Customer Testimonials

Customer Survey

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"Very happy with this product"

Excellent, submitted by on

Source: Customer Feedback Submission

"Very happy with this product"

--Ailin Lepletier de Oliveira

Application IHC
Sample Mouse spleen and lung

"Very happy with this product. We were using an IL-17A from Abcam but found very high endogenous non-specific staining. This product was a much better stain in comparison. We used this antibody (1:200) on the Leica BOND Rxm autostainer, blocked with Sniper/1% BSA and it worked beautifully."

"Works well for immunofluorescence"

Excellent, submitted by on

Source: Customer Feedback Submission

"Works well for immunofluorescence"

--Jason Tennessen

Application IF
Sample Drosophila larval fat body

"We’ve verified the specificity of this custom antibody that Boster generated for us. The antibody "Anti-Fruit Fly Gpdh1 Antibody (DZ41223)” works well for immunofluorescence. A 1:100 dilution of this antibody stains fat body tissues of wild-type larvae but not Gpdh1 mutant. I’m happy to advertise this antibody to the fly community."

Immunofluorescence for Anti-CG6353 Antibody

--Amin Ghabrial  Pathology and Cell Biology  Columbia University  Principal Investigator

Excellent, submitted by on
             
                No Image Available              

"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."

                             
Application Immunofluorescence
Sample 5% BSA as a blocking agent for 30 min at 37°C
Sample Drosophila larvae
Primary Incubation  1:100 at 4 degrees overnight
Blocking Agent 4% horse serum
Secondary Incubation   1:1000 anti-Rb-A488
Tertiary Incubation  N/A
Detection IF
Results Summary Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear.
                             
                         
       

Aqueous mounting media for IHC

--Nikhil Kumar

Excellent, submitted by on
No Image Available

Source: Customer Feedback Submission

Application IHC
Sample Uveal melanoma

"I am using Aqueous mounting media for IHC in Uveal Melanoma sections. The substrate is AEC for peroxidase enzyme, which produces a red end product that is soluble in alcohol."

Anti-Smoothened/SMO Antibody for Flow Cytometry

--Yair Fisher, GliXogen Therapeutics, CSO

Excellent, submitted by on

Source: Customer Feedback Submission

Application Flow Cytometry
Sample C3H10T1/2 mouse cell line

"Overlay histogram showing C3H10T1/2 cells stained with A00451-1 (red line). The cells were blocked with 5% normal goat serum, then incubated with rabbit anti-Smoothened/SMO Antibody (A00451-1, 1:50, 2 μg/0.5x10^6 cells) for 30 min at RT. Alexa Fluor® 647 AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L) (1:250) was used as a secondary antibody for 30 minutes at RT. Isotype control antibody (blue line) used under the same conditions. Unlabelled sample (yellow line) was also used as a control."

Very simple to use with promising results!

--Lakshmi, Biotech

Excellent, submitted by on

Source: Google.com

Applications Immunohistochemistry
Sample Kidney
Primary antibody dilution F4/80 Primary antibody / 1:500

"We have been struggling to get a good signal for macrophages in diabetic kidney. However, this kit helped us get a very good signal in the diabetic kidney compared to the controls. Will use this kit for all the chromogenic stainings planned for my study. Very simple to use with promising results!"

Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody

--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine

Excellent, submitted by on
Application Western blot
Sample Drosophila melanogaster (head and thorax)
Detection Immobilon Forte Western HRP substrate

"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."

Good Zebrafish Mef2 Antibody from Boster Bio

--Jingli Cao, Assistant Professor, Weill Cornell Medical College

Excellent, submitted by on
Application Immunofluorescence
Sample Zebrafish heart
Detection Confocal Microscopy

"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."

Immunohistochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by on
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Lung
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Good Mouse IL-12 ELISA Kit

--Daisy Dai University of Miami Surgery Lab Director / Manager

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Starting Material Crispr MEK and STAT3 KO CAFs
Tips This kit doesn't provide PC

"We investigate how alterations in RAS and STAT3 signaling pathways affect the tumor stroma and immune microenvironment in pancreatic cancer. This kit was used to detect the IL-12 level in CM from Crispr MEK and STAT3 KO CAFs. The whole process was about 7 hours including sample preparation."

