CIP2A · Western blot design guide

CIP2A Western Blot Planning Guide

Plan a CIP2A Western blot around the catalog-observed 102.2 kDa band, image-backed PB9714 evidence, HPA controls, and verified protocol records.

Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use

Western blot protocol sheet for CIP2A (CIP2A): expected band 102.2 kDa, antibody PB9714, and PMC-cited SDS-PAGE protocol steps

Band Protocols Controls Antibodies

CIP2A Western Blot Experimental Design Guide

Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.

Must know before running

	Expected band	102.2 kDa
	Observed band	Not reported — verify product WB image
	Gel	8-10%
	Positive control ⓘ	Adipose tissue
	Negative control ⓘ	Spleen

Important caveats

Reasons your observed band may differ from the expected size.

ⓘ	Calculated mass	102.2 kDa
ⓘ	Localization	Cytoplasm / Chromosome
ⓘ	Processing / PTM	Record-dependent
ⓘ	Reactivity	Human / Mouse

Sample controls for CIP2A Western blot

🧪Use Adipose tissue as the first positive-control candidate and Spleen as the HPA Not detected negative candidate.

Positive control: Adipose tissue (High)

Negative control: Spleen (Not detected)

HPA protein score determines control status; other expression data is supporting context only.

HPA tissue expression evidence for CIP2A

Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.

Positive expression · recommended positive controls

Tissue	Cell type	Level	Evidence	Source
Adipose tissue	Reported tissue cells	High	Protein (HPA)	HPA →
Breast	Reported tissue cells	High	Protein (HPA)	HPA →
Bronchus	Reported tissue cells	High	Protein (HPA)	HPA →
Cerebellum	Reported tissue cells	High	Protein (HPA)	HPA →

Undetected expression · recommended negative controls

Tissue	Cell type	Level	Evidence	Source
Spleen	Reported tissue cells	Not detected	Protein (HPA)	HPA →

Section 1

What Is the Expected CIP2A Western Blot Band Size?

Use the product-observed 102.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.

What am I looking at on my blot?
102.2 kDa	Matches the authoritative product WB observation.
102.2 kDa calculated	Use as UniProt context, not as a replacement observed band.
Unexpected additional signal	Do not assign identity without orthogonal positive/negative controls.

💡Expected CIP2A appearancePlan around 102.2 kDa and keep the calculated mass as supporting context.

How each factor affects band size
Catalog-observed band	102.2 kDa; use this as the primary experimental expectation.
Calculated mass	102.2 kDa from UniProt Q8TCG1; retain as context.
Gel selection	8-10%; shared with the recommended protocol and poster.
Specificity check	Compare the lead HPA positive and negative controls with PB9714.

Why is my band missing or off?
Situation	Likely cause	Next action
102.2 kDa	Matches the authoritative product WB observation.	Confirm with orthogonal controls and the linked product record.
Additional band	May reflect processing, modification, or non-specific signal.	Run a dilution series and compare positive/negative controls.
Weak signal	Target abundance or transfer may be limiting.	Verify transfer, increase positive-control abundance, and bracket exposure.

Sources: UniProt Q8TCG1 Human Protein Atlas product datasheet (PB9714)

Experiment evidence

Western blot images showing CIP2A band

PB9714 · CIP2A — observed 102.2 kDa WB validation

PB9714 · CIP2AObserved 102.2 kDa · product-specific WB image

← swipe / scroll for more images →

Real WB data behind the band-size expectations in this section.

Section 2

Real Curated CIP2A Western Blot Protocols

Start with the molecular-weight rule, then compare verified publication-derived conditions.

Recommended Western blot protocol parameters
Sample / lysate	Adipose tissue
Gel %	8-10%
Load	20-30 µg total protein per lane
Transfer	Wet/tank, extended transfer
Membrane	0.45 µm PVDF
Blocking	5% non-fat milk or 5% BSA in TBST
Primary	PB9714 at datasheet starting dilution
Primary incubation	Overnight at 4 °C with gentle agitation
Secondary	Species-matched HRP conjugate at validated dilution
Wash	3 × 5 min in TBST
Detection	Chemiluminescent substrate
Exposure	Bracket exposures to avoid saturation

Section 4

Advanced CIP2A Western Blot Tips

Deeper troubleshooting and optimisation questions for CIP2A, answered from its protein features.

Which band should guide the blot?

Use 102.2 kDa, the observation attached to the authoritative PB9714 WB record.

How should calculated mass be interpreted?

Treat the UniProt calculated mass as context; it does not replace the catalog-observed 102.2 kDa expectation.

Which positive control should I start with?

Start with Adipose tissue, the lead HPA protein-expression candidate.

Which negative control is defensible?

Use Spleen as an orthogonal HPA Not detected candidate.

Which gel should I use?

Use 8-10% consistently across the quick facts, protocol table, and poster.

What transfer method to use for CIP2A Western blot?

Use the transfer method in the recommended protocol and verify transfer before blocking.

How should PB9714 be started?

Start at the linked datasheet condition and run a three-point primary-antibody dilution test.

Which publication-derived protocols can I compare?

Compare only the supplied target-verified records: PMC6856592, PMC3105001, PMC5601698.

Boster reagents

CIP2A Western Blot Reagents

Human/Mouse-reactive CIP2A Western blot reagents with authoritative product imagery.

Real WB data Western blot validation image for CIP2A using PB9714; observed band 102.2 kDa

Anti-p90 Autoantigen/KIAA1524 CIP2A Antibody Picoband®

Cat # PB9714

Reactivity: Human, Mouse

View product →

Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.

Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.