Human IL-6 ELISA Kit PicoKine™

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The Human IL-6 ELISA Kit comes with 96 well with removable strips, so you can remove as many strips as you need before using it, then store the remaining strips in the pouch with desiccant, sealed it tightly and put in storage at 4C. For your data analysis excel will do. All you need to do is to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve.

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We recommend to use for coating buffers, the standard PBS rather than alkaline carbonate buffers for this antibody Human IL-6 ELISA Kit

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The Human IL-6 ELISA Kit is suitable to use in saliva samples and the usual protocol would apply. No need for pre-treatment of sample before assay.

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We can not provide 2 or 3 different concentrations of the analyte(e.g. serum) for those kits. The QC vials we can provide are recombinant proteins of different concentrtaions.

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EK0410 is not suitable for working with whole cell lysates.

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0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.

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<0.3pg/ml *The sensitivity or the minimum detectable dose (MDD) is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration.

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The Human IL-6 ELISA Kit PicoKine™ (EK0410) should be stable but it is not covered by warranty if not stored according to instructions.

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Please see the normal serum range for the Human IL-6 ELISA Kit PicoKine™ (EK0410) below; IL-6 (Catlog no. EK0410), 0-15pg/mL

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For the Human IL-6 ELISA Kit PicoKine™ (EK0410), it is normal that there is fluid in the wells of the plate, and the drops could be removed by patting the plate on a paper towel or by aspiration. For the standard, we never encountered this problem before, and our technicians suggests to centrifuge at 2000-4000×g for 5 minutes after dissolving the standard.

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The Human IL-6 EK0410 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.