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Human IL-6 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6. 96wells/kit, with removable strips. Cited in 170 publication(s).
Human IL-6 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0410 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <0.3pg/ml |
| Assay Range: | 4.69pg/ml-300pg/ml |
Introduction
SOX2 is a transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. SOX4 is important for early embryogenesis and for embryonic stem cell pluripotency.
Overview
| Product Name | Human IL-6 ELISA Kit PicoKine™
See all IL6 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0410 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human IL6 with <0.3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity: |
| Cite This Product | Human IL-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sensitivity | <0.3pg/ml |
| Assay Range | 4.69pg/ml-300pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: P29-M212 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P05231 |
|---|---|
| Gene Name | IL6 |
| Protein Name | Interleukin-6 |
| Subcellular Localization | Nucleus. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human IL-6 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0410-CAP | 96-well plate precoated with anti-Human IL6 antibody | 1 |
| EK0410-ST | lyophilized recombinant Human IL6 standard | 10ng/tube |
| EK0410-DA | biotinylated anti-Human IL6 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0410 Human IL-6 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.039 | 0.107 | 0.186 | 0.327 | 0.616 | 1.062 | 1.923 | 2.420 |
We measured random samples of Human IL-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
| Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
| CV(%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
We measured the performance consistency of Human IL-6 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 12 | 11 | 10 | 10 | 0.82 | 8.2% |
| Sample 2 | 38 | 43 | 40 | 42 | 40 | 1.92 | 4.8% |
| Sample 3 | 145 | 149 | 145 | 150 | 147 | 2.27 | 1.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 170
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| Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome | PubMed ID 2865457 |
| Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-?B/COX-2 Pathway | PubMed ID 21779360 |
| Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy | PubMed ID 22787172 |
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| Microvesicles secreted from human multiple myeloma cells promote angiogenesis | PubMed ID 24374814 |
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| A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-? activity | PubMed ID 25017038 |
| RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms | PubMed ID 25254379 |
| Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms | PubMed ID 25299975 |
| TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells | PubMed ID 25360740 |
| Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery | PubMed ID 25663985 |
| Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages | PubMed ID 25861639 |
| miR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes | PubMed ID 26450708 |
| IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel | PubMed ID 26503059 |
| Bclaf1 is an important NF-?B signaling transducer and C/EBP? regulator in DNA damage-induced senescence | PubMed ID 26794446 |
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| Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide | PubMed ID 26949603 |
| Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis | PubMed ID 26962319 |
| C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells | PubMed ID 27011164 |
| Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells | PubMed ID 27034587 |
| GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway | PubMed ID 27057094 |
| Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes | PubMed ID 27082319 |
| Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP) | PubMed ID 27127347 |
| Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome | PubMed ID 27168826 |
| Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway | PubMed ID 27398311 |
| NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation. | PubMed ID 27403035 |
| miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40 | PubMed ID 27412561 |
| Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease | PubMed ID 27445436 |
| LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling | PubMed ID 27590510 |
| Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis | PubMed ID 27602373 |
| Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial | PubMed ID 27651815 |
| Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells | PubMed ID 27679980 |
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| Placental Growth Factor, Soluble fms-Like Tyrosine Kinase 1, Soluble Endoglin, IL-6, and IL-16 as Biomarkers in Preeclampsia | PubMed ID 27799724 |
| A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes | PubMed ID 28256567 |
| Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery | PubMed ID 28450928 |
| Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells | PubMed ID 28498464 |
| Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression | PubMed ID 28928376 |
| Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice | PubMed ID 28977826 |
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| Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial | PubMed ID 29887900 |
| The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis | PubMed ID 29900992 |
| Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis | PubMed ID 26673286 |
| The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility | PubMed ID 29914245 |
| Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes | PubMed ID 26239742 |
| Small molecule GL-V9 protects against colitis-associated colorectal cancer by limiting NLRP3 inflammasome through autophagy | PubMed ID 29296531 |
| Establishment of a Rabbit Model of Chronic Obstructive Sleep Apnea and Application in Cardiovascular Consequences | PubMed ID 28218220 |
| Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine | PubMed ID 29049953 |
| Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome | PubMed ID 20346168 |
| Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026 | PubMed ID 28776753 |
| E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury | PubMed ID 28740545 |
| From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026 | PubMed ID 29036520 |
| Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome | PubMed ID 29062341 |
| Protection of SAL B with H9C2 cells | PubMed ID 26705025 |
| Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis | PubMed ID 27152709 |
| Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury | PubMed ID 29188738 |
| Novel therapeutic approach by nicotine in experimental model of multiple sclerosis | PubMed ID 23696955 |
| The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells | PubMed ID 30010837 |
| Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026 | PubMed ID 25412187 |
| Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026 | PubMed ID 25412187 |
| The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease | PubMed ID 28895883 |
| Baicalin attenuates alcoholic liver injury through modulation of hepatic oxidative stress, inflammation and sonic hedgehog pathway in rats | PubMed ID 27576501 |
| Bradykinin stimulates IL-6 production and cell invasion in colorectal cancer cells. | PubMed ID 25109623 |
| The Impact of Pre-Cooling and CoQ 10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers | PubMed ID 31094229 |
| Evaluation of second trimester amniotic fluid ADAMTS4, ADAMTS5, interleukin-6 and tumor necrosis factor-α levels in patients with gestational diabetes mellitus. | PubMed ID 30623540 |
| Serum Interleukin-6 is Not Linked with Sleep-Quality, Restless Legs Syndrome, and Depression, But with 6-Month Survival in Hematological Malignancies. | PubMed ID 30765978 |
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Questions and answers from customers related to EK0410 Human IL-6 ELISA Kit PicoKine™
3 Related Questions
Question
Q: What are some alternative names that could be used to describe this product?
Verified Customer
Asked: 0000-00-00
Answer
A: Some common names include but are not limited to human il 6 elisa kit, human il-6 elisa kit, human il6 elisa kit, human interleukin 6 elisa kit, human interleukin-6 elisa kit
Boster Scientific Support
Answered: 0000-00-00
Question
Q: What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly. Keyword: protocol, applications
Verified Customer
Asked: 0000-00-00
Answer
A: 0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.
Boster Scientific Support
Answered: 0000-00-00
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