IHC Microscopy

IHC Resource Center

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IHC Technical Resources

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IHC Principle

Western blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.

IHC is the application of the immunoassay principle to tissue staining. By using antibodies to direct staining, IHC can accurately detect protein localization while generating crisp stains of tissue structures.


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