Human IL-6/Interleukin-6 ELISA Kit PicoKine™

Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.3pg/ml. Cited in 199 publication(s).

Product Info Summary

SKU: EK0410
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Product Name

Human IL-6/Interleukin-6 ELISA Kit PicoKine™

See all IL-6 products

SKU/Catalog Number

EK0410

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.3pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Sensitivity

<0.3 pg/ml

Assay Range

4.69 pg/ml - 300 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0410 is reactive to IL6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Application Guarantee

EK0410 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0410-CAP Anti-Human IL6 Pre-coated 96-well strip microplate 1
EK0410-ST Human IL6 Standard 2 vials, 10 ng/tube
EK0410-DA Human IL6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04.699.3818.7537.575150300
O.D.0.0310.0910.1270.2220.5130.7491.1861.997

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-6, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-6 in samples: 24401617 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-6/Interleukin-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1038145937130
Standard deviation0.621.6710.730.641.6611.18
CV (%)6.2%4.4%7.4%7.2%4.5%8.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 110121110100.828.2%
Sample 238434042401.924.8%
Sample 31451491451501472.271.5%
*number of samples for each test n=16.

Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05231

Gene ID

3569

Gene Name

IL6

Full Name

Interleukin-6

Weight

23.718kDa

Superfamily

IL-6 superfamily

Alternative Names

B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL6, check out the IL6 Infographic

IL6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0410 has been cited in 199 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function
Species: Human

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Species: Human

Frequency of NFKBIA deletions is low in glioblastomas and skewed in glioblastoma neurospheres
Species: Human

Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy
Species: Human

Klotho suppresses RIG-I-mediated senescence-associated inflammation
Species: Human

Lu F,Chen H,Hong Y,Lin Y,Liu L,Wei N,Wu Q,Liao S,Yang S,He J,Shao Y.A gain-of-function NLRP3 3‘-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression.Sci Rep.2021 Jun 25;11(1):13300.doi:10.1038/s41598-021-92547-8.PMID: 34172780.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE: PLASMA,HUVECS AND THP-1 CELL, DILUTION:NA

Boyang Sun,Siqi Ying,Qian Ma,Han Li,Jie Li,Jinlin Song,Metformin ameliorates HMGB1-mediated oxidative stress through mTOR/AMPK pathway in experimental periodontitis,Genes & Diseases,2021,,ISSN 2352-3042,https://doi.org/10.1016/j.gendis.2021.06.003.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:HPDLCS, DILUTION:NA

Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION: NA

Tutuncu EO,Dundar ZD,Kilinc I,Tutuncu A,Kocak S,Girisgin AS.Prognostic Value of Immunosuppressive Acidic Protein and Oxidative Stress Status in Critically Ill Patients.Indian J Crit Care Med.2021 Apr;25(4):405-409.doi:10.5005/jp-journals-10071-23788.PMID:34045807;PMCID:PMC8138636.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA

Stellavato,A.;Restaino,O.F.;Vassallo,V.;Cassese,E.;Finamore,R.;Ruosi,C.;Schiraldi,C.Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes.Pharmaceutics 2021,13,737.https://doi.org/10.3390/pharmaceutics13050737
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:CHONDROCYTES, DILUTION:NA

Zhang X,Zhu M,Hong Z,Chen C.Co-culturing polarized M2 Thp-1-derived macrophages enhance stemness of lung adenocarcinoma A549 cells.Ann Transl Med.2021 Apr;9(8):709.doi:10.21037/atm-21-1256.PMID:33987407;PMCID:PMC8106048.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:A549 CELL, DILUTION:NA

Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA

Liu T,Yin C,Li Y,Gao F,Yu L,Wang Z,Wang Q.Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study.Clin Interv Aging.2021 May 3;16:757-765.doi:10.2147/CIA.S309082.PMID:33976542;PMCID:PMC8106456.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Erica Costantini,Bruna Sinjari,Katia Falasca,Marcella Reale,Sergio Caputi,Srinivas Jagarlapodii,Giovanna Murmura," Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast",Mediators of Inflammation,vol.2021,Article ID 5562340,9 pages,2021. https://doi.org/10.1155/2021/5562340
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HGF CELL, DILUTION:NA

