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Paraformaldehyde Cooling and Dehydration
Liquid Paraffin Section
Section Slicing and Incubation
Snap Freezing and OCT Embedding
Note: This fixation procedure using paraformaldehyde and formalin fixatives may cause autofluorescence in the green spectrum. In this case, you may try fluorophores in the (i) red range or (ii) infrared range if you have an infrared detection system.