Histopathology CRO Laboratory


Boster Bio offers IHC services with fast turnaround time. Whether you need small-scale research IHC service focusing on academic research and publishing, or transfer of a research assay to lab developed tests (LDT) and companion diagnostic tests (CDx), Boster Bio can help you achieve it with speed and low cost.

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Why Choose Boster For IHC CRO Service?

Because we are an antibody manufacturer with pathology focus

Since 1993, Boster has been manufacturing antibodies and reagents for pathology/IHC. Our founder came from a medical histology background and IHC has been in the company's gene from day 1. With hundreds of IHC experiments performed each week and after almost 3 decades of continuous improvement, Boster's technical team has mastered the art of IHC. Till end of 2019, there were more than 5,000 articles published using IHC results obtained from Boster antibodies (source: Google Scholar, Bioz and CiteAb). With our history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings with our IHC service.

"The rat brains were beautifully sectioned."

"Boster Bio performed a very thorough IHC validation. Ken was professional and accommodating throughout the project. We will come back to do some more work with them. "

"Provided superior quality IHC images."

"Their service is fast and the cost is reasonable."

Partners & Customers

Service Details

We optimize the antibody to find the best working dilution ratio. Then, we stain tissues and cells of interests on an automated system.

Phase 1: Antibody IHC Optimization Service

Finding the best dilution ratio for a completely new antibody

For a new antibody (i.e. antibody that has not been validated with IHC), we start off with the initial optimization service to determine the best epitope retrieval method and primary antibody incubation concentration. Having developed many different IHC assays for customers, we found that the following two strategies work quite well for most cases:

Multi-level IHC Validation

  • Use well characterized reference materials, such as cell lines and specific tissue types, for initial antibody screening and assessment.
  • Use carefully selected tissue arrays to further assess the sensitivity and specificity of antibodies and detection systems.
  • Validate on pilot study samples to ensure the robustness and consistency of the IHC assays.
Plan Standard Price Sample Epitope Retrieval Antibody Concentration
1 Inquire CPA or TMA Acidic ER: Citrate
Basic ER: EDTA or Tris/EDTA
5 concentration titrations (2-log):
• Mouse antibody: 5, 2.5, 1, 0.25, 0.05 μg/ml
• Rabbit antibody: 2, 1, 0.4, 0.1, 0.02 μg/ml
2 Inquire CPA or TMA Acidic ER: Citrate
Basic ER: EDTA or Tris/EDTA
3 concentration titrations:
2X, 1X, 0.5X of recommended concentration using an IHC tested antibody

If required, we will perform another round of optimization using Plan 2, starting with the selected concentration from Phase 1

*IF/ICC: We adopt similar principle in designing immunofluorescence (IF) and immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.

Phase 2: IHC staining on tissues of interest

using the antigen retrieval method and optimal concentration

We will then validate the antibody on sample sets using the conditions set in Phase 1. We can carry out the study automated on Leica Bond automated system or manually if requested. We offer cell pellet arrays (CPA) and tissue microarrays (TMA) that consist of different expression levels of the target of interest as the validation sample. To get the results in the most cost-effective way, we recommend customers to test on these arrays, because they can get the answer they want from a single slide (per testing condition).

Ready-to-use samples

Finding suitable samples is key to validating the specificity and sensitivity of the assay. We have in-house multi-human tissue arrays, both normal and tumor, for that purpose. We have a variety of samples for IHC development, including FFPE or frozen cell pellet arrays (CPA) and tissue microarrays (TMA) from human, mouse, rat, and etc. Our Special FFPE Cell Pellets Technique enables us to make cell smear slides with uniquely high homogeneity. Anecdotal feedback from our customers say it is the smoothest in the industry.

We can also accept sample slides provided by the customers.

Number of Slides 1 5 20+ 50+ 200+
Price Per Slide (USD) 60 60 55 55 Inquire

For using our samples instead of providing your own, please contact us for sample availability and pricing. We can also procure special samples (e.g. disease specific) for you as part of our procurement concierge service.

*IF/ICC: We adopt similar principle in designing immunofluorescence (IF) and immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.

Additional Details

  • Standard plans are 3 or 5 concentration titrations. If the antibody clone is completely new, we recommend 5 concentration titrations. However, if you roughly know the dilution to start with from another assay, such as western blot, 3 concentration titrations should be enough.
  • To request a quote, please let us know which samples you would like to validate the antibody on. Common samples are available at the lab. If you need uncommon wild type or pathological samples that are not available in our lab, there could be extra cost for acquiring them.
  • Various IHC assays using antibodies raised in species other than mouse and rabbit, such as goat and rat, have also been developed on both automated and manual staining platforms.
  • For all IHC studies, optional negative controls, such as isotype or no antibody controls, may be included
  • Boster Bio provides pathology review services by Board certified pathologists to help determine the sensitivity and specificity of IHC assays.
  • If you have any questions, please feel free to contact [email protected] to discuss your project.

Turnaround time

Depending on how many projects we have on hand, the turnaround time may vary from 1 week or more. Please contact us to get an accurate estimate of the turnaround time.

IHC image showcase

Below are some of the thousands of IHC images we've developed internally and for our customers