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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
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The first step of gel preparation is to determine the gel percentage based on the molecular weight of your proteinsample:
If you are not sure of the size of your protein or are looking at proteins of a variety of molecular weights, then a gradient gel may provide the best resolution.
After electrophoresis, we recommend using one of our gel staining solutions to determine if the electrophoretic separation works. Refer to the datasheet(s) on our website for more information.
Note: Stained gel cannot be used in the subsequent protein transfer procedure.
After blocking, the membrane is incubated with a primary antibody (that binds to the target protein)followed by a HRP- or AP-conjugated secondary antibody.
In this section, we provide the protocols for the Enhanced Chemiluminescence Detection (ECL) and colorimetric detection (DAB) methods. Use the method that fits your preferences and criteria.
* Each kit has sufficient reagents for 800 cm2 of membrane.
†Instead of using the ECL kit which provides 1) chromogenic reagents A and B (20X concentrated; 5 mL), 2) blocking buffer and 3) HRP-conjugated secondary antibody, one may use one of the following standalone chromogenic reagents A and B from Boster:
Develop and fix the film in a dark room immediately using our recommended WB Developing and Fixing Kit (Boster Catalog # AR0132); Alternatively, fluorescence CCD scan, digital imager or luminometer can be used).
Prepare DAB or BCIP/NBT substrate solution described below.