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Expert Design for High-Afnity Custom Polyclonal Antibodies
Our 25+ years of experience in antibody design and production will ensure you the best chance of getting a high afnity polyclonal antibody that works for your application. Our team of immunology experts will be with you every step of the way.
Antigen design and expression overview
Design
We will design the antigen using our decades of experience in immunogen design, taking consideration of a variety of factors including sequence homology, post-translational modifcation, secondary/tertiary structure and epitope localization.
Expression
We recombinantly express the antigen with our proprietary protein folding technique to ensure best tertiary structure accuracy of the antigen to the target protein’s natural form.
User Provided Antigen
If you decide to provide your own recombinant protein or peptide antigen, we need more than 8 mg of antigen with 80%+ purity, at above 1mg/ml concentration.
Immunization & Purification
4mg of antigen will be used in immunization and 4mg of antigen will be used in a nity puri cation. If you only have 4mg of antigen we can accommodate by using protein A and protein G for puri cation, upon your request.
Immunization schedule overview
Bosterbio’s standard immunization protocol for polyclonal antibody production is optimized to produce high-afnity antibodies. We could adjust the protocol to produce the antibody faster upon your special request, waiving our afnity guarantee.
The protocol uses two New Zealand White rabbits that are Specifc-Pathogen-Free. Immunization injections use Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA). The following table shows the standard protocol and deliverables.
Design
1. Pre-immune serum: if ordered, pre -immune serum will be delivered before the beginning of the project.
2. Test-bleed at 2nd immunization booster (3rd injection): we will afnity purify the bleed and test the antibody for immune reaction for the antigen. The report will be included in the fnal antibody production report. Upon special request we can also provide a vial of purifed sample antibody from this bleed to you to test.
3. Antibody purifcation and validation report: after fnishing the 3rd immunization booster (4th injection), we will collect the bleeds and test the purifed antibody in direct ELISA assay against the immunogen. The results will be written into a report and provided to you.
4. Antibody purifcation and validation report: after fnishing the 3rd immunization booster (4th injection), we will collect the bleeds and test the purifed antibody in direct ELISA assay against the immunogen. The results will be written into a report and provided to you.
Highly Specifcity Antibody
Both the test bleed and the fnal delivery of antibodies are immunoafnity purifed using antigen afnity chromatography. Guaranteed high afnity—we guarantee that at 1:1000 dilution ration the antibody can detect as low as 1ng of the protein antigen, or 100ng of the peptide antigen, in a direct ELISA.
Send us your inquiry to [email protected]
and our team will conduct a feasibility assessment at no charge
Project Overview
Phase 1: Antigen Synthesis
Add Recombinant Antigen
If you need some of the antigen we generate for your project.
Add Blocking Peptide
If you need some of the antigen we can provide it.
Antigen Design & Development
Included
Our team will help you design the best antigen for your intended application. We consider multiple factors uniqueness of the antigen hydrophobic/hydrophilic composition secondary structure (alpha-helical tendencies, beta-turn tendencies and all) and a variety of other factors. Based on the nature of your target application, and the chemical nature of the antigen, we will design an immunogen unique to your purpose.
Phase 1 duration: Approximately 4 weeks/28 days
Phase 2: Immunization
Add Pre-immune serum screening
Custom antibody projects can fail due to the animal having existing immunity or immune-tolerance towards your antigen of interest. As an optional service, we can send you pre-immune sera to screen for this issue before your project starts. We can also perform the screening for you.
Pre-immune serum screening
Add Rabbits
Adding more rabbits will increase your success rate
and increase the yield.
Immunization
2 Rabbits Included
Immunization preparation Day 1
Preparing antigen with CFA, preparing rabbits.
Immunization primary injection Day 8
10 subcutaneous immune boost injections, 0.5mg of antigen in CFA each
Immunization 2nd shot Day 22
4 subcutaneous immune boost injections, 0.25mg of antigen in IFA each
Immunization 3rd shot Day 36
4 subcutaneous immune boost injections, 0.25mg of antigen in IFA each
Immunization 4th shot Day 51
4 subcutaneous immune boost injections, 0.25mg of antigen in IFA each
Add Validation
Boster Bio ofers a full service antibody validation CRO.
We can perform IHC, WB, IF, ICC and FLOW.
Sera collection and ELISA test Day 65
Direct ELISA Included
Testing the anti-sera via ELISA,
decide to continue or terminate project
Add Application Insurance
Additional insurance you can purchase: 100% guarantee that your
antibody will work in your application or unique assay.
Serum purifcation & fnal deliveryDay 72
Afnity Purifcation Included
Deliverables: ELISA test report, 6mg of the purifed antibody
*Shipping may take 7-10 business days.
Add Conjugation
Additional service: conjugate your antibody
to a conjugate of your choice
Final DeliveryDay 80
Phase 2 duration: Approximately 12 weeks/80 days
Summary
Timeline
User provided antigen: 11 weeks plus shipping Boster Bio produces antigen: 15 weeks plus shipping *times are approximations, actual timeline may vary.
Included Items Standard Package $1300
- Antigen Design
- Antigen Production
- Immunization of 2 Rabbits
- Direct ELISA validation
- Afnity Purifcation
- Deliverables: 3-5mg antibody
Additional Items
| Recombinant Antigen | 1mg/$200 |
| Blocking Peptide | 100ug/$150 |
| Pre-Immune serum bleed | $100 / 1ml per rabbit |
| Additonal Rabbits | $200 each |
| Additional Validation | See Antibody Validation Page |
| Conjugation | 100ul/$50 inquire bulk |
| Application Insurance | $1,300 |
| Custom Bufer | Inquire |
Guarantee
We guarantee that at 1:1000 dilution ratio on the antibody can detect
as low as 1ng of the protein antigen, or 100ng of the
peptide antigen, in a direct ELISA.
Minimum yield of 3mg
If failure at antigen expression you only pay $250
If failure at direct ELISA test you only pay $500
Send us your inquiry to [email protected]
and our team will conduct a feasibility assessment at no charge