Safety Data Sheet (SDS): C-RAF (Phospho-Ser621) Colorimetric Cell-Based ELISA Kit

OSHA/HCS Status

This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).

Hazardous Ingredients

1. -target antibody, -phospho-target antibody, -GAPDH antibody: < 0.1% Sodium azide
2. 10X TBS: 0.2% Kathon CG/ICP
3. 15X Wash buffer: 0.3% Kathon CG/ICP, 1.5% Tween-20
4. HRP-conjugated antibodies: 0.2% Kathon CG/ICP, 0.1% Tween-20
5. Dye 1/2 -conjugated antibodies: 0.2% Kathon CG/ICP, 0.1% Tween-20
6. SDS solution: 1% Sodium Dodecyl Sulfate (SDS)
7. Blocking buffer: 0.5% Triton X-100, 0.1% Tween-20
8. Primary antibody diluent: 0.2% Kathon CG/ICP, 0.5% Triton X-100, 0.1% Tween-20
9. Quenching buffer: 1% Hydrogen peroxide (H2O2)
10. Crystal violet solution: 0.05% crystal violet
11. Ready-to-use substrate: < 0.02% hydrogen peroxide and < 0.1% 3,3',5,5'-Tetramethylbenzidine (TMB)
12. Stop solution: 2N Sulfuric acid (H2SO4)

Classification of the substance or mixture

Sulfuric Acid: Corrosive to Metals (Category 1), H290 Skin corrosion (Category 1A), H314 Serious eye damage (Category 1), H318

Hydrogen Peroxide (quenching buffer): Acute toxicity, Oral (Category 4), H302 Skin irritation (Category 2), H315 Serious eye damage (Category 1), H318 Specific target organ toxicity - single exposure (Category 3), Respiratory system, H335 Short-term (acute) aquatic hazard (Category 2), H401 Long-term (chronic) aquatic hazard (Category 3), H412

Kathon CG/ICP: GHS Classification in accordance with 29 CFR 1910 (OSHA HCS) Skin corrosion (Category 1C), H314 Serious eye damage (Category 1), H318 Skin sensitization (Category 1), H317 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410 For the full text of the H-Statements mentioned in this Section, see Section 16.

Triton X-100: Acute toxicity, Oral (Category 4), H302 Skin irritation (Category 2), H315 Serious eye damage (Category 1), H318 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410

SDS (Sodium dodecyl sulfate): Flammable solids (Category 2), H228 Acute toxicity, Oral (Category 4), H302 Acute toxicity, Inhalation (Category 4), H332 Skin irritation (Category 2), H315 Serious eye damage (Category 1), H318 Specific target organ toxicity - single exposure (Category 3), Respiratory system, H335 Short-term (acute) aquatic hazard (Category 2), H401 Long-term (chronic) aquatic hazard (Category 3), H412

Crystal Violet: Acute toxicity, Oral (Category 4), H302 Serious eye damage (Category 1), H318 Carcinogenicity (Category 2), H351 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410

Sodium azide: Acute toxicity, Oral (Category 2), H300 Acute toxicity, Inhalation (Category 2), H330 Acute toxicity, Dermal (Category 1), H310 Specific target organ toxicity - repeated exposure, Oral (Category 2), Brain, H373 Short-term (acute) aquatic hazard (Category 1), H400 Long-term (chronic) aquatic hazard (Category 1), H410

GHS label elements, including precautionary statements

Hazard pictograms
Signal word/s	Warning      Danger      Danger      Danger

Hazard statements

Sulfuric Acid (Stop Solution): H290 May be corrosive to metals. H314 Causes severe skin burns and eye damage. P264 Wash skin thoroughly after handling.

Hydrogen Peroxide (quenching buffer): H302 Harmful if swallowed. H315 Causes skin irritation. H318 Causes serious eye damage. H335 May cause respiratory irritation. H401 Toxic to aquatic life. H412 Harmful to aquatic life with long lasting effects.

Kathon CG/ICP: H314 Causes severe skin burns and eye damage (H314). H317 May cause an allergic skin reaction. H410 Very toxic to aquatic life with long lasting effects.

Triton X-100: H302 Harmful if swallowed. H315 Causes skin irritation. H318 Causes serious eye damage. H410 Very toxic to aquatic life with long lasting effects.

SDS (Sodium dodecyl sulfate): H228 Flammable solid. H302 + H332 Harmful if swallowed or if inhaled. H315 Causes skin irritation. H318 Causes serious eye damage. H335 May cause respiratory irritation. H401 Toxic to aquatic life. H412 Harmful to aquatic life with long lasting effects.

Crystal Violet: H302 Harmful if swallowed. H318 Causes serious eye damage. H351 Suspected of causing cancer. H410 Very toxic to aquatic life with long lasting effects.

Sodium azide: H300 + H310 + H330 Fatal if swallowed, in contact with skin or if inhaled. H373 May cause damage to organs (Brain) through prolonged or repeated exposure if swallowed. H410 Very toxic to aquatic life with long lasting effects.

Response

IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth. EYE CONTACT: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. SKIN CONTACT: Take off immediately all contaminated clothing. Rinse skin with water/shower. INHALATION: Move to an outside area and breath fresh air. Clear the nose by blowing.

Storage Not applicable.

Disposal Not applicable.

Hazards not otherwise classified

None known.