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Anti-CD326/EPCAM Antibody (M30950-1) specifically detected EPCAM (~34 kDa) in HepG2 cells by Western blot, producing clean and stable target bands without obvious non-specific binding, which validates its high specificity and reliable performance.

Excellent, submitted by on
SKU M30950-1
Application Western Blot
Sample Human HepG2 cell lysates
Sample Processing Description Following lentiviral transduction, the HepG2 cell line was harvested by centrifugation. The cell pellet was lysed using enhanced RIPA buffer, denatured in 5× SDS loading buffer at 98°C for 8 minutes, and then loaded onto an SDS-PAGE gel.
Other Reagents Enhanced RIPA Lysis Buffer (Catalog No. AR0102, Boster Biological Technology)
Primary Antibody EpCAM/Trop1 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody (Catalog No. BA1054, Boster Biological Technology)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:MiniChemi 500
Results Summary In this experiment, the HepG2 cell line was used as the sample. The resulting EPCAM protein band appeared at approximately 34 kDa; it was clear, showed stable expression, and exhibited no significant non-specific binding. These results further confirm that the Anti-CD326/EPCAM antibody possesses excellent recognition capability and high specificity, consistent with the expected experimental outcomes.

Anti-UCP1 Antibody (M00255) yielded well-resolved target bands with marked intergroup expression differences in WB using mouse brown adipose tissue, and ACTB served as loading control.

Excellent, submitted by on
SKU M00255
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody UCP1+UCP3 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot results for the target protein UCP1 and the internal control ACTB across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-SOX9 Antibody (M00177) produced distinct bands and notable expression differences across groups in WB of mouse brown adipose tissue, with GAPDH as loading control.

Excellent, submitted by on
SKU M00177
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody SOX9 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot results for the target protein SOX9 and the internal control GAPDH across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-PLIN1 Antibody (M03918) produced clear target bands and prominent expression differences among groups in WB of mouse brown adipose tissue, with ACTB as loading control.

Excellent, submitted by on
SKU M03918
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody Perilipin A PLIN1 Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot results for the target protein PLIN1 and the internal control ACTB across different experimental groups. The target bands are clear and well-defined, with significant differential expression observed between the experimental groups; the results are satisfactory.

Anti-PGC1A Antibody (M00236) yielded distinct bands with obvious expression differences across groups in WB using mouse adipocytes, with ACTB as loading control.

Excellent, submitted by on
SKU M00236
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody PGC1 alpha PPARGC1A Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot results for the target protein PGC1A and the internal control ACTB across different experimental groups. The target bands are clear and well-defined, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-EBF2 Antibody (A00282-3) produced sharp target bands and notable intergroup expression differences in WB assays with mouse adipocytes, using ACTB as loading control.

Excellent, submitted by on
SKU A00282-3
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody EBF1/2/3/4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot results for the target protein EBF2 and the internal control ACTB across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-COX2 Antibody (A00084-2) generated distinct bands and marked expression differences across groups in WB using mouse brown adipose tissue, with GAPDH as loading control.

Excellent, submitted by on
SKU A00084-2
Application Western Blot
Sample Mouse brown adipose tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot (WB) results for the target protein AGT and the internal control ACTB across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-AGT Antibody (A02103-1) yielded well-defined bands and evident expression differences among experimental groups in WB of mouse adipocytes, with ACTB as loading control.

Excellent, submitted by on
SKU A02103-1
Application Western Blot
Sample Mouse adipocytes
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody Angiotensinogen/AGT Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:2000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ABBKINE
Results Summary The image displays Western blot (WB) results for the target protein AGT and the internal control ACTB across different experimental groups. The target bands are clear and distinct, showing significant differential expression between the experimental groups; the results are satisfactory.

Anti-STING Antibody (PB9513) delivered clear target bands in WB of mouse macrophages with faint off-target signals, and β-tubulin was applied as loading control.

Excellent, submitted by on
SKU PB9513
Application Western Blot
Sample Mouse macrophages
Sample Processing Description Cells were lysed in RIPA lysis buffer supplemented with PMSF (100:1) for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected and mixed with 5× Loading Buffer. Samples were denatured at 100°C for 10 min and then loaded onto an SDS-PAGE gel.
Other Reagents Blocking buffer: 5% non-fat milk
Primary Antibody TMEM173 Antibody Picoband®
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:2000, incubated for 1 hour at RT.<
Detection ECL, Imaging system: UNITEC CAMBRIDGE
Results Summary The figure displays Western blot results for the target protein STING and the internal control β-tubulin in various types of macrophages. The target bands are clear and distinct, and the experimental results are satisfactory.

Anti-pIRF3 Antibody (BM4844) produced sharp target bands in WB assays on mouse macrophages with faint non-specific bands, and β-tubulin served as the loading control.

Excellent, submitted by on
SKU P00165
Application Western Blot
Sample Mouse macrophages
Sample Processing Description Cells were lysed in RIPA lysis buffer supplemented with PMSF (100:1) for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected and mixed with 5× Loading Buffer. Samples were denatured at 100°C for 10 min and then loaded onto an SDS-PAGE gel.
Other Reagents Blocking buffer: 5% non-fat milk
Primary Antibody Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
Primary Incubation 1:2,000 dilution, incubated overnight at 4°C.
Secondary Antibody HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation 1:2000, incubated for 1 hour at RT.<
Detection ECL, Imaging system: UNITEC CAMBRIDGE
Results Summary The figure displays Western blot results for the target protein pIRF3 and the internal control β-tubulin in various types of macrophages. The target bands are clear and distinct, and the experimental results are satisfactory.
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