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Boster offers 9,000+ recombinant proteins and the recombinant protein expression service. From small scale feasibility studies to industrial production of recombinant proteins, let our experienced scientists help you with
your expression and purification needs.
Boster offers a comprehensive selection of recombinant proteins for your research. Over 9,000+ recombinant proteins from human, mouse, rat and a variety of species, these proteins are expressed in E. coli as well as eukaryotic systems.
Boster also provides recombinant protein production service. If you are seeking a vendor for expressing your protein of interest, use our recombinant protein expression expertise for fast and affordable feasibility assessment. If you already have the optimal expression conditions figured out, we can help you scale up to industrial production of the recombinant protein. Send your inquiry to [email protected] for a custom quote.
What is recombinant protein
Recombinant protein expression systems
Transfection and vectors
History of recombinant protein applications
Boster popular recombinant proteins list
Core concepts and a brief history of recombinant technology.
A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses.
There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required.
A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins.
Peter Lobban from Stanford University Medical School first proposed the recombinant DNA idea in the early 1970s. The first successful production of recombinant proteins is documented in 1972 and 1973 from Stanford and
UCSF. Several people were awarded the Nobel Prize for contribution to the recombinant technology. The pharmaceutical industry has been utilizing recombinant technology for producing protein drugs since the early 1980s. One of the most
impactful achievements of recombinant protein technology is the production of recombinant protein insulin. Before recombinant protein insulin is made, diabetes treatment largely depended on insulin from animal sources. The recombinant
insulin significantly lowered treatment cost and enabled a consistent and sufficient worldwide supply for diabetes treatment.
|A2M (PROTP01023)||DCN (PROTP07585-1)||HPRT1 (PROTP00492-1)||POMC (PROTP01189-1)|
|ABO (PROTP16442)||DDT (PROTP30046)||HSPA5 (PROTP11021)||PON1 (PROTP27169)|
|ackA (PROTP0A6A3)||DEFB103ADEFB103B (PROTP81534)||IFNA2 (PROTP01563-2)||PPP2R1A (PROTP30153)|
|ADA (PROTP00813)||DERP1 (PROTP08176)||IFNB1 (PROTP01574-1)||PROK (PROTP06873)|
|AFP (PROTP02771)||DNASE1 (PROTP24855)||IFNG (PROTP01579-1)||PRTN3 (PROTP24158)|
|AGT (PROTP01019)||DPP4 (PROTP27487-1)||IGF1 (PROTP01343-1)||PSMA3 (PROTP25788)|
|ALB (PROTP02769)||dps (PROTP43313)||IGFBP6 (PROTP24592-1)||PTMA (PROTP06454)|
|ALBGIG20 (PROTP02768-1)||EEF1A1 (PROTP68104)||IL12RB1 (PROTP42701)||PTX3 (PROTP26022-1)|
|Aldoc (PROTP05063)||EEF2 (PROTP13639)||IL1A (PROTP01583)||RBM3 (PROTP98179)|
|AMH (PROTP03971)||EFNB2 (PROTP52799)||Il1b (PROTP10749-1)||S100a10 (PROTP08207)|
|ampC (PROTP00811)||EGF (PROTP01133)||Il2 (PROTP04351)||S100a8 (PROTP27005)|
|ANXA5 (PROTP08758)||EGFR (PROTP00533-2)||IL3 (PROTP22629)||SELP (PROTP16109)|
|APOC1 (PROTP02654)||ENO1 (PROTP06733)||IL6 (PROTP05231-1)||SERPINE1 (PROTP05121-1)|
|APOE (PROTP02649)||EPO (PROTP01588-1)||INS (PROTP01308)||SHBG (PROTP04278)|
|APP (PROTP05067)||EPOR (PROTP19235)||KRAS (PROTP01116-1)||SLPI (PROTP03973)|
|ASGR2 (PROTP07307)||ERBB2 (PROTP04626-2)||LDHA (PROTP00338)||SNCA (PROTP37840-8)|
|ASS1 (PROTP00966)||F8 (PROTP00451)||LHB (PROTP01232)||SOD1 (PROTP00441)|
|AVD (PROTP02701)||FCER1A (PROTP12319-1)||LIF (PROTP15018-2)||spa (PROTP38507-2)|
|B2M (PROTP61769-3)||FGF1 (PROTP05230)||LMNA (PROTP02545)||SPINK1 (PROTP00995)|
|BDNF (PROTP23560-2)||FGF2 (PROTP09038-1)||LOX (PROTP28300)||SPR (PROTP35270)|
|BMP2 (PROTP12643-1)||Fkbp1a (PROTP26883)||LPL (PROTP06858)||SPRR1B (PROTP22528)|
