Custom Polyclonal Antibody Production Service

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Service Packages Pricing

Package 1

$800

User Provides Antigen

The most economical solution on the market for custom polyclonal antibody using user-provided antigen. Basic screening via direct ELISA.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 2 rabbits
  • Antibody purification
  • Shipping and handling
  • Deliverables:
  • Internal test result images will be sent to the customer
  • A small sample will be sent to be validated by the customer after we have internally tested it on WB and IHC.
  • 1mg of the purified antibody
  • 1mg of blocking peptide or 1mg of recombinant protein
  • VIP status at Bosterbio.com

Package 2

$1300

Boster Provides Antigen

Do not have an antigen? No worries. We will synthesize an antigen for you for only $500.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 2 rabbits
  • Antigen affinity purification
  • Shipping and handling
  • Deliverables:
  • Internal test result images will be sent to the customer
  • A small sample will be sent to be validated by the customer after we have internally tested it on WB and IHC.
  • 1mg of the purified antibody
  • 1mg of blocking peptide or 1mg of recombinant protein
  • VIP status at Bosterbio.com


Payment Information/Options

You will be invoiced half the cost when we start the gene syntheses for the immunogen. By default, antibody success is dependent on the reaction of the antigen that the user provides for the direct ELISA. The other half will be invoiced upon success. If for any reason you do not think the antibody generated fulfills the success criteria, please let us know. If we agree that the project is unsuccessful, the other half of the cost will not be invoiced.

If the project is not successful and you would like to try again, we are able to make a second attempt for half the cost of the project.


Downstream Application Insurance

As an additional risk mitigation option, users can specify a few downstream applications as the success criteria. Any additional criteria needs to be mentioned at the beginning of the project. Please note that this option would cost extra depending on the downstream applications chosen.
If the antibody does not work for the requested downstream application, then the customer will pay a reduced price for the project.

We respect our customer's confidential information and guarantee that all intellectual property remains with the customer.

Milestone Duration
Plasmid Synthesis 14 days
Antigen Expression 10 days
Immunization First Shot 3 weeks + 7 days

3 weeks: The time interval between the first immunization and the second immunization

7 days: Shipping and pre-immunization preparation work
Immunization Second Shot 2 weeks
Immunization Third Shot 2 weeks
Immunization Fourth Shot 2 weeks + 5 days

*The extra 5 days is for bleeding and shipping
Serum Purification And Testing 1 week
Deliver Reports 1 day

Deliverables: WB, IHC test report, 1mg of the purified antibody, and 1mg of purified recombinant protein

*Shipping may take 7-10 business days

Service Details

Phase Description/Timeline
Phase I

Antigen Preparation
Package 1: Customer Provides Antigen
Timeline: 1 week for delivery
Requirements: The concentration of the antigen should be at least 0.5 mg/mL and the amount needed is at least 5mg. For the synthetic polypeptide antigen, please provide conjugated peptides (12-22aa) and LC/MS data. The purity should be at least 90%. For the recombinant protein antigen, please provide SDS-PAGE analysis report. The purity should be at least 85%. Also, please tell us your desired end application (optional).

Package 2: Boster provides antigen
Timeline: 4 weeks
Requirements: Immunogen sequence and your desired end application (optional)

We can produce synthetic polypeptide antigen and recombinant protein antigen. If you have questions about which one is better for your particular needs or you want to use other types of antigen, please contact us.
Phase II

Animal Immunization
Timeline: 10 weeks
Conjugated peptides or recombinant proteins are used to immunize 2 rabbits.
  • Week 0: Bleed 3-5 ml (yields 1-2 ml pre-immune serum). Immunize with 500 µg/rabbit antigen in CFA.
  • Week 3: Immunize with 250 µg antigen/rabbit in IFA.
  • Week 5: Immunize with 250 µg antigen/rabbit in IFA.
  • Week 6: Test bleed. Adjust the subsequent immunization schedule accordingly.
  • Week 7: Immunize with 250 µg antigen/rabbit in IFA.
  • Week 8: N/A
  • Week 9: Test bleed. If tests are negative, immunize with 250 µg antigen/rabbit in IFA.
  • Week 10: Terminating bleed 40-50 ml/rabbit. Test antibody purified from bleed 1-2 ml. ELISA, Western blotting (WB) and Immunohistochemistry (IHC) on appropriate cells/tissue.
Phase III

Antigen Affinity Purification and Validation
Timeline: 1 week

Package 1: If more than 1mg antigen is provided by the customer, we can do affinity purification at no additional costs. You have the option of protein A, protein G , or ion-exchange.

Package 2: Antigen Affinity Purification: Antibody will be validated on Western Blot and IHC-P (if applicable).
Validation: If the antibody reacts to the direct ELISA, then the project will be considered successful.
Deliverables

Package 1

  • Internal test result images will be sent to the customer.
  • A small sample will be sent to the customer to validate on their end after we have tested it on WB and IHC-P internally.
  • 1mg of the purified antibody

Package 2

  • Internal test result images will be sent to the customer
  • A small sample will be sent to the customer to validate on their end after we have tested it on WB and IHC internally
  • 1mg of the purified antibody
  • 1mg of blocking peptide or 1mg recombinant protein

Boster Guarantee

Expertise

Team of experts with decades of combined experience dedicated to producing your polyclonal antibody.

Communication

A dedicated point of contact for you throughout the polyclonal project. Weekly project updates available.

Validation

Basic validation via direct ELISA. Advanced application-specific validation available upon request.

Additional Services

Antigen Synthesis

Contact us for pricing

Instead of providing us the immunogen, we can produce synthetic polypeptide antigen and recombinant protein antigen for your target of interest. This service takes 1 additional month.

Custom Screening Criteria

Contact us for pricing

We can conduct additional screening methods per your requirement, such as testing anti/sera and or culture supernatants in chosen samples on WB, IHC, Flow, etc.

Blocking Peptide

Contact us for pricing

If you require a blocking peptide for your developed antibody, we can help you with that.

Pre-bleed Screening

Contact us for pricing

Get 5ml of pre-bleed for screening purposes prior to commencing with custom-based projects. If you are unfamiliar with this procedure, please inquire.

About Boster Biological Technology

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