Boster’s Monoclonal Antibody Production Service
Boster offers a three-stage custom rat and mouse monoclonal antibody production service for a promotional price of $4,299. This article provides the technical details regarding the services included in each stage.
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Overview: we make antibody that works for your application
In vivo antibody production is a relatively unpredictable process. It is important to work with experienced experts as some small details are easily overlooked but can cause the project to fail. Our experienced monoclonal antibody production team will consult with you throughout the process to mitigate the risks of monoclonal antibody production. Our goal is not only to make an antibody that recognizes the antigen but to make an antibody that works for your specific application.
We consult on validation process before starting the project
Sera screening is one of the critical step in the monoclonal antibody production process. While ELISA and Western Blotting are included in the standard package, we can work with you to design additional screening method specific to your application of interest. We will also assist you in determining the positive and negative controls for the validation process.
First stage includes immunization and sera screening
We can use a variety of antigen types including but not limited to peptides and recombinant proteins. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us. Once the antigen is determined, the first stage will begin with immunizing Balb/C mice and ELISA screening of polyclonal sera. You can add additional screening criteria into this step. The sera exhibiting highest titer will be used for fusion in the second stage.
Second stage includes fusion, subcloning and application-specific screening
In the second stage we will fuse the high titer splenocytes with mouse myeloma cells and isolate the antigen positive hybridoma cells. In this step we will eliminate the failed fusions of splenocyte-to-splenocyte, myeloma-to-myeloma and unfused cells. After this first elimination there are usually 3000 successful clones left for an ELISA screening for antigen positive clones. This will further narrow the candidate pool to around 10 clones for subcloning. Before subcloning, we will send the supernatant of 2-3 of the ELISA positive clones to you for your evaluation.
Final stage includes expansion of satisfactory hybridoma clones
Once you select two clones out of the final candidate pool, we will isolate these clones of interests using the cloning by limiting dilution method. We will expand these clones of interests into monoclonal colonies which are then ready for cryopreservation. We will retain 2 vials of each hybridoma for you for 6 months. You can purchase additional storage services for a small fee.
Shipping and handling details for hybridoma
We will send you frozen vials of hybridoma cells. Live hybridoma cultures in T25 flasks can also be sent upon request within USA and internationally if there are no import restrictions.
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Additional related services
See the table below for additional services and their prices.
|Peptide||Peptide synthesis and conjugation||$500||Peptide synthesis without conjugation||$350||Peptide conjugation||$150|
|Screening||Additional clones (>10) in Phase II||$50 per clone||Negative screening||$300||Extra screening with IHC||$300||Additional clones (>2) in Phase III||$300 per clone||Cell-line adaptation to serum free medium||$500~1,000|
|Ascites||Ascites production (<11 mice )||$30/mouse+$200 setup fee||Ascites production (11-50 mice)||$26/mouse+$200 setup fee||Ascites production (51-99 mice )||$24/mouse+$200 setup fee||Ascites production (>99 mice )||$20/mouse+$200 setup fee|
|Purification||Antibody purification||$30/mouse||Antibody culture in bioreactor, per liter||$1,100||Antibody culture in flask, per liter||$800||Antibody purification by protein G from one liter of medium||$300||Antibody purification by protein G from ascites (20mg)||$300|
|Labeling||Antibody conjugation with FITC, per mg||$600||Antibody conjugation with HRP, per mg||$600||Antibody biotinylation, per mg||$600|
|Other||Cell banking and storage of 10 ampules after first two months||$30/month||Antibody epitope mapping by peptide library||$1,200|