Histopathology CRO Laboratory

IHC SERVICES

IHC, IF/ICC, digital imaging, multiplex IHC service and more. Research grade CRO service, focusing on academic research and publishing.

Contact us for a quote

Why Choose Boster For IHC CRO Service?

Because we are an antibody manufacturer with pathology focus

Since 1993, Boster has been manufacturing antibodies and reagents for pathology/IHC. Our founder came from a medical histology background and IHC has been in the company's gene from day 1. With hundreds of IHC experiments performed each week and after almost 3 decades of continuous improvement, Boster's technical team has mastered the art of IHC. To end of 2019 there were more than 5000 articles published using IHC results obtained from Boster antibodies (source: Google scholar, Bioz and CiteAb). With such history and size, Boster is able to provide you a unique combination of service quality, subject expertise and cost savings, when it comes to IHC service.

Table Of Contents

Immunohistochemistry Service details

IHC Image Showcase

Available Specimens / Samples

Partners & Customers

Service details

First we optimize the antibody to find the best working dilution ratio. Then we stain tissues and cells of interests on an automated system

Phase 1: Antibody IHC optimization service

finding the best dilution ratio for a completely new antibody

For a new antibody, i.e. antibody that has not been validated with IHC, we start off with the initial optimization service to determine the best epitope retrieval method and primary antibody incubation concentration. Having developed many different IHC assays for customers, we found that the following two strategies works quite well for most cases:

Antibody procurement service

If you have not already purchased the antibodies, we can reach out to the antibody companies and procure them on your behalf. This is to further reduce the workflow for you to establish new SOPs with new antibodies.

Plan Standard Price Sample Epitope Retrieval Antibody Concentration
1 $450/antibody CPA or TMA Acidic ER: Citrate
Basic ER: EDTA or Tris/EDTA
5 concentration titrations (2-log):
• Mouse antibody: 5, 2.5, 1, 0.25, 0.05 μg/ml
• Rabbit antibody: 2, 1, 0.4, 0.1, 0.02 μg/ml
2 $270/antibody CPA or TMA Acidic ER: Citrate
Basic ER: EDTA or Tris/EDTA
3 concentration titrations:
2X, 1X, 0.5X of recommended concentration using an IHC tested antibody

If required, we will perform another round of optimization using plan 2, starting from selected concentration from Phase I

Price: $90 / slide including sectioning, staining, and digital scanning. Thus for plan 1 with 5 concentration titrations the total is $450, and for plan 2 with 3 concentration titrations the totla is $270. Bulk pricing please inquire.

*IF/ICC: We adopt similar principle in designing immunofluorescence (IF) and immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.

Phase 2: IHC staining on tissues of interest

using the antigen retrieval method and optimal concentration

We will then validate the antibody on sample sets using the conditions set in phase 1. We can carry out the study automated on Leica Bond automated system or manually if requested. We offer cell pellet arrays (CPA) and tissue microarrays (TMA) that consist of different expression levels of the target of interest as the validation sample. To get the results in the most cost-effective way, we recommend customers to test on these arrays, because they can get the answer they want from a single slide (per testing condition).

Ready to use samples

Finding suitable samples is key to validating the specificity and sensitivity of the assay. We have in-house multi-human tissue arrays, both normal and tumor, for that purpose. We have a variety of samples for IHC development, including FFPE or frozen cell pellet arrays (CPA) and tissue microarrays (TMA) from human, mouse, rat, and etc. Our Special FFPE Cell Pellets Technique enables us to make cell smear slides with uniquely high homogeneity. Anecdotal feedback from our customers say it is the smoothest in the industry.

We can also accept sample slides provided by the customers.

Price: $60 / sample for IHC on Leica Bond automated system. $10 / slide for scanning (only for positive slides, thus this is calculated separately.

For doing immunohistochemistry manually please inquire for quote.

Number of Slides 1 5 20+ 50+ 200+
Price Per Slide (USD) 60 60 55 55 Inquire

For using our samples instead of providing your own, please contact us for sample availability and pricing. We can also procure special samples (disease specific, etc.) for you as part of our procurement concierge service.

*IF/ICC: We adopt similar principle in designing immunofluorescence (IF) and immunocytochemistry (ICC) assays. Please inquire for IF/ICC pricing.

Additional Details

  • Standard plans are 3 or 5 concentration titrations. If the antibody clone is completely new, we recommend 5 concentration titrations. However, if you roughly know the dilution to start with from another assay, such as western blot, 3 concentration titrations should be enough.
  • To request a quote, please let us know which samples you would like to validate the antibody on. Common samples are available at the lab. If you need uncommon wild type or pathological samples that are not available in our lab, there could be extra cost for acquiring them.
  • Pricing: The initial optimization of your antibody will be done manually at $90/sample per condition. For example, if Plan 1 is chosen for one type of sample (recommended), the price would be $450. Once your antibody is IHC optimized, our cost is $60 per sample for performing IHC using an automated Leica Bond system and $10 for whole slide scanning (optional). We also have a tissue/cell smear array option available if you want to validate the antibody on multiple tissues at once.
  • Various IHC assays using antibodies raised in species other than mouse and rabbit, such as goat and rat, have also been developed on both automated and manual staining platforms.
  • For all IHC studies, optional negative controls, such as isotype or no antibody controls, may be included
  • Bosterbio provides pathology review services by Board certified pathologists to help determine the sensitivity and specificity of IHC assays.
  • If you have any questions, please feel free to contact [email protected] to discuss your project.

Turn around time

Depending on how many projects we have on hand, the turn around time may very from 1 week to more. Please contact us to get an accurate estimate of turn around time.

IHC image showcase

below are some of the tens of thousand IHC images we developed internally and for our customers

Available Specimens / Samples

Human Specimens In FFPE Blocks

Bladder Cancer Human Breast Cancer Colorectal Cancer With Total RNA Lung Cancer With Total RNA
Brain Cancer Head and neck Cancer Esophagus Cancer With Total RNA Lymphoma Cancer With Total RNA
Breast Cancer Bladder Cancer With Total RNA Head and neck Cancer With Total RNA Multiple myeloma Cancer With Total RNA
Cervical Cancer Brain Cancer With Total RNA Kidney Cancer With Total RNA Neuroblastoma Cancer With Total RNA
Colorectal Cancer Breast Cancer With Total RNA Leukemia Cancer With Total RNA Ovarian Cancer With Total RNA
Esophagus Cancer Cervical Cancer With Total RNA Liver Cancer With Total RNA Pancreatic Cancer With Total RNA