| SKU: | EK0410 |
|---|---|
| Pack Size: | 96wells/kit, with removable strips. |
| Validated Species: | Human |
| Application: | ELISA |
| Sensitivity: | <0.3pg/ml |
| Assay Range: | 4.69pg/ml-300pg/ml |
| Sample Type: | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
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Overview
| Product Name | Human IL-6 PicoKine™ ELISA Kit |
|---|---|
| SKU/Catalog Number | EK0410 |
| Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6. 96wells/kit, with removable strips. |
| Cite This Product | Human IL-6 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: P29-M212 |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Properties
| Sensitivity | <0.3pg/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 4.69pg/ml-300pg/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate). *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine™ ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Capture Antibody | monoclonal antibody from mouse |
| Detection Antibody | polyclonal antibody from goat |
| Normal Levels | Some research articles suggesting the natural levels of IL6 are listed below: |
| Storage | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Kit Components
| Catalog number | Description | Quantity |
| EK0410-CAP | 96-well plate precoated with anti-Human IL6 antibody | 1 |
| EK0410-ST | lyophilized recombinant Human IL6 standard | 10ng/tubex2 |
| EK0410-DA | biotinylated anti-Human IL6 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.
See Prices For Extra ComponentsMaterial Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | IL6 |
|---|---|
| Protein Name | Interleukin-6 |
| Molecular Weight | 21954 MW |
| Uniprot ID | B4DVM1 |
| Alternative Names | cDNA FLJ61423, highly similar to Interleukin-6 ; |
| Research Areas | |immunology|innate immunity|cytokines|interleukins| neuroscience|neurology process|metabolism| cancer|invasion/microenvironment|angiogenesis|angiogenic growth factors|tumor immunology| metabolism|types of disease|obesity|metabolic disorders| |
Background for Interleukin-6
The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Human IL-6 PicoKine™ ELISA Kit Images
Click the images to enlarge.
Human IL-6 PicoKine ELISA Kit standard curve
Intra/Inter Assay Precision
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
| Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
| CV(%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 12 | 11 | 10 | 10 | 0.82 | 8.2% |
| Sample 2 | 38 | 43 | 40 | 42 | 40 | 1.92 | 4.8% |
| Sample 3 | 145 | 149 | 145 | 150 | 147 | 2.27 | 1.5% |
Typical Data Obtained from Human IL-6 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
| Concentration(pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 | ||
| O.D. | 0.039 | 0.107 | 0.186 | 0.327 | 0.616 | 1.062 | 1.923 | 2.420 | ||
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Publications
Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis
Sun Y, Pan J, Zhang N, Wei W, Yu S, Ai L. Sci Rep. 2017 Dec 22;7(1):18079. doi: 10.1038/s41598-017-18056-9. Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
Wen Y, Gan H, Li Z, Sun X, Xiong Y, Xia Y Sci Rep. 2018 Apr 13;8(1):5941. doi: 10.1038/s41598-018-24397-w. Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis
Maggi P, Bruno G, Perilli F, Saracino A, Volpe A, Santoro C, Ladisa N, Angarano G. In Vivo. 2017 Jan 2;31(1):125-131. Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients
Xuanfei L, Hao C, Zhujun Y, Yanming L, Jianping G. Oncotarget. 2017 Mar 28;8(13):21015-21030. doi: 10.18632/oncotarget.15472. Imidazoline I2 receptor inhibitor idazoxan regulates the progression of hepatic fibrosis via Akt-Nrf2-Smad2/3 signaling pathway
Zhou W, Liu X, Zhang X, Tang J, Li Z, Wang Q, Hu R. Oncotarget. 2017 Jul 22;8(35):58903-58917. doi: 10.18632/oncotarget.19440. eCollection 2017 Aug 29. Oroxylin A inhibits colitis by inactivating NLRP3 inflammasome
Qin X, Yan M, Wang X, Xu Q, Wang X, Zhu X, Shi J, Li Z, Zhang J, Chen W. Theranostics. 2018 Jan 1;8(4):921-940. doi: 10.7150/thno.22182. eCollection 2018. Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway.
