Testimonials

Review No. Product Name and SKU Validated Application
Review 1 Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) ELISA
Review 2 Human IL-2 PicoKine™ ELISA Kit (EK0397) ELISA
Review 3 Mouse TNF Alpha PicoKine™ ELISA Kit (EK0527) ELISA
Review 4 Human IL-1RA/IL1RN PicoKine™ ELISA Kit (EK0782) ELISA
Review 5 Mouse GDNF PicoKine™ ELISA Kit (EK0935) ELISA
Review 6 Human IL-28A PicoKine™ ELISA Kit (EK0969) ELISA
Review 7 Mouse FGF1 PicoKine™ ELISA Kit (EK1172) ELISA
Review 8 Human TFF1 PicoKine™ ELISA Kit (EK1232) ELISA protein level screen
Review 9 Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (MA1099) Immunohistochemistry
Review 10 Anti-HIF-2-Alpha Antibody (PA1129-2) Western Blot
Review 11 Anti-GFAP Antibody (PA1239) Immunohistochemistry
Review 12 Anti-GRP94 Antibody (PA1340) Western Blot
Review 13 Anti-ABCB11 Antibody (PA1393) Immunohistochemistry
Review 14 Anti-SERCA2 ATPase Antibody (PA1720) Western Blot
Review 15 Anti-CD25/IL-2sR alpha Antibody (PA1747) Western Blot
Review 16 Anti-UCP1 Antibody (PA1982) Western Blot
Review 17 Anti-DISC1 Antibody (PA2023) Western Blot
Review 18 Anti-PLK2 Antibody (PA2131) Western Blot
Review 19 Anti-EGFR Picoband™ Antibody (PB9016) Western Blot
Review 20 Anti-BDNF Picoband™ Antibody (PB9075) Western Blot
Review 21 Anti-Rad51 Picoband™ Antibody (PB9323) Western Blot
Review 22 Anti-CD44 Picoband™ Antibody (new Anti-CD44 SKU: PB9333) Western Blot
Review 23 Anti-CD44 Picoband™ Antibody (PB9333) Western Blot
Review 24 Anti-CD44 Picoband™ Antibody (new Anti-CD44 SKU: PB9333) Western Blot
Review 25 Anti-Versican Picoband™ Antibody (PB9453) Western Blot
Review 26 Anti-GRK5 Picoband™ Antibody (PB9708) Western Blot
Review 27 Anti-GRK6 Picoband™ Antibody (PB9709) Western Blot
Review 28 Anti-Prolactin Antibody (RP1021) ELISA
Review 29 Anti-SPARCL1 Antibody (PA2088) Immunohistochemistry
Review 30 Anti-HMOX1 Antibody (PB9212) Western Blot
Review 31 Anti-NOX2 Antibody (PA1667) Western Blot
Review 32 Anti-CD10 Picoband™ Antibody (PB9058) Western Blot
Review 33 Anti-Rabbit IgG Antibody (GA1013) Electron Microscopy
Review 34 Anti-CD14 Antibody (FC00137) Flow Cytometry
Review 35 Anti-CD34 Antibody (PB9053) Flow Cytometry
Review 36 Anti-CD36 Antibody (PB9246) Flow Cytometry

Maria Teresa Dell'Anno

NEUROSCIENCE, YALE UNIVERSITY

Source: Biocompare.com

"This Antibody Works Perfectly!"

--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC

Excellent

SUMMARY: Anti-GFAP Antibody (PA1239)

Applications Immunohistochemistry
Sample Spinal cord
Detection Confocal microscope

"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."

Elena Deryugina

CELL AND MOLECULAR BIOLOGY, SCRIPPS RESEARCH INST

Source: Biocompare.com

"I would highly recommend this Boster antibody..."

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent

SUMMARY: Anti-EGFR Picoband™ Antibody (PB9016)

Applications Western Blot
Sample Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane
Detection SuperSignal West Pico Chemiluminescent Substrate

"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."

Elena Deryugina

CELL AND MOLECULAR BIOLOGY, SCRIPPS RESEARCH INST

Source: Biocompare.com

"High specificity and stability"

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent

SUMMARY: Anti-CD44 Picoband™ Antibody (PB9333)

Applications Western Blot
Sample Tumor cell lysate and recombinant protein
Detection SuperSignal West Pico Chemiluminescent Substrate

"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."