Easy to Use Human Laminin ELISA Kit

--John Ong, University of Cambridge Engineering Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application Testing laminin concentration in hydrogel
Starting Material Hydrogel
Tips Supernatant used for ELISA

"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."

Immunohistochemistry for Anti-TPR Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse Brain
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37 °C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry review for CD147 Emmprin BSG antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Prostate cancer
Fixative Fixed with 4% paraformaldehyde
Primary Incubation 4°C overnight
Primary Incubation diluent 5% BSA in TBS
Primary Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Dilution The kit was ready to use, no dilution needed
Secondary Incubation at 37°C for 30 min

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by on

The antibody worked perfectly, Great sensitivity

Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
SampleMouse Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent, submitted by on
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Oesophagus
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry for Anti-CD5 Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-HnRNP D/AUF1/HNRNPD Antibody

Excellent, submitted by on
Application Immunocytochemistry
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Colorectal tissue
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry Review For Anti-CD147 Emmprin BSG Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin embedded)
Blocking step 5% BSA as blocking agent for 30 min at 37°C
Sample Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Great PKC antibody for IHC

Excellent, submitted by on
We used Anti-PKC Alpha Picoband™ Antibody for IHC experiment from mouse intestine tissues. Clear positive staining with the right subcellular locations. Super easy to determine the dilution ratio! Great quality.

Immunohistochemistry for Anti-Mannose Receptor/MRC1

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Mouse skin
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

A Good Drosophila Me31B Antibody for WB

--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Insect, D. melanogaster
Detection ECL

"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."

Verification of Antibody on Recombinant Protein

--Hanjo Hellmann, Washington State University, School of Biological Sciences, Associate Professor

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Recombinant protein expressed in and purified from E. coli
Detection Chemiluminescence

"We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments."

A Good PKC Alpha Antibody for IP and WB

--Ramaz Geguchadze, University of New England, Biomedical Sciences, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Dorsal root ganglia (neuronal cells)
Detection CyDye

"We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab."

Best Calbindin Antibody for IHC in Mouse Cerebellum

--Dinesh Joshi, University of Wisconsin Madison, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application Immunohistochemistry
Sample Mice Cerebellum
Detection Fluorescence

"I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P."

No Complaints. A Simple, Effective ELISA from BosterBio

--David Nelson, University of Wisconsin, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Mouse plasma (with EDTA as anticoagulant)
Detection --

"Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays. 10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad. Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% - but consistent with the product literature. I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37°C) because I didn't want to deal with going back and forth into the room that houses our 37°C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image, Columns A and B are the standard curve." (Customer’s Tip: "100-fold dilution of mouse plasma resulted in all data falling on standard curve.")

Very Efficient ELISA Kit from BosterBio

--Abdullah Mamun, University of Texas Health Science Center at Houston, Neurology, Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Serum
Detection --

"Good sensitivity. I have found detectable levels of CD200. Overall, it is a very good kit for CD200 detection." (Customer’s Tip: "I have used neat samples.")

Good Zebrafish CDHR1a Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

Application Immunofluorescence
Sample Zebrafish embryos, 5 days old
Detection Confocal Microscopy

"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"

Good Zebrafish-Specific Siah1 Antibody from Boster Bio

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

Application Immunofluorescence
Sample Zebrafish embryos 5dpf
Detection Confocal Microscopy

"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"

Good Zebrafish Antibody for Nlz2

--Jakub Famulski, University of Kentucky, Biology, Principal Investigator

Excellent, submitted by on

Source: Biocompare.com

Application Immunofluorescence
Sample Zebrafish embryos 24hpf
Detection Confocal Microscopy

"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."

Great TNF-Alpha Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")

Great IL-6 Kit from Boster!

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimize dilutions 1:5-1:250.")

