Receive a free secondary antibody with your primary antibody purchase. Browse Secondary Antibodies
Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
Breast Cancer Regulation
Protocols, optimization tips,troubleshooting guides, and more for ELISA.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.
The procedure below provides a general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article, the following samples are covered:
Since serum tends to contain cytokines which may produce significant background signals, we suggest the preparation of serum-free or low-serum medium samples. If it is necessary to test serum containing medium, we also suggest running an uncultured medium blank to obtain the baseline signals which can then be subtracted from the cultured media sample data.
Guidelines on lysis buffer
Note: Hemolysis should be avoided while collecting serum samples. Those samples that have undergone hemolysis may increase non-specifically staining in HRP-conjugated ELISA assay.