Gang Ye

BIOLOGY, YORK UNIVERSITY

Source: Biocompare.com

"Working Antibody for Versican"

--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER

Good

SUMMARY: Anti-Versican Picoband™ Antibody (PB9453)

Applications Western Blot
Sample Human cell lines
Detection ECL

"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."

Elena Deryugina

CELL AND MOLECULAR BIOLOGY, SCRIPPS RESEARCH INST

Source: Biocompare.com

"High specificity and stability"

--Elena Deryugina, THE CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent

SUMMARY: Anti-CD44 Picoband™ Antibody (new Anti-CD44 SKU: PB9333)

Applications Western Blot
Sample Tumor cell lysate
Detection Chemiluminescence: West Pico from Thermo Scientific

"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane...produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20oC. The bottom line: The antibody PA1021-2 is excellent for detecting human CD44 containing C-terminal domain."

R.P.

BIOLOGY, PENN STATE UNIVERSITY

Source: Biocompare.com

"Reliable and robust"

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Excellent

SUMMARY: Anti-GRP94 Antibody (PA1340)

Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Lu Yang

CMMW, UCSD

Source: Biocompare.com

"Good price. Worked as advertised."

--Lu Yang, UCSD, CMMW, GRADUATE STUDENT

Good

SUMMARY: Human TFF1 PicoKine™ ELISA Kit (EK1232)

Applications Protein level screen
Sample Cell culture media from MCF7 cells
Detection --

"The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells...The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. Good ELISA kit for human TFF1 protein. "

Shubhankar Nath

MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS

Source: Biocompare.com

"The Best DISC1 Antibody on the Market"

--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT

Excellent

SUMMARY: Anti-DISC1 Antibody (PA2023)

Applications Western Blot
Sample Jurkat cell lysate
Detection Chemiluminescence

"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."

Jason Somarelli

MEDICINE, DUKE UNIVERSITY

Source: Biocompare.com

"The standards and ELISA kit worked great."

--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE

Excellent

SUMMARY: Human IL-2 PicoKine™ ELISA Kit (EK0397)

Applications Detection of secreted IL-2 in media of Jurkat T cells
Sample Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep)
Detection --

"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."

Unattributed Review*

Source: Biocompare.com

"Outstanding signal of cell bodies and processes"

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")

Excellent

SUMMARY: Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (MA1099)

Applications Immunohistochemistry
Sample Wistar rat brain
Detection Fluorescence

"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."

Yongzhen Guo

EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY

Source: Biocompare.com

"Anti GRK5 (PB 9708) is useful, good."

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Excellent

SUMMARY: Anti-GRK5 Picoband™ Antibody (PB9708)

Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."

Sona Balasubramaniam

BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL

Source: Biocompare.com

"Good Antibody for Western Blotting"

--Sona Balasubramaniam, BIOMEDICAL RESEARCH, ALFRED I DUPONT HOSPITAL FOR CHILDREN, POSTDOCTORAL RESEARCH ASSOCIATE

Good

SUMMARY: Anti-SERCA2 ATPase Antibody (PA1720)

Applications Western Blot
Sample MDCK cell lysate
Detection ECL

"This antibody worked brilliantly for both human and canine kidney cells. There are clean single bands, not too much background. Easy to use. Good antibody."

Tabitha Doci

ELISA SPECIALIST, MOLECULAR INNOVATIONS

Source: Biocompare.com

"Specific and low variability between lots"

--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST

Excellent

SUMMARY: Anti-Prolactin Antibody (RP1021)

Applications ELISA
Sample Purified mouse prolactin and plasma
Detection TMB Substrate

"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."

Unattributed Review*

Source: Biocompare.com

"Great kit"

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".)

Good

SUMMARY: Human IL-1RA/IL1RN PicoKine™ ELISA Kit (EK0782)

Applications IL-1RA detection in rat serum
Sample Rat serum
Detection --

"I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting an altered formulation of the protein."

Christina Klinger

TRANSLATIONAL& REGENERATIVE MED, UNIV. OF ARIZONA

Source: Biocompare.com

"We got bands as expected..."

--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS

Good

SUMMARY: Anti-HIF-2-Alpha Antibody (PA1129-2)

Applications Western Blot
Sample Mouse lung lysates
Detection SuperSignal West Femto Maximum Sensitivity Substrate

"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."

