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Boster Provides High Quality ELISA Kits that are Cited in 6000+ Publications
Boster Biological Technology has 25+ years of experience in manufacturing ELISA kits and antibodies. As a trusted ELISA kit company, we proudly offer 1000+ ELISA test kits in both sandwich ELISA format and competitive ELISA format, covering human, mouse, rat, and various other mammalian species. As life scientists, we understand the competitive and challenging nature of your work, and we do our best to support your experiments, from design to data analysis. Boster's enzyme-linked immunosorbent assays go through painstaking robust validation to ensure the highest specificity, sensitivity, and stability. Use Boster’s ELISA assay kits and you will have reproducible, publishable data lot after lot. CiteAb.com, a big data research company, rated us as the fastest growing antibody company in the world in terms of publication citations. As of 2019, our ELISA kits alone have been cited in 6,000+ publications globally.
Browse all Picokine™ ELISA KitsWhat is an ELISA Kit?
ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the ELISA (Enzyme-Linked Immunosorbent Assay). ELISA is a plate-based experiment designed to detect and quantify molecules, such as proteins, antibodies, hormones, peptides and sometimes small molecules like vitamins and coenzymes. Antibodies linked with enzymes are used to quantify the results. Various formats (see below) exist and the antibody antigen interaction principle is different for each format.
Boster ELISA Kit Features
Here are some quick facts about our ELISA kits
Sensivity
Boster's Picokine™ ELISA kits are made with high affinity antibodies that can detect native form proteins with picogram and subpicogram level sensitivity.
Specificity
Boster's QC department validates our ELISA kits against proteins in relevant superfamilies and proteins with similar immunogenicities to ensure specificity to the analytes of interests.
Selection
Our 2300+ ELISA kits are validated in multiple sample matrices from serum and saliva to urine and feces, ensuring wide application ranges for you to select from.
Service
Boster has been serving the research community since 1993 and cited by 23,000+ publications. Our team of experts are dedicated to provide you the best customer service.
How does the ELISA kit work?
The key step, immobilization of the antigen of interest, can be accomplished by direct adsorption to the assay plate or indirectly via a capture antibody that has been attached to the plate. The antigen is then detected either directly (enzyme-labeled primary antibody) or indirectly (enzyme-labeled secondary antibody). The detection antibodies are usually labeled with alkaline phosphatase (AP) or horseradish peroxidase (HRP). A large selection of substrates is available for performing the ELISA with an HRP or AP conjugate. The choice of substrate depends upon the required assay sensitivity and the instrumentation available for signal-detection (spectrophotometer, fluorometer, or luminometer). Detection is achieved by measuring the enzyme activity. One method is through incubation with TMB substrate which can be detected via the converted substrate’s light absorbance at 450nm. Alternatively, the enzyme can catalyze chemiluminescent substrate, triggering a strong luminescent reaction which can be quantified.
Typical ELISA Protocol Workflow
Boster's ELISA kits contain all the reagents one needs to conduct a sandwich ELISA, with pre-coated ELISA plates. Case management shouldn’t take hours, so we designed it to be easy. With the following simple steps, all your documents will fall in the right place, so your team can focus on the things that matter:
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Coating
Polystyrene plate is treated with a solution of either antigen or antibody.
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Blocking
An unrelated protein-based solution is used to cover all unbound sites on the plates.
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Detection
Enzyme-conjugated antibody or antigen binds specifically to the target antigen or antibody.
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Read Results
Substrate is added and the signal produced by the enzyme-substrate reaction is measured.
Featured Products
Check out Boster's popular ELISA kits
About Boster Biological Technology
Here are some quick facts about bosterbio.com
26
Years
23,000+
Publications
4.8/5
Rating
16,000+
Antibodies
2300+
ELISA Kits
“Fastest Growing Antibody Company” – CiteAb.com
Boster ELISA kits are well-cited
Boster antibodies and ELISA kits are cited over 23,000+ times in publications around the world. According to the big data research company CiteAb.com, Bosterbio is globally the fastest growing antibody company in terms of the number of academic publication citations. For Picokine® ELISA kits specifically, an estimate of 6000+ publications are available. You can find specific citations for ELISA kits and antibodies on each product page.
Testimonials
What life scientists are saying about Boster ELISA kits
"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide..
Milos Lazic,
JECURE THERAPEUTICS, JECURE THERAPEUTICS, SCIENTIST II
I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks...
Jason Somarelli
DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE
This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon.
Tho Pham
UCONN, NUTRITIONAL SCIENCES, GA
I used this kit to detect a recombinant IL1RA in rat serum and it worked very well. The procedure was easy to follow and my standard curve was right on target. If needed we will order this kit and use it again. The kit was able to detect our rIL1RA but was less sensitive in detecting...
Unattributed Review
Director
We used this kit and the BDNF kit, because we were looking at neurogenesis within the hippocampus. We used the ELISA to complement our immunohistochemistry. It was easy to optimize the kits and the instructions were extremely easy to follow. Additionally, the instructions do not include using samples...
