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Boster Biological Technology has 25+ years of experience in manufacturing ELISA kits and antibodies. As a trusted ELISA kit company, we proudly offer 1000+ ELISA test kits in both sandwich ELISA format and competitive ELISA format, covering human, mouse, rat, and various other mammalian species. As life scientists, we understand the competitive and challenging nature of your work, and we do our best to support your experiments, from design to data analysis. Boster's enzyme-linked immunosorbent assays go through painstaking robust validation to ensure the highest specificity, sensitivity, and stability. Use Boster’s ELISA assay kits and you will have reproducible, publishable data lot after lot. CiteAb.com, a big data research company, rated us as the fastest growing antibody company in the world in terms of publication citations. As of 2019, our ELISA kits alone have been cited in 6,000+ publications globally.
"Great TNF-Alpha Kit from Boster! Very easy to use."
--Lab Technician from Appalachian State University
"No complaints. A simple, effective ELISA from Boster Bio. I'd recommend the kit."
--Research Scientist from University of Wisconsin
ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the ELISA (Enzyme-Linked Immunosorbent Assay). ELISA is a plate-based experiment designed to detect and quantify molecules, such as proteins, antibodies, hormones, peptides and sometimes small molecules like vitamins and coenzymes. Antibodies linked with enzymes are used to quantify the results. Various formats (see below) exist and the antibody antigen interaction principle is different for each format.
Here are some quick facts about our ELISA kits
Boster's Picokine™ ELISA kits are made with high affinity antibodies that can detect native form proteins with picogram and subpicogram level sensitivity.
Boster's QC department validates our ELISA kits against proteins in relevant superfamilies and proteins with similar immunogenicities to ensure specificity to the analytes of interests.
Our 2300+ ELISA kits are validated in multiple sample matrices from serum and saliva to urine and feces, ensuring wide application ranges for you to select from.
Boster has been serving the research community since 1993 and cited by 23,000+ publications. Our team of experts are dedicated to provide you the best customer service.
The key step, immobilization of the antigen of interest, can be accomplished by direct adsorption to the assay plate or indirectly via a capture antibody that has been attached to the plate. The antigen is then detected either directly (enzyme-labeled primary antibody) or indirectly (enzyme-labeled secondary antibody). The detection antibodies are usually labeled with alkaline phosphatase (AP) or horseradish peroxidase (HRP). A large selection of substrates is available for performing the ELISA with an HRP or AP conjugate. The choice of substrate depends upon the required assay sensitivity and the instrumentation available for signal-detection (spectrophotometer, fluorometer, or luminometer). Detection is achieved by measuring the enzyme activity. One method is through incubation with TMB substrate which can be detected via the converted substrate’s light absorbance at 450nm. Alternatively, the enzyme can catalyze chemiluminescent substrate, triggering a strong luminescent reaction which can be quantified.
Boster's ELISA kits contain all the reagents one needs to conduct a sandwich ELISA, with pre-coated ELISA plates. Case management shouldn’t take hours, so we designed it to be easy. With the following simple steps, all your documents will fall in the right place, so your team can focus on the things that matter:
Polystyrene plate is treated with a solution of either antigen or antibody
An unrelated protein-based solution is used to cover all unbound sites on the plates.
Enzyme-conjugated antibody or antigen binds specifically to the target antigen or antibody.
Substrate is added and the signal produced by the enzyme-substrate reaction is measured.
Check out Boster's popular ELISA kits
Here are some quick facts about bosterbio.com
Boster antibodies and ELISA kits are cited over 23,000+ times in publications around the world. According to the big data research company CiteAb.com, Bosterbio is globally the fastest growing antibody company in terms of the number of academic publication citations. For Picokine® ELISA kits specifically, an estimate of 6000+ publications are available. You can find specific citations for ELISA kits and antibodies on each product page.
What life scientists are saying about Boster ELISA kits
Maria Teresa Dell'Anno
This Antibody Works Perfectly!
I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again.
Immunohistochemistry , Spinal cord , Confocal microscope
Verification Of Antibody On Recombinant Protein
We tested the antibody against recombinant GST-tagged BnaA07g20720D protein. As controls, we used another GST-tagged protein GST-MYB25, and GST alone. The antibody was well capable of detecting specifically BnaA07g20720D. We loaded ~100 ng of either GST:BnaA07g20720D, GST:MYB25, or GST alone, and the antibody was very specifically detecting BnaA07g20720D. It did not cross-react at all with the other two proteins. Highly specific. I would highly recommend the antibody for basic western-blot analysis of purified proteins. Probably one can significantly reduce the dilution to 1:10000 to still get a good result. Unfortunately, we did not have the opportunity with good controls to verify how the antibody works on whole plant extracts or for IP experiments.
Western Blot , Recombinant protein expressed in and purified from E. coli , Chemiluminescence
A Good PKC Alpha Antibody For IP And WB
We used Anti-PKC Alpha Picoband™ Antibody for immunoprecipitation experiment from neuronal cells in order to characterize PKC Alpha expression in different conditions. Strong positive band with right mass. Works great without optimization! Good quality and ready for publish Ab
Western Blot , Dorsal root ganglia (neuronal cells), CyDye
Best Calbindin Antibody For IHC In Mouse Cerebellum
I tried this antibody for Purkinje cell labeling in mice cerebellum using IHC-P. Antigen retrieval - 20min boiling in EDTA based buffer. Permeabilization - 0.3% triton for 15min. Blocking - 10% goat serum for 1hr. Primary antibody - 1:250 overnight at 4 Degree C. Secondary antibody - Fluorescence conjugated secondary antibody at 1:250 for 2hr at RT. I was amazed to see the beautiful dendrites, soma and axons. In my hand, this is the best labeling of Purkinje cells I have ever seen using IHC-P. Beautiful labeling of Purkinje cell soma, dendrites and axons. Best antibody I have used till now. Highly recommended for IHC-P.
Immunohistochemistry , Mice Cerebellum , Fluorescence
Good Zebrafish Antibody For Nlz2
Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise.
Immunohistochemistry , Zebrafish embryos 24hpf , Confocal Microscopy
Awesome Kip2 Antibody From Boster Bio
We've been using this Ab for several months, and it's been working great. We have already ordered several vials of this Ab for detecting podocytes in mice kidney. We used an autostainer machine for the process. With this Ab dilution, and using EDTA for Ag retrieval, we had the least background. Easy to use, works every time. Worth it.
Immunohistochemistry , Mice kidney , DAB chromogen
Quantification Of BDNF Post-Transfection In Primary Neuronal Cells
The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes. Provided good reliable quantification of BDNF produced. No interference. Good reproducibility. Reliable estimation, good sensitivity. Reasonable price as well. Would purchase again." (Customer’s Tip: "Don't dilute sample, assay directly.
ELISA , Cell lysate and culture medium
Adiponectin Analysis in Mouse Serum and Culture Media
I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.
ELISA , Serum, tissue homogenate
Good TIMP-1 Antibody for Western Blotting
We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it.
Western Blot , Human NSC cell lysates , ECL chemiluminescence
Peter J. Thompson
This Antibody Gives Strong Membrane Staining
CD47 is broadly expressed in many cell types. This antibody gives strong membrane staining throughout many of the beta cells (Insulin-positive) in the immune cell infiltrated islets of NOD mice at age 12 weeks.
IHC-Paraffin, IHC-cryo , NOD/ShiLtJ mouse pancreas sections, formalin-fixed paraffin embedded , Zeiss Apotome widefield fluorescence microscope
The Signal Was Very Strong And Clean!
Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.
Western Blot , Whole cell extract of pancreatic tumor cell MIA PaACa2 , Enhanced chemiluminescence
Download our popular eBook for ELISA: How to Become an ELISA Expert in 4 days. You can find it along with our other experiment guides in the link below.
Boster offers research reagents, antibodies, and ELISA kits based on 26+ years of experience manufacturing these specific products. We are application experts that know everything about Western blotting, Immunohistochemistry, and more importantly, ELISA, and we are here to help you with everything we've got. One of our most popular educational material is our How to Become an Expert eBook series.
In the How to Become an ELISA Expert in 4 days eBook, you can expect the following:
Check out Boster's rich collection of experiment tips and technical advice
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface...Continue Reading
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ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages, there are some mistakes that could turn your ELISA experiment sour...Continue Reading
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