Michael Conlin

GENETICS & MOLECULAR BIO, UNC CHAPEL HILL

Source: Biocompare.com

"Good Mouse Rad51 Antibody"

--Michael Conlin, GENETICS AND MOLECULAR BIOLOGY, UNC CHAPEL HILL, GRADUATE STUDENT

Good

SUMMARY: Anti-Rad51 Picoband™ Antibody (PB9323)

Applications Western Blot
Sample MEFs
Detection LICOR Odyssey

"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."

Aijun Qiao

CANCER CENTER, AUGUSTA UNIVERSITY

Source: Biocompare.com

"Good UCP1 Antibody"

--Aijun Qiao, CANCER CENTER, AUGUSTA UNIVERSITY, POSTDOCTORAL FELLOWS

Good

SUMMARY: Anti-UCP1 Antibody (PA1982)

Applications Western Blot
Sample Subcutaneous fat and brown fat tissue
Detection Western Blot

"We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis. Although there are some non-specific bands, we still can get the strong and clear bands that we want to. The WB band is strong even when you use high dilution concentration. Overall this antibody is good to check UCP1 levels using WB technology."

Hannah Oakes

BIOMED SCIENCES, E. TENNESSEE STATE UNIV.

Source: Biocompare.com

"Easy to Use GDNF ELISA Kit"

--Hannah Oakes, EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT

Excellent

SUMMARY: Mouse GDNF PicoKine™ ELISA Kit (EK0935)

Applications Detecting GDNF
Sample Brain Tissue (Hippocampus)
Detection --

"We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples from tissue homogenates, but we did and the plates came out great. This product was selected after speaking with a representative about it at a conference. I am extremely glad we decided to go with this kit. This is a great kit. The protocol was easy to follow and optimize. We will be using these kits again."

Prakash A

NEUROSCIENCE, WRIGHT STATE UNIVERSITY

Source: Biocompare.com

"Works everytime."

--Prakash A, NEUROSCIENCE, WRIGHT STATE UNIVERSITY, GRADUATE STUDENT

Excellent

SUMMARY: Anti-CD25/IL-2sR alpha Antibody (PA1747)

Applications Western Blot
Sample Jurkat Lysates
Detection ECL solution

"Our lab is identifying IL-2 receptor components in non-lymphoid primary cell lines. We use Jurkat (E6-1) cell lines as a positive control for our experiments. Got one single band at the correct size. Very sensitive, can use at low concentration. Great antibody. Works well with whole cell lystes from our cell lines."

Chun-Chih Tseng

NORRIS CANCER CENTER, UNIV OF SOUTHERN CALIFORNIA

Source: Biocompare.com

"Good enough for publishing"

--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Average

SUMMARY: Anti-CD44 Picoband™ Antibody (new Anti-CD44 SKU: PB9333)

Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

Tho Pham

NUTRITIONAL SCIENCES, UCONN

Source: Biocompare.com

"Great TNF alpha Kit"

--Tho Pham, UCONN, NUTRITIONAL SCIENCES, GA

Excellent

SUMMARY: Mouse TNF Alpha PicoKine™ ELISA Kit (EK0527)

Applications ELISA
Sample Cell culture supernatant
Detection --

"This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon."

J.L.

DENTISTRY , UIC

Source: Biocompare.com

"Highly recommended"

--J L, UIC, DENTISTRY, SCIENTIST

Excellent

SUMMARY: Mouse FGF1 PicoKine™ ELISA Kit (EK1172)

Applications ELISA
Sample Skin tissue homogenates
Detection --

"This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased at the proliferation/remolding stage. Easy to use and less expensive. Highly recommended."

Hilary Sherman

APPLICATIONS SCIENTIST, CORNING

Source: Biocompare.com

"Anti-ABCB11 Antibody"

--Hilary Sherman, APPLICATIONS, CORNING, APPLICATIONS SCIENTIST

Inconclusive

SUMMARY: Anti-ABCB11 Antibody (PA1393)

Applications Immunohistochemistry
Sample Hepatocyte spheroids
Detection --

"We tried to use the Anti-ABCB11 antibody to stain bile canaliculi staining of sectioned spheroids. We did not have great success in our applications but it was also the fist time we tried this type of staining. We did not get the punctate staining we expected. It came quickly...It did not meet our needs. It may have been the protocol. I sent the stain and the samples to a vendor for processing."