|BMP7 (PROTP18075)||FSHB (PROTP01228)||LRG1 (PROTP02750)||SSB (PROTP05455)|
|BRAF (PROTP15056)||FST (PROTP19883-2)||lss (PROTP10547)||ST6GAL1 (PROTP15907)|
|C5 (PROTP01031)||FTH1 (PROTP02794)||malE (PROTP0AEX9-1)||TCF4 (PROTP15884-1)|
|C9 (PROTP02748)||FURIN (PROTP09958)||MAOA (PROTP21397)||TF (PROTP02787)|
|CCL18 (PROTP55774-1)||GALT (PROTP07902)||MB (PROTP02144-2)||TFRC (PROTP02786)|
|CCL5 (PROTP13501)||GBA (PROTP04062)||MBL2 (PROTP11226)||Tgfb1 (PROTP04202)|
|CD38 (PROTP28907)||GCG (PROTP01275-2)||MBP (PROTP02686)||TGFB3 (PROTP10600-5)|
|CDA (PROTP32320)||Gdf7 (PROTP43029)||MIF (PROTP14174-3)||TMPRSS15 (PROTP98073)|
|Cdh5 (PROTP55284)||GFP (PROTP42212)||MMP9 (PROTP14780)||TNF (PROTP01375-3)|
|CEACAM5 (PROTP06731)||GH1 (PROTP46407)||MPO (PROTP05164)||TOP1 (PROTP11387)|
|CFB (PROTP00751)||GHR (PROTP10912-1)||MX1 (PROTP20591)||TYRP1 (PROTP17643)|
|CFD (PROTP00746)||GNAI1 (PROTP63096)||NCL (PROTP19338)||USF1 (PROTP22629-1)|
|CKM (PROTP06732)||GNRH1 (PROTP01148-1)||NGF (PROTP01138-2)||VAMP2 (PROTP63027-1)|
|CLU (PROTP10909-1)||GPC3 (PROTP51654)||PDGFB (PROTP01127-3)||VCAM1 (PROTP19320)|
|COL3A1 (PROTP02461)||GRN (PROTP28799)||PDIA3 (PROTP30101)||VEGFA (PROTP15692-6)|
|CRHBP (PROTP24387)||H3F3AH3F3B (PROTP84243)||PGF (PROTP49763)||WNT3A (PROTP56704)|
|CRP (PROTP02741-2)||HAPLN1 (PROTP10915)||phrB (PROTP00914)||YBX1 (PROTP67809)|
|CXCL8 (PROTP10145)||HLA-DRA (PROTP01903)||PKM (PROTP14618)|
|CYP2D6 (PROTP10635)||HMGB1 (PROTP09429-1)||PLAT (PROTP00750)|
|CYP2E1 (PROTP05181)||HMGB2 (PROTP26624-1)||PNMT (PROTP11086)|
Here are some quick facts about bosterbio.com
Boster antibodies and ELISA kits are cited over 23,000+ times in publications around the world. According to the big data research company CiteAb.com, Bosterbio is globally the fastest growing antibody company in terms of the number of academic publication citations. For Picokine® ELISA kits specifically, an estimate of 6000+ publications are available. You can find specific citations for ELISA kits and antibodies on each product page.
What life scientists are saying about Boster ELISA kits
Maria Teresa Dell'Anno
This Antibody Works Perfectly!
I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again.
Immunohistochemistry , Spinal cord , Confocal microscope
Verification Of Antibody On Recombinant Protein
We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.
Western Blot , Recombinant protein expressed in and purified from E. coli , Chemiluminescence
A Good PKC Alpha Antibody For IP And WB
We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab
Western Blot , Dorsal root ganglia (neuronal cells) , CyDye
Best Calbindin Antibody For IHC In Mouse Cerebellum
I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P.
Immunohistochemistry , Mice Cerebellum , Fluorescence
Good Zebrafish Antibody For Nlz2
Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.
Immunohistochemistry , Zebrafish embryos 24hpf , Confocal Microscopy
Awesome Kip2 Antibody From Boster Bio
We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it.
Immunohistochemistry , Mice kidney , DAB chromogen
Quantification Of BDNF Post-Transfection In Primary Neuronal Cells
The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.
ELISA , Cell lysate and culture medium
Adiponectin Analysis in Mouse Serum and Culture Media
I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.
ELISA , Serum, tissue homogenate
Good TIMP-1 Antibody for Western Blotting
We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it.
Western Blot , Human NSC cell lysates , ECL chemiluminescence
Peter J. Thompson
This Antibody Gives Strong Membrane Staining
CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks.
IHC-Paraffin, IHC-cryo , NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded , Zeiss Apotome widefield fluorescence microscope
The Signal Was Very Strong And Clean!
Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.
Western Blot , Whole cell extract of pancreatic tumor cell MIA PaACa2 , Enhanced chemiluminescence