He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. Pediatric Transplantation, 21(6). doi: 10.1111/petr.12984
Zhou, Y., Jin, X., Jing, Y., Song, Y., He, X., Zheng, L.,…, & Zhang, G. (2017). Porcine parvovirus infection activates inflammatory cytokine production through Toll-like receptor 9 and NF-κB signaling pathways in porcine kidney cells. Veterinary Microbiology, 207, 56-62. doi: 10.1016/j.vetmic.2017.05.030
Csősz, É., Lábiscsák, P., Kalló, G., Márkus, B., Emri, M., Szabó, A.,…, & Márton, I. (2017). Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers. PLoS One, 12(5), e0177282. doi: 10.1371/journal.pone.0177282
Y.-T. ZHUANG1, D.-Y. XU2, G.-Y. WANG3, J.-L. SUN4, Y. HUANG5, S.-Z. WANG
European Review for Medical and Pharmacological Sciences 2017; 21: 302-309
IL-6 induced lncRNA MALAT1 enhances
TNF-α expression in LPS-induced septic
cardiomyocytes via activation of SAA3
Zhijun Wang, Jianhua Xie, Yujiao Yang, Fan Zhang, Shengnan Wang, Ting Wu, Mingyue Shen & Mingyong Xie
Scientific Reports 7, Article number: 40402 (2017)
doi:10.1038/srep40402
Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice
Renke Li Lei Fan Fenglei Ma Yongyan Cao Junwei Gao Huawen Liu Yan Li
Online ISSN:1792-1015
Fen Ji, Zhi-Wei Chen, Wen-Juan Wang, Xia Jiang
Int J Clin Exp Pathol 2017;10(1):661-670
www.ijcep.com /ISSN:1936-2625/IJCEP0040241
Comparison of high flux hemodialysis with hemodialysis
filtration on pro-inflammatory and oxidative stress
related cytokines in uraemic peripheral
blood mononuclear cells
Sun Y, Zhao Y, Yao J, Zhao L, Wu Z, Wang Y, Pan D, Miao H, Guo Q, Lu N. Biochem Pharmacol. 2015 Mar 15;94(2):142-54. Doi: 10.1016/J.Bcp.2015.02.002. Epub 2015 Feb 10. Wogonoside Protects Against Dextran Sulfate Sodium-Induced Experimental Colitis ...
Huang Q, Xue J, Zou R, Cai L, Chen J, Sun L, Dai Z, Yang F, Xu Y. Exp Ther Med. 2014 Nov;8(5):1648-1654. Epub 2014 Sep 11. Nr4A1 Is Associated With Chronic Low-Grade Inflammation In Patients With Type 2 Diabetes.
Sun L, Deng H, He L, Hu X, Huang Q, Xue J, Chen J, Shi X, Xu Y. J Diabetes Complications. 2015 May-Jun;29(4):589-94. Doi: 10.1016/J.Jdiacomp.2014.12.018. Epub 2014 Dec 31. The Relationship Between Nr2E1 And Subclinical Inflammation In Newly Diagno...
Hu Mm, Yang Q, Zhang J, Liu Sm, Zhang Y, Lin H, Huang Zf, Wang Yy, Zhang Xd, Zhong B, Shu Hb. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1509-14. Doi: 10.1073/Pnas.1318227111. Epub 2014 Jan 13. Trim38 Inhibits Tnf??- And Il-1??-Triggered Nf-??b Act...
Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...
Shen Ww, Zhao Jh. Bioelectromagnetics. 2010 Feb;31(2):113-9. Doi: 10.1002/Bem.20535. Pulsed Electromagnetic Fields Stimulation Affects Bmd And Local Factor Production Of Rats With Disuse Osteoporosis.
Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.
Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
Meng H, Shen Y, Shen J, Zhou F, Shen S, Das Un. Lipids Health Dis. 2013 Oct 29;12:160. Doi: 10.1186/1476-511X-12-160. Effect Of N-3 And N-6 Unsaturated Fatty Acids On Prostate Cancer (Pc-3) And Prostate Epithelial (Rwpe-1) Cells In Vitro.
