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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
Breast Cancer Regulation
--Milos Lazic, JECURE THERAPEUTICS, JECURE THERAPEUTICS, SCIENTIST II
"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."
--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE
"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."
--Tho Pham, UCONN, NUTRITIONAL SCIENCES, GA
"This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon."
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)
"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."
--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT
"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."
--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST
"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."
--J L, UIC, DENTISTRY, SCIENTIST
"This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased at the proliferation/remolding stage. Easy to use and less expensive. Highly recommended."
--Lu Yang, UCSD, CMMW, GRADUATE STUDENT
"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells...The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. Good ELISA kit for human TFF1 protein. "
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."
--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS
"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."
--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC
"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."
--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN
"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."
--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE
"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."
--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT
"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."
--Aijun Qiao, CANCER CENTER, AUGUSTA UNIVERSITY, POSTDOCTORAL FELLOWS
"We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis. Although there are some non-specific bands, we still can get the strong and clear bands that we want to. The WB band is strong even when you use high dilution concentration. Overall this antibody is good to check UCP1 levels using WB technology."
--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT
"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."
--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."
--Michael Conlin, GENETICS AND MOLECULAR BIOLOGY, UNC CHAPEL HILL, GRADUATE STUDENT
"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."
--Elena Deryugina, THE CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane...produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20oC. The bottom line: The antibody PA1021-2 is excellent for detecting human CD44 containing C-terminal domain."
--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT
"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."
--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."
--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER
"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."
--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT
"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."
--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST
"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."
--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT
"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."
--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
--RAHUL KUMAR, PHARMACEUTICAL SCIENCES, UNIVERSITY OF COLORADO DENVER, POST DOC
"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."
--Okeanos Research Laboratory, Department of Biological Sciences, Clemson University
"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8µm paraffin sections of oyster’s visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control."
--Antibodyresource validation lab
"Under these experimental conditions... [Boster anti-CD14] demonstrated positive staining of Jurkat cells whilst for all the other tested antibodies negative staining was observed."
"Under these experimental conditions... CD34 P35 [Boster anti-CD14] demonstrated positive staining of Jurkat cells whilst for all the other tested antibodies negative staining was observed."
"Under these experimental conditions...[TNF-α P28 Boster anti-CD14] demonstrated positive staining of the THP-1 cells."
--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE
"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."
--Irina Molina UNIVERSITY OF LOUISVILLE MICROBIOLOGY & IMMUNOLOGY GRADUATE STUDENT b
"I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend."
--Ahmed Hosni ZAGAZIG UNIVERSITY HOSPITAL CLINICAL PATHOLOGY LECTURER b
"I will use this product in my research in order to obtain the master degree. My research title is "The association between GDF 15 level and cardiovascular risk in patients with type 2 diabetes mellitus". I will collect samples from diabetic patients and measure the level of GDF 15 in these samples."
--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow
"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")
--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist
"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")
--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
--Akshay Jain, University of Missouri, Kansas City, Pharmaceutical Sciences, Research Assistant
"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")
Source: Customer feedback submission
--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor
"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."
Source: Customer Feedback Submission
--Peter J. Thompson, UCSF Diabetes Center, Postdoctoral Scholar
"CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks."
--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
--Hua Jiang, Pediatrics, University of Colorado Denver, Research Associate
"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."
--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate
"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."
--Swati Naphade, Aging, Buck Institute, Postdoctoral Fellow
"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."
--Saikat Chakraborty, Psychiatry / Neuroscience,
Northwestern University Feinberg School of Medicine, Research Scientist
"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."
"We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 antibody worked perfectly in detection in an Alzheimer's tissue sample. No extra bands. I recommend."
--Preethi Vijayaraj, UCLA, Pediatrics, Postdoctoral Fellow
"Our research focuses on in vitro modeling of organ fibrosis and drug discovery. The protocol for this product was easy to perform. However, there was some amount of variability with the replicates. Was effective in detecting A2M levels at pg levels. Reasonable sensitivity and reproducibility. Easy to use." (Customer’s Tip: "Follow protocol to the T.")
--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")
--Xiaobing Cui, University of Georgia, Physiology & Pharmacology, Research Scientist
"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")
"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")
"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")
--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student
"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")
"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")
--Mostafa Gabr, Duke University Hospital, Neurosurgery, Postdoctoral Researcher
"I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously. Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord. Excellent, reliable product and easy to use."
--P DPN, Johns Hopkins University, Pathology, Postdoctoral Researcher
"We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it."