Nancy Tseng

NEUROSURGERY, UC-DENVER

Source: Biocompare.com

"Shows good bands on the positive control"

--Nancy Tseng, NEUROSURGERY, UC-DENVER, SENIOR-PRA

Inconclusive

SUMMARY: Anti-BDNF Picoband™ Antibody (PB9075)

Applications Western Blot
Sample Hek293
Detection ECL

"This anti-BDNF antibody (Boster-PB9075) works great on the positive control every time, but we still have not received results for our samples. Needs troubleshooting...Still waiting on results for our samples."

Yongzhen Guo

EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY

Source: Biocompare.com

"Review of Anti-GRK6 Antibody"

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Poor

SUMMARY: Anti-GRK6 Picoband™ Antibody (PB9709)

Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"Through rat endothelial cells' western blot tests, we can see PB9709 Anti-GRK6 antibody had a high background so that the blot can hardly be seen. The target band can hardly be detected. With the results of the western blots, we don't recommend using it to detect rEC's Grk6 protein."

Wei Zou

MICROBIOLOGY, UNIVERSITY OF KANSAS MEDICAL CENTER

Source: Biocompare.com

"Test of PLK2 in S1 and 293 Cells"

--Wei Zou, MICROBIOLOGY, UNIVERSITY OF KANSAS MEDICAL CENTER, POSTDOC

Poor

SUMMARY: Anti-PLK2 Antibody (PA2131)

Applications Western Blot
Sample Cell lysis sample
Detection ECL method

"I wanted to investigate the expression of PLK2 protein after ShRNA-Plk2 treatment in UT7/Epo-S1 cell. Western blot was applied to test the expression of PLK2 with the antibody from Boster (PA2131, small volume). I chose this antibody because of the small volume and it claimed to work on western blot. A lot of unspecific bands...This antibody may not be good for western blot in UT7/Epo-S1 cell and 293 cells."

Charles Stopford

MICROBIO AND IMMUNOLOGY , UNIV OF N. CAROLINA

Source: Biocompare.com

"Kit worked great"

--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST

Excellent

SUMMARY: Human IL-28A PicoKine™ ELISA Kit (EK0969)

Applications ELISA
Sample Primary keratinocytes
Detection --

"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."

Milos Lazic

SCIENTIST II, JECURE THERAPEUTICS

Source: Biocompare.com

"Good quality budget option"

--Milos Lazic, JECURE THERAPEUTICS, JECURE THERAPEUTICS, SCIENTIST II

Good

SUMMARY: Human IL-1 Beta PicoKine™ ELISA Kit (EK0392)

Applications ELISA
Sample protein concentrations in cell culture media
Detection --

"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."

Ruby C

NEUROSCIENCE, TUFTS UNIVERSITY

Source: Biocompare.com

"BosterBio Sparcl1 Works"

--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT

Average

SUMMARY: Anti-SPARCL1 Antibody (PA2088)

Applications Immunohistochemistry
Sample Mouse astrocytes
Detection Confocal/Fluorescent Scope

"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."

Chi Li

Source: Biocompare.com

"The signal was very strong and clean!"

--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR

Excellent

SUMMARY: Anti-HMOX1 Antibody (PB9212)

Applications Western Blot
Sample Whole cell extract of pancreatic tumor cell MIA PaACa2
Detection Enhanced chemiluminescence

"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."

Rahul Kumar

Source: Biocompare.com

"This NOX2 antibody is highly specific. Highly recommended!"

--RAHUL KUMAR, PHARMACEUTICAL SCIENCES, UNIVERSITY OF COLORADO DENVER, POST DOC

Excellent

SUMMARY: Anti-NOX2 Antibody (PA1667)

Applications Western Blot
Sample 22RV1 cytosolic and membrane fractions
Detection Enhanced chemiluminescence

"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."

Anthoni Goodman

Source: Biocompare.com

"Cheap, prompt shipping"

--Anthoni Goodman CELL, DEVELOPMENTAL, AND INTEGRATIVE BIOLOGY, UNIVERSITY OF ALABAMA AT BIRMINGHAM, GRADUATE STUDENT/RESEARCH TRAINEE

Inconclusive

SUMMARY: Anti-CD10/Neprilysin Antibody (PB9058)

Applications Western Blot
Sample Rat PFC
Detection Protein Simple, Simple Western size-based assay

"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate."