Immunohistochemistry for Anti-VE-Cadherin-5 CDH5-Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Liver
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C Overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunocytochemistry for Anti-CD147/BSG Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Rectum
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunocytochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Rat Brain Tissue
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunohistochemistry for Anti-P53/TP53 Antibody

Excellent, submitted by on
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Ovary
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

Immunocytochemistry forAnti-P53/TP53 Antibody

Excellent, submitted by on
Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

"Awesome Kip2 Antibody from Boster Bio"

Excellent, submitted by on

Source: Biocompare.com

"Awesome Kip2 Antibody from Boster Bio"

---P DPN, Johns Hopkins University, Pathology, Postdoctoral Researcher

Application Immunohistochemistry
Sample Mice kidney
Detection DAB chromogen

"We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it."

Immunoflorescence Review for Anti-EpCAM Antibody

Excellent, submitted by on
Application Immunofluorescence (IF)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human rectal
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

It is a good kit with good quality.

--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Brain Tissue Lysate
Detection --

"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."

Excellent Caspase-3 Antibody from Boster Bio

--Mostafa Gabr, Duke University Hospital, Neurosurgery, Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

Application Immunohistochemistry
Sample Rat spinal cord
Detection Fluorescent microscope

"I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously. Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord. Excellent, reliable product and easy to use."

Quantification of BDNF Post-Transfection in Primary Neuronal Cells

--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Cell lysate and culture medium
Detection --

"The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.")

Adiponectin Analysis in Mouse Serum and Culture Media

--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Serum, tissue homogenate
Detection --

"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")

IL-6 Estimation in Cell Culture Supernatant

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Cell culture media
Detection --

"Good estimation of IL-6. The results were comparable and reproducible each time. Good brand name, very good quality of reagents. Overall justified results with quality of the product. Also good value for the money. Order 4 or more kits to get good discount. That was helpful in my case. Will definitely purchase again throughout my project." (Customer’s Tip: "If performing for 6-well plate supernatant, may run a little higher in concentration. Consider diluting samples on doing a test run.")

Excellent Quantification In Vitro and In Vivo

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Cell culture media, mouse serum
Detection --

"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")

Good reproducible estimation

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Centrifuged cell culture media supernatant
Detection --

"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")

Pretty good product with a reasonable price

--Xiaobing Cui, University of Georgia, Physiology & Pharmacology, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Serum and supernatant
Detection --

"I did this assay twice. Both times were very good. First time I diluted the mouse serum and cell culture supernatant for 100-fold. However, the values of the serum are out of the range of standards. Then, I repeated the assay by diluting the mouse serum for 3000-fold. Much better than the 1st time, but still some values are marginally out of the range of standards. It is recommended that to do a dilution test using small amount of samples. Generally, you can get a very good result, just follow the instruction of this product. Easy to use. Good quality of data. It is a pretty good product with a reasonable price." (Customer’s Tip: "The serum needs to be diluted 5000-fold. The supernatant needs to be diluted 100-fold. Incubate for 10-15 min is enough after adding Color Developing Reagent.")

Easy-to-Use Cytokine Quantification

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Cell culture media
Detection --

"The ELISA kit from Boster Bio was easy to use and gave reproducible results each time. The protocol was well explained and each thing was paid good attention. They also provided tools for data analysis (myassay.com) which I really found useful. The standard plot was as suggested. The cytokines analyzed were comparable to literature reported. Will definitely purchase again. Excellent product." (Customer’s Tip: "Stick to the protocol.")

Immunohistochemistry for Anti-Cyclophilin B/PPIB Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry of Anti-Histone H2A.X/H2AFX Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded )
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human colon
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37 °C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 2ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Reasonable sensitivity and reproducibility

--Preethi Vijayaraj, UCLA, Pediatrics, Postdoctoral Fellow

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample Cell culture supernatant
Detection --

"Our research focuses on in vitro modeling of organ fibrosis and drug discovery. The protocol for this product was easy to perform. However, there was some amount of variability with the replicates. Was effective in detecting A2M levels at pg levels. Reasonable sensitivity and reproducibility. Easy to use." (Customer’s Tip: "Follow protocol to the T.")

Anti-TRPC6 Tried in Human Tissue Sample

--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Human Tissue
Detection Fluorescent based

"We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 antibody worked perfectly in detection in an Alzheimer's tissue sample. No extra bands. I recommend."

