Custom Polyclonal Antibody
Production Service

Expert Design for High-Affinity Custom Rabbit Polyclonal Antibodies

Why Use Boster for your custom antibody projects

Because we are one of the largest antibody manufacturer with 3 decades of expertise

Our 25+ years of experience in antibody design and production will ensure you the best chance of getting a high affinity polyclonal antibody that works for your application. Since 1993, our team has produced over 10,000 rabbit polyclonal antibodies. All you need to provide is your target sequence and Boster gets to work.

Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to drop us an email at [email protected] and we’ll make sure you stay informed.

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What Makes A Good Antibody?

Boster Antigen design and expression overview

Design

We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modifcation, secondary/tertiary structure and epitope localization.

Expression

We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.

Custom Antibody Service Overview

Standard package starting from $1300. Additional services cost see section "ADDITIONAL SERVICES".

Antigen

Recombinant antigen design

Immunization

Immunization of 2 rabbits

Purification

Affinity purification and validation

Deliveries

3-5mg antibody, ELISA test report

Project Timeline

Below is a general timeline of a custom polyclonal antibody project. For any questions, please contact [email protected]

Milestone	Start Day	Description	Deliverables
Pre-immune sera collection (optional)	Day 0	(Optional) Pre-immune bleed 0.5ml per rabbit.
Plasmid synthesis	Day 1	Synthesizing your antigen expression plasmid.
Recombinant antigen expression	Day 14	Express your protein antigen recombinantly.	(Optional) Pre-immune screening report
Immunization preparation	Day 24 (Day 1 if you provide antigen)	Preparing antigen with CFA or IFA, preparing rabbits.
Immunization 1st shot	Day 31	2 New Zealand White Rabbits, 8-10 subcutaneous immune boost injections, 0.5mg of antigen total per rabbit.
Immunization 2nd shot	Day 52	6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Immunization 3rd shot	Day 66	6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Immunization 4th shot	Day 81	6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Sera collection, purification and ELISA test	Day 95	Purify the anti-sera with antigen affinity chromatography, test the antibody via ELISA, decide to continue or terminate project based on the ELISA result and (optional) specific application test result.	(Optional) A sample vial of antibody of custom amount for specific application validation.
Prepare antibody for shipping	Day 102	Prepare antibodies for shipping, which may take 10-15 days. Deliver the final report to customer online.	ELISA test report, 3-5mg of the purified antibody. *Guaranteed to have at least 3mg and at most 5mg. Additional antibody can be purchased.

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Additional Services

Pre-immune screening

Order a vial of pre-immune serum for screening existing immunity against the target protein, and for control purposes.*
Price: $100 per rabbit, 1ml of serum, not purified

Interim test bleeds

Order a vial of test serum after each of the immune booster shots to verify immune response’s antigen specificity and Ig isotypes.
Price: $50 per rabbit per bleed, 1ml of anti-serum, not purified

Additional rabbits

Order additional rabbits to improve the yield and success rate of your project. If the rabbit is positive and passes QC, 2mg of antibody will be added to your final deliverable.
Price: $200 per rabbit

Additional antibodies

If there are remaining antibodies from the batch, additional antibodies are available for purchase.
Price:
$150 for additional 100ug
$500 for additional 1mg
$1000 for additional 10mg (if available)

Blocking Peptide

If you require a blocking peptide for your developed antibody, we can help you with that.
Price: $150 for 1mg of blocking peptide

Recombinant Protein

If you want the recombinant protein we generated for you, you can purchase it from us.
Price: $200 for 1mg of purified recombinant protein. Inquire for bulk pricing.

Specific Application Insurance

Purchase an insurance for your antibody to work for certain applications.
Price: Custom, depending on the application and nature of the target protein

Intellectual Property & Exclusivity

If you intend to have exclusive rights for the produced antibodies, we can create a license that fits your needs.
Price: Custom, depending on the intended use of the antibody

*Why do I want pre-immune sera? Sometimes antibody immunization fails because some hosts have existing immunity towards your target protein of interest. This is especially common if the protein originated from organisms is related to common pathogens. Screening the hosts’ sera against your protein of interest can help mitigate this risk.

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Jakub Famulski

Principal Investigator

Good Zebrafish Antibody For Nlz2

Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.

Immunofluorescence, Zebrafish embryos 24hpf, Confocal Microscopy

Svetlana Dzitoyeva

Research Scientist

A Good Drosophila Me31B Antibody For WB

The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service.

Western Blot, Insect, D. melanogaster, ECL

Jakub Famulski

Principal Investigator

Good Zebrafish CDHR1a Antibody From Boster Bio

The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!

Immunofluorescence, Zebrafish embryos, 5 days old, Confocal Microscopy

Hanjo Hellmann

Associate Professor

Verification Of Antibody On Recombinant Protein

We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.

Western Blot, Recombinant protein expressed in and purified from E. coli, Chemiluminescence