Pınar Bürhan-Çavuşoğlu,Evin İşcan,Ayşim Güneş,Neşe Atabey,Tunç Alkın,Increased telomerase activity in major depressive disorder with melancholic features: Possible role of pro-inflammatory cytokines and the brain-derived neurotrophic factor,Brain,Behavior,& Immunity - Health,2021,100259,ISSN 2666-3546,https://doi.org/10.1016/j.bbih.2021.100259.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Yang N,Wang H,Zhang R,Niu Z,Zheng S,Zhang Z.C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway.Arch Med Res.2021 May 1:S0188-4409(21)00079-5.doi:10.1016/j.arcmed.2021.03.008.Epub ahead of print.PMID:33947580.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA

Yi Y,Chai X,Zheng L,Zhang Y,Shen J,Hu B,Tao G.CRISPR-edited CART with GM-CSF knockout and auto secretion of IL6 and IL1 blockers in patients with hematologic malignancy.Cell Discov.2021 Apr 27;7(1):27.doi:10.1038/s41421-021-00255-4.PMID:33907185.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Yang M, Wang LI. MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis. J Biosci. 2021;46:28. PMID: 33753579.
Species: Human,Rat
EK0410 usage in article: APP:ELISA, SAMPLE:B/TSMCS, DILUTION:NA

Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021 Mar 12. doi: 10.1007/s10753-021-01445-8. Epub ahead of print. PMID: 33710445.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Li C, Liu M, Liu K, Li M, Liu Y, Li T, Wei Y, Long Y, He W, Shi X, Li Y, Zhang H. BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody. Biochem Biophys Res Commun. 2021 Mar 16;551:155-160. doi: 10.1016/j.bbrc.2021.02.128. Epub ahead of print. PMID: 33740622.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA

Huang C,Chen Y,Lai B,Chen YX,Xu CY,Liu YF.Overexpression of SP1 restores autophagy to alleviate acute renal injury induced by ischemia-reperfusion through the miR-205/PTEN/Akt pathway.J Inflamm (Lond).2021 Feb 5;18(1):7.doi:10.1186/s12950-021-00270-y.PMID
Species: Human,Rat
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA

Junde Hou,Yanxin Cheng,Hongfang Wei et al.Dexmedetomidine Attenuates Hemodynamic and Proinflammatory Responses During Craniotomy for Traumatic Brain Injury:A Randomized Controlled Trial Study,02 February 2021,PREPRINT (Version 1) available at Research Squ
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Qinchao Chen,Meng Cao,Hanyi Ge,"Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis",BioMed Research International,vol.2021,Article ID 8899863,12 pages,2021.https://doi.org/10.1155/2021/8899863
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA

Gao H,Guo Z. LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Med (Wars). 2021 Jan 8;16(1):117-127.doi:10.1515/med-2021-0200.PMID:33542956;PMCID: PMC7819546.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA

Wei W,Yao Y,Bi H,Xu W,Gao Y.Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.Biochem Biophys Res Commun.2021 Jan 8;540:1-7.doi:10.1016/j.bbrc.2020.12.018.Epub ahead of print.PMID:33429194.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA

Shaat, R.M.,Elsaid, T.O.,EL Baz, A. et al. Do obese patients with primary knee osteoarthritis benefit from a single bout of moderate intensity aerobic exercise?.Egypt Rheumatol Rehabil 47,27 (2020).https://doi.org/10. 1186/s43166-020-00029-y
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Fu,C.,Xu,Q.,Tang,S.,Cao,Y.,Liu,C.,Wang,Y.,Qian,Y.,Shi,F.,Gui,J.,Fan,Q., & Ling,Y.(2020).The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury.American journal of translational research, 12(11), 7114–712
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
EK0410 usage in article: APP:ELISA, SAMPLE:MSCS, DILUTION:NA

Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865
Species: Human, Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liao H,Zhang H,Xiang J,Chen G,Cao Z.The effect of the surgical microscope on the outcome of root scaling. Am J Transl Res. 2020 Nov 15;12(11):7199-7210.PMID: 33312360;PMCID:PMC7724328.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov
Species: Human