Okeanos Research Laboratory

Clemson University

Source: Biocompare.com

"Distinctive silver aggregates"

--Okeanos Research Laboratory, Department of Biological Sciences, Clemson University

Excellent

SUMMARY: Anti-rabbit IgG, 5nm colloidal gold conjugated

Applications Electron Microscopy
Sample Oyster Visceral Mass
Detection 5nm Colloidal Gold

"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8µm paraffin sections of oyster’s visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control."

Jonathan Baron

antibodyresource.com

http://www.antibodyresource.com

Source: antibodyresource.com

"[Boster anti-CD14] demonstrated positive staining of Jurkat cells"

--Antibodyresource validation lab

Excellent

SUMMARY: Anti-CD14 Antibody (FC00137)

Applications Flow Cytometry
Sample Jurkat Cells
Detection BD FACSCalibur

"Under these experimental conditions... [Boster anti-CD14] demonstrated positive staining of Jurkat cells whilst for all the other tested antibodies negative staining was observed."

Jonathan Baron

antibodyresource.com

http://www.antibodyresource.com

Source: antibodyresource.com

"[Boster anti-CD34] demonstrated positive staining of Jurkat cells"

--Antibodyresource validation lab

Excellent

SUMMARY: Anti-CD34 Antibody (PB9053)

Applications Flow Cytometry
Sample Jurkat Cells
Detection BD FACSCalibur

"Under these experimental conditions... CD34 P35 [Boster anti-CD14] demonstrated positive staining of Jurkat cells whilst for all the other tested antibodies negative staining was observed."

Jonathan Baron

antibodyresource.com

http://www.antibodyresource.com

Source: antibodyresource.com

"[Boster anti-TNF alpha] demonstrated positive staining of Jurkat cells"

--Antibodyresource validation lab

Excellent

SUMMARY: Anti-TNF Alpha Antibody (PB9246)

Applications Flow Cytometry
Sample THP-1
Detection BD FACSCalibur

"Under these experimental conditions...[TNF-α P28 Boster anti-CD14] demonstrated positive staining of the THP-1 cells."

JUNHUI WANG

UNIVERSITY OF TORONTO

Source: Biocompare.com

"It is a good kit with good quality."

--JUNHUI WANG UNIVERSITY OF TORONTO PHYSIOLOGY RESEARCH ASSOCIATE

Excellent

SUMMARY: Mouse BDNF PicoKine™ ELISA Kit (EK0309)

Applications ELISA
Sample Brain Tissue Lysate

"I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality."

Irina Molina

Source: Biocompare.com

"Great product, reasonably priced, gets consistent, real results."

--Irina Molina UNIVERSITY OF LOUISVILLE MICROBIOLOGY & IMMUNOLOGY GRADUATE STUDENT b

Excellent

SUMMARY: Human MMP-9 PicoKine™ ELISA Kit (EK0465)

Applications ELISA
Sample Cell supernatant
Detection --

"I highly recommend the Boster line of ELISA kits because of their cost and their effectiveness. I had one last round of experiments to run for a publication and we regrettably decided to purchase a lower cost kit from another company. I ran the same samples on two different plates from that company and even my controls were all over the place, resulting in a waste of time and money. This is when we purchased the Boster kit, and by only using one plate, I was able to see the real results of my experiment. My negative and positive controls had the necessary jump and my samples followed the expected trend."

Ahmed Hosni

Source: Biocompare.com

"The over all conclusion about this product is that is a good product, provide acceptable results and it has wide range assay."

--Ahmed Hosni ZAGAZIG UNIVERSITY HOSPITAL CLINICAL PATHOLOGY LECTURER b

Good

SUMMARY: Mouse GDF-15 PicoKine™ ELISA Kit (EK1134)

Applications ELISA
Sample Cell supernatant
Detection --

"I will use this product in my research in order to obtain the master degree. My research title is "The association between GDF 15 level and cardiovascular risk in patients with type 2 diabetes mellitus". I will collect samples from diabetic patients and measure the level of GDF 15 in these samples."