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For a more comprehensive ELISA optimization guide, download our how to become ELISA expert eBook.Download ELISA ebook
Boster provides ELISA CRO services in a simple process: send us your samples, we test them for your analytes of interest and send you the results data.
There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between reducing background and maintaining specificity. Use this guide to help decide which type of blocking buffer is best suited for your specific application.
Keywords: ELISA, optimization, blocking, buffer, protein blocker, detergent blockerLearn More About Blocking Optimization
One of the defining features of an ELISA assay is the ability to quickly and effectively wash away unbound molecules. This allows ELISA assays to capture specific antigens or antibodies from crude samples, enabling researchers to identify, isolate, and quantify nearly any molecule. Optimizing the parameters of the wash steps is critical to obtaining the best results from your ELISA assay. Use this guide to help decide how you will optimize the parameters of your washes:
Keywords: ELISA, optimization, antibody, antigen, molecules, ELISA assay, sample, concentration, plate washing, wash parametersLearn More About Washing Optimization
In order to get the best results from your ELISA assay, the dilution factors of the sample and the detection antibodies must be optimized. For strong, quantifiable signal, use a checkerboard titration to test for the optimal concentration of sample and detection antibodies.
Keywords: ELISA, optimization, antibody, antigen, sample, concentration, checkerboard, titrationLearn More About Checkerboard Titration
The choice of which detection system to use is influenced by the equipment available and the needs of the specific application being considered. Use this guide to help decide whether to use enzyme-linked secondary antibodies, or fluorescently labelled secondary antibodies as your detection system.
Keywords: ELISA, optimization, enzyme, fluorescent, enzyme, conjugate, secondary antibodiesLearn More About Detection Systems