Custom Monoclonal Antibody Production Service

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Service Packages Pricing

Package 1

$4000

User Provide Antigen

The most economical solution on the market for custom monoclonal antibody using user provided antigen. Basic screening via direct ELISA.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 3 mice
  • Sera screening via ELISA
  • Sera screening via other methods
  • Fushion and sub-cloning
  • Supernatant Screening via ELISA
  • Supernatant Screening via other methods
  • Clone selection and expansion
  • Antibody production (1 mouse)
  • Antibody purification (1 mouse)
  • Shipping and handling
  • Deliverables:
  • Anti-sera test report
  • Supernatant test report
  • Purified Monoclonal Antibodies
  • 2 positive clones
  • 1mg+ recombinant protein
  • VIP status at Bosterbio.com

Package 2

$4500

Boster Provide Antigen

Do not have antigen protein? No worries. We will synthesize a recombinant protein for you for only $500.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 3 mice
  • Sera screening via ELISA
  • Sera screening via other methods
  • Fusion and sub-cloning
  • Supernatant Screening via ELISA
  • Supernatant Screening via other methods
  • Clone selection and expansion
  • Antibody production (1 mouse)
  • Antibody purification (1 mouse)
  • Shipping and handling
  • Deliverables:
  • Anti-sera test report
  • Supernatant test report
  • Purified Monoclonal Antibodies
  • 2 positive clones
  • 1mg+ recombinant protein
  • VIP status at Bosterbio.com

Package 3

Custom

Fully Customizable

Contact us for a free consultation. See details in Additional Services. We will try our best to accommodate all of your custom requests.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 3 mice
  • Sera screening via ELISA
  • Sera screening via other methods
  • Fushion and sub-cloning
  • Supernatant Screening via ELISA
  • Supernatant Screening via other methods
  • Clone selection and expansion
  • Antibody production (1 mouse)
  • Antibody purification (1 mouse)
  • Shipping and handling
  • Deliverables:
  • Anti-sera test report
  • Supernatant test report
  • Purified Monoclonal Antibodies
  • 2 positive clones
  • 1mg+ recombinant protein
  • VIP status at Bosterbio.com
*Boster Fail Safe for Monoclonal Service

If a successful clone cannot be found, customer has the following options:

1. Cancel the project after the first delivery of supernatants and only be charged $1,000 for package 1 and $1,500 for package 2 (will still receive recombinant proteins).

2. Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, customer may choose to cancel the project and only be charged $1,000/$1,500 plus the cost of the additional 10 supernatants, for a total of $1,999/$2,499

Project Timeline

Boster Guarantee

Expertise

Team of experts with decades of combined experience dedicated to producing your monoclonal antibody.

Communication

A dedicated point of contact for you throughout the monoclonal project. Weekly project updates available.

Validation

Basic validation via direct ELISA. Advanced application-specific validation available upon request.

Service Details

Phase Description/Timeline Deliverables Options/Additional Services
Phase I

Immunization
Timeline: 6 weeks.
Requirements: 3-5 mg of antigen, or immunogen sequence

We can use a variety of antigen types including but not limited to peptides and recombinant proteins. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us. Once the antigen is determined, the first stage will begin with immunizing Balb/C mice and ELISA screening of polyclonal sera. You can add additional screening criteria into this step. The sera exhibiting highest titer will be used for fusion in the second stage.
Test bleed report. Optional: we can also provide 15ul of anti sera for you to test. 1. Antigen synthesis, we make recombinant protein for additional $500. The recombinant protein will be used as immunogen. Purified recombinant protein will be provided to the customer at end of the project (value $2000).
2. Immunizing additional mice. Details see Additional Services below.
Phase II

Cell Fusion and Screening
Timeline: 7-8 weeks.

Fuse the high titer splenocytes with mouse myeloma cells, grow cells in HAT medium and isolate the antigen positive hybridoma cells. In this step we will eliminate the failed fusions of splenocyte-to-splenocyte, myeloma-to-myeloma and unfused cells. After this first elimination there are usually 3000 successful clones left for an ELISA screening for antigen positive clones. This will further narrow the candidate pool to around 10 clones for subcloning. Before subcloning, we will send the supernatant of 2 of the ELISA positive clones to you for your evaluation.
Cell cuture testing report. 1ml supernatant of each selected clone. 1. additional clone screening (in addition to the default 10).
2. additional screening criteria. Pricing see Additional Services below.
Phase III

Subcloning, Expansion and Delivery
Timeline: 6-7 weeks

Once you select two clones out of the final candidate pool, we will isolate these clones of interests using the cloning by limiting dilution method. We will expand these clones of interests into monoclonal colonies which are then ready for cryopreservation. We will produce a small batch of monoclonal antibodies using 3 mice via ascites production and purify the antibodies via protein A.
Protein A purified monoclonal antibody 1-3mg per clone; 2 hybridoma clones; 6 months of cell banking service. 1. additional clone for subcloning (in addition to the default 2).
2. antibody production and purification
3. antibody conjugation
4. hybridoma sequencing
5. additional cell banking
6. expedited shipping and handling for hybridomas

Additional Services

Recombinant Protein

$500

Instead of providing us the immunogen, we make a recombinant protein for your target of interest. You will receive 1mg+ of purified recombinant protein as well. Total value: $1500. This service takes 1 additional month.

Custom Screening Criteria

Contact us for pricing

We can conduct additional screening methods per your requirement. Such as testing anti/sera and or culture supernatants in chosen samples on WB, IHC, Flow etc.

Immunizing More Mice

$80/mouse

In phase I, immunize more mice than the default 3. This could increase the chance of having at least one strong positive mouse.

Additional Clones

$250/clone

In phase II, select more clones than the default 10. In phase III, keep more clones than the default 2, $250 for each additional clone.

Antibody Production

$200 setup fee + $30/mouse

Ascites antibody production for a chosen clone. For 1-10 mice, $30/mouse, for 10+ mice, $20/mouse. Ascites from these productions will be delivered. On average one mouse produces 2-4ml ascites, 1ml ascites contain 1mg+ monoclonal antibodies.

Antibody Purification

$30/mouse

Protein A purify the monoclonal antibodies. This service is included in your package for 1 ascites antibody production and can be purchased for additional antibody productions.

Antibody Conjugation

$600/mg

For purified monoclonals only. Available conjugates: FITC, TRITC, Cy3, DyLight®488, Colloidal Gold (5nm/10nm), Biotin, HRP.

Cell Banking

$30/month*clone

Storage service for your clones beyond the 6 months of free cell banking service which is included in the package.

Hybridoma Sequencing

$800/clone

Cloning and sequencing hybridoma antibody heavy and light chain variable regions.

About Boster Biological Technology

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