Buffer Recipes

Boster Bio Common Buffers

Learn about buffer recipes and find pre-made easy to use buffers packets for protein extraction, washing, and many more applications. Our buffers have been cited in over 6,000 publications.

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Citrate Buffer Powder

74 References 1 Q&As

2 images

$3
Cat # AR0024
1 pack

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The Buffers in science

Buffers are essential for several steps in many protocols to generate publishable data. Boster provides a wide range of general purpose buffers, extraction or lysis buffers as well as buffers specific for western blot, immunohistochemistry, ELISA, and other applications. Learn about what buffers can be used for different applications and see our buffer formulations for the most commonly used buffers.

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General purpose buffers

Extraction/Lysis buffers

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Buffer Selection Table

Check out which buffers can be used for different applications

Buffer SKU Application Buffer Notes Buffer Recipe
Citrate Buffer Powder AR0024 IHC
  • IHC antigen retrieval reagent used in HIER to recover antigens masked by cross-linking during fixation
For 2000 mL (pH 6): 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
RIPA Lysis Buffer
  • AR0105-100
  • AR0102-100
WB, ELISA, IF, IP
  • Extraction/lysis buffer
  • Complete cell lysis solution reagent used for rapid and efficient total cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells
  • Effectively extracts cytoplasmic, nuclear, and membrane proteins
For 100mL: 50mM Tris•HCl pH 7.6, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS
SDS-PAGE Protein Loading Buffer 2X (Reducing) AR0131-20 WB
  • Most commonly used sample buffer for SDS-PAGE of denatured proteins in the Laemmli SDS-PAGE system
For 20mL: 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromophenol blue and 0.1 M Tris HCl, pH 6.8
SDS-PAGE Protein Loading Buffer 5X (Reducing)
  • AR1112
  • AR1112-20
WB
  • Most commonly used sample buffer for SDS-PAGE of denatured proteins in the Laemmli SDS-PAGE system
10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, pH 6.8
Antibody Diluent
  • AR1016-2
  • AR1017
WB, IHC, ELISA
  • General purpose buffer
  • Used to prepare primary antibodies (polyclonal & monoclonal)
  • Helps reduce background in IHC staining process
4% Paraformaldehyde (PFA) Solution in PBS AR1068 IHC
  • Ready-to-use solution for sample preparation and fixing cells for IHC
Sample Diluent Buffer AR1106-1 ELISA
  • Used to dilute samples
ABC Diluent Buffer AR1106-3 ELISA
  • For HRP complex dilution
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Buffer Calculator

Easy-to-use tool for you to calculate buffer molarity and prepare buffer solutions

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More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

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Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.