel development, but panel compatibility still needs to be evaluated.

We typically recommend duplicate testing for method confidence and triplicate testing when stronger reproducibility is required. For small pilot studies, single testing may be appropriate depending on the project goal, sample availability, and budget. Our team can help recommend a testing plan based on your sample type, marker panel, and downstream analysis needs.

No fixed sample limit is listed for this service. Boster can support projects ranging from small pilot studies to larger sample sets. Please contact us with your sample number, marker panel, and timeline so we can recommend a practical workflow.

Sequential labeling and stripping conditions need to be optimized carefully because repeated staining cycles may affect tissue integrity, antigen accessibility, and signal quality. The practical panel size depends on the tissue type, marker selection, antibody performance, and staining workflow. Boster has experience with multi-marker panels and can help evaluate feasibility before the project starts.

About Boster Bio

PhD-level support
concierge-like service

Experience
since 1993

70,000+
publications

2000+
ELISA kits developed