Wang M, Zheng Z, Meng J, Wang H, He M, Zhang F, Liu Y, Hu B, He Z, Hu Q, Wang H. Biomaterials. 2015 Sep;64:78-87. Doi: 10.1016/J.Biomaterials.2015.06.030. Epub 2015 Jun 20. In Vivo Study Of Immunogenicity And Kinetic Characteristics Of A Quantum D...
Chen J, Xu J, Li J, Du L, Chen T, Liu P, Peng S, Wang M, Song H. Int Immunopharmacol. 2015 May;26(1):147-52. Doi: 10.1016/J.Intimp.2015.03.025. Epub 2015 Mar 31. Epigallocatechin-3-Gallate Attenuates Lipopolysaccharide-Induced Mastitis In Rats Via...
Wang Y, Zhang Zz, Wu Y, Zhan J, He Xh, Wang Yl. Exp Ther Med. 2013 Jan;5(1):315-319. Epub 2012 Oct 25. Honokiol Protects Rat Hearts Against Myocardial Ischemia Reperfusion Injury By Reducing Oxidative Stress And Inflammation.
Zhan Y, Wang Z, Yang P, Wang T, Xia L, Zhou M, Wang Y, Wang S, Hua Z, Zhang J. Cell Death Dis. 2014 Jan 9;5:E985. Doi: 10.1038/Cddis.2013.516. Adenosine 5'-Monophosphate Ameliorates D-Galactosamine/Lipopolysaccharide-Induced Liver Injury Through A...
Ma L, Wang G, Chen Z, Li Z, Yao J, Zhao H, Wang S, Ma Z, Chang H, Tian X. Scientificworldjournal. 2014 Mar 16;2014:387640. Doi: 10.1155/2014/387640. Ecollection 2014. Modulating The P66Shc Signaling Pathway With Protocatechuic Acid Protects The In...
Ying Hz, Liu Yh, Yu B, Wang Zy, Zang Jn, Yu Ch. Food Chem Toxicol. 2013 Feb;52:53-60. Doi: 10.1016/J.Fct.2012.10.030. Epub 2012 Nov 2. Dietary Quercetin Ameliorates Nonalcoholic Steatohepatitis Induced By A High-Fat Diet In Gerbils.
Li Q, Zhou X, Kolosov Vp, Perelman Jm. Transl Res. 2010 Dec;156(6):326-34. Doi: 10.1016/J.Trsl.2010.09.001. Epub 2010 Oct 1. Nicotine Suppresses Inflammatory Factors In Hbe16 Airway Epithelial Cells After Exposure To Cigarette Smoke Extract And Li...
Dong Gh, Gong Jp, Li Jz, Luo Yh, Li Zd, Li Pz, He K. Inflammation. 2013 Oct;36(5):1087-93. Doi: 10.1007/S10753-013-9641-Z. Association Between Gene Polymorphisms Of Irak-M And The Susceptibility Of Sepsis.
Li X, Peng F, Xie C, Wu W, Han X, Chen L. Int Immunopharmacol. 2013 Dec;17(4):1125-33. Doi: 10.1016/J.Intimp.2013.10.001. Epub 2013 Oct 14. (E)-3-(3,4-Dimethoxyphenyl)-1-(5-Hydroxy-2,2-Dimethyl-2H-Chromen-6-Yl)Prop-2-En-1-One Ameliorates The Colla...
Guo R, Wu K, Chen J, Mo L, Hua X, Zheng D, Chen P, Chen G, Xu W, Feng J. Cell Physiol Biochem. 2013;32(6):1668-80. Doi: 10.1159/000356602. Epub 2013 Dec 13. Exogenous Hydrogen Sulfide Protects Against Doxorubicin-Induced Inflammation And Cytotoxic...
Wang G, Chen Z, Zhang F, Jing H, Xu W, Ning S, Li Z, Liu K, Yao J, Tian X. Apoptosis. 2014 Sep;19(9):1342-53. Doi: 10.1007/S10495-014-1008-X. Blockade Of Pkc?? Protects Against Remote Organ Injury Induced By Intestinal Ischemia And Reperfusion Via ...