Xue M, Peng N, Zhu X, Zhang H. Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis. Biochem Biophys Res Commun. 2020 Nov 25:S0006-291X(20)32086-6. doi
Species: Human

Asila A, Wang Y, Miao D, Yang X, Kaisaer Y, Ma L, Lv G, Yang C. SNHG16/miR-485-5p/BMP7 axis modulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Gene Med. 2020 Nov 11:e3296. doi: 10.1002/jgm.3296. Epub ahead of print
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HBMSCS, DILUTION:NA

Yuan X, Zhang L, Huang Y, Liu D, Peng P, Liu S, Long G, Hu G, Sun W. Induction of interleukin-6 by irradiation and its role in epithelial mesenchymal transition and radioresistance of nasopharyngeal carcinoma cells. Head Neck. 2020 Nov 5. doi: 10.1002/hed
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:NPC CELLS, DILUTION:NA

Hu Q, Peng J, Jiang L, Li W, Su Q, Zhang J, Li H, Song M, Cheng B, Xia J, Wu T. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis. 2020 Oct 28;11(10):925. doi: 10.1
Species: Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:CAL 27 AND HSC6 CELL LINES, DILUTION:NA

Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.

Serum Interleukin-6 is Not Linked with Sleep-Quality, Restless Legs Syndrome, and Depression, But with 6-Month Survival in Hematological Malignancies.

Evaluation of second trimester amniotic fluid ADAMTS4, ADAMTS5, interleukin-6 and tumor necrosis factor-α levels in patients with gestational diabetes mellitus.

The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease

Novel therapeutic approach by nicotine in experimental model of multiple sclerosis

Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury

Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis

Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome

From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026

E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury

Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome

Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine

Establishment of a Rabbit Model of Chronic Obstructive Sleep Apnea and Application in Cardiovascular Consequences

Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes

Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis

The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis

Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial

Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis

Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients

Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice

Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression

Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells

Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery

A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes

Placental Growth Factor, Soluble fms-Like Tyrosine Kinase 1, Soluble Endoglin, IL-6, and IL-16 as Biomarkers in Preeclampsia

Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage

Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells

Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial

Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis

LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling

Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease

miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40

NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation.

Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP)

Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes

GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway

Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells

C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells

Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis

Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide

Association of serum immunoglobulin-G to Porphyromonas gingivalis with acute cerebral infarction in the Chinese population

Bclaf1 is an important NF-?B signaling transducer and C/EBP? regulator in DNA damage-induced senescence

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel

miR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes

Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages

Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery

TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells

Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms

RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms

A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-? activity

Evaluation of cytokines as Th1/Th2 markers in pathogenesis of children with Crimean-Congo hemorrhagic fever

Microvesicles secreted from human multiple myeloma cells promote angiogenesis

Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients

X4 viruses are frequently archived in patients with long-term HIV infection but do not seem to influence the ?inflamm-aging? process

Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis

Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy

Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-?B/COX-2 Pathway

Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome

Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...

Sun Y, Pan J, Zhang N, Wei W, Yu S, Ai L. Sci Rep. 2017 Dec 22;7(1):18079. doi: 10.1038/s41598-017-18056-9. Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway

Wen Y, Gan H, Li Z, Sun X, Xiong Y, Xia Y Sci Rep. 2018 Apr 13;8(1):5941. doi: 10.1038/s41598-018-24397-w. Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis

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18 Customer Q&As for Human IL-6/Interleukin-6 ELISA Kit PicoKine™

Question

Is EK0410 still usable if left at room temperature without cooling for 2 days?

Verified customer

Asked: 2020-06-18

Answer

The Human IL-6 ELISA Kit PicoKine™ (EK0410) should be stable but it is not covered by warranty if not stored according to instructions.

