Custom Monoclonal Antibody Production Service

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Service Packages Pricing

Package 1

$4000

User Provides Antigen

The most economical solution on the market for custom monoclonal antibody using user-provided antigen. Basic screening via indirect ELISA.

  • boster_tick Dedicated project manager
  • boster_tick Weekly updates on project status
  • red-x Antigen synthesis
  • boster_tick Immunizing 3 Balb/C mice
  • boster_tick Anti-sera test via indirect ELISA
  • red-x Anti-sera test via other methods
  • boster_tick Fusion and sub-cloning
  • boster_tick Positive clone screening via indirect ELISA
  • red-x Positive clone screening via other methods
  • boster_tick Positive clone selection and expansion
  • boster_tick Antibody production via ascites of 1 Balb/C mouse
  • boster_tick Antibody purification via affinity purification
  • boster_tick Shipping and handling
  • Deliverables:
  • boster_tick Anti-sera test report
  • boster_tick Supernatant test report
  • boster_tick 2 purified monoclonal antibodies
  • boster_tick 2 positive clones
  • red-x 1mg+ antigen (recombinant protein or peptide)
  • boster_tick VIP status at Bosterbio.com

Package 2

$4500

Boster Provides Antigen

Do not have antigen protein? No worries. We will synthesize the antigen for you for only $500.

  • boster_tick Dedicated project manager
  • boster_tick Weekly updates on project status
  • boster_tick Antigen synthesis
  • boster_tick Immunizing 3 Balb/C mice
  • boster_tick Anti-sera test via indirect ELISA
  • red-x Anti-sera test via other methods
  • boster_tick Fusion and sub-cloning
  • boster_tick Positive clone screening via indirect ELISA
  • red-x Positive clone screening via other methods
  • boster_tick Positive clone selection and expansion
  • boster_tick Antibody production via ascites of 1 Balb/C mouse
  • boster_tick Antibody purification via affinity purification
  • boster_tick Shipping and handling
  • Deliverables:
  • boster_tick Anti-sera test report
  • boster_tick Supernatant test report
  • boster_tick 2 purified monoclonal antibodies
  • boster_tick 2 positive clones
  • boster_tick 1mg+ antigen (recombinant protein or peptide)
  • boster_tick VIP status at Bosterbio.com

Package 3

Custom

Fully Customizable

Contact us for a free consultation. See details in Additional Services. We will try our best to accommodate all of your custom requests.

  • boster_tick Dedicated project manager
  • boster_tick Weekly updates on project status
  • boster_tick Antigen synthesis
  • boster_tick Immunizing 3 Balb/C mice
  • boster_tick Anti-sera test via indirect ELISA
  • boster_tick Anti-sera test via other methods
  • boster_tick Fusion and sub-cloning
  • boster_tick Positive clone screening via indirect ELISA
  • boster_tick Positive clone screening via other methods
  • boster_tick Positive clone selection and expansion
  • boster_tick Antibody production via ascites of 1 Balb/C mouse
  • boster_tick Antibody purification via affinity purification
  • boster_tick Shipping and handling
  • Deliverables:
  • boster_tick Anti-sera test report
  • boster_tick Supernatant test report
  • boster_tick 2 purified monoclonal antibodies
  • boster_tick 2 positive clones
  • boster_tick 1mg+ antigen (recombinant protein or peptide)
  • boster_tick VIP status at Bosterbio.com


Boster Fail-safe for Monoclonal Service

If immunization fails, the customer has the following options:

1. Cancel the project after the delivery of the anti-sera test report and only be charged $500 for Package 2. The customer will still receive 1mg+ antigen.

2. Restart the immunization for free and continue the project.


If a successful clone cannot be found, the customer has the following options:

1. Cancel the project after the first delivery of supernatants and only be charged $1,000 for Package 1 and $1,500 for Package 2 (will still receive 1mg+ antigen).

2. Purchase an additional 10 supernatants for $999. If a successful clone cannot be found after the second supernatant delivery, the customer may choose to cancel the project and only be charged $1,000/$1,500 plus the cost of the additional 10 supernatants, for a total of $1,999/$2,499

Feel free to read about our monoclonal validation process here: Monoclonal Validation Information

We respect our customer's confidential information and guarantee that all intellectual property remains with the customer.

Project Timeline

Milestone Duration
Plasmid Synthesis 14 days
Antigen Expression 10 days
Immunization First Shot 3 weeks

*Time interval between the first immunization and the second immunization
Immunization Second Shot 2 weeks

*Time interval between the second immunization and the third immunization
Immunization Third Shot 2 weeks

*Time interval between the third immunization and the fourth immunization
Immunization Fourth Shot 5 days

*Test bleed on the fifth day after the fourth shot
Fusion & Screening (Customer Testing) 3-4 weeks

Deliverables:Test bleed report"
Subcloning 3 weeks

Deliverables: Test report"
Expansion & Purification 4-5 weeks
Deliver Reports 1 Day

Deliverables: Protein A purified monoclonal antibody 1-3mg per clone; 2 hybridoma clones; 6 months of cell banking service

*Shipping may take 7-10 business days

Boster Guarantee

Expertise icon

Expertise

Team of experts with decades of combined experience dedicated to producing your monoclonal antibody.

