Recombinant Protein Expression Service
Table of Contents
Service Packages Pricing
E.coli Expression Service
Insect Expression Service
Mammalian Cell Expression Service
Boster Guarantee
About Boster Biological Technology
Service Packages Pricing
Package 1
$500+
E.Coli Expression System
The advantages of E.coli expression are low cost, high expression level, easy scalability, and short turnaround time.
Package 2
$1500+
Insect Expression System
The advantages of Insect expression are high efficiency, optimal conditions, large-scale production potential, and competitive pricing
Package 3
$2000+
Mammalian Cell Expression System
The advantages of Mammalian expression are high productivity, large-scale product potential, large capacity, and a high success rate.
E.Coli Expression System
Despite the development of the eukaryotic expression systems, E. coli remains the most widely used expression system for recombinant proteins. Advantages of E. coli expression are low cost, high expression level, easy to scale up, short turnaround time. One point to keep in mind is that expression of foreign proteins in E. coli may result in the formation of protein aggregates, commonly known as inclusion bodies, as a result of imbalance between protein aggregation and solubilization. In this case, our protein refolding system are set to solubilize and refold the inclusion bodies and thereby obtain soluble, bioactive recombinant proteins.
Boster’s bacteria expression system serves you with these advantages:
- High Efficiency: Collection of more than 10 host strains and various proprietary or non-proprietary vectors guarantees the selection of the most efficient expression system.
- High Productivity: Enhanced fusion tag technology and proprietary AIE technology are available in house to increase protein solubility and production, and to decrease purification cost, even for toxic proteins.
- Advanced Refolding: We have established and optimized over the years a sophisticated protein refolding system composed of 20 refolding buffers to increase the chance of obtaining soluble and bioactive proteins.
- Large-Scale Production: We are able to produce up to 100L of E. coli cell culture for gram scale production.
- Modifications: Metabolic labeling with stable isotopes (13C and 15N) and biotinylation are available.
- Competitive Pricing: 5mg protein at 80% purity from only $500!
| Gene Cloning and Expression Test | ||
| Strategy Design | Free | 1 Day |
| Codon Optimization | Free | 2-3 weeks |
| Gene Synthesis | $0.40/bp | |
| Subcloning gene of interest into expression vector | $195 | |
| Expression strain construction and expression optimization | $100 | 1 week |
| Pilot Scale Expression and Purificationon | ||
| 2L Bacterial Cell Culture | From $800 | 2-4 weeks |
| 2-5mg of protein (soluble, >80% purity) | ||
| Large Scale Production | ||
| Large-scale fermentation up to 100L | From $1000 | 2-4 weeks |
| Purification to gram-scale protein | ||
| Protein production to kilogram scale | Contact us | |
Insect Expression System
Insect expression system allows proper refolding, post-translational modification, and oligomerization that are identical to those that occur in mammalian cells. Expression of recombinant proteins is under the control of the strong polyhedrin promoter, allowing expression of up to 50% of the total cell protein. The baculoviral genome is large (130 kb) and can thus accommodate large segments of foreign DNA. Therefore, recombinant proteins can be expressed without size limitations.
Boster’s insect expression system serves you with these advantages:
- High Efficiency: Our system relies on the latest technology in order to ensure high-level expression of both intracellular and secreted recombinant proteins.
- Optimum Conditions: Various insect cell lines, harvest at different time point and test different MOI to guarantee the highest yield.
- Large-Scale Production: Our expression capacity reaches up to 30L per batch and 90-100L monthly.
- Competitive Pricing: Purity greater than 80% starting from $1500!
| Gene Cloning and Expression Test | ||
| Strategy Design | Free | 1 Day |
| Codon Optimization | Free | 2-3 weeks |
| Gene Synthesis | $0.46/bp | |
| Subcloning gene of interest into expression vector | $224.25 | |
| Construct of recombinant bacmid | $115 | 1 week |
| Baculovirus titration using plaque assay (titer 10^8-10^9pfu/ml) | $920 | 2 weeks |
| Pilot Scale Expression and Purificationon | ||
| Infection of 1L of insect cells (Sf-9, Mimic Sf-9, High Five) | From $1725 | 3-4 weeks |
| 1mg of protein (soluble, > 80% purity) | ||
| Large Scale Production | ||
| Large-scale fermentation up to 2-10 liters of insect cell | From $690/L | 3-4 weeks |
| Purification to gram-scale protein | ||
| Additional Services | ||
| Protein refolding, Endotoxin removal, Metabolic labeling, Biotinylation, Lyophilization, Process development | ||
Mammalian cell expression system
Mammalian cell expression system has the most complicated post-translational modifications closest to the native ones. Post-translational modifications are required for appropriate biological activity, immunogenicity, and biodistribution. Modifications such as the authentic glycosylation are essentially only provided by mammalian cells. For this reason, therapeutic and bioactive proteins are usually expressed in mammalian system.
Boster’s mammalian expression system serves you with these advantages:
- High Productivity: Our proprietary vectors support high-level expression of proteins in mammalian cells up to 200mg/L.
- Two Expression Options: We offer both transient transfection and stable cell expression
- Large-Scale Production: Our serum-free suspension cell culture (HEK293 and CHO) allows for gram scale protein production within one month.
- Large Capacity: 10 transient expression projects can be conducted in parallel.
- High Success Rate: We have successful expressed more than 500 proteins in our mammalian system with yields above 1mg/L.
| Gene Cloning and Expression Test | ||
| Strategy Design | Free | 1 Day |
| Codon Optimization | Free | 2-3 weeks |
| Gene Synthesis | $0.46/bp | |
| Subcloning gene of interest into expression vector | $224.25 | |
| Detect protein expression 24-72 hours post transfection at 12-hour intervals | $575 | 1 week |
| Selection of high-expression stable cell line (optional) | From $3450 to $5750 | 2-3 weeks |
| Pilot Scale Expression and Purificationon | ||
| 1 L HEK293 or CHO cell culture | From $1750 | 2-3 weeks |
| 1mg of protein (soluble, > 80% purity) | ||
| Large Scale Production | ||
| Large-scale fermentation up to 500L with 60-80g cell pellet/L | From $2,875 | 4-8 weeks |
| Purification to gram-scale protein | ||
| Protein production to kilogram scale | Please contact us | |
| Additional Services | ||
| Protein refolding, Endotoxin removal, Metabolic labeling, Biotinylation, Lyophilization, Process development | ||
Boster Guarantee
Expertise
Team of experts with decades of combined experience dedicated to expressing your protein.
Communication
A dedicated point of contact for you throughout the project. Weekly project updates available.
Data
Our service team keeps tight monitoring and tracking over each step where data are generated.
About Boster Biological Technology
1993
Serving scientists ever since
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