Custom Polyclonal Antibody Production Service
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>Service Packages Pricing
>Project Timeline
>Boster Guarantee
>Service Details
>Additional Services
>About Boster Biological Technology
Service Packages Pricing
Package 1
$800
User Provide Antigen
The most economical solution on the market for custom polyclonal antibody using user provided antigen. Basic screening via direct ELISA.
Dedicated project manager - Weekly updates on project status
Antigen synthesis - Immunizing 2 rabbits
- antibody purification
- Shipping and handling
-
Deliverables:
- Internal test result images will be sent to the customer
- A small sample will be sent to be validated by customer after we have tested it on WB and IHC internally internal testing.
- 1mg of the purified antibody
- VIP status at Bosterbio.com
1mg of blocking peptide or 50ng of recombinant protein
Package 2
$1300
Boster Provide Antigen
Do not have an antigen? No worries. We will synthesize an antigen for you for only $500.
- Dedicated project manager
- Weekly updates on project status
- Antigen synthesis
- Immunizing 2 rabbits
- Antigen Affinity Purification
- Shipping and handling
-
Deliverables:
- Internal test result images will be sent to the customer
- A small sample will be sent to be validated by customer after we have tested it on WB and IHC internally internal testing.
- 1mg of the purified antibody
- 1mg of blocking peptide or 50ng of recombinant protein
- VIP status at Bosterbio.com
Payment information/options
The way that payment works is that you will be invoiced half the cost when we start the gene syntheses for the immunogen. By default, antibody success is dependent on the reaction of the antigen that the user provides on the direct ELISA assay. The other half will be invoiced upon success. If for any reason you do not think the antibody generated successfully fulfills the success criteria, then please let us know. If we agree that this project is unsuccessful, then other half will not be invoiced.
If the project is not successful, if you wish, then we are able to make a second attempt for half of the cost of the project.
Downstream Application Insurance
As an additional risk mitigation option, users can specify a few downstream applications as the success criteria. Any additional criteria needs to be mentioned in the beginning of the project. Please note that this option would cost extra based on the downstream applications chosen.
If the antibody does not work for the requested downstream appliaction, then the customer will pay a reduce priced for the project.
Service Details
| Phase | Description/Timeline |
|---|---|
| Phase I Antigen Preparation |
Package 1: Customer Provide Antigen Timeline: 1 week for delivery. Requirements: The concentration of the antigen should be at least 0.5 mg/mL and the amount needed is at least 5mg. For the synthetic polypeptide antigen, please provide conjugated peptides (12-16aa) and LC/MS data. And the purity is at least 90%. For the recombinant protein antigen, please provide SDS-PAGE analysis report. And the purity is at least 85%. Also, tell us your desired end application (Optional). Package 2: Boster provides antigen Timeline: 4 weeks Requirements: Immunogen Sequence and your desired end application (Optional) We can produce synthetic polypeptide antigen and recombinant protein antigen. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us. |
| Phase II Animal immunization |
Timeline: 10 weeks. Conjugated peptides or recombinant proteins are used to immunize 2 rabbits.
|
| Phase III Antigen Affinity Purification and Validation |
Timeline: 1-2 weeks Package 1: If more than 1mg antigen is provided by the customer, we can do affinity purification at not additional costs. You have the option of protein A, protein G ,or ion-exchange. Package 2: Antigen Affinity Purification: Antibody will be validated on Western Blot and IHC-P (if applicable) Validation : If the antibody reacts to the direct ELISA, then the project will be considered successful. |
| Deliverables |
Package 1 Package 2 |
Boster Guarantee
Expertise
Team of experts with decades of combined experience dedicated to producing your polyclonal antibody.
Communication
A dedicated point of contact for you throughout the polyclonal project. Weekly project updates available.
Validation
Basic validation via direct ELISA. Advanced application-specific validation available upon request.
Additional Services
Antigen Synthesis
Contact us for pricing
Instead of providing us the immunogen, we can produce synthetic polypeptide antigen and recombinant protein antigen for your target of interest.This service takes 1 additional month.
Custom Screening Criteria
Contact us for pricing
We can conduct additional screening methods per your requirement. Such as testing anti/sera and or culture supernatants in chosen samples on WB, IHC, Flow etc.
Blocking Peptide
Contact us for pricing
If you require a blocking peptide for your developed antibody, we can help you with that.
About Boster Biological Technology
1993
Serving scientists ever since
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