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Product Info Summary
| SKU: | BA1135 |
|---|---|
| Size: | 0.5 ml |
| Reactive Species: | Rabbit |
| Host: | Goat |
| Application: | Flow Cytometry, IF |
Product info
| SKU | BA1135 |
|---|---|
| Applications | IF, FCM |
| Pack size | 0.5ml |
| Host | Goat |
| Ig Type | IgG |
| Immunogen | Rabbit IgG (whole molecular) |
| Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
| Concentration | 1 mg/ml |
| Specificity | This Fluoro550 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with human/bovine IgG. |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Purity | >99% |
| Contents | 1 mg/ml of Fluoro550 conjugated specific antibody; 0.01M PBS, 5 mg/ml BSA, 50% glycerol. |
| Fluorophore | Amax=562nm; Emax=576nm |
| Form | Concentrated, Liquid |
| Conjugation | Fluoro550 |
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunofluorescence, 2-10 μg/mL
Flow Cytometry, 2-10 μg/mL
Validation Images & Assay Conditions
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IF analysis of PKC gamma using anti-PKC gamma antibody (A01890).
PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Fluoro550 conjugated goat anti-rabbit IgG (BA1035) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For BA1135
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