Wei Z, Yang Y, Xie C, Li C, Wang G, Ma L, Xiang M, Sun J, Wei Y, Chen L. Fitoterapia. 2014 Sep;97:172-83. Doi: 10.1016/J.Fitote.2014.06.002. Epub 2014 Jun 9. Synthesis And Biological Evaluation Of Pyranoisoflavone Derivatives As Anti-Inflammatory ...
Xu W, Lu Y, Yao J, Li Z, Chen Z, Wang G, Jing H, Zhang X, Li M, Peng J, Tian X. Shock. 2014 Nov;42(5):440-7. Doi: 10.1097/Shk.0000000000000225. Novel Role Of Resveratrol: Suppression Of High-Mobility Group Protein Box 1 Nucleocytoplasmic Transloca...
Sun Ln, Yang Zy, Lv Ss, Liu Xc, Guan Gj, Liu G. Int Immunopharmacol. 2014 Nov;23(1):236-46. Doi: 10.1016/J.Intimp.2014.08.023. Epub 2014 Sep 4. Curcumin Prevents Diabetic Nephropathy Against Inflammatory Response Via Reversing Caveolin-1 Tyr14 Pho...
Zhang F, Li Zl, Xu Xm, Hu Y, Yao Jh, Xu W, Jing Hr, Wang S, Ning Sl, Tian Xf. Mol Med Rep. 2015 Jan;11(1):269-76. Doi: 10.3892/Mmr.2014.2679. Epub 2014 Oct 16. Protective Effects Of Icariin-Mediated Sirt1/Foxo3 Signaling Pathway On Intestinal Isch...
Ni J, Wang X, Chen S, Liu H, Wang Y, Xu X, Cheng J, Jia J, Zhen X. Brain Behav Immun. 2015 Oct;49:75-85. Doi: 10.1016/J.Bbi.2015.04.014. Epub 2015 Apr 29. Microrna Let-7C-5P Protects Against Cerebral Ischemia Injury Via Mechanisms Involving The In...
Jin Y, Zhang Y, Wan C, Wang H, Hou L, Chang J, Fan K, Xie X. Evid Based Complement Alternat Med. 2013;2013:194976. Doi: 10.1155/2013/194976. Epub 2013 Sep 18. Immunomodulatory Activity And Protective Effects Of Polysaccharide From Eupatorium Adeno...
Lu Dh, Guo Xy, Qin Sy, Luo W, Huang Xl, Chen M, Wang Jx, Ma Sj, Yang Xw, Jiang Hx. World J Gastroenterol. 2015 Feb 7;21(5):1531-45. Doi: 10.3748/Wjg.V21.I5.1531. Interleukin-22 Ameliorates Liver Fibrogenesis By Attenuating Hepatic Stellate Cell Ac...
Xie Wl, Shi Q, Zhang J, Zhang By, Gong Hs, Guo Y, Wang Sb, Xu Y, Wang K, Chen C, Liu Y, Dong Xp. J Mol Neurosci. 2013 Nov;51(3):919-32. Doi: 10.1007/S12031-013-0002-Z. Epub 2013 Mar 24. Abnormal Activation Of Microglia Accompanied With Disrupted C...
Cai C, Chen Y, Zhong S, Ji B, Wang J, Bai X, Shi G. Plos One. 2014 Apr 21;9(4):E95931. Doi: 10.1371/Journal.Pone.0095931. Ecollection 2014. Anti-Inflammatory Activity Of N-Butanol Extract From Ipomoea Stolonifera In Vivo And In Vitro.
Fan Z, Jing H, Yao J, Li Y, Hu X, Shao H, Shen G, Pan J, Luo F, Tian X. Oxid Med Cell Longev. 2014;2014:191624. Doi: 10.1155/2014/191624. Epub 2014 Aug 20. The Protective Effects Of Curcumin On Experimental Acute Liver Lesion Induced By Intestinal...
Zheng Ls, Kaneko N, Sawamoto K. Front Cell Neurosci. 2015 Jan 28;9:5. Doi: 10.3389/Fncel.2015.00005. Ecollection 2015. Minocycline Treatment Ameliorates Interferon-Alpha- Induced Neurogenic Defects And Depression-Like Behaviors In Mice.
