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Human IL-6/Interleukin-6 ELISA Kit PicoKine™
Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).. Sensitivity: 0.3pg/ml. Cited in 191 publication(s).
Human IL-6/Interleukin-6 ELISA Kit PicoKine™ Product Summary
| SKU: | EK0410 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <0.3 pg/ml |
| Assay Range: | 4.69 pg/ml - 300 pg/ml |
Overview
| Product Name | Human IL-6/Interleukin-6 ELISA Kit PicoKine™
See more IL-6 products |
|---|---|
| Catalog# | EK0410 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).. Sensitivity: 0.3pg/ml. |
| Cite This Product | Human IL-6/Interleukin-6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0410) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
| Sensitivity | <0.3 pg/ml |
| Assay Range | 4.69 pg/ml - 300 pg/ml |
| Immunogen/Standard | Expression system for standard: E.coli; Immunogen sequence: P29-M212 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IL6 (Source: Uniprot.org, NCBI)
| Uniprot Id | P05231 |
|---|---|
| NCBI Gene Id | 3569 |
| Species Of This Entry | Human |
| Gene Name | IL6 |
| Protein Name | Interleukin-6 |
| Superfamily | IL-6 superfamily |
| Alternative Names | IL-6|B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A |
| Molecular Weight | 23718 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Human IL-6/Interleukin-6 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0410-CAP | Anti-Human IL6 Pre-coated 96-well strip microplate | 1 |
| EK0410-ST | Human IL6 Standard | 2 vials, 10 ng/tube |
| EK0410-DA | Human IL6 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0410 Human IL-6/Interleukin-6 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 4.69 | 9.38 | 18.75 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.031 | 0.091 | 0.127 | 0.222 | 0.513 | 0.749 | 1.186 | 1.997 |
We measured random samples of Human IL-6/Interleukin-6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 10 | 38 | 145 | 9 | 37 | 130 |
| Standard deviation | 0.62 | 1.67 | 10.73 | 0.64 | 1.66 | 11.18 |
| CV (%) | 6.2% | 4.4% | 7.4% | 7.2% | 4.5% | 8.6% |
We measured the performance consistency of Human IL-6/Interleukin-6 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 12 | 11 | 10 | 10 | 0.82 | 8.2% |
| Sample 2 | 38 | 43 | 40 | 42 | 40 | 1.92 | 4.8% |
| Sample 3 | 145 | 149 | 145 | 150 | 147 | 2.27 | 1.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 191
| Tutuncu EO,Dundar ZD,Kilinc I,Tutuncu A,Kocak S,Girisgin AS.Prognostic Value of Immunosuppressive Acidic Protein and Oxidative Stress Status in Critically Ill Patients.Indian J Crit Care Med.2021 Apr;25(4):405-409.doi:10.5005/jp-journals-10071-23788.PMID:34045807;PMCID:PMC8138636. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA |
PubMed ID 34045807 |
| Stellavato,A.;Restaino,O.F.;Vassallo,V.;Cassese,E.;Finamore,R.;Ruosi,C.;Schiraldi,C.Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes.Pharmaceutics 2021,13,737.https://doi.org/10.3390/pharmaceutics13050737 Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:CHONDROCYTES, DILUTION:NA |
View Publication |
| Zhang X,Zhu M,Hong Z,Chen C.Co-culturing polarized M2 Thp-1-derived macrophages enhance stemness of lung adenocarcinoma A549 cells.Ann Transl Med.2021 Apr;9(8):709.doi:10.21037/atm-21-1256.PMID:33987407;PMCID:PMC8106048. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:A549 CELL, DILUTION:NA |
PubMed ID 33987407 |
| Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828 Species: Human,Mouse EK0410 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA |
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| Liu T,Yin C,Li Y,Gao F,Yu L,Wang Z,Wang Q.Effects of Transcutaneous Electrical Acupoint Stimulation on Postoperative Cognitive Decline in Elderly Patients: A Pilot Study.Clin Interv Aging.2021 May 3;16:757-765.doi:10.2147/CIA.S309082.PMID:33976542;PMCID:PMC8106456. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 33976542 |
| Erica Costantini,Bruna Sinjari,Katia Falasca,Marcella Reale,Sergio Caputi,Srinivas Jagarlapodii,Giovanna Murmura," Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast",Mediators of Inflammation,vol.2021,Article ID 5562340,9 pages,2021. https://doi.org/10.1155/2021/5562340 Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HGF CELL, DILUTION:NA |
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| Pınar Bürhan-Çavuşoğlu,Evin İşcan,Ayşim Güneş,Neşe Atabey,Tunç Alkın,Increased telomerase activity in major depressive disorder with melancholic features: Possible role of pro-inflammatory cytokines and the brain-derived neurotrophic factor,Brain,Behavior,& Immunity - Health,2021,100259,ISSN 2666-3546,https://doi.org/10.1016/j.bbih.2021.100259. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA |
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| Yang N,Wang H,Zhang R,Niu Z,Zheng S,Zhang Z.C/EBP β mediates the aberrant inflammatory response and cell cycle arrest in LPS-stimulated human renal tubular epithelial cells by regulating NF-κB pathway.Arch Med Res.2021 May 1:S0188-4409(21)00079-5.doi:10.1016/j.arcmed.2021.03.008.Epub ahead of print.PMID:33947580. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA |
PubMed ID 33947580 |
| Yi Y,Chai X,Zheng L,Zhang Y,Shen J,Hu B,Tao G.CRISPR-edited CART with GM-CSF knockout and auto secretion of IL6 and IL1 blockers in patients with hematologic malignancy.Cell Discov.2021 Apr 27;7(1):27.doi:10.1038/s41421-021-00255-4.PMID:33907185. Species: Human,Mouse EK0410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA |
PubMed ID 33907185 |
| Yang M, Wang LI. MALAT1 knockdown protects from bronchial/tracheal smooth muscle cell injury via regulation of microRNA-133a/ryanodine receptor 2 axis. J Biosci. 2021;46:28. PMID: 33753579. Species: Human,Rat EK0410 usage in article: APP:ELISA, SAMPLE:B/TSMCS, DILUTION:NA |
PubMed ID 33753579 |
| Du W, Wang L, Liao Z, Wang J. Circ_0085289 Alleviates the Progression of Periodontitis by Regulating let-7f-5p/SOCS6 Pathway. Inflammation. 2021 Mar 12. doi: 10.1007/s10753-021-01445-8. Epub ahead of print. PMID: 33710445. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA |
PubMed ID 33710445 |
| Li C, Liu M, Liu K, Li M, Liu Y, Li T, Wei Y, Long Y, He W, Shi X, Li Y, Zhang H. BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody. Biochem Biophys Res Commun. 2021 Mar 16;551:155-160. doi: 10.1016/j.bbrc.2021.02.128. Epub ahead of print. PMID: 33740622. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 33740622 |
| Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310. Species: Human,Mouse EK0410 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA |
View Publication |
| Huang C,Chen Y,Lai B,Chen YX,Xu CY,Liu YF.Overexpression of SP1 restores autophagy to alleviate acute renal injury induced by ischemia-reperfusion through the miR-205/PTEN/Akt pathway.J Inflamm (Lond).2021 Feb 5;18(1):7.doi:10.1186/s12950-021-00270-y.PMID Species: Human,Rat EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA |
PubMed ID 33546692 |
| Junde Hou,Yanxin Cheng,Hongfang Wei et al.Dexmedetomidine Attenuates Hemodynamic and Proinflammatory Responses During Craniotomy for Traumatic Brain Injury:A Randomized Controlled Trial Study,02 February 2021,PREPRINT (Version 1) available at Research Squ Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
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| - Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
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| Qinchao Chen,Meng Cao,Hanyi Ge,"Knockdown of MALAT1 Inhibits the Progression of Chronic Periodontitis via Targeting miR-769-5p/HIF3A Axis",BioMed Research International,vol.2021,Article ID 8899863,12 pages,2021.https://doi.org/10.1155/2021/8899863 Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA |
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| Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi: Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA |
PubMed ID 33467440 |
| Gao H,Guo Z. LncRNA XIST regulates atherosclerosis progression in ox-LDL-induced HUVECs. Open Med (Wars). 2021 Jan 8;16(1):117-127.doi:10.1515/med-2021-0200.PMID:33542956;PMCID: PMC7819546. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION:NA |
PubMed ID 33542956 |
| Wei W,Yao Y,Bi H,Xu W,Gao Y.Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21-5p.Biochem Biophys Res Commun.2021 Jan 8;540:1-7.doi:10.1016/j.bbrc.2020.12.018.Epub ahead of print.PMID:33429194. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HK-2 CELL, DILUTION:NA |
PubMed ID 33429194 |
| Shaat, R.M.,Elsaid, T.O.,EL Baz, A. et al. Do obese patients with primary knee osteoarthritis benefit from a single bout of moderate intensity aerobic exercise?.Egypt Rheumatol Rehabil 47,27 (2020).https://doi.org/10. 1186/s43166-020-00029-y Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
View Publication |
| Fu,C.,Xu,Q.,Tang,S.,Cao,Y.,Liu,C.,Wang,Y.,Qian,Y.,Shi,F.,Gui,J.,Fan,Q., & Ling,Y.(2020).The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury.American journal of translational research, 12(11), 7114–712 Species: Human,Mouse EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 33312354 |
| Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432. Species: New Zealand white Rabbit EK0410 usage in article: APP:ELISA, SAMPLE:MSCS, DILUTION:NA |
PubMed ID 32090432 |
| Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865 Species: Human, Mouse EK0410 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
PubMed ID 31786500 |
| Liao H,Zhang H,Xiang J,Chen G,Cao Z.The effect of the surgical microscope on the outcome of root scaling. Am J Transl Res. 2020 Nov 15;12(11):7199-7210.PMID: 33312360;PMCID:PMC7724328. Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA |
PubMed ID 33312360 |
| Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov Species: Human |
PubMed ID 33159796 |
| Xue M, Peng N, Zhu X, Zhang H. Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis. Biochem Biophys Res Commun. 2020 Nov 25:S0006-291X(20)32086-6. doi Species: Human |
PubMed ID 33248690 |
| Asila A, Wang Y, Miao D, Yang X, Kaisaer Y, Ma L, Lv G, Yang C. SNHG16/miR-485-5p/BMP7 axis modulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Gene Med. 2020 Nov 11:e3296. doi: 10.1002/jgm.3296. Epub ahead of print Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:HBMSCS, DILUTION:NA |
PubMed ID 33179372 |
| Yuan X, Zhang L, Huang Y, Liu D, Peng P, Liu S, Long G, Hu G, Sun W. Induction of interleukin-6 by irradiation and its role in epithelial mesenchymal transition and radioresistance of nasopharyngeal carcinoma cells. Head Neck. 2020 Nov 5. doi: 10.1002/hed Species: Human EK0410 usage in article: APP:ELISA, SAMPLE:NPC CELLS, DILUTION:NA |
PubMed ID 33150659 |
| Hu Q, Peng J, Jiang L, Li W, Su Q, Zhang J, Li H, Song M, Cheng B, Xia J, Wu T. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis. 2020 Oct 28;11(10):925. doi: 10.1 Species: Mouse EK0410 usage in article: APP:ELISA, SAMPLE:CAL 27 AND HSC6 CELL LINES, DILUTION:NA |
PubMed ID 33116117 |
| Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33. | PubMed ID 31882548 |
| Serum Interleukin-6 is Not Linked with Sleep-Quality, Restless Legs Syndrome, and Depression, But with 6-Month Survival in Hematological Malignancies. | PubMed ID 30765978 |
| Evaluation of second trimester amniotic fluid ADAMTS4, ADAMTS5, interleukin-6 and tumor necrosis factor-α levels in patients with gestational diabetes mellitus. | PubMed ID 30623540 |
| The Effects of Resveratrol on Inflammation and Oxidative Stress in a Rat Model of Chronic Obstructive Pulmonary Disease | PubMed ID 28895883 |
| Novel therapeutic approach by nicotine in experimental model of multiple sclerosis | PubMed ID 23696955 |
| Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury | PubMed ID 29188738 |
| Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis | PubMed ID 27152709 |
| Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome | PubMed ID 29062341 |
| From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026 | PubMed ID 29036520 |
| E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury | PubMed ID 28740545 |
| Malnutrition and inflammation in acute kidney injury due to earthquake-related crush syndrome | PubMed ID 20346168 |
| Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine | PubMed ID 29049953 |
| Establishment of a Rabbit Model of Chronic Obstructive Sleep Apnea and Application in Cardiovascular Consequences | PubMed ID 28218220 |
| Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes | PubMed ID 26239742 |
| Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis | PubMed ID 26673286 |
| The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis | PubMed ID 29900992 |
| Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial | PubMed ID 29887900 |
| Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis | PubMed ID 29805485 |
| Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients | PubMed ID 29404146 |
| Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice | PubMed ID 28977826 |
| Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression | PubMed ID 28928376 |
| Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells | PubMed ID 28498464 |
| Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery | PubMed ID 28450928 |
| A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1?-induced activation of AP-1 in human OA chondrocytes | PubMed ID 28256567 |
| Placental Growth Factor, Soluble fms-Like Tyrosine Kinase 1, Soluble Endoglin, IL-6, and IL-16 as Biomarkers in Preeclampsia | PubMed ID 27799724 |
| Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage | PubMed ID 27698749 |
| Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells | PubMed ID 27679980 |
| Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial | PubMed ID 27651815 |
| Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis | PubMed ID 27602373 |
| LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling | PubMed ID 27590510 |
| Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease | PubMed ID 27445436 |
| miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40 | PubMed ID 27412561 |
| NF-?B-Regulated miR-99a Modulates Endothelial Cell Inflammation. | PubMed ID 27403035 |
| Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1? signaling pathway | PubMed ID 27398311 |
| Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome | PubMed ID 27168826 |
| Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1?, IL6, TNF?, and CRP) | PubMed ID 27127347 |
| Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes | PubMed ID 27082319 |
| GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-?B Pathway | PubMed ID 27057094 |
| Klotho Contributes to Pravastatin Effect on Suppressing IL-6 Production in Endothelial Cells | PubMed ID 27034587 |
| C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells | PubMed ID 27011164 |
| Salivary levels of interleukin-6 and tumor necrosis factor-? in patients undergoing hemodialysis | PubMed ID 26962319 |
| Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide | PubMed ID 26949603 |
| Association of serum immunoglobulin-G to Porphyromonas gingivalis with acute cerebral infarction in the Chinese population | PubMed ID 26941512 |
| Bclaf1 is an important NF-?B signaling transducer and C/EBP? regulator in DNA damage-induced senescence | PubMed ID 26794446 |
| IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel | PubMed ID 26503059 |
| miR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes | PubMed ID 26450708 |
| Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages | PubMed ID 25861639 |
| Flurbiprofen axetil reduces postoperative sufentanil consumption and enhances postoperative analgesic effects in patients with colorectal cancer surgery | PubMed ID 25663985 |
| TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells | PubMed ID 25360740 |
| Transcriptional Profiling Reveals Crosstalk Between Mesenchymal Stem Cells and Endothelial Cells Promoting Prevascularization by Reciprocal Mechanisms | PubMed ID 25299975 |
| RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms | PubMed ID 25254379 |
| A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-? activity | PubMed ID 25017038 |
| Evaluation of cytokines as Th1/Th2 markers in pathogenesis of children with Crimean-Congo hemorrhagic fever | PubMed ID 24753773 |
| Microvesicles secreted from human multiple myeloma cells promote angiogenesis | PubMed ID 24374814 |
| Effects of Pantoprazole on Systemic and Gastric Pro- and Anti-inflammatory Cytokines in Critically Ill Patients | PubMed ID 24250536 |
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Questions and answers from customers related to EK0410 Human IL-6/Interleukin-6 ELISA Kit PicoKine™
18 Related Questions
Question
Hello Support: I purchased Human IL-6 ELISA Kit but I have some questions, could you please give advice on staining technique? Is it possible to use only few wells for analysis of several samples and save other plates for further studies? Lastly, do I need to buy any software for data analysis, or Excel will be enough?
Verified Customer
Asked: 2020-04-30
Answer
The Human IL-6 ELISA Kit comes with 96 well with removable strips, so you can remove as many strips as you need before using it, then store the remaining strips in the pouch with desiccant, sealed it tightly and put in storage at 4C. For your data analysis excel will do. All you need to do is to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve.
Boster Scientific Support
Answered: 2020-04-30
Question
I purchased Human IL-6 ELISA Kit and I reconstitute with acetic acid and the coating buffer is alkaline solution of pH 9.6. I am concern with the pH change and possibly affects my results? Please let me know
Verified Customer
Asked: 2019-08-30
Answer
We recommend to use for coating buffers, the standard PBS rather than alkaline carbonate buffers for this antibody Human IL-6 ELISA Kit
Boster Scientific Support
Answered: 2019-08-30
Question
Is the Human IL-6 ELISA Kit have been tested with saliva samples? Do you have any suggestions?
Verified Customer
Asked: 2019-08-15
Answer
The Human IL-6 ELISA Kit is suitable to use in saliva samples and the usual protocol would apply. No need for pre-treatment of sample before assay.
Boster Scientific Support
Answered: 2019-08-15
Question
Please let me know if you CAN supply QC materials for EACH of these kits?. If you could introduce in EACH kit at least 2 or 3 vials with QC material (having 2 or 3 different concentrations of the analyte for which the kits are used) it would be PERFECT! (even if the raw materials of these QC vials will be the same as the standards
Verified Customer
Asked: 2019-02-12
Answer
We can not provide 2 or 3 different concentrations of the analyte(e.g. serum) for those kits. The QC vials we can provide are recombinant proteins of different concentrtaions.
Boster Scientific Support
Answered: 2019-02-12
Question
What are the minimum amounts of blood that must be collected to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF? I'd like to know the necessary amounts for each analysis done singly. Keyword: protocol, applications
Verified Customer
Asked: 2017-09-13
Answer
0.1ml sample will be added into each well, so you need to collect 0.6ml of sample at most if you plan to perform the analysis of ICAM, VCAM, IL-6, IL-10, IL-8 and TNF. And the sample needs to be diluted for the analysis of ICAM, VCAM.
Boster Scientific Support
Answered: 2017-09-13
Question
We received an inquiry regarding the EK0410 IL-6 ELISA Kit (Human) from a customer:
Verified Customer
Asked: 2016-01-06
Answer
<0.3pg/ml *The sensitivity or the minimum detectable dose (MDD) is the lower limit of target protein that can be detected by the kit. It is determined by adding two standard deviations to the mean O.D. value of twenty (20) blank wells and calculating the corresponding concentration.
Boster Scientific Support
Answered: 2016-01-06
Question
Q: can I use heparin plasma as samples in Human IL-6 Picokine® ELISA Kit (Catalog # EK0410)?
Verified Customer
Asked: 2020-03-20
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-6 causing disruption of its protein structure. IL-6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-6 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2019-11-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-22
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2019-07-04
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-04
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-6 using the EK0410 Human IL-6 Picokine® ELISA Kit?
E. Puri
Asked: 2019-03-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Harris
Asked: 2017-07-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-07-23
Question
Q: how to proceed with the analysis of ELISA data? I have obtained IL-6 level in plasma.
Verified Customer
Asked: 2016-07-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-29
Question
What is the expiration date of EK0410 from the time of manufacturing, 6 months or 1 year?
Asked: 2019-12-17
Answer
Expiration is based on storage temperature. Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Boster Scientific Support
Answered: 2019-12-17
Question
Is it normal to have fluid in the well of the EK0410 plate and a solid standard that is very hard to dissolve.
Asked: 2019-04-01
Answer
For the Human IL-6 ELISA Kit PicoKine™ (EK0410), it is normal that there is fluid in the wells of the plate, and the drops could be removed by patting the plate on a paper towel or by aspiration. For the standard, we never encountered this problem before, and our technicians suggests to centrifuge at 2000-4000×g for 5 minutes after dissolving the standard.
Boster Scientific Support
Answered: 2019-04-02
Question
Have you tested EK0410 in tissue homogenates and Cell culture supernates?
Asked: 2019-03-15
Answer
The Human IL-6 EK0410 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
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