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Product Info Summary
| SKU: | A07839 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Ribosomal Protein S8 RPS8 Antibody
SKU/Catalog Number
A07839
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Ribosomal Protein S8 RPS8 Antibody catalog # A07839. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ribosomal Protein S8 RPS8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07839)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPS8. AA range:111-160
Reactive Species
A07839 is reactive to RPS8 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
24.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07839 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Ribosomal Protein S8 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from A549, 293, and COLO cells, using RPS8 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from Jurkat cells using RPS8 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Ribosomal Protein S8 RPS8 Antibody (A07839)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Ribosomal Protein S8 RPS8 Antibody
Question
Is there a BSA free version of anti-Ribosomal Protein S8 antibody A07839 available?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ribosomal Protein S8 antibody A07839 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is this A07839 anti-Ribosomal Protein S8 antibody reactive to the isotypes of RPS8?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The immunogen of A07839 anti-Ribosomal Protein S8 antibody is Synthesized peptide derived from the Internal region of human Ribosomal Protein S8. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-Ribosomal Protein S8 antibody A07839 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
The anti-Ribosomal Protein S8 antibody (A07839) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon adenocarcinoma using anti-Ribosomal Protein S8 antibody A07839. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-21
Question
Does anti-Ribosomal Protein S8 antibody A07839 work on horse WB with colon adenocarcinoma?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
Our lab technicians have not validated anti-Ribosomal Protein S8 antibody A07839 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Ribosomal Protein S8 antibody A07839 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse colon adenocarcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-16
Question
I have a question about product A07839, anti-Ribosomal Protein S8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Anderson
Verified customer
Asked: 2018-06-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07839 anti-Ribosomal Protein S8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-04