Communication

A dedicated point of contact for you throughout the monoclonal project. Weekly project updates available.

Validation

Basic validation via indirect ELISA. Advanced application-specific validation available upon request.

Service Details

Phase Description/Timeline Deliverables Options/Additional Services
Phase I (Optional)

Antigen Production

Price: $500
Boster Provides Antigen
Timeline: 4 weeks
The customer will provide the sequence and we will generate the antigen from the provided information.

User Provides Antigen
Timeline: 3 days (Process Antigen)
Requirements: 3-5 mg of antigen. For the immunization antigen (IA), the concentration should be at least 1.0mg/mL and the amount needed is 2mg. For the screening antigen (SA), the concentration should be at least 1.0mg/mL and the amount needed is 1mg.

We can use a variety of antigen types, including, but not limited to, peptides and recombinant proteins. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us.
1mg+ antigen (recombinant protein or peptide) The antigen will be provided to the customer at the end of the project. If the customer provides the antigen, this phase is omitted.
Phase II

Immunization

Price: $500 (2 attempts)
Timeline: 7 weeks
Once the antigen is determined, the first stage will begin with immunizing 3 Balb/C mice and ELISA screening of polyclonal sera. You can add additional screening criteria into this step. The sera exhibiting the highest titer will be used for fusion in the third phase.
Anti-sera test report Immunizing additional mice. For details, please see "Additional Services" below.
Optional: We can also provide 15ul of anti-sera for the customer to test.
Phase III

Cell Fusion and Screening

Price: $700
Timeline: 3 - 4 weeks

Fuse the high titer splenocytes with mouse myeloma cells, grow cells in HAT medium and isolate the antigen positive hybridoma cells. In this step, we will eliminate the failed fusions of splenocyte-to-splenocyte, myeloma-to-myeloma, and unfused cells. After this first elimination, there are usually 3000 successful clones left for an ELISA screening for antigen positive clones. This will further narrow the candidate pool to around 10 clones for subcloning. Before subcloning, we will send the screening report of cell fusion supernatants to you for your evaluation.
Screening report of cell fusion supernatants Additional screening criteria. For pricing, please see "Additional Services" below.
Phase IV

Subcloning

Price: $1000
Timeline: 3 weeks

In this phase, we will further narrow the candidate pool to around 10 clones for subcloning by limiting dilution method. Subcloning will be operated two times or more until all the cell supernatants are positive. After subcloning, we will send the supernatant of 2-3 positive clones to you for your evaluation.
Subcloning test report of antigen positive hybridoma cells 1. 1ml supernatant of 2-3 selected clones.
2. Additional clones for subcloning (in addition to the default 2).
Phase V

Expansion, Purification, and Delivery

Price: The remaining cost of the project
Timeline: 4 - 5 weeks
Delivery time: 1 - 2 weeks

Once you select two clones out of the final candidate pool, we will expand these clones of interests into monoclonal colonies which are then ready for cryopreservation. We will produce a small batch of monoclonal antibodies using 1 mouse via ascites production and purify the antibodies via protein A.
Protein A purified monoclonal antibody 1-3mg per clone;
2 hybridoma clones;
6 months of cell banking service
1. Antibody production and purification (in addition to the default 2)
2. Antibody conjugation
3. Hybridoma sequencing
4. Additional cell banking
5. Expedited shipping and handling for hybridomas

Additional Services

Antigen Synthesis

$500

Instead of providing us the immunogen, we synthesize antigen for your target of interest. You will also receive 1mg+ of purified antigen (recombinant protein or peptide). Total value: $1500. This service takes 1 additional month.

Custom Screening Criteria

Contact us for pricing

We can conduct additional screening methods per your requirement, such as testing anti-sera and/or culture supernatants in chosen samples on WB, IHC, Flow, etc.

Immunizing More Mice

$80/mouse

In Phase II, we will immunize more mice than the default 3. This could increase the chance of having at least one strong positive mouse.

Additional Clones

$250/clone

In Phase III, select more clones than the default 10. In Phase IV, keep more clones than the default 2, $250 for each additional clone.

Antibody Production

$200 setup fee + $30/mouse

Ascites antibody production for a chosen clone. For 1-10 mice, $30/mouse. For 10+ mice, $20/mouse. Ascites from these productions will be delivered. On average, one mouse produces 2-4ml ascites, 1ml ascites contain 1mg+ monoclonal antibodies.

Antibody Purification

$30/mouse

Protein A purification of the monoclonal antibodies. This service is included in your package for 1 ascites antibody production and can be purchased for additional antibody productions.

Antibody Conjugation

$600/mg

For purified monoclonals only. Available conjugates: FITC, TRITC, Cy3, DyLight®488, Colloidal Gold (5nm/10nm), Biotin, HRP.

Cell Banking

$30/month*clone

Storage service for your clones beyond the 6 months of free cell banking service, which is included in the package.

Hybridoma Sequencing

$800/clone

Cloning and sequencing hybridoma antibody heavy and light chain variable regions.

About Boster Biological Technology

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