Anti-TRPC5 Tried in Human Tissue Sample

--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Human Tissue
Detection Fluorescent based

"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."

Good TIMP-1 Antibody for Western Blotting

--Swati Naphade, Aging, Buck Institute, Postdoctoral Fellow

Excellent, submitted by on

Source: Biocompare.com

Application Western Blot
Sample Human NSC cell lysates
Detection ECL chemiluminescence

"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."

Immunocytochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by on
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Bowel
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 min
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Immunohistochemistry for Anti-CD41/Integrin Alpha 2b/ITGA2B Antibody

Excellent, submitted by on

This antibody works perfectly well!

Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Prostate
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4 °C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1 ug/ml
Secondary Antibody Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
Secondary Ab Dilution Use at an assay dependent concentration
Secondary Ab Incubation at 37°C for 30 min

User Friendly And Economical ELISA Kit

--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate

Excellent, submitted by on

Source: Biocompare.com

Application ELISA
Sample "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells
Detection --

"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."

ADO Antibody Works in Western Blot

--Hua Jiang, Pediatrics, University of Colorado Denver, Research Associate

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Human liver, rat liver, mouse liver, mouse kidney, and mouse brain
Detection Typhoon 940

"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."

It works far better compared to other vendors

--Saikat Chakraborty, PhD, Feinberg School of Medicine, Northwestern University

Excellent, submitted by on

Source: Customer Feedback Submission

Applications Western Blot
Sample Mouse brain lysate
Detection Licor Imaging and ImageJ

"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."

This antibody gives strong membrane staining

--Peter J. Thompson, UCSF Diabetes Center, Postdoctoral Scholar

Excellent, submitted by on

Source: Customer Feedback Submission

Applications IHC-Paraffin, IHC-cryo
Sample NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded
Detection Zeiss Apotome widefield fluorescence microscope

"CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks."

PB9920 successfully extracted rat osteocalcin from rat EDTA plasma

--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor

Excellent, submitted by on

Source: Customer Feedback Submission

Applications Picomole scale immunoprecipitation
Sample Rat EDTA plasma
Detection Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF)

"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."

Very Easy To Work With And Great Results With Least Standard Error

--Akshay Jain, University of Missouri, Kansas City, Pharmaceutical Sciences, Research Assistant

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA (Inflammatory cytokine assay from plasma)
Sample Rat plasma
Detection --

"I used this assay kit for detection of the cytokines in the plasma samples. Only 100 uL of sample yielded absolutely flawless results. Plasma samples showed variable cytokine expression among independent groups which demonstrates the accuracy of this kit. Straightforward protocol. Very easy to perform. Highly recommended product for precise cytokine assay analysis." (Customer’s Tip: "Keep working area clean. Prepare necessary dilution of the sample before starting the procedure.")

Boster's Anti-MDM2 Antibody Worked!

--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample MDA-MB-468 and HEK293E cell lysates
Detection SuperSignal West Pico Plus with a BioRad imager

"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."

Human ADAMTS1 PicoKine ™ ELISA Kit Worked Well

--Huiyuan Tang, University of Utah, Hematology and Hematologic Malignancies, Lab Specialist

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Cell lysates from HUVEC treated with control siRNA or ADAMTS-13 siRNA
Detection --

"I used the kit to test the ADAMTS1 protein level in human endothelial cell lysates. The instructions are clear and the kit worked the first time with reproducible results. Decent expression level was detected and results are reproducible. Cheaper than most comparable product. I will keep using this product and will be happy to recommend to my colleagues." (Customer’s Tip: "Just follow the protocol.")

Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration

--Mahesh Raundhal, Weill Cornell Medicine, Medicine, Postdoctoral Fellow

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Human ovarian cancer ascites
Detection --

"Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1. Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized." (Customer’s Tip: "If using for the first time, plate samples at least 3-4 dilutions to get consistent results among samples at the same dilution.")

The signal was very strong and clean!

