ELISA KITS IMMUNOASSAYS

Enzyme-linked Immunosorbent Assay Kits

Boster Provides High Quality ELISA Kits That Are Cited In 6000+ Publications

Boster Biological Technology has 25+ years of experience in manufacturing ELISA kits and antibodies. As a trusted ELISA kit company, we proudly offer 1000+ ELISA test kits in both sandwich ELISA format and competitive ELISA format, covering human, mouse, rat, and various other mammalian species. As life scientists, we understand the competitive and challenging nature of your work, and we do our best to support your experiments, from design to data analysis. Boster's enzyme-linked immunosorbent assays go through painstaking robust validation to ensure the highest specificity, sensitivity, and stability. Use Boster’s ELISA assay kits and you will have reproducible, publishable data lot after lot. CiteAb.com, a big data research company, rated us as the fastest growing antibody company in the world in terms of publication citations. As of 2019, our ELISA kits alone have been cited in 6,000+ publications globally.

"Great TNF-Alpha Kit from Boster! Very easy to use."

--Lab Technician from Appalachian State University

"No complaints. A simple, effective ELISA from Boster Bio. I'd recommend the kit."

--Research Scientist from University of Wisconsin

Browse all Picokine™ ELISA Kits

What Is An ELISA Kit?

ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the ELISA (Enzyme-Linked Immunosorbent Assay). ELISA is a plate-based experiment designed to detect and quantify molecules, such as proteins, antibodies, hormones, peptides and sometimes small molecules like vitamins and coenzymes. Antibodies linked with enzymes are used to quantify the results. Various formats (see below) exist and the antibody antigen interaction principle is different for each format.

Boster ELISA Kit Features

Here are some quick facts about our ELISA kits

Sensitivity

Sensitivity

Boster's Picokine™ ELISA kits are made with high affinity antibodies that can detect native form proteins with picogram and subpicogram level sensitivity.

Specificity

Specificity

Boster's QC department validates our ELISA kits against proteins in relevant superfamilies and proteins with similar immunogenicities to ensure specificity to the analytes of interests.

Selection

Selection

Our 2300+ ELISA kits are validated in multiple sample matrices from serum and saliva to urine and feces, ensuring wide application ranges for you to select from.

Service

Service

Boster has been serving the research community since 1993 and cited by 23,000+ publications. Our team of experts are dedicated to provide you the best customer service.

How Does The ELISA Kit Work?

The key step, immobilization of the antigen of interest, can be accomplished by direct adsorption to the assay plate or indirectly via a capture antibody that has been attached to the plate. The antigen is then detected either directly (enzyme-labeled primary antibody) or indirectly (enzyme-labeled secondary antibody). The detection antibodies are usually labeled with alkaline phosphatase (AP) or horseradish peroxidase (HRP). A large selection of substrates is available for performing the ELISA with an HRP or AP conjugate. The choice of substrate depends upon the required assay sensitivity and the instrumentation available for signal-detection (spectrophotometer, fluorometer, or luminometer). Detection is achieved by measuring the enzyme activity. One method is through incubation with TMB substrate which can be detected via the converted substrate’s light absorbance at 450nm. Alternatively, the enzyme can catalyze chemiluminescent substrate, triggering a strong luminescent reaction which can be quantified.

Typical ELISA Protocol Workflow

Boster's ELISA kits contain all the reagents one needs to conduct a sandwich ELISA, with pre-coated ELISA plates. Case management shouldn’t take hours, so we designed it to be easy. With the following simple steps, all your documents will fall in the right place, so your team can focus on the things that matter:

Coating
Polystyrene plate is treated with a solution of either antigen or antibody

Blocking
An unrelated protein-based solution is used to cover all unbound sites on the plates.

Detection
Enzyme-conjugated antibody or antigen binds specifically to the target antigen or antibody.

Read Results
Substrate is added and the signal produced by the enzyme-substrate reaction is measured.

Featured Products

Check out Boster's popular ELISA kits

About Boster Biological Technology

Here are some quick facts about bosterbio.com

26
Years

2300+
Publications

4.8 / 5
Rating on biocompare.com

16000+
Antibodies

900+
ELISA Kits

“Fastest Growing Antibody Company” – CiteAb.Com

Boster ELISA kits are well-cited

Boster antibodies and ELISA kits are cited over 23,000+ times in publications around the world. According to the big data research company CiteAb.com, Bosterbio is globally the fastest growing antibody company in terms of the number of academic publication citations. For Picokine® ELISA kits specifically, an estimate of 6000+ publications are available. You can find specific citations for ELISA kits and antibodies on each product page.

Testimonials

What life scientists are saying about Boster ELISA kits

Partners & Customers

human fibronectin elisa kit

"How To Become An Expert" Experiment EBook Series

Do you want to know more about ELISA?

Download our popular eBook for ELISA: How to Become an ELISA Expert in 4 days. You can find it along with our other experiment guides in the link below.
Boster offers research reagents, antibodies, and ELISA kits based on 26+ years of experience manufacturing these specific products. We are application experts that know everything about Western blotting, Immunohistochemistry, and more importantly, ELISA, and we are here to help you with everything we've got. One of our most popular educational material is our How to Become an Expert eBook series.

In the How to Become an ELISA Expert in 4 days eBook, you can expect the following:

  • ELISA Principle: Having a solid understanding of how ELISA works significantly puts you ahead of the learning curve. We have everything you need to know from sample preparation to data analysis.
  • Optimized Protocols: ELISA is complex and prone to errors in many ways. Our step-by-step protocols will guide you to generate reproducible, high quality data.
  • 150+ Troubleshooting Advice: Having our troubleshooting guide that addresses all kinds of problems can save you a lot of precious time and headache.
  • Frequently Asked Questions: Learning from your peers’ experiences can bring you novel perspectives and help you avoid common mistakes.
Download

ELISA Kits FAQs

Don't see what you want? Ask us at [email protected]

Q.1 How do I troubleshoot low signal in ELISA results?

A number of problems commonly arise during ELISA that can result in low signal and poor color development. Use our troubleshooting tips to help you improve your experiment and get better data. For more information, visit ELISA Troubleshooting Low Signal.

Q.2 What is matrix effect and how does it affect my results?

Matrix effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results. Read some tips to reduce matrix effect.

Q.3 How many samples can I run on a 96-well ELISA plate?

On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate. Learn more about the number of samples tested per micotiter plate.

Q.4 Is the plate separable? Can I use only part of the kit and save the rest for later?

Yes, the ELISA kit plate is separable if the kit description says that there are removable strips. There are 12 strips of 8 wells each, all removable from the plate.

Q.5 What is the concentration of sodium azide in your ELISA kits?

Our ELISA kits contain 0.02% sodium azide. All of the components, except TMB color developing reagent and stop solution, contain 0.02% sodium azide as the preservative.

Q.6 Do your ELISA kits come with sealants or plate covers?

The kit can be used within a month sequentially if it is opened and stored at 4 degrees. There is no need to use sealants. The plate can be packaged with an aluminum foil bag. For other reagents, keep the bottles tightly closed.

Q.7 For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals?

This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.

Q.8 The kit does not include wash solution. What should I do?

Our ELISA kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder, SKU: AR0030).

Q.9 What is the well depth of the 96 well plates for the ELISA kits?

The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.

Q.10 Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine?

We test serum and plasma routinely, and there is very little difference between serum, plasma, and whole blood. The whole blood also contains proteins we need to test. In theory, the kit can be used to test whole blood.

Q.12 Does this ELISA kit contain any product produced in humans or primates?

None of the components in our ELISA kits are produced in humans or primates.

Q.13 Would your kits work normally with samples from Wistar Rat?

Our kits would work normally with samples from Wistar Rat.

Q.14 Will this kit work on tissue samples?

For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is endogenous biotin in the tissue lysate samples using the ELISA kit. • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, then you can work on the tissue sample by using the kit.

Q.15 I left this product out in ambient temperatures for around 5 days. Will the kit be stable?

Our kits should still be stable after being in ambient temperatures for around 5 days. It would not affect the performance of the kit. We suggest you to run the test and contact us if it does not work as expected.

The bubbles in the wells is an uncommon issue, but it would not affect the results of your experiment. Prior to the assay, we suggest washing the plate to remove the bubbles as suggested in the following protocol: Wash the plate 3 times with the 1X wash buffer. Discard the liquid in the wells into an appropriate waste receptacle. Then, invert the plate on the benchtop onto a paper towel and tap the plate to gently blot any remaining liquid. It is recommended that the wells should not be completely dry at any time. Add 300 µl of the 1x wash buffer to each assay well. (For cleaner background, incubate for 60 seconds between each wash.) Repeat steps a-b 2 additional times.

ELISA Kit Related Blogs

Check out Boster's rich collection of experiment tips and technical advice

ELISA Fundamental Principle, How ELISA Works

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface...

Continue Reading

ELISA Sample Preparation Guide

The procedure below provides a general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article...

Continue Reading

An ELISA Guide That You Cannot Miss

This guide will teach you everything you need to become an ELISA expert, including critical review of principle, all-in-one FAQs, and more...

Continue Reading

Boster's Free Troubleshooting Guides

You are at the right place and at the right time for our latest series of troubleshooting handbooks. They are available at your finger tips with just a few clicks...

Continue Reading

Guidelines for Preparing ELISA Standards

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay used to detect the concentration of a specific protein in a liquid sample. Three different types of data output can be obtained...

Continue Reading

Four Types of ELISA Assay

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in...

Continue Reading

ELISA Troubleshooting: Weak Signal

Weak or no color development in an ELISA assay can indicate that the target protein is present in minute quantities in the sample, if at all. It can also mean that there is something wrong with the assay or the reagents that prevents efficient detection....

Continue Reading

ELISA Blocking Optimization

The process of coating an ELISA plate relies on the passive binding activity of the solid phase, which immobilizes biomolecules on the well surface. Without appropriate blocking, the plate would bind the detection antibody alongside the antigen or detection...

Continue Reading

ELISA Data Analysis

After performing an ELISA with a ready-to-use ELISA kit or an antibody pair kit, the data generated must be analyzed to quantitate the target protein concentrations. We discuss the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create the standard curve for analysis.....

Continue Reading

Pitfalls to Avoid for ELISA!

ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages, there are some mistakes that could turn your ELISA experiment sour...

Continue Reading

ELISA Blocking Buffer Optimization

There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between....

Continue Reading

How to Perfect Your ELISA Standards: Protocol and Troubleshooting

At Boster, one common question we get from researchers is, “How do I prepare the ELISA standard?” We’re glad you asked because proper construction of the standard curve is the very first step....

Continue Reading