Kang X, Li Y, Wei J, Zhang Y, Bian C, Wang K, Wu X, Hu Y, Li J, Yang Y. Plos One. 2013 Nov 1;8(11):E77427. Doi: 10.1371/Journal.Pone.0077427. Ecollection 2013. Elevation Of Matrix Metalloproteinase-9 Level In Cerebrospinal Fluid Of Tick-Borne Ence...
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regulati...
Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...
Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.
Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...
Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...
Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.
Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...
Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.
Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...
Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...
Yang C, Ling H, Zhang M, Yang Z, Wang X, Zeng F, Wang C, Feng J. Mol Cells. 2011 Jun;31(6):531-8. Doi: 10.1007/S10059-011-1025-3. Epub 2011 Apr 20. Oxidative Stress Mediates Chemical Hypoxia-Induced Injury And Inflammation By Activating Nf-??b-Cox-...
Hu Y, Chen X, Duan H, Hu Y, Mu X. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. Doi: 10.3109/08923970902814129. Chinese Herbal Medicinal Ingredients Inhibit Secretion Of Il-6, Il-8, E-Selectin And Txb2 In Lps-Induced Rat Intestinal Microvascula...
Yang J, Yu Hm, Zhou Xd, Kolosov Vp, Perelman Jm. Mol Immunol. 2013 Jan;53(1-2):161-71. Doi: 10.1016/J.Molimm.2012.06.015. Epub 2012 Aug 30. Study On Trpv1-Mediated Mechanism For The Hypersecretion Of Mucus In Respiratory Inflammation.
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Customer Q&As
Q: Does EK0410 work with whole cell lysates?
A: EK0410 is not suitable for working with whole cell lysates.
Question: Does this ELISA kit contain any product produced in humans or primates?
Keywords: component, ingredient
Keywords: component, ingredient
A: None of the components in our ELISA kits are produced in humans or primates.
Q: What is the volume of the recombinant protein control? What is its shelf life?
Keywords: expiration, storage, temperature, size
Keywords: expiration, storage, temperature, size
A: The volume of the control is around 100pg. If unopened, the shelf life of the control is the same as the whole kit - "Store at 4˚C for 6 months, at -20˚C for 12 months." The reconstituted control can be stored at -20˚C for 2 days.
Q: Will it be okay to run the experiment if I accidentally used the wrong buffer (e.g. sample diluent buffer) for antibody dilution instead of the antibody diluent buffer? Keywords: dilution, replace, substitute, sample buffer
A: Using the sample diluent buffer for antibody dilution instead of the antibody diluent buffer will negatively affect the test result to some extent. The values of both standard and sample might be lower than the normal values.
Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead?
Keywords: protocol, alternative buffer, applications, reagent recipe
A: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: Why are my O.D. values different than your values on the datasheet?
Keyword: troubleshooting, reference
A: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly.
Keyword: protocol, applications
A: 0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP?
Keyword: kit components, reagents, protocol
A: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine?
Keyword: applications
A: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Have you tested this kit with pig samples?
Keyword: applications, porcine, detection
A: No, we have not tested this with pig samples. A pilot experiment is recommended first if you plan to detect pig samples.
Q: Can I calculate the results of unknown samples using a computer program with a 4-parameter logistic function?
Keyword: data analysis
A: Yes, 4-parameter logistic function can be used to calculate sample data.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay?
Keyword: protocol, reagents
A: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits?
Keyword: technical support, help, customer service
A: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem?
Keyword: troubleshoot, protocol
A: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits?
Keyword: well capacity, product size
A: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do?
Keyword: wash buffer
A: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals?
Keyword: antibody clonality
A: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers?
Keyword: storage, sequential use
A: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits?
Keyword: preservative
A: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit?
Keyword: positive control, negative control
A: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?
A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?
A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?
A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?
A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?
A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?
A: Yes the plate is separable. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?
A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?
A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?
A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our techincal support.
Q: Can this ELISA kit react with human, mouse, rat or other species?
A: If the kit is reactive to another commonly used species (human, mouse, and/or rat), we would have listed it as a separate product. If you want to check cross-reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to human il 6 elisa kit, human il-6 elisa kit, human il6 elisa kit, human interleukin 6 elisa kit, human interleukin-6 elisa kit
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