Boster Scientific Support

Answered: 2020-06-18

Question

Hello Support: I purchased Human IL-6 ELISA Kit but I have some questions, could you please give advice on staining technique? Is it possible to use only few wells for analysis of several samples and save other plates for further studies? Lastly, do I need to buy any software for data analysis, or Excel will be enough?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The Human IL-6 ELISA Kit comes with 96 well with removable strips, so you can remove as many strips as you need before using it, then store the remaining strips in the pouch with desiccant, sealed it tightly and put in storage at 4C. For your data analysis excel will do. All you need to do is to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve.

Boster Scientific Support

Answered: 2020-04-30

Question

Q: can I use heparin plasma as samples in Human IL-6 Picokine® ELISA Kit (Catalog # EK0410)?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-6 causing disruption of its protein structure. IL-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-6 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-03-20

Question

What is the expiration date of EK0410 from the time of manufacturing, 6 months or 1 year?

Verified customer

Asked: 2019-12-17

Answer

Expiration is based on storage temperature. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.

Boster Scientific Support

Answered: 2019-12-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-22

Question

I purchased Human IL-6 ELISA Kit and I reconstitute with acetic acid and the coating buffer is alkaline solution of pH 9.6. I am concern with the pH change and possibly affects my results? Please let me know

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

We recommend to use for coating buffers, the standard PBS rather than alkaline carbonate buffers for this antibody Human IL-6 ELISA Kit

Boster Scientific Support

Answered: 2019-08-30

Question

Is the Human IL-6 ELISA Kit have been tested with saliva samples? Do you have any suggestions?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The Human IL-6 ELISA Kit is suitable to use in saliva samples and the usual protocol would apply. No need for pre-treatment of sample before assay.

Boster Scientific Support

Answered: 2019-08-15

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-04

Question

What is the normal serum range of EK0410?

Verified customer

Asked: 2019-04-08

Answer

Please see the normal serum range for the Human IL-6 ELISA Kit PicoKine™ (EK0410) below; IL-6 (Catlog no. EK0410), 0-15pg/mL

Boster Scientific Support

Answered: 2019-04-09

Question

Is it normal to have fluid in the well of the EK0410 plate and a solid standard that is very hard to dissolve.

Verified customer

Asked: 2019-04-01

Answer

For the Human IL-6 ELISA Kit PicoKine™ (EK0410), it is normal that there is fluid in the wells of the plate, and the drops could be removed by patting the plate on a paper towel or by aspiration. For the standard, we never encountered this problem before, and our technicians suggests to centrifuge at 2000-4000×g for 5 minutes after dissolving the standard.

Boster Scientific Support

Answered: 2019-04-02

Question

Have you tested EK0410 in tissue homogenates and Cell culture supernates?

Verified customer

Asked: 2019-03-15

Answer

The Human IL-6 EK0410 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-03-18

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-6 using the EK0410 Human IL-6 Picokine® ELISA Kit?

E. Puri

Verified customer

Asked: 2019-03-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-03-11

Question

Please let me know if you CAN supply QC materials for EACH of these kits?. If you could introduce in EACH kit at least 2 or 3 vials with QC material (having 2 or 3 different concentrations of the analyte for which the kits are used) it would be PERFECT! (even if the raw materials of these QC vials will be the same as the standards

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

We can not provide 2 or 3 different concentrations of the analyte(e.g. serum) for those kits. The QC vials we can provide are recombinant proteins of different concentrtaions.

Boster Scientific Support

Answered: 2019-02-12

Question

Does EK0410 work with whole cell lysates?

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

EK0410 is not suitable for working with whole cell lysates.

Boster Scientific Support

Answered: 2018-06-29

Question

What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly. Keyword: protocol, applications

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.

Boster Scientific Support

Answered: 2017-09-13

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Harris

Verified customer

Asked: 2017-07-23

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-07-23

Question

Q: how to proceed with the analysis of ELISA data? I have obtained IL-6 level in plasma.

Verified Customer

Verified customer

Asked: 2016-07-29

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-07-29

Question

We received an inquiry regarding the EK0410 IL-6 ELISA Kit (Human) from a customer:

Verified Customer

Verified customer

Asked: 2016-01-06

Answer

<0.3pg/ml *The sensitivity or the minimum detectable dose (MDD) is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration.

Boster Scientific Support

Answered: 2016-01-06

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