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In need of a custom antibody? Work with the Antibody Experts--Boster Bio has been making antibodies since 1993 and cited by 30,000+ publications for antibody based applications.See contract details
Our 25+ years of experience in custom antibody design and production will ensure you the best chance of getting a high affinity custom polyclonal antibody that works for your application. Since 1993, our team has produced over 10,000 rabbit polyclonal antibodies. All you need to provide is your target sequence and Boster gets to work.
Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to drop us an email at [email protected] and we’ll make sure you stay informed.
We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modifcation, secondary/tertiary structure and epitope localization.
We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.
Standard package starting from $1300. Additional services cost see section "ADDITIONAL SERVICES".
Recombinant antigen design
Immunization of 2 rabbits
Affinity purification and validation
3-5mg antibody, ELISA test report
Below is a general timeline of a custom polyclonal antibody project. For any questions, please contact [email protected]
|Pre-immune sera collection (optional)||Day 0||(Optional) Pre-immune bleed 0.5ml per rabbit.|
|Plasmid synthesis||Day 1||Synthesizing your antigen expression plasmid.|
|Recombinant antigen expression||Day 14||Express your protein antigen recombinantly.||(Optional) Pre-immune screening report|
|Immunization preparation||Day 24 (Day 1 if you provide antigen)||Preparing antigen with CFA or IFA, preparing rabbits.|
|Immunization 1st shot||Day 31||2 New Zealand White Rabbits, 8-10 subcutaneous immune boost injections, 0.5mg of antigen total per rabbit.|
|Immunization 2nd shot||Day 52||6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.|
|Immunization 3rd shot||Day 66||6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.|
|Immunization 4th shot||Day 81||6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.|
|Sera collection, purification and ELISA test||Day 95||Purify the anti-sera with antigen affinity chromatography, test the antibody via ELISA, decide to continue or terminate project based on the ELISA result and (optional) specific application test result.||(Optional) A sample vial of antibody of custom amount for specific application validation.|
|Prepare antibody for shipping||Day 102||Prepare antibodies for shipping, which may take 10-15 days. Deliver the final report to customer online.||
*Why do I want pre-immune sera? Sometimes antibody immunization fails because some hosts have existing immunity towards your target protein of interest. This is especially common if the protein originated from organisms is related to common pathogens. Screening the hosts’ sera against your protein of interest can help mitigate this risk.
See what scientists say about our custom antibodies
Good Zebrafish Antibody For Nlz2
Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.
Immunofluorescence, Zebrafish embryos 24hpf, Confocal Microscopy
A Good Drosophila Me31B Antibody For WB
The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service.
Western Blot, Insect, D. melanogaster, ECL
Good Zebrafish CDHR1a Antibody From Boster Bio
The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!
Immunofluorescence, Zebrafish embryos, 5 days old, Confocal Microscopy
Verification Of Antibody On Recombinant Protein
We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.
Western Blot, Recombinant protein expressed in and purified from E. coli, Chemiluminescence
Feel free to contact us to discuss details about your custom antibody project.