Recombinant proteins

Hanjo Hellmann

Associate Professor

Verification Of Antibody On Recombinant Protein

We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.

Western Blot , Recombinant protein expressed in and purified from E. coli , Chemiluminescence

Ramaz Geguchadze

Research Scientist

A Good PKC Alpha Antibody For IP And WB

We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab

Western Blot , Dorsal root ganglia (neuronal cells) , CyDye

Dinesh Joshi

Research Scientist

Best Calbindin Antibody For IHC In Mouse Cerebellum

I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P.

Immunohistochemistry , Mice Cerebellum , Fluorescence

Jakub Famulski

Principal Investigator

Good Zebrafish Antibody For Nlz2

Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.

Immunohistochemistry , Zebrafish embryos 24hpf , Confocal Microscopy

P DPN

Postdoctoral Researcher

Awesome Kip2 Antibody From Boster Bio

We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it.

Immunohistochemistry , Mice kidney , DAB chromogen

Sanjay Arora

Graduate Student

Quantification Of BDNF Post-Transfection In Primary Neuronal Cells

The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.

ELISA , Cell lysate and culture medium

Sanjay Arora

Graduate Student

Adiponectin Analysis in Mouse Serum and Culture Media

I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.

ELISA , Serum, tissue homogenate

Swati Naphade

Postdoctoral Fellow

Good TIMP-1 Antibody for Western Blotting

We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it.

Western Blot , Human NSC cell lysates , ECL chemiluminescence

Peter J. Thompson

Postdoctoral Scholar

This Antibody Gives Strong Membrane Staining

CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks.

IHC-Paraffin, IHC-cryo , NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded , Zeiss Apotome widefield fluorescence microscope

Chi Li

Professor

The Signal Was Very Strong And Clean!

Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.

Western Blot , Whole cell extract of pancreatic tumor cell MIA PaACa2 , Enhanced chemiluminescence