Recombinant Protein Expression Service

E.Coli Expression System

Despite the development of the eukaryotic expression systems, E. coli remains the most widely used expression system for recombinant proteins. Advantages of E. coli expression are low cost, high expression level, easy to scale up, short turnaround time. One point to keep in mind is that expression of foreign proteins in E. coli may result in the formation of protein aggregates, commonly known as inclusion bodies, as a result of imbalance between protein aggregation and solubilization. In this case, our protein refolding system are set to solubilize and refold the inclusion bodies and thereby obtain soluble, bioactive recombinant proteins.

Boster’s bacteria expression system serves you with these advantages:

• High Efficiency: Collection of more than 10 host strains and various proprietary or non-proprietary vectors guarantees the selection of the most efficient expression system.
• High Productivity: Enhanced fusion tag technology and proprietary AIE technology are available in house to increase protein solubility and production, and to decrease purification cost, even for toxic proteins.
• Advanced Refolding: We have established and optimized over the years a sophisticated protein refolding system composed of 20 refolding buffers to increase the chance of obtaining soluble and bioactive proteins.
• Large-Scale Production: We are able to produce up to 100L of E. coli cell culture for gram scale production.
• Modifications: Metabolic labeling with stable isotopes (13C and 15N) and biotinylation are available.
• Competitive Pricing: 5mg protein at 80% purity from only $500!