--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Whole cell extract of pancreatic tumor cell MIA PaACa2
Detection Enhanced chemiluminescence

"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."

BosterBio Sparcl1 Works

--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

Applications Immunohistochemistry
Sample Mouse astrocytes
Detection Confocal/Fluorescent Scope

"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."

Good quality budget option

--Milos Lazic, JECURE THERAPEUTICS, SCIENTIST II

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample protein concentrations in cell culture media
Detection --

"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."

Kit worked great

--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Primary keratinocytes
Detection --

"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."

Good enough for publishing

--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

We got bands as expected...

--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Mouse lung lysates
Detection SuperSignal West Femto Maximum Sensitivity Substrate

"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."

Great kit

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)

Excellent, submitted by on

Source: Biocompare.com

Applications IL-1RA detection in rat serum
Sample Rat serum
Detection --

"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."

Specific and low variability between lots

--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Purified mouse prolactin and plasma
Detection TMB Substrate

"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."

Good Antibody for Western Blotting

--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample MDCK cell lysate
Detection ECL

"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."

Anti GRK5 (PB 9708) is useful, good.

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."

Outstanding signal of cell bodies and processes

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")

Excellent, submitted by on

Source: Biocompare.com

Applications Immunohistochemistry
Sample Wistar rat brain
Detection Fluorescence

"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."

Reliable and robust

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Excellent, submitted by on
Rat il 6 elisa kit

Source: Biocompare.com

Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Working Antibody for Versican

--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Human cell lines
Detection ECL

"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."

High specificity and stability

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Tumor cell lysate and recombinant protein
Detection SuperSignal West Pico Chemiluminescent Substrate

"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."

I would highly recommend this Boster antibody...

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane
Detection SuperSignal West Pico Chemiluminescent Substrate

"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."

This Antibody Works Perfectly!

--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC

Excellent, submitted by on

Source: Biocompare.com

Applications Immunohistochemistry
Sample Spinal cord
Detection Confocal microscope

"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."

Competitively priced and worked

--Lu Yang, UCSD, CMMW, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

Applications Protein level screen
Sample Cell culture media from MCF7 cells
Detection --

"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells, a human mammary carcinoma which is well known for overexpression of the TFF1 protein. The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. The kit is saturated about 1mg/mL, so in my case, a 1 to 100 dilution of cell culture media was needed in order to be in the linear range of the assay. Good ELISA kit for human TFF1 protein. Worked as advertised."

Good Mouse Rad51 Antibody

--Michael Conlin, GENETICS AND MOLECULAR BIOLOGY, UNC CHAPEL HILL, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample MEFs
Detection LICOR Odyssey

"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."

Good UCP1 Antibody

--Aijun Qiao, CANCER CENTER, AUGUSTA UNIVERSITY, POSTDOCTORAL FELLOWS

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Subcutaneous fat and brown fat tissue
Detection Western Blot

"We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis. Although there are some non-specific bands, we still can get the strong and clear bands that we want to. The WB band is strong even when you use high dilution concentration. Overall this antibody is good to check UCP1 levels using WB technology."

Easy to Use GDNF ELISA Kit

--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

Applications Detecting GDNF
Sample Brain Tissue (Hippocampus)
Detection --

"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."

Works everytime

--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Jurkat Lysates
Detection ECL solution

"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."

The Best DISC1 Antibody on the Market

--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

Applications Western Blot
Sample Jurkat cell lysate
Detection Chemiluminescence

"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."

The standards and ELISA kit worked great.

--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE

Excellent, submitted by on

Source: Biocompare.com

Applications Detection of secreted IL-2 in media of Jurkat T cells
Sample Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep)
Detection --

"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."

Highly recommended

--J L, UIC, DENTISTRY, SCIENTIST

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Skin tissue homogenates
Detection --

"This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased at the proliferation/remolding stage. Easy to use and less expensive. Highly recommended."

Great TNF alpha Kit

--Tho Pham, UCONN, NUTRITIONAL SCIENCES, GA

Excellent, submitted by on

Source: Biocompare.com

Applications ELISA
Sample Cell culture supernatant
Detection --

"This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon."