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Product Info Summary
| SKU: | EK0410 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
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Product info
Product Name
Human IL-6/Interleukin-6 ELISA Kit PicoKine®
SKU/Catalog Number
EK0410
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-6/Interleukin-6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: P29-M212
Sensitivity
<0.3 pg/ml
Assay Range
4.69 pg/ml - 300 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0410 is reactive to IL6 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0410 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-6
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0410-CAP | Anti-Human IL6 Pre-coated 96-well strip microplate | 1 |
| EK0410-ST | Human IL6 Standard | 2 vials, 10 ng/tube |
| EK0410-DA | Human IL6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.031 | 0.091 | 0.127 | 0.222 | 0.513 | 0.749 | 1.186 | 1.997 |
Data Example Images
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Human IL-6 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-6, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IL-6 in samples: 24401617 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-6/Interleukin-6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
| Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
| CV (%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-6 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 12 | 11 | 10 | 10 | 0.82 | 8.2% |
| Sample 2 | 38 | 43 | 40 | 42 | 40 | 1.92 | 4.8% |
| Sample 3 | 145 | 149 | 145 | 150 | 147 | 2.27 | 1.5% |
Protein Target Info & Infographic
Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)
Uniprot ID
P05231
Gene ID
3569
Gene Name
IL6
Full Name
Interleukin-6
Weight
23.718kDa
Superfamily
IL-6 superfamily
Alternative Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0410)
EK0410 has been cited in 471 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The effect of the surgical microscope on the outcome of root scaling
The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury
Identification of genes involved in enterovirus 71 infected SK-N-SH cells
Vaccarin prevents ox-LDL-induced HUVEC EndMT, inflammation and apoptosis by suppressing ROS/p38 MAPK signaling
Research on the correlation between the fibrinogen β and attack of pediatric pneumonia.
Correlation between intestinal flora and serum inflammatory factors in patients with Crohns disease.
Inflammatory factor in donor liver and its effect on recipient myocardial injury after liver transplantation.
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery
Serum 17-OH progesterone and free testosterone levels in women patients with Familial Mediterranean Fever: a pivotal study.
Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
Correlation between hepcidin level and renal anemia.
Porcine parvovirus nonstructural protein NS1 activates NF-κB and it involves TLR2 signaling pathway
Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis
Hyperbaric oxygen on rehabilitation of brain tumors after surgery and effects on TNF-α and IL-6 levels
Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis
Construction of chimeric antigen receptor‑modified T cells targeting EpCAM and assessment of their anti‑tumor effect on cancer cells
LPS‑induced proinflammatory cytokine expression in human airway epithelial cells and macrophages via NF‑κB, STAT3 or AP‑1 activation
Pitavastatin attenuates monocyte activation in response to orthopedic implant‑derived wear particles by suppressing the NF‑κB signaling pathway
Inhibition of miR‑101‑3p protects against sepsis‑induced myocardial injury by inhibiting MAPK and NF‑κB pathway activation via the upregulation of DUSP1
Resveratrol inhibits the IL-1β-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and -independent signaling cascades
A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-α activity
Mechanisms of the lipopolysaccharide‑induced inflammatory response in alveolar epithelial cell/macrophage co‑culture
Differential clinical benefits of continuous blood purification treatment in critically ill patients with variable APACHE II scores
Atorvastatin reverses the dysfunction of human umbilical vein endothelial cells induced by angiotensin II
The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis
The role and mechanism of NF-κB in viral encephalitis of children
Morphological and functional changes in bone marrow mesenchymal stem cells in rats with heart failure
Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery
Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
NR4A1 is associated with chronic low‑grade inflammation in patients with type 2 diabetes
Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes
Anti-Inflammatory Property of the Essential Oil from Cinnamomum camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis
Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma
A Combination of Celecoxib and Glucosamine Sulfate Has Anti-Inflammatory and Chondroprotective Effects: Results from an In Vitro Study on Human Osteoarthritic Chondrocytes
Impact of Leptin on Periodontal Ligament Fibroblasts during Mechanical Strain
Impact of Mechanical Load on the Expression Profile of Synovial Fibroblasts from Patients with and without Osteoarthritis
C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells
Metabolic Differences between Subcutaneous and Visceral Adipocytes Differentiated with an Excess of Saturated and Monounsaturated Fatty Acids
CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Aβ-Exposed Human Cortical Astrocytes
Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations
TMT-Based Plasma Proteomics Reveals Dyslipidemia Among Lowlanders During Prolonged Stay at High Altitudes
Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression
Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis
SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs via Up-Regulating GNAI2 and PCBP1
Trichomicin Suppresses Colorectal Cancer via Comprehensive Regulation of IL-6 and TNFα in Tumor Cells, TAMs, and CAFs
Caffeic Acid Phenethyl Ester Prevents Colitis-Associated Cancer by Inhibiting NLRP3 Inflammasome
FoxP3 - Tr1 Cell in Generalized Myasthenia Gravis and Its Relationship With the Anti-AChR Antibody and Immunomodulatory Cytokines
Anti-inflammatory Effect of Curcumin, Homotaurine, and Vitamin D3 on Human Vitreous in Patients With Diabetic Retinopathy
Association of Olfactory Impairment and Postoperative Cognitive Dysfunction in Elderly Patients
Screening, Safety Evaluation, and Mechanism of Two Lactobacillus fermentum Strains in Reducing the Translocation of Staphylococcus aureus in the Caco-2 Monolayer Model
Elevated synovial fluid IL-33 and IL-6 levels and cartilage degeneration in stage III osteonecrosis of the femoral head
What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?
Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease
Serum cytokine and chemokine profiles in patients with alopecia areata
Distinct peritoneal fluid ghrelin and leptin in infertile women with endometriosis and their correlation with interleukin-6 and vascular endothelial growth factor
Can serum NGAL levels be used as an inflammation marker on hemodialysis patients with permanent catheter?
Analysis of IL-10 and IL-6 Gene Polymorphisms and Their Serum Levels in Patients with Brucellosis: A Case Control Study
Eudragit nanoparticles loaded with silybin: a detailed study of preparation, freeze-drying condition and in vitro/in vivo evaluation
RETRACTED ARTICLE: Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
MiR-140a contributes to the pro-atherosclerotic phenotype of macrophages by downregulating interleukin-10.
LncRNA MEG3 inhibits proliferation and promotes apoptosis of osteosarcoma cells through regulating Notch signaling pathway.
High glucose promotes prostate cancer cells apoptosis via Nrf2/ARE signaling pathway.
Effects of Additional Intraoperative Administration of Sufentanil on Postoperative Pain, Stress and Inflammatory Responses in Patients Undergoing Laparoscopic Myomectomy: A Double-Blind, Randomized, Placebo-Controlled Trial
Investigation of Association of Complement 5 Genetic Polymorphisms with Sepsis and Sepsis-Induced Inflammatory Responses
Platelet-rich plasma plays an antibacterial, anti-inflammatory and cell proliferation-promoting role in an in vitro model for diabetic infected wounds
Resveratrol Exerts Anti-Osteoarthritic Effect by Inhibiting TLR4/NF-κB Signaling Pathway via the TLR4/Akt/FoxO1 Axis in IL-1β-Stimulated SW1353 Cells
Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study
The activation of IL-17 signaling pathway promotes pyroptosis in pneumonia-induced sepsis
The effect of red blood cell transfusion on plasma hepcidin and growth differentiation factor 15 in gastric cancer patients: a prospective study.
Chemerin as a Novel Crevicular Fluid Marker of Patients With Periodontitis and Type 2 Diabetes Mellitus
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide
Ferulic Acid Mitigates Radiation Injury in Human Umbilical Vein Endothelial Cells In Vitro via the Thrombomodulin Pathway
Associations between second-trimester amniotic fluid levels of ADAMTS4, ADAMTS5, IL-6, and TNF-α and spontaneous preterm delivery in singleton pregnancies
Alkaloids of Abuta panurensis Eichler: In silico and in vitro study of acetylcholinesterase inhibition, cytotoxic and immunomodulatory activities
Hyaluronic acid and chondroitin sulfate, alone or in combination, efficiently counteract induced bladder cell damage and inflammation
Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers
TNFα-Mediated Loss of β-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells
Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-κB/COX-2 Pathway
Xanthan Gum Ameliorates Osteoarthritis and Mitigates Cartilage Degradation via Regulation of the Wnt3a/β-Catenin Signaling Pathway
Effects of Dexmedetomidine Combined with Sufentanil on Postoperative Delirium in Young Patients After General Anesthesia
Role of Different Blood Purification Nursing Models in Uremic Patients: A Preliminary Report
The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response
MLL1, a H3K4 methyltransferase, regulates the TNFα-stimulated activation of genes downstream of NF-κB
The effect of omega-3 fatty acids on clinical and paraclinical features of intractable epileptic patients: a triple blind randomized clinical trial
Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response
Effect of rosiglitazone on inflammatory cytokines and oxidative stress after intensive insulin therapy in patients with newly diagnosed type 2 diabetes
miR-449a inhibits cell proliferation, migration, and inflammation by regulating high-mobility group box protein 1 and forms a mutual inhibition loop with Yin Yang 1 in rheumatoid arthritis fibroblast-like synoviocytes
Circ_0045714/miR-331-3p interaction affects IL-1β-evoked human articular chondrocyte injury through regulating PIK3R3 in a ceRNA regulatory cascade
miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40
Experimental validation and computational modeling of anti-influenza effects of quercetin-3-O-α-L-rhamnopyranoside from indigenous south African medicinal plant Rapanea melanophloeos
Relationship between inflammatory markers and mild cognitive impairment in Chinese patients with type 2 diabetes: a case-control study
The IL-6 rs12700386 polymorphism is associated with an increased risk of developing osteoarthritis in the knee in the Chinese Han population: a case-control study
Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome
Chicoric acid alleviates LPS-induced inflammatory response through miR-130a-3p/IGF-1pathway in human lung A549 epithelial cells:
Interleukin-6 serum level and -597 A/G gene polymorphism in moderate and severe chronic obstructive pulmonary disease:
Expression of S100 calcium-binding protein A8 in peripheral blood of patients with preeclampsia during pregnancy:
LncRNA CASC2 is involved in the development of chronic obstructive pulmonary disease via targeting miR-18a-5p/IGF1 axis:
Effect of curcumin on lung epithelial injury and ferroptosis induced by cigarette smoke:
Lipopolysaccharide induces epithelial–mesenchymal transition of alveolar epithelial cells cocultured with macrophages possibly via the JAK2/STAT3 signaling pathway:
Epithelial Microvesicles Promote an Inflammatory Phenotype in Fibroblasts:
The Effect of Autophagic Activity on the Function of Apheresis Platelets and on the Efficacy of Clinical Platelet Transfusion
Induction of Pro-Inflammatory Response via Activated Macrophage-Mediated NF-κB and STAT3 Pathways in Gastric Cancer Cells
Low Oxygen Consumption is Related to a Hypomethylation and an Increased Secretion of IL-6 in Obese Subjects with Sleep Apnea-Hypopnea Syndrome
Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine
Anti-Inflammatory Effect of White Wine in CKD Patients and Healthy Volunteers
Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast
Chronic Hypoxia-Induced Microvessel Proliferation and Basal Membrane Degradation in the Bone Marrow of Rats Regulated through the IL-6/JAK2/STAT3/MMP-9 Pathway
Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Cell Invasion through Integrin β6 Upregulation in Colorectal Cancer
A Novel Putative Role of TNK1 in Atherosclerotic Inflammation Implicating the Tyk2/STAT1 Pathway
Ginsenoside Rg1 Regulates SIRT1 to Ameliorate Sepsis-Induced Lung Inflammation and Injury via Inhibiting Endoplasmic Reticulum Stress and Inflammation
Can Inflammatory and Nutritional Serum Markers Predict Chemotherapy Outcomes and Survival in Advanced Stage Nonsmall Cell Lung Cancer Patients?
Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet’s Disease
NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation
Placental Growth Factor, Soluble fms-Like Tyrosine Kinase 1, Soluble Endoglin, IL-6, and IL-16 as Biomarkers in Preeclampsia
GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by Mycobacterium avium-Infected Macrophages
Inhibition of EZH2 attenuates coronary heart disease by interacting with microRNA‐22 to regulate the TXNIP/nuclear factor‐κB pathway
Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross‐sectional, single‐center study in the USA
Anti‐inflammatory effect of paeoniflorin combined with baicalin in oral inflammatory diseases
Role of mammary serine protease inhibitor on the inflammatory response in oral lichen planus
Chemerin and interleukin‐6 levels in obese individuals following periodontal treatment
LIPUS inhibited the expression of inflammatory factors and promoted the osteogenic differentiation capacity of hPDLCs by inhibiting the NF‐κB signaling pathway
Evaluation of second trimester amniotic fluid ADAMTS4, ADAMTS5, interleukin‐6 and tumor necrosis factor‐α levels in patients with gestational diabetes mellitus
miR‐499 released during myocardial infarction causes endothelial injury by targeting α7‐nAchR
SP/NK‐1R promotes gallbladder cancer cell proliferation and migration
5,2′‐dibromo‐2,4′,5′‐trihydroxydiphenylmethanone attenuates LPS‐induced inflammation and ROS production in EA.hy926 cells via HMBOX1 induction
Pannexin3 inhibits TNF‐α‐induced inflammatory response by suppressing NF‐κB signalling pathway in human dental pulp cells
Associations between Dietary Intake of B-vitamins and Cardiovascular Risk Factors in Elderly Men: A Cross-Sectional Study
The antioxidant and anti‐inflammatory effects of astaxanthin supplementation on the expression of miR‐146a and miR‐126 in patients with type 2 diabetes mellitus: A randomised, double‐blind, placebo‐controlled clinical trial
Effects of short‐term xylitol chewing gum on pro‐inflammatory cytokines and Streptococcus mutans: A randomised, placebo‐controlled trial
MicroRNA profiling in Chinese children with Henoch‐Schonlein purpura and association between selected microRNAs and inflammatory biomarkers
Therapeutic effect of inhaled budesonide (Pulmicort® Turbuhaler) on the inflammatory response to one‐lung ventilation
Association of the Elderly Dietary Index with cardiovascular disease risk factors in elderly men: A cross‐sectional study
Soy milk consumption, markers of inflammation and oxidative stress in women with rheumatoid arthritis: A randomised cross‐over clinical trial
Effects of Different Blood Purification Methods on Serum Cytokine Levels and Prognosis in Patients With Acute Severe Organophosphorus Pesticide Poisoning
Knockdown of CTRP6 inhibits high glucose‐induced oxidative stress, inflammation and extracellular matrix accumulation in mesangial cells through regulating the Akt/NF‐κB pathway
Down‐regulation of microRNA‐375 regulates adipokines and inhibits inflammatory cytokines by targeting AdipoR2 in non‐alcoholic fatty liver disease
Chlamydia trachomatis inhibits the production of pro-inflammatory cytokines in human PBMCs through induction of IL-10
Obesity is a risk factor for preoperative hypoxemia in Stanford A acute aortic dissection
CircSCAP Aggravates Oxidized Low-density Lipoprotein-induced Macrophage Injury by Upregulating PDE3B by miR-221-5p in Atherosclerosis
Circular RNA 0039411 Is Involved in Neodymium Oxide-induced Inflammation and Antiproliferation in a Human Bronchial Epithelial Cell Line via Sponging miR-93-5p
Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study)
High-volume online haemodiafiltration improves erythropoiesis-stimulating agent (ESA) resistance in comparison with low-flux bicarbonate dialysis: results of the REDERT study
The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells
Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms
The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway
Effects of A94T and P84L Polymorphisms Within the TNF-α Gene on Proliferation and Activation of Hepatic Stellate Cells
Chronic Inflammation in a Long-Term Cohort of HIV-Infected Patients According to the Normalization of the CD4:CD8 Ratio
RETRACTED ARTICLE: MicroRNA-210 protects against periodontitis through targeting HIF-3α and inhibiting p38MAPK/NF-κB pathway
The effect of vitamin D and calcium supplementation on inflammatory biomarkers, estradiol levels and severity of symptoms in women with postpartum depression: a randomized double-blind clinical trial
Evaluation of pro-oxidant-antioxidant balance (PAB) and its association with inflammatory cytokines in polycystic ovary syndrome (PCOS)
Obesity and ultrasound-estimated visceral fat deposits in women undergoing Assisted Reproductive Technology (ART) procedures
Th17/Treg-related cytokine imbalance in sulfur mustard exposed and stable chronic obstructive pulmonary (COPD) patients: correlation with disease activity
Clock gene PERIOD3 polymorphism is associated with susceptibility to Graves’ disease but not to Hashimoto’s thyroiditis
Effect of Low-Energy-Dense Diet Rich in Multiple Functional Foods on Weight-Loss Maintenance, Inflammation, and Cardiovascular Risk Factors: A Randomized Controlled Trial
The Impact of Pre-Cooling and CoQ10 Supplementation on Mediators of Inflammatory Cytokines in Elite Swimmers
Clinical features and monocyte/macrophage subsets characterization in granulomatous vs non-granulomatous Crohn’s disease
Dysregulation of miR-192-5p in acute pancreatitis patients with nonalcoholic fatty liver and its functional role in acute pancreatitis progression
Extracts of Thai Perilla frutescens nutlets attenuate tumour necrosis factor-α-activated generation of microparticles, ICAM-1 and IL-6 in human endothelial cells
MicroRNA-451 inhibits inflammation and proliferation of glomerular mesangial cells through down-regulating PSMD11 and NF-κB p65
Long non-coding RNA TUG1 alleviates high glucose induced podocyte inflammation, fibrosis and apoptosis in diabetic nephropathy via targeting the miR-27a-3p/E2F3 axis
Epigallocatechin gallate prevents senescence by alleviating oxidative stress and inflammation in WI-38 human embryonic fibroblasts
Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo
Resistant starch type-2 enriched cookies modulate uremic toxins and inflammation in hemodialysis patients: a randomized, double-blind, crossover and placebo-controlled trial
Could resistant starch supplementation improve inflammatory and oxidative stress biomarkers and uremic toxins levels in hemodialysis patients? A pilot randomized controlled trial
A gain-of-function NLRP3 3′-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression
Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction
Oral immune dysfunction is associated with the expansion of FOXP3+PD-1+Amphiregulin+ T cells during HIV infection
Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer
Altered gut microbiota after traumatic splenectomy is associated with endotoxemia
Chimeric antigen receptor T cells self-neutralizing IL6 storm in patients with hematologic malignancy
Keratinocyte Microvesicles Regulate the Expression of Multiple Genes in Dermal Fibroblasts
Improvement of hypertension, endothelial function and systemic inflammation following short-term supplementation with red beet ( Beta vulgaris L.) juice: a randomized crossover pilot study
Bclaf1 is an important NF- κ B signaling transducer and C/EBP β regulator in DNA damage-induced senescence
Microvesicles secreted from human multiple myeloma cells promote angiogenesis
Immune Profiling of Polysaccharide Submicron Vesicles
Plasma redox and inflammatory patterns during major depressive episodes: a cross-sectional investigation in elderly patients with mood disorders
Association between increased serum interleukin-6 levels and sustained attention deficits in patients with major depressive disorder
Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment
Antibacterial Fusion Protein BPI21/LL-37 Modification Enhances the Therapeutic Efficacy of hUC-MSCs in Sepsis
Tumor-associated macrophages induce PD-L1 expression in gastric cancer cells through IL-6 and TNF-ɑ signaling
Porcine parvovirus infection activates inflammatory cytokine production through Toll-like receptor 9 and NF-κB signaling pathways in porcine kidney cells
CIRCULATING MIR-140 AND LEPTIN IMPROVE THE ACCURACY OF THE DIFFERENTIAL DIAGNOSIS BETWEEN PSORIATIC ARTHRITIS AND RHEUMATOID ARTHRITIS: A CASE-CONTROL STUDY
LncRNA LOC101927514 regulates PM2.5-driven inflammation in human bronchial epithelial cells through binding p-STAT3 protein
Nitrogen mustard prevents transport of Fra-1 into the nucleus to promote c-Fos- and FosB-dependent IL-8 induction in injured mouse epidermis
miR-503/Apelin-12 mediates high glucose-induced microvascular endothelial cells injury via JNK and p38MAPK signaling pathway
C1q/TNF-related protein 5 contributes to diabetic vascular endothelium dysfunction through promoting Nox-1 signaling
CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation
Anti-inflammatory and anti-arthritic effects of the ethanolic extract of Aralia continentalis Kitag. in IL-1β-stimulated human fibroblast-like synoviocytes and rodent models of polyarthritis and nociception
The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts
Effect of non-soy legume consumption on inflammation and serum adiponectin levels among first-degree relatives of patients with diabetes: A randomized, crossover study
Lipopolysaccharide enhances human herpesvirus 1 replication and IL-6 release in epithelial cells
Fast screening of biological fluids for cytokines and adipokines using stochastic sensing
Oriented co-continuous 3D porous scaffolds with inhibited activating functionality: An effective strategy to inhibit the hyperactivation of astrocytes
MiR-424 overexpression protects alveolar epithelial cells from LPS-induced apoptosis and inflammation by targeting FGF2 via the NF-κB pathway
The shift of macrophages toward M1 phenotype promotes aortic valvular calcification
Penehyclidine hydrochloride preserves the intestinal barrier function in patients undergoing cardiopulmonary bypass
Effective-constituent compatibility-based analysis of Bufei Yishen formula, a traditional herbal compound as an effective treatment for chronic obstructive pulmonary disease
The role of human herpesvirus-6 and inflammatory markers in the pathogenesis of multiple sclerosis
The protective effect of Helicobacter Pylori infection on the susceptibility of multiple sclerosis
Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine
High throughput data-based, toxicity pathway-oriented development of a quantitative adverse outcome pathway network linking AHR activation to lung damages
Significant association of rheumatoid arthritis-related inflammatory markers with non-surgical periodontal therapy
Oxidative Stress Associated With Spasmodic, Flatulent, and Impaction Colic in Draft Horses
Protective effects of organic acid component from Taraxacum mongolicum Hand.-Mazz. against LPS-induced inflammation: Regulating the TLR4/IKK/NF-κB signal pathway
TAp63 is correlated with chronic inflammation in patients with newly diagnosed type 2 diabetes mellitus
Effect of propofol, sevoflurane, and isoflurane on postoperative cognitive dysfunction following laparoscopic cholecystectomy in elderly patients: A randomized controlled trial
Peripheral proinflammatory cytokines in Chinese patients with generalised anxiety disorder
Plumbagin suppresses the human large cell lung cancer cell lines by inhibiting IL-6/STAT3 signaling in vitro
Polysaccharide from Ephedra sinica Stapf inhibits inflammation expression by regulating Factor-β1/Smad2 signaling
The evolving role of protein disulfide isomerase A3 in Egyptian bladder cancer patients
Wogonin, a plant derived small molecule, exerts potent anti-inflammatory and chondroprotective effects through the activation of ROS/ERK/Nrf2 signaling pathways in human Osteoarthritis chondrocytes
“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model
The bidirectional crosstalk between metastatic uveal melanoma cells and hepatic stellate cells engenders an inflammatory microenvironment
Imiquimod-loaded nanocapsules improve cytotoxicity in cervical cancer cell line
MiR-140-5p/TLR4 /NF-κB signaling pathway: Crucial role in inflammatory response in 16HBE cells induced by dust fall PM2.5
NCAPG2 overexpression promotes hepatocellular carcinoma proliferation and metastasis through activating the STAT3 and NF-κB/miR-188-3p pathways
In vivo effects of methamphetamine on HIV-1 replication: A population-based study
Epigallocatechin gallate improves insulin resistance in HepG2 cells through alleviating inflammation and lipotoxicity
Effect of combined exercise training on pentraxins and pro- inflammatory cytokines in people with multiple sclerosis as a function of disability status
Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines
Determination of cytokine levels in multiple sclerosis patients and their relevance with patients response to Cinnovex
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis
Folic acid and vitamin B12 supplementation in subjects with type 2 diabetes mellitus: A multi-arm randomized controlled clinical trial
Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation
The Effect of Puerarin on Carotid Intima-media Thickness in Patients With Active Rheumatoid Arthritis: A Randomized Controlled Trial
Serum immune biomarkers in irritable bowel syndrome
Design, synthesis, and evaluation of 2-piperidone derivatives for the inhibition of β-amyloid aggregation and inflammation mediated neurotoxicity
A new compound Trichomicin exerts antitumor activity through STAT3 signaling inhibition
Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody
The synergistic function of miR-140–5p and miR-146a on TLR4-mediated cytokine secretion in osteoarthritic chondrocytes
Effects of SSRIs on peripheral inflammatory cytokines in patients with Generalized Anxiety Disorder
Cardiovascular risk in advanced naïve HIV-infected patients starting antiretroviral therapy: Comparison of three different regimens - PREVALEAT II cohort
C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway
Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells
Elevated serum neopterin levels in children with functional constipation: association with systemic proinflammatory cytokines
Comparative Analyses of Pharmaceuticals or Food Supplements Containing Chondroitin Sulfate: Are Their Bioactivities Equivalent?
The Association of Pre-diagnostic Inflammatory Markers and Adipokines and the Risk of Non-Hodgkin Lymphoma Development in Egypt
MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis
Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells
Deregulated miR-384 serves as a biomarker in neonatal hypoxic-ischemic encephalopathy and alleviates microglia-mediated neuroinflammation
Circ_0057583 facilitates brain microvascular endothelial cell injury through modulating miR-204-5p/NR4A1 axis
Adipose tissue extract shows potential for wound healing: in vitro proliferation and migration of cell types contributing to wound healing in the presence of adipose tissue preparation and platelet rich plasma
Association of serum levels of intercellular adhesion molecule-1 and interleukin-6 with migraine
Iguratimod dose dependently inhibits the expression of citrullinated proteins and peptidylarginine deiminases 2 and 4 in neutrophils from rheumatoid arthritis patients
Effects of ezetimibe and anticoagulant combined therapy on progressing stroke: a randomized, placebo-controlled study
Interleukin-6 and other inflammatory markers in diagnosis of periprosthetic joint infection
Elevated level of peripheral CD8 + CD28 − T lymphocytes are an independent predictor of progression-free survival in patients with metastatic breast cancer during the course of chemotherapy
MicroRNA‐17‐5p restrains the dysfunction of Ang‐II induced podocytes by suppressing secreted modular calcium‐binding protein 2 via NF‐κB and TGFβ signaling
Stromal cells from perinatal and adult sources modulate the inflammatory immune response in vitro by decreasing Th1 cell proliferation and cytokine secretion
Targeting inflammation-associated AMPK//Mfn-2/MAPKs signaling pathways by baicalein exerts anti-atherosclerotic action
Eriodictyol inhibits high glucose‐induced extracellular matrix accumulation, oxidative stress, and inflammation in human glomerular mesangial cells
Clinical Evaluation of Blood Pressure Lowering, Endothelial Function Improving, Hypolipidemic and Anti‐Inflammatory Effects of Pomegranate Juice in Hypertensive Subjects
Wogonoside impedes the progression of acute myeloid leukemia through inhibiting bone marrow angiogenesis
An advanced tri‐culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells
A Human 3D In Vitro Model to Assess the Relationship Between Osteoporosis and Dissemination to Bone of Breast Cancer Tumor Cells
The Relationship Among the Level of Serum Amyloid A, High‐Density Lipoprotein and Microalbuminuria in Patients With Familial Mediterranean Fever
Carvacrol suppresses inflammatory responses in rheumatoid arthritis fibroblast‐like synoviocytes
Methylene blue regulates inflammatory response in osteoarthritis by noncoding long chain RNA CILinc02
MEG3 aggravates hypoxia/reoxygenation induced apoptosis of renal tubular epithelial cells via the miR‐129‐5p/HMGB1 axis
LncRNA PVT1 exacerbates the inflammation and cell‐barrier injury during asthma by regulating miR‐149
Euxanthone inhibits lipopolysaccharide-induced injury, inflammatory response, and MUC5AC hypersecretion in human airway epithelial cells by the TLR4/MyD88 pathway
NLRP3 expression and urinary HSP72 in relation to biomarkers of inflammation and oxidative stress in diabetic nephropathy patients
miR‐16 exhibits protective function in LPS‐treated cardiomyocytes by targeting DOCK2 to repress cell apoptosis and exert anti‐inflammatory effect
Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL‐6/HIF‐1α signaling pathway
The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function
Species: Human
IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Species: Human
Frequency of NFKBIA deletions is low in glioblastomas and skewed in glioblastoma neurospheres
Species: Human
Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy
Species: Human
Klotho suppresses RIG-I-mediated senescence-associated inflammation
Species: Human
Lu F,Chen H,Hong Y,Lin Y,Liu L,Wei N,Wu Q,Liao S,Yang S,He J,Shao Y.A gain-of-function NLRP3 3‘-UTR polymorphism causes miR-146a-mediated suppression of NLRP3 expression and confers protection against sepsis progression.Sci Rep.2021 Jun 25;11(1):13300.doi:10.1038/s41598-021-92547-8.PMID: 34172780.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE: PLASMA,HUVECS AND THP-1 CELL, DILUTION:NA
Boyang Sun,Siqi Ying,Qian Ma,Han Li,Jie Li,Jinlin Song,Metformin ameliorates HMGB1-mediated oxidative stress through mTOR/AMPK pathway in experimental periodontitis,Genes & Diseases,2021,,ISSN 2352-3042,https://doi.org/10.1016/j.gendis.2021.06.003.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:HPDLCS, DILUTION:NA
Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION: NA
Tutuncu EO,Dundar ZD,Kilinc I,Tutuncu A,Kocak S,Girisgin AS.Prognostic Value of Immunosuppressive Acidic Protein and Oxidative Stress Status in Critically Ill Patients.Indian J Crit Care Med.2021 Apr;25(4):405-409.doi:10.5005/jp-journals-10071-23788.PMID:34045807;PMCID:PMC8138636.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA
Stellavato,A.;Restaino,O.F.;Vassallo,V.;Cassese,E.;Finamore,R.;Ruosi,C.;Schiraldi,C.Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes.Pharmaceutics 2021,13,737.https://doi.org/10.3390/pharmaceutics13050737
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:CHONDROCYTES, DILUTION:NA
Zhang X,Zhu M,Hong Z,Chen C.Co-culturing polarized M2 Thp-1-derived macrophages enhance stemness of lung adenocarcinoma A549 cells.Ann Transl Med.2021 Apr;9(8):709.doi:10.21037/atm-21-1256.PMID:33987407;PMCID:PMC8106048.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:A549 CELL, DILUTION:NA
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA
Liu T,Yin C,Li Y,Gao F,Yu L,Wang Z,Wang Q.Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study.Clin Interv Aging.2021 May 3;16:757-765.doi:10.2147/CIA.S309082.PMID:33976542;PMCID:PMC8106456.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Erica Costantini,Bruna Sinjari,Katia Falasca,Marcella Reale,Sergio Caputi,Srinivas Jagarlapodii,Giovanna Murmura," Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast",Mediators of Inflammation,vol.2021,Article ID 5562340,9 pages,2021. https://doi.org/10.1155/2021/5562340
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HGF CELL, DILUTION:NA
Pınar Bürhan-Çavuşoğlu,Evin İşcan,Ayşim Güneş,Neşe Atabey,Tunç Alkın,Increased telomerase activity in major depressive disorder with melancholic features: Possible role of pro-inflammatory cytokines and the brain-derived neurotrophic factor,Brain,Behavior,& Immunity - Health,2021,100259,ISSN 2666-3546,https://doi.org/10.1016/j.bbih.2021.100259.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Yang N,Wang H,Zhang R,Niu Z,Zheng S,Zhang Z.C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway.Arch Med Res.2021 May 1:S0188-4409(21)00079-5.doi:10.1016/j.arcmed.2021.03.008.Epub ahead of print.PMID:33947580.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA
Yi Y,Chai X,Zheng L,Zhang Y,Shen J,Hu B,Tao G.CRISPR-edited CART with GM-CSF knockout and auto secretion of IL6 and IL1 blockers in patients with hematologic malignancy.Cell Discov.2021 Apr 27;7(1):27.doi:10.1038/s41421-021-00255-4.PMID:33907185.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Yang M, Wang LI. MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis. J Biosci. 2021;46:28. PMID: 33753579.
Species: Human,Rat
EK0410 usage in article: APP:ELISA, SAMPLE:B/TSMCS, DILUTION:NA
Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021 Mar 12. doi: 10.1007/s10753-021-01445-8. Epub ahead of print. PMID: 33710445.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA
Li C, Liu M, Liu K, Li M, Liu Y, Li T, Wei Y, Long Y, He W, Shi X, Li Y, Zhang H. BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody. Biochem Biophys Res Commun. 2021 Mar 16;551:155-160. doi: 10.1016/j.bbrc.2021.02.128. Epub ahead of print. PMID: 33740622.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA
Huang C,Chen Y,Lai B,Chen YX,Xu CY,Liu YF.Overexpression of SP1 restores autophagy to alleviate acute renal injury induced by ischemia-reperfusion through the miR-205/PTEN/Akt pathway.J Inflamm (Lond).2021 Feb 5;18(1):7.doi:10.1186/s12950-021-00270-y.PMID
Species: Human,Rat
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA
Junde Hou,Yanxin Cheng,Hongfang Wei et al.Dexmedetomidine Attenuates Hemodynamic and Proinflammatory Responses During Craniotomy for Traumatic Brain Injury:A Randomized Controlled Trial Study,02 February 2021,PREPRINT (Version 1) available at Research Squ
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Qinchao Chen,Meng Cao,Hanyi Ge,"Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis",BioMed Research International,vol.2021,Article ID 8899863,12 pages,2021.https://doi.org/10.1155/2021/8899863
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA
Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA
Gao H,Guo Z. LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Med (Wars). 2021 Jan 8;16(1):117-127.doi:10.1515/med-2021-0200.PMID:33542956;PMCID: PMC7819546.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA
Wei W,Yao Y,Bi H,Xu W,Gao Y.Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.Biochem Biophys Res Commun.2021 Jan 8;540:1-7.doi:10.1016/j.bbrc.2020.12.018.Epub ahead of print.PMID:33429194.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA
Shaat, R.M.,Elsaid, T.O.,EL Baz, A. et al. Do obese patients with primary knee osteoarthritis benefit from a single bout of moderate intensity aerobic exercise?.Egypt Rheumatol Rehabil 47,27 (2020).https://doi.org/10. 1186/s43166-020-00029-y
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Fu,C.,Xu,Q.,Tang,S.,Cao,Y.,Liu,C.,Wang,Y.,Qian,Y.,Shi,F.,Gui,J.,Fan,Q., & Ling,Y.(2020).The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury.American journal of translational research, 12(11), 7114–712
Species: Human,Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
EK0410 usage in article: APP:ELISA, SAMPLE:MSCS, DILUTION:NA
Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865
Species: Human, Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Liao H,Zhang H,Xiang J,Chen G,Cao Z.The effect of the surgical microscope on the outcome of root scaling. Am J Transl Res. 2020 Nov 15;12(11):7199-7210.PMID: 33312360;PMCID:PMC7724328.
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA
Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov
Species: Human
Xue M, Peng N, Zhu X, Zhang H. Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis. Biochem Biophys Res Commun. 2020 Nov 25:S0006-291X(20)32086-6. doi
Species: Human
Asila A, Wang Y, Miao D, Yang X, Kaisaer Y, Ma L, Lv G, Yang C. SNHG16/miR-485-5p/BMP7 axis modulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Gene Med. 2020 Nov 11:e3296. doi: 10.1002/jgm.3296. Epub ahead of print
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:HBMSCS, DILUTION:NA
Yuan X, Zhang L, Huang Y, Liu D, Peng P, Liu S, Long G, Hu G, Sun W. Induction of interleukin-6 by irradiation and its role in epithelial mesenchymal transition and radioresistance of nasopharyngeal carcinoma cells. Head Neck. 2020 Nov 5. doi: 10.1002/hed
Species: Human
EK0410 usage in article: APP:ELISA, SAMPLE:NPC CELLS, DILUTION:NA
Hu Q, Peng J, Jiang L, Li W, Su Q, Zhang J, Li H, Song M, Cheng B, Xia J, Wu T. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis. 2020 Oct 28;11(10):925. doi: 10.1
Species: Mouse
EK0410 usage in article: APP:ELISA, SAMPLE:CAL 27 AND HSC6 CELL LINES, DILUTION:NA
Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.
Serum Interleukin-6 is Not Linked with Sleep-Quality, Restless Legs Syndrome, and Depression, But with 6-Month Survival in Hematological Malignancies.
Evaluation of second trimester amniotic fluid ADAMTS4, ADAMTS5, interleukin-6 and tumor necrosis factor-α levels in patients with gestational diabetes mellitus.
The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease
Novel therapeutic approach by nicotine in experimental model of multiple sclerosis
Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026
E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome
Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine
Establishment of a Rabbit Model of Chronic Obstructive Sleep Apnea and Application in Cardiovascular Consequences
Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes
Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis
The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis
Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial
Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis
Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients
Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice
Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression
Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells
Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery
A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes
Placental Growth Factor, Soluble fms-Like Tyrosine Kinase 1, Soluble Endoglin, IL-6, and IL-16 as Biomarkers in Preeclampsia
Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage
Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells
Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial
Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis
LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling
Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease
miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40
NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation.
Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway
Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome
Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP)
Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes
GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway
Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells
C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells
Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis
Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide
Association of serum immunoglobulin-G to Porphyromonas gingivalis with acute cerebral infarction in the Chinese population
Bclaf1 is an important NF-?B signaling transducer and C/EBP? regulator in DNA damage-induced senescence
IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
miR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes
Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages
Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery
TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells
Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms
RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms
A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-? activity
Evaluation of cytokines as Th1/Th2 markers in pathogenesis of children with Crimean-Congo hemorrhagic fever
Microvesicles secreted from human multiple myeloma cells promote angiogenesis
Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients
X4 viruses are frequently archived in patients with long-term HIV infection but do not seem to influence the ?inflamm-aging? process
Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis
Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy
Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-?B/COX-2 Pathway
Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome
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Customer Q&As
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20 Customer Q&As for Human IL-6/Interleukin-6 ELISA Kit PicoKine®
Question
Is EK0410 valid for human milk samples?
Verified customer
Asked: 2022-03-30
Answer
Human IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0410) has not been tested on human milk explicitly. In our experience customers have used it successfully in human milk. Thus we are willing to extend our quality guarantee to cover human milk as a viable sample type.
Boster Scientific Support
Answered: 2022-03-30
Question
Is EK0410 still usable if left at room temperature without cooling for 2 days?
Verified customer
Asked: 2020-06-18
Answer
The Human IL-6 ELISA Kit PicoKine™ (EK0410) should be stable but it is not covered by warranty if not stored according to instructions.
Boster Scientific Support
Answered: 2020-06-18
Question
Hello Support: I purchased Human IL-6 ELISA Kit but I have some questions, could you please give advice on staining technique? Is it possible to use only few wells for analysis of several samples and save other plates for further studies? Lastly, do I need to buy any software for data analysis, or Excel will be enough?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The Human IL-6 ELISA Kit comes with 96 well with removable strips, so you can remove as many strips as you need before using it, then store the remaining strips in the pouch with desiccant, sealed it tightly and put in storage at 4C. For your data analysis excel will do. All you need to do is to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve.
Boster Scientific Support
Answered: 2020-04-30
Question
Q: can I use heparin plasma as samples in Human IL-6 Picokine® ELISA Kit (Catalog # EK0410)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-6 causing disruption of its protein structure. IL-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-6 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-20
Question
What is the expiration date of EK0410 from the time of manufacturing, 6 months or 1 year?
Verified customer
Asked: 2019-12-17
Answer
Expiration is based on storage temperature. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Boster Scientific Support
Answered: 2019-12-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-22
Question
I purchased Human IL-6 ELISA Kit and I reconstitute with acetic acid and the coating buffer is alkaline solution of pH 9.6. I am concern with the pH change and possibly affects my results? Please let me know
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We recommend to use for coating buffers, the standard PBS rather than alkaline carbonate buffers for this antibody Human IL-6 ELISA Kit
Boster Scientific Support
Answered: 2019-08-30
Question
Is the Human IL-6 ELISA Kit have been tested with saliva samples? Do you have any suggestions?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The Human IL-6 ELISA Kit is suitable to use in saliva samples and the usual protocol would apply. No need for pre-treatment of sample before assay.
Boster Scientific Support
Answered: 2019-08-15
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-04
Question
What is the normal serum range of EK0410?
Verified customer
Asked: 2019-04-08
Answer
Please see the normal serum range for the Human IL-6 ELISA Kit PicoKine™ (EK0410) below; IL-6 (Catlog no. EK0410), 0-15pg/mL
Boster Scientific Support
Answered: 2019-04-09
Question
Is it normal to have fluid in the well of the EK0410 plate and a solid standard that is very hard to dissolve.
Verified customer
Asked: 2019-04-01
Answer
For the Human IL-6 ELISA Kit PicoKine™ (EK0410), it is normal that there is fluid in the wells of the plate, and the drops could be removed by patting the plate on a paper towel or by aspiration. For the standard, we never encountered this problem before, and our technicians suggests to centrifuge at 2000-4000×g for 5 minutes after dissolving the standard.
Boster Scientific Support
Answered: 2019-04-02
Question
Have you tested EK0410 and EK0416 in tissue homogenates and Cell culture supernates?
Verified customer
Asked: 2019-03-15
Answer
The Human IL-6 EK0410 Elisa Kit and Human IL-10 EK0416 Elisa Kit have not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-6 using the EK0410 Human IL-6 Picokine® ELISA Kit?
E. Puri
Verified customer
Asked: 2019-03-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-03-11
Question
Please let me know if you CAN supply QC materials for EACH of these kits?. If you could introduce in EACH kit at least 2 or 3 vials with QC material (having 2 or 3 different concentrations of the analyte for which the kits are used) it would be PERFECT! (even if the raw materials of these QC vials will be the same as the standards
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
We can not provide 2 or 3 different concentrations of the analyte(e.g. serum) for those kits. The QC vials we can provide are recombinant proteins of different concentrtaions.
Boster Scientific Support
Answered: 2019-02-12
Question
Does EK0410 work with whole cell lysates?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
EK0410 is not suitable for working with whole cell lysates.
Boster Scientific Support
Answered: 2018-06-29
Question
Have you tested this kit with pig samples? Keyword: applications, porcine, detection
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
No, we have not tested this with pig samples. A pilot experiment is recommended first if you plan to detect pig samples.
Boster Scientific Support
Answered: 2018-02-12
Question
What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly. Keyword: protocol, applications
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.
Boster Scientific Support
Answered: 2017-09-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Harris
Verified customer
Asked: 2017-07-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-07-23
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-6 level in plasma.
Verified Customer
Verified customer
Asked: 2016-07-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-29
Question
We received an inquiry regarding the EK0410 IL-6 ELISA Kit (Human) from a customer:
Verified Customer
Verified customer
Asked: 2016-01-06
Answer
<0.3pg/ml *The sensitivity or the minimum detectable dose (MDD) is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration.
Boster Scientific Support
Answered: 2016-01-06