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Anti-CSF1 Antibody (A00620-3) produced distinct target bands in WB assays using mouse macrophages, with only faint non-specific bands observed and consistent GAPDH loading control.

Excellent, submitted by on
SKU A00620-3
Application Western Blot
Sample Mouse macrophages
Sample Processing Description Cells were lysed in RIPA lysis buffer supplemented with PMSF (100:1) for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected and mixed with 5× Loading Buffer. Samples were denatured at 100°C for 10 min and then loaded onto an SDS-PAGE gel.
Other Reagents Blocking buffer: 5% non-fat milk
Primary Antibody CSF1 Antibody Picoband®
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:2000, incubated for 1 hour at RT.<
Detection ECL, Imaging system: UNITEC CAMBRIDGE
Results Summary The figure displays representative Western blot results for the target protein CSF1 and the internal control GAPDH in different types of macrophages; the target bands are clear and distinct, and the experimental results are satisfactory.

Anti-STAT3 Antibody (M00007) successfully revealed downregulated STAT3 expression in liver tissues of ANIT-induced cholestatic rats via Western blot, accompanied by minor non-specific bands.

Excellent, submitted by on
SKU M00007
Application Western Blot
Sample Liver tissue from an ANIT-induced rat model
Sample Processing Description Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents Blocking buffer, RIPA lysis buffer
Primary Antibody STAT3 Rabbit Monoclonal Antibody
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000, Incubate for 1 hour at room temperature.
Detection ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary The image shows the following lanes: Lane 1 represents the liver of a control group rat, displaying a clear and uniform STAT3 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, showing a markedly reduced STAT3 band. This demonstrates that the antibody effectively detects changes in STAT3 expression.

Anti-JAK2 Antibody (M00027) reliably detected reduced JAK2 abundance in liver tissue from ANIT-induced cholestatic rats by WB, with only faint non-specific bands present.

Excellent, submitted by on
SKU M00027
Application Western Blot
Sample Liver tissue from an ANIT-induced rat model
Sample Processing Description Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents Blocking buffer, RIPA lysis buffer
Primary Antibody JAK2 Monoclonal Antibody
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000
Detection ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary The image shows the following lanes: Lane 1 represents the liver of a control group rat, displaying a clear and uniform JAK2 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the JAK2 band is markedly reduced. This demonstrates that the antibody can effectively detect changes in JAK2 expression.

Anti-HO-1/HMOX1 Antibody (PB9085) tracked the downregulation of HO-1/HMOX1 in ANIT-induced cholestatic rat liver via WB, with subtle non-specific bands and consistent GAPDH loading control.

Excellent, submitted by on
SKU PB9085
Application Western Blot
Sample Liver tissue from an ANIT-induced rat model
Sample Processing Description Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents Blocking buffer, RIPA lysis buffer
Primary Antibody Heme Oxygenase 1/HMOX1 Antibody Picoband®
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000
Detection ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary The image shows the following lanes: Lane 1 represents the liver of control group rats, displaying a clear and uniform HO-1/HMOX1 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the HO-1/HMOX1 band is markedly reduced. Expression of the internal control GAPDH remains consistent. These results demonstrate that the antibody effectively detects changes in HO-1/HMOX1 expression.

Anti-GPX4 Antibody (A02059-1) reliably detected the upregulation of GPX4 in liver tissues from ANIT-induced cholestatic rats by WB, with only faint non-specific bands observed.

Excellent, submitted by on
SKU A02059-1
Application Western Blot
Sample Liver tissue from an ANIT-induced rat model
Sample Processing Description Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents Blocking buffer, RIPA lysis buffer
Primary Antibody COL6A1 Antibody Picoband®
Primary Incubation Glutathione Peroxidase 4/GPX4 Antibody Picoband® GPX4 antibody
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000
Detection ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary The image shows the following lanes: lanes 1 and 4 represent liver samples from control group rats, displaying clear and uniform GPX4 bands; lanes 2 and 5 represent the ANIT-treated group; and lanes 3 and 6 represent the drug-treated group, showing markedly increased GPX4 bands. These results demonstrate that the antibody effectively detects changes in GPX4 expression.

Anti-CYP7A1 Antibody (RP1079) successfully captured CYP7A1 expression reduction in liver samples from ANIT-induced cholestatic rats via WB, with only faint off-target bands visible.

Excellent, submitted by on
SKU RP1079
Application Western Blot
Sample Liver tissue from an ANIT-induced rat model
Sample Processing Description Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents Blocking buffer, RIPA lysis buffer
Primary Antibody COL6A1 Antibody Picoband®
Primary Incubation
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000
Detection ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary The image shows the following lanes: Lane 1 represents the liver of control-group rats, displaying a clear and uniform CYP7A1 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the CYP7A1 band is markedly reduced. This demonstrates that the antibody effectively detects changes in CYP7A1 expression.

