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Quantitative Real-time PCR (qPCR) is the gold standard for the precise nucleic acids quantification in genomics research, biologics testing and drug discovery. Our qPCR experts are excited to accelerate your research with Boster’s Fast, sensitive, and reliable service.
Identification of tumor cell lines for drug efficacy
Accurate copy number variation analysis
Predicting a drug's response in animal or cellular models
Analysis of time or dose dependency and mode of action
Stratification marker verification and allelic discrimination
Pathogen detection and quantification
from sample prep to data analysis
Assay Development Experts
qPCR primer design, optimization, and troubleshooting
High Throughput Capacity
qPCR solutions to rapidly scale pilot or exploratory projects
Complete CRO Service
qPCR combined with Boster's other services for a complete CRO service
As easy as 1, 2, 3.
|Total RNA||RNA isolated from >1 million cells supplied in 1 ml TRIzol solution, please ship on dry ice|
|Cell Pellet||Please prepare from >1 million mammalian cells, please ship on dry ice|
|cDNA||2ug of cDNA for each gene to be tested per sample (in TE buffer, at least 100 ng/ul)|
|Primers/Probes||100 ul of primers/probes at 100uM per target gene|
It is our project concierges' mission to make your project experience as smooth and memorable as possible. They are subject matter experts who are easily accessible around the clock, always happy to help you solve problems, make recommendations and sort through options.
Real-Time PCR (RT-PCR) or Quantitative PCR (qPCR) is a sensitive and reproducible method of determining the levels of gene expression in an organism. Simply put it is a way of finding out how much of a specific section of DNA is in a sample, in Real Time. Widely known as the “Gold Standard” for DNA and RNA quantification and genotyping, it can be used in the drug discovery process to quantify changes in mRNA expression of drug targets, PD markers or predictive biomarkers.
qPCR builds on the principal of PCR where amplification is achieved using nucleotides and primers, with fluorescent dyes or DNA probes added to detect PCR products as they are produced. Specific nucleic acids can be quantified by Absolute Quantification, by comparing the sample to a DNA standard to measure the exact number of target DNA molecules, or Relative Quantification using internal reference genes within the sample to determine the quantity of the target gene.
qPCR provides fast and high-throughput detection and quantification of target DNA sequences by lowering amplification and allowing simultaneous visualization of all newly formed DNA amplicons.
Boster Bio will test your samples and provide you scientific consultation, backed by our network of experts with experience in your analytes.
Boster offers competitively priced Western blot services with quick turnaround. We also provide 2D-WB and LC-MS for advanced characterization.
Boster offers competitively priced histology/IHC/IF services with quick turnaround. Featuring a large selection of FFPE blocks and tissue/cell arrays.
Ready-to-go screening assays to ensure your discovery process stays on track from the earliest discovery stages.
Get fully validated, affinity matured rabbit monoclonal antibodies using our patented microfluidics PCD platform.
Boster Bio offers a full range of quality NGS services extending from nucleic acid extraction, to library preparation, sequencing and bioinformatic analysis.