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Custom Polyclonal Antibody Production Service
Table of Contents
>Service Packages Pricing
>Project Timeline
>Boster Guarantee
>Service Details
>Additional Services
>About Boster Biological Technology
Service Packages Pricing
Package 1
$800
User Provides Antigen
The most economical solution on the market for custom polyclonal antibody using user-provided antigen. Basic screening via direct ELISA.
Dedicated project manager - Weekly updates on project status
Antigen synthesis - Immunizing 2 rabbits
- Antibody purification
- Shipping and handling
-
Deliverables:
- Internal test result images will be sent to the customer
- A small sample will be sent to be validated by the customer after we have internally tested it on WB and IHC.
- 1mg of the purified antibody
- VIP status at Bosterbio.com
1mg of blocking peptide or 1mg of recombinant protein
Package 2
$1300
Boster Provides Antigen
Do not have an antigen? No worries. We will synthesize an antigen for you for only $500.
- Dedicated project manager
- Weekly updates on project status
- Antigen synthesis
- Immunizing 2 rabbits
- Antigen affinity purification
- Shipping and handling
-
Deliverables:
- Internal test result images will be sent to the customer
- A small sample will be sent to be validated by the customer after we have internally tested it on WB and IHC.
- 1mg of the purified antibody
- 1mg of blocking peptide or 1mg of recombinant protein
- VIP status at Bosterbio.com
Payment Information/Options
You will be invoiced half the cost when we start the gene syntheses for the immunogen. By default, antibody success is dependent on the reaction of the antigen that the user provides for the direct ELISA. The other half will be invoiced upon success. If for any reason you do not think the antibody generated fulfills the success criteria, please let us know. If we agree that the project is unsuccessful, the other half of the cost will not be invoiced.
If the project is not successful and you would like to try again, we are able to make a second attempt for half the cost of the project.
Downstream Application Insurance
As an additional risk mitigation option, users can specify a few downstream applications as the success criteria. Any additional criteria needs to be mentioned at the beginning of the project. Please note that this option would cost extra depending on the downstream applications chosen.
If the antibody does not work for the requested downstream application, then the customer will pay a reduced price for the project.
We respect our customer's confidential information and guarantee that all intellectual property remains with the customer.
| Milestone | Duration |
|---|---|
| Plasmid Synthesis | 14 days |
| Antigen Expression | 10 days |
| Immunization First Shot | 3 weeks + 7 days
3 weeks: The time interval between the first immunization and the second immunization 7 days: Shipping and pre-immunization preparation work |
| Immunization Second Shot | 2 weeks |
| Immunization Third Shot | 2 weeks |
| Immunization Fourth Shot | 2 weeks + 5 days
*The extra 5 days is for bleeding and shipping |
| Serum Purification And Testing | 1 week |
| Deliver Reports | 1 day
Deliverables: WB, IHC test report, 1mg of the purified antibody, and 1mg of purified recombinant protein *Shipping may take 7-10 business days |
Service Details
| Phase | Description/Timeline |
|---|---|
| Phase I Antigen Preparation |
Package 1: Customer Provides Antigen Timeline: 1 week for delivery Requirements: The concentration of the antigen should be at least 0.5 mg/mL and the amount needed is at least 5mg. For the synthetic polypeptide antigen, please provide conjugated peptides (12-22aa) and LC/MS data. The purity should be at least 90%. For the recombinant protein antigen, please provide SDS-PAGE analysis report. The purity should be at least 85%. Also, please tell us your desired end application (optional). Package 2: Boster provides antigen Timeline: 4 weeks Requirements: Immunogen sequence and your desired end application (optional) We can produce synthetic polypeptide antigen and recombinant protein antigen. If you have questions about which one is better for your particular needs or you want to use other types of antigen, please contact us. |
| Phase II Animal Immunization |
Timeline: 10 weeks Conjugated peptides or recombinant proteins are used to immunize 2 rabbits.
|
| Phase III Antigen Affinity Purification and Validation |
Timeline: 1 week Package 1: If more than 1mg antigen is provided by the customer, we can do affinity purification at no additional costs. You have the option of protein A, protein G , or ion-exchange. Package 2: Antigen Affinity Purification: Antibody will be validated on Western Blot and IHC-P (if applicable). Validation: If the antibody reacts to the direct ELISA, then the project will be considered successful. |
| Deliverables | Package 1
Package 2
|
Boster Guarantee
Expertise
Team of experts with decades of combined experience dedicated to producing your polyclonal antibody.
Communication
A dedicated point of contact for you throughout the polyclonal project. Weekly project updates available.
Validation
Basic validation via direct ELISA. Advanced application-specific validation available upon request.
Additional Services
Antigen Synthesis
Contact us for pricing
Instead of providing us the immunogen, we can produce synthetic polypeptide antigen and recombinant protein antigen for your target of interest. This service takes 1 additional month.
Custom Screening Criteria
Contact us for pricing
We can conduct additional screening methods per your requirement, such as testing anti/sera and or culture supernatants in chosen samples on WB, IHC, Flow, etc.
Blocking Peptide
Contact us for pricing
If you require a blocking peptide for your developed antibody, we can help you with that.
Pre-bleed Screening
Contact us for pricing
Get 5ml of pre-bleed for screening purposes prior to commencing with custom-based projects. If you are unfamiliar with this procedure, please inquire.
About Boster Biological Technology
1993
Serving scientists ever since
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Publications citing Boster products
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Average ratings on Biocompare.com