Anti-ABCB4 Antibody (PB9275) clearly detected ABCB4 expression shifts in liver tissue from ANIT-induced cholestatic rats by WB, with only faint non-specific bands observed.

Excellent, submitted by on
SKU PB9275
Application Western Blot
Sample ANIT Model Rat Liver Tissue
Sample Processing Description SD Rat Bile Stasis Liver Injury Induced by ANIT Gavage: Liver tissue was collected 48 h after induction, proteins were extracted using RIPA lysis buffer, quantified using a BCA Protein Assay Kit, and denatured by boiling after addition of Loading Buffer.
Other Reagents Blocking Solution; RIPA Lysis Buffer
Primary Antibody ABCB4 Antibody Picoband®
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:50000
Detection ECL, Imaging system: Contact-based non-destructive imaging
Results Summary The image shows the following lanes: Lane 1 represents the liver of a control group rat, displaying a clear and uniform ABCB4 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, showing a markedly increased ABCB4 band. This demonstrates that the antibody can effectively detect changes in ABCB4 expression.

Anti-TUBB Antibody (A01857-1) produced distinct green fluorescent signals with low background in 400× co-staining (FISH + IF) of HepG2 cells, showing precise localization of beta-tubulin within the cytoskeleton.

Excellent, submitted by on
SKU A01857-1
Application Immunocytochemistry / Immunofluorescence
Sample Human HepG2 cells
Sample Processing Description Human HepG2 cells on coverslips were treated with pepsin at room temperature for 10 minutes, followed by antigen retrieval using a composite digestion solution for 10 minutes.
Other Reagents Mixed Enzyme Digestion Solution; DAPI Staining Solution
Primary Antibody Beta Tubulin/TUBB Antibody Picoband®
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Fluoro 488-Goat Anti-Rabbit (H+L) (Boster BA1127)
Secondary Incubation Incubate at 37°C for 30 minutes
Detection Imaging system: Leica DMi3000 Inverted Fluorescence Microscope
Results Summary This image shows the results of co-staining for SIRT1 mRNA (via FISH) and beta-tubulin (via IF) at 400x magnification; green fluorescence indicates positive beta-tubulin staining, while red indicates the mRNA FISH signal. Beta-tubulin is a major component of microtubules, one of the three primary fibrous systems constituting the cytoskeleton. Microtubules are essential for maintaining cell morphology and facilitating processes such as intracellular transport, cell division, and cell motility; the co-staining results demonstrate clear visualization and precise localization within the cytoskeleton.

Anti-GAPDH Antibody (A00227-1) generated a sharp, clean band around 36 kDa with no non-specific signals in WB using human SHSY5Y1 cells, confirming its high specificity as a reliable loading control.

Excellent, submitted by on
SKU A00227-1
Application Immunohistochemistry
Sample Human SH-SY5Y cells
Sample Processing Description Extract total cellular protein, denature it with loading buffer, and load 10 µg of the protein sample.
Other Reagents Blocking solution (5% non-fat milk); BCA Protein Assay Kit
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit IgG HRP-conjugated secondary antibody (BA1054)
Secondary Incubation Incubate at room temperature for 2 hours.
Detection ProteinSimple FluorChem system (BIO-RAD, ChemiDocTM XRS+)
Results Summary The human SH-SY5Y cell line was used for this experiment. The resulting GAPDH protein band appeared at approximately 36 kDa; the band was distinct and showed stable expression with no non-specific bands. These results further confirm the antibody's excellent recognition capability and high specificity, aligning with expectations.

Anti-TXNIP Antibody (A01409-1) delivered specific IHC staining with low background and confirmed weaker TXNIP expression in human colorectal carcinoma versus adjacent non-tumor tissues.

Excellent, submitted by on
SKU A01409-1
Application Immunohistochemistry
Sample Human colorectal cancer and adjacent non-tumor tissue samples
Sample Processing Description ① Human colon cancer tissue ② Human adjacent non-tumor colon tissue
Other Reagents Goat serum, Two-Step Histochemistry Kit
Primary Antibody TXNIP Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-Step Histochemistry Kit
Secondary Incubation Incubate at 37°C for 45 minutes
Detection Imaging system: Leica DM2500
Results Summary TXNIP is widely recognized as an important tumor suppressor gene; it is downregulated in most cancers, and its expression level is significantly correlated with patient prognosis. Experimental results have also confirmed this.
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