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Antibody Production Service
In need of a custom antibody? Work with the Antibody Experts--Boster Bio has been making antibodies since 1993. As a leading provider of custom rabbit polyclonal antibody service, our work has been recognized and cited by 60,000+ publications for antibody based applications.
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Because we are one of the largest antibody manufacturer with 3 decades of expertise
Our 30+ years of experience in custom antibody design and production will ensure you the best chance of getting a high affinity custom polyclonal antibody that works for your application. Since 1993, our team has produced over 10,000 rabbit polyclonal antibodies. All you need to provide is your target sequence and Boster gets to work.
Our team of experts will provide support for each step of antibody production - from antigen design to final antibody purification. You will be notified when each milestone is achieved. If you have any questions along the way, feel free to drop us an email at [email protected] and we’ll make sure you stay informed.
Boster Antigen design and expression overview
Design
We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modification, secondary/tertiary structure and epitope localization.
Expression
We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.
Although this is a custom rabbit polyclonal service, alternative hosts such as guinea pig, chicken, and goat are available if needed for your project. Please inquire.
CUSTOM Rabbit Polyclonal Antibody Services OVERVIEW
Standard package starting from $1300. Additional services cost see section "ADDITIONAL SERVICES".
Antigen
Recombinant antigen design
Immunization
Immunization of 2 rabbits
Purification
Affinity purification and validation
Deliveries
3-5mg antibody, ELISA test report
Below is a general timeline of a custom polyclonal antibody project. For any questions, please contact [email protected]
Milestone | Start Day | Description | Deliverables |
---|---|---|---|
Pre-immune sera collection (optional) | Day 0 | (Optional) Pre-immune bleed 0.5ml per rabbit. | |
Plasmid synthesis | Day 1 | Synthesizing your antigen expression plasmid. | |
Recombinant antigen expression | Day 14 | Express your protein antigen recombinantly. | (Optional) Pre-immune screening report |
Immunization preparation | Day 24 (Day 1 if you provide antigen) | Preparing antigen with CFA or IFA, preparing rabbits. | |
Immunization 1st shot | Day 31 | 2 New Zealand White Rabbits, 8-10 subcutaneous immune boost injections, 0.5mg of antigen total per rabbit. | |
Immunization 2nd shot | Day 52 | 6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit. | |
Immunization 3rd shot | Day 66 | 6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit. | |
Immunization 4th shot | Day 81 | 6-8 subcutaneous immune boost injections, 0.25mg of antigen total per rabbit. | |
Sera collection, purification and ELISA test | Day 95 | Purify the anti-sera with antigen affinity chromatography, test the antibody via ELISA, decide to continue or terminate project based on the ELISA result and (optional) specific application test result. | (Optional) A sample vial of antibody of custom amount for specific application validation. |
Prepare antibody for shipping | Day 102 | Prepare antibodies for shipping, which may take 10-15 days. Deliver the final report to customer online. |
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Specific Application Insurance
Intellectual Property & Exclusivity
*Why do I want pre-immune sera? Sometimes antibody immunization fails because some hosts have existing immunity towards your target protein of interest. This is especially common if the protein originated from organisms is related to common pathogens. Screening the hosts’ sera against your protein of interest can help mitigate this risk.
Custom Antibody Production:
See what scientists say about our custom antibodies
Have Questions?
Feel free to contact us to discuss details about your custom antibody project.
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