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ELISAs can accurately assess soluble proteins in their native state, so they are ideal for samples such as urine or saliva. Check out the ELISA sample preparation guides to learn how to get the best results from your sample type.
The procedure below provides general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article, the following samples are covered:
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Since serum tends to contain cytokines which may produce significant background signals, we suggest the preparation of serum-free or low-serum medium samples. If it is necessary to test serum-containing medium, we also suggest running an uncultured medium blank to obtain the baseline signals which can then be subtracted from the cultured media sample data.
Guidelines on lysis buffer
Note: Hemolysis should be avoided while collecting serum samples. Those samples that have undergone hemolysis may increase non-specifically staining in HRP-conjugated ELISA assay.
This guide will teach you everything you need to become an ELISA expert, including a critical review of principles, all-in-one FAQs, and more.
Learn the concept behind ELISA assay. Get to know how to maximize the sensitivity and precision of the assay, the plate must be carefully coated with high-affinity antibodies – a process that Boster Bio has mastered.
Learn ELISA Principle
Learn stepwise ELISA protocols from reagent preparation to data analysis. Check out their differences to learn how they compare and their advantages and disadvantage.
Learn our ELISA protocol
Check our ELISA troubleshooting tips
Learn how to perform ELISA data analysis. Get to know the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create a standard curve for analysis.
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