In need of a custom antibody? Work with the Antibody Experts--Boster Bio has been making antibodies since 1993 and cited by 30,000+ publications for antibody based applications.
Because we are one of the largest antibody manufacturer with 3 decades of expertise
Our 30+ years of experience in custom antibody design and production will ensure you the best chance of getting a high affinity custom polyclonal antibody that works for your application. Since 1993, our team has produced over 10,000 rabbit
polyclonal antibodies. All you need to provide is your target sequence and Boster gets to work.
Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the
way, feel free to drop us an email at [email protected] and we’ll make sure you stay informed.
We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modification, secondary/tertiary structure and
epitope localization.
Expression
We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.
Available Hosts
Although this is a custom rabbit polyclonal service, alternative hosts such as guinea pig, chicken, and goat are available if needed for your project. Please inquire.
Rabbit
Guinea Pig
Chicken
Goat
Custom Antibody Service Overview
Standard package starting from $1300. Additional services cost see section "ADDITIONAL SERVICES".
Antigen
Recombinant antigen design
Immunization
Immunization of 2 rabbits
Purification
Affinity purification and validation
Deliveries
3-5mg antibody, ELISA test report
Project Timeline
Below is a general timeline of a custom polyclonal antibody project. For any questions, please contact [email protected]
Milestone
Start Day
Description
Deliverables
Pre-immune sera collection (optional)
Day 0
(Optional) Pre-immune bleed 0.5ml per rabbit.
Plasmid synthesis
Day 1
Synthesizing your antigen expression plasmid.
Recombinant antigen expression
Day 14
Express your protein antigen recombinantly.
(Optional) Pre-immune screening report
Immunization preparation
Day 24 (Day 1 if you provide antigen)
Preparing antigen with CFA or IFA, preparing rabbits.
Immunization 1st shot
Day 31
2 New Zealand White Rabbits, 8-10 subcutaneous immune boost injections, 0.5mg of antigen total per rabbit.
Immunization 2nd shot
Day 52
6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Immunization 3rd shot
Day 66
6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Immunization 4th shot
Day 81
6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit.
Sera collection, purification and ELISA test
Day 95
Purify the anti-sera with antigen affinity chromatography, test the antibody via ELISA, decide to continue or terminate project based on the ELISA result and (optional) specific application test result.
(Optional) A sample vial of antibody of custom amount for specific application validation.
Prepare antibody for shipping
Day 102
Prepare antibodies for shipping, which may take 10-15 days. Deliver the final report to customer online.
ELISA test report, 3-5mg of the purified antibody.
*Guaranteed to have at least 3mg and at most 5mg. Additional antibody can be purchased.
Order additional rabbits to improve the yield and success rate of your project. If the rabbit is positive and passes QC, 2mg of antibody will be added to your final deliverable.
If you intend to have exclusive rights for the produced antibodies, we can create a license that fits your needs.
Price: Custom, depending on the intended use of the antibody
*Why do I want pre-immune sera? Sometimes antibody immunization fails because some hosts have existing immunity towards your target protein of interest. This is especially common if the protein originated from organisms is related to
common
pathogens. Screening the hosts’ sera against your protein of interest can help mitigate this risk.
See what scientists say about our custom antibodies
Jakub Famulski
Principal Investigator, Department of Biology, University of Kentucky
Good Zebrafish Antibody for Nlz2
Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.
Research Scientist, Biochemistry and Molecular Genetics, University of Colorado, Denver
A Good Drosophila Me31B Antibody For WB
The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service.
Western Blot, Insect, D. melanogaster, ECL, Catalog# DZ33938-1
Erik de Vrieze
Postdoctoral Researcher, Dept of Otorhinolaryngology & Dept of Human Genetics, Radboud University Medical Center
Thank you for all the work on this project
First, we received our shipment of antibodies in perfect condition. Thank you for all the work on this project. On the left, you can find pictures of 5dpf wildtype and Ush2a mutant zebrafish retina stained with DZ33954 in red. The pictures show the typical layered retinal structure, with punctate Ush2a staining at the connecting cilium (cc). In blue, the nuclei of inner and outer nuclear layers are shown (INL/ONL).
Principal Investigator, Department of Biology, University of Kentucky
Good Zebrafish CDHR1a Antibody From Boster Bio
The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!
Immunofluorescence, Zebrafish embryos, 5 days old, Confocal Microscopy, Catalog# DZ07988
Jingli Cao
Assistant Professor, Weill Cornell Medical College
Good Zebrafish Mef2 Antibody
Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature.
Principal Investigator, Department of Biology, University of Kentucky
Good Zebrafish-Specific Siah1 Antibody From Boster Bio
Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!
Associate Professor, School of Biological Sciences, Washington State University
Verification Of Antibody On Recombinant Protein
We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.
Western Blot, Recombinant protein expressed in and purified from E. coli, Chemiluminescence, Catalog# DZ33980
Byron Baron
Principal Investigator & Research Academic and Lecturer, Centre for Molecular Medicine and Biobanking, Faculty of Medicine and Surgery, University of Malta
It worked relatively well
We have no alternative and it worked relatively well. The stability at room temperature is impressive considering the antibody took 3 weeks to be delivered.
Western blot, human and mouse whole cell lysates, Catalog# DZ41142
Yuzuru Imai
Associate Professor, Department of Research for Parkinson's Disease, Juntendo University Graduate School of Medicine
Western blotting is sufficient with this antibody
There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody.
Western blot, Drosophila melanogaster (head and thorax), Catalog# DZ41152
Amin Ghabrial
Principal Investigator, Pathology and Cell Biology, Columbia University
The only antibody to Drosophila archease available and it works
My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining. Antibody staining reveals subcellular localization of the protein. In the salivary gland and trachea, localization was primarily nuclear. Polyclonal sera works with little background. May have to optimize staining. The bottom line: The only antibody to Drosophila archease available and it works.
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Boster Bio Anti-Photomedin-1 Antibody catalog # A15100. Tested in WB applications. This antibody reacts with Human. Supplied as 100μl in Liquid form antibody.
Looking to outsource your ELISA experiments? Do it with Boster, one of the biggest ELISA manufacturers. We provide research grade CRO services focusing on academic research and publishing.