Custom Polyclonal Antibody

Svetlana Dzitoyeva

Research Scientist

A Good Drosophila Me31B Antibody For WB

The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service.

Western Blot, Insect, D. melanogaster, ECL

Jakub Famulski

Principal Investigator

Good Zebrafish CDHR1a Antibody From Boster Bio

The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!

Immunofluorescence, Zebrafish embryos, 5 days old, Confocal Microscopy

Hanjo Hellmann

Associate Professor

Verification Of Antibody On Recombinant Protein

We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.

Western Blot, Recombinant protein expressed in and purified from E. coli, Chemiluminescence