Custom Polyclonal Antibody Production Service

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Service Packages Pricing

Package 1

$800

User Provide Antigen

The most economical solution on the market for custom polyclonal antibody using user provided antigen. Basic screening via direct ELISA.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 2 rabbits
  • antibody purification
  • Shipping and handling
  • Deliverables:
  • Internal test result images will be sent to the customer
  • A small sample will be sent to be validated by customer after we have tested it on WB and IHC internally internal testing.
  • 1mg of the purified antibody
  • 1mg of blocking peptide or 50ng of recombinant protein
  • VIP status at Bosterbio.com

Package 2

$900

Boster Provide Antigen

Do not have an antigen? No worries. We will synthesize an antigen for you for only $100.

  • Dedicated project manager
  • Weekly updates on project status
  • Antigen synthesis
  • Immunizing 2 rabbits
  • Antigen Affinity Purification
  • Shipping and handling
  • Deliverables:
  • Internal test result images will be sent to the customer
  • A small sample will be sent to be validated by customer after we have tested it on WB and IHC internally internal testing.
  • 1mg of the purified antibody
  • 1mg of blocking peptide or 50ng of recombinant protein
  • VIP status at Bosterbio.com


Payment information/options

The way that payment works is that you will be invoiced half the cost when we start the gene syntheses for the immunogen. By default, antibody success is dependent on the reaction of the antigen that the user provides on the direct ELISA assay. The other half will be invoiced upon success. If for any reason you do not think the antibody generated successfully fulfills the success criteria, then please let us know. If we agree that this project is unsuccessful, then other half will not be invoiced.


If the project is not successful, if you wish, then we are able to make a second attempt for half of the cost of the project.

Downstream Application Insurance

As an additional risk mitigation option, users can specify a few downstream applications as the success criteria. Any additional criteria needs to be mentioned in the beginning of the project. Please note that this option would cost extra based on the downstream applications chosen.
If the antibody does not work for the requested downstream appliaction, then the customer will pay a reduce priced for the project.

Service Details

Phase Description/Timeline
Phase I

Antigen Preparation
Package 1: Customer Provide Antigen
Timeline: 1 week for delivery.
Requirements: The concentration of the antigen should be at least 0.5 mg/mL and the amount needed is at least 5mg. For the synthetic polypeptide antigen, please provide conjugated peptides (12-16aa) and LC/MS data. And the purity is at least 90%. For the recombinant protein antigen, please provide SDS-PAGE analysis report. And the purity is at least 85%. Also, tell us your desired end application (Optional).

Package 2: Boster provides antigen
Timeline: 4 weeks
Requirements: Immunogen Sequence and your desired end application (Optional)

We can produce synthetic polypeptide antigen and recombinant protein antigen. If you have questions about which one is better for your particular needs or want to use other types of antigen, please contact us.
Phase II

Animal immunization
Timeline: 10 weeks.
Conjugated peptides or recombinant proteins are used to immunize 2 rabbits.
  • Week 0: Bleed 3-5 ml (yields 1-2 ml pre-immune serum) Immunize with 500 µg/rabbit antigen in CFA.
  • Week 3: Immunize with 250 µg antigen / rabbit in IFA.
  • Week 5: Immunize with 250 µg antigen / rabbit in IFA.
  • Week 6: If the ELISA OD is < 2.0 at a serum dilution of 1:1000, and OD < 1.0 at a serum dilution of 1:3000, the antibody titer is considered low, and we will immunize again with 250 µg antigen/rabbit in IFA.
  • Week 7: 1st Production bleed 10-15 ml if test was positive. ELISA and WB test again if previous tests were negative.
  • Week 8: Test bleed 1-2 ml. ELISA, Western blotting (WB) and Immunohistochemistry (Paraffin-embedded section) (IHC) on appropriate cells / tissue
  • Week 9: If previous tests were negative, immunize with 250 µg antigen/rabbit in IFA
  • Week 10: Terminating bleed 60 -100 ml/rabbit. Test bleed 1-2 ml. ELISA, Western blotting (WB) and Immunohistochemistry (IHC) on appropriate cells / tissue.
Phase III

Antigen Affinity Purification and Validation
Timeline: 1-2 weeks

Package 1: If more than 1mg antigen is provided by the customer, we can do affinity purification at not additional costs. You have the option of protein A, protein G ,or ion-exchange.
Package 2: Antigen Affinity Purification: Antibody will be validated on Western Blot and IHC-P (if applicable)
Validation : If the antibody reacts to the direct ELISA, then the project will be considered successful.
Deliverables

    Package 1

  • Internal test result images will be sent to the customer.
  • A small sample will be sent to the customer to validate on their end after we have tested it on WB and IHC-P internally.
  • 1mg of the purified antibody

  • Package 2

  • Internal test result images will be sent to the customer
  • A small sample will be sent to the customer to validate on their end after we have tested it on WB and IHC internally
  • 1 mg of the purified antibody
  • 1 mg of blocking peptide or 50ng recombinant protein

Boster Guarantee

Expertise

Team of experts with decades of combined experience dedicated to producing your polyclonal antibody.

Communication

A dedicated point of contact for you throughout the polyclonal project. Weekly project updates available.

Validation

Basic validation via direct ELISA. Advanced application-specific validation available upon request.

Additional Services

Antigen Synthesis

$100

Instead of providing us the immunogen, we can produce synthetic polypeptide antigen and recombinant protein antigen for your target of interest.This service takes 1 additional month.
Please note that this pricing is only valid for this custom polyclonal service and is not a stand alone service.

Custom Screening Criteria

Contact us for pricing

We can conduct additional screening methods per your requirement. Such as testing anti/sera and or culture supernatants in chosen samples on WB, IHC, Flow etc.

Blocking Peptide

Contact us for pricing

If you require a blocking peptide for your developed antibody, we can help you with that.

About Boster Biological Technology

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