Hannah Oakes
EAST TENNESSEE STATE UNIVERSITY, BIOMEDICAL SCIENCES, GRADUATE ASSISTANT
This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit...
Charles Stopford
UNIVERSITY OF NORTH CAROLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST
This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased...
J L
UIC, DENTISTRY, SCIENTIST
The TFF1 ELISA kit from Boster was competitively priced and worked with cell culture media taken from MCF7 cells...The kit is very sensitive, detecting TFF1 protein at levels of 10pg/mL. Good ELISA kit for human TFF1 protein
Lu Yang
UCSD, CMMW, GRADUATE STUDENT
The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor...
Unattributed Review
ELISA Kits FAQs
Don't see what you want? Ask us at [email protected]
A number of problems commonly arise during ELISA that can result in low signal and poor color development. Use our troubleshooting tips to help you improve your experiment and get better data. For more information, visit ELISA Troubleshooting Low Signal.
Matrix effect occurs when the target antigen interacts with matrix components in plasma or serum samples. These matrix components can be endogenous biological components such as phospholipids, carbohydrates, and metabolites. Matrix components can reduce the binding of the antibody to the target protein, or non-specifically bind the antibody, generating weak or noisy results. Read some tips to reduce matrix effect.
On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate. Learn more about the number of samples tested per micotiter plate.
Yes, the ELISA kit plate is separable if the kit description says that there are removable strips. There are 12 strips of 8 wells each, all removable from the plate.
Our ELISA kits contain 0.02% sodium azide. All of the components, except TMB color developing reagent and stop solution, contain 0.02% sodium azide as the preservative.
The kit can be used within a month sequentially if it is opened and stored at 4 degrees. There is no need to use sealants. The plate can be packaged with an aluminum foil bag. For other reagents, keep the bottles tightly closed.
This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Our ELISA kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder, SKU: AR0030).
The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
We test serum and plasma routinely, and there is very little difference between serum, plasma, and whole blood. The whole blood also contains proteins we need to test. In theory, the kit can be used to test whole blood.
It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
None of the components in our ELISA kits are produced in humans or primates.
Our kits would work normally with samples from Wistar Rat.
For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is endogenous biotin in the tissue lysate samples using the ELISA kit.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
If no signal is produced, then you can work on the tissue sample by using the kit.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
If no signal is produced, then you can work on the tissue sample by using the kit.
Our kits should still be stable after being in ambient temperatures for around 5 days. It would not affect the performance of the kit. We suggest you to run the test and contact us if it does not work as expected.
The bubbles in the wells is an uncommon issue, but it would not affect the results of your experiment. Prior to the assay, we suggest washing the plate to remove the bubbles as suggested in the following protocol: Wash the plate 3 times with the 1X wash buffer. Discard the liquid in the wells into an appropriate waste receptacle. Then, invert the plate on the benchtop onto a paper towel and tap the plate to gently blot any remaining liquid. It is recommended that the wells should not be completely dry at any time. Add 300 µl of the 1x wash buffer to each assay well. (For cleaner background, incubate for 60 seconds between each wash.) Repeat steps a-b 2 additional times.
ELISA Kit Related Blogs
Check out Boster's rich collection of experiment tips and technical advice
ELISA Fundamental Principle, How ELISA Works
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface...
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ELISA Sample Preparation Guide
The procedure below provides a general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article...
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An ELISA Guide That You Cannot Miss
This guide will teach you everything you need to become an ELISA expert, including critical review of principle, all-in-one FAQs, and more...
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Boster's Free Troubleshooting Guides
You are at the right place and at the right time for our latest series of troubleshooting handbooks. They are available at your finger tips with just a few clicks...
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Guidelines for Preparing ELISA Standards
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay used to detect the concentration of a specific protein in a liquid sample. Three different types of data output can be obtained...
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Four Types of ELISA Assay
Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in...
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ELISA Troubleshooting: Weak Signal
Weak or no color development in an ELISA assay can indicate that the target protein is present in minute quantities in the sample, if at all. It can also mean that there is something wrong with the assay or the reagents that prevents efficient detection....
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ELISA Blocking Optimization
The process of coating an ELISA plate relies on the passive binding activity of the solid phase, which immobilizes biomolecules on the well surface. Without appropriate blocking, the plate would bind the detection antibody alongside the antigen or detection...
Continue Reading ELISA Data Analysis
After performing an ELISA with a ready-to-use ELISA kit or an antibody pair kit, the data generated must be analyzed to quantitate the target protein concentrations. We discuss the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create the standard curve for analysis.....
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Pitfalls to Avoid for ELISA!
ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages, there are some mistakes that could turn your ELISA experiment sour...
Continue Reading ELISA Blocking Buffer Optimization
There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between....
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How to Perfect Your ELISA Standards: Protocol and Troubleshooting
At Boster, one common question we get from researchers is, “How do I prepare the ELISA standard?” We’re glad you asked because proper construction of